Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0002613

Physical Location & Seq

Genomic Coordinates Reverse (-)

24620657 .. 24622851

Gene Structure 2195 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1164900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1842 bp

ATGGGCAATCCGAAGACTCACAGGCGTGCTAAGATGTTGCTTAACCAGTACGTTTCCGGCAATTCGGGCGTCTTTGTCCATCACTTGGTGGACTGCGCCAAACGGTTTGATTTTGAACTAGAATCTCAGGTTTTTGGCTATTTATTGAACAATTATGTTAGAGCCAATCGAATCGAATGTGCCATTGATTGCTTTAGTAGAACTCTGGAGCTTGAGATGTGTCCTTGTGTTACGTATGTAAATATTCTTTTAACAGAATTGGTTAGGAGAAATATGATGGGGGAAGCACGAGAGGTGTATGATAAAATGGCTTTGAGAGGAATTGGAGGTGATCGTGTCACTCTACACGTGATGATGCGTGGTTGTTTGAAGCAGGGGAAGCCGGATGACGCAGAGGAGTACTTTCGAGAGGCGAGGGCAAGAGAGGTAGAACTTGACGCAGCATCCTATAGTGTTGCTATTGAAGCTTTTTGTAAGAAACCCAATTCGTGTTTGGCTTTGGAGTTGTTGGATGAAATGAGAGAAATGGGGTGGGTTCCTTCCGTGGTCACGTTTAGCAGTGTCATCACGGCTTGTGTGAAGCAGAGGAATATGGTTGAAGCCATAAGGATTAAGGATGGTATGGTAAGTTGTGGGTACCCAGTTAATTTGGTTGCTGCTACAAGCTTGATGAAGGGTTATTGTGTGCAAGGGAATTTGGAGAGTGCTTTGGGTTTGTTCATTATGATTATAGAGGATGGACTTACTCCAAACAGGGTTACGTATGCAATTCTTATAGAATACTGCTGTAAGAATGGGAACATGGAAAAGGCTTATGAGCTTTACACGCAAATGAAAGACATGGATATTTTTCCTGATGTCTTCATTGGTAAAGTAAGTGAAGCTTGTAGTTTATGGGATAAAATGATGTGTAAAGGTGTGGTACCTAATGTAGTATCTTACAATAGCAAGATAAATGGCTATTGCAGAATAGGGAATATGGAGCATGCACGCAGTGTATTTGTGGAGATGCTTAAAAGGGGTTTGAAACCAAACGATCATGTCTTTGATGATATGGTGGCTGCCAAGATTTCTCCCACAGGCTTCACAGTTGGCATTGTCATCGATGGCTTGTGCAAAGCTGGTCGTACATCCGAGGCAAGTGATATGTTAAAGAAAATTGTTGCGAGGGGTTTTATTCCTGGATGTGTTAGTTACAATAGCATTATAAATGGGTATACCAAGGAGGGTGACATTAATTCTGCACTGGCTGTATACAGAGAAATGTGTGAAGACATGGGATCTGCGCGTGAACTTTTCTTCGAACTCCTTCAAGTTGGTTTATCTCCAAATGTAGCTGTTTACAGTAGCATGATATCTGGCTTCCGGAGTTTAAATAACGTGGAAGCGGCTCTTGACTGGCACAACAGGATGATTAGAGAAGGTATTCCTTGTGATTTGAAAGCATACACTACATTAATTGATGGATTTCTAAGAGAGGGAGAGTTACAATCTGCTACAGATCTCTACTCAGAGATGCTTCAGAAGGGAATTGTACCTGATAAAAGAACATATACAGTTCTGATAAATGCCCTTCGTAACAAAGGACAGCTAGAGAATGCACGAAAGAACTTTGAAGACATGAATAGTAGGGGAATGACTCCCAGTGTTCATATTTATAATACTTTGGTTGCTGGGAACTTCAAGGAGGGGCATTTGCAAGAGGCTTTTAGGTTGCATGATGAGATGCTTGACAAAGGTCTTGTACCACATGACGATACGTATGATACTCTTGTAACTGGGAAGTTCCAGGGAACGAACGGTCTTGTTGGAACTTCATGTGGAGATATGCACCAGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

