Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

21771600 .. 21778502

Gene Structure 6903 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1139700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGCTCTGGTGAATGTAACTTCAATCAAAACTGCATCAAGTGGATTATTGCAAGGTGACAATCCTCTAAACTTCGCCTTCCCACTCTTAATCGTCCAAACCACCTTAATCATCGTCGTCAGCCGCTTCCTCGCCTTCCTTCTCAAACCCCTCCGCCAACCCAAAGTCATCGCCGAGATTGCTGGTGGAATATTGCTTGGGCCGTCAGCCTTCGGAAGAAACAAAGACTACCTACACAAAATATTTCCCTCATGGAGCACTCCAATACTCGAAACCGTTGCAAGCATTGGTCTTCTCTTTTTCTTGTTCCTCGTAGGCATTGAGCTCGATTTAAGCTCAATCCGACGTCGTAGTGGCCGAAGTTCATTCGGCATAGCCCTCGCTGGCATATCGGTGCCCTTCCTATGTGGCATCGGCGTCGCTGTGATCCTCCGGAGAACGATCGACGGGGCTGACAAAGTCGGCTTCAGTCAGTTCGTTGTGTTCATGGGAGTATCGCTTTCGATCACGGCCTTCCCTGTACTCGCCCGCATTTTAGCAGAGCTCAAGCTTTTGACTACTAGGGTTGGCGAAACTGCCATGGCTGCTGCCGCGTTGAATGACGTCGCTGCATGGATTTTGCTCGCTCTTGCGGTGGCATTCGCCGGTGACGGCGTTGGTGGCCACAAGAAAAGTCCTCTGGTATCAGTCTGGGTCCTCTTATCAGGTGTTGCATTTGTTGCCTTCATGATGGTGGTGATCCGACCGGCCATGAACTGGGTGGCCCGCCGGAGCTCCCCAGAACAAGATGCCGTAGACGAAGCCTATATATGCCTAACCCTAGCCGGAGTCATGGTATCCGGATTCATTACTGACCTAATTGGTATCCATTCTATATTTGGAGCCTTTGTCTTTGGTCTAACAATTCCCAAAGAAGGACAGTTTGCGGACAGATTGATAGTGAGGACTGAGGACTTTGTGTCCGGCTTGTTGCTTCCACTTTACTTTGCATCAAGTGGGTTGAAGACCGATGTAGCAAAGATACACGGTGCAGAGGCTTGGGGGCTGTTGGTGCTTGTTATATCGATCGCATGTGCCGGGAAGATTTTGGGGACGTTTGTGGTGGCAATGGCGTTTATGTTTCGGGCTAGAGAATCGTTGACGCTTGGTGTGCTCATGAACACCAAAGGGTTGGTGGAGCTCATTGTTCTCAACATTGGCAAGGAGAAAAAGGTGCTTAATGATGAGACATTTGCTATTCTAGTCCTCATGGCACTCTTCACCACCTTTATCACCAGTCCATTAGTGATGGCCATTTACAAGCCCGCACGTGACATCTCAGTTCGCACTCACCGTAAGCTCTGTGATCTGTCAACCACTGAATCCTTCAAGGACGAGCTTCGAGTTCTGGCGTGTGTTCATGGGCCTGCCAATGTGCCCTCACTCATTAGCCTCATTGAGTCCGTCAGAAGTGACAAGAAAGCCCAACTCAAACTCTTCCTCATGCACCTTGTGGAGCTCACTGAGCGGTCTTCGTCCATCATCATGGTCCAACGGGCCCGAAAGAATGGCTTTCCCTTCTTTAATCGATTGGGCGGCGGCCCATGGAGCCACGGCGCTGTTTCCACCTTCAAGGCCTATAGCCAATTGGGCCGAGTCTCGGTCCGACCCACAACCGCAATTTCAGCCCTGTCCACAATGTACGAGGATATTTGTCATGTGGCGGACGACCAGAGGGTGACGATGATCATCTTGCCATTCCACAAACAATGGATGCGTGAGGGCGATAATCATGAAGCGACGGAGGTTGTTGGCCACGGCTGGCGAGGGGTCAACCAGAGGGTGCTTCAAACTGCGCCGTGCTCCGTTGCAGTGCTTGTTGATCGGGGGTTTGGTAACACCGGAGCCCAAACTCCAAGGCCTAGCACCACTGTGACCCGACGAGTTTGCATTGTGTTTTTAGGTGGGCCTGATGATCGCGAGGCCTTGGAGTTTGGCGGTAGGATGGCCGAACACCCGACAGTTAAAGTGACGGTTGTGAAATTTGTTGAGAAAGAGGGCATGGAGAGTAATGGGATCATGTTAAAGCCTTCGCCCTCCAAGTCTACCGAGAATAGCTATTGTTTCTCCACAGCTAAAATGGACCGAAAGAAAGAAAAGGAGCTGGATGAGGAAGCAGTGGCTGAGTTCAGAAGCAAGTGGCACGGGAAAGCAGAGTACATAGAGAAGGTGGCAGCCAATAACATTGTGGAGGGGGTTTTGGAAATAGGAAGAAGTCGAGATCATGACCTGTTAATTGTAGGCAAGGGAAGATTCCCGTCACCCATGGTGGCAGAATTGGCCGACCGCCATGCAGAGCATGCAGAGTTAGGCCCCATTGGAGACATATTGGCTTCATCGAGCCAGGGTGTGGTCTCATCAGTGCTTGTGATTCAACAATATGACGTAGCTCATGCTGTGGAGGCTCCGGTGTCGAAGGTACTGCACGGTGATTACGAAAGGTTCGTCGCGGATGAGTCCTCCAGTTCTGTGCTTGAGATCTCTAAGGAATCTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

846

Amino Acids

91.72

Weight (kDa)

8.66

Isoelectric Point (pI)

38.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	42 - 429	6.9e-60	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	489 - 627	1.5e-34	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	642 - 808	1.6e-16	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	349, 606
AccB1I	GGYRCC	1 cut(s)	394
AccB7I	CCANNNNNTGG	2 cut(s)	756, 2389
AccBSI	CCGCTC	1 cut(s)	1507
AccI	GTMKAC	2 cut(s)	795, 2084
AccII	CGCG	3 cut(s)	593, 1959, 2489
AccIII	TCCGGA	2 cut(s)	432, 839
AclWI	GGATC	3 cut(s)	421, 733, 2063
AcoI	YGGCCR	7 cut(s)	355, 661, 747, 1290, 1792, 1986, 2319
AcsI	RAATTY	1 cut(s)	2021
AcuI	CTGAAG	1 cut(s)	451
AcvI	CACGTG	1 cut(s)	1310
AcyI	GRCGYC	3 cut(s)	346, 417, 603
AdeI	CACNNNGTG	1 cut(s)	1492
AfaI	GTAC	4 cut(s)	522, 1682, 2198, 2460
AfiI	CCNNNNNNNGG	4 cut(s)	756, 914, 1639, 2389
AgsI	TTSAA	7 cut(s)	24, 598, 1003, 1369, 1612, 1829, 2414
AhdI	GACNNNNNGTC	1 cut(s)	958
AjnI	CCWGG	1 cut(s)	2382
AleI	CACNNNNGTG	1 cut(s)	1910
Alw21I	GWGCWC	8 cut(s)	260, 327, 546, 776, 1155, 1182, 1500, 1844
Alw26I	GTCTC	4 cut(s)	1220, 1642, 2355, 2398
AlwI	GGATC	3 cut(s)	421, 733, 2063
AlwNI	CAGNNNCTG	1 cut(s)	2162
Aor13HI	TCCGGA	2 cut(s)	432, 839
ApaI	GGGCCC	1 cut(s)	1540
ApeKI	GCWGC	4 cut(s)	584, 587, 608, 2213
ApoI	RAATTY	1 cut(s)	2021
ArsI	GACNNNNNNTTYG	2 cut(s)	2469, 2501
