Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Reverse (-)

14084148 .. 14087258

Gene Structure 3111 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g11220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2316 bp

ATGGTACTCAATTTTACCAACTTGAGAACTTCATCTGATGGAGCTTGGCAAGGTGATAATCCTCTTCACCATGCCTTCCCATTATTAATAGTTCAAACCCTTCTCATCCTCTTCGTTAGCCGCTTCCTCGCCTTCCTTCTCAAGCCACTCCGCCAACCTAAAGTCATTGCTGAGATATTAGGTGGGATTTTGTTGGGACCATCCGCACTTGGCAGGAATAAAGATTTCTTGGAGATCTTATTCCCTTCATGGAGCACTCCCATACTAGAATCCGTTGCAAGCATTGGCCTTCTCTTTTACCTATTTCTCGTCGGCCTCGAGCTTGATCTGATCTCCATTCGCCGCAGTGGCAGAAGCTCCATCAGCATAGCACTCGCAGGCATTTCCCTCCCCTTCTTCTTCGGAGCTGGTCTCGCATTCTTTACCCGAAAAGTCATCCACGGAGAGAACAAGGTCGGTTACCCTCAGCTTCTCGTGTTCACAGGGGTAGCCCTCTCCATCACCGCTTTCCCTGTCCTCGCTCGCATCTTAGCGGAGCTCAAACTTCTCACAACTCGTGTCGGTGAAACTGCGATGGCAGCAGCAGCCTTCAACGATATAGCTGCATGGATCCTGCTGGCATTGGCGGTGGCCTTAGCCGGCGGGAGCCACAAAAGCCCCTTAGTGTCCATTTGGGTTCTCATTTCCGGCGTTGGTTTTGTTGTTTTTCAGCTAACTTTGGTCCGACCTTTCATGAACTGGGTTGCCAAACGGTGCTCATCCGATCATGACCTAGTGGATGAGGCATACATCTGCTTAACCCTAGCCGGGGTACTGTTATCCGGGTTTATGACAGACATGATCGGCATCCATGCCATATTCGGCGCTTTTGTTTTCGGGTTAACGATTCCAAAGGGGGGAGAGTTTGCAGCCAGACTGACCAAAAGGATAGAGGATTTTGTGTCTGGCTTGCTTCTTCCTCTATATTTTGCTTCAAGTGGTTTAAAAACTGATGTTGCTAAAATTCAAGGGCTCAGGGCATGGGGGATGGTGGCTTTGGTCATATCTGTGTCCTGCACTGGCAAGATTTTGGGTACTTTTCTGGTGGCCATGTTATTTATGATACCAGTAAGAGAGTCATTAGCACTGGGAGTCCTCATGAATACTAAAGGCTTGGTGGAGCTCATTGTTCTCAACATTGGGAAGGAAAAAAAGATTCTAAACGATGAGATGTTTGCTATTTTGACCCTGATGGCAGTTTTTACCACCTTCATGACAACTCCAGCAGTGATGGCTATTTACAAACCCTCATCGGATGTCATTGCTTCTCAAACTCCCCGTCTCTTACAGGGACAACATTCTCCGACCAAACCCGCCCACAGACGAAAGGACCTTCGAATCCTTGCTTGCGTTCATGGACCTGGAAGTGTACCTTCCCTCATCAACCTCATCGAGTCAGTCGCACCTGCCTCACAACACTCATTGCCGCTCAAAGTTTACGTGATGCATCTTGTTGAGCTCACTGACCGTTCATCATCCATTATGATGGTACATCGAGCTCGGAAAAATGGTTTTCCCTTCATCAACCGCTTATGTCGAGGGGTCTCACAGGACCAAATCCCTGCGGCGTTTGAGGCCTATGGTCAAGTCGGTCAAGTCACCATCCGCCACACGACGGCCATCTCCGCGTTGTCCACAATGCAAGAGGATATTCTCCATGTTGCAGAGGCGAAGACGGTTTCCCTCGTCATCTTGCCTTTTAACAAACAATGGAGAAGAGAGGAAGAGAAGGAAATGGAGGATCTGGGGGATGACTGGAAAAGGGTCAATCAGAGGGTCTTAAGCACTGCAAATTGCTCAGTGGCATTGTTCGTGGATCGTGGATTTGGCAGCAGACGTGAGAGGGGTGCCGCCACTCCCAAGAGGGTGTGCATTTTGTTCTTGGGTGGACCTGACGACCGCGAGGCTCTGGAATTTGGTGGAAGATTGGCACATTCAAGCATTATGTTAACAGTTGTAAAATGCATTAGGGACAGCTTGGAGCAAAATCATGAGAAGGAACTAGATGAGGCAGCAGTTGCAGAGTTGAGGAGGTGTTATGAATCAGTGGAATACATAGAGAAGGTGGTGACAGGCGGCATCGCGGGGACAGCGGTGAGTATAGGGCTGAGTGGGGAATACGACCTGGTTATCGTGGGGAAGGAGCAGCAGAATTCTGAATTGGCTGGCCATCAGCTGGAGCATGCTGAACTGGGACAAATTGGAGACACATTAGCATCATCACCTGGGATTTCGTCCTCTGTTCTTGTGATACAAACACCAAGAACCCGCACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

