Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002613

Physical Location & Seq

Genomic Coordinates Forward (+)

20370205 .. 20374261

Gene Structure 4057 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G54980.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2556 bp

ATGAGATCTTTATTGGTTTTTAGAAAAATCCCTTCACGGATTCGTCTTCGTAACTTGAGAAACAATAAACCCTTTTGTTCACAGTCTCAGTTTCCCAAAGAATCTGAGAATCCAAGTCAAGAGCAGAGACTACTAGTTTACGGATCCACTTCTGAAGAAAACCCAGTTACCTCCAAAGTGAGTCTTTTGTCTGCGAAGCCTGAACAGAAGGACGATGCATCTGTAATCGATGTTCTTCTCAATCGCAGGAACAACCCAGAAGCTGCTCTTCGTTTCTATAATTGGGCTAGACCATGGCGTGGAAGTTTTGAAGATGGTGACGTCTTCTGGGTACTCATTCATATTCTGGTCAGTTCTCCTGAGACCTATGGACGTGCAAGTGATTTGCTCATACGGTACGTTTCTACGAGCAATCCAACTCCAATGGCAAGTGTTTTGGTCAGTAAATTGGTGGATTCGGCGAAATCATTTGGGTTTGAAGTCAATTCACGAGCTTTTAATTACTTATTGAATGCTTATTCTAAAGATAGGCAAACGGATCATGCGGTGGATATCGTCAACCAGATGTTGGAACTAGATGTGATTCCATTTTTTCCATATGTGAATAGGACCTTGAGTGCTTTAGTTCAAAGGAATTCGTTAACTGAAGCTAAGGAACTCTACAGTAGGATGGTTGCCATTGGTGTTGATGGTGATAATGTAACAACGCAATTGTTAATGAGAGCTAGTTTGAGAGAAGAAAAGCCTGCGGAGGCTTTGGAGGTTTTAAGTAGAGCTATTGAGAGAGGAGCAGAACCGGATAGTTTACTGTATAGTCTTGCTGTTCAGGCTTGTTGCAAGACGCTTGATTTGGCTATGGCTAATAGTTTATTGAGAGAGATGAAGGAGAAGAAGTTGTGTGTCCCGTCGCAGGAAACTTATACTTCTGTGATTTTGGCTTCTGTGAAGCAAGGTAATATGGATGATGCAATTAGATTGAAGGATGAGATGTTGAGTGATGGGATATCGATGAATGTAGTTGCTGCGACGAGTCTGATCACGGGACACTGCAAGAACAATGACTTGGTTAGTGCTTTGGTTTTGTTTGATAAGATGGAAAAGGAAGGCCCCTCTCCGAACAGCGTTACTTTTTCGGTTCTGATAGAATGGTTTCGTAAGAACGGGGAAATGGAAAAGGCGCTTGAATTTTACAAGAAAATGGAAGTCTTAGGTCTTACTCCTTCTGTCTTTCATGTCCACACGATAATTCAAGGGTGGTTGAAAGGTCAGAAACATGAAGAGGCACTGAAACTCTTTGACGAGTCTTTTGAAACCGGTCTTGCAAACGTGTTTGTATGTAACACTATATTATCCTGGTTATGCAAGCAAGGTAAGACAGACGAAGCTACAGAGTTATTAAGCAAGATGGAAAGTAGAGGTATTGGACCGAATGTAGTTTCTTACAATAACGTGATGCTTGGCCACTGCAGACAGAAAAACATGGACTTGGCTCGTATTGTTTTTTCAAATATACTGGAAAAGGGTCTAAAGCCGAATAACTACACGTATTCCATTCTGATTGATGGATGTTTCAGGAATCATGATGAACAAAACGCTTTGGAAGTCGTCAATCATATGACTTCTTCCAATATTGAAGTCAACGGGGTTGTATACCAAACAATTATCAATGGTTTATGCAAAGTTGGTCAGACATCCAAAGCAAGAGAATTGTTGGCAAATATGATCGAAGAAAAACGGTTATGTGTTAGTTGCATGAGTTACAATAGCATTATAGATGGATTTTTCAAAGAGGGTGAAATGGATTCTGCGGTTGCAGCATACGAAGAGATGTGTGGCAATGGTATTTCTCCAAACGTCATAACTTATACCAGCTTGATGAATGGGTTATGCAAAAATAACAGAATGGATCAAGCATTGGAAATGAGAGACGAAATGAAGAACAAAGGTGTCAAACTCGATATTCCAGCCTATGGTGCTCTTATCGATGGGTTCTGCAAAAGGAGTAACATGGAAAGCGCATCTGCTCTGTTCTCTGAACTCCTTGAAGAAGGATTGAATCCAAGCCAACCTATATACAACAGCTTGATTTCTGGATTTCGTAATCTGGGAAACATGGTAGCAGCCCTTGATTTGTACAAGAAAATGTTGAAAGACGGTCTACGATGTGATCTGGGTACATATACAACACTGATTGATGGATTGTTGAAAGATGGAAATTTGATCCTGGCCTCTGAGTTGTACACAGAAATGCAGGCGGTGGGTCTTGTCCCTGATGAAATCATTTACACAGTAATTGTAAACGGTCTTAGCAAGAAGGGGCAGTTTGTAAAAGTGGTTAAGATGTTTGAGGAGATGAAGAAGAATAATGTCACTCCGAATGTCCTTATCTACAACGCGGTGATTGCCGGACATTATAGAGAAGGAAATCTCGATGAGGCTTTTAGACTTCACGATGAGATGCTTGACAAGGGTATTTTACCCGATGGTGCTACATTTGATATTCTAGTTAGTGGGCAAGTCGGAAACCTCCAACCTGTACGTGCAGCAAGTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

851

Amino Acids

95.06

Weight (kDa)

5.66

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	251 - 340	3.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_long	PF17177	272 - 407	5.9e-09	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	302 - 351	1.4e-09	PPR repeat family
PPR_3	PF13812	325 - 384	5.4e-10	Pentatricopeptide repeat domain
PPR_2	PF13041	338 - 384	8e-13	PPR repeat family
PPR_3	PF13812	441 - 485	6.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	442 - 481	3.8e-11	PPR repeat family
PPR_2	PF13041	476 - 524	2.8e-16	PPR repeat family
PPR_1	PF12854	507 - 540	4.1e-06	PPR repeat
PPR_long	PF17177	514 - 661	5.8e-12	Pentacotripeptide-repeat region of PRORP
TPR_24	PF23276	573 - 679	1.5e-08	Fungal tetratrico peptide repeats
PPR_1	PF12854	580 - 610	5.3e-06	PPR repeat
PPR_3	PF13812	581 - 627	5.9e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	585 - 631	1.3e-16	PPR repeat family
PPR_long	PF17177	599 - 696	6.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	613 - 646	1.5e-13	PPR repeat
PPR_2	PF13041	617 - 666	5.3e-16	PPR repeat family
PPR	PF01535	620 - 650	1.1e-08	PPR repeat
PPR_3	PF13812	643 - 698	2e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	688 - 736	3.6e-11	PPR repeat family
PPR_long	PF17177	704 - 835	2.3e-10	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	712 - 767	6.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	725 - 771	9.1e-09	PPR repeat family
PPR_3	PF13812	748 - 801	6.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	757 - 794	5.1e-09	PPR repeat family
PPR	PF01535	761 - 790	7.3e-06	PPR repeat