AspLEI	GCGC	2 cut(s)	1598, 1837
AsuC2I	CCSGG	1 cut(s)	1078
AvaII	GGWCC	4 cut(s)	694, 1528, 1642, 2122
BaeGI	GKGCMC	3 cut(s)	399, 1419, 1540
BalI	TGGCCA	3 cut(s)	663, 1292, 1794
BanI	GGYRCC	1 cut(s)	394
BanII	GRGCYC	7 cut(s)	327, 546, 776, 1182, 1500, 1540, 1888
BbrPI	CACGTG	1 cut(s)	1310
BbsI	GAAGAC	3 cut(s)	284, 1010, 1503
Bbv12I	GWGCWC	8 cut(s)	260, 327, 546, 776, 1155, 1182, 1500, 1844
BbvI	GCAGC	4 cut(s)	571, 574, 595, 2225
BccI	CCATC	4 cut(s)	724, 1282, 1526, 1978
BceAI	ACGGC	7 cut(s)	187, 525, 667, 776, 1609, 1813, 1822
BciT130I	CCWGG	1 cut(s)	2384
BciVI	GTATCC	2 cut(s)	847, 875
BclI	TGATCA	1 cut(s)	1725
BcnI	CCSGG	1 cut(s)	1078
BcoDI	GTCTC	4 cut(s)	1220, 1642, 2355, 2398
BfaI	CTAG	5 cut(s)	561, 821, 1128, 1241, 1902
BfmI	CTRYAG	1 cut(s)	1618
BfoI	RGCGCY	1 cut(s)	1599
BfuI	GTATCC	2 cut(s)	847, 875
BglI	GCCNNNNNGGC	1 cut(s)	1629
BglII	AGATCT	1 cut(s)	2517
BisI	GCNGC	8 cut(s)	124, 585, 588, 591, 609, 1576, 1579, 2214
BlsI	GCNGC	8 cut(s)	125, 586, 589, 592, 610, 1577, 1580, 2215
Bme1390I	CCNGG	2 cut(s)	1078, 2384
Bme18I	GGWCC	4 cut(s)	694, 1528, 1642, 2122
BmeRI	GACNNNNNGTC	1 cut(s)	958
BmiI	GGNNCC	8 cut(s)	396, 695, 883, 1538, 1589, 1885, 2353, 2445
BmrFI	CCNGG	2 cut(s)	1078, 2384
BmrI	ACTGGG	1 cut(s)	766
BmsI	GCATC	5 cut(s)	44, 420, 778, 998, 1743
BmuI	ACTGGG	1 cut(s)	766
BpiI	GAAGAC	3 cut(s)	284, 1010, 1503
BpmI	CTGGAG	1 cut(s)	2485
BpuEI	CTTGAG	2 cut(s)	530, 2534
BpuMI	CCSGG	1 cut(s)	1078
Bsa29I	ATCGAT	2 cut(s)	1064, 1567
BsaAI	YACGTR	1 cut(s)	1310
BsaBI	GATNNNNATC	1 cut(s)	1727
BsaHI	GRCGYC	3 cut(s)	346, 417, 603
BsaI	GGTCTC	1 cut(s)	2398
BsaJI	CCNNGG	8 cut(s)	579, 1583, 1591, 1795, 1895, 1965, 2304, 2383
BsaWI	WCCGGW	4 cut(s)	432, 839, 1880, 2446
BsaXI	ACNNNNNCTCC	4 cut(s)	819, 849, 873, 903
Bsc4I	CCNNNNNNNGG	4 cut(s)	756, 914, 1639, 2389
Bse118I	RCCGGY	2 cut(s)	644, 745
Bse1I	ACTGG	3 cut(s)	761, 1275, 2502
Bse3DI	GCAATG	1 cut(s)	1113
Bse8I	GATNNNNATC	1 cut(s)	1727
BseAI	TCCGGA	2 cut(s)	432, 839
BseBI	CCWGG	1 cut(s)	2384
BseCI	ATCGAT	2 cut(s)	1064, 1567
BseDI	CCNNGG	8 cut(s)	579, 1583, 1591, 1795, 1895, 1965, 2304, 2383