772

Amino Acids

83.95

Weight (kDa)

8.71

Isoelectric Point (pI)

39.19

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1453
Acc36I	ACCTGC	1 cut(s)	1453
AccB1I	GGYRCC	1 cut(s)	1886
AccB7I	CCANNNNNTGG	1 cut(s)	2215
AccBSI	CCGCTC	1 cut(s)	1468
AccII	CGCG	3 cut(s)	1667, 1941, 2123
AclWI	GGATC	4 cut(s)	604, 617, 1788, 1863
AcoI	YGGCCR	3 cut(s)	1086, 1656, 2206
AcsI	RAATTY	3 cut(s)	1002, 1952, 2191
AfaI	GTAC	5 cut(s)	6, 813, 1075, 1410, 1530
AfiI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
AflII	CTTAAG	1 cut(s)	1819
AgsI	TTSAA	5 cut(s)	95, 592, 975, 1007, 1977
AjiI	CACGTC	1 cut(s)	1877
AjnI	CCWGG	3 cut(s)	1399, 2163, 2263
AjuI	GAANNNNNNNTTGG	2 cut(s)	2183, 2215
Alw21I	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
Alw26I	GTCTC	4 cut(s)	416, 1325, 1588, 2238
AlwI	GGATC	4 cut(s)	604, 617, 1788, 1863
Ama87I	CYCGRG	1 cut(s)	317
AoxI	GGCC	7 cut(s)	286, 313, 630, 1086, 1614, 1656, 2206
ApeKI	GCWGC	8 cut(s)	578, 581, 584, 602, 908, 1869, 2051, 2185
ApoI	RAATTY	3 cut(s)	1002, 1952, 2191
ArsI	GACNNNNNNTTYG	2 cut(s)	1303, 1335
AseI	ATTAAT	1 cut(s)	86
AspLEI	GCGC	1 cut(s)	866
AspS9I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
AsuC2I	CCSGG	2 cut(s)	808, 823
AsuHPI	GGTGA	8 cut(s)	59, 65, 493, 575, 1630, 2119, 2146, 2253
AsuII	TTCGAA	1 cut(s)	1375
AvaI	CYCGRG	1 cut(s)	317
AvaII	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BalI	TGGCCA	2 cut(s)	1088, 2208
BamHI	GGATCC	1 cut(s)	609
BanI	GGYRCC	1 cut(s)	1886
BanII	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
BauI	CACGAG	2 cut(s)	473, 555
BbsI	GAAGAC	1 cut(s)	1718
Bbv12I	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
BbvCI	CCTCAGC	1 cut(s)	465
BbvI	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BceAI	ACGGC	1 cut(s)	1671
BcgI	CGANNNNNNTGC	4 cut(s)	355, 389, 584, 618
BciT130I	CCWGG	3 cut(s)	1401, 2165, 2265
BcnI	CCSGG	2 cut(s)	808, 823
BcoDI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BfaI	CTAG	4 cut(s)	266, 773, 803, 2042
BfoI	RGCGCY	1 cut(s)	867
BfrI	CTTAAG	1 cut(s)	1819
BfuAI	ACCTGC	1 cut(s)	1453
BglI	GCCNNNNNGGC	1 cut(s)	348
BglII	AGATCT	1 cut(s)	234
