MD01G1116800.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000846
Physical Location & Seq
Reverse (-)
23049591 .. 23053858
4268 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1116800.v1.1.491

Sequence Viewer

Length: 624 bp
ATGCATTGCACGATATTTTGGAGTGACAATGAGCTGGAGCTGATTCGCCAAAGCTCTGTATATCAGGAAACAATTAATCAAAGATCTCAAATCAAAAAGGAATTTTTGGTAATTAGGACGGCTCTCAAGAACTTCCCTAAAATGTCCAAGAGTATCACCTATAAGGATTTCATGCACGTATATGCTTTAGTTAGTTCTCGAGCATGGGGAAACACGAAGGGTTATTCTCTGGTCATTGCTGACCACAATTATGCTCCTGGTGAACAGGTACTGATAAGATACGGAAAATTTTCAAATGCTACCCTGCTTCGGGACTTTGGGTTTACACTTTCATACAACATCCACGACCAGGTCAATATACCTCACCATGACGTCCTTTGTGAAATGGAGTTGGAACTTTTGAAGAGAGATCACAGACCAATCAGTAATGCTGTCAATGGTTTTGACTCTTCAATGGATTCTTTCACTATCAAGTTGTTAGGTCTGGTAGTGGAAAAGGGAAAGGAATTCCTCAGTCACTTCGTGCTTTTGCTCGTGTTCTATGTTGCGCCTCTCCTTAAGAACTTAGTGACCTGGCCAAAGAAGTTACAGAACATGATGGTTGATTGGCTCGACGTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

208

Amino Acids

24.06

Weight (kDa)