614

Amino Acids

68.6

Weight (kDa)

5.93

Isoelectric Point (pI)

30.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	75 - 124	1.4e-06	PPR repeat family
PPR_2	PF13041	113 - 159	5.6e-07	PPR repeat family
TPR_24	PF23276	123 - 240	5.8e-08	Fungal tetratrico peptide repeats
PPR_3	PF13812	135 - 194	1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	154 - 194	1.6e-06	PPR repeat family
PPR_2	PF13041	180 - 228	3.8e-07	PPR repeat family
PPR_3	PF13812	203 - 265	1.4e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	216 - 264	6.5e-10	PPR repeat family
PPR_1	PF12854	246 - 279	6.8e-10	PPR repeat
PPR_2	PF13041	253 - 288	1.2e-09	PPR repeat family
PPR	PF01535	253 - 282	5.3e-06	PPR repeat
PPR_2	PF13041	290 - 322	2.5e-07	PPR repeat family
PPR_3	PF13812	298 - 345	3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	305 - 337	3.1e-09	PPR repeat
PPR_2	PF13041	309 - 347	5.5e-12	PPR repeat family
PPR	PF01535	312 - 342	4.7e-07	PPR repeat
PPR_1	PF12854	390 - 422	3.3e-07	PPR repeat
PPR_2	PF13041	394 - 424	1.2e-06	PPR repeat family
PPR	PF01535	397 - 424	8.2e-07	PPR repeat
PPR_2	PF13041	443 - 491	2e-09	PPR repeat family
PPR_3	PF13812	471 - 525	2.7e-06	Pentatricopeptide repeat domain
TPR_24	PF23276	471 - 575	5e-08	Fungal tetratrico peptide repeats
PPR	PF01535	482 - 511	7.6e-06	PPR repeat
PPR_2	PF13041	482 - 525	1.2e-14	PPR repeat family
PPR_1	PF12854	544 - 576	1e-06	PPR repeat
PPR_2	PF13041	548 - 594	2.5e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39230 AT3G54980 AT3G54980
fragaria_vesca	FvH4_5g25890 FvH4_5g25890 FvH4_5g25890
malus_domestica	MD04G1151000.v1.1 MD12G1164900.v1.1
prunus_persica	Prupe.6G275900_v2.0.a1 Prupe.6G275900_v2.0.a1
pyrus_communis	pycom04g13670 pycom12g15770
rosa_chinensis	RchiOBHm_Chr4g0410561 RchiOBHm_Chr4g0410571 RchiOBHm_Chr5g0071501 RchiOBHm_Chr6g0264781 RchiOBHm_Chr7g0216391 RchiOBHm_Chr7g0216401
rosa_laevigata	RLG00000002575 RLG00000002576 RLG00000002577 RLG00000031675
rosa_multiflora	Rmu_sc0000692.1_g000003 Rmu_sc0000692.1_g000005 Rmu_sc0006325.1_g000046
rosa_roxburghii	Rroxscaffold_159G00432870 Rroxscaffold_3G00243290 Rroxscaffold_4G00298080