Bme1390I	CCNGG	5 cut(s)	808, 823, 1401, 2165, 2265
Bme18I	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BmeT110I	CYCGRG	1 cut(s)	317
BmgBI	CACGTC	1 cut(s)	1877
BmgT120I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
BmiI	GGNNCC	4 cut(s)	198, 611, 647, 1888
BmrFI	CCNGG	5 cut(s)	808, 823, 1401, 2165, 2265
BmrI	ACTGGG	3 cut(s)	748, 1136, 2240
BmsI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
BmuI	ACTGGG	3 cut(s)	748, 1136, 2240
BpiI	GAAGAC	1 cut(s)	1718
BplI	GAGNNNNNCTC	2 cut(s)	396, 428
BpmI	CTGGAG	2 cut(s)	1245, 2237
Bpu10I	CCTNAGC	3 cut(s)	465, 634, 1013
Bpu14I	TTCGAA	1 cut(s)	1375
BpuEI	CTTGAG	2 cut(s)	43, 125
BpuMI	CCSGG	2 cut(s)	808, 823
BsaAI	YACGTR	1 cut(s)	1480
BsaBI	GATNNNNATC	1 cut(s)	845
BsaI	GGTCTC	2 cut(s)	416, 1588
BsaJI	CCNNGG	3 cut(s)	439, 807, 2264
Bsc4I	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
Bse118I	RCCGGY	1 cut(s)	638
Bse1I	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
Bse3DI	GCAATG	3 cut(s)	165, 1299, 1460
Bse8I	GATNNNNATC	1 cut(s)	845
BseBI	CCWGG	3 cut(s)	1401, 2165, 2265
BseDI	CCNNGG	3 cut(s)	439, 807, 2264
BseJI	GATNNNNATC	1 cut(s)	845
BseLI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
BseMI	GCAATG	3 cut(s)	165, 1299, 1460
BseMII	CTCAG	5 cut(s)	162, 479, 1027, 1851, 2138
BseNI	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
BseRI	GAGGAG	1 cut(s)	2083
BseXI	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BsgI	GTGCAG	1 cut(s)	1039
Bsh1236I	CGCG	3 cut(s)	1667, 1941, 2123
Bsh1285I	CGRYCG	1 cut(s)	1939
BshFI	GGCC	7 cut(s)	288, 315, 632, 1088, 1616, 1658, 2208
BshNI	GGYRCC	1 cut(s)	1886
BsiEI	CGRYCG	1 cut(s)	1939
BsiHKAI	GWGCWC	6 cut(s)	257, 540, 758, 1164, 1500, 1540
BsiHKCI	CYCGRG	1 cut(s)	317
BsiSI	CCGG	4 cut(s)	639, 687, 807, 822
BslFI	GGGAC	5 cut(s)	210, 1344, 2024, 2140, 2247
BslI	CCNNNNNNNGG	5 cut(s)	807, 808, 896, 1654, 2215
BsmAI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BsmBI	CGTCTC	1 cut(s)	1325
BsmFI	GGGAC	5 cut(s)	210, 1344, 2024, 2140, 2247
BsmI	GAATGC	1 cut(s)	416
BsnI	GGCC	7 cut(s)	288, 315, 632, 1088, 1616, 1658, 2208
Bso31I	GGTCTC	2 cut(s)	416, 1588
BsoBI	CYCGRG	1 cut(s)	317
Bsp119I	TTCGAA	1 cut(s)	1375
Bsp1286I	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
Bsp143I	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
BspANI	GGCC	7 cut(s)	288, 315, 632, 1088, 1616, 1658, 2208
BspCNI	CTCAG	5 cut(s)	163, 478, 1026, 1850, 2139
