Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr5

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

36705128 .. 36709444

Gene Structure 4317 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000024527

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2352 bp

ATGGAACCTATGGTTCTGAACCTAACCAGCATGAGAACTTCTTCAGATGGAGCTTGGCAGGGTGATAATCCTCTGAACCATGCCTTCCCATTGTTGATAGTTCAAACCACTCTGGTCCTCTTCATCAGTCGCTTCTTCGCCTTCATTCTCAAACCACTCCGCCAACCTAAGGTCATTGCCGAGATCTTAGGTGGGATTTTGCTAGGACCTTCTGCACTTGGCCGGATCAAAGATTTCTCCCACATCATATTCCCTTCATGGAGCACTCCCATACTCGAATCAGTAGCAAGCATCGGCCTCCTCTTCTACTTATTCCTCGTAGGCCTCGAGCTCGACTTGCGCTCCATTCGCCGCAGTGGCAAAAGCGCCATCACCATAGCACTCGCAGGAATTTCCCTCCCTTTCCTCATCGCAGTAGGAGTCACATTTCTCATGCGCAAGGCCATCAACGGTGAGAACAAAGTAGGGTACCCTCAGTTCCTCCTGTTCATAGGGGTAGCCCTCTCCATCACCGCCTTCCCTGTCCTCGCACGCATCTTAGCAGAGCTCAAACTGCTCACCACACTCATAGGTGAGACGGCAATGGCTGCAGCAGCCTTCAACGACGTGGCTGCATGGATTCTGCTGGCCTTGGCCGTGGCCTTAGCCGGTGGGAACCATAAAGGCCCTCTAGTCTCCATTTGGGTTCTCATTTCCGGCGTGGCTTTTCTTGCTTTTATGTTCATTCTGGTCCGTCCTTTGATGATTTGGGTTGCTAACCGTTGCTCATCTCAAATTGAGAACGAAGCTTATATATGCCTCACCCTAGCCGGGGTCATGTTGTCCGGGTTCATGACTGACCTAATCGGTGTCCATGCCATCTTTGGGGCATTTATTTTTGGATTAACCATTCCGAAAGGGGGTGAATTCGCAGCCAAACTGACTAAGAGGATCGAGGATTTTGTGTCTGGTTTGCTTCTCCCTCTCTACTTTGCCTCCAGTGGTTTGAAGACTGACGTTACTAAAATACATGGGATCAAGGCATGGGGTCTTTTGGCTTTGGTTATATCAGTTTCTTGCACTGGAAAAATTTTGGGAACGTTTTTGGTTGCAATGATGTGTATGATACCAGTGAGGGAGTCATTAGCTCTAGGCGTACTGATGAATACTAAAGGATTGGTGGAGCTTATTGTTCTGAATATTGGCAAGGAGAAAAAGATTCTAAACGACGAGATGTTTGCTATTTTTGTGCTGATGGCACTTTTCACCACCTTCATGACGACACCAATAGTGATGGCCATTTACAAACCCTATGTCGTTGCTTCCCAATCTGGGAGTAGTTTAGAGAGACAGAATTTTCCTAGCAAGAACTCGAAGAAAAAGAAGGAACTTCGAATCCTTGCTTGTATTCATGGACCGGGAAGTGTACCCTCCCTTATCAACCTCATTGAGTCAACCGGAACCTCCTCCGAGAATTCTCTTCTCAAAGTTTACGTGATGCATCTTGTTGAACTTACTGATCGTTCATCCTCCATTATGATGGTACACAAAGTTCGGAAAAACGGCTTGCCCTTCATCAACCGCTTTTGTCTAGGGGTGGCACGCCATATCGCCTCAGCATTTGAAGCTTATAGTCAAGTTGGTCAAGTCACCATCCGTCACACAACAGCCATCTCGGCTTTGTCTACGATGCACGAGGATATTCTCCATGTTGCGGAAGCTAAGAGGGTGTCCATCATTATCTTGCCTTTCAACAAACAGAGGAATAAACAGGGTGAAGAGACGATGGATAATTTAGAGCATCGCTGGAAAGCAGTCAATGAGAGAGTTCTAACATGTGCTAATTGTTCGGTAGCATTGTTTGTGGATCGTGGATTCAGTGTTGGAGCTGAGAAGGGTGCAATGACCTTAAGCGCTACCAATCTGAAGAGGGTGTGCGTTCTGTTCTTGGGTGGACCTGATGACCGCAGGGCACTGGAGTATGTCGGGGGAATTGCAAATCAAAACGTTGTAATTACTGTTGTTAGGTGTTTGAAGCAGAGCACTACTCCTCTAAGCCCCATCACCAACCCCGACAGGGAAATTGATGAGACTGCAGTAGCAGAGTTTCTTAGGCAGAATGGGTCGGTGGAATTCATAGAGAAAGTGGTGGTCGGCAACATTATGGACACGGTGGCTGGCATAGTTCAGAGCAGGGAATACAACCTGGTGATCGTGGGGAAGCAACACCACCCTTCAACAATTGTTGCAGAATTGTCTCGCAATCAGCTGGAGCATACCGAACTGGGGCCAATTGGAGACGGATTAGCTTCATCCTCGCACGTGATTTCGCGCTCCGTTCTTGTGATACAAACACCACAGATTAGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