BseGI	GGATG	4 cut(s)	1758, 1989, 2152, 2497
BseJI	GATNNNNATC	1 cut(s)	1727
BseLI	CCNNNNNNNGG	4 cut(s)	756, 914, 1639, 2389
BseMI	GCAATG	1 cut(s)	1113
BseMII	CTCAG	4 cut(s)	939, 1332, 1494, 2154
BseNI	ACTGG	3 cut(s)	761, 1275, 2502
BseSI	GKGCMC	3 cut(s)	399, 1419, 1540
BseXI	GCAGC	4 cut(s)	571, 574, 595, 2225
BsgI	GTGCAG	2 cut(s)	1050, 2447
Bsh1236I	CGCG	3 cut(s)	593, 1959, 2489
Bsh1285I	CGRYCG	4 cut(s)	444, 746, 1068, 2326
BshNI	GGYRCC	1 cut(s)	394
BshVI	ATCGAT	2 cut(s)	1064, 1567
BsiEI	CGRYCG	4 cut(s)	444, 746, 1068, 2326
BsiHKAI	GWGCWC	8 cut(s)	260, 327, 546, 776, 1155, 1182, 1500, 1844
BslFI	GGGAC	1 cut(s)	1105
BslI	CCNNNNNNNGG	4 cut(s)	756, 914, 1639, 2389
BsmAI	GTCTC	4 cut(s)	1220, 1642, 2355, 2398
BsmFI	GGGAC	1 cut(s)	1105
BsmI	GAATGC	1 cut(s)	638
Bso31I	GGTCTC	1 cut(s)	2398
Bsp120I	GGGCCC	1 cut(s)	1536
Bsp13I	TCCGGA	2 cut(s)	432, 839
Bsp19I	CCATGG	3 cut(s)	579, 1583, 2304
Bsp68I	TCGCGA	1 cut(s)	1959
BspCNI	CTCAG	4 cut(s)	940, 1331, 1495, 2155
BspDI	ATCGAT	2 cut(s)	1064, 1567
BspEI	TCCGGA	2 cut(s)	432, 839
BspFNI	CGCG	3 cut(s)	593, 1959, 2489
BspHI	TCATGA	4 cut(s)	726, 1155, 1771, 2263
BspLI	GGNNCC	8 cut(s)	396, 695, 883, 1538, 1589, 1885, 2353, 2445
BspPI	GGATC	3 cut(s)	421, 733, 2063
BspT107I	GGYRCC	1 cut(s)	394
BspTNI	GGTCTC	1 cut(s)	2398
BsrBI	CCGCTC	1 cut(s)	1507
BsrDI	GCAATG	1 cut(s)	1113
BsrFI	RCCGGY	2 cut(s)	644, 745
BsrI	ACTGG	3 cut(s)	761, 1275, 2502
BssAI	RCCGGY	2 cut(s)	644, 745
BssECI	CCNNGG	8 cut(s)	579, 1583, 1591, 1795, 1895, 1965, 2304, 2383
BssNI	GRCGYC	3 cut(s)	346, 417, 603
BssT1I	CCWWGG	5 cut(s)	579, 1583, 1895, 1965, 2304
Bst2UI	CCWGG	1 cut(s)	2384
Bst4CI	ACNGT	8 cut(s)	277, 921, 1028, 1334, 1912, 2002, 2014, 2468
Bst6I	CTCTTC	2 cut(s)	1262, 1481
BstACI	GRCGYC	3 cut(s)	346, 417, 603
BstAPI	GCANNNNNTGC	2 cut(s)	719, 2339
BstBAI	YACGTR	1 cut(s)	1310
BstC8I	GCNNGC	9 cut(s)	283, 385, 529, 624, 766, 1305, 1407, 1802, 2340
BstDEI	CTNAG	5 cut(s)	948, 1318, 1503, 2163, 2523
BstDSI	CCRYGG	5 cut(s)	579, 1583, 1591, 1795, 2304
BstF5I	GGATG	4 cut(s)	1758, 1989, 2152, 2497
BstFNI	CGCG	3 cut(s)	593, 1959, 2489