rosa_rugosa	Rorug01G0081600 Rorug07G0160300
rosa_samantha	Rh2DG118300 Rh4CG174000 Rh4CG174100 Rh4CG174200 Rh7AG298700 Rh7AG298800 Rh7BG290300 Rh7BG290400
rosa_wichuraiana	Rw7G025370 Rw7G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1208, 1659
Acc65I	GGTACC	2 cut(s)	636, 922
AccB1I	GGYRCC	2 cut(s)	636, 922
AccB7I	CCANNNNNTGG	1 cut(s)	85
AccI	GTMKAC	2 cut(s)	1217, 1254
AccII	CGCG	1 cut(s)	1288
AccIII	TCCGGA	1 cut(s)	1365
AciI	CCGC	1 cut(s)	1388
AclWI	GGATC	1 cut(s)	1288
AcsI	RAATTY	1 cut(s)	694
AcuI	CTGAAG	1 cut(s)	1505
AcvI	CACGTG	1 cut(s)	349
AcyI	GRCGYC	1 cut(s)	69
AdeI	CACNNNGTG	2 cut(s)	88, 995
AfaI	GTAC	7 cut(s)	50, 401, 638, 924, 1129, 1536, 1746
AfiI	CCNNNNNNNGG	1 cut(s)	85
AflIII	ACRYGT	1 cut(s)	346
AjnI	CCWGG	2 cut(s)	1180, 1788
AleI	CACNNNNGTG	1 cut(s)	24
AluBI	AGCT	8 cut(s)	211, 467, 666, 820, 884, 1121, 1337, 1591
AluI	AGCT	8 cut(s)	211, 467, 666, 820, 884, 1121, 1337, 1591
AlwI	GGATC	1 cut(s)	1288
Aor13HI	TCCGGA	1 cut(s)	1365
ApeKI	GCWGC	3 cut(s)	440, 656, 1061
ApoI	RAATTY	1 cut(s)	694
AseI	ATTAAT	2 cut(s)	1236, 1457
Asp700I	GAANNNNTTC	2 cut(s)	1308, 1425
Asp718I	GGTACC	2 cut(s)	636, 922
AspLEI	GCGC	2 cut(s)	98, 1288
AsuHPI	GGTGA	2 cut(s)	341, 1241
AsuII	TTCGAA	1 cut(s)	1302
BaeI	ACNNNNGTAYC	2 cut(s)	1758, 1791
BanI	GGYRCC	2 cut(s)	636, 922
BauI	CACGAG	1 cut(s)	288
BbrPI	CACGTG	1 cut(s)	349
BbsI	GAAGAC	4 cut(s)	20, 853, 1278, 1623
BbvI	GCAGC	3 cut(s)	452, 643, 1048
BccI	CCATC	6 cut(s)	87, 271, 611, 731, 1100, 1457
BceAI	ACGGC	1 cut(s)	585
BcgI	CGANNNNNNTGC	2 cut(s)	1084, 1118
BciT130I	CCWGG	2 cut(s)	1182, 1790
BfaI	CTAG	2 cut(s)	119, 1592
BfmI	CTRYAG	2 cut(s)	448, 1497
BglII	AGATCT	1 cut(s)	1501
BisI	GCNGC	4 cut(s)	441, 657, 1062, 1389
BlsI	GCNGC	4 cut(s)	442, 658, 1063, 1390
BmcAI	AGTACT	1 cut(s)	401
Bme1390I	CCNGG	2 cut(s)	1182, 1790
BmiI	GGNNCC	3 cut(s)	537, 638, 924
BmrFI	CCNGG	2 cut(s)	1182, 1790
BmrI	ACTGGG	3 cut(s)	635, 1638, 1788
BmsI	GCATC	5 cut(s)	345, 452, 999, 1506, 1716
BmuI	ACTGGG	3 cut(s)	635, 1638, 1788
BoxI	GACNNNNGTC	1 cut(s)	1737
BpiI	GAAGAC	4 cut(s)	20, 853, 1278, 1623
