AT3G55080

Rubisco LSMT substrate-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000846
Physical Location & Seq
Reverse (-)
20411342 .. 20416043
4702 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G55080.5

Sequence Viewer

Length: 1392 bp
ATGCTATTCTGCATTTCGACAGTGAAGCTTTTCGGGTTTCAACAAAGGCGCAATGTTAGCTCTTTAGCCAAGCGTTTCAGTCTCGCCGGGAAACTAACGTTAGAGCTCCAAACTCAGGCTTCGTTGGACAATAACTTCTTACCCTGGTTAGAGCGAATTGCTGGAGCAAAGATAACTAATACTCTTTCGATTGGGAAATCCACCTATGGCAGGTCACTGTTCGCCTCCAAAGTTATCTATGCTGGTGACTGCATGTTGAAAGTCCCTTTTAATGCGCAAATAACTCCAGATGAGCTCCCCTCGGATATTAGAGTTTTATTGAGCAATGAAGTTGGGAATATCGGAATGCTTGCTGCTGTTCTAATAAGAGAGAAGAAAATGGGTCAAAAGTCTCGATGGGTCCCTTATATCAGCCGACTTCCTCAACCTGCAGAGATGCATAGCTCGATATTTTGGGGTGAAGATGAGCTCAGCATGATTCGTTGTAGTGCAGTTCACCAGGAAACAGTAAAACAAAAAGCTCAGATAGAAAAGGACTTTTCATTTGTAGCGCAGGCATTCAAACAACATTGTCCCATAGTAACTGAACGCCCAGATTTGGAAGATTTCATGTATGCATATGCTTTAGTGGGATCTCGTGCGTGGGAAAACTCAAAACGCATATCACTGATTCCGTTTGCAGACTTCATGAACCATGATGGGCTTTCTGCATCAATCGTTTTGAGGGATGAAGACAATCAACTATCAGAGGTCACTGCAGATCGCAACTACAGTCCTGGTGATGAGGTATTCATCAAGTATGGGGAATTCTCAAATGCTACACTCATGTTAGATTTTGGATTTACCTTTCCTTACAACATTCATGACGAGGTTCAAATCCAAATGGATGTACCGAATGATGATCCTCTTCGCAACATGAAGCTGGGACTTCTCCAGACACATCACACTCGAACTGTCAAAGATATCAATATCTTTCACTCTTCTTGTGATACTTTCACAATCAAGGAAGTGAAATCTGCTATAGGGAAAGGAAAAGGCATTCCACAATCTCTCCGTGCATTTGCTCGTGTTCTCTGTTGTATCATTCCCCAAGAGCTAAATGATTTGTCAAAAGAAGCTGCACAAAATGATGGCCGACTAGCTCGGCTTCCTTTTAAAGACGGGAACAGAGAGTTGGAGGCACATAAGATCCTCTTGTCTCACATCAACAGATTGATTGAGGATCATAGTGTTTGCATCAAGGAAATGGAAGAATGCTATTTTGTATCACAAAGATTTGCTGTTAGAAGGCAGATGGCAAGAGATCTCCTATACGGTGAGCTTCGTGTACTGAGATCTGCAGCTGAATGGCTCAATCATTATTGCACAACCTTGCTTTCAGAGACAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

463

Amino Acids

52.69

Weight (kDa)