BstH2I	RGCGCY	1 cut(s)	1599
BstHHI	GCGC	2 cut(s)	1598, 1837
BstMAI	GTCTC	4 cut(s)	1220, 1642, 2355, 2398
BstMCI	CGRYCG	4 cut(s)	444, 746, 1068, 2326
BstNI	CCWGG	1 cut(s)	2384
BstNSI	RCATGY	2 cut(s)	1074, 2342
BstSCI	CCNGG	2 cut(s)	1076, 2382
BstSFI	CTRYAG	1 cut(s)	1618
BstSLI	GKGCMC	3 cut(s)	399, 1419, 1540
BstUI	CGCG	3 cut(s)	593, 1959, 2489
BstV1I	GCAGC	4 cut(s)	571, 574, 595, 2225
BstV2I	GAAGAC	3 cut(s)	284, 1010, 1503
BstX2I	RGATCY	1 cut(s)	2517
BstXI	CCANNNNNNTGG	2 cut(s)	1171, 1525
BstYI	RGATCY	1 cut(s)	2517
Bsu15I	ATCGAT	2 cut(s)	1064, 1567
BsuI	GTATCC	2 cut(s)	847, 875
BsuTUI	ATCGAT	2 cut(s)	1064, 1567
BtgI	CCRYGG	5 cut(s)	579, 1583, 1591, 1795, 2304
BtgZI	GCGATG	1 cut(s)	154
BtsCI	GGATG	4 cut(s)	1758, 1989, 2152, 2497
BtsI	GCAGTG	2 cut(s)	1857, 2163
BtsIMutI	CAGTG	6 cut(s)	1356, 1500, 1857, 1908, 2163, 2406
BtuMI	TCGCGA	1 cut(s)	1959
Cac8I	GCNNGC	9 cut(s)	283, 385, 529, 624, 766, 1305, 1407, 1802, 2340
CaiI	CAGNNNCTG	1 cut(s)	2162
CciI	TCATGA	4 cut(s)	726, 1155, 1771, 2263
CfoI	GCGC	2 cut(s)	1598, 1837
Cfr10I	RCCGGY	2 cut(s)	644, 745
ClaI	ATCGAT	2 cut(s)	1064, 1567
CpoI	CGGWCCG	1 cut(s)	1642
CseI	GACGC	2 cut(s)	406, 1150
Csp6I	GTAC	4 cut(s)	521, 1681, 2197, 2459
CspI	CGGWCCG	1 cut(s)	1642
CviQI	GTAC	4 cut(s)	521, 1681, 2197, 2459
DdeI	CTNAG	5 cut(s)	948, 1318, 1503, 2163, 2523
DraIII	CACNNNGTG	1 cut(s)	1492
DriI	GACNNNNNGTC	1 cut(s)	958
EaeI	YGGCCR	7 cut(s)	355, 661, 747, 1290, 1792, 1986, 2319
Eam1104I	CTCTTC	2 cut(s)	1262, 1481
Eam1105I	GACNNNNNGTC	1 cut(s)	958
EarI	CTCTTC	2 cut(s)	1262, 1481
EciI	GGCGGA	2 cut(s)	143, 1718
Ecl136II	GAGCTC	5 cut(s)	325, 544, 774, 1180, 1498
Eco130I	CCWWGG	5 cut(s)	579, 1583, 1895, 1965, 2304
Eco147I	AGGCCT	3 cut(s)	1616, 1900, 1964
Eco24I	GRGCYC	7 cut(s)	327, 546, 776, 1182, 1500, 1540, 1888
Eco31I	GGTCTC	1 cut(s)	2398
Eco47I	GGWCC	4 cut(s)	694, 1528, 1642, 2122
Eco53kI	GAGCTC	5 cut(s)	325, 544, 774, 1180, 1498
Eco57I	CTGAAG	1 cut(s)	451
Eco72I	CACGTG	1 cut(s)	1310
EcoICRI	GAGCTC	5 cut(s)	325, 544, 774, 1180, 1498
EcoO109I	RGGNCCY	2 cut(s)	694, 2351
EcoRII	CCWGG	1 cut(s)	2382
