MD01G1117100.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000846
Physical Location & Seq
Reverse (-)
23089688 .. 23092420
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1117100.v1.1.491

Sequence Viewer

Length: 474 bp
ATGGTATTGATAAGATACGGAAAATTTTCAAATGCTACCCTGCTTCTGGACTTTGGGTTTACACTTTCATACAACATCCACGACCGGGTCAGTCTTGGATTTCAGTTCACTATAGTCACAATGATTGCTTTGTTGTGTTGGGAAAATGTTTCTTGGGAACTTTTGAAGAGACATCACAGACCAATCAGTAATGATGTCAATGGTTTCGACTCTTCAATGGATTCTTTCACTATCAAGTTGGTAGTGATGCACTTTAGGTCCGGTAGCCGAAAAGGGAGAGGAATTCCTCAGTCACTTGGTGCTTTTGCTCGTGTTCTATGTTGCGCCTCTCCTCAAGTTACATGTGAAAGACTTTTTATCCGGAGGCAAATGGCTCAGGATCTCCTCAATGGTGAACATCATATCCTCAAATCTGCTTCTGCGTGGCTAAGAAACTACTGCGACACTTTGACTACAACAGACTGCCATAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

158

Amino Acids

17.98

Weight (kDa)

9.24

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 360
AclWI GGATC 1 cut(s) 387
AcsI RAATTY 2 cut(s) 23, 282
AdeI CACNNNGTG 1 cut(s) 299
AfiI CCNNNNNNNGG 2 cut(s) 46, 85
AflIII ACRYGT 1 cut(s) 341
AgsI TTSAA 3 cut(s) 30, 166, 216
AloI GAACNNNNNNTCC 2 cut(s) 387, 419
Alw26I GTCTC 1 cut(s) 163
AlwI GGATC 1 cut(s) 387
Aor13HI TCCGGA 1 cut(s) 360
ApoI RAATTY 2 cut(s) 23, 282
Asp700I GAANNNNTTC 1 cut(s) 25
AspLEI GCGC 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 258
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 1 cut(s) 404
AvaII GGWCC 1 cut(s) 258
BauI CACGAG 1 cut(s) 309
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 163
BfmI CTRYAG 1 cut(s) 111
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 258
BmgT120I GGNCC 1 cut(s) 258
BmrFI CCNGG 1 cut(s) 86
BmsI GCATC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 86
BsaWI WCCGGW 2 cut(s) 260, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 85
BseAI TCCGGA 1 cut(s) 360
BseGI GGATG 1 cut(s) 75
BseLI CCNNNNNNNGG 2 cut(s) 46, 85
BseMII CTCAG 2 cut(s) 302, 389
BseRI GAGGAG 2 cut(s) 321, 374
Bsh1285I CGRYCG 1 cut(s) 85
BsiEI CGRYCG 1 cut(s) 85
BsiSI CCGG 3 cut(s) 85, 261, 361
BslI CCNNNNNNNGG 2 cut(s) 46, 85
BsmAI GTCTC 1 cut(s) 163
Bsp13I TCCGGA 1 cut(s) 360
Bsp143I GATC 1 cut(s) 379
BspCNI CTCAG 2 cut(s) 301, 388
BspEI TCCGGA 1 cut(s) 360
BspPI GGATC 1 cut(s) 387
BssMI GATC 1 cut(s) 379
BssSI CACGAG 1 cut(s) 309
Bst2BI CACGAG 1 cut(s) 309
Bst6I CTCTTC 2 cut(s) 161, 217
BstDEI CTNAG 3 cut(s) 288, 375, 428
BstF5I GGATG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 326
BstKTI GATC 1 cut(s) 382
BstMAI GTCTC 1 cut(s) 163
BstMBI GATC 1 cut(s) 379
BstMCI CGRYCG 1 cut(s) 85
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 84
BstSFI CTRYAG 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BtsCI GGATG 1 cut(s) 75
