Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0002613

Physical Location & Seq

Genomic Coordinates Forward (+)

35818435 .. 35826209

Gene Structure 7775 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00243290.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2487 bp

ATGAAATCTCACTTCCATTCCTCACCCCTCCCTTCACTCCGTCGCTTCTACCTCTTAAACCCCAAGCCCCTCTCCTCACAACCACCACCACCGCCGCCGCCGCAAATCCATTCTCCGGCGAACCCAATTCCACAATCCTCCGATTCAACCCAAACCCAACTCCTCAACACTCTTCTTTCCCACAAGACCGATCCCAGTTCCGCTCTCGACCTCCTCAAACGCGCTGAAGCTCACCCAGGCTTTGTCCACGGCGCCGACACCGTCGCCGTCTTGCTTCACATCTTAATGGGCTTTCCAGAGACTCGTGGGCGTGCCAAGACATTGCTTAACCGCTACGTTTCCGGCGACTCCGGCCCGCCTCCGGCAGTCTTTCTCGATCGTTTGCTGGACTGCGCAAAGAGGTTTGGTTTTGATTTTGATTCAGATTCTCTGGTTTTCAGTTACTTGTTGAATAGCTATGTTAGAGCGAATCGAATAGGGGATGCTGTTGATTGCTTTAATAGAATGGTGGAGCTTGAGATATATGCTAGTGTTGAATATATGAACATTGTGTTGACGGCATTGGTTAGGAGGAATATGTTTGAGGAAGCACGAGAGTTGTATGGTAAAATGGTTGAGAGAGGAGTTGGCGGTGATTGCGCCACTCTACATGTCATGATGCGTGCTTGTTTGAAGGAGGGGAAGCCGGAGGGGGCCGAGGACTACTTTCGGAAGGCAAGGGGGAGAGGGATAGCAGCTGATGCGGCATTGTATGATGTGGCAATTGGGGCTGTTTGTAAGACTCCCGAATCGCGTTTGGCTTTGGTGTTGTTGGGTGAAATGAGAGAAATGGGGTGGGTTCCTTCCGGGCGTACATATACTAGTGTGATTGGGGCGTGTGTGAAGCAGGGGAATATGGTGGAGGCGCTAAGGCTCAAGGATGAAATGGTGAGTTGTGGGGAATCGATTGATTTGGTTGTTGCTACAAGCTTGATGAAGGGTTATTGTGCGCAAGGGAAGTTGGATGCTGCTTTGGATTTGTTCAACATGATTATGCAGGATGGACTTATTCCTAACAGAATCACTTATGCAACTGTGATAGGATGCTGCTGTAAGAATGAGAACATGGAAAGGGCATATGAGCTTTACACCAAAATGAAACAGATGGGTATTTTGCCTGATGAGTTCATTGTGAATCATTTGGTGCGTGGATTTTTGAAATGTCGGTTACTTGAAGATGCGTCTAAGTTGTTTGACGAGGCAGTTGACTACGGTATTGCAAATGTCTTCTTGTACAATAATTTCTTAGCATGGCTTTGTAGGGAGGGTAAAGTAAGCGAAGCCCGTAGTTTATGGGATAAGATGATGCGTAATGGTGTGATACCTAATGCAGTTTCCTACAACAACATGATACATGGCTATTGCAGAATAGGGGATATGGATTGTGCAAATAATGAATTTTTGGAGATGACACAAAGGGGTTTGGAACCTACTGTTTTTACTTACTCTATTTTGATGAATGGGTATTTCAGGAACGGTAATGCCGAACGTGCTTTTAATGTCTTTGATGATATGGTGGATGCAAAGAAAATCCCCACAGGCTACACAATTAACATTGTCATTGATGGCCTGTGCAAAGTTGGCCAGACATCTGTGGCAAGTGATCTTTTGAAGAAATTTCTTGAGAAGGGTTTTATTCCTGGATGTATGACTTATAATAGTATTATACATGGGTTTTCCAGGGAGGGTGCCATGAATTCTGCACTGAATGTTTATAGAGAAATGTGTGAAGGTGGAGTCACTCCAACTGTTATCACTTACACCAGCCTTATTAATGGGTTATGCAAAAGCAATAAGATAGATCTTGCTTTAGAAATGTGGCATGAAATGAAAAGTAAGGGTATCAAAATGGATGTTAAAGCATACTCTGCTATCATTGATGGGTTTTGCAAAAGGCGAGACTTGAAAAGTGCCCGCGAACTTTTCTCGGAACTTCTGGAAGTTGGTTTATCTCCAAATACAGGTGTTTATACTAGCATGATTTGTGGCTTCCGGAATGTAAATGACATGGAAGCAGCTATTGAGTTACATAAGAAAATGATAACTGAAGGCATTCCATGTGATGTGAAAACGTACACTGCATTAATCAATGGACTCCTAAGAAAGGGTGATTTACTATCTGCATCAGATCTTTACTCAAAGATGCTTTCGAAGGGAATTACACCTGATATCACCACATATACAGTTCTAATAAATGGCCTTTGTTGCAAACGACAATTGGAGAAGGCACGCAAAACCTTGGAATACATGAATAGAAGGAGAATGACTGCTAGGGTTCATATTTATACTGCTTTGATTGTTGGGCACTTCAAGGAGGGGAATTTGCAAGAATCGTTTAGGTTGCACGATGAAATGCTAGACAAAGGCCTCGTACCTGATGACATTACTTGTGATATTCTTATAAATGGAAAGTTCAAAGGCATAAGTGCTCTTCCGAGGACTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

828

Amino Acids

92.36

Weight (kDa)

8.03

Isoelectric Point (pI)

31.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	180 - 225	4.9e-07	PPR repeat family
PPR	PF01535	285 - 313	6.7e-06	PPR repeat
PPR_3	PF13812	304 - 366	1.7e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	317 - 365	2.6e-11	PPR repeat family
PPR_1	PF12854	347 - 380	3.8e-07	PPR repeat
PPR	PF01535	354 - 384	4.9e-06	PPR repeat
PPR_2	PF13041	354 - 389	5.1e-08	PPR repeat family
PPR_2	PF13041	421 - 461	7e-11	PPR repeat family
PPR_long	PF17177	429 - 542	5.6e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	444 - 501	5.4e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	451 - 477	2.6e-07	PPR repeat
PPR_2	PF13041	455 - 502	6.7e-16	PPR repeat family
PPR_3	PF13812	482 - 534	3.9e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	486 - 519	6e-11	PPR repeat
PPR	PF01535	493 - 520	2.6e-06	PPR repeat
PPR_2	PF13041	497 - 539	1.2e-10	PPR repeat family
TPR_24	PF23276	539 - 657	1.9e-10	Fungal tetratrico peptide repeats
PPR_3	PF13812	549 - 605	3.4e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	556 - 588	3.8e-06	PPR repeat
PPR_2	PF13041	563 - 609	1.1e-16	PPR repeat family
PPR_long	PF17177	571 - 716	2.3e-11	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	592 - 624	6.5e-12	PPR repeat
PPR_2	PF13041	597 - 644	7.3e-17	PPR repeat family
PPR	PF01535	598 - 628	3.2e-09	PPR repeat
PPR_3	PF13812	620 - 674	8.5e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	626 - 657	1.5e-06	PPR repeat
PPR_2	PF13041	631 - 677	1.3e-09	PPR repeat family
PPR_2	PF13041	665 - 713	1.8e-11	PPR repeat family