784

Amino Acids

85.18

Weight (kDa)

9.27

Isoelectric Point (pI)

37.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	41 - 424	3.1e-61	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	488 - 624	3.2e-32	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	638 - 777	1.7e-08	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	437
Acc65I	GGTACC	1 cut(s)	468
AccB1I	GGYRCC	1 cut(s)	468
AccI	GTMKAC	1 cut(s)	1664
AccII	CGCG	1 cut(s)	2311
AciI	CCGC	6 cut(s)	160, 352, 513, 1561, 1694, 1945
AclI	AACGTT	2 cut(s)	1079, 1986
AclWI	GGATC	4 cut(s)	233, 938, 1022, 1854
AcoI	YGGCCR	3 cut(s)	220, 633, 1275
AcsI	RAATTY	6 cut(s)	390, 905, 1068, 1333, 1453, 2111
AcuI	CTGAAG	2 cut(s)	27, 1925
AcvI	CACGTG	1 cut(s)	2302
AfaI	GTAC	4 cut(s)	470, 1137, 1407, 1524
AfeI	AGCGCT	1 cut(s)	1894
AfiI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1311, 1693, 2056, 2265
AflII	CTTAAG	1 cut(s)	1888
AflIII	ACRYGT	1 cut(s)	1814
AgsI	TTSAA	8 cut(s)	104, 601, 988, 1490, 1604, 1732, 2014, 2217
AjiI	CACGTC	1 cut(s)	607
AjnI	CCWGG	1 cut(s)	2184
AloI	GAACNNNNNNTCC	2 cut(s)	1359, 1391
Alw21I	GWGCWC	4 cut(s)	266, 333, 549, 2024
Alw26I	GTCTC	7 cut(s)	569, 679, 1321, 1754, 2063, 2241, 2271
AlwI	GGATC	4 cut(s)	233, 938, 1022, 1854
Ama87I	CYCGRG	1 cut(s)	326
Aor51HI	AGCGCT	1 cut(s)	1894
ApeKI	GCWGC	5 cut(s)	587, 590, 593, 611, 911
ApoI	RAATTY	6 cut(s)	390, 905, 1068, 1333, 1453, 2111
ArsI	GACNNNNNNTTYG	4 cut(s)	1199, 1231, 1278, 1310
Asp700I	GAANNNNTTC	1 cut(s)	40
Asp718I	GGTACC	1 cut(s)	468
AspLEI	GCGC	5 cut(s)	342, 368, 438, 1895, 2313
AspS9I	GGNCC	7 cut(s)	115, 206, 665, 730, 1394, 1934, 2267
AsuC2I	CCSGG	3 cut(s)	811, 826, 1398
AsuII	TTCGAA	1 cut(s)	1372
AvaI	CYCGRG	1 cut(s)	326
AvaII	GGWCC	5 cut(s)	115, 206, 730, 1394, 1934
AxyI	CCTNAGG	1 cut(s)	168
BaeGI	GKGCMC	1 cut(s)	1954
BalI	TGGCCA	1 cut(s)	1277
BanI	GGYRCC	1 cut(s)	468
BanII	GRGCYC	2 cut(s)	333, 549
BauI	CACGAG	1 cut(s)	1673
BbrPI	CACGTG	1 cut(s)	2302
BbsI	GAAGAC	1 cut(s)	995
Bbv12I	GWGCWC	4 cut(s)	266, 333, 549, 2024
BbvCI	CCTCAGC	1 cut(s)	1594
BbvI	GCAGC	5 cut(s)	574, 598, 602, 605, 923
BceAI	ACGGC	3 cut(s)	594, 620, 1558
BcgI	CGANNNNNNTGC	4 cut(s)	593, 627, 1199, 1233
BciT130I	CCWGG	1 cut(s)	2186
BcnI	CCSGG	3 cut(s)	811, 826, 1398
BcoDI	GTCTC	7 cut(s)	569, 679, 1321, 1754, 2063, 2241, 2271
BfaI	CTAG	6 cut(s)	203, 671, 806, 1130, 1341, 1571
