AT3G58130

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001436
Physical Location & Seq
Forward (+)
21527530 .. 21529905
2376 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G58130.1

Sequence Viewer

Length: 774 bp
ATGGCATGGCTTGTGGTTTCTGTTTCTCTGATTGTTATATGGTTGGCTTCTTTCTGCAAAATATTCTTCCGAGCAACATCTATCTCCGGAGCTGCTATTCTTGATGATGGTAAAACCCCTCAGAAGAAAAATGTACTGTTTGTTATCGCACATCCTGATGATGAATCGATGTTCTTTTCTCCAACAATAAACTACTTAGCTTCCAATGCTTGCAATCTTCACATGTTATGCTTGTCCACTGGTAACGCTGATGGTATGGGAAGCATTAGGAACAATGAGCTGCATCGGGCATGTGCGGTGCTCAAGGTTCCGCTTCAACAGTTAAAGATTCTGAACCATCCTAATTTGCAGGATGGTTTTGGCCAATTATGGAGCCACGATTTGCTAACAGAGATCATCGAAGAAGAAGTCACTAAACACGATATCCACACAATAATAACATTCGATAACTATGGTGTTTCGGGTCATTGCAATCACCGAGATGTGCATCGTGGAGTATTAAAGTTCTTGCAGACTAATTCAGGAAGAAATGTCAAAGCTTGGGAACTCGTAAGCCTTAATATCTTTCGCAAGTACTGTGGACCTGTCGACATTTGGCTGTCAATTTTATCCGCCAAAATACATCCGAGCAAGGTAATCATCATAAACGAGCAGCCTTGGAAAAGCTTCAAAGCAATGGCACAACATTTAAGCCAGTGGGTTTGGTTTCGAAAGCTTTTTGTTTCGTTTTCAAGCTATACATATGCGAATACACTTAGTAGAATCAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-L

Protein Analysis

257

Amino Acids

29.16

Weight (kDa)