PPR_3	PF13812	690 - 745	3.6e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	701 - 748	4.1e-16	PPR repeat family
PPR_1	PF12854	731 - 763	4.5e-08	PPR repeat
PPR_2	PF13041	772 - 816	3.4e-10	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0002613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39230 AT3G54980 AT3G54980
fragaria_vesca	FvH4_5g25890 FvH4_5g25890 FvH4_5g25890
malus_domestica	MD04G1151000.v1.1 MD12G1164900.v1.1
prunus_persica	Prupe.6G275900_v2.0.a1 Prupe.6G275900_v2.0.a1
pyrus_communis	pycom04g13670 pycom12g15770
rosa_chinensis	RchiOBHm_Chr4g0410561 RchiOBHm_Chr4g0410571 RchiOBHm_Chr5g0071501 RchiOBHm_Chr6g0264781 RchiOBHm_Chr7g0216391 RchiOBHm_Chr7g0216401
rosa_laevigata	RLG00000002575 RLG00000002576 RLG00000002577 RLG00000031675
rosa_multiflora	Rmu_sc0000692.1_g000003 Rmu_sc0000692.1_g000005 Rmu_sc0006325.1_g000046
rosa_roxburghii	Rroxscaffold_159G00432870 Rroxscaffold_3G00243290 Rroxscaffold_4G00298080
rosa_rugosa	Rorug01G0081600 Rorug07G0160300
rosa_samantha	Rh2DG118300 Rh4CG174000 Rh4CG174100 Rh4CG174200 Rh7AG298700 Rh7AG298800 Rh7BG290300 Rh7BG290400
rosa_wichuraiana	Rw7G025370 Rw7G025380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1695, 2441
Acc16I	TGCGCA	2 cut(s)	394, 990
AccB1I	GGYRCC	2 cut(s)	251, 1727
AccBSI	CCGCTC	1 cut(s)	203
AccII	CGCG	3 cut(s)	222, 793, 1956
AccIII	TCCGGA	1 cut(s)	2031
AclWI	GGATC	1 cut(s)	185
AcoI	YGGCCR	1 cut(s)	1621
AcsI	RAATTY	4 cut(s)	1436, 1655, 1735, 2359
AcuI	CTGAAG	2 cut(s)	246, 2106
AcyI	GRCGYC	1 cut(s)	252
AfaI	GTAC	4 cut(s)	853, 1274, 2114, 2412
AfiI	CCNNNNNNNGG	4 cut(s)	115, 361, 2000, 2143
AflIII	ACRYGT	1 cut(s)	649
AhlI	ACTAGT	1 cut(s)	860
AjnI	CCWGG	3 cut(s)	235, 1678, 1718
AluBI	AGCT	7 cut(s)	230, 456, 514, 737, 969, 1123, 2057
AluI	AGCT	7 cut(s)	230, 456, 514, 737, 969, 1123, 2057
Alw21I	GWGCWC	1 cut(s)	2470
Alw26I	GTCTC	2 cut(s)	293, 1932
AlwI	GGATC	1 cut(s)	185
Aor13HI	TCCGGA	1 cut(s)	2031
AoxI	GGCC	6 cut(s)	352, 693, 1606, 1621, 2236, 2404
ApeKI	GCWGC	4 cut(s)	734, 1007, 1086, 2054
ApoI	RAATTY	4 cut(s)	1436, 1655, 1735, 2359
AseI	ATTAAT	2 cut(s)	1812, 2123
Asp700I	GAANNNNTTC	1 cut(s)	2091
AspLEI	GCGC	6 cut(s)	224, 254, 395, 641, 907, 991
AspS9I	GGNCC	2 cut(s)	353, 693
