Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0002613

Physical Location & Seq

Genomic Coordinates Reverse (-)

23976932 .. 23979112

Gene Structure 2181 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1151000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2181 bp

ATGGGCTCTCCGAATACTCACGAGCGTGCTAAGATGTTGCTTAACCAGTACGTTTCCGGTGATTCGGGCCCGGTTCCAGGAGTATTTGTCGATCACTTGGTGGACTGCGCCAAAAGGTTTGATTTTGAACTAGAATCTCAGGTTTTTGGCTATTTATTGAACAGTTATGTTAGAGCCAATCGAATCGAATGTGCGATTGATTGCTTTAATAGAATGCTAGAGCATGAGATGTATCCTTGTGTTACGTATGTGAATATTCTTTTAACAGAATTGGTTAGGAGAAATATGATTGGGGAAGCACGAGAGGTGTGTGATAAAATGGCTTTGAGAGGATTTGGAGGTGATCGTGTCACTCTACACGTGATGATGCGTGGTTGTTTGAAGCAGGGGAAGCCGGATGAGGCAGAGGAGTACTTTAGAGAGGCGAGGGCAAGAGGGGTAGAACTTGATGCAGCATCCTATAGTGTTGCCATTGAGGCTTTTTGTAAGAAGCCCAATTCGTGTTTGGCTCTGGAGTTGTTGAATGAAATGAGAAAAATGGGTTGGGTTCCTTCCATGGTCACATTTAGTAGTGTCATCGCAGCTTGTGTAAAGCAGAGGAATATGGTTGAAGCCACGAGGATTAAGGATGGAATGGTAAGTTGTGGGAACCCAATTAATTTGGTTGTTGCTACAAGCTTGATGAAGGGTTATTGTGTGCAAGGGAATTTGGAGAGTGCTTTGGCTTTGTTCAATACGATTATAGAGGATGGACTTACTCCAAACAGGGTTACATATGCAATTCTTATAGAATACTGCTGTAAGAATGGGAACATGGAAAAGGCGTATGAGCTTTACAAGCAAATGAAACATATGAATATTTTGCCTGATGTCTTCATTGTAAATTATTTGATACGAGGATTTTTGAAATATCGGTCATTTGAAGATGCTTCTAAGTTTTTTGATGAGGCCGTTGAGTGTGGTGTTGCTAATGTTTTCTCGTATAATAATATCTTATCATGGCTTTGTGGGAAGGGTAAAGTAAGTGAAGCTTGTAGTTTATGGGATAAAATGATGTCTAAAGGTGTGGTACCTAATGTTGTATCCTACAATAGCAAGATACATGGGTATTGCAGAATAGGGAATATGGAGCATGCGCATGATGTATTCGTGGAGATGCTTAAAAGGGATTTGAAACCAAATGATGTGACTTATTCTATCTTGATAAATGGCTACTTCAAGAACAGTGATGTAGAGCGTGCCTTTGATGTCTTTGATGATATGGTGGCTGCCAAGATTTCTCCCACAGGCTTCACAGTTGGCATTGTCATTGATGGCTTGTGCAGAGCTGGTCGTACGTCTGAGGCAAGTGAGATGCTAAAGAAAATTGTTGCAAGGGGTTTTGTTCCTGGATGTATTAGTTACAATAGCATTATCGATGGGTATATCAAGGAGGGTGACATTAATTCTGCACTGGCTGTGTACAGAGAAATGTGTGAAGGTGAAGTCTCCCCCAATGTTGTCACTTACACCAGCATTATTAATGGGTTTTGCAAAAGCAATCAAATTGATATGGCTTTGAAAATGTGGAATGAGATGAAAAACAAGGGCATCGAAGTGGATGTTACTGCATATTCTGCTCTCATTGATGGGTTTTGCAAAAGACGAGACATGAGAACTGCCCATGAACTTTTCTCAGAACTCCTTGAAGTTGGTTTATCTCCAAATGTAGTTGTTTACAGTAGCATGATATCTGGCTTCCGGAATTTAAATAACATGGAAGCAGCCCTTGACATGCACAACAAGATGATTAGAGACGGTATTCCTTGTGATTTGAAGGCATACACTACGTTAATCGATGGATTGCTAAGAGAAGGTGAGTTACAATCTGCTACAGATCTCTACTCAGAGATGCTTCAGAAGGGAATTATACCTGATACAAACACATATACGGTTCTGATAAATGCCCTTCATAACAAAGGACAGCTAGAGAGTGCACGCAAGAACTTGGAAGACATGGATAGGCGGGGTATGACTCCTAGTGTTCATTTTTATAATACTCTGATTGCTGGAAACTTCAAGGAGGGTAATTTGCAAGAGGCTTTTAGGTTGCATGATGAAATGCTTGACAAAGGCCTTGTTCCAGATGACAATACATATGATACTCTTGTAAATGGGAAGTTCCAAGGTACAAGGATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