BspFNI	CGCG	3 cut(s)	1667, 1941, 2123
BspHI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
BspLI	GGNNCC	4 cut(s)	198, 611, 647, 1888
BspMI	ACCTGC	1 cut(s)	1453
BspPI	GGATC	4 cut(s)	604, 617, 1788, 1863
BspT104I	TTCGAA	1 cut(s)	1375
BspT107I	GGYRCC	1 cut(s)	1886
BspTI	CTTAAG	1 cut(s)	1819
BspTNI	GGTCTC	2 cut(s)	416, 1588
BsrBI	CCGCTC	1 cut(s)	1468
BsrDI	GCAATG	3 cut(s)	165, 1299, 1460
BsrFI	RCCGGY	1 cut(s)	638
BsrI	ACTGG	6 cut(s)	743, 1063, 1106, 1131, 1799, 2235
BssAI	RCCGGY	1 cut(s)	638
BssECI	CCNNGG	3 cut(s)	439, 807, 2264
BssMI	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
BssSI	CACGAG	2 cut(s)	473, 555
Bst2BI	CACGAG	2 cut(s)	473, 555
Bst2UI	CCWGG	3 cut(s)	1401, 2165, 2265
Bst4CI	ACNGT	5 cut(s)	753, 816, 1508, 1717, 1993
Bst6I	CTCTTC	4 cut(s)	69, 116, 1750, 1758
BstAFI	CTTAAG	1 cut(s)	1819
BstAPI	GCANNNNNTGC	1 cut(s)	2057
BstBAI	YACGTR	1 cut(s)	1480
BstBI	TTCGAA	1 cut(s)	1375
BstC8I	GCNNGC	9 cut(s)	280, 379, 523, 618, 640, 950, 1387, 2206, 2223
BstDEI	CTNAG	8 cut(s)	171, 465, 529, 634, 661, 1013, 1837, 2147
BstDSI	CCRYGG	1 cut(s)	439
BstEII	GGTNACC	1 cut(s)	458
BstFNI	CGCG	3 cut(s)	1667, 1941, 2123
BstH2I	RGCGCY	1 cut(s)	867
BstHHI	GCGC	1 cut(s)	866
BstKTI	GATC	8 cut(s)	237, 328, 333, 612, 766, 843, 1783, 1858
BstMAI	GTCTC	4 cut(s)	416, 1325, 1588, 2238
BstMBI	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
BstMCI	CGRYCG	1 cut(s)	1939
BstNI	CCWGG	3 cut(s)	1401, 2165, 2265
BstNSI	RCATGY	1 cut(s)	2225
BstPI	GGTNACC	1 cut(s)	458
BstSCI	CCNGG	5 cut(s)	806, 821, 1399, 2163, 2263
BstUI	CGCG	3 cut(s)	1667, 1941, 2123
BstV1I	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
BstV2I	GAAGAC	1 cut(s)	1718
BstX2I	RGATCY	3 cut(s)	234, 609, 1780
BstXI	CCANNNNNNTGG	1 cut(s)	1525
BstYI	RGATCY	3 cut(s)	234, 609, 1780
BsuRI	GGCC	7 cut(s)	288, 315, 632, 1088, 1616, 1658, 2208
BtgI	CCRYGG	1 cut(s)	439
BtgZI	GCGATG	2 cut(s)	587, 2104
BtrI	CACGTC	1 cut(s)	1877
BtsI	GCAGTG	3 cut(s)	352, 1272, 1824
BtsIMutI	CAGTG	8 cut(s)	352, 1056, 1124, 1272, 1500, 1824, 1845, 2091
BveI	ACCTGC	1 cut(s)	1453
Cac8I	GCNNGC	9 cut(s)	280, 379, 523, 618, 640, 950, 1387, 2206, 2223
CciI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
CfoI	GCGC	1 cut(s)	866
Cfr10I	RCCGGY	1 cut(s)	638
Cfr13I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
CsiI	ACCWGGT	1 cut(s)	2163
Csp6I	GTAC	5 cut(s)	5, 812, 1074, 1409, 1529
