Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0002802

Physical Location & Seq

Genomic Coordinates Forward (+)

13321701 .. 13326992

Gene Structure 5292 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ43304

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGGTGAATATAACCACCATCAGAACAGCATCAAATGGCCTGTGGCAAGGCGACAACCCTTTAAACTTCGCCTTCCCACTCCTAATAGTTCAGACCACCTTGATCATCGTCGTCAGCCGCTTCCTTGCTTTCCTACTGAAACCCCTCCACCAACCCAAAGTCATTGCAGAGATTGTCGGTGGAATTCTGCTTGGTCCTTCAGCTTTCGGACGAAACATAGATTACTTGCACAAAATCTTTCCTTCACGGGGCACTCCAATATTAGAATCAGTGGCAAGTATTGGTCTTCTATTTTACTTGTTCCTTGTAGGCTTAGAGCTCGACCTGAGCTCCACTCGCCGAAGTGGCAGAACGGGTTTCAGCATAGCCCTCGCCGGCATAACGCTCCCTTTCCTCTTCGGCATCGGCGTCGCTCTGGTCTTCCGTAAAACAATTGATGGAGCTGACAAAGCAGGGTTCACTCAGTTCCTTGTCTTCATGGGAGTTGCCCTCTCCATCACCGCCTTCCCTGTGCTTGCCCGCATCTTAGCGGAGCTCAAACTGTTAACAACTCAGATTGGGGAGACTGCCATGGCGGCTGCTGCCGTCAACGACGTTGGTGCATGGATCTTGCTGGCCGTGGCCGTGGCCCTGGCAGGTGACGGTGCTGGTGGCCACAACAAGAGCCCTTTGATATGCATTTGGGTCCTCCTATCAGGTGTTGCCTTTGTGGCCTTCATGATGGTCGTGATCCGACCTGCCATGAAATGGGTCGCCAGCCGGTGCACACCGGAGCAAGATGTCTTGGACGAAGCCTATATTTGCTTAACCTTAACTGGAGTAATGGTGGCCGGGTTCATGACTGACATAATTGGAATTCATTCCATATTTGGAGCTTTCGTCTTCGGACTCACAATTCCGAAAGGACAGTTTGCAGATAGATTAATATCGAGGATTGAAGACTTTGTGTCCGGCTTGTTGCTTCCGCTTTACTTTGCATCAAGTGGGTTGAAAACAGATGTGGCTAAAATACGTGGCGGCAGGGCATGGGGGTTGTTGGCTCTGGTTATAACCACGGCTTGCGCTGGGAAGATTCTCGGGACGTTTGTGGTGGCAATGATGTTTATGATTCCGGCCAGAGAGTCATTGACGCTTGGTGTGCTCATGAACACTAAAGGCTTGGTAGAGCTCATTGTTCTAAACATTGGCAAGGAGCAGAAGGTGCTAAATGACGAGTCATTTGCAATTCTAGTCCTCATGGCACTCTCCACCACCTTCATTACATGTCCGGTAGTGATGGCCATTTACAGACCGGCACGTGGAATCTCTATTCGCACTCACCGCAAACTTTGTGATCTCTCCACAGCTGATCAAGCCGCCAAAGACGAGCTCCGAATTCTTGCATGTGTTCATGGTCCAAACAATGCACCCTCGCTCATAAGCTTAATAGATTCAATTCGAAGCACCAAAAAATCCCAACTCAAACTCTTCCTAATGCACCTTGTTGAACTCACCGAGCGCTCTTCTTCAATCGTTATGGTCCAACGGGCTCGAAAAAATGGGTTTCCTTTCTTTAATCGAACGGCTCGCGGTGAGTGGTATGATAGGGTAGCTGGGGCATTCCAAGCCTATAGTCAATTGGGCCGAGTCACGGTCCGGCCCACAACTGCAATTTCGGCTTTGTCCACAATGTATGAGGATATTTGCCATGCGGCGGAAGAGAAGAGGGCGGCGATGATCGTCTTGCCATTCCACAAGCAATGGAGATGTGATGGTGATGATCATGAGAAAAAAGAGATTGATGTGGGCAATGATTGGAGAGGGGTCAACCAAAGAGTGCTCCAAAATGCGCCGTGCTCGGTTGCAGTGCTTGTGGACCGTGGATTTGATAATTTCAAAGCCCAAAGTCTCGAGCCGGATAAGCTTGTGGCCCTAAGGATTTGTATTGTGTTTTTGGGTGGGCCTGATGATCGAGAGGCCTTGGAATTGGGTGGTCGGATGGCTGACCACCCAGCGGTTAAAGTGAAGGTTGTGAGGTTCGTCAAGAAAGAAGGTCTGGAGAGTAGTAATGGGTCTCATGGGCTTATGTTAAAGCCTTCACCCACCAAGTCCACCGAGAACAATTGTAGCTTCTCCACAGCCATAATGGACCGAGAGAATGAAAAGGAACTAGATGAGGCTGCAGTGGCTGAGTTCAGAAGCAAGTTGGTGGAGGATGGGACGGCAGAGTACACTGAGCTAGTGGTGGCTAGGAATATTGTGGAGGGGGTGTTGGCAATTGGACGCAGTGGAGATCAAGATCTAATAATAGTAGGAAAAGGACGGTTCCCATCAAGTTTGGTAGCCGAATTGGCAGATCGACAAGCGGAGCACGCTGAGCTGGGGCCTGTCGGAGACATTTTGGCCTATTCCGGCCACGATGTGGTCTCATCAATCCTTGTGATTCAACAGCACAATGTAGCTCATGCGGTGGAAGCTCCAGTGTCCAAGATGGTGCGTGGTGAGTATGAAAAGTTTGCCCCTGATGAATCCTCCAGTGTACTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006605 GO:0006623 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0006885 GO:0006886 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015672 GO:0015833 GO:0019725 GO:0030003 GO:0030004 GO:0030007 GO:0030104 GO:0033036 GO:0033365 GO:0034613 GO:0042592 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0065007 GO:0065008 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072665 GO:0072666 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

842

Amino Acids

91.28

Weight (kDa)

7.59

Isoelectric Point (pI)

33.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	40 - 421	3.6e-54	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	486 - 623	7.6e-38	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	638 - 811	1.7e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002802)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G53720 AT3G53720
fragaria_vesca	FvH4_6g14141 FvH4_6g14141 FvH4_6g14150
malus_domestica	MD04G1124400.v1.1 MD04G1124500.v1.1 MD04G1124700.v1.1 MD12G1139700.v1.1
prunus_persica	Prupe.6G251600_v2.0.a1 Prupe.6G251700_v2.0.a1 Prupe.6G251700_v2.0.a1
pyrus_communis	pycom04g11220 pycom04g11230 pycom12g13420
rosa_chinensis	RchiOBHm_Chr3g0467061 RchiOBHm_Chr3g0467081 RchiOBHm_Chr3g0467091
rosa_laevigata	RLG00000024527 RLG00000024528 RLG00000024532
rosa_multiflora	Rmu_co8287377.1_g000001 Rmu_co8419643.1_g000001 Rmu_sc0012393.1_g000001 Rmu_ssc0000065.1_g000004
rosa_roxburghii	Rroxscaffold_6G00413900 Rroxscaffold_6G00413920
rosa_rugosa	Rorug03G0085100 Rorug03G0085400
rosa_samantha	Rh3AG133100 Rh3AG133400 Rh3BG154200 Rh3BG154600 Rh3CG154200 Rh3CG154300 Rh3DG155200 Rh3DG155400