EcoT14I	CCWWGG	5 cut(s)	579, 1583, 1895, 1965, 2304
EcoT38I	GRGCYC	7 cut(s)	327, 546, 776, 1182, 1500, 1540, 1888
ErhI	CCWWGG	5 cut(s)	579, 1583, 1895, 1965, 2304
FalI	AAGNNNNNCTT	2 cut(s)	1535, 1567
FaqI	GGGAC	1 cut(s)	1105
FauI	CCCGC	3 cut(s)	536, 773, 1312
FbaI	TGATCA	1 cut(s)	1725
FblI	GTMKAC	2 cut(s)	795, 2084
Fnu4HI	GCNGC	8 cut(s)	124, 585, 588, 591, 609, 1576, 1579, 2214
FokI	GGATG	4 cut(s)	1765, 1996, 2159, 2504
FriOI	GRGCYC	7 cut(s)	327, 546, 776, 1182, 1500, 1540, 1888
Fsp4HI	GCNGC	8 cut(s)	124, 585, 588, 591, 609, 1576, 1579, 2214
FspBI	CTAG	5 cut(s)	561, 821, 1128, 1241, 1902
GlaI	GCGC	2 cut(s)	1597, 1836
GluI	GCNGC	8 cut(s)	124, 585, 588, 591, 609, 1576, 1579, 2214
GsuI	CTGGAG	1 cut(s)	2485
HaeII	RGCGCY	1 cut(s)	1599
HgaI	GACGC	2 cut(s)	406, 1150
HhaI	GCGC	2 cut(s)	1598, 1837
Hin1I	GRCGYC	3 cut(s)	346, 417, 603
Hin6I	GCGC	2 cut(s)	1596, 1835
HinP1I	GCGC	2 cut(s)	1596, 1835
HincII	GTYRAC	3 cut(s)	1140, 1353, 1813
HindII	GTYRAC	3 cut(s)	1140, 1353, 1813
HindIII	AAGCTT	1 cut(s)	548
Hpy166II	GTNNAC	6 cut(s)	796, 1140, 1353, 1674, 1813, 2085
Hpy188I	TCNGA	6 cut(s)	215, 344, 743, 1448, 1646, 2171
Hpy188III	TCNNGA	8 cut(s)	433, 727, 840, 1156, 1772, 1958, 2258, 2264
Hpy8I	GTNNAC	6 cut(s)	796, 1140, 1353, 1674, 1813, 2085
Hpy99I	CGWCG	9 cut(s)	119, 348, 351, 422, 449, 608, 1783, 1923, 2489
HpyCH4III	ACNGT	8 cut(s)	277, 921, 1028, 1334, 1912, 2002, 2014, 2468
HpyCH4IV	ACGT	5 cut(s)	346, 603, 1094, 1309, 2424
HpyF3I	CTNAG	5 cut(s)	948, 1318, 1503, 2163, 2523
HpySE526I	ACGT	5 cut(s)	346, 603, 1094, 1309, 2424
Hsp92I	GRCGYC	3 cut(s)	346, 417, 603
HspAI	GCGC	2 cut(s)	1596, 1835
Kpn2I	TCCGGA	2 cut(s)	432, 839
Ksp22I	TGATCA	1 cut(s)	1725
Lsp1109I	GCAGC	4 cut(s)	571, 574, 595, 2225
LweI	GCATC	5 cut(s)	44, 420, 778, 998, 1743
MaeI	CTAG	5 cut(s)	561, 821, 1128, 1241, 1902
MaeII	ACGT	5 cut(s)	346, 603, 1094, 1309, 2424
MbiI	CCGCTC	1 cut(s)	1507
MboII	GAAGA	9 cut(s)	228, 284, 1015, 1093, 1249, 1468, 1503, 2262, 2301
MfeI	CAATTG	1 cut(s)	1625
MflI	RGATCY	1 cut(s)	2517
MlsI	TGGCCA	3 cut(s)	663, 1292, 1794
MluCI	AATT	7 cut(s)	858, 903, 1625, 1659, 2021, 2274, 2315