BfmI	CTRYAG	2 cut(s)	588, 2073
BfoI	RGCGCY	2 cut(s)	369, 1896
BfrI	CTTAAG	1 cut(s)	1888
BglI	GCCNNNNNGGC	2 cut(s)	357, 1655
BglII	AGATCT	1 cut(s)	183
BisI	GCNGC	6 cut(s)	352, 588, 591, 594, 612, 912
BlsI	GCNGC	6 cut(s)	353, 589, 592, 595, 613, 913
Bme1390I	CCNGG	4 cut(s)	811, 826, 1398, 2186
Bme18I	GGWCC	5 cut(s)	115, 206, 730, 1394, 1934
BmeT110I	CYCGRG	1 cut(s)	326
BmgBI	CACGTC	1 cut(s)	607
BmgT120I	GGNCC	7 cut(s)	115, 206, 665, 730, 1394, 1934, 2267
BmiI	GGNNCC	5 cut(s)	6, 470, 656, 1441, 2268
BmrFI	CCNGG	4 cut(s)	811, 826, 1398, 2186
BmrI	ACTGGG	1 cut(s)	2273
BmsI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
BmuI	ACTGGG	1 cut(s)	2273
BpiI	GAAGAC	1 cut(s)	995
BplI	GAGNNNNNCTC	2 cut(s)	2011, 2043
BpmI	CTGGAG	3 cut(s)	961, 1976, 2270
Bpu10I	CCTNAGC	2 cut(s)	643, 1594
Bpu14I	TTCGAA	1 cut(s)	1372
BpuMI	CCSGG	3 cut(s)	811, 826, 1398
BsaAI	YACGTR	2 cut(s)	1474, 2302
BsaJI	CCNNGG	3 cut(s)	630, 636, 810
BsaWI	WCCGGW	1 cut(s)	1436
BsaXI	ACNNNNNCTCC	4 cut(s)	326, 356, 959, 989
Bsc4I	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1311, 1693, 2056, 2265
Bse118I	RCCGGY	1 cut(s)	647
Bse1I	ACTGG	5 cut(s)	978, 1066, 1109, 1959, 2268
Bse21I	CCTNAGG	1 cut(s)	168
Bse3DI	GCAATG	4 cut(s)	174, 588, 1098, 1887
BseBI	CCWGG	1 cut(s)	2186
BseDI	CCNNGG	3 cut(s)	630, 636, 810
BseGI	GGATG	3 cut(s)	1505, 1632, 2291
BseLI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1311, 1693, 2056, 2265
BseMI	GCAATG	4 cut(s)	174, 588, 1098, 1887
BseMII	CTCAG	3 cut(s)	488, 1608, 1860
BseNI	ACTGG	5 cut(s)	978, 1066, 1109, 1959, 2268
BseRI	GAGGAG	3 cut(s)	290, 1435, 2019
BseSI	GKGCMC	1 cut(s)	1954
BseXI	GCAGC	5 cut(s)	574, 598, 602, 605, 923
BsgI	GTGCAG	1 cut(s)	198
Bsh1236I	CGCG	1 cut(s)	2311
BshNI	GGYRCC	1 cut(s)	468
BsiHKAI	GWGCWC	4 cut(s)	266, 333, 549, 2024
BsiHKCI	CYCGRG	1 cut(s)	326
BsiSI	CCGG	7 cut(s)	223, 648, 696, 810, 825, 1397, 1437
BslI	CCNNNNNNNGG	9 cut(s)	169, 810, 811, 864, 899, 1311, 1693, 2056, 2265
BsmAI	GTCTC	7 cut(s)	569, 679, 1321, 1754, 2063, 2241, 2271
BsmBI	CGTCTC	3 cut(s)	569, 1754, 2271
BsoBI	CYCGRG	1 cut(s)	326
Bsp119I	TTCGAA	1 cut(s)	1372
Bsp1286I	GDGCHC	5 cut(s)	266, 333, 549, 1954, 2024
Bsp143I	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
BspACI	CCGC	6 cut(s)	160, 352, 513, 1561, 1694, 1945
BspCNI	CTCAG	3 cut(s)	487, 1607, 1861