727

Amino Acids

81.54

Weight (kDa)

5.7

Isoelectric Point (pI)

32.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	116 - 163	3.5e-08	PPR repeat family
TPR_24	PF23276	126 - 204	7.7e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	139 - 198	9.2e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	158 - 198	3.1e-06	PPR repeat family
PPR_3	PF13812	207 - 269	1e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	220 - 264	1.6e-09	PPR repeat family
PPR_1	PF12854	250 - 283	3.2e-10	PPR repeat
PPR_2	PF13041	254 - 303	6.5e-13	PPR repeat family
PPR	PF01535	257 - 287	1e-06	PPR repeat
PPR_long	PF17177	313 - 456	3e-08	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	324 - 372	1.2e-15	PPR repeat family
PPR_3	PF13812	347 - 405	2.7e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	354 - 386	3.5e-09	PPR repeat
PPR_2	PF13041	358 - 407	9.7e-19	PPR repeat family
PPR	PF01535	361 - 390	7.5e-06	PPR repeat
PPR_1	PF12854	390 - 422	4e-11	PPR repeat
PPR	PF01535	396 - 423	6e-07	PPR repeat
PPR_2	PF13041	396 - 442	1.4e-13	PPR repeat family
PPR_1	PF12854	426 - 455	5.2e-06	PPR repeat
TPR_24	PF23276	441 - 560	2.1e-12	Fungal tetratrico peptide repeats
PPR_3	PF13812	453 - 510	1.9e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	459 - 491	2.4e-06	PPR repeat
PPR_2	PF13041	465 - 512	4.3e-17	PPR repeat family
PPR	PF01535	466 - 493	4.8e-06	PPR repeat
PPR_long	PF17177	476 - 613	2.3e-12	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	496 - 527	4.3e-12	PPR repeat
PPR_2	PF13041	498 - 547	5.3e-20	PPR repeat family
PPR	PF01535	501 - 531	1.9e-09	PPR repeat
PPR_3	PF13812	523 - 579	5.8e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	529 - 560	1.9e-06	PPR repeat
PPR_2	PF13041	534 - 580	4.4e-12	PPR repeat family
PPR_2	PF13041	568 - 616	2.9e-11	PPR repeat family
PPR_3	PF13812	594 - 650	7.4e-07	Pentatricopeptide repeat domain
TPR_24	PF23276	596 - 700	5.4e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	607 - 651	2.2e-14	PPR repeat family
PPR_3	PF13812	663 - 717	1.8e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	669 - 701	5.4e-07	PPR repeat
PPR_2	PF13041	673 - 719	4.4e-13	PPR repeat family
PPR	PF01535	678 - 706	4.3e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39230 AT3G54980 AT3G54980