rosa_wichuraiana	Rw3G012600 Rw3G012610

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1049
AarI	CACCTGC	1 cut(s)	626
Acc36I	ACCTGC	2 cut(s)	626, 745
AccB7I	CCANNNNNTGG	2 cut(s)	747, 2401
AccII	CGCG	1 cut(s)	1569
AclWI	GGATC	2 cut(s)	614, 724
AcoI	YGGCCR	7 cut(s)	615, 621, 652, 828, 1113, 1278, 2392
AcsI	RAATTY	3 cut(s)	183, 855, 1374
AcuI	CTGAAG	1 cut(s)	183
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	1 cut(s)	408
AdeI	CACNNNGTG	1 cut(s)	2401
AfaI	GTAC	2 cut(s)	2210, 2520
AfeI	AGCGCT	1 cut(s)	1499
AfiI	CCNNNNNNNGG	7 cut(s)	248, 747, 1298, 1630, 1693, 1993, 2401
AflIII	ACRYGT	1 cut(s)	1262
AgsI	TTSAA	7 cut(s)	938, 991, 1434, 1487, 1509, 1876, 2426
AhdI	GACNNNNNGTC	1 cut(s)	946
AjnI	CCWGG	1 cut(s)	630
Alw26I	GTCTC	5 cut(s)	557, 1892, 2058, 2367, 2410
Alw44I	GTGCAC	1 cut(s)	763
AlwI	GGATC	2 cut(s)	614, 724
AlwNI	CAGNNNCTG	1 cut(s)	2168
Ama87I	CYCGRG	2 cut(s)	1076, 1889
Aor51HI	AGCGCT	1 cut(s)	1499
ApaLI	GTGCAC	1 cut(s)	763
ApeKI	GCWGC	3 cut(s)	578, 581, 2159
ApoI	RAATTY	3 cut(s)	183, 855, 1374
ArsI	GACNNNNNNTTYG	4 cut(s)	1202, 1234, 1847, 1879
AseI	ATTAAT	1 cut(s)	923
Asp700I	GAANNNNTTC	1 cut(s)	859
AspLEI	GCGC	3 cut(s)	1064, 1500, 1831
AsuC2I	CCSGG	1 cut(s)	832
AsuHPI	GGTGA	9 cut(s)	16, 490, 650, 1310, 1483, 1583, 1766, 2070, 2492
AsuII	TTCGAA	1 cut(s)	1438
AvaI	CYCGRG	2 cut(s)	1076, 1889
AvaII	GGWCC	7 cut(s)	194, 685, 1394, 1519, 1633, 1855, 2128
AxyI	CCTNAGG	1 cut(s)	1913
BaeGI	GKGCMC	2 cut(s)	254, 767
BalI	TGGCCA	2 cut(s)	654, 1280
BanII	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
BbvI	GCAGC	3 cut(s)	565, 568, 2146
BceAI	ACGGC	7 cut(s)	569, 602, 608, 1071, 1578, 1816, 2217
BcgI	CGANNNNNNTGC	8 cut(s)	352, 386, 391, 425, 581, 615, 1202, 1236
BciT130I	CCWGG	1 cut(s)	632
BclI	TGATCA	3 cut(s)	102, 1348, 1759
BcnI	CCSGG	1 cut(s)	832
BcoDI	GTCTC	5 cut(s)	557, 1892, 2058, 2367, 2410
BfaI	CTAG	4 cut(s)	1229, 2150, 2219, 2229
BfmI	CTRYAG	2 cut(s)	1609, 2160
BfoI	RGCGCY	1 cut(s)	1501
BfuAI	ACCTGC	2 cut(s)	626, 745
BglI	GCCNNNNNGGC	4 cut(s)	345, 575, 710, 2330
BglII	AGATCT	1 cut(s)	2278
BisI	GCNGC	9 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710, 2160
BlpI	GCTNAGC	1 cut(s)	2355
BlsI	GCNGC	9 cut(s)	119, 577, 580, 583, 1019, 1357, 1693, 1711, 2161