BpmI	CTGGAG	1 cut(s)	227
Bpu14I	TTCGAA	1 cut(s)	1302
BpuEI	CTTGAG	1 cut(s)	233
Bsa29I	ATCGAT	1 cut(s)	1104
BsaAI	YACGTR	4 cut(s)	234, 349, 762, 1761
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	4 cut(s)	543, 1134, 1221, 1789
BsaWI	WCCGGW	1 cut(s)	1365
Bsc4I	CCNNNNNNNGG	1 cut(s)	85
Bse1I	ACTGG	6 cut(s)	46, 641, 1251, 1403, 1644, 1783
BseAI	TCCGGA	1 cut(s)	1365
BseBI	CCWGG	2 cut(s)	1182, 1790
BseCI	ATCGAT	1 cut(s)	1104
BseDI	CCNNGG	4 cut(s)	543, 1134, 1221, 1789
BseGI	GGATG	8 cut(s)	391, 443, 517, 622, 742, 1130, 1190, 1416
BseLI	CCNNNNNNNGG	1 cut(s)	85
BseMII	CTCAG	2 cut(s)	140, 1524
BseNI	ACTGG	6 cut(s)	46, 641, 1251, 1403, 1644, 1783
BseRI	GAGGAG	1 cut(s)	410
BseXI	GCAGC	3 cut(s)	452, 643, 1048
BseYI	CCCAGC	1 cut(s)	1673
BsgI	GTGCAG	1 cut(s)	1227
Bsh1236I	CGCG	1 cut(s)	1288
BshNI	GGYRCC	2 cut(s)	636, 922
BshVI	ATCGAT	1 cut(s)	1104
BsiSI	CCGG	3 cut(s)	57, 383, 1366
BslI	CCNNNNNNNGG	1 cut(s)	85
BsmI	GAATGC	1 cut(s)	1603
Bsp119I	TTCGAA	1 cut(s)	1302
Bsp13I	TCCGGA	1 cut(s)	1365
Bsp143I	GATC	4 cut(s)	331, 1036, 1280, 1501
BspACI	CCGC	1 cut(s)	1388
BspCNI	CTCAG	2 cut(s)	139, 1523
BspDI	ATCGAT	1 cut(s)	1104
BspEI	TCCGGA	1 cut(s)	1365
BspFNI	CGCG	1 cut(s)	1288
BspLI	GGNNCC	3 cut(s)	537, 638, 924
BspPI	GGATC	1 cut(s)	1288
BspT104I	TTCGAA	1 cut(s)	1302
BspT107I	GGYRCC	2 cut(s)	636, 922
BsrI	ACTGG	6 cut(s)	46, 641, 1251, 1403, 1644, 1783
BssECI	CCNNGG	4 cut(s)	543, 1134, 1221, 1789
BssMI	GATC	4 cut(s)	331, 1036, 1280, 1501
BssNAI	GTATAC	2 cut(s)	1218, 1255
BssNI	GRCGYC	1 cut(s)	69
BssSI	CACGAG	1 cut(s)	288
BssT1I	CCWWGG	1 cut(s)	1221
Bst1107I	GTATAC	2 cut(s)	1218, 1255
Bst2BI	CACGAG	1 cut(s)	288
Bst2UI	CCWGG	2 cut(s)	1182, 1790
Bst4CI	ACNGT	5 cut(s)	105, 1090, 1346, 1558, 1802
BstACI	GRCGYC	1 cut(s)	69
BstBAI	YACGTR	4 cut(s)	234, 349, 762, 1761
BstBI	TTCGAA	1 cut(s)	1302
BstC8I	GCNNGC	3 cut(s)	27, 987, 991
BstDEI	CTNAG	4 cut(s)	30, 126, 1472, 1510
BstDSI	CCRYGG	1 cut(s)	543
BstF5I	GGATG	8 cut(s)	391, 443, 517, 622, 742, 1130, 1190, 1416
BstFNI	CGCG	1 cut(s)	1288
BstHHI	GCGC	2 cut(s)	98, 1288
BstKTI	GATC	4 cut(s)	334, 1039, 1283, 1504