AsuC2I	CCSGG	1 cut(s)	847
AsuHPI	GGTGA	7 cut(s)	15, 224, 644, 827, 940, 2159, 2203
AsuII	TTCGAA	1 cut(s)	2189
BaeGI	GKGCMC	2 cut(s)	1954, 2346
BalI	TGGCCA	1 cut(s)	1623
BanI	GGYRCC	2 cut(s)	251, 1727
BauI	CACGAG	2 cut(s)	303, 591
BbsI	GAAGAC	1 cut(s)	1258
Bbv12I	GWGCWC	1 cut(s)	2470
BbvI	GCAGC	4 cut(s)	746, 994, 1073, 2066
BccI	CCATC	4 cut(s)	1034, 1138, 1598, 1913
BceAI	ACGGC	3 cut(s)	251, 265, 573
BciT130I	CCWGG	3 cut(s)	237, 1680, 1720
BcnI	CCSGG	1 cut(s)	847
BcoDI	GTCTC	2 cut(s)	293, 1932
BcuI	ACTAGT	1 cut(s)	860
BfaI	CTAG	5 cut(s)	528, 861, 2013, 2310, 2396
BfoI	RGCGCY	2 cut(s)	255, 908
BglII	AGATCT	2 cut(s)	1840, 2167
BisI	GCNGC	8 cut(s)	95, 98, 101, 735, 744, 1008, 1087, 2055
BlsI	GCNGC	8 cut(s)	96, 99, 102, 736, 745, 1009, 1088, 2056
Bme1390I	CCNGG	4 cut(s)	237, 847, 1680, 1720
BmgT120I	GGNCC	2 cut(s)	353, 693
BmiI	GGNNCC	5 cut(s)	253, 694, 840, 1467, 1729
BmrFI	CCNGG	4 cut(s)	237, 847, 1680, 1720
BmrI	ACTGGG	1 cut(s)	189
BmuI	ACTGGG	1 cut(s)	189
BpiI	GAAGAC	1 cut(s)	1258
Bpu10I	CCTNAGC	1 cut(s)	908
Bpu14I	TTCGAA	1 cut(s)	2189
BpuEI	CTTGAG	3 cut(s)	536, 899, 1682
BpuMI	CCSGG	1 cut(s)	847
Bsa29I	ATCGAT	1 cut(s)	944
BsaHI	GRCGYC	1 cut(s)	252
BsaJI	CCNNGG	6 cut(s)	235, 247, 696, 1719, 2277, 2474
BsaWI	WCCGGW	1 cut(s)	2031
Bsc4I	CCNNNNNNNGG	4 cut(s)	115, 361, 2000, 2143
Bse1I	ACTGG	1 cut(s)	195
Bse3DI	GCAATG	1 cut(s)	320
BseAI	TCCGGA	1 cut(s)	2031
BseBI	CCWGG	3 cut(s)	237, 1680, 1720
BseCI	ATCGAT	1 cut(s)	944
BseDI	CCNNGG	6 cut(s)	235, 247, 696, 1719, 2277, 2474
BseGI	GGATG	8 cut(s)	487, 925, 1009, 1045, 1088, 1564, 1688, 1897
BseLI	CCNNNNNNNGG	4 cut(s)	115, 361, 2000, 2143
BseMI	GCAATG	1 cut(s)	320
BseNI	ACTGG	1 cut(s)	195
BseRI	GAGGAG	4 cut(s)	64, 152, 203, 636
BseSI	GKGCMC	2 cut(s)	1954, 2346
BseXI	GCAGC	4 cut(s)	746, 994, 1073, 2066
BsgI	GTGCAG	1 cut(s)	1725
Bsh1236I	CGCG	3 cut(s)	222, 793, 1956
Bsh1285I	CGRYCG	1 cut(s)	379
BshFI	GGCC	6 cut(s)	354, 695, 1608, 1623, 2238, 2406
BshNI	GGYRCC	2 cut(s)	251, 1727
BshVI	ATCGAT	1 cut(s)	944
BsiEI	CGRYCG	1 cut(s)	379
BsiHKAI	GWGCWC	1 cut(s)	2470
BsiSI	CCGG	7 cut(s)	116, 342, 351, 362, 686, 846, 2032
BslI	CCNNNNNNNGG	4 cut(s)	115, 361, 2000, 2143
BsmAI	GTCTC	2 cut(s)	293, 1932
BsmI	GAATGC	1 cut(s)	2091