Bme1390I	CCNGG	2 cut(s)	632, 832
Bme18I	GGWCC	7 cut(s)	194, 685, 1394, 1519, 1633, 1855, 2128
BmeRI	GACNNNNNGTC	1 cut(s)	946
BmeT110I	CYCGRG	2 cut(s)	1076, 1889
BmiI	GGNNCC	3 cut(s)	686, 2306, 2364
BmrFI	CCNGG	2 cut(s)	632, 832
BmsI	GCATC	4 cut(s)	38, 411, 531, 986
BpiI	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
BplI	GAGNNNNNCTC	4 cut(s)	319, 351, 474, 506
BpmI	CTGGAG	4 cut(s)	837, 2057, 2442, 2497
Bpu10I	CCTNAGC	1 cut(s)	326
Bpu1102I	GCTNAGC	1 cut(s)	2355
Bpu14I	TTCGAA	1 cut(s)	1438
BpuMI	CCSGG	1 cut(s)	832
BsaAI	YACGTR	2 cut(s)	1013, 1298
BsaBI	GATNNNNATC	2 cut(s)	925, 2277
BsaHI	GRCGYC	1 cut(s)	408
BsaI	GGTCTC	2 cut(s)	2058, 2410
BsaJI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BsaWI	WCCGGW	2 cut(s)	769, 1267
BsaXI	ACNNNNNCTCC	8 cut(s)	314, 344, 810, 840, 864, 894, 2234, 2264
Bsc4I	CCNNNNNNNGG	7 cut(s)	248, 747, 1298, 1630, 1693, 1993, 2401
Bse118I	RCCGGY	3 cut(s)	374, 759, 1291
Bse1I	ACTGG	3 cut(s)	820, 2459, 2514
Bse21I	CCTNAGG	1 cut(s)	1913
Bse3DI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
Bse8I	GATNNNNATC	2 cut(s)	925, 2277
BseBI	CCWGG	1 cut(s)	632
BseDI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BseGI	GGATG	2 cut(s)	1983, 2200
BseJI	GATNNNNATC	2 cut(s)	925, 2277
BseLI	CCNNNNNNNGG	7 cut(s)	248, 747, 1298, 1630, 1693, 1993, 2401
BseMI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
BseMII	CTCAG	6 cut(s)	317, 476, 566, 2160, 2205, 2346
BseNI	ACTGG	3 cut(s)	820, 2459, 2514
BseSI	GKGCMC	2 cut(s)	254, 767
BseXI	GCAGC	3 cut(s)	565, 568, 2146
BseYI	CCCAGC	4 cut(s)	1064, 1592, 1990, 2359
Bsh1236I	CGCG	1 cut(s)	1569
BsiHKCI	CYCGRG	2 cut(s)	1076, 1889
BslFI	GGGAC	2 cut(s)	1093, 2212
BslI	CCNNNNNNNGG	7 cut(s)	248, 747, 1298, 1630, 1693, 1993, 2401
BsmAI	GTCTC	5 cut(s)	557, 1892, 2058, 2367, 2410
BsmFI	GGGAC	2 cut(s)	1093, 2212
BsmI	GAATGC	1 cut(s)	1598
Bso31I	GGTCTC	2 cut(s)	2058, 2410
BsoBI	CYCGRG	2 cut(s)	1076, 1889
Bsp119I	TTCGAA	1 cut(s)	1438
Bsp1720I	GCTNAGC	1 cut(s)	2355
Bsp19I	CCATGG	1 cut(s)	570
BspCNI	CTCAG	6 cut(s)	318, 475, 565, 2161, 2206, 2347
BspFNI	CGCG	1 cut(s)	1569
BspHI	TCATGA	4 cut(s)	717, 837, 1143, 1762
BspLI	GGNNCC	3 cut(s)	686, 2306, 2364
BspMAI	CTGCAG	1 cut(s)	2164
BspMI	ACCTGC	2 cut(s)	626, 745