BspFNI	CGCG	1 cut(s)	2311
BspHI	TCATGA	2 cut(s)	831, 1254
BspLI	GGNNCC	5 cut(s)	6, 470, 656, 1441, 2268
BspMAI	CTGCAG	2 cut(s)	592, 2077
BspPI	GGATC	4 cut(s)	233, 938, 1022, 1854
BspT104I	TTCGAA	1 cut(s)	1372
BspT107I	GGYRCC	1 cut(s)	468
BspTI	CTTAAG	1 cut(s)	1888
BsrDI	GCAATG	4 cut(s)	174, 588, 1098, 1887
BsrFI	RCCGGY	1 cut(s)	647
BsrI	ACTGG	5 cut(s)	978, 1066, 1109, 1959, 2268
BssAI	RCCGGY	1 cut(s)	647
BssECI	CCNNGG	3 cut(s)	630, 636, 810
BssMI	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
BssSI	CACGAG	1 cut(s)	1673
BssT1I	CCWWGG	1 cut(s)	630
Bst2BI	CACGAG	1 cut(s)	1673
Bst2UI	CCWGG	1 cut(s)	2186
Bst4CI	ACNGT	4 cut(s)	452, 761, 1999, 2152
Bst6I	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1901
BstAFI	CTTAAG	1 cut(s)	1888
BstAPI	GCANNNNNTGC	1 cut(s)	587
BstBAI	YACGTR	2 cut(s)	1474, 2302
BstBI	TTCGAA	1 cut(s)	1372
BstC8I	GCNNGC	6 cut(s)	289, 532, 627, 1547, 1582, 2158
BstDSI	CCRYGG	1 cut(s)	636
BstF5I	GGATG	3 cut(s)	1505, 1632, 2291
BstFNI	CGCG	1 cut(s)	2311
BstH2I	RGCGCY	2 cut(s)	369, 1896
BstHHI	GCGC	5 cut(s)	342, 368, 438, 1895, 2313
BstKTI	GATC	7 cut(s)	186, 228, 933, 1017, 1501, 1849, 2193
BstMAI	GTCTC	7 cut(s)	569, 679, 1321, 1754, 2063, 2241, 2271
BstMBI	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
BstMWI	GCNNNNNNNGC	9 cut(s)	337, 348, 357, 553, 587, 593, 710, 1604, 1655
BstNI	CCWGG	1 cut(s)	2186
BstNSI	RCATGY	1 cut(s)	1818
BstSCI	CCNGG	4 cut(s)	809, 824, 1396, 2184
BstSFI	CTRYAG	2 cut(s)	588, 2073
BstSLI	GKGCMC	1 cut(s)	1954
BstUI	CGCG	1 cut(s)	2311
BstV1I	GCAGC	5 cut(s)	574, 598, 602, 605, 923
BstV2I	GAAGAC	1 cut(s)	995
BstX2I	RGATCY	1 cut(s)	183
BstXI	CCANNNNNNTGG	1 cut(s)	1519
BstYI	RGATCY	1 cut(s)	183
Bsu36I	CCTNAGG	1 cut(s)	168
BtgI	CCRYGG	1 cut(s)	636
BtgZI	GCGATG	2 cut(s)	394, 1766
BtrI	CACGTC	1 cut(s)	607
BtsCI	GGATG	3 cut(s)	1505, 1632, 2291
BtsI	GCAGTG	1 cut(s)	361
BtsIMutI	CAGTG	6 cut(s)	361, 985, 1059, 1116, 1864, 1952
Cac8I	GCNNGC	6 cut(s)	289, 532, 627, 1547, 1582, 2158
CciI	TCATGA	2 cut(s)	831, 1254
CfoI	GCGC	5 cut(s)	342, 368, 438, 1895, 2313
Cfr10I	RCCGGY	1 cut(s)	647
Cfr13I	GGNCC	7 cut(s)	115, 206, 665, 730, 1394, 1934, 2267
CsiI	ACCWGGT	1 cut(s)	2184
Csp6I	GTAC	4 cut(s)	469, 1136, 1406, 1523
CviQI	GTAC	4 cut(s)	469, 1136, 1406, 1523
DpnI	GATC	7 cut(s)	185, 227, 932, 1016, 1500, 1848, 2192