8.45

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 375, 618
AcoI YGGCCR 1 cut(s) 575
AcsI RAATTY 3 cut(s) 101, 287, 506
AcyI GRCGYC 2 cut(s) 372, 615
AdeI CACNNNGTG 1 cut(s) 523
AfaI GTAC 1 cut(s) 270
AfiI CCNNNNNNNGG 3 cut(s) 309, 310, 349
AflII CTTAAG 1 cut(s) 557
AgsI TTSAA 3 cut(s) 294, 403, 453
AjnI CCWGG 3 cut(s) 256, 348, 572
AluBI AGCT 3 cut(s) 34, 40, 54
AluI AGCT 3 cut(s) 34, 40, 54
AlwNI CAGNNNCTG 1 cut(s) 271
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 1 cut(s) 575
ApoI RAATTY 3 cut(s) 101, 287, 506
AseI ATTAAT 1 cut(s) 75
Asp700I GAANNNNTTC 1 cut(s) 289
AspLEI GCGC 1 cut(s) 550
AsuHPI GGTGA 3 cut(s) 148, 272, 356
AvaI CYCGRG 1 cut(s) 198
BalI TGGCCA 1 cut(s) 577
BauI CACGAG 1 cut(s) 533
BccI CCATC 1 cut(s) 592
BceAI ACGGC 1 cut(s) 135
BciT130I CCWGG 3 cut(s) 258, 350, 574
BfrI CTTAAG 1 cut(s) 557
BglII AGATCT 1 cut(s) 83
Bme1390I CCNGG 3 cut(s) 258, 350, 574
BmeT110I CYCGRG 1 cut(s) 198
BmrFI CCNGG 3 cut(s) 258, 350, 574
BpmI CTGGAG 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 110
BsaAI YACGTR 1 cut(s) 178
BsaHI GRCGYC 2 cut(s) 372, 615
Bsc4I CCNNNNNNNGG 3 cut(s) 309, 310, 349
Bse3DI GCAATG 2 cut(s) 4, 234
BseBI CCWGG 3 cut(s) 258, 350, 574
BseGI GGATG 1 cut(s) 339
BseLI CCNNNNNNNGG 3 cut(s) 309, 310, 349
BseMI GCAATG 2 cut(s) 4, 234
BseMII CTCAG 1 cut(s) 526
BshFI GGCC 1 cut(s) 577
BsiHKCI CYCGRG 1 cut(s) 198
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 3 cut(s) 309, 310, 349
BsmFI GGGAC 1 cut(s) 326
BsnI GGCC 1 cut(s) 577
BsoBI CYCGRG 1 cut(s) 198
Bsp143I GATC 2 cut(s) 83, 409
BspANI GGCC 1 cut(s) 577
BspCNI CTCAG 1 cut(s) 525
BspTI CTTAAG 1 cut(s) 557
BsrDI GCAATG 2 cut(s) 4, 234
BssMI GATC 2 cut(s) 83, 409
BssNI GRCGYC 2 cut(s) 372, 615
BssSI CACGAG 1 cut(s) 533
Bst2BI CACGAG 1 cut(s) 533
Bst2UI CCWGG 3 cut(s) 258, 350, 574
Bst6I CTCTTC 2 cut(s) 398, 454
BstACI GRCGYC 2 cut(s) 372, 615
BstAFI CTTAAG 1 cut(s) 557
BstBAI YACGTR 1 cut(s) 178
BstDEI CTNAG 2 cut(s) 512, 565
BstF5I GGATG 1 cut(s) 339
BstHHI GCGC 1 cut(s) 550
BstKTI GATC 2 cut(s) 86, 412
BstMBI GATC 2 cut(s) 83, 409
BstNI CCWGG 3 cut(s) 258, 350, 574
BstSCI CCNGG 3 cut(s) 256, 348, 572
BstX2I RGATCY 1 cut(s) 83
BstYI RGATCY 1 cut(s) 83
BsuRI GGCC 1 cut(s) 577
BtsCI GGATG 1 cut(s) 339
CaiI CAGNNNCTG 1 cut(s) 271
CfoI GCGC 1 cut(s) 550
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 1 cut(s) 269
CviAII CATG 4 cut(s) 172, 204, 368, 595
CviJI RGCY 6 cut(s) 34, 40, 54, 122, 577, 610
CviKI_1 RGCY 6 cut(s) 34, 40, 54, 122, 577, 610
CviQI GTAC 1 cut(s) 269
DdeI CTNAG 2 cut(s) 512, 565
DpnI GATC 2 cut(s) 85, 411
DpnII GATC 2 cut(s) 83, 409
DraIII CACNNNGTG 1 cut(s) 523
EaeI YGGCCR 1 cut(s) 575
Eam1104I CTCTTC 2 cut(s) 398, 454
EarI CTCTTC 2 cut(s) 398, 454
Eco88I CYCGRG 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 3 cut(s) 256, 348, 572
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 4 cut(s) 175, 207, 371, 598
FaqI GGGAC 1 cut(s) 326
FatI CATG 4 cut(s) 171, 203, 367, 594
FokI GGATG 1 cut(s) 326
GlaI GCGC 1 cut(s) 549
