RchiOBHm_Chr4g0410571

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002613
Physical Location & Seq
Forward (+)
34358148 .. 34359093
946 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38147

Sequence Viewer

Length: 675 bp
ATGCAAAACTGCAAAAGCAATAAGATAGATCTTGCTTTAGAAATGTGGCATGAAATGAAAAGTAAGGGCATCAAAATGGATGTTACAGCATACTCTGCTATCATTGATGGGTTTTGCAAAAGGCGAGACATGAAAAGTGCTCGCGAACTTTTCTCGGAACTTCTGGAAGTTGGTTTATCTCCAAATACAGGTGTTTATACTAGCATGATTTGTGGCTTCCGGAATGTAAATGACATGGAAGCAGCTATTGACTTACATAAGAAAATGATAACTGAAGGCATTCCATGTGATGTGAAAACGTACACTGCATTAATCAATGGACTGCTAAGAAAGGGTGATTTACAATCTGCATCAGATCTTTACTCAAAGATGCTTTCGAAGGGAATCACACCTGATATCAACACATATACAGTTCTAATAAATGGCCTTTGTTGCAAACGACAATTGGAGAAGGCACGCAAAACCTTGGAATACATGAATAGAAGGAGAATGACTGCTAGGGTTCATATTTATACTGCTTTGATTGTTGGGCACTTCAAGGAGGGGAATTTGCAAGAATCGTTTAGGTTGCACGATGAAATGCTAGACAAAGGCCTCGTACCTGATGACATTACTTGTGATATTCTTATAAATGGAAAGTTCAAAGGCATAAGTGCTCTTCCGAGGACTTCATGA

Protein Analysis

224

Amino Acids

25.32

Weight (kDa)

