pycom01g00310

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000846
Physical Location & Seq
Forward (+)
429150 .. 431626
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00310.1

Sequence Viewer

Length: 570 bp
ATGGCTCTCAAGAACTTCTCTGAAATGTTCAAAAGTATCACCTATAAGGATTTCATGCGCGCTTATGGTTTAGTTACTTCTCGAGCATGGGGAAGCACGAAGGGTTATTCCCTGATCCCATTTGCTGATTTTTTGAATCATGACGGGACTTCGGAATCAATTGTGTTGAGTGATGACGACAAACAGTTCTCAGAGGTACTGATAGGATACGGAAAATTTTCAAATGCTACCCTGCTGCTGGACTTTGGGTTTACACATTCATACAACATTCACGACCAGGTCCAGATCCAGGGCAATATACCTCATCATGATGTACTTCGTGAAATGAAGTGGGAACTTTTGAAGAGACATCACAGACCAATCAGTAATGATGTCAATTGTTTCGACTCTTTAGTGGATTCTTTCGCTATCGTAGTGATGCACTGTATCTCTTTTATTTCCTTTTTTGTTTTTCCCCTCATTTTGTTCCTTTGCCTACGTGATGGTCCTTGTCCCCCGAATGTAGTGGTAATATGTGAAGTCACCAAATATTCTGCTTCTTTTGTTCCATATCCAGTCTTGTTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00310 map00310
Pfam Domains
Protein Families
Rubis-subs-bind SET

Protein Analysis

190

Amino Acids

21.61

Weight (kDa)