BsnI	GGCC	6 cut(s)	354, 695, 1608, 1623, 2238, 2406
Bsp119I	TTCGAA	1 cut(s)	2189
Bsp1286I	GDGCHC	3 cut(s)	1954, 2346, 2470
Bsp13I	TCCGGA	1 cut(s)	2031
Bsp1407I	TGTACA	1 cut(s)	1272
Bsp143I	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
BspANI	GGCC	6 cut(s)	354, 695, 1608, 1623, 2238, 2406
BspDI	ATCGAT	1 cut(s)	944
BspEI	TCCGGA	1 cut(s)	2031
BspFNI	CGCG	3 cut(s)	222, 793, 1956
BspHI	TCATGA	2 cut(s)	654, 2483
BspLI	GGNNCC	5 cut(s)	253, 694, 840, 1467, 1729
BspPI	GGATC	1 cut(s)	185
BspQI	GCTCTTC	1 cut(s)	2475
BspT104I	TTCGAA	1 cut(s)	2189
BspT107I	GGYRCC	2 cut(s)	251, 1727
BsrBI	CCGCTC	1 cut(s)	203
BsrDI	GCAATG	1 cut(s)	320
BsrGI	TGTACA	1 cut(s)	1272
BsrI	ACTGG	1 cut(s)	195
BssECI	CCNNGG	6 cut(s)	235, 247, 696, 1719, 2277, 2474
BssMI	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
BssNI	GRCGYC	1 cut(s)	252
BssSI	CACGAG	2 cut(s)	303, 591
BssT1I	CCWWGG	1 cut(s)	2277
Bst2BI	CACGAG	2 cut(s)	303, 591
Bst2UI	CCWGG	3 cut(s)	237, 1680, 1720
Bst4CI	ACNGT	7 cut(s)	262, 1075, 1253, 1474, 1517, 1789, 2224
Bst6I	CTCTTC	2 cut(s)	177, 2475
BstACI	GRCGYC	1 cut(s)	252
BstAPI	GCANNNNNTGC	2 cut(s)	740, 1907
BstAUI	TGTACA	1 cut(s)	1272
BstBI	TTCGAA	1 cut(s)	2189
BstC8I	GCNNGC	5 cut(s)	312, 356, 663, 1954, 2269
BstDEI	CTNAG	4 cut(s)	908, 1224, 1285, 2138
BstDSI	CCRYGG	1 cut(s)	247
BstENI	CCTNNNNNAGG	1 cut(s)	2141
BstF5I	GGATG	8 cut(s)	487, 925, 1009, 1045, 1088, 1564, 1688, 1897
BstFNI	CGCG	3 cut(s)	222, 793, 1956
BstH2I	RGCGCY	2 cut(s)	255, 908
BstHHI	GCGC	6 cut(s)	224, 254, 395, 641, 907, 991
BstKTI	GATC	5 cut(s)	193, 379, 1645, 1843, 2170
BstMAI	GTCTC	2 cut(s)	293, 1932
BstMBI	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
BstMCI	CGRYCG	1 cut(s)	379
BstNI	CCWGG	3 cut(s)	237, 1680, 1720
BstNSI	RCATGY	1 cut(s)	653
BstSCI	CCNGG	4 cut(s)	235, 845, 1678, 1718
BstSLI	GKGCMC	2 cut(s)	1954, 2346
BstUI	CGCG	3 cut(s)	222, 793, 1956
BstV1I	GCAGC	4 cut(s)	746, 994, 1073, 2066
BstV2I	GAAGAC	1 cut(s)	1258
BstX2I	RGATCY	2 cut(s)	1840, 2167
BstYI	RGATCY	2 cut(s)	1840, 2167
Bsu15I	ATCGAT	1 cut(s)	944
BsuRI	GGCC	6 cut(s)	354, 695, 1608, 1623, 2238, 2406
BsuTUI	ATCGAT	1 cut(s)	944
BtgI	CCRYGG	1 cut(s)	247
BtsCI	GGATG	8 cut(s)	487, 925, 1009, 1045, 1088, 1564, 1688, 1897
BtsI	GCAGTG	1 cut(s)	2115