PPR_3	PF13812	781 - 837	1.2e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	789 - 820	3.7e-07	PPR repeat
PPR_2	PF13041	792 - 838	5.7e-13	PPR repeat family
PPR	PF01535	796 - 825	1.3e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39230 AT3G54980 AT3G54980
fragaria_vesca	FvH4_5g25890 FvH4_5g25890 FvH4_5g25890
malus_domestica	MD04G1151000.v1.1 MD12G1164900.v1.1
prunus_persica	Prupe.6G275900_v2.0.a1 Prupe.6G275900_v2.0.a1
pyrus_communis	pycom04g13670 pycom12g15770
rosa_chinensis	RchiOBHm_Chr4g0410561 RchiOBHm_Chr4g0410571 RchiOBHm_Chr5g0071501 RchiOBHm_Chr6g0264781 RchiOBHm_Chr7g0216391 RchiOBHm_Chr7g0216401
rosa_laevigata	RLG00000002575 RLG00000002576 RLG00000002577 RLG00000031675
rosa_multiflora	Rmu_sc0000692.1_g000003 Rmu_sc0000692.1_g000005 Rmu_sc0006325.1_g000046
rosa_roxburghii	Rroxscaffold_159G00432870 Rroxscaffold_3G00243290 Rroxscaffold_4G00298080
rosa_rugosa	Rorug01G0081600 Rorug07G0160300
rosa_samantha	Rh2DG118300 Rh4CG174000 Rh4CG174100 Rh4CG174200 Rh7AG298700 Rh7AG298800 Rh7BG290300 Rh7BG290400
rosa_wichuraiana	Rw7G025370 Rw7G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	324
AccB7I	CCANNNNNTGG	3 cut(s)	299, 568, 1970
AccI	GTMKAC	2 cut(s)	1650, 2158
AccII	CGCG	1 cut(s)	2396
AciI	CCGC	5 cut(s)	545, 749, 1808, 2255, 2396
AclWI	GGATC	5 cut(s)	138, 151, 546, 1914, 2215
AcoI	YGGCCR	1 cut(s)	1459
AcsI	RAATTY	3 cut(s)	634, 1184, 2215
AcuI	CTGAAG	2 cut(s)	174, 666
AcyI	GRCGYC	1 cut(s)	321
AfaI	GTAC	6 cut(s)	333, 398, 2135, 2176, 2240, 2538
AfiI	CCNNNNNNNGG	4 cut(s)	299, 568, 910, 1970
AflIII	ACRYGT	2 cut(s)	1326, 1542
AgeI	ACCGGT	1 cut(s)	1313
AhlI	ACTAGT	1 cut(s)	133
AjiI	CACGTC	1 cut(s)	374
AjnI	CCWGG	2 cut(s)	1352, 2223
AjuI	GAANNNNNNNTTGG	2 cut(s)	1046, 1078
AluBI	AGCT	8 cut(s)	263, 494, 650, 725, 776, 1385, 1872, 2082
AluI	AGCT	8 cut(s)	263, 494, 650, 725, 776, 1385, 1872, 2082
Alw21I	GWGCWC	1 cut(s)	1978
Alw26I	GTCTC	4 cut(s)	90, 121, 356, 1920
AlwI	GGATC	5 cut(s)	138, 151, 546, 1914, 2215
AlwNI	CAGNNNCTG	1 cut(s)	263
AoxI	GGCC	3 cut(s)	1105, 1459, 2226
ApeKI	GCWGC	5 cut(s)	263, 1022, 1814, 2120, 2543
ApoI	RAATTY	3 cut(s)	634, 1184, 2215
ArsI	GACNNNNNNTTYG	4 cut(s)	832, 864, 1289, 1321
AsiGI	ACCGGT	1 cut(s)	1313
Asp700I	GAANNNNTTC	1 cut(s)	1149
AspLEI	GCGC	2 cut(s)	1180, 2018
AspS9I	GGNCC	3 cut(s)	609, 1106, 1424
