MD01G1116300.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000846
Physical Location & Seq
Reverse (-)
22993140 .. 22995681
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1116300.v1.1.491

Sequence Viewer

Length: 345 bp
ATGGAGGCCTCGTTGCAGCAAGTAGACCAACTTGAGTGTGATTTAGGCGTTCTATTCTTTCAAGCTTTGATCAATTTTCACAAAGTGTCTGGCAAGGAGTTAAAAGTTACTTCTCGAGCATGGGGAAGCATGAAGGGTTATTCCCTGATCCCATTTGCTGATTTTCTGAATCATGACGGGACTTCAGAATCAATTGTGTTGAGTGATGACGACAAACATTTCTCAGAGGTAATTGCTGACTGCAATTATGCTCCTGGTGAACAGGTTAGACGCTACTTAGTTAAGATGGCCTGTTTCCCCTATTTAAAATTACACGTACCCTTTGGAATATCATTTTTTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

115

Amino Acids

12.95

Weight (kDa)

5.43

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 24
AclWI GGATC 1 cut(s) 142
AcuI CTGAAG 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 85
AfaI GTAC 1 cut(s) 318
AflIII ACRYGT 1 cut(s) 313
AgsI TTSAA 1 cut(s) 62
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 1 cut(s) 65
AluI AGCT 1 cut(s) 65
AlwI GGATC 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 114
AoxI GGCC 2 cut(s) 6, 288
ApeKI GCWGC 1 cut(s) 16
AsuHPI GGTGA 1 cut(s) 269
AvaI CYCGRG 1 cut(s) 114
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 280
BciT130I CCWGG 1 cut(s) 255
BclI TGATCA 1 cut(s) 69
BisI GCNGC 1 cut(s) 17
BlsI GCNGC 1 cut(s) 18
Bme1390I CCNGG 1 cut(s) 255
BmeT110I CYCGRG 1 cut(s) 114
BmrFI CCNGG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 316
BsaXI ACNNNNNCTCC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 255
BseMII CTCAG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 28
BshFI GGCC 2 cut(s) 8, 290
BsiHKCI CYCGRG 1 cut(s) 114
BslFI GGGAC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 2 cut(s) 8, 290
BsoBI CYCGRG 1 cut(s) 114
Bsp143I GATC 2 cut(s) 69, 147
BspANI GGCC 2 cut(s) 8, 290
BspCNI CTCAG 1 cut(s) 236
BspHI TCATGA 1 cut(s) 172
BspPI GGATC 1 cut(s) 142
BssMI GATC 2 cut(s) 69, 147
Bst2UI CCWGG 1 cut(s) 255
BstBAI YACGTR 1 cut(s) 316
BstDEI CTNAG 2 cut(s) 223, 277
BstKTI GATC 2 cut(s) 72, 150
BstMBI GATC 2 cut(s) 69, 147
BstNI CCWGG 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 253
BstV1I GCAGC 1 cut(s) 28
BsuRI GGCC 2 cut(s) 8, 290
CciI TCATGA 1 cut(s) 172
CseI GACGC 1 cut(s) 279
Csp6I GTAC 1 cut(s) 317
CviAII CATG 3 cut(s) 120, 130, 173
CviJI RGCY 3 cut(s) 8, 65, 290
CviKI_1 RGCY 3 cut(s) 8, 65, 290
CviQI GTAC 1 cut(s) 317
DdeI CTNAG 2 cut(s) 223, 277
DpnI GATC 2 cut(s) 71, 149
DpnII GATC 2 cut(s) 69, 147
DraI TTTAAA 1 cut(s) 306
DraIII CACNNNGTG 1 cut(s) 85
Eco147I AGGCCT 1 cut(s) 8
Eco57I CTGAAG 1 cut(s) 168
Eco88I CYCGRG 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 253
FaeI CATG 3 cut(s) 123, 133, 176
FaiI YATR 4 cut(s) 121, 131, 174, 249
FaqI GGGAC 1 cut(s) 193
FatI CATG 3 cut(s) 119, 129, 172
FbaI TGATCA 1 cut(s) 69
FblI GTMKAC 1 cut(s) 24
Fnu4HI GCNGC 1 cut(s) 17
Fsp4HI GCNGC 1 cut(s) 17
GluI GCNGC 1 cut(s) 17
HaeIII GGCC 2 cut(s) 8, 290
HgaI GACGC 1 cut(s) 279
Hin1II CATG 3 cut(s) 123, 133, 176
HindIII AAGCTT 1 cut(s) 63
HinfI GANTC 2 cut(s) 169, 188
HphI GGTGA 1 cut(s) 269
Hpy166II GTNNAC 2 cut(s) 25, 260
Hpy188I TCNGA 3 cut(s) 168, 187, 226
Hpy188III TCNNGA 2 cut(s) 114, 173
Hpy8I GTNNAC 2 cut(s) 25, 260
HpyAV CCTTC 1 cut(s) 127
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 2 cut(s) 16, 243
HpyF3I CTNAG 2 cut(s) 223, 277
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 3 cut(s) 123, 133, 176
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 2 cut(s) 69, 147
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 6 cut(s) 75, 158, 240, 248, 267, 304
Lsp1109I GCAGC 1 cut(s) 28
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 1 cut(s) 106
MalI GATC 2 cut(s) 71, 149
MboI GATC 2 cut(s) 69, 147
MfeI CAATTG 1 cut(s) 192
MluCI AATT 5 cut(s) 73, 192, 231, 244, 308
MnlI CCTC 2 cut(s) 19, 220
MseI TTAA 3 cut(s) 101, 282, 305
MspR9I CCNGG 1 cut(s) 255
MunI CAATTG 1 cut(s) 192
MvaI CCWGG 1 cut(s) 255
NdeII GATC 2 cut(s) 69, 147
NlaIII CATG 3 cut(s) 123, 133, 176
PaeR7I CTCGAG 1 cut(s) 114
PagI TCATGA 1 cut(s) 172
PceI AGGCCT 1 cut(s) 8
PfeI GAWTC 2 cut(s) 169, 188
PkrI GCNGC 1 cut(s) 18
Ppu21I YACGTR 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
RsaI GTAC 1 cut(s) 318
RsaNI GTAC 1 cut(s) 317
SaqAI TTAA 3 cut(s) 101, 282, 305
SatI GCNGC 1 cut(s) 17
Sau3AI GATC 2 cut(s) 69, 147
ScrFI CCNGG 1 cut(s) 255
SetI ASST 4 cut(s) 67, 231, 267, 318
Sfr274I CTCGAG 1 cut(s) 114
SlaI CTCGAG 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 32, 114
SmoI CTYRAG 2 cut(s) 32, 114
Sse9I AATT 5 cut(s) 73, 192, 231, 244, 308
SseBI AGGCCT 1 cut(s) 8
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 253
TaiI ACGT 1 cut(s) 318
TaqI TCGA 1 cut(s) 115
TasI AATT 5 cut(s) 73, 192, 231, 244, 308
TfiI GAWTC 2 cut(s) 169, 188
Tru1I TTAA 3 cut(s) 101, 282, 305
Tru9I TTAA 3 cut(s) 101, 282, 305
TseI GCWGC 1 cut(s) 16
TspDTI ATGAA 1 cut(s) 146
XhoI CTCGAG 1 cut(s) 114
XmiI GTMKAC 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.