DpnII	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
EaeI	YGGCCR	3 cut(s)	220, 633, 1275
Eam1104I	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1901
EarI	CTCTTC	5 cut(s)	125, 308, 1464, 1752, 1901
EciI	GGCGGA	1 cut(s)	149
Ecl136II	GAGCTC	2 cut(s)	331, 547
Eco130I	CCWWGG	1 cut(s)	630
Eco147I	AGGCCT	1 cut(s)	324
Eco24I	GRGCYC	2 cut(s)	333, 549
Eco47I	GGWCC	5 cut(s)	115, 206, 730, 1394, 1934
Eco47III	AGCGCT	1 cut(s)	1894
Eco53kI	GAGCTC	2 cut(s)	331, 547
Eco57I	CTGAAG	2 cut(s)	27, 1925
Eco72I	CACGTG	1 cut(s)	2302
Eco81I	CCTNAGG	1 cut(s)	168
Eco88I	CYCGRG	1 cut(s)	326
EcoICRI	GAGCTC	2 cut(s)	331, 547
EcoO109I	RGGNCCY	2 cut(s)	206, 665
EcoRI	GAATTC	3 cut(s)	905, 1453, 2111
EcoRII	CCWGG	1 cut(s)	2184
EcoT14I	CCWWGG	1 cut(s)	630
EcoT22I	ATGCAT	1 cut(s)	1482
EcoT38I	GRGCYC	2 cut(s)	333, 549
ErhI	CCWWGG	1 cut(s)	630
Esp3I	CGTCTC	3 cut(s)	569, 1754, 2271
FblI	GTMKAC	1 cut(s)	1664
Fnu4HI	GCNGC	6 cut(s)	352, 588, 591, 594, 612, 912
FokI	GGATG	3 cut(s)	1492, 1619, 2278
FriOI	GRGCYC	2 cut(s)	333, 549
Fsp4HI	GCNGC	6 cut(s)	352, 588, 591, 594, 612, 912
FspBI	CTAG	6 cut(s)	203, 671, 806, 1130, 1341, 1571
FspI	TGCGCA	1 cut(s)	437
GlaI	GCGC	5 cut(s)	341, 367, 437, 1894, 2312
GluI	GCNGC	6 cut(s)	352, 588, 591, 594, 612, 912
GsuI	CTGGAG	3 cut(s)	961, 1976, 2270
HaeII	RGCGCY	2 cut(s)	369, 1896
HapII	CCGG	7 cut(s)	223, 648, 696, 810, 825, 1397, 1437
HhaI	GCGC	5 cut(s)	342, 368, 438, 1895, 2313
Hin6I	GCGC	5 cut(s)	340, 366, 436, 1893, 2311
HinP1I	GCGC	5 cut(s)	340, 366, 436, 1893, 2311
HincII	GTYRAC	1 cut(s)	1434
HindII	GTYRAC	1 cut(s)	1434
HindIII	AAGCTT	2 cut(s)	786, 1605
HinfI	GANTC	8 cut(s)	278, 420, 619, 1118, 1198, 1374, 1430, 1854
HpaII	CCGG	7 cut(s)	223, 648, 696, 810, 825, 1397, 1437
Hpy166II	GTNNAC	6 cut(s)	1406, 1434, 1471, 1525, 1665, 1934
Hpy188I	TCNGA	9 cut(s)	18, 46, 75, 894, 1176, 1450, 1536, 1905, 2169
Hpy188III	TCNNGA	2 cut(s)	832, 1255
Hpy8I	GTNNAC	6 cut(s)	1406, 1434, 1471, 1525, 1665, 1934
Hpy99I	CGWCG	2 cut(s)	608, 1211
HpyCH4III	ACNGT	4 cut(s)	452, 761, 1999, 2152
HpyCH4IV	ACGT	6 cut(s)	606, 996, 1079, 1473, 1986, 2301
HpyF10VI	GCNNNNNNNGC	9 cut(s)	337, 348, 357, 553, 587, 593, 710, 1604, 1655
HpySE526I	ACGT	6 cut(s)	606, 996, 1079, 1473, 1986, 2301
HspAI	GCGC	5 cut(s)	340, 366, 436, 1893, 2311
KpnI	GGTACC	1 cut(s)	472