BtsIMutI	CAGTG	2 cut(s)	1742, 2115
Cac8I	GCNNGC	5 cut(s)	312, 356, 663, 1954, 2269
CciI	TCATGA	2 cut(s)	654, 2483
CfoI	GCGC	6 cut(s)	224, 254, 395, 641, 907, 991
Cfr13I	GGNCC	2 cut(s)	353, 693
ClaI	ATCGAT	1 cut(s)	944
CseI	GACGC	1 cut(s)	1209
Csp6I	GTAC	4 cut(s)	852, 1273, 2113, 2411
CviQI	GTAC	4 cut(s)	852, 1273, 2113, 2411
DdeI	CTNAG	4 cut(s)	908, 1224, 1285, 2138
DinI	GGCGCC	1 cut(s)	253
DpnI	GATC	5 cut(s)	192, 378, 1644, 1842, 2169
DpnII	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
EaeI	YGGCCR	1 cut(s)	1621
Eam1104I	CTCTTC	2 cut(s)	177, 2475
EarI	CTCTTC	2 cut(s)	177, 2475
Eco130I	CCWWGG	1 cut(s)	2277
Eco147I	AGGCCT	1 cut(s)	2406
Eco32I	GATATC	1 cut(s)	2209
Eco57I	CTGAAG	2 cut(s)	246, 2106
EcoNI	CCTNNNNNAGG	1 cut(s)	2141
EcoRI	GAATTC	1 cut(s)	1735
EcoRII	CCWGG	3 cut(s)	235, 1678, 1718
EcoRV	GATATC	1 cut(s)	2209
EcoT14I	CCWWGG	1 cut(s)	2277
EgeI	GGCGCC	1 cut(s)	253
EheI	GGCGCC	1 cut(s)	253
ErhI	CCWWGG	1 cut(s)	2277
FauI	CCCGC	2 cut(s)	363, 1961
FauNDI	CATATG	1 cut(s)	1117
Fnu4HI	GCNGC	8 cut(s)	95, 98, 101, 735, 744, 1008, 1087, 2055
FokI	GGATG	8 cut(s)	494, 932, 1016, 1052, 1095, 1571, 1695, 1904
Fsp4HI	GCNGC	8 cut(s)	95, 98, 101, 735, 744, 1008, 1087, 2055
FspBI	CTAG	5 cut(s)	528, 861, 2013, 2310, 2396
FspI	TGCGCA	2 cut(s)	394, 990
GlaI	GCGC	6 cut(s)	223, 253, 394, 640, 906, 990
GluI	GCNGC	8 cut(s)	95, 98, 101, 735, 744, 1008, 1087, 2055
HaeII	RGCGCY	2 cut(s)	255, 908
HaeIII	GGCC	6 cut(s)	354, 695, 1608, 1623, 2238, 2406
HapII	CCGG	7 cut(s)	116, 342, 351, 362, 686, 846, 2032
HgaI	GACGC	1 cut(s)	1209
HhaI	GCGC	6 cut(s)	224, 254, 395, 641, 907, 991
Hin1I	GRCGYC	1 cut(s)	252
Hin6I	GCGC	6 cut(s)	222, 252, 393, 639, 905, 989
HinP1I	GCGC	6 cut(s)	222, 252, 393, 639, 905, 989
HincII	GTYRAC	2 cut(s)	555, 1246
HindII	GTYRAC	2 cut(s)	555, 1246
HindIII	AAGCTT	1 cut(s)	967
HpaII	CCGG	7 cut(s)	116, 342, 351, 362, 686, 846, 2032
HphI	GGTGA	7 cut(s)	15, 224, 644, 827, 940, 2159, 2203
Hpy166II	GTNNAC	4 cut(s)	247, 555, 1246, 2115
Hpy188I	TCNGA	6 cut(s)	142, 424, 711, 1969, 2167, 2475
Hpy8I	GTNNAC	4 cut(s)	247, 555, 1246, 2115
Hpy99I	CGWCG	2 cut(s)	45, 266
HpyCH4III	ACNGT	7 cut(s)	262, 1075, 1253, 1474, 1517, 1789, 2224
HpyCH4IV	ACGT	3 cut(s)	336, 1528, 2111