MluNI	TGGCCA	3 cut(s)	663, 1292, 1794
MlyI	GAGTC	4 cut(s)	837, 1448, 1644, 2504
MmeI	TCCRAC	4 cut(s)	367, 766, 1555, 1669
Mox20I	TGGCCA	3 cut(s)	663, 1292, 1794
MroI	TCCGGA	2 cut(s)	432, 839
MscI	TGGCCA	3 cut(s)	663, 1292, 1794
MseI	TTAA	8 cut(s)	89, 107, 332, 1218, 1563, 2004, 2063, 2273
MslI	CAYNNNNRTG	4 cut(s)	392, 731, 1523, 1910
Msp20I	TGGCCA	3 cut(s)	663, 1292, 1794
MspR9I	CCNGG	2 cut(s)	1078, 2384
MunI	CAATTG	1 cut(s)	1625
Mva1269I	GAATGC	1 cut(s)	638
MvaI	CCWGG	1 cut(s)	2384
MvnI	CGCG	3 cut(s)	593, 1959, 2489
NciI	CCSGG	1 cut(s)	1078
NcoI	CCATGG	3 cut(s)	579, 1583, 2304
NlaIV	GGNNCC	8 cut(s)	396, 695, 883, 1538, 1589, 1885, 2353, 2445
NmeAIII	GCCGAG	2 cut(s)	199, 1658
NmuCI	GTSAC	8 cut(s)	56, 647, 1310, 1451, 1717, 1912, 2008, 2298
NruI	TCGCGA	1 cut(s)	1959
NspI	RCATGY	2 cut(s)	1074, 2342
OliI	CACNNNNGTG	1 cut(s)	1910
PaeI	GCATGC	1 cut(s)	2342
PagI	TCATGA	4 cut(s)	726, 1155, 1771, 2263
PceI	AGGCCT	3 cut(s)	1616, 1900, 1964
PcsI	WCGNNNNNNNCGW	4 cut(s)	355, 1330, 2472, 2481
PctI	GAATGC	1 cut(s)	638
PfeI	GAWTC	6 cut(s)	843, 1133, 1361, 2292, 2410, 2528
PflFI	GACNNNGTC	1 cut(s)	458
PflMI	CCANNNNNTGG	2 cut(s)	756, 2389
PkrI	GCNGC	8 cut(s)	125, 586, 589, 592, 610, 1577, 1580, 2215
Ple19I	CGATCG	2 cut(s)	444, 1068
PleI	GAGTC	4 cut(s)	836, 1447, 1643, 2503
PmaCI	CACGTG	1 cut(s)	1310
PmlI	CACGTG	1 cut(s)	1310
PpsI	GAGTC	4 cut(s)	836, 1447, 1643, 2503
Ppu21I	YACGTR	1 cut(s)	1310
PpuMI	RGGWCCY	1 cut(s)	694
Psp124BI	GAGCTC	5 cut(s)	327, 546, 776, 1182, 1500
Psp5II	RGGWCCY	1 cut(s)	694
Psp6I	CCWGG	1 cut(s)	2382
PspCI	CACGTG	1 cut(s)	1310
PspGI	CCWGG	1 cut(s)	2382
PspN4I	GGNNCC	8 cut(s)	396, 695, 883, 1538, 1589, 1885, 2353, 2445
PspOMI	GGGCCC	1 cut(s)	1536
PspPPI	RGGWCCY	1 cut(s)	694
PstNI	CAGNNNCTG	1 cut(s)	2162
PsuI	RGATCY	1 cut(s)	2517
PsyI	GACNNNGTC	1 cut(s)	458
PvuI	CGATCG	2 cut(s)	444, 1068
RruI	TCGCGA	1 cut(s)	1959
RsaI	GTAC	4 cut(s)	522, 1682, 2198, 2460
RsaNI	GTAC	4 cut(s)	521, 1681, 2197, 2459
RseI	CAYNNNNRTG	4 cut(s)	392, 731, 1523, 1910
Rsr2I	CGGWCCG	1 cut(s)	1642
RsrII	CGGWCCG	1 cut(s)	1642
SacI	GAGCTC	5 cut(s)	327, 546, 776, 1182, 1500