CfoI GCGC 1 cut(s) 326
Cfr13I GGNCC 1 cut(s) 258
CviAII CATG 1 cut(s) 342
CviJI RGCY 3 cut(s) 267, 374, 427
CviKI_1 RGCY 3 cut(s) 267, 374, 427
DdeI CTNAG 3 cut(s) 288, 375, 428
DpnI GATC 1 cut(s) 381
DpnII GATC 1 cut(s) 379
DraIII CACNNNGTG 1 cut(s) 299
Eam1104I CTCTTC 2 cut(s) 161, 217
EarI CTCTTC 2 cut(s) 161, 217
Eco47I GGWCC 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 282
FaeI CATG 1 cut(s) 345
FaiI YATR 6 cut(s) 70, 113, 319, 343, 402, 468
FatI CATG 1 cut(s) 341
FokI GGATG 1 cut(s) 62
GlaI GCGC 1 cut(s) 325
HapII CCGG 3 cut(s) 85, 261, 361
HhaI GCGC 1 cut(s) 326
Hin1II CATG 1 cut(s) 345
Hin6I GCGC 1 cut(s) 324
HinP1I GCGC 1 cut(s) 324
HinfI GANTC 2 cut(s) 209, 221
HpaII CCGG 3 cut(s) 85, 261, 361
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 3 cut(s) 60, 108, 395
Hpy188III TCNNGA 3 cut(s) 47, 361, 377
Hpy8I GTNNAC 3 cut(s) 60, 108, 395
HpyCH4V TGCA 1 cut(s) 250
HpyF3I CTNAG 3 cut(s) 288, 375, 428
Hsp92II CATG 1 cut(s) 345
HspAI GCGC 1 cut(s) 324
Kpn2I TCCGGA 1 cut(s) 360
Kzo9I GATC 1 cut(s) 379
LpnPI CCDG 6 cut(s) 32, 53, 98, 274, 362, 374
LweI GCATC 1 cut(s) 237
MaeIII GTNAC 3 cut(s) 115, 291, 337
MalI GATC 1 cut(s) 381
MboI GATC 1 cut(s) 379
MboII GAAGA 2 cut(s) 178, 204
MflI RGATCY 1 cut(s) 379
MluCI AATT 2 cut(s) 23, 282
MlyI GAGTC 1 cut(s) 203
MnlI CCTC 7 cut(s) 272, 297, 337, 342, 357, 395, 416
MroI TCCGGA 1 cut(s) 360
MroXI GAANNNNTTC 1 cut(s) 25
MspI CCGG 3 cut(s) 85, 261, 361
MspR9I CCNGG 1 cut(s) 86
NciI CCSGG 1 cut(s) 86
NdeII GATC 1 cut(s) 379
NlaIII CATG 1 cut(s) 345
NmuCI GTSAC 2 cut(s) 115, 291
NspI RCATGY 1 cut(s) 345
PciI ACATGT 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 25
PfeI GAWTC 1 cut(s) 221
PflFI GACNNNGTC 1 cut(s) 86
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PscI ACATGT 1 cut(s) 341
PspPI GGNCC 1 cut(s) 258
PsuI RGATCY 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 86
Sau3AI GATC 1 cut(s) 379
Sau96I GGNCC 1 cut(s) 258
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 86
SetI ASST 1 cut(s) 260
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 111
SinI GGWCC 1 cut(s) 258
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
Sse9I AATT 2 cut(s) 23, 282
StyD4I CCNGG 1 cut(s) 84
TaqI TCGA 1 cut(s) 207
TasI AATT 2 cut(s) 23, 282
TfiI GAWTC 1 cut(s) 221
TseFI GTSAC 2 cut(s) 115, 291
Tsp45I GTSAC 2 cut(s) 115, 291
TspDTI ATGAA 1 cut(s) 57
TspGWI ACGGA 1 cut(s) 33
Tth111I GACNNNGTC 1 cut(s) 86
VpaK11BI GGWCC 1 cut(s) 258
XapI RAATTY 2 cut(s) 23, 282
XceI RCATGY 1 cut(s) 345
XmnI GAANNNNTTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.