AsuHPI	GGTGA	4 cut(s)	329, 704, 1805, 2410
AvaII	GGWCC	2 cut(s)	609, 1424
BalI	TGGCCA	1 cut(s)	1461
BamHI	GGATCC	1 cut(s)	143
BauI	CACGAG	1 cut(s)	489
BbsI	GAAGAC	2 cut(s)	38, 316
Bbv12I	GWGCWC	1 cut(s)	1978
BbvI	GCAGC	4 cut(s)	250, 1009, 1826, 2132
BciT130I	CCWGG	2 cut(s)	1354, 2225
BclI	TGATCA	1 cut(s)	1035
BcoDI	GTCTC	4 cut(s)	90, 121, 356, 1920
BcuI	ACTAGT	1 cut(s)	133
BfaI	CTAG	5 cut(s)	134, 288, 575, 726, 2504
BfmI	CTRYAG	3 cut(s)	661, 1386, 1465
BfoI	RGCGCY	1 cut(s)	1181
BglII	AGATCT	1 cut(s)	5
BisI	GCNGC	5 cut(s)	264, 1023, 1815, 2121, 2544
BlsI	GCNGC	5 cut(s)	265, 1024, 1816, 2122, 2545
Bme1390I	CCNGG	2 cut(s)	1354, 2225
Bme18I	GGWCC	2 cut(s)	609, 1424
BmgBI	CACGTC	1 cut(s)	374
BmgT120I	GGNCC	3 cut(s)	609, 1106, 1424
BmiI	GGNNCC	2 cut(s)	145, 1108
BmrFI	CCNGG	2 cut(s)	1354, 2225
BmrI	ACTGGG	1 cut(s)	158
BmsI	GCATC	6 cut(s)	205, 227, 955, 1443, 2027, 2448
BmuI	ACTGGG	1 cut(s)	158
BpiI	GAAGAC	2 cut(s)	38, 316
Bpu10I	CCTNAGC	1 cut(s)	651
BpuEI	CTTGAG	2 cut(s)	76, 634
Bsa29I	ATCGAT	3 cut(s)	228, 1007, 1983
BsaAI	YACGTR	2 cut(s)	1545, 2540
BsaHI	GRCGYC	1 cut(s)	321
BsaI	GGTCTC	1 cut(s)	356
BsaJI	CCNNGG	1 cut(s)	293
BsaWI	WCCGGW	2 cut(s)	796, 1313
BsaXI	ACNNNNNCTCC	2 cut(s)	878, 908
Bsc4I	CCNNNNNNNGG	4 cut(s)	299, 568, 910, 1970
Bse118I	RCCGGY	1 cut(s)	1313
Bse1I	ACTGG	2 cut(s)	164, 1518
Bse3DI	GCAATG	1 cut(s)	1843
BseBI	CCWGG	2 cut(s)	1354, 2225
BseCI	ATCGAT	3 cut(s)	228, 1007, 1983
BseDI	CCNNGG	1 cut(s)	293
BseGI	GGATG	5 cut(s)	675, 967, 988, 1571, 1691
BseLI	CCNNNNNNNGG	4 cut(s)	299, 568, 910, 1970
BseMI	GCAATG	1 cut(s)	1843
BseMII	CTCAG	4 cut(s)	96, 101, 351, 2223
BseNI	ACTGG	2 cut(s)	164, 1518
BseRI	GAGGAG	2 cut(s)	801, 2363
BseXI	GCAGC	4 cut(s)	250, 1009, 1826, 2132
Bsh1236I	CGCG	1 cut(s)	2396
BshFI	GGCC	3 cut(s)	1107, 1461, 2228
BshTI	ACCGGT	1 cut(s)	1313
BshVI	ATCGAT	3 cut(s)	228, 1007, 1983
BsiHKAI	GWGCWC	1 cut(s)	1978
BsiSI	CCGG	3 cut(s)	797, 1314, 2406
BslFI	GGGAC	3 cut(s)	887, 1056, 2252
BslI	CCNNNNNNNGG	4 cut(s)	299, 568, 910, 1970
BsmAI	GTCTC	4 cut(s)	90, 121, 356, 1920
BsmBI	CGTCTC	1 cut(s)	1920
BsmFI	GGGAC	3 cut(s)	887, 1056, 2252
BsmI	GAATGC	1 cut(s)	517
BsnI	GGCC	3 cut(s)	1107, 1461, 2228
Bso31I	GGTCTC	1 cut(s)	356
Bsp1286I	GDGCHC	1 cut(s)	1978
Bsp1407I	TGTACA	2 cut(s)	2133, 2238