6.87

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rubis-subs-bind PF09273 215 - 360 3.7e-07 Rubisco LSMT substrate-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 276
Acc36I ACCTGC 2 cut(s) 201, 436
AclI AACGTT 1 cut(s) 98
AclWI GGATC 4 cut(s) 640, 896, 1183, 1230
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 1 cut(s) 806
AfaI GTAC 2 cut(s) 891, 1329
AfiI CCNNNNNNNGG 3 cut(s) 115, 210, 598
AgsI TTSAA 4 cut(s) 41, 259, 562, 875
AjnI CCWGG 3 cut(s) 143, 498, 775
AjuI GAANNNNNNNTTGG 2 cut(s) 1157, 1189
Alw21I GWGCWC 3 cut(s) 108, 297, 471
Alw26I GTCTC 4 cut(s) 86, 396, 1203, 1376
AlwI GGATC 4 cut(s) 640, 896, 1183, 1230
AoxI GGCC 1 cut(s) 1132
ApeKI GCWGC 3 cut(s) 353, 1118, 1340
ApoI RAATTY 1 cut(s) 806
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 3 cut(s) 51, 277, 553
AspS9I GGNCC 1 cut(s) 400
AsuC2I CCSGG 1 cut(s) 88
AsuHPI GGTGA 5 cut(s) 257, 470, 488, 791, 1328
AvaII GGWCC 1 cut(s) 400
BanII GRGCYC 3 cut(s) 108, 297, 471
BauI CACGAG 2 cut(s) 636, 1065
BbsI GAAGAC 1 cut(s) 738
Bbv12I GWGCWC 3 cut(s) 108, 297, 471
BbvI GCAGC 3 cut(s) 340, 1105, 1352
BccI CCATC 4 cut(s) 390, 692, 1124, 1288
BciT130I CCWGG 3 cut(s) 145, 500, 777
BcnI CCSGG 1 cut(s) 88
BcoDI GTCTC 4 cut(s) 86, 396, 1203, 1376
BfaI CTAG 1 cut(s) 1139
BfmI CTRYAG 5 cut(s) 429, 756, 769, 1020, 1338
BfuAI ACCTGC 2 cut(s) 201, 436
BglII AGATCT 2 cut(s) 1303, 1334
BisI GCNGC 3 cut(s) 354, 1119, 1341
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 3 cut(s) 355, 1120, 1342
Bme1390I CCNGG 4 cut(s) 88, 145, 500, 777
Bme18I GGWCC 1 cut(s) 400
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 401, 402
BmrFI CCNGG 4 cut(s) 88, 145, 500, 777
BmsI GCATC 3 cut(s) 426, 719, 1245
BpiI GAAGAC 1 cut(s) 738
BplI GAGNNNNNCTC 2 cut(s) 284, 316
BpmI CTGGAG 3 cut(s) 183, 270, 917
Bpu1102I GCTNAGC 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 88
BsaJI CCNNGG 2 cut(s) 143, 300
BsaXI ACNNNNNCTCC 2 cut(s) 1035, 1065
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 210, 598
Bse3DI GCAATG 2 cut(s) 58, 331
BseBI CCWGG 3 cut(s) 145, 500, 777
BseDI CCNNGG 2 cut(s) 143, 300
BseGI GGATG 2 cut(s) 733, 892
BseLI CCNNNNNNNGG 3 cut(s) 115, 210, 598
BseMI GCAATG 2 cut(s) 58, 331
BseMII CTCAG 4 cut(s) 128, 484, 536, 1322
BseXI GCAGC 3 cut(s) 340, 1105, 1352
BseYI CCCAGC 1 cut(s) 922
BsgI GTGCAG 2 cut(s) 510, 1104
BshFI GGCC 1 cut(s) 1134
BsiHKAI GWGCWC 3 cut(s) 108, 297, 471
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 4 cut(s) 248, 386, 558, 939
BslI CCNNNNNNNGG 3 cut(s) 115, 210, 598
BsmAI GTCTC 4 cut(s) 86, 396, 1203, 1376
BsmFI GGGAC 4 cut(s) 248, 386, 558, 939
BsmI GAATGC 4 cut(s) 351, 557, 1038, 1259
BsnI GGCC 1 cut(s) 1134
Bsp1286I GDGCHC 3 cut(s) 108, 297, 471
Bsp143I GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
Bsp1720I GCTNAGC 1 cut(s) 470
BspANI GGCC 1 cut(s) 1134
BspCNI CTCAG 4 cut(s) 127, 483, 535, 1323
BspHI TCATGA 2 cut(s) 687, 862
BspLI GGNNCC 2 cut(s) 401, 402
BspMAI CTGCAG 3 cut(s) 433, 760, 1342
BspMI ACCTGC 2 cut(s) 201, 436
BspPI GGATC 4 cut(s) 640, 896, 1183, 1230
BsrDI GCAATG 2 cut(s) 58, 331
BssECI CCNNGG 2 cut(s) 143, 300
BssMI GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
BssSI CACGAG 2 cut(s) 636, 1065
Bst2BI CACGAG 2 cut(s) 636, 1065
Bst2UI CCWGG 3 cut(s) 145, 500, 777