8.88

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-L PF02585 46 - 168 3.5e-24 GlcNAc-PI de-N-acetylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 588
AccIII TCCGGA 1 cut(s) 86
AciI CCGC 3 cut(s) 296, 311, 612
AcoI YGGCCR 1 cut(s) 361
AfaI GTAC 2 cut(s) 135, 575
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 3 cut(s) 317, 670, 732
AluBI AGCT 7 cut(s) 92, 200, 280, 539, 666, 715, 735
AluI AGCT 7 cut(s) 92, 200, 280, 539, 666, 715, 735
Alw21I GWGCWC 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 86
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 3 cut(s) 92, 280, 652
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
Asp700I GAANNNNTTC 1 cut(s) 665
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 1 cut(s) 467
AsuII TTCGAA 1 cut(s) 709
AvaII GGWCC 1 cut(s) 581
BalI TGGCCA 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 79, 267, 664
BccI CCATC 4 cut(s) 101, 245, 345, 347
BisI GCNGC 3 cut(s) 93, 281, 653
BlsI GCNGC 3 cut(s) 94, 282, 654
BmcAI AGTACT 1 cut(s) 575
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 2 cut(s) 309, 374
BmsI GCATC 2 cut(s) 292, 496
Bpu14I TTCGAA 1 cut(s) 709
BpuEI CTTGAG 1 cut(s) 287
Bsa29I ATCGAT 1 cut(s) 167
BsaBI GATNNNNATC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 656
BsaWI WCCGGW 1 cut(s) 86
Bse1I ACTGG 2 cut(s) 244, 694
Bse3DI GCAATG 2 cut(s) 466, 681
Bse8I GATNNNNATC 1 cut(s) 486
BseAI TCCGGA 1 cut(s) 86
BseCI ATCGAT 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 656
BseGI GGATG 4 cut(s) 151, 337, 358, 622
BseJI GATNNNNATC 1 cut(s) 486
BseMI GCAATG 2 cut(s) 466, 681
BseMII CTCAG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 244, 694
BseXI GCAGC 3 cut(s) 79, 267, 664
BshFI GGCC 1 cut(s) 363
BshVI ATCGAT 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 1 cut(s) 87
BsnI GGCC 1 cut(s) 363
Bsp119I TTCGAA 1 cut(s) 709
Bsp1286I GDGCHC 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 86
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 3 cut(s) 296, 311, 612
BspANI GGCC 1 cut(s) 363
BspCNI CTCAG 1 cut(s) 133
BspDI ATCGAT 1 cut(s) 167
BspEI TCCGGA 1 cut(s) 86
BspLI GGNNCC 2 cut(s) 309, 374
BspT104I TTCGAA 1 cut(s) 709
BsrDI GCAATG 2 cut(s) 466, 681
BsrI ACTGG 2 cut(s) 244, 694
BssECI CCNNGG 1 cut(s) 656
BssMI GATC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 656
Bst4CI ACNGT 3 cut(s) 138, 321, 578
BstBI TTCGAA 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 211
BstDEI CTNAG 3 cut(s) 120, 196, 755
BstF5I GGATG 4 cut(s) 151, 337, 358, 622
BstKTI GATC 1 cut(s) 396
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 1 cut(s) 206
BstNSI RCATGY 2 cut(s) 226, 294
BstV1I GCAGC 3 cut(s) 79, 267, 664
Bsu15I ATCGAT 1 cut(s) 167
BsuRI GGCC 1 cut(s) 363
BsuTUI ATCGAT 1 cut(s) 167
BtsCI GGATG 4 cut(s) 151, 337, 358, 622
BtsIMutI CAGTG 2 cut(s) 237, 701
Cac8I GCNNGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 581
ClaI ATCGAT 1 cut(s) 167
Csp6I GTAC 2 cut(s) 134, 574
CspCI CAANNNNNGTGG 2 cut(s) 559, 594
CviAII CATG 3 cut(s) 6, 223, 291
CviQI GTAC 2 cut(s) 134, 574
DdeI CTNAG 3 cut(s) 120, 196, 755
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 361
EciI GGCGGA 1 cut(s) 601
Eco130I CCWWGG 1 cut(s) 656
Eco32I GATATC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 581
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 656
ErhI CCWWGG 1 cut(s) 656
FaeI CATG 3 cut(s) 9, 226, 294
FatI CATG 3 cut(s) 5, 222, 290
FauNDI CATATG 1 cut(s) 742
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 3 cut(s) 93, 281, 653
FokI GGATG 4 cut(s) 138, 324, 365, 609
Fsp4HI GCNGC 3 cut(s) 93, 281, 653
GluI GCNGC 3 cut(s) 93, 281, 653
HaeIII GGCC 1 cut(s) 363
HapII CCGG 1 cut(s) 87
Hin1II CATG 3 cut(s) 9, 226, 294
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 3 cut(s) 537, 664, 713
HinfI GANTC 3 cut(s) 164, 328, 762
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 3 cut(s) 237, 581, 589
Hpy188I TCNGA 5 cut(s) 30, 71, 123, 333, 627
Hpy188III TCNNGA 4 cut(s) 87, 101, 155, 522
Hpy8I GTNNAC 3 cut(s) 237, 581, 589
HpyCH4III ACNGT 3 cut(s) 138, 321, 578
HpyCH4V TGCA 7 cut(s) 57, 213, 283, 349, 471, 487, 511
HpyF10VI GCNNNNNNNGC 1 cut(s) 206
HpyF3I CTNAG 3 cut(s) 120, 196, 755
Hsp92II CATG 3 cut(s) 9, 226, 294
Kpn2I TCCGGA 1 cut(s) 86
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 2 cut(s) 89, 372
LpnPI CCDG 7 cut(s) 100, 168, 225, 335, 507, 597, 707
Lsp1109I GCAGC 3 cut(s) 79, 267, 664
LweI GCATC 2 cut(s) 292, 496
MaeIII GTNAC 2 cut(s) 242, 409
MalI GATC 1 cut(s) 395
MboI GATC 1 cut(s) 393
MboII GAAGA 6 cut(s) 58, 136, 209, 413, 416, 537
MhlI GDGCHC 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 363
MluCI AATT 4 cut(s) 343, 365, 517, 603
MluNI TGGCCA 1 cut(s) 363
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 1 cut(s) 129
Mox20I TGGCCA 1 cut(s) 363
MroI TCCGGA 1 cut(s) 86
MroXI GAANNNNTTC 1 cut(s) 665
MscI TGGCCA 1 cut(s) 363
MseI TTAA 4 cut(s) 323, 500, 558, 689
MslI CAYNNNNRTG 2 cut(s) 156, 480
Msp20I TGGCCA 1 cut(s) 363
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 1 cut(s) 206
NdeI CATATG 1 cut(s) 742
NdeII GATC 1 cut(s) 393
NlaIII CATG 3 cut(s) 9, 226, 294
NlaIV GGNNCC 2 cut(s) 309, 374
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 2 cut(s) 226, 294
NspV TTCGAA 1 cut(s) 709
PciI ACATGT 1 cut(s) 222
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 3 cut(s) 164, 328, 762
PkrI GCNGC 3 cut(s) 94, 282, 654
PscI ACATGT 1 cut(s) 222
PspN4I GGNNCC 2 cut(s) 309, 374
PspPI GGNCC 1 cut(s) 581
RsaI GTAC 2 cut(s) 135, 575
RsaNI GTAC 2 cut(s) 134, 574
RseI CAYNNNNRTG 2 cut(s) 156, 480
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 4 cut(s) 323, 500, 558, 689
SatI GCNGC 3 cut(s) 93, 281, 653
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 1 cut(s) 581
ScaI AGTACT 1 cut(s) 575
SduI GDGCHC 1 cut(s) 303
SfaNI GCATC 2 cut(s) 292, 496
SfuI TTCGAA 1 cut(s) 709
SinI GGWCC 1 cut(s) 581
SmiMI CAYNNNNRTG 2 cut(s) 156, 480
SmlI CTYRAG 1 cut(s) 302
SmoI CTYRAG 1 cut(s) 302
Sse9I AATT 4 cut(s) 343, 365, 517, 603
SsiI CCGC 3 cut(s) 296, 311, 612
SspI AATATT 1 cut(s) 63
StyI CCWWGG 1 cut(s) 656
TaaI ACNGT 3 cut(s) 138, 321, 578
TaqI TCGA 5 cut(s) 167, 399, 444, 588, 709
TasI AATT 4 cut(s) 343, 365, 517, 603
TatI WGTACW 2 cut(s) 133, 573
TfiI GAWTC 3 cut(s) 164, 328, 762
Tru1I TTAA 4 cut(s) 323, 500, 558, 689
Tru9I TTAA 4 cut(s) 323, 500, 558, 689
TscAI CASTG 2 cut(s) 244, 701
TseFI GTSAC 1 cut(s) 409
TseI GCWGC 3 cut(s) 92, 280, 652
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 177
TspRI CASTG 2 cut(s) 244, 701
VpaK11BI GGWCC 1 cut(s) 581
XceI RCATGY 2 cut(s) 226, 294
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 665
ZrmI AGTACT 1 cut(s) 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.