BstMBI	GATC	4 cut(s)	331, 1036, 1280, 1501
BstMWI	GCNNNNNNNGC	4 cut(s)	66, 379, 464, 826
BstNI	CCWGG	2 cut(s)	1182, 1790
BstNSI	RCATGY	1 cut(s)	989
BstPAI	GACNNNNGTC	1 cut(s)	1737
BstSCI	CCNGG	2 cut(s)	1180, 1788
BstSFI	CTRYAG	2 cut(s)	448, 1497
BstSNI	TACGTA	3 cut(s)	234, 762, 1761
BstUI	CGCG	1 cut(s)	1288
BstV1I	GCAGC	3 cut(s)	452, 643, 1048
BstV2I	GAAGAC	4 cut(s)	20, 853, 1278, 1623
BstX2I	RGATCY	2 cut(s)	1280, 1501
BstYI	RGATCY	2 cut(s)	1280, 1501
BstZ17I	GTATAC	2 cut(s)	1218, 1255
Bsu15I	ATCGAT	1 cut(s)	1104
BsuTUI	ATCGAT	1 cut(s)	1104
BtgI	CCRYGG	1 cut(s)	543
BtsCI	GGATG	8 cut(s)	391, 443, 517, 622, 742, 1130, 1190, 1416
BtsI	GCAGTG	2 cut(s)	565, 1000
BtsIMutI	CAGTG	4 cut(s)	565, 1000, 1244, 1651
Cac8I	GCNNGC	3 cut(s)	27, 987, 991
CfoI	GCGC	2 cut(s)	98, 1288
ClaI	ATCGAT	1 cut(s)	1104
CseI	GACGC	3 cut(s)	58, 398, 446
Csp6I	GTAC	7 cut(s)	49, 400, 637, 923, 1128, 1535, 1745
CviQI	GTAC	7 cut(s)	49, 400, 637, 923, 1128, 1535, 1745
DdeI	CTNAG	4 cut(s)	30, 126, 1472, 1510
DpnI	GATC	4 cut(s)	333, 1038, 1282, 1503
DpnII	GATC	4 cut(s)	331, 1036, 1280, 1501
DraI	TTTAAA	1 cut(s)	1374
DraIII	CACNNNGTG	2 cut(s)	88, 995
Eco105I	TACGTA	3 cut(s)	234, 762, 1761
Eco130I	CCWWGG	1 cut(s)	1221
Eco32I	GATATC	1 cut(s)	1356
Eco57I	CTGAAG	1 cut(s)	1505
Eco72I	CACGTG	1 cut(s)	349
EcoRII	CCWGG	2 cut(s)	1180, 1788
EcoRV	GATATC	1 cut(s)	1356
EcoT14I	CCWWGG	1 cut(s)	1221
ErhI	CCWWGG	1 cut(s)	1221
FalI	AAGNNNNNCTT	4 cut(s)	868, 900, 1377, 1409
FblI	GTMKAC	2 cut(s)	1217, 1254
Fnu4HI	GCNGC	4 cut(s)	441, 657, 1062, 1389
FokI	GGATG	8 cut(s)	398, 430, 524, 629, 749, 1117, 1197, 1423
Fsp4HI	GCNGC	4 cut(s)	441, 657, 1062, 1389
FspBI	CTAG	2 cut(s)	119, 1592
GlaI	GCGC	2 cut(s)	97, 1287
GluI	GCNGC	4 cut(s)	441, 657, 1062, 1389
GsaI	CCCAGC	1 cut(s)	1677
GsuI	CTGGAG	1 cut(s)	227
HapII	CCGG	3 cut(s)	57, 383, 1366
HgaI	GACGC	3 cut(s)	58, 398, 446
HhaI	GCGC	2 cut(s)	98, 1288
Hin1I	GRCGYC	1 cut(s)	69
Hin6I	GCGC	2 cut(s)	96, 1286
HinP1I	GCGC	2 cut(s)	96, 1286
HindIII	AAGCTT	3 cut(s)	465, 664, 882
HinfI	GANTC	4 cut(s)	16, 122, 171, 1639
HpaII	CCGG	3 cut(s)	57, 383, 1366