SaqAI	TTAA	8 cut(s)	89, 107, 332, 1218, 1563, 2004, 2063, 2273
SatI	GCNGC	8 cut(s)	124, 585, 588, 591, 609, 1576, 1579, 2214
SchI	GAGTC	4 cut(s)	837, 1448, 1644, 2504
ScrFI	CCNGG	2 cut(s)	1078, 2384
SfaNI	GCATC	5 cut(s)	44, 420, 778, 998, 1743
SfcI	CTRYAG	1 cut(s)	1618
SgrAI	CRCCGGYG	1 cut(s)	644
SinI	GGWCC	4 cut(s)	694, 1528, 1642, 2122
SmiMI	CAYNNNNRTG	4 cut(s)	392, 731, 1523, 1910
SmlI	CTYRAG	2 cut(s)	545, 2513
SmoI	CTYRAG	2 cut(s)	545, 2513
SphI	GCATGC	1 cut(s)	2342
Sse9I	AATT	7 cut(s)	858, 903, 1625, 1659, 2021, 2274, 2315
SseBI	AGGCCT	3 cut(s)	1616, 1900, 1964
SspI	AATATT	2 cut(s)	192, 243
SspMI	CTAG	5 cut(s)	561, 821, 1128, 1241, 1902
SstI	GAGCTC	5 cut(s)	327, 546, 776, 1182, 1500
StuI	AGGCCT	3 cut(s)	1616, 1900, 1964
StyD4I	CCNGG	2 cut(s)	1076, 2382
StyI	CCWWGG	5 cut(s)	579, 1583, 1895, 1965, 2304
TaaI	ACNGT	8 cut(s)	277, 921, 1028, 1334, 1912, 2002, 2014, 2468
TaiI	ACGT	5 cut(s)	349, 606, 1097, 1312, 2427
TaqII	GACCGA	3 cut(s)	1022, 1630, 2139
TasI	AATT	7 cut(s)	858, 903, 1625, 1659, 2021, 2274, 2315
TatI	WGTACW	2 cut(s)	520, 2196
TauI	GCSGC	4 cut(s)	126, 593, 1578, 1581
TfiI	GAWTC	6 cut(s)	843, 1133, 1361, 2292, 2410, 2528
Tru1I	TTAA	8 cut(s)	89, 107, 332, 1218, 1563, 2004, 2063, 2273
Tru9I	TTAA	8 cut(s)	89, 107, 332, 1218, 1563, 2004, 2063, 2273
TscAI	CASTG	6 cut(s)	1363, 1507, 1857, 1915, 2163, 2406
TseFI	GTSAC	8 cut(s)	56, 647, 1310, 1451, 1717, 1912, 2008, 2298
TseI	GCWGC	4 cut(s)	584, 587, 608, 2213
Tsp45I	GTSAC	8 cut(s)	56, 647, 1310, 1451, 1717, 1912, 2008, 2298
TspDTI	ATGAA	9 cut(s)	354, 475, 715, 767, 835, 1172, 1388, 1788, 2364
TspGWI	ACGGA	3 cut(s)	1432, 1796, 1834
TspRI	CASTG	6 cut(s)	1363, 1507, 1857, 1915, 2163, 2406
Tth111I	GACNNNGTC	1 cut(s)	458
Van91I	CCANNNNNTGG	2 cut(s)	756, 2389
VpaK11BI	GGWCC	4 cut(s)	694, 1528, 1642, 2122
XapI	RAATTY	1 cut(s)	2021
XceI	RCATGY	2 cut(s)	1074, 2342
XcmI	CCANNNNNNNNNTGG	5 cut(s)	757, 875, 1171, 2116, 2224
XmiI	GTMKAC	2 cut(s)	795, 2084
XspI	CTAG	5 cut(s)	561, 821, 1128, 1241, 1902
ZraI	GACGTC	2 cut(s)	347, 604

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.