MD16G1270300.v1.1

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001436
Physical Location & Seq
Forward (+)
35041278 .. 35046766
5489 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1270300.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGTGGGTGGCAGTGTCAACAAGAGAAATGTTTTGTTCGTTGTTGCCCACCCAGATGACGAGTCCATGTTCTTTACTCCAACAATAAACTATCTGACCGTGAGAGGGCATAATGTTCACATTTTATGCTTGTCAATTGGTGATGCAGATGGCAAAGGAATTACAAGAAAAGAAGAGCTCTACCAGGCTTCTGCAATTCTCAAGGTTCCGCATCAACAAGTGTCGGTTCTGGATCATCCAAATCTTCAGGATGGTTTTGGCAAAGTTTGGAACGATAGCATATTGGCAAATATTATCGAAGAGGAAATTACAAGAAACAACATTGACTTGATAATTACTTTTGATAACTACGGAGTTTCGGGCCATTGTAACCACCGTGATGTGCATTATGGGGTAAGGAAACTCTTACAAGCGTCGTCACAAAGGAAAATTGAAGCCTGGGAACTCGTTAGTACAAACATATTTCGCAAGTATAGCGGACCAGTCGATATCTGGCTGTCCAGTTTAAATCCCATGCAATCCTCACATGAAGTGGTGCATTGCTTGCTCAATGAGCAGCCCAGAAAGAGCTTCCTGGCGATGGCACAGCATTCCAGCCAATGGGTTTGGTTCCGCAAGCTGTTTGTAGCATTTTCCAGTTACACGTATGTGAACACGCTTAAAAAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-L

Protein Analysis

224

Amino Acids

25.46

Weight (kDa)