Bsp143I	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
Bsp19I	CCATGG	1 cut(s)	293
BspACI	CCGC	5 cut(s)	545, 749, 1808, 2255, 2396
BspANI	GGCC	3 cut(s)	1107, 1461, 2228
BspCNI	CTCAG	4 cut(s)	97, 100, 352, 2224
BspDI	ATCGAT	3 cut(s)	228, 1007, 1983
BspFNI	CGCG	1 cut(s)	2396
BspHI	TCATGA	1 cut(s)	1579
BspLI	GGNNCC	2 cut(s)	145, 1108
BspMAI	CTGCAG	1 cut(s)	1469
BspPI	GGATC	5 cut(s)	138, 151, 546, 1914, 2215
BspQI	GCTCTTC	1 cut(s)	273
BspTNI	GGTCTC	1 cut(s)	356
BsrDI	GCAATG	1 cut(s)	1843
BsrFI	RCCGGY	1 cut(s)	1313
BsrGI	TGTACA	2 cut(s)	2133, 2238
BsrI	ACTGG	2 cut(s)	164, 1518
BssAI	RCCGGY	1 cut(s)	1313
BssECI	CCNNGG	1 cut(s)	293
BssMI	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
BssNAI	GTATAC	1 cut(s)	1651
BssNI	GRCGYC	1 cut(s)	321
BssSI	CACGAG	1 cut(s)	489
BssT1I	CCWWGG	1 cut(s)	293
Bst1107I	GTATAC	1 cut(s)	1651
Bst2BI	CACGAG	1 cut(s)	489
Bst2UI	CCWGG	2 cut(s)	1354, 2225
Bst4CI	ACNGT	8 cut(s)	84, 396, 665, 810, 1737, 2156, 2290, 2303
Bst6I	CTCTTC	3 cut(s)	273, 1272, 1818
BstACI	GRCGYC	1 cut(s)	321
BstAUI	TGTACA	2 cut(s)	2133, 2238
BstBAI	YACGTR	2 cut(s)	1545, 2540
BstC8I	GCNNGC	3 cut(s)	747, 1364, 2253
BstDEI	CTNAG	7 cut(s)	87, 105, 360, 651, 1207, 2232, 2306
BstDSI	CCRYGG	1 cut(s)	293
BstF5I	GGATG	5 cut(s)	675, 967, 988, 1571, 1691
BstFNI	CGCG	1 cut(s)	2396
BstH2I	RGCGCY	1 cut(s)	1181
BstHHI	GCGC	2 cut(s)	1180, 2018
BstKTI	GATC	8 cut(s)	8, 146, 541, 1038, 1725, 1909, 2170, 2223
BstMAI	GTCTC	4 cut(s)	90, 121, 356, 1920
BstMBI	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
BstMWI	GCNNNNNNNGC	4 cut(s)	827, 1814, 1973, 2402
BstNI	CCWGG	2 cut(s)	1354, 2225
BstSCI	CCNGG	2 cut(s)	1352, 2223
BstSFI	CTRYAG	3 cut(s)	661, 1386, 1465
BstUI	CGCG	1 cut(s)	2396
BstV1I	GCAGC	4 cut(s)	250, 1009, 1826, 2132
BstV2I	GAAGAC	2 cut(s)	38, 316
BstX2I	RGATCY	2 cut(s)	5, 143
BstYI	RGATCY	2 cut(s)	5, 143
BstZ17I	GTATAC	1 cut(s)	1651
Bsu15I	ATCGAT	3 cut(s)	228, 1007, 1983
BsuRI	GGCC	3 cut(s)	1107, 1461, 2228
BsuTUI	ATCGAT	3 cut(s)	228, 1007, 1983
BtgI	CCRYGG	1 cut(s)	293
BtrI	CACGTC	1 cut(s)	374
BtsCI	GGATG	5 cut(s)	675, 967, 988, 1571, 1691
BtsI	GCAGTG	2 cut(s)	1045, 1462
BtsIMutI	CAGTG	4 cut(s)	1045, 1283, 1462, 2186
Cac8I	GCNNGC	3 cut(s)	747, 1364, 2253
CaiI	CAGNNNCTG	1 cut(s)	263
CciI	TCATGA	1 cut(s)	1579
CfoI	GCGC	2 cut(s)	1180, 2018