BspPI	GGATC	2 cut(s)	614, 724
BspQI	GCTCTTC	1 cut(s)	1507
BspT104I	TTCGAA	1 cut(s)	1438
BspTNI	GGTCTC	2 cut(s)	2058, 2410
BsrDI	GCAATG	4 cut(s)	162, 1101, 1745, 1795
BsrFI	RCCGGY	3 cut(s)	374, 759, 1291
BsrI	ACTGG	3 cut(s)	820, 2459, 2514
BssAI	RCCGGY	3 cut(s)	374, 759, 1291
BssECI	CCNNGG	7 cut(s)	570, 618, 624, 630, 1053, 1858, 1959
BssNI	GRCGYC	1 cut(s)	408
BssT1I	CCWWGG	2 cut(s)	570, 1959
Bst2UI	CCWGG	1 cut(s)	632
Bst4CI	ACNGT	6 cut(s)	543, 644, 909, 1633, 1859, 2304
Bst6I	CTCTTC	5 cut(s)	401, 1472, 1507, 1692, 1697
BstACI	GRCGYC	1 cut(s)	408
BstAPI	GCANNNNNTGC	1 cut(s)	1201
BstBAI	YACGTR	2 cut(s)	1013, 1298
BstBI	TTCGAA	1 cut(s)	1438
BstC8I	GCNNGC	8 cut(s)	376, 516, 520, 615, 757, 1060, 1567, 2352
BstDEI	CTNAG	9 cut(s)	313, 326, 462, 526, 552, 1913, 2169, 2214, 2355
BstDSI	CCRYGG	5 cut(s)	570, 618, 624, 1053, 1858
BstF5I	GGATG	2 cut(s)	1983, 2200
BstFNI	CGCG	1 cut(s)	1569
BstH2I	RGCGCY	1 cut(s)	1501
BstHHI	GCGC	3 cut(s)	1064, 1500, 1831
BstMAI	GTCTC	5 cut(s)	557, 1892, 2058, 2367, 2410
BstNI	CCWGG	1 cut(s)	632
BstNSI	RCATGY	2 cut(s)	1266, 1386
BstSCI	CCNGG	2 cut(s)	630, 830
BstSFI	CTRYAG	2 cut(s)	1609, 2160
BstSLI	GKGCMC	2 cut(s)	254, 767
BstUI	CGCG	1 cut(s)	1569
BstV1I	GCAGC	3 cut(s)	565, 568, 2146
BstV2I	GAAGAC	5 cut(s)	278, 412, 466, 874, 945
BstX2I	RGATCY	2 cut(s)	606, 2278
BstYI	RGATCY	2 cut(s)	606, 2278
Bsu36I	CCTNAGG	1 cut(s)	1913
BtgI	CCRYGG	5 cut(s)	570, 618, 624, 1053, 1858
BtgZI	GCGATG	1 cut(s)	1727
BtsCI	GGATG	2 cut(s)	1983, 2200
BtsI	GCAGTG	3 cut(s)	1851, 2169, 2272
BtsIMutI	CAGTG	7 cut(s)	276, 1851, 2169, 2211, 2272, 2466, 2521
BveI	ACCTGC	2 cut(s)	626, 745
Cac8I	GCNNGC	8 cut(s)	376, 516, 520, 615, 757, 1060, 1567, 2352
CaiI	CAGNNNCTG	1 cut(s)	2168
CciI	TCATGA	4 cut(s)	717, 837, 1143, 1762
CfoI	GCGC	3 cut(s)	1064, 1500, 1831
Cfr10I	RCCGGY	3 cut(s)	374, 759, 1291
CpoI	CGGWCCG	1 cut(s)	1633
CseI	GACGC	3 cut(s)	397, 1138, 2271
Csp6I	GTAC	2 cut(s)	2209, 2519
CspI	CGGWCCG	1 cut(s)	1633
CviQI	GTAC	2 cut(s)	2209, 2519
DdeI	CTNAG	9 cut(s)	313, 326, 462, 526, 552, 1913, 2169, 2214, 2355
DraI	TTTAAA	1 cut(s)	63
DraIII	CACNNNGTG	1 cut(s)	2401
DriI	GACNNNNNGTC	1 cut(s)	946
EaeI	YGGCCR	7 cut(s)	615, 621, 652, 828, 1113, 1278, 2392
Eam1104I	CTCTTC	5 cut(s)	401, 1472, 1507, 1692, 1697