fragaria_vesca	FvH4_5g25890 FvH4_5g25890 FvH4_5g25890
malus_domestica	MD04G1151000.v1.1 MD12G1164900.v1.1
prunus_persica	Prupe.6G275900_v2.0.a1 Prupe.6G275900_v2.0.a1
pyrus_communis	pycom04g13670 pycom12g15770
rosa_chinensis	RchiOBHm_Chr4g0410561 RchiOBHm_Chr4g0410571 RchiOBHm_Chr5g0071501 RchiOBHm_Chr6g0264781 RchiOBHm_Chr7g0216391 RchiOBHm_Chr7g0216401
rosa_laevigata	RLG00000002575 RLG00000002576 RLG00000002577 RLG00000031675
rosa_multiflora	Rmu_sc0000692.1_g000003 Rmu_sc0000692.1_g000005 Rmu_sc0006325.1_g000046
rosa_roxburghii	Rroxscaffold_159G00432870 Rroxscaffold_3G00243290 Rroxscaffold_4G00298080
rosa_rugosa	Rorug01G0081600 Rorug07G0160300
rosa_samantha	Rh2DG118300 Rh4CG174000 Rh4CG174100 Rh4CG174200 Rh7AG298700 Rh7AG298800 Rh7BG290300 Rh7BG290400
rosa_wichuraiana	Rw7G025370 Rw7G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2034
Acc16I	TGCGCA	1 cut(s)	1137
Acc65I	GGTACC	1 cut(s)	1069
AccB1I	GGYRCC	1 cut(s)	1069
AccIII	TCCGGA	1 cut(s)	1740
AciI	CCGC	1 cut(s)	2005
AcsI	RAATTY	2 cut(s)	706, 1744
AcuI	CTGAAG	1 cut(s)	1880
AcvI	CACGTG	1 cut(s)	361
AdeI	CACNNNGTG	1 cut(s)	100
AfaI	GTAC	6 cut(s)	50, 413, 1071, 1336, 1463, 2170
AfiI	CCNNNNNNNGG	1 cut(s)	77
AflIII	ACRYGT	1 cut(s)	358
AjnI	CCWGG	2 cut(s)	76, 1387
AjuI	GAANNNNNNNTTGG	2 cut(s)	526, 558
AleI	CACNNNNGTG	1 cut(s)	24
AluBI	AGCT	6 cut(s)	584, 678, 832, 1031, 1328, 1966
AluI	AGCT	6 cut(s)	584, 678, 832, 1031, 1328, 1966
Alw21I	GWGCWC	1 cut(s)	1978
Alw26I	GTCTC	3 cut(s)	1492, 1641, 1788
Alw44I	GTGCAC	1 cut(s)	1974
Aor13HI	TCCGGA	1 cut(s)	1740
AoxI	GGCC	3 cut(s)	67, 948, 2113
ApaI	GGGCCC	1 cut(s)	71
ApaLI	GTGCAC	1 cut(s)	1974
ApeKI	GCWGC	4 cut(s)	452, 581, 1268, 1763
ApoI	RAATTY	2 cut(s)	706, 1744
AseI	ATTAAT	3 cut(s)	657, 1443, 1521
Asp718I	GGTACC	1 cut(s)	1069
AspLEI	GCGC	2 cut(s)	110, 1138
AspS9I	GGNCC	2 cut(s)	67, 68
AsuC2I	CCSGG	1 cut(s)	71
AsuHPI	GGTGA	5 cut(s)	71, 353, 1448, 1493, 1868
BaeGI	GKGCMC	2 cut(s)	71, 1978
BaeI	ACNNNNGTAYC	4 cut(s)	1091, 1124, 2133, 2166
BanI	GGYRCC	1 cut(s)	1069
BanII	GRGCYC	2 cut(s)	8, 71
BarI	GAAGNNNNNNTAC	2 cut(s)	1845, 1877
BauI	CACGAG	3 cut(s)	20, 300, 616
BbrPI	CACGTG	1 cut(s)	361
BbsI	GAAGAC	2 cut(s)	865, 1998
Bbv12I	GWGCWC	1 cut(s)	1978
BbvI	GCAGC	4 cut(s)	464, 593, 1255, 1775