Cfr10I	RCCGGY	1 cut(s)	1313
Cfr13I	GGNCC	3 cut(s)	609, 1106, 1424
ClaI	ATCGAT	3 cut(s)	228, 1007, 1983
CseI	GACGC	1 cut(s)	850
Csp6I	GTAC	6 cut(s)	332, 397, 2134, 2175, 2239, 2537
CspAI	ACCGGT	1 cut(s)	1313
CviAII	CATG	9 cut(s)	294, 542, 1232, 1274, 1480, 1580, 1753, 2008, 2113
CviQI	GTAC	6 cut(s)	332, 397, 2134, 2175, 2239, 2537
DdeI	CTNAG	7 cut(s)	87, 105, 360, 651, 1207, 2232, 2306
DpnI	GATC	8 cut(s)	7, 145, 540, 1037, 1724, 1908, 2169, 2222
DpnII	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
EaeI	YGGCCR	1 cut(s)	1459
Eam1104I	CTCTTC	3 cut(s)	273, 1272, 1818
EarI	CTCTTC	3 cut(s)	273, 1272, 1818
Eco130I	CCWWGG	1 cut(s)	293
Eco31I	GGTCTC	1 cut(s)	356
Eco32I	GATATC	2 cut(s)	553, 1005
Eco47I	GGWCC	2 cut(s)	609, 1424
Eco57I	CTGAAG	2 cut(s)	174, 666
EcoO109I	RGGNCCY	2 cut(s)	609, 1106
EcoRI	GAATTC	1 cut(s)	634
EcoRII	CCWGG	2 cut(s)	1352, 2223
EcoRV	GATATC	2 cut(s)	553, 1005
EcoT14I	CCWWGG	1 cut(s)	293
EcoT22I	ATGCAT	1 cut(s)	220
ErhI	CCWWGG	1 cut(s)	293
Esp3I	CGTCTC	1 cut(s)	1920
FaeI	CATG	9 cut(s)	297, 545, 1235, 1277, 1483, 1583, 1756, 2011, 2116
FalI	AAGNNNNNCTT	4 cut(s)	168, 200, 252, 284
FaqI	GGGAC	3 cut(s)	887, 1056, 2252
FatI	CATG	9 cut(s)	293, 541, 1231, 1273, 1479, 1579, 1752, 2007, 2112
FauNDI	CATATG	2 cut(s)	598, 1614
FbaI	TGATCA	1 cut(s)	1035
FblI	GTMKAC	2 cut(s)	1650, 2158
Fnu4HI	GCNGC	5 cut(s)	264, 1023, 1815, 2121, 2544
FokI	GGATG	5 cut(s)	682, 974, 995, 1578, 1678
Fsp4HI	GCNGC	5 cut(s)	264, 1023, 1815, 2121, 2544
FspBI	CTAG	5 cut(s)	134, 288, 575, 726, 2504
GlaI	GCGC	2 cut(s)	1179, 2017
GluI	GCNGC	5 cut(s)	264, 1023, 1815, 2121, 2544
HaeII	RGCGCY	1 cut(s)	1181
HaeIII	GGCC	3 cut(s)	1107, 1461, 2228
HapII	CCGG	3 cut(s)	797, 1314, 2406
HgaI	GACGC	1 cut(s)	850
HhaI	GCGC	2 cut(s)	1180, 2018
Hin1I	GRCGYC	1 cut(s)	321
Hin1II	CATG	9 cut(s)	297, 545, 1235, 1277, 1483, 1583, 1756, 2011, 2116
Hin6I	GCGC	2 cut(s)	1178, 2016
HinP1I	GCGC	2 cut(s)	1178, 2016
HincII	GTYRAC	3 cut(s)	559, 642, 1639
HindII	GTYRAC	3 cut(s)	559, 642, 1639
HpaI	GTTAAC	1 cut(s)	642
HpaII	CCGG	3 cut(s)	797, 1314, 2406
HphI	GGTGA	4 cut(s)	329, 704, 1805, 2410
Hpy188III	TCNNGA	8 cut(s)	119, 359, 489, 1573, 1580, 2091, 2429, 2450
Hpy99I	CGWCG	2 cut(s)	910, 1030
HpyAV	CCTTC	9 cut(s)	42, 202, 877, 973, 1097, 1230, 2042, 2308, 2414