6.02

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 60
AclWI GGATC 2 cut(s) 109, 280
AcsI RAATTY 1 cut(s) 215
AfaI GTAC 2 cut(s) 198, 315
AfiI CCNNNNNNNGG 2 cut(s) 238, 289
AgsI TTSAA 4 cut(s) 31, 136, 222, 343
AjnI CCWGG 2 cut(s) 276, 288
Alw26I GTCTC 1 cut(s) 340
AlwI GGATC 2 cut(s) 109, 280
Ama87I CYCGRG 1 cut(s) 81
ApeKI GCWGC 1 cut(s) 235
ApoI RAATTY 1 cut(s) 215
Asp700I GAANNNNTTC 1 cut(s) 217
AspLEI GCGC 2 cut(s) 60, 62
AspS9I GGNCC 2 cut(s) 280, 485
AsuHPI GGTGA 2 cut(s) 31, 514
AvaI CYCGRG 1 cut(s) 81
AvaII GGWCC 2 cut(s) 280, 485
BbvI GCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 476
BciT130I CCWGG 2 cut(s) 278, 290
BciVI GTATCC 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 340
BfuI GTATCC 1 cut(s) 200
BisI GCNGC 1 cut(s) 236
BlsI GCNGC 1 cut(s) 237
Bme1390I CCNGG 2 cut(s) 278, 290
Bme18I GGWCC 2 cut(s) 280, 485
BmeT110I CYCGRG 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 280, 485
BmrFI CCNGG 2 cut(s) 278, 290
BmsI GCATC 1 cut(s) 408
BsaAI YACGTR 1 cut(s) 479
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 289
Bse1I ACTGG 1 cut(s) 554
BseBI CCWGG 2 cut(s) 278, 290
BseDI CCNNGG 1 cut(s) 289
BseLI CCNNNNNNNGG 2 cut(s) 238, 289
BseMII CTCAG 1 cut(s) 204
BseNI ACTGG 1 cut(s) 554
BsePI GCGCGC 1 cut(s) 58
BseXI GCAGC 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 81
BslFI GGGAC 2 cut(s) 160, 477
BslI CCNNNNNNNGG 2 cut(s) 238, 289
BsmAI GTCTC 1 cut(s) 340
BsmFI GGGAC 2 cut(s) 160, 477
BsoBI CYCGRG 1 cut(s) 81
Bsp143I GATC 2 cut(s) 114, 285
BspCNI CTCAG 1 cut(s) 203
BspFNI CGCG 1 cut(s) 60
BspHI TCATGA 2 cut(s) 139, 307
BspPI GGATC 2 cut(s) 109, 280
BsrI ACTGG 1 cut(s) 554
BssECI CCNNGG 1 cut(s) 289
BssHII GCGCGC 1 cut(s) 58
BssMI GATC 2 cut(s) 114, 285
Bst2UI CCWGG 2 cut(s) 278, 290
Bst4CI ACNGT 2 cut(s) 186, 425
Bst6I CTCTTC 1 cut(s) 338
BstBAI YACGTR 1 cut(s) 479
BstC8I GCNNGC 1 cut(s) 60
BstDEI CTNAG 1 cut(s) 190
BstFNI CGCG 1 cut(s) 60
BstHHI GCGC 2 cut(s) 60, 62
BstKTI GATC 2 cut(s) 117, 288
BstMAI GTCTC 1 cut(s) 340
BstMBI GATC 2 cut(s) 114, 285
BstNI CCWGG 2 cut(s) 278, 290
BstSCI CCNGG 2 cut(s) 276, 288
BstUI CGCG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 222
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuI GTATCC 1 cut(s) 200
BtsIMutI CAGTG 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 60
CciI TCATGA 2 cut(s) 139, 307
CfoI GCGC 2 cut(s) 60, 62
Cfr13I GGNCC 2 cut(s) 280, 485
CsiI ACCWGGT 1 cut(s) 276
Csp6I GTAC 2 cut(s) 197, 314
CviAII CATG 4 cut(s) 55, 87, 140, 308
CviJI RGCY 1 cut(s) 5
CviKI_1 RGCY 1 cut(s) 5
CviQI GTAC 2 cut(s) 197, 314
DdeI CTNAG 1 cut(s) 190
DpnI GATC 2 cut(s) 116, 287
DpnII GATC 2 cut(s) 114, 285
Eam1104I CTCTTC 1 cut(s) 338
EarI CTCTTC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 280, 485
Eco88I CYCGRG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 276, 288
FaeI CATG 4 cut(s) 58, 90, 143, 311
FaqI GGGAC 2 cut(s) 160, 477
FatI CATG 4 cut(s) 54, 86, 139, 307
Fnu4HI GCNGC 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 236
GlaI GCGC 2 cut(s) 59, 61
GluI GCNGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 60, 62
Hin1II CATG 4 cut(s) 58, 90, 143, 311
Hin6I GCGC 2 cut(s) 58, 60
HinP1I GCGC 2 cut(s) 58, 60
HinfI GANTC 4 cut(s) 136, 155, 386, 398
HphI GGTGA 2 cut(s) 31, 514
Hpy166II GTNNAC 1 cut(s) 252
Hpy188I TCNGA 3 cut(s) 22, 154, 193
Hpy188III TCNNGA 7 cut(s) 10, 81, 140, 272, 283, 308, 320
Hpy8I GTNNAC 1 cut(s) 252
HpyAV CCTTC 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 186, 425
HpyCH4IV ACGT 1 cut(s) 478
HpyCH4V TGCA 1 cut(s) 421
HpyF3I CTNAG 1 cut(s) 190
HpySE526I ACGT 1 cut(s) 478
Hsp92II CATG 4 cut(s) 58, 90, 143, 311
HspAI GCGC 2 cut(s) 58, 60
Kzo9I GATC 2 cut(s) 114, 285
LpnPI CCDG 8 cut(s) 125, 224, 245, 263, 275, 290, 296, 302
Lsp1109I GCAGC 1 cut(s) 222
LweI GCATC 1 cut(s) 408
MabI ACCWGGT 1 cut(s) 276
MaeII ACGT 1 cut(s) 478
MaeIII GTNAC 2 cut(s) 73, 520
MalI GATC 2 cut(s) 116, 287
MboI GATC 2 cut(s) 114, 285
MboII GAAGA 1 cut(s) 355
MfeI CAATTG 2 cut(s) 159, 376
MflI RGATCY 1 cut(s) 285
MluCI AATT 4 cut(s) 159, 215, 376, 565
MlyI GAGTC 1 cut(s) 380
MnlI CCTC 3 cut(s) 187, 312, 467
MroXI GAANNNNTTC 1 cut(s) 217
MseI TTAA 2 cut(s) 564, 568
MslI CAYNNNNRTG 1 cut(s) 309
MspR9I CCNGG 2 cut(s) 278, 290
MunI CAATTG 2 cut(s) 159, 376
MvaI CCWGG 2 cut(s) 278, 290
MvnI CGCG 1 cut(s) 60
NdeII GATC 2 cut(s) 114, 285
NlaIII CATG 4 cut(s) 58, 90, 143, 311
NmuCI GTSAC 1 cut(s) 520
PacI TTAATTAA 1 cut(s) 568
PaeR7I CTCGAG 1 cut(s) 81
PagI TCATGA 2 cut(s) 139, 307
PauI GCGCGC 1 cut(s) 58
PdmI GAANNNNTTC 1 cut(s) 217
PfeI GAWTC 3 cut(s) 136, 155, 398
PflFI GACNNNGTC 1 cut(s) 278
PkrI GCNGC 1 cut(s) 237
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
Ppu21I YACGTR 1 cut(s) 479
Psp6I CCWGG 2 cut(s) 276, 288
PspGI CCWGG 2 cut(s) 276, 288
PspPI GGNCC 2 cut(s) 280, 485
PsuI RGATCY 1 cut(s) 285
PsyI GACNNNGTC 1 cut(s) 278
PteI GCGCGC 1 cut(s) 58
RsaI GTAC 2 cut(s) 198, 315
RsaNI GTAC 2 cut(s) 197, 314
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 2 cut(s) 564, 568
SatI GCNGC 1 cut(s) 236
Sau3AI GATC 2 cut(s) 114, 285
Sau96I GGNCC 2 cut(s) 280, 485
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 2 cut(s) 278, 290
SetI ASST 5 cut(s) 44, 198, 282, 304, 481
SexAI ACCWGGT 1 cut(s) 276
SfaNI GCATC 1 cut(s) 408
Sfr274I CTCGAG 1 cut(s) 81
SinI GGWCC 2 cut(s) 280, 485
SlaI CTCGAG 1 cut(s) 81
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 2 cut(s) 8, 81
SmoI CTYRAG 2 cut(s) 8, 81
Sse9I AATT 4 cut(s) 159, 215, 376, 565
SspI AATATT 1 cut(s) 530
StyD4I CCNGG 2 cut(s) 276, 288
TaaI ACNGT 2 cut(s) 186, 425
TaiI ACGT 1 cut(s) 481
TaqI TCGA 2 cut(s) 82, 384
TasI AATT 4 cut(s) 159, 215, 376, 565
TatI WGTACW 1 cut(s) 313
TfiI GAWTC 3 cut(s) 136, 155, 398
Tru1I TTAA 2 cut(s) 564, 568
Tru9I TTAA 2 cut(s) 564, 568
TscAI CASTG 1 cut(s) 428
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 3 cut(s) 43, 249, 341
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 1 cut(s) 428
Tth111I GACNNNGTC 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 280, 485
XapI RAATTY 1 cut(s) 215
XhoI CTCGAG 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.