Eam1105I	GACNNNNNGTC	1 cut(s)	946
EarI	CTCTTC	5 cut(s)	401, 1472, 1507, 1692, 1697
EciI	GGCGGA	1 cut(s)	1709
Ecl136II	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
Eco130I	CCWWGG	2 cut(s)	570, 1959
Eco147I	AGGCCT	1 cut(s)	1958
Eco24I	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
Eco31I	GGTCTC	2 cut(s)	2058, 2410
Eco47I	GGWCC	7 cut(s)	194, 685, 1394, 1519, 1633, 1855, 2128
Eco47III	AGCGCT	1 cut(s)	1499
Eco53kI	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
Eco57I	CTGAAG	1 cut(s)	183
Eco72I	CACGTG	1 cut(s)	1298
Eco81I	CCTNAGG	1 cut(s)	1913
Eco88I	CYCGRG	2 cut(s)	1076, 1889
EcoICRI	GAGCTC	5 cut(s)	319, 330, 535, 1168, 1369
EcoO109I	RGGNCCY	2 cut(s)	685, 2363
EcoRI	GAATTC	3 cut(s)	183, 855, 1374
EcoRII	CCWGG	1 cut(s)	630
EcoT14I	CCWWGG	2 cut(s)	570, 1959
EcoT22I	ATGCAT	1 cut(s)	680
EcoT38I	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
ErhI	CCWWGG	2 cut(s)	570, 1959
FaqI	GGGAC	2 cut(s)	1093, 2212
FauI	CCCGC	1 cut(s)	527
FbaI	TGATCA	3 cut(s)	102, 1348, 1759
Fnu4HI	GCNGC	9 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710, 2160
FokI	GGATG	2 cut(s)	1990, 2207
FriOI	GRGCYC	7 cut(s)	321, 332, 537, 668, 1170, 1371, 1531
Fsp4HI	GCNGC	9 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710, 2160
FspBI	CTAG	4 cut(s)	1229, 2150, 2219, 2229
GlaI	GCGC	3 cut(s)	1063, 1499, 1830
GluI	GCNGC	9 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710, 2160
GsaI	CCCAGC	4 cut(s)	1068, 1596, 1994, 2363
GsuI	CTGGAG	4 cut(s)	837, 2057, 2442, 2497
HaeII	RGCGCY	1 cut(s)	1501
HgaI	GACGC	3 cut(s)	397, 1138, 2271
HhaI	GCGC	3 cut(s)	1064, 1500, 1831
Hin1I	GRCGYC	1 cut(s)	408
Hin6I	GCGC	3 cut(s)	1062, 1498, 1829
HinP1I	GCGC	3 cut(s)	1062, 1498, 1829
HincII	GTYRAC	3 cut(s)	546, 589, 1807
HindII	GTYRAC	3 cut(s)	546, 589, 1807
HindIII	AAGCTT	2 cut(s)	1420, 1901
HpaI	GTTAAC	1 cut(s)	546
HphI	GGTGA	9 cut(s)	16, 490, 650, 1310, 1483, 1583, 1766, 2070, 2492
Hpy99I	CGWCG	3 cut(s)	113, 413, 596
HpyCH4III	ACNGT	6 cut(s)	543, 644, 909, 1633, 1859, 2304
HpyCH4IV	ACGT	4 cut(s)	594, 1012, 1082, 1297
HpyF3I	CTNAG	9 cut(s)	313, 326, 462, 526, 552, 1913, 2169, 2214, 2355
HpySE526I	ACGT	4 cut(s)	594, 1012, 1082, 1297
Hsp92I	GRCGYC	1 cut(s)	408
HspAI	GCGC	3 cut(s)	1062, 1498, 1829
KroI	GCCGGC	1 cut(s)	374