HpyCH4III	ACNGT	8 cut(s)	84, 396, 665, 810, 1737, 2156, 2290, 2303
HpyCH4IV	ACGT	8 cut(s)	321, 373, 399, 1326, 1449, 1544, 1854, 2539
HpyF10VI	GCNNNNNNNGC	4 cut(s)	827, 1814, 1973, 2402
HpyF3I	CTNAG	7 cut(s)	87, 105, 360, 651, 1207, 2232, 2306
HpySE526I	ACGT	8 cut(s)	321, 373, 399, 1326, 1449, 1544, 1854, 2539
Hsp92I	GRCGYC	1 cut(s)	321
Hsp92II	CATG	9 cut(s)	297, 545, 1235, 1277, 1483, 1583, 1756, 2011, 2116
HspAI	GCGC	2 cut(s)	1178, 2016
Ksp22I	TGATCA	1 cut(s)	1035
KspAI	GTTAAC	1 cut(s)	642
Kzo9I	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
LguI	GCTCTTC	1 cut(s)	273
LmnI	GCTCC	1 cut(s)	788
Lsp1109I	GCAGC	4 cut(s)	250, 1009, 1826, 2132
LweI	GCATC	6 cut(s)	205, 227, 955, 1443, 2027, 2448
MaeI	CTAG	5 cut(s)	134, 288, 575, 726, 2504
MaeII	ACGT	8 cut(s)	321, 373, 399, 1326, 1449, 1544, 1854, 2539
MaeIII	GTNAC	9 cut(s)	50, 166, 317, 700, 1123, 1337, 1757, 2003, 2368
MalI	GATC	8 cut(s)	7, 145, 540, 1037, 1724, 1908, 2169, 2222
MboI	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
MfeI	CAATTG	1 cut(s)	710
MflI	RGATCY	2 cut(s)	5, 143
MhlI	GDGCHC	1 cut(s)	1978
MlsI	TGGCCA	1 cut(s)	1461
MluNI	TGGCCA	1 cut(s)	1461
MlyI	GAGTC	3 cut(s)	190, 1039, 1310
MmeI	TCCRAC	4 cut(s)	440, 549, 2500, 2554
Mox20I	TGGCCA	1 cut(s)	1461
Mph1103I	ATGCAT	1 cut(s)	220
MroXI	GAANNNNTTC	1 cut(s)	1149
MscI	TGGCCA	1 cut(s)	1461
MseI	TTAA	6 cut(s)	498, 641, 716, 767, 1397, 2337
MslI	CAYNNNNRTG	1 cut(s)	2246
Msp20I	TGGCCA	1 cut(s)	1461
MspI	CCGG	3 cut(s)	797, 1314, 2406
MspR9I	CCNGG	2 cut(s)	1354, 2225
MunI	CAATTG	1 cut(s)	710
Mva1269I	GAATGC	1 cut(s)	517
MvaI	CCWGG	2 cut(s)	1354, 2225
MvnI	CGCG	1 cut(s)	2396
MwoI	GCNNNNNNNGC	4 cut(s)	827, 1814, 1973, 2402
NcoI	CCATGG	1 cut(s)	293
NdeI	CATATG	2 cut(s)	598, 1614
NdeII	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
NlaIII	CATG	9 cut(s)	297, 545, 1235, 1277, 1483, 1583, 1756, 2011, 2116
NlaIV	GGNNCC	2 cut(s)	145, 1108
NmuCI	GTSAC	2 cut(s)	317, 2368
NsiI	ATGCAT	1 cut(s)	220
PagI	TCATGA	1 cut(s)	1579
PciSI	GCTCTTC	1 cut(s)	273
PctI	GAATGC	1 cut(s)	517
PdmI	GAANNNNTTC	1 cut(s)	1149
PfeI	GAWTC	8 cut(s)	40, 101, 109, 455, 583, 1576, 1802, 2056
PflMI	CCANNNNNTGG	3 cut(s)	299, 568, 1970
PinAI	ACCGGT	1 cut(s)	1313
PkrI	GCNGC	5 cut(s)	265, 1024, 1816, 2122, 2545
PleI	GAGTC	3 cut(s)	189, 1038, 1309