GsuI CTGGAG 1 cut(s) 56
HaeIII GGCC 1 cut(s) 577
HhaI GCGC 1 cut(s) 550
Hin1I GRCGYC 2 cut(s) 372, 615
Hin1II CATG 4 cut(s) 175, 207, 371, 598
Hin6I GCGC 1 cut(s) 548
HinP1I GCGC 1 cut(s) 548
HinfI GANTC 3 cut(s) 43, 446, 458
HphI GGTGA 3 cut(s) 148, 272, 356
Hpy166II GTNNAC 2 cut(s) 263, 324
Hpy188III TCNNGA 4 cut(s) 65, 127, 198, 311
Hpy8I GTNNAC 2 cut(s) 263, 324
Hpy99I CGWCG 1 cut(s) 617
HpyAV CCTTC 1 cut(s) 211
HpyCH4IV ACGT 3 cut(s) 177, 372, 615
HpyCH4V TGCA 3 cut(s) 4, 9, 175
HpyF3I CTNAG 2 cut(s) 512, 565
HpySE526I ACGT 3 cut(s) 177, 372, 615
Hsp92I GRCGYC 2 cut(s) 372, 615
Hsp92II CATG 4 cut(s) 175, 207, 371, 598
HspAI GCGC 1 cut(s) 548
Kzo9I GATC 2 cut(s) 83, 409
LmnI GCTCC 2 cut(s) 37, 259
MabI ACCWGGT 1 cut(s) 348
MaeII ACGT 3 cut(s) 177, 372, 615
MaeIII GTNAC 4 cut(s) 23, 515, 568, 585
MalI GATC 2 cut(s) 85, 411
MboI GATC 2 cut(s) 83, 409
MboII GAAGA 2 cut(s) 415, 441
MflI RGATCY 1 cut(s) 83
MlsI TGGCCA 1 cut(s) 577
MluCI AATT 6 cut(s) 72, 101, 111, 247, 287, 506
MluNI TGGCCA 1 cut(s) 577
MlyI GAGTC 1 cut(s) 440
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 3 cut(s) 372, 521, 561
Mox20I TGGCCA 1 cut(s) 577
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 289
MscI TGGCCA 1 cut(s) 577
MseI TTAA 3 cut(s) 75, 558, 622
MslI CAYNNNNRTG 2 cut(s) 180, 249
Msp20I TGGCCA 1 cut(s) 577
MspCI CTTAAG 1 cut(s) 557
MspR9I CCNGG 3 cut(s) 258, 350, 574
MvaI CCWGG 3 cut(s) 258, 350, 574
NdeII GATC 2 cut(s) 83, 409
NlaIII CATG 4 cut(s) 175, 207, 371, 598
NmuCI GTSAC 3 cut(s) 23, 515, 568
NsiI ATGCAT 1 cut(s) 6
PaeR7I CTCGAG 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 2 cut(s) 43, 458
PflFI GACNNNGTC 1 cut(s) 350
PleI GAGTC 1 cut(s) 440
PpsI GAGTC 1 cut(s) 440
Ppu21I YACGTR 1 cut(s) 178
PshBI ATTAAT 1 cut(s) 75
Psp6I CCWGG 3 cut(s) 256, 348, 572
PspGI CCWGG 3 cut(s) 256, 348, 572
PstNI CAGNNNCTG 1 cut(s) 271
PsuI RGATCY 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 350
RsaI GTAC 1 cut(s) 270
RsaNI GTAC 1 cut(s) 269
RseI CAYNNNNRTG 2 cut(s) 180, 249
SaqAI TTAA 3 cut(s) 75, 558, 622
Sau3AI GATC 2 cut(s) 83, 409
SchI GAGTC 1 cut(s) 440
ScrFI CCNGG 3 cut(s) 258, 350, 574
SexAI ACCWGGT 1 cut(s) 348
Sfr274I CTCGAG 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 2 cut(s) 180, 249
SmlI CTYRAG 3 cut(s) 125, 198, 557
SmoI CTYRAG 3 cut(s) 125, 198, 557
Sse9I AATT 6 cut(s) 72, 101, 111, 247, 287, 506
StyD4I CCNGG 3 cut(s) 256, 348, 572
TaiI ACGT 3 cut(s) 180, 375, 618
TaqI TCGA 2 cut(s) 199, 612
TasI AATT 6 cut(s) 72, 101, 111, 247, 287, 506
TfiI GAWTC 2 cut(s) 43, 458
Tru1I TTAA 3 cut(s) 75, 558, 622
Tru9I TTAA 3 cut(s) 75, 558, 622
TseFI GTSAC 3 cut(s) 23, 515, 568
Tsp45I GTSAC 3 cut(s) 23, 515, 568
TspDTI ATGAA 2 cut(s) 160, 321
TspGWI ACGGA 1 cut(s) 297
Tth111I GACNNNGTC 1 cut(s) 350
Vha464I CTTAAG 1 cut(s) 557
VspI ATTAAT 1 cut(s) 75
XapI RAATTY 3 cut(s) 101, 287, 506
XhoI CTCGAG 1 cut(s) 198
XmnI GAANNNNTTC 1 cut(s) 289
ZraI GACGTC 2 cut(s) 373, 616
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.