Kzo9I	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
LmnI	GCTCC	7 cut(s)	50, 261, 347, 1162, 1865, 2251, 2318
Lsp1109I	GCAGC	5 cut(s)	574, 598, 602, 605, 923
LweI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
MabI	ACCWGGT	1 cut(s)	2184
MaeI	CTAG	6 cut(s)	203, 671, 806, 1130, 1341, 1571
MaeII	ACGT	6 cut(s)	606, 996, 1079, 1473, 1986, 2301
MaeIII	GTNAC	4 cut(s)	421, 997, 1627, 1637
MalI	GATC	7 cut(s)	185, 227, 932, 1016, 1500, 1848, 2192
MboI	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
MboII	GAAGA	9 cut(s)	33, 112, 127, 295, 1000, 1366, 1451, 1769, 1918
MfeI	CAATTG	2 cut(s)	2220, 2271
MflI	RGATCY	1 cut(s)	183
MhlI	GDGCHC	5 cut(s)	266, 333, 549, 1954, 2024
MlsI	TGGCCA	1 cut(s)	1277
MluNI	TGGCCA	1 cut(s)	1277
MlyI	GAGTC	3 cut(s)	429, 1127, 1439
MmeI	TCCRAC	1 cut(s)	1843
Mox20I	TGGCCA	1 cut(s)	1277
Mph1103I	ATGCAT	1 cut(s)	1482
MroXI	GAANNNNTTC	1 cut(s)	40
MscI	TGGCCA	1 cut(s)	1277
MseI	TTAA	2 cut(s)	884, 1889
MslI	CAYNNNNRTG	2 cut(s)	1253, 1517
Msp20I	TGGCCA	1 cut(s)	1277
MspA1I	CMGCKG	1 cut(s)	2248
MspCI	CTTAAG	1 cut(s)	1888
MspI	CCGG	7 cut(s)	223, 648, 696, 810, 825, 1397, 1437
MspR9I	CCNGG	4 cut(s)	811, 826, 1398, 2186
MunI	CAATTG	2 cut(s)	2220, 2271
MvaI	CCWGG	1 cut(s)	2186
MvnI	CGCG	1 cut(s)	2311
MwoI	GCNNNNNNNGC	9 cut(s)	337, 348, 357, 553, 587, 593, 710, 1604, 1655
NciI	CCSGG	3 cut(s)	811, 826, 1398
NdeII	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
NlaIV	GGNNCC	5 cut(s)	6, 470, 656, 1441, 2268
NmeAIII	GCCGAG	2 cut(s)	205, 1634
NmuCI	GTSAC	3 cut(s)	421, 1627, 1637
NsbI	TGCGCA	1 cut(s)	437
NsiI	ATGCAT	1 cut(s)	1482
NspI	RCATGY	1 cut(s)	1818
NspV	TTCGAA	1 cut(s)	1372
PaeR7I	CTCGAG	1 cut(s)	326
PagI	TCATGA	2 cut(s)	831, 1254
PceI	AGGCCT	1 cut(s)	324
PciI	ACATGT	1 cut(s)	1814
PcsI	WCGNNNNNNNCGW	1 cut(s)	324
PdmI	GAANNNNTTC	1 cut(s)	40
PfeI	GAWTC	5 cut(s)	278, 619, 1198, 1374, 1854
PkrI	GCNGC	6 cut(s)	353, 589, 592, 595, 613, 913
PleI	GAGTC	3 cut(s)	428, 1126, 1438
PmaCI	CACGTG	1 cut(s)	2302
PmlI	CACGTG	1 cut(s)	2302
PpsI	GAGTC	3 cut(s)	428, 1126, 1438
Ppu21I	YACGTR	2 cut(s)	1474, 2302
PpuMI	RGGWCCY	1 cut(s)	206
PscI	ACATGT	1 cut(s)	1814
Psp124BI	GAGCTC	2 cut(s)	333, 549
Psp1406I	AACGTT	2 cut(s)	1079, 1986
Psp5II	RGGWCCY	1 cut(s)	206
Psp6I	CCWGG	1 cut(s)	2184
PspCI	CACGTG	1 cut(s)	2302
PspGI	CCWGG	1 cut(s)	2184
PspN4I	GGNNCC	5 cut(s)	6, 470, 656, 1441, 2268