PpsI	GAGTC	3 cut(s)	189, 1038, 1309
Ppu21I	YACGTR	2 cut(s)	1545, 2540
PpuMI	RGGWCCY	1 cut(s)	609
Psp5II	RGGWCCY	1 cut(s)	609
Psp6I	CCWGG	2 cut(s)	1352, 2223
PspGI	CCWGG	2 cut(s)	1352, 2223
PspN4I	GGNNCC	2 cut(s)	145, 1108
PspPI	GGNCC	3 cut(s)	609, 1106, 1424
PspPPI	RGGWCCY	1 cut(s)	609
PsrI	GAACNNNNNNTAC	2 cut(s)	216, 248
PstI	CTGCAG	1 cut(s)	1469
PstNI	CAGNNNCTG	1 cut(s)	263
PsuI	RGATCY	2 cut(s)	5, 143
RsaI	GTAC	6 cut(s)	333, 398, 2135, 2176, 2240, 2538
RsaNI	GTAC	6 cut(s)	332, 397, 2134, 2175, 2239, 2537
RseI	CAYNNNNRTG	1 cut(s)	2246
SapI	GCTCTTC	1 cut(s)	273
SaqAI	TTAA	6 cut(s)	498, 641, 716, 767, 1397, 2337
SatI	GCNGC	5 cut(s)	264, 1023, 1815, 2121, 2544
Sau3AI	GATC	8 cut(s)	5, 143, 538, 1035, 1722, 1906, 2167, 2220
Sau96I	GGNCC	3 cut(s)	609, 1106, 1424
SchI	GAGTC	3 cut(s)	190, 1039, 1310
ScrFI	CCNGG	2 cut(s)	1354, 2225
SduI	GDGCHC	1 cut(s)	1978
SfaNI	GCATC	6 cut(s)	205, 227, 955, 1443, 2027, 2448
SfcI	CTRYAG	3 cut(s)	661, 1386, 1465
SinI	GGWCC	2 cut(s)	609, 1424
SmiMI	CAYNNNNRTG	1 cut(s)	2246
SmlI	CTYRAG	2 cut(s)	55, 613
SmoI	CTYRAG	2 cut(s)	55, 613
SpeI	ACTAGT	1 cut(s)	133
SsiI	CCGC	5 cut(s)	545, 749, 1808, 2255, 2396
SspI	AATATT	1 cut(s)	1630
SspMI	CTAG	5 cut(s)	134, 288, 575, 726, 2504
StyD4I	CCNGG	2 cut(s)	1352, 2223
StyI	CCWWGG	1 cut(s)	293
TaaI	ACNGT	8 cut(s)	84, 396, 665, 810, 1737, 2156, 2290, 2303
TaiI	ACGT	8 cut(s)	324, 376, 402, 1329, 1452, 1547, 1857, 2542
TaqI	TCGA	6 cut(s)	228, 1007, 1725, 1956, 1983, 2430
TaqII	GACCGA	1 cut(s)	1441
TatI	WGTACW	2 cut(s)	2133, 2238
TfiI	GAWTC	8 cut(s)	40, 101, 109, 455, 583, 1576, 1802, 2056
Tru1I	TTAA	6 cut(s)	498, 641, 716, 767, 1397, 2337
Tru9I	TTAA	6 cut(s)	498, 641, 716, 767, 1397, 2337
TscAI	CASTG	4 cut(s)	1052, 1290, 1469, 2193
TseFI	GTSAC	2 cut(s)	317, 2368
TseI	GCWGC	5 cut(s)	263, 1022, 1814, 2120, 2543
Tsp45I	GTSAC	2 cut(s)	317, 2368
TspGWI	ACGGA	3 cut(s)	52, 156, 551
TspRI	CASTG	4 cut(s)	1052, 1290, 1469, 2193
Van91I	CCANNNNNTGG	3 cut(s)	299, 568, 1970
VpaK11BI	GGWCC	2 cut(s)	609, 1424
XapI	RAATTY	3 cut(s)	634, 1184, 2215
XmiI	GTMKAC	2 cut(s)	1650, 2158
XmnI	GAANNNNTTC	1 cut(s)	1149
XspI	CTAG	5 cut(s)	134, 288, 575, 726, 2504
ZraI	GACGTC	1 cut(s)	322
Zsp2I	ATGCAT	1 cut(s)	220

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.