HphI	GGTGA	2 cut(s)	341, 1241
Hpy166II	GTNNAC	5 cut(s)	91, 1218, 1255, 1292, 1342
Hpy188I	TCNGA	5 cut(s)	12, 1135, 1513, 1524, 1563
Hpy188III	TCNNGA	5 cut(s)	206, 407, 854, 1366, 1394
Hpy8I	GTNNAC	5 cut(s)	91, 1218, 1255, 1292, 1342
HpyAV	CCTTC	6 cut(s)	549, 667, 1319, 1415, 1519, 1583
HpyCH4III	ACNGT	5 cut(s)	105, 1090, 1346, 1558, 1802
HpyCH4IV	ACGT	7 cut(s)	51, 233, 348, 551, 761, 1380, 1760
HpyF10VI	GCNNNNNNNGC	4 cut(s)	66, 379, 464, 826
HpyF3I	CTNAG	4 cut(s)	30, 126, 1472, 1510
HpySE526I	ACGT	7 cut(s)	51, 233, 348, 551, 761, 1380, 1760
Hsp92I	GRCGYC	1 cut(s)	69
HspAI	GCGC	2 cut(s)	96, 1286
Kpn2I	TCCGGA	1 cut(s)	1365
KpnI	GGTACC	2 cut(s)	640, 926
Kzo9I	GATC	4 cut(s)	331, 1036, 1280, 1501
LmnI	GCTCC	2 cut(s)	208, 982
Lsp1109I	GCAGC	3 cut(s)	452, 643, 1048
LweI	GCATC	5 cut(s)	345, 452, 999, 1506, 1716
MaeI	CTAG	2 cut(s)	119, 1592
MaeII	ACGT	7 cut(s)	51, 233, 348, 551, 761, 1380, 1760
MaeIII	GTNAC	9 cut(s)	229, 337, 547, 757, 1193, 1229, 1485, 1577, 1774
MalI	GATC	4 cut(s)	333, 1038, 1282, 1503
MboI	GATC	4 cut(s)	331, 1036, 1280, 1501
MboII	GAAGA	5 cut(s)	25, 853, 1283, 1291, 1628
MflI	RGATCY	2 cut(s)	1280, 1501
MlyI	GAGTC	2 cut(s)	10, 1633
MmeI	TCCRAC	2 cut(s)	489, 1789
MroI	TCCGGA	1 cut(s)	1365
MroXI	GAANNNNTTC	2 cut(s)	1308, 1425
MseI	TTAA	9 cut(s)	42, 251, 612, 645, 1014, 1151, 1236, 1373, 1457
MslI	CAYNNNNRTG	2 cut(s)	24, 830
MspI	CCGG	3 cut(s)	57, 383, 1366
MspR9I	CCNGG	2 cut(s)	1182, 1790
Mva1269I	GAATGC	1 cut(s)	1603
MvaI	CCWGG	2 cut(s)	1182, 1790
MvnI	CGCG	1 cut(s)	1288
MwoI	GCNNNNNNNGC	4 cut(s)	66, 379, 464, 826
NdeII	GATC	4 cut(s)	331, 1036, 1280, 1501
NlaIV	GGNNCC	3 cut(s)	537, 638, 924
NmuCI	GTSAC	3 cut(s)	337, 547, 1229
NspI	RCATGY	1 cut(s)	989
NspV	TTCGAA	1 cut(s)	1302
OliI	CACNNNNGTG	1 cut(s)	24
PaeI	GCATGC	1 cut(s)	989
PctI	GAATGC	1 cut(s)	1603
PdmI	GAANNNNTTC	2 cut(s)	1308, 1425
PfeI	GAWTC	2 cut(s)	122, 171
PflMI	CCANNNNNTGG	1 cut(s)	85
PfoI	TCCNGGA	1 cut(s)	1180
PkrI	GCNGC	4 cut(s)	442, 658, 1063, 1390
PleI	GAGTC	2 cut(s)	10, 1633
PmaCI	CACGTG	1 cut(s)	349
PmlI	CACGTG	1 cut(s)	349
PpsI	GAGTC	2 cut(s)	10, 1633