CviQI	GTAC	5 cut(s)	5, 812, 1074, 1409, 1529
DdeI	CTNAG	8 cut(s)	171, 465, 529, 634, 661, 1013, 1837, 2147
DpnI	GATC	8 cut(s)	236, 327, 332, 611, 765, 842, 1782, 1857
DpnII	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
DraI	TTTAAA	1 cut(s)	984
EaeI	YGGCCR	3 cut(s)	1086, 1656, 2206
Eam1104I	CTCTTC	4 cut(s)	69, 116, 1750, 1758
EarI	CTCTTC	4 cut(s)	69, 116, 1750, 1758
EciI	GGCGGA	2 cut(s)	140, 1634
Ecl136II	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
Eco147I	AGGCCT	1 cut(s)	1616
Eco24I	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
Eco31I	GGTCTC	2 cut(s)	416, 1588
Eco47I	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
Eco53kI	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
Eco88I	CYCGRG	1 cut(s)	317
Eco91I	GGTNACC	1 cut(s)	458
EcoICRI	GAGCTC	4 cut(s)	538, 1162, 1498, 1538
EcoO109I	RGGNCCY	1 cut(s)	1369
EcoO65I	GGTNACC	1 cut(s)	458
EcoRI	GAATTC	1 cut(s)	2191
EcoRII	CCWGG	3 cut(s)	1399, 2163, 2263
EcoT22I	ATGCAT	2 cut(s)	1488, 2006
EcoT38I	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
Esp3I	CGTCTC	1 cut(s)	1325
FalI	AAGNNNNNCTT	2 cut(s)	1396, 1428
FaqI	GGGAC	5 cut(s)	210, 1344, 2024, 2140, 2247
FauI	CCCGC	3 cut(s)	635, 1360, 2116
FriOI	GRGCYC	5 cut(s)	540, 1014, 1164, 1500, 1540
FspBI	CTAG	4 cut(s)	266, 773, 803, 2042
GlaI	GCGC	1 cut(s)	865
GsuI	CTGGAG	2 cut(s)	1245, 2237
HaeII	RGCGCY	1 cut(s)	867
HaeIII	GGCC	7 cut(s)	288, 315, 632, 1088, 1616, 1658, 2208
HapII	CCGG	4 cut(s)	639, 687, 807, 822
HhaI	GCGC	1 cut(s)	866
Hin6I	GCGC	1 cut(s)	864
HinP1I	GCGC	1 cut(s)	864
HincII	GTYRAC	2 cut(s)	882, 1989
HindII	GTYRAC	2 cut(s)	882, 1989
HinfI	GANTC	8 cut(s)	269, 886, 1115, 1131, 1195, 1377, 1433, 2081
HpaI	GTTAAC	2 cut(s)	882, 1989
HpaII	CCGG	4 cut(s)	639, 687, 807, 822
HphI	GGTGA	8 cut(s)	59, 65, 493, 575, 1630, 2119, 2146, 2253
Hpy166II	GTNNAC	7 cut(s)	480, 882, 1409, 1477, 1674, 1928, 1989
Hpy188III	TCNNGA	6 cut(s)	733, 767, 1138, 1252, 1949, 2030
Hpy8I	GTNNAC	7 cut(s)	480, 882, 1409, 1477, 1674, 1928, 1989
Hpy99I	CGWCG	2 cut(s)	314, 1657
HpyCH4III	ACNGT	5 cut(s)	753, 816, 1508, 1717, 1993
HpyCH4IV	ACGT	2 cut(s)	1479, 1876
HpyF3I	CTNAG	8 cut(s)	171, 465, 529, 634, 661, 1013, 1837, 2147
HpySE526I	ACGT	2 cut(s)	1479, 1876
HspAI	GCGC	1 cut(s)	864
KroI	GCCGGC	1 cut(s)	638
KroNI	GCCGGC	1 cut(s)	640
KspAI	GTTAAC	2 cut(s)	882, 1989
Kzo9I	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