BccI	CCATC	6 cut(s)	623, 743, 1307, 1412, 1622, 1832
BceAI	ACGGC	1 cut(s)	935
BciT130I	CCWGG	2 cut(s)	78, 1389
BciVI	GTATCC	2 cut(s)	243, 1093
BcnI	CCSGG	1 cut(s)	71
BcoDI	GTCTC	3 cut(s)	1492, 1641, 1788
BfaI	CTAG	4 cut(s)	131, 218, 1967, 2019
BfmI	CTRYAG	2 cut(s)	460, 1872
BfuI	GTATCC	2 cut(s)	243, 1093
BglI	GCCNNNNNGGC	1 cut(s)	476
BglII	AGATCT	1 cut(s)	1876
BisI	GCNGC	4 cut(s)	453, 582, 1269, 1764
BlsI	GCNGC	4 cut(s)	454, 583, 1270, 1765
BmcAI	AGTACT	1 cut(s)	413
Bme1390I	CCNGG	3 cut(s)	71, 78, 1389
BmgT120I	GGNCC	2 cut(s)	67, 68
BmiI	GGNNCC	5 cut(s)	69, 75, 549, 650, 1071
BmrFI	CCNGG	3 cut(s)	71, 78, 1389
BmsI	GCATC	8 cut(s)	357, 439, 464, 916, 1146, 1344, 1599, 1881
BpiI	GAAGAC	2 cut(s)	865, 1998
BpmI	CTGGAG	1 cut(s)	533
BpuMI	CCSGG	1 cut(s)	71
Bsa29I	ATCGAT	2 cut(s)	1416, 1836
BsaAI	YACGTR	2 cut(s)	246, 361
BsaJI	CCNNGG	2 cut(s)	555, 2164
BsaWI	WCCGGW	2 cut(s)	56, 1740
BsaXI	ACNNNNNCTCC	2 cut(s)	72, 102
Bsc4I	CCNNNNNNNGG	1 cut(s)	77
Bse1I	ACTGG	2 cut(s)	46, 1458
BseAI	TCCGGA	1 cut(s)	1740
BseBI	CCWGG	2 cut(s)	78, 1389
BseCI	ATCGAT	2 cut(s)	1416, 1836
BseDI	CCNNGG	2 cut(s)	555, 2164
BseGI	GGATG	6 cut(s)	403, 455, 634, 754, 1397, 1606
BseLI	CCNNNNNNNGG	1 cut(s)	77
BseMII	CTCAG	4 cut(s)	152, 1332, 1689, 1899
BseNI	ACTGG	2 cut(s)	46, 1458
BseRI	GAGGAG	1 cut(s)	422
BseSI	GKGCMC	2 cut(s)	71, 1978
BseXI	GCAGC	4 cut(s)	464, 593, 1255, 1775
BsgI	GTGCAG	2 cut(s)	1342, 1434
BshFI	GGCC	3 cut(s)	69, 950, 2115
BshNI	GGYRCC	1 cut(s)	1069
BshVI	ATCGAT	2 cut(s)	1416, 1836
BsiHKAI	GWGCWC	1 cut(s)	1978
BsiSI	CCGG	4 cut(s)	57, 71, 395, 1741
BsiWI	CGTACG	1 cut(s)	1334
BslI	CCNNNNNNNGG	1 cut(s)	77
BsmAI	GTCTC	3 cut(s)	1492, 1641, 1788
BsmBI	CGTCTC	1 cut(s)	1788
BsmI	GAATGC	1 cut(s)	219
BsnI	GGCC	3 cut(s)	69, 950, 2115
Bsp120I	GGGCCC	1 cut(s)	67
Bsp1286I	GDGCHC	3 cut(s)	8, 71, 1978
Bsp13I	TCCGGA	1 cut(s)	1740
Bsp1407I	TGTACA	1 cut(s)	1461
Bsp143I	GATC	3 cut(s)	91, 343, 1876
Bsp19I	CCATGG	1 cut(s)	555
BspACI	CCGC	1 cut(s)	2005
BspANI	GGCC	3 cut(s)	69, 950, 2115
BspCNI	CTCAG	4 cut(s)	151, 1333, 1688, 1898
BspDI	ATCGAT	2 cut(s)	1416, 1836
BspEI	TCCGGA	1 cut(s)	1740
BspLI	GGNNCC	5 cut(s)	69, 75, 549, 650, 1071
BspT107I	GGYRCC	1 cut(s)	1069
BsrGI	TGTACA	1 cut(s)	1461
BsrI	ACTGG	2 cut(s)	46, 1458