Bst4CI ACNGT 6 cut(s) 22, 219, 508, 773, 955, 1316
Bst6I CTCTTC 2 cut(s) 912, 985
BstC8I GCNNGC 2 cut(s) 351, 555
BstDEI CTNAG 4 cut(s) 114, 470, 522, 1331
BstENI CCTNNNNNAGG 1 cut(s) 208
BstF5I GGATG 2 cut(s) 733, 892
BstHHI GCGC 3 cut(s) 51, 277, 553
BstKTI GATC 7 cut(s) 635, 763, 904, 1191, 1225, 1306, 1337
BstMAI GTCTC 4 cut(s) 86, 396, 1203, 1376
BstMBI GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
BstMWI GCNNNNNNNGC 1 cut(s) 57
BstNI CCWGG 3 cut(s) 145, 500, 777
BstNSI RCATGY 1 cut(s) 256
BstSCI CCNGG 4 cut(s) 86, 143, 498, 775
BstSFI CTRYAG 5 cut(s) 429, 756, 769, 1020, 1338
BstV1I GCAGC 3 cut(s) 340, 1105, 1352
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 4 cut(s) 632, 1188, 1303, 1334
BstYI RGATCY 4 cut(s) 632, 1188, 1303, 1334
BsuRI GGCC 1 cut(s) 1134
BtsCI GGATG 2 cut(s) 733, 892
BtsI GCAGTG 1 cut(s) 753
BtsIMutI CAGTG 4 cut(s) 27, 215, 665, 753
BveI ACCTGC 2 cut(s) 201, 436
Cac8I GCNNGC 2 cut(s) 351, 555
CciI TCATGA 2 cut(s) 687, 862
CfoI GCGC 3 cut(s) 51, 277, 553
Cfr13I GGNCC 1 cut(s) 400
Csp6I GTAC 2 cut(s) 890, 1328
CviAII CATG 8 cut(s) 253, 475, 610, 688, 695, 826, 863, 916
CviQI GTAC 2 cut(s) 890, 1328
DdeI CTNAG 4 cut(s) 114, 470, 522, 1331
DpnI GATC 7 cut(s) 634, 762, 903, 1190, 1224, 1305, 1336
DpnII GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
DraI TTTAAA 1 cut(s) 1156
EaeI YGGCCR 1 cut(s) 1132
Eam1104I CTCTTC 2 cut(s) 912, 985
EarI CTCTTC 2 cut(s) 912, 985
Ecl136II GAGCTC 3 cut(s) 106, 295, 469
Eco24I GRGCYC 3 cut(s) 108, 297, 471
Eco32I GATATC 1 cut(s) 964
Eco47I GGWCC 1 cut(s) 400
Eco53kI GAGCTC 3 cut(s) 106, 295, 469
EcoICRI GAGCTC 3 cut(s) 106, 295, 469
EcoNI CCTNNNNNAGG 1 cut(s) 208
EcoO109I RGGNCCY 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 806
EcoRII CCWGG 3 cut(s) 143, 498, 775
EcoRV GATATC 1 cut(s) 964
EcoT22I ATGCAT 2 cut(s) 441, 619
EcoT38I GRGCYC 3 cut(s) 108, 297, 471
FaeI CATG 8 cut(s) 256, 478, 613, 691, 698, 829, 866, 919
FalI AAGNNNNNCTT 2 cut(s) 1178, 1210
FaqI GGGAC 4 cut(s) 248, 386, 558, 939
FatI CATG 8 cut(s) 252, 474, 609, 687, 694, 825, 862, 915
FauNDI CATATG 1 cut(s) 619
Fnu4HI GCNGC 3 cut(s) 354, 1119, 1341
FokI GGATG 2 cut(s) 740, 899
FriOI GRGCYC 3 cut(s) 108, 297, 471
Fsp4HI GCNGC 3 cut(s) 354, 1119, 1341
FspBI CTAG 1 cut(s) 1139
FspI TGCGCA 1 cut(s) 276
GlaI GCGC 3 cut(s) 50, 276, 552
GluI GCNGC 3 cut(s) 354, 1119, 1341
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 3 cut(s) 183, 270, 917
HaeIII GGCC 1 cut(s) 1134
HapII CCGG 1 cut(s) 87
HhaI GCGC 3 cut(s) 51, 277, 553
Hin1II CATG 8 cut(s) 256, 478, 613, 691, 698, 829, 866, 919
Hin6I GCGC 3 cut(s) 49, 275, 551
HinP1I GCGC 3 cut(s) 49, 275, 551
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 2 cut(s) 478, 670
HpaII CCGG 1 cut(s) 87
HphI GGTGA 5 cut(s) 257, 470, 488, 791, 1328
Hpy166II GTNNAC 2 cut(s) 496, 1328
Hpy188I TCNGA 5 cut(s) 304, 344, 525, 748, 1381
Hpy188III TCNNGA 5 cut(s) 287, 393, 688, 863, 934
Hpy8I GTNNAC 2 cut(s) 496, 1328
HpyAV CCTTC 1 cut(s) 1281
HpyCH4III ACNGT 6 cut(s) 22, 219, 508, 773, 955, 1316
HpyCH4IV ACGT 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 1 cut(s) 57
HpyF3I CTNAG 4 cut(s) 114, 470, 522, 1331
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 8 cut(s) 256, 478, 613, 691, 698, 829, 866, 919