Lsp1109I	GCAGC	8 cut(s)	589, 590, 593, 596, 920, 1881, 2063, 2197
LweI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
MabI	ACCWGGT	1 cut(s)	2163
MaeI	CTAG	4 cut(s)	266, 773, 803, 2042
MaeII	ACGT	2 cut(s)	1479, 1876
MaeIII	GTNAC	3 cut(s)	458, 1636, 2107
MalI	GATC	8 cut(s)	236, 327, 332, 611, 765, 842, 1782, 1857
MbiI	CCGCTC	1 cut(s)	1468
MboI	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
MboII	GAAGA	9 cut(s)	56, 103, 388, 391, 947, 1723, 1767, 1775, 1974
MflI	RGATCY	3 cut(s)	234, 609, 1780
MhlI	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
MlsI	TGGCCA	2 cut(s)	1088, 2208
MluCI	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
MluNI	TGGCCA	2 cut(s)	1088, 2208
MlyI	GAGTC	3 cut(s)	1124, 1140, 1442
MmeI	TCCRAC	2 cut(s)	748, 1367
Mox20I	TGGCCA	2 cut(s)	1088, 2208
Mph1103I	ATGCAT	2 cut(s)	1488, 2006
MroNI	GCCGGC	1 cut(s)	638
MscI	TGGCCA	2 cut(s)	1088, 2208
MseI	TTAA	7 cut(s)	86, 797, 881, 983, 1740, 1820, 1988
MslI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
Msp20I	TGGCCA	2 cut(s)	1088, 2208
MspA1I	CMGCKG	2 cut(s)	2132, 2215
MspCI	CTTAAG	1 cut(s)	1819
MspI	CCGG	4 cut(s)	639, 687, 807, 822
MspR9I	CCNGG	5 cut(s)	808, 823, 1401, 2165, 2265
Mva1269I	GAATGC	1 cut(s)	416
MvaI	CCWGG	3 cut(s)	1401, 2165, 2265
MvnI	CGCG	3 cut(s)	1667, 1941, 2123
NaeI	GCCGGC	1 cut(s)	640
NciI	CCSGG	2 cut(s)	808, 823
NdeII	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
NgoMIV	GCCGGC	1 cut(s)	638
NlaIV	GGNNCC	4 cut(s)	198, 611, 647, 1888
NmuCI	GTSAC	2 cut(s)	1636, 2107
NsiI	ATGCAT	2 cut(s)	1488, 2006
NspI	RCATGY	1 cut(s)	2225
NspV	TTCGAA	1 cut(s)	1375
PaeI	GCATGC	1 cut(s)	2225
PaeR7I	CTCGAG	1 cut(s)	317
PagI	TCATGA	5 cut(s)	732, 766, 1137, 1251, 2029
PaqCI	CACCTGC	1 cut(s)	1453
PceI	AGGCCT	1 cut(s)	1616
PcsI	WCGNNNNNNNCGW	1 cut(s)	315
PctI	GAATGC	1 cut(s)	416
PdiI	GCCGGC	1 cut(s)	640
PfeI	GAWTC	5 cut(s)	269, 886, 1195, 1377, 2081
PflMI	CCANNNNNTGG	1 cut(s)	2215
PleI	GAGTC	3 cut(s)	1123, 1139, 1441
PpsI	GAGTC	3 cut(s)	1123, 1139, 1441
Ppu21I	YACGTR	1 cut(s)	1480
PpuMI	RGGWCCY	1 cut(s)	1369
PshBI	ATTAAT	1 cut(s)	86
Psp124BI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
Psp5II	RGGWCCY	1 cut(s)	1369
Psp6I	CCWGG	3 cut(s)	1399, 2163, 2263
PspEI	GGTNACC	1 cut(s)	458
PspGI	CCWGG	3 cut(s)	1399, 2163, 2263
PspN4I	GGNNCC	4 cut(s)	198, 611, 647, 1888
PspPI	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