HpyF3I	CTNAG	4 cut(s)	908, 1224, 1285, 2138
HpySE526I	ACGT	3 cut(s)	336, 1528, 2111
Hsp92I	GRCGYC	1 cut(s)	252
HspAI	GCGC	6 cut(s)	222, 252, 393, 639, 905, 989
KasI	GGCGCC	1 cut(s)	251
Kpn2I	TCCGGA	1 cut(s)	2031
Kzo9I	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
LguI	GCTCTTC	1 cut(s)	2475
LmnI	GCTCC	1 cut(s)	511
Lsp1109I	GCAGC	4 cut(s)	746, 994, 1073, 2066
MaeI	CTAG	5 cut(s)	528, 861, 2013, 2310, 2396
MaeII	ACGT	3 cut(s)	336, 1528, 2111
MaeIII	GTNAC	4 cut(s)	440, 1206, 1777, 2064
MalI	GATC	5 cut(s)	192, 378, 1644, 1842, 2169
MbiI	CCGCTC	1 cut(s)	203
MboI	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
MboII	GAAGA	5 cut(s)	164, 1226, 1258, 1663, 2462
MfeI	CAATTG	2 cut(s)	762, 2255
MflI	RGATCY	2 cut(s)	1840, 2167
MhlI	GDGCHC	3 cut(s)	1954, 2346, 2470
MlsI	TGGCCA	1 cut(s)	1623
MluNI	TGGCCA	1 cut(s)	1623
Mly113I	GGCGCC	1 cut(s)	252
MlyI	GAGTC	5 cut(s)	295, 341, 775, 1785, 2127
MmeI	TCCRAC	2 cut(s)	981, 1808
Mox20I	TGGCCA	1 cut(s)	1623
MroI	TCCGGA	1 cut(s)	2031
MroXI	GAANNNNTTC	1 cut(s)	2091
MscI	TGGCCA	1 cut(s)	1623
MseI	TTAA	9 cut(s)	56, 284, 327, 498, 1536, 1590, 1812, 1896, 2123
MslI	CAYNNNNRTG	3 cut(s)	284, 1031, 1133
Msp20I	TGGCCA	1 cut(s)	1623
MspA1I	CMGCKG	1 cut(s)	737
MspI	CCGG	7 cut(s)	116, 342, 351, 362, 686, 846, 2032
MspR9I	CCNGG	4 cut(s)	237, 847, 1680, 1720
MunI	CAATTG	2 cut(s)	762, 2255
Mva1269I	GAATGC	1 cut(s)	2091
MvaI	CCWGG	3 cut(s)	237, 1680, 1720
MvnI	CGCG	3 cut(s)	222, 793, 1956
NarI	GGCGCC	1 cut(s)	252
NciI	CCSGG	1 cut(s)	847
NdeI	CATATG	1 cut(s)	1117
NdeII	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
NlaIV	GGNNCC	5 cut(s)	253, 694, 840, 1467, 1729
NmeAIII	GCCGAG	1 cut(s)	721
NmuCI	GTSAC	1 cut(s)	1777
NsbI	TGCGCA	2 cut(s)	394, 990
NspI	RCATGY	1 cut(s)	653
NspV	TTCGAA	1 cut(s)	2189
PagI	TCATGA	2 cut(s)	654, 2483
PceI	AGGCCT	1 cut(s)	2406
PciI	ACATGT	1 cut(s)	649
PciSI	GCTCTTC	1 cut(s)	2475
PcsI	WCGNNNNNNNCGW	2 cut(s)	342, 1521
PctI	GAATGC	1 cut(s)	2091
PdmI	GAANNNNTTC	1 cut(s)	2091
PfeI	GAWTC	9 cut(s)	143, 419, 425, 469, 788, 941, 1059, 1174, 2369
PflFI	GACNNNGTC	1 cut(s)	260
PfoI	TCCNGGA	1 cut(s)	1678
PkrI	GCNGC	8 cut(s)	96, 99, 102, 736, 745, 1009, 1088, 2056
Ple19I	CGATCG	1 cut(s)	379
PleI	GAGTC	5 cut(s)	295, 341, 775, 1784, 2127