KroNI	GCCGGC	1 cut(s)	376
Ksp22I	TGATCA	3 cut(s)	102, 1348, 1759
KspAI	GTTAAC	1 cut(s)	546
LguI	GCTCTTC	1 cut(s)	1507
Lsp1109I	GCAGC	3 cut(s)	565, 568, 2146
LweI	GCATC	4 cut(s)	38, 411, 531, 986
MaeI	CTAG	4 cut(s)	1229, 2150, 2219, 2229
MaeII	ACGT	4 cut(s)	594, 1012, 1082, 1297
MaeIII	GTNAC	2 cut(s)	638, 1627
MfeI	CAATTG	4 cut(s)	432, 1616, 2101, 2256
MflI	RGATCY	2 cut(s)	606, 2278
MlsI	TGGCCA	2 cut(s)	654, 1280
MluNI	TGGCCA	2 cut(s)	654, 1280
MlyI	GAGTC	4 cut(s)	882, 1130, 1223, 1635
MmeI	TCCRAC	4 cut(s)	757, 1546, 1955, 2350
Mox20I	TGGCCA	2 cut(s)	654, 1280
Mph1103I	ATGCAT	1 cut(s)	680
MroNI	GCCGGC	1 cut(s)	374
MroXI	GAANNNNTTC	1 cut(s)	859
MscI	TGGCCA	2 cut(s)	654, 1280
MseI	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2069
Msp20I	TGGCCA	2 cut(s)	654, 1280
MspA1I	CMGCKG	2 cut(s)	1346, 1994
MspR9I	CCNGG	2 cut(s)	632, 832
MunI	CAATTG	4 cut(s)	432, 1616, 2101, 2256
Mva1269I	GAATGC	1 cut(s)	1598
MvaI	CCWGG	1 cut(s)	632
MvnI	CGCG	1 cut(s)	1569
NaeI	GCCGGC	1 cut(s)	376
NciI	CCSGG	1 cut(s)	832
NcoI	CCATGG	1 cut(s)	570
NgoMIV	GCCGGC	1 cut(s)	374
NlaIV	GGNNCC	3 cut(s)	686, 2306, 2364
NmeAIII	GCCGAG	1 cut(s)	1649
NmuCI	GTSAC	2 cut(s)	638, 1627
NsiI	ATGCAT	1 cut(s)	680
NspI	RCATGY	2 cut(s)	1266, 1386
NspV	TTCGAA	1 cut(s)	1438
PaeR7I	CTCGAG	1 cut(s)	1889
PagI	TCATGA	4 cut(s)	717, 837, 1143, 1762
PaqCI	CACCTGC	1 cut(s)	626
PceI	AGGCCT	1 cut(s)	1958
PciI	ACATGT	1 cut(s)	1262
PciSI	GCTCTTC	1 cut(s)	1507
PcsI	WCGNNNNNNNCGW	1 cut(s)	405
PctI	GAATGC	1 cut(s)	1598
PdiI	GCCGGC	1 cut(s)	376
PdmI	GAANNNNTTC	1 cut(s)	859
PfeI	GAWTC	7 cut(s)	266, 1072, 1108, 1302, 1430, 2422, 2507
PflMI	CCANNNNNTGG	2 cut(s)	747, 2401
PkrI	GCNGC	9 cut(s)	119, 577, 580, 583, 1019, 1357, 1693, 1711, 2161
PleI	GAGTC	4 cut(s)	882, 1129, 1222, 1634
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	4 cut(s)	882, 1129, 1222, 1634
Ppu21I	YACGTR	2 cut(s)	1013, 1298
PpuMI	RGGWCCY	1 cut(s)	685
PscI	ACATGT	1 cut(s)	1262
PshBI	ATTAAT	1 cut(s)	923
PsiI	TTATAA	1 cut(s)	1049
Psp124BI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
Psp5II	RGGWCCY	1 cut(s)	685
Psp6I	CCWGG	1 cut(s)	630
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	4 cut(s)	1064, 1592, 1990, 2359
PspGI	CCWGG	1 cut(s)	630
PspN4I	GGNNCC	3 cut(s)	686, 2306, 2364