BssECI	CCNNGG	2 cut(s)	555, 2164
BssMI	GATC	3 cut(s)	91, 343, 1876
BssSI	CACGAG	3 cut(s)	20, 300, 616
BssT1I	CCWWGG	2 cut(s)	555, 2164
Bst2BI	CACGAG	3 cut(s)	20, 300, 616
Bst2UI	CCWGG	2 cut(s)	78, 1389
Bst4CI	ACNGT	6 cut(s)	164, 1226, 1297, 1721, 1799, 1933
BstAPI	GCANNNNNTGC	1 cut(s)	1616
BstAUI	TGTACA	1 cut(s)	1461
BstBAI	YACGTR	2 cut(s)	246, 361
BstC8I	GCNNGC	4 cut(s)	27, 1134, 1239, 1978
BstDEI	CTNAG	7 cut(s)	30, 138, 933, 1341, 1675, 1847, 1885
BstDSI	CCRYGG	1 cut(s)	555
BstF5I	GGATG	6 cut(s)	403, 455, 634, 754, 1397, 1606
BstHHI	GCGC	2 cut(s)	110, 1138
BstKTI	GATC	3 cut(s)	94, 346, 1879
BstMAI	GTCTC	3 cut(s)	1492, 1641, 1788
BstMBI	GATC	3 cut(s)	91, 343, 1876
BstMWI	GCNNNNNNNGC	4 cut(s)	391, 476, 838, 1616
BstNI	CCWGG	2 cut(s)	78, 1389
BstNSI	RCATGY	2 cut(s)	1136, 1777
BstSCI	CCNGG	3 cut(s)	69, 76, 1387
BstSFI	CTRYAG	2 cut(s)	460, 1872
BstSLI	GKGCMC	2 cut(s)	71, 1978
BstSNI	TACGTA	1 cut(s)	246
BstV1I	GCAGC	4 cut(s)	464, 593, 1255, 1775
BstV2I	GAAGAC	2 cut(s)	865, 1998
BstX2I	RGATCY	1 cut(s)	1876
BstYI	RGATCY	1 cut(s)	1876
Bsu15I	ATCGAT	2 cut(s)	1416, 1836
BsuI	GTATCC	2 cut(s)	243, 1093
BsuRI	GGCC	3 cut(s)	69, 950, 2115
BsuTUI	ATCGAT	2 cut(s)	1416, 1836
BtgI	CCRYGG	1 cut(s)	555
BtgZI	GCGATG	1 cut(s)	562
BtsCI	GGATG	6 cut(s)	403, 455, 634, 754, 1397, 1606
BtsIMutI	CAGTG	2 cut(s)	1231, 1451
Cac8I	GCNNGC	4 cut(s)	27, 1134, 1239, 1978
CfoI	GCGC	2 cut(s)	110, 1138
Cfr13I	GGNCC	2 cut(s)	67, 68
ClaI	ATCGAT	2 cut(s)	1416, 1836
Csp6I	GTAC	6 cut(s)	49, 412, 1070, 1335, 1462, 2169
CviQI	GTAC	6 cut(s)	49, 412, 1070, 1335, 1462, 2169
DdeI	CTNAG	7 cut(s)	30, 138, 933, 1341, 1675, 1847, 1885
DpnI	GATC	3 cut(s)	93, 345, 1878
DpnII	GATC	3 cut(s)	91, 343, 1876
DraI	TTTAAA	1 cut(s)	1749
DraIII	CACNNNGTG	1 cut(s)	100
Eco105I	TACGTA	1 cut(s)	246
Eco130I	CCWWGG	2 cut(s)	555, 2164
Eco147I	AGGCCT	1 cut(s)	2115
Eco24I	GRGCYC	2 cut(s)	8, 71
Eco32I	GATATC	1 cut(s)	1731
Eco57I	CTGAAG	1 cut(s)	1880
Eco72I	CACGTG	1 cut(s)	361
EcoRII	CCWGG	2 cut(s)	76, 1387
EcoRV	GATATC	1 cut(s)	1731
EcoT14I	CCWWGG	2 cut(s)	555, 2164
EcoT38I	GRGCYC	2 cut(s)	8, 71
ErhI	CCWWGG	2 cut(s)	555, 2164
Esp3I	CGTCTC	1 cut(s)	1788
FalI	AAGNNNNNCTT	4 cut(s)	1015, 1047, 1752, 1784
FauI	CCCGC	1 cut(s)	1998
FauNDI	CATATG	3 cut(s)	775, 852, 2137