PluTI	GGCGCC	1 cut(s)	255
PpsI	GAGTC	5 cut(s)	295, 341, 775, 1784, 2127
PscI	ACATGT	1 cut(s)	649
PshBI	ATTAAT	2 cut(s)	1812, 2123
PsiI	TTATAA	2 cut(s)	1695, 2441
Psp6I	CCWGG	3 cut(s)	235, 1678, 1718
PspGI	CCWGG	3 cut(s)	235, 1678, 1718
PspN4I	GGNNCC	5 cut(s)	253, 694, 840, 1467, 1729
PspPI	GGNCC	2 cut(s)	353, 693
PsuI	RGATCY	2 cut(s)	1840, 2167
PsyI	GACNNNGTC	1 cut(s)	260
PvuI	CGATCG	1 cut(s)	379
PvuII	CAGCTG	1 cut(s)	737
RsaI	GTAC	4 cut(s)	853, 1274, 2114, 2412
RsaNI	GTAC	4 cut(s)	852, 1273, 2113, 2411
RseI	CAYNNNNRTG	3 cut(s)	284, 1031, 1133
SapI	GCTCTTC	1 cut(s)	2475
SaqAI	TTAA	9 cut(s)	56, 284, 327, 498, 1536, 1590, 1812, 1896, 2123
SatI	GCNGC	8 cut(s)	95, 98, 101, 735, 744, 1008, 1087, 2055
Sau3AI	GATC	5 cut(s)	190, 376, 1642, 1840, 2167
Sau96I	GGNCC	2 cut(s)	353, 693
SchI	GAGTC	5 cut(s)	295, 341, 775, 1785, 2127
ScrFI	CCNGG	4 cut(s)	237, 847, 1680, 1720
SduI	GDGCHC	3 cut(s)	1954, 2346, 2470
SfoI	GGCGCC	1 cut(s)	253
SfuI	TTCGAA	1 cut(s)	2189
SmiMI	CAYNNNNRTG	3 cut(s)	284, 1031, 1133
SmlI	CTYRAG	3 cut(s)	515, 914, 1661
SmoI	CTYRAG	3 cut(s)	515, 914, 1661
SpeI	ACTAGT	1 cut(s)	860
SseBI	AGGCCT	1 cut(s)	2406
SspDI	GGCGCC	1 cut(s)	251
SspMI	CTAG	5 cut(s)	528, 861, 2013, 2310, 2396
StuI	AGGCCT	1 cut(s)	2406
StyD4I	CCNGG	4 cut(s)	235, 845, 1678, 1718
StyI	CCWWGG	1 cut(s)	2277
TaaI	ACNGT	7 cut(s)	262, 1075, 1253, 1474, 1517, 1789, 2224
TaiI	ACGT	3 cut(s)	339, 1531, 2114
TaqI	TCGA	5 cut(s)	207, 375, 472, 944, 2189
TaqII	GACCGA	1 cut(s)	203
TatI	WGTACW	1 cut(s)	1272
TauI	GCSGC	4 cut(s)	97, 100, 103, 746
TfiI	GAWTC	9 cut(s)	143, 419, 425, 469, 788, 941, 1059, 1174, 2369
Tru1I	TTAA	9 cut(s)	56, 284, 327, 498, 1536, 1590, 1812, 1896, 2123
Tru9I	TTAA	9 cut(s)	56, 284, 327, 498, 1536, 1590, 1812, 1896, 2123
TscAI	CASTG	2 cut(s)	1749, 2122
TseFI	GTSAC	1 cut(s)	1777
TseI	GCWGC	4 cut(s)	734, 1007, 1086, 2054
Tsp45I	GTSAC	1 cut(s)	1777
TspGWI	ACGGA	1 cut(s)	29
TspRI	CASTG	2 cut(s)	1749, 2122
Tth111I	GACNNNGTC	1 cut(s)	260
VspI	ATTAAT	2 cut(s)	1812, 2123
XagI	CCTNNNNNAGG	1 cut(s)	2141
XapI	RAATTY	4 cut(s)	1436, 1655, 1735, 2359
XceI	RCATGY	1 cut(s)	653
XmnI	GAANNNNTTC	1 cut(s)	2091
XspI	CTAG	5 cut(s)	528, 861, 2013, 2310, 2396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.