PspPI	GGNCC	7 cut(s)	115, 206, 665, 730, 1394, 1934, 2267
PspPPI	RGGWCCY	1 cut(s)	206
PspXI	VCTCGAGB	1 cut(s)	326
PsrI	GAACNNNNNNTAC	4 cut(s)	461, 493, 1515, 1547
PstI	CTGCAG	2 cut(s)	592, 2077
PsuI	RGATCY	1 cut(s)	183
PvuII	CAGCTG	1 cut(s)	2248
RsaI	GTAC	4 cut(s)	470, 1137, 1407, 1524
RsaNI	GTAC	4 cut(s)	469, 1136, 1406, 1523
RseI	CAYNNNNRTG	2 cut(s)	1253, 1517
SacI	GAGCTC	2 cut(s)	333, 549
SaqAI	TTAA	2 cut(s)	884, 1889
SatI	GCNGC	6 cut(s)	352, 588, 591, 594, 612, 912
Sau3AI	GATC	7 cut(s)	183, 225, 930, 1014, 1498, 1846, 2190
Sau96I	GGNCC	7 cut(s)	115, 206, 665, 730, 1394, 1934, 2267
SchI	GAGTC	3 cut(s)	429, 1127, 1439
ScrFI	CCNGG	4 cut(s)	811, 826, 1398, 2186
SduI	GDGCHC	5 cut(s)	266, 333, 549, 1954, 2024
SexAI	ACCWGGT	1 cut(s)	2184
SfaNI	GCATC	6 cut(s)	300, 543, 1467, 1489, 1659, 1789
SfcI	CTRYAG	2 cut(s)	588, 2073
Sfr274I	CTCGAG	1 cut(s)	326
SfuI	TTCGAA	1 cut(s)	1372
SinI	GGWCC	5 cut(s)	115, 206, 730, 1394, 1934
SlaI	CTCGAG	1 cut(s)	326
SmiMI	CAYNNNNRTG	2 cut(s)	1253, 1517
SmlI	CTYRAG	2 cut(s)	326, 1888
SmoI	CTYRAG	2 cut(s)	326, 1888
SseBI	AGGCCT	1 cut(s)	324
SsiI	CCGC	6 cut(s)	160, 352, 513, 1561, 1694, 1945
SspI	AATATT	1 cut(s)	1180
SspMI	CTAG	6 cut(s)	203, 671, 806, 1130, 1341, 1571
SstI	GAGCTC	2 cut(s)	333, 549
StuI	AGGCCT	1 cut(s)	324
StyD4I	CCNGG	4 cut(s)	809, 824, 1396, 2184
StyI	CCWWGG	1 cut(s)	630
TaaI	ACNGT	4 cut(s)	452, 761, 1999, 2152
TaiI	ACGT	6 cut(s)	609, 999, 1082, 1476, 1989, 2304
TaqI	TCGA	6 cut(s)	276, 327, 333, 933, 1352, 1372
TauI	GCSGC	1 cut(s)	354
TfiI	GAWTC	5 cut(s)	278, 619, 1198, 1374, 1854
Tru1I	TTAA	2 cut(s)	884, 1889
Tru9I	TTAA	2 cut(s)	884, 1889
TscAI	CASTG	6 cut(s)	361, 985, 1066, 1116, 1864, 1959
TseFI	GTSAC	3 cut(s)	421, 1627, 1637
TseI	GCWGC	5 cut(s)	587, 590, 593, 611, 911
Tsp45I	GTSAC	3 cut(s)	421, 1627, 1637
TspGWI	ACGGA	4 cut(s)	722, 1625, 2295, 2305
TspRI	CASTG	6 cut(s)	361, 985, 1066, 1116, 1864, 1959
Vha464I	CTTAAG	1 cut(s)	1888
VpaK11BI	GGWCC	5 cut(s)	115, 206, 730, 1394, 1934
XapI	RAATTY	6 cut(s)	390, 905, 1068, 1333, 1453, 2111
XceI	RCATGY	1 cut(s)	1818
XcmI	CCANNNNNNNNNTGG	1 cut(s)	860
XhoI	CTCGAG	1 cut(s)	326
XmiI	GTMKAC	1 cut(s)	1664
XmnI	GAANNNNTTC	1 cut(s)	40
XspI	CTAG	6 cut(s)	203, 671, 806, 1130, 1341, 1571
Zsp2I	ATGCAT	1 cut(s)	1482

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.