PspPPI	RGGWCCY	1 cut(s)	685
PstI	CTGCAG	1 cut(s)	2164
PstNI	CAGNNNCTG	1 cut(s)	2168
PsuI	RGATCY	2 cut(s)	606, 2278
PvuII	CAGCTG	1 cut(s)	1346
RsaI	GTAC	2 cut(s)	2210, 2520
RsaNI	GTAC	2 cut(s)	2209, 2519
Rsr2I	CGGWCCG	1 cut(s)	1633
RsrII	CGGWCCG	1 cut(s)	1633
SacI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
SapI	GCTCTTC	1 cut(s)	1507
SaqAI	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2069
SatI	GCNGC	9 cut(s)	118, 576, 579, 582, 1018, 1356, 1692, 1710, 2160
SchI	GAGTC	4 cut(s)	882, 1130, 1223, 1635
ScrFI	CCNGG	2 cut(s)	632, 832
SfaNI	GCATC	4 cut(s)	38, 411, 531, 986
SfcI	CTRYAG	2 cut(s)	1609, 2160
Sfr274I	CTCGAG	1 cut(s)	1889
SfuI	TTCGAA	1 cut(s)	1438
SinI	GGWCC	7 cut(s)	194, 685, 1394, 1519, 1633, 1855, 2128
SlaI	CTCGAG	1 cut(s)	1889
SmlI	CTYRAG	1 cut(s)	1889
SmoI	CTYRAG	1 cut(s)	1889
SseBI	AGGCCT	1 cut(s)	1958
SspI	AATATT	2 cut(s)	261, 2236
SspMI	CTAG	4 cut(s)	1229, 2150, 2219, 2229
SstI	GAGCTC	5 cut(s)	321, 332, 537, 1170, 1371
StuI	AGGCCT	1 cut(s)	1958
StyD4I	CCNGG	2 cut(s)	630, 830
StyI	CCWWGG	2 cut(s)	570, 1959
TaaI	ACNGT	6 cut(s)	543, 644, 909, 1633, 1859, 2304
TaiI	ACGT	4 cut(s)	597, 1015, 1085, 1300
TaqI	TCGA	8 cut(s)	321, 929, 1438, 1531, 1558, 1890, 1951, 2338
TaqII	GACCGA	1 cut(s)	2145
TatI	WGTACW	2 cut(s)	2208, 2518
TauI	GCSGC	6 cut(s)	120, 578, 1020, 1358, 1694, 1712
TfiI	GAWTC	7 cut(s)	266, 1072, 1108, 1302, 1430, 2422, 2507
Tru1I	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2069
Tru9I	TTAA	9 cut(s)	62, 545, 806, 812, 923, 1424, 1554, 1998, 2069
TscAI	CASTG	7 cut(s)	276, 1851, 2169, 2218, 2272, 2466, 2521
TseFI	GTSAC	2 cut(s)	638, 1627
TseI	GCWGC	3 cut(s)	578, 581, 2159
Tsp45I	GTSAC	2 cut(s)	638, 1627
TspGWI	ACGGA	1 cut(s)	413
TspRI	CASTG	7 cut(s)	276, 1851, 2169, 2218, 2272, 2466, 2521
Van91I	CCANNNNNTGG	2 cut(s)	747, 2401
VneI	GTGCAC	1 cut(s)	763
VpaK11BI	GGWCC	7 cut(s)	194, 685, 1394, 1519, 1633, 1855, 2128
VspI	ATTAAT	1 cut(s)	923
XapI	RAATTY	3 cut(s)	183, 855, 1374
XceI	RCATGY	2 cut(s)	1266, 1386
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2122
XhoI	CTCGAG	1 cut(s)	1889
XmnI	GAANNNNTTC	1 cut(s)	859
XspI	CTAG	4 cut(s)	1229, 2150, 2219, 2229
Zsp2I	ATGCAT	1 cut(s)	680

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.