Fnu4HI	GCNGC	4 cut(s)	453, 582, 1269, 1764
FokI	GGATG	6 cut(s)	410, 442, 641, 761, 1404, 1613
FriOI	GRGCYC	2 cut(s)	8, 71
Fsp4HI	GCNGC	4 cut(s)	453, 582, 1269, 1764
FspAI	RTGCGCAY	1 cut(s)	1137
FspBI	CTAG	4 cut(s)	131, 218, 1967, 2019
FspI	TGCGCA	1 cut(s)	1137
GlaI	GCGC	2 cut(s)	109, 1137
GluI	GCNGC	4 cut(s)	453, 582, 1269, 1764
GsuI	CTGGAG	1 cut(s)	533
HaeIII	GGCC	3 cut(s)	69, 950, 2115
HapII	CCGG	4 cut(s)	57, 71, 395, 1741
HhaI	GCGC	2 cut(s)	110, 1138
Hin6I	GCGC	2 cut(s)	108, 1136
HinP1I	GCGC	2 cut(s)	108, 1136
HindIII	AAGCTT	2 cut(s)	676, 1029
HinfI	GANTC	4 cut(s)	62, 134, 183, 2014
HpaII	CCGG	4 cut(s)	57, 71, 395, 1741
HphI	GGTGA	5 cut(s)	71, 353, 1448, 1493, 1868
Hpy166II	GTNNAC	4 cut(s)	103, 1462, 1717, 1976
Hpy188I	TCNGA	7 cut(s)	12, 1342, 1678, 1888, 1899, 1938, 2043
Hpy188III	TCNNGA	6 cut(s)	20, 512, 1201, 1219, 1741, 2123
Hpy8I	GTNNAC	4 cut(s)	103, 1462, 1717, 1976
HpyAV	CCTTC	8 cut(s)	561, 679, 1006, 1472, 1810, 1847, 1894, 1958
HpyCH4III	ACNGT	6 cut(s)	164, 1226, 1297, 1721, 1799, 1933
HpyCH4IV	ACGT	5 cut(s)	51, 245, 360, 1337, 1829
HpyF10VI	GCNNNNNNNGC	4 cut(s)	391, 476, 838, 1616
HpyF3I	CTNAG	7 cut(s)	30, 138, 933, 1341, 1675, 1847, 1885
HpySE526I	ACGT	5 cut(s)	51, 245, 360, 1337, 1829
HspAI	GCGC	2 cut(s)	108, 1136
Kpn2I	TCCGGA	1 cut(s)	1740
KpnI	GGTACC	1 cut(s)	1073
Kzo9I	GATC	3 cut(s)	91, 343, 1876
LmnI	GCTCC	1 cut(s)	1129
Lsp1109I	GCAGC	4 cut(s)	464, 593, 1255, 1775
LweI	GCATC	8 cut(s)	357, 439, 464, 916, 1146, 1344, 1599, 1881
MaeI	CTAG	4 cut(s)	131, 218, 1967, 2019
MaeII	ACGT	5 cut(s)	51, 245, 360, 1337, 1829
MalI	GATC	3 cut(s)	93, 345, 1878
MboI	GATC	3 cut(s)	91, 343, 1876
MboII	GAAGA	3 cut(s)	865, 935, 2003
MflI	RGATCY	1 cut(s)	1876
MhlI	GDGCHC	3 cut(s)	8, 71, 1978
MlyI	GAGTC	1 cut(s)	2008
MroI	TCCGGA	1 cut(s)	1740
MslI	CAYNNNNRTG	3 cut(s)	24, 1137, 1595
MspI	CCGG	4 cut(s)	57, 71, 395, 1741
MspR9I	CCNGG	3 cut(s)	71, 78, 1389
Mva1269I	GAATGC	1 cut(s)	219
MvaI	CCWGG	2 cut(s)	78, 1389
MwoI	GCNNNNNNNGC	4 cut(s)	391, 476, 838, 1616
NciI	CCSGG	1 cut(s)	71
NcoI	CCATGG	1 cut(s)	555
NdeI	CATATG	3 cut(s)	775, 852, 2137
NdeII	GATC	3 cut(s)	91, 343, 1876
NlaIV	GGNNCC	5 cut(s)	69, 75, 549, 650, 1071
NmuCI	GTSAC	5 cut(s)	349, 559, 1186, 1436, 1501
NsbI	TGCGCA	1 cut(s)	1137
NspI	RCATGY	2 cut(s)	1136, 1777