8.89

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 4 - 40 8.1e-11 PPR repeat family
PPR_long PF17177 6 - 113 2.5e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 7 - 123 2.5e-10 Fungal tetratrico peptide repeats
PPR_3 PF13812 16 - 70 1.6e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 22 - 53 1.6e-07 PPR repeat
PPR_2 PF13041 27 - 73 4.6e-11 PPR repeat family
PPR_2 PF13041 61 - 109 2.7e-12 PPR repeat family
PPR_3 PF13812 87 - 141 1e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 97 - 144 1.2e-17 PPR repeat family
PPR PF01535 100 - 129 9.6e-07 PPR repeat
PPR_1 PF12854 127 - 159 1.3e-08 PPR repeat
PPR_2 PF13041 168 - 212 5.4e-11 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AccII CGCG 1 cut(s) 144
AccIII TCCGGA 1 cut(s) 219
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 2 cut(s) 302, 600
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 2 cut(s) 538, 643
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw21I GWGCWC 2 cut(s) 142, 658
Alw26I GTCTC 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 219
AoxI GGCC 2 cut(s) 424, 592
ApeKI GCWGC 1 cut(s) 242
ApoI RAATTY 1 cut(s) 547
AseI ATTAAT 1 cut(s) 311
Asp700I GAANNNNTTC 1 cut(s) 279
AsuHPI GGTGA 1 cut(s) 347
AsuII TTCGAA 1 cut(s) 377
BaeGI GKGCMC 1 cut(s) 534
Bbv12I GWGCWC 2 cut(s) 142, 658
BbvI GCAGC 1 cut(s) 254
BccI CCATC 1 cut(s) 101
BcoDI GTCTC 1 cut(s) 120
BfaI CTAG 3 cut(s) 201, 498, 584
BglII AGATCT 2 cut(s) 28, 355
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
BmsI GCATC 3 cut(s) 78, 359, 360
Bpu14I TTCGAA 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 465, 662
BsaWI WCCGGW 1 cut(s) 219
Bsc4I CCNNNNNNNGG 1 cut(s) 188
BseAI TCCGGA 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 465, 662
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 188
BseSI GKGCMC 1 cut(s) 534
BseXI GCAGC 1 cut(s) 254
Bsh1236I CGCG 1 cut(s) 144
BshFI GGCC 2 cut(s) 426, 594
BsiHKAI GWGCWC 2 cut(s) 142, 658
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 120
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 2 cut(s) 426, 594
Bsp119I TTCGAA 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 142, 534, 658
Bsp13I TCCGGA 1 cut(s) 219
Bsp143I GATC 2 cut(s) 28, 355
Bsp68I TCGCGA 1 cut(s) 144
BspANI GGCC 2 cut(s) 426, 594
BspEI TCCGGA 1 cut(s) 219
BspFNI CGCG 1 cut(s) 144
BspHI TCATGA 1 cut(s) 671
BspQI GCTCTTC 1 cut(s) 663
BspT104I TTCGAA 1 cut(s) 377
BssECI CCNNGG 2 cut(s) 465, 662
BssMI GATC 2 cut(s) 28, 355
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 412
Bst6I CTCTTC 1 cut(s) 663
BstAPI GCANNNNNTGC 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 142, 457
BstDEI CTNAG 1 cut(s) 326
BstF5I GGATG 1 cut(s) 85
BstFNI CGCG 1 cut(s) 144
BstKTI GATC 2 cut(s) 31, 358
BstMAI GTCTC 1 cut(s) 120
BstMBI GATC 2 cut(s) 28, 355
BstMWI GCNNNNNNNGC 2 cut(s) 95, 432
BstSLI GKGCMC 1 cut(s) 534
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 1 cut(s) 254
BstX2I RGATCY 2 cut(s) 28, 355
BstYI RGATCY 2 cut(s) 28, 355
BsuRI GGCC 2 cut(s) 426, 594
BtsCI GGATG 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 1 cut(s) 303
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 142, 457
CciI TCATGA 1 cut(s) 671
Csp6I GTAC 2 cut(s) 301, 599
CviAII CATG 7 cut(s) 50, 130, 205, 235, 285, 475, 672
CviJI RGCY 4 cut(s) 216, 245, 426, 594
CviKI_1 RGCY 4 cut(s) 216, 245, 426, 594
CviQI GTAC 2 cut(s) 301, 599
DdeI CTNAG 1 cut(s) 326
DpnI GATC 2 cut(s) 30, 357
DpnII GATC 2 cut(s) 28, 355
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 465
Eco147I AGGCCT 1 cut(s) 594
Eco32I GATATC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 7 cut(s) 53, 133, 208, 238, 288, 478, 675
FatI CATG 7 cut(s) 49, 129, 204, 234, 284, 474, 671
Fnu4HI GCNGC 1 cut(s) 243
FokI GGATG 1 cut(s) 92
Fsp4HI GCNGC 1 cut(s) 243
FspBI CTAG 3 cut(s) 201, 498, 584
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 2 cut(s) 426, 594
HapII CCGG 1 cut(s) 220
Hin1II CATG 7 cut(s) 53, 133, 208, 238, 288, 478, 675
HinfI GANTC 2 cut(s) 384, 557
HpaII CCGG 1 cut(s) 220
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 1 cut(s) 303
Hpy188I TCNGA 3 cut(s) 157, 355, 663
Hpy188III TCNNGA 4 cut(s) 143, 164, 220, 672
Hpy8I GTNNAC 1 cut(s) 303
HpyAV CCTTC 4 cut(s) 269, 373, 445, 477
HpyCH4III ACNGT 1 cut(s) 412
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 8 cut(s) 4, 12, 117, 308, 350, 435, 553, 571
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 432
HpyF3I CTNAG 1 cut(s) 326
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 7 cut(s) 53, 133, 208, 238, 288, 478, 675
Kpn2I TCCGGA 1 cut(s) 219
Kzo9I GATC 2 cut(s) 28, 355
LguI GCTCTTC 1 cut(s) 663
LpnPI CCDG 5 cut(s) 149, 174, 233, 405, 615
Lsp1109I GCAGC 1 cut(s) 254
LweI GCATC 3 cut(s) 78, 359, 360
MaeI CTAG 3 cut(s) 201, 498, 584
MaeII ACGT 1 cut(s) 299
MaeIII GTNAC 1 cut(s) 82
MalI GATC 2 cut(s) 30, 357
MboI GATC 2 cut(s) 28, 355
MboII GAAGA 1 cut(s) 650
MfeI CAATTG 1 cut(s) 443
MflI RGATCY 2 cut(s) 28, 355
MhlI GDGCHC 3 cut(s) 142, 534, 658
MluCI AATT 2 cut(s) 443, 547
MnlI CCTC 3 cut(s) 535, 605, 657
MroI TCCGGA 1 cut(s) 219
MroXI GAANNNNTTC 1 cut(s) 279
MseI TTAA 1 cut(s) 311
MslI CAYNNNNRTG 1 cut(s) 74
MspI CCGG 1 cut(s) 220
MunI CAATTG 1 cut(s) 443
Mva1269I GAATGC 1 cut(s) 279
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 2 cut(s) 95, 432
NdeII GATC 2 cut(s) 28, 355
NlaIII CATG 7 cut(s) 53, 133, 208, 238, 288, 478, 675
NruI TCGCGA 1 cut(s) 144
NspV TTCGAA 1 cut(s) 377
PagI TCATGA 1 cut(s) 671
PceI AGGCCT 1 cut(s) 594
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 279
PdmI GAANNNNTTC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 384, 557
PkrI GCNGC 1 cut(s) 244
PshBI ATTAAT 1 cut(s) 311
PsiI TTATAA 1 cut(s) 629
PsuI RGATCY 2 cut(s) 28, 355
RruI TCGCGA 1 cut(s) 144
RsaI GTAC 2 cut(s) 302, 600
RsaNI GTAC 2 cut(s) 301, 599
RseI CAYNNNNRTG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 1 cut(s) 311
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 2 cut(s) 28, 355
SduI GDGCHC 3 cut(s) 142, 534, 658
SetI ASST 7 cut(s) 193, 247, 302, 394, 467, 569, 604
SfaNI GCATC 3 cut(s) 78, 359, 360
SfuI TTCGAA 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 74
Sse9I AATT 2 cut(s) 443, 547
SseBI AGGCCT 1 cut(s) 594
SspMI CTAG 3 cut(s) 201, 498, 584
StuI AGGCCT 1 cut(s) 594
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 412
TaiI ACGT 1 cut(s) 302
TaqI TCGA 1 cut(s) 377
TasI AATT 2 cut(s) 443, 547
TfiI GAWTC 2 cut(s) 384, 557
Tru1I TTAA 1 cut(s) 311
Tru9I TTAA 1 cut(s) 311
TscAI CASTG 1 cut(s) 310
TseI GCWGC 1 cut(s) 242
TspDTI ATGAA 7 cut(s) 66, 71, 146, 491, 494, 591, 660
TspRI CASTG 1 cut(s) 310
VspI ATTAAT 1 cut(s) 311
XapI RAATTY 1 cut(s) 547
XmnI GAANNNNTTC 1 cut(s) 279
XspI CTAG 3 cut(s) 201, 498, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.