HspAI GCGC 3 cut(s) 49, 275, 551
KflI GGGWCCC 1 cut(s) 400
Kzo9I GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
LmnI GCTCC 3 cut(s) 111, 164, 300
Lsp1109I GCAGC 3 cut(s) 340, 1105, 1352
LweI GCATC 3 cut(s) 426, 719, 1245
MaeI CTAG 1 cut(s) 1139
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 4 cut(s) 213, 245, 580, 751
MalI GATC 7 cut(s) 634, 762, 903, 1190, 1224, 1305, 1336
MboI GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
MboII GAAGA 7 cut(s) 385, 473, 614, 743, 899, 972, 1262
MflI RGATCY 4 cut(s) 632, 1188, 1303, 1334
MhlI GDGCHC 3 cut(s) 108, 297, 471
MluCI AATT 2 cut(s) 156, 806
MmeI TCCRAC 2 cut(s) 105, 1155
Mph1103I ATGCAT 2 cut(s) 441, 619
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 2 cut(s) 270, 1155
MspA1I CMGCKG 1 cut(s) 1343
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 4 cut(s) 88, 145, 500, 777
Mva1269I GAATGC 4 cut(s) 351, 557, 1038, 1259
MvaI CCWGG 3 cut(s) 145, 500, 777
MwoI GCNNNNNNNGC 1 cut(s) 57
NciI CCSGG 1 cut(s) 88
NdeI CATATG 1 cut(s) 619
NdeII GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
NlaIII CATG 8 cut(s) 256, 478, 613, 691, 698, 829, 866, 919
NlaIV GGNNCC 2 cut(s) 401, 402
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 3 cut(s) 213, 245, 751
NsbI TGCGCA 1 cut(s) 276
NsiI ATGCAT 2 cut(s) 441, 619
NspI RCATGY 1 cut(s) 256
PagI TCATGA 2 cut(s) 687, 862
PctI GAATGC 4 cut(s) 351, 557, 1038, 1259
PdmI GAANNNNTTC 1 cut(s) 29
PfeI GAWTC 2 cut(s) 478, 670
PkrI GCNGC 3 cut(s) 355, 1120, 1342
PpuMI RGGWCCY 1 cut(s) 400
Psp124BI GAGCTC 3 cut(s) 108, 297, 471
Psp1406I AACGTT 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 143, 498, 775
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 3 cut(s) 143, 498, 775
PspN4I GGNNCC 2 cut(s) 401, 402
PspPI GGNCC 1 cut(s) 400
PspPPI RGGWCCY 1 cut(s) 400
PstI CTGCAG 3 cut(s) 433, 760, 1342
PsuI RGATCY 4 cut(s) 632, 1188, 1303, 1334
PvuII CAGCTG 1 cut(s) 1343
RsaI GTAC 2 cut(s) 891, 1329
RsaNI GTAC 2 cut(s) 890, 1328
SacI GAGCTC 3 cut(s) 108, 297, 471
SaqAI TTAA 2 cut(s) 270, 1155
SatI GCNGC 3 cut(s) 354, 1119, 1341
Sau3AI GATC 7 cut(s) 632, 760, 901, 1188, 1222, 1303, 1334
Sau96I GGNCC 1 cut(s) 400
ScrFI CCNGG 4 cut(s) 88, 145, 500, 777
SduI GDGCHC 3 cut(s) 108, 297, 471
SfaNI GCATC 3 cut(s) 426, 719, 1245
SfcI CTRYAG 5 cut(s) 429, 756, 769, 1020, 1338
SinI GGWCC 1 cut(s) 400
Sse9I AATT 2 cut(s) 156, 806
SspMI CTAG 1 cut(s) 1139
SstI GAGCTC 3 cut(s) 108, 297, 471
StyD4I CCNGG 4 cut(s) 86, 143, 498, 775
TaaI ACNGT 6 cut(s) 22, 219, 508, 773, 955, 1316
TaiI ACGT 1 cut(s) 101
TaqI TCGA 5 cut(s) 17, 188, 394, 446, 949
TasI AATT 2 cut(s) 156, 806
TatI WGTACW 1 cut(s) 1327
TfiI GAWTC 2 cut(s) 478, 670
Tru1I TTAA 2 cut(s) 270, 1155
Tru9I TTAA 2 cut(s) 270, 1155
TscAI CASTG 4 cut(s) 27, 222, 672, 760
TseFI GTSAC 3 cut(s) 213, 245, 751
TseI GCWGC 3 cut(s) 353, 1118, 1340
Tsp45I GTSAC 3 cut(s) 213, 245, 751
TspDTI ATGAA 9 cut(s) 342, 531, 598, 676, 704, 744, 781, 851, 932
TspGWI ACGGA 2 cut(s) 663, 1043
TspRI CASTG 4 cut(s) 27, 222, 672, 760
VpaK11BI GGWCC 1 cut(s) 400
XagI CCTNNNNNAGG 1 cut(s) 208
XapI RAATTY 1 cut(s) 806
XceI RCATGY 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 1 cut(s) 1139
Zsp2I ATGCAT 2 cut(s) 441, 619
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.