OliI	CACNNNNGTG	1 cut(s)	24
PaeI	GCATGC	1 cut(s)	1136
PceI	AGGCCT	1 cut(s)	2115
PctI	GAATGC	1 cut(s)	219
PfeI	GAWTC	3 cut(s)	62, 134, 183
Pfl23II	CGTACG	1 cut(s)	1334
PfoI	TCCNGGA	2 cut(s)	76, 1387
PkrI	GCNGC	4 cut(s)	454, 583, 1270, 1765
PleI	GAGTC	1 cut(s)	2008
PmaCI	CACGTG	1 cut(s)	361
PmlI	CACGTG	1 cut(s)	361
PpsI	GAGTC	1 cut(s)	2008
Ppu21I	YACGTR	2 cut(s)	246, 361
PshBI	ATTAAT	3 cut(s)	657, 1443, 1521
PsiI	TTATAA	1 cut(s)	2034
Psp6I	CCWGG	2 cut(s)	76, 1387
PspCI	CACGTG	1 cut(s)	361
PspGI	CCWGG	2 cut(s)	76, 1387
PspLI	CGTACG	1 cut(s)	1334
PspN4I	GGNNCC	5 cut(s)	69, 75, 549, 650, 1071
PspOMI	GGGCCC	1 cut(s)	67
PspPI	GGNCC	2 cut(s)	67, 68
PsuI	RGATCY	1 cut(s)	1876
RsaI	GTAC	6 cut(s)	50, 413, 1071, 1336, 1463, 2170
RsaNI	GTAC	6 cut(s)	49, 412, 1070, 1335, 1462, 2169
RseI	CAYNNNNRTG	3 cut(s)	24, 1137, 1595
SatI	GCNGC	4 cut(s)	453, 582, 1269, 1764
Sau3AI	GATC	3 cut(s)	91, 343, 1876
Sau96I	GGNCC	2 cut(s)	67, 68
ScaI	AGTACT	1 cut(s)	413
SchI	GAGTC	1 cut(s)	2008
ScrFI	CCNGG	3 cut(s)	71, 78, 1389
SduI	GDGCHC	3 cut(s)	8, 71, 1978
SfaNI	GCATC	8 cut(s)	357, 439, 464, 916, 1146, 1344, 1599, 1881
SfcI	CTRYAG	2 cut(s)	460, 1872
SmiI	ATTTAAAT	1 cut(s)	1749
SmiMI	CAYNNNNRTG	3 cut(s)	24, 1137, 1595
SnaBI	TACGTA	1 cut(s)	246
SphI	GCATGC	1 cut(s)	1136
SseBI	AGGCCT	1 cut(s)	2115
SsiI	CCGC	1 cut(s)	2005
SspI	AATATT	2 cut(s)	256, 859
SspMI	CTAG	4 cut(s)	131, 218, 1967, 2019
StuI	AGGCCT	1 cut(s)	2115
StyD4I	CCNGG	3 cut(s)	69, 76, 1387
StyI	CCWWGG	2 cut(s)	555, 2164
SwaI	ATTTAAAT	1 cut(s)	1749
TaaI	ACNGT	6 cut(s)	164, 1226, 1297, 1721, 1799, 1933
TaiI	ACGT	5 cut(s)	54, 248, 363, 1340, 1832
TaqI	TCGA	6 cut(s)	90, 181, 186, 1416, 1593, 1836
TaqII	GACCGA	1 cut(s)	903
TatI	WGTACW	2 cut(s)	411, 1461
TfiI	GAWTC	3 cut(s)	62, 134, 183
TscAI	CASTG	2 cut(s)	1231, 1458
TseFI	GTSAC	5 cut(s)	349, 559, 1186, 1436, 1501
TseI	GCWGC	4 cut(s)	452, 581, 1268, 1763
Tsp45I	GTSAC	5 cut(s)	349, 559, 1186, 1436, 1501
TspRI	CASTG	2 cut(s)	1231, 1458
VneI	GTGCAC	1 cut(s)	1974
VspI	ATTAAT	3 cut(s)	657, 1443, 1521
XapI	RAATTY	2 cut(s)	706, 1744
XceI	RCATGY	2 cut(s)	1136, 1777
XcmI	CCANNNNNNNNNTGG	1 cut(s)	502
XspI	CTAG	4 cut(s)	131, 218, 1967, 2019
ZrmI	AGTACT	1 cut(s)	413

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.