PspPPI	RGGWCCY	1 cut(s)	1369
PspXI	VCTCGAGB	1 cut(s)	317
PsuI	RGATCY	3 cut(s)	234, 609, 1780
PvuII	CAGCTG	1 cut(s)	2215
RsaI	GTAC	5 cut(s)	6, 813, 1075, 1410, 1530
RsaNI	GTAC	5 cut(s)	5, 812, 1074, 1409, 1529
RseI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
SacI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
SaqAI	TTAA	7 cut(s)	86, 797, 881, 983, 1740, 1820, 1988
Sau3AI	GATC	8 cut(s)	234, 325, 330, 609, 763, 840, 1780, 1855
Sau96I	GGNCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
SchI	GAGTC	3 cut(s)	1124, 1140, 1442
ScrFI	CCNGG	5 cut(s)	808, 823, 1401, 2165, 2265
SduI	GDGCHC	7 cut(s)	257, 540, 758, 1014, 1164, 1500, 1540
SexAI	ACCWGGT	1 cut(s)	2163
SfaNI	GCATC	6 cut(s)	534, 855, 1473, 1495, 2127, 2264
Sfr274I	CTCGAG	1 cut(s)	317
SfuI	TTCGAA	1 cut(s)	1375
SinI	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
SlaI	CTCGAG	1 cut(s)	317
SmiMI	CAYNNNNRTG	3 cut(s)	1046, 1250, 1523
SmlI	CTYRAG	4 cut(s)	22, 140, 317, 1819
SmoI	CTYRAG	4 cut(s)	22, 140, 317, 1819
SphI	GCATGC	1 cut(s)	2225
Sse9I	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
SseBI	AGGCCT	1 cut(s)	1616
SspMI	CTAG	4 cut(s)	266, 773, 803, 2042
SstI	GAGCTC	4 cut(s)	540, 1164, 1500, 1540
StuI	AGGCCT	1 cut(s)	1616
StyD4I	CCNGG	5 cut(s)	806, 821, 1399, 2163, 2263
TaaI	ACNGT	5 cut(s)	753, 816, 1508, 1717, 1993
TaiI	ACGT	2 cut(s)	1482, 1879
TaqI	TCGA	5 cut(s)	318, 1375, 1431, 1534, 1576
TaqII	GACCGA	1 cut(s)	1619
TasI	AATT	7 cut(s)	10, 1002, 1831, 1952, 2191, 2198, 2238
TauI	GCSGC	6 cut(s)	123, 345, 1468, 1607, 1892, 2118
TfiI	GAWTC	5 cut(s)	269, 886, 1195, 1377, 2081
Tru1I	TTAA	7 cut(s)	86, 797, 881, 983, 1740, 1820, 1988
Tru9I	TTAA	7 cut(s)	86, 797, 881, 983, 1740, 1820, 1988
TscAI	CASTG	8 cut(s)	352, 1063, 1131, 1272, 1507, 1831, 1845, 2091
TseFI	GTSAC	2 cut(s)	1636, 2107
TseI	GCWGC	8 cut(s)	578, 581, 584, 602, 908, 1869, 2051, 2185
Tsp45I	GTSAC	2 cut(s)	1636, 2107
TspGWI	ACGGA	2 cut(s)	262, 456
TspRI	CASTG	8 cut(s)	352, 1063, 1131, 1272, 1507, 1831, 1845, 2091
Van91I	CCANNNNNTGG	1 cut(s)	2215
Vha464I	CTTAAG	1 cut(s)	1819
VpaK11BI	GGWCC	6 cut(s)	197, 721, 1369, 1397, 1591, 1928
VspI	ATTAAT	1 cut(s)	86
XapI	RAATTY	3 cut(s)	1002, 1952, 2191
XceI	RCATGY	1 cut(s)	2225
XhoI	CTCGAG	1 cut(s)	317
XspI	CTAG	4 cut(s)	266, 773, 803, 2042
Zsp2I	ATGCAT	2 cut(s)	1488, 2006

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.