Ppu21I	YACGTR	4 cut(s)	234, 349, 762, 1761
PshAI	GACNNNNGTC	1 cut(s)	1737
PshBI	ATTAAT	2 cut(s)	1236, 1457
PsiI	TTATAA	2 cut(s)	1208, 1659
Psp6I	CCWGG	2 cut(s)	1180, 1788
PspCI	CACGTG	1 cut(s)	349
PspFI	CCCAGC	1 cut(s)	1673
PspGI	CCWGG	2 cut(s)	1180, 1788
PspN4I	GGNNCC	3 cut(s)	537, 638, 924
PsuI	RGATCY	2 cut(s)	1280, 1501
RsaI	GTAC	7 cut(s)	50, 401, 638, 924, 1129, 1536, 1746
RsaNI	GTAC	7 cut(s)	49, 400, 637, 923, 1128, 1535, 1745
RseI	CAYNNNNRTG	2 cut(s)	24, 830
SaqAI	TTAA	9 cut(s)	42, 251, 612, 645, 1014, 1151, 1236, 1373, 1457
SatI	GCNGC	4 cut(s)	441, 657, 1062, 1389
Sau3AI	GATC	4 cut(s)	331, 1036, 1280, 1501
ScaI	AGTACT	1 cut(s)	401
SchI	GAGTC	2 cut(s)	10, 1633
ScrFI	CCNGG	2 cut(s)	1182, 1790
SfaNI	GCATC	5 cut(s)	345, 452, 999, 1506, 1716
SfcI	CTRYAG	2 cut(s)	448, 1497
SfuI	TTCGAA	1 cut(s)	1302
SmiMI	CAYNNNNRTG	2 cut(s)	24, 830
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
SnaBI	TACGTA	3 cut(s)	234, 762, 1761
SphI	GCATGC	1 cut(s)	989
SsiI	CCGC	1 cut(s)	1388
SspI	AATATT	1 cut(s)	244
SspMI	CTAG	2 cut(s)	119, 1592
StyD4I	CCNGG	2 cut(s)	1180, 1788
StyI	CCWWGG	1 cut(s)	1221
TaaI	ACNGT	5 cut(s)	105, 1090, 1346, 1558, 1802
TaiI	ACGT	7 cut(s)	54, 236, 351, 554, 764, 1383, 1763
TaqI	TCGA	5 cut(s)	169, 174, 406, 1104, 1302
TatI	WGTACW	1 cut(s)	399
TauI	GCSGC	1 cut(s)	1391
TfiI	GAWTC	2 cut(s)	122, 171
Tru1I	TTAA	9 cut(s)	42, 251, 612, 645, 1014, 1151, 1236, 1373, 1457
Tru9I	TTAA	9 cut(s)	42, 251, 612, 645, 1014, 1151, 1236, 1373, 1457
TscAI	CASTG	4 cut(s)	565, 1000, 1251, 1651
TseFI	GTSAC	3 cut(s)	337, 547, 1229
TseI	GCWGC	3 cut(s)	440, 656, 1061
Tsp45I	GTSAC	3 cut(s)	337, 547, 1229
TspDTI	ATGAA	8 cut(s)	528, 686, 709, 848, 853, 1637, 1640, 1806
TspGWI	ACGGA	1 cut(s)	532
TspRI	CASTG	4 cut(s)	565, 1000, 1251, 1651
Van91I	CCANNNNNTGG	1 cut(s)	85
VspI	ATTAAT	2 cut(s)	1236, 1457
XapI	RAATTY	1 cut(s)	694
XceI	RCATGY	1 cut(s)	989
XcmI	CCANNNNNNNNNTGG	1 cut(s)	490
XmiI	GTMKAC	2 cut(s)	1217, 1254
XmnI	GAANNNNTTC	2 cut(s)	1308, 1425
XspI	CTAG	2 cut(s)	119, 1592
ZrmI	AGTACT	1 cut(s)	401

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.