7.81

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-L PF02585 12 - 134 6.8e-23 GlcNAc-PI de-N-acetylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 600
AciI CCGC 3 cut(s) 209, 477, 613
AclWI GGATC 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 3 cut(s) 105, 580, 600
AflIII ACRYGT 1 cut(s) 642
AgsI TTSAA 1 cut(s) 434
AjnI CCWGG 3 cut(s) 183, 437, 573
AleI CACNNNNGTG 1 cut(s) 647
AluBI AGCT 3 cut(s) 178, 570, 619
AluI AGCT 3 cut(s) 178, 570, 619
Alw21I GWGCWC 1 cut(s) 180
AlwI GGATC 1 cut(s) 240
AoxI GGCC 1 cut(s) 361
ApeKI GCWGC 1 cut(s) 556
AspS9I GGNCC 2 cut(s) 361, 479
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 1 cut(s) 568
BccI CCATC 3 cut(s) 143, 245, 574
BciT130I CCWGG 3 cut(s) 185, 439, 575
BisI GCNGC 1 cut(s) 557
BlsI GCNGC 1 cut(s) 558
Bme1390I CCNGG 3 cut(s) 185, 439, 575
Bme18I GGWCC 1 cut(s) 479
BmgT120I GGNCC 2 cut(s) 361, 479
BmiI GGNNCC 2 cut(s) 207, 611
BmrFI CCNGG 3 cut(s) 185, 439, 575
BmsI GCATC 2 cut(s) 133, 220
BpuEI CTTGAG 1 cut(s) 185
BsaAI YACGTR 1 cut(s) 645
BsaJI CCNNGG 1 cut(s) 438
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 580, 600
Bse1I ACTGG 3 cut(s) 482, 501, 636
Bse3DI GCAATG 1 cut(s) 538
BseBI CCWGG 3 cut(s) 185, 439, 575
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 2 cut(s) 235, 256
BseLI CCNNNNNNNGG 3 cut(s) 105, 580, 600
BseMI GCAATG 1 cut(s) 538
BseNI ACTGG 3 cut(s) 482, 501, 636
BseXI GCAGC 1 cut(s) 568
BshFI GGCC 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 105, 580, 600
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 1 cut(s) 232
BspACI CCGC 3 cut(s) 209, 477, 613
BspANI GGCC 1 cut(s) 363
BspLI GGNNCC 2 cut(s) 207, 611
BspPI GGATC 1 cut(s) 240
BspQI GCTCTTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 538
BsrI ACTGG 3 cut(s) 482, 501, 636
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 1 cut(s) 232
Bst2UI CCWGG 3 cut(s) 185, 439, 575
Bst4CI ACNGT 2 cut(s) 100, 377
Bst6I CTCTTC 2 cut(s) 168, 294
BstAPI GCANNNNNTGC 1 cut(s) 544
BstBAI YACGTR 1 cut(s) 645
BstC8I GCNNGC 2 cut(s) 545, 617
BstF5I GGATG 2 cut(s) 235, 256
BstKTI GATC 1 cut(s) 235
BstMBI GATC 1 cut(s) 232
BstMWI GCNNNNNNNGC 3 cut(s) 474, 544, 553
BstNI CCWGG 3 cut(s) 185, 439, 575
BstSCI CCNGG 3 cut(s) 183, 437, 573
BstV1I GCAGC 1 cut(s) 568
BsuRI GGCC 1 cut(s) 363
BtgZI GCGATG 1 cut(s) 593
BtsCI GGATG 2 cut(s) 235, 256
BtsI GCAGTG 1 cut(s) 19
BtsIMutI CAGTG 1 cut(s) 19
Cac8I GCNNGC 2 cut(s) 545, 617
Cfr13I GGNCC 2 cut(s) 361, 479
CseI GACGC 1 cut(s) 402
Csp6I GTAC 1 cut(s) 453
CviAII CATG 3 cut(s) 67, 514, 527
CviJI RGCY 9 cut(s) 178, 188, 363, 437, 496, 559, 570, 597, 619
CviKI_1 RGCY 9 cut(s) 178, 188, 363, 437, 496, 559, 570, 597, 619
CviQI GTAC 1 cut(s) 453
DpnI GATC 1 cut(s) 234
DpnII GATC 1 cut(s) 232
DraI TTTAAA 1 cut(s) 507
Eam1104I CTCTTC 2 cut(s) 168, 294
EarI CTCTTC 2 cut(s) 168, 294
Ecl136II GAGCTC 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 180
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 479
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 178
EcoRII CCWGG 3 cut(s) 183, 437, 573
EcoRV GATATC 1 cut(s) 490
EcoT38I GRGCYC 1 cut(s) 180
FaeI CATG 3 cut(s) 70, 517, 530
FatI CATG 3 cut(s) 66, 513, 526
Fnu4HI GCNGC 1 cut(s) 557
FokI GGATG 2 cut(s) 222, 263
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 1 cut(s) 557
GluI GCNGC 1 cut(s) 557
HaeIII GGCC 1 cut(s) 363
HgaI GACGC 1 cut(s) 402
Hin1II CATG 3 cut(s) 70, 517, 530
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 1 cut(s) 62
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 3 cut(s) 19, 118, 652
Hpy188I TCNGA 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 230, 248
Hpy8I GTNNAC 3 cut(s) 19, 118, 652
Hpy99I CGWCG 1 cut(s) 418
HpyCH4III ACNGT 2 cut(s) 100, 377
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 5 cut(s) 146, 194, 385, 517, 538
HpyF10VI GCNNNNNNNGC 3 cut(s) 474, 544, 553
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 3 cut(s) 70, 517, 530
Kzo9I GATC 1 cut(s) 232
LguI GCTCTTC 1 cut(s) 168
Lsp1109I GCAGC 1 cut(s) 568
LweI GCATC 2 cut(s) 133, 220
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 3 cut(s) 368, 417, 638
MalI GATC 1 cut(s) 234
MboI GATC 1 cut(s) 232
MboII GAAGA 3 cut(s) 185, 236, 311
MfeI CAATTG 1 cut(s) 135
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 6 cut(s) 135, 159, 195, 306, 333, 429
MlyI GAGTC 1 cut(s) 71
MmeI TCCRAC 1 cut(s) 104
MnlI CCTC 3 cut(s) 98, 295, 532
MseI TTAA 2 cut(s) 506, 660
MslI CAYNNNNRTG 3 cut(s) 54, 378, 647
MspR9I CCNGG 3 cut(s) 185, 439, 575
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 3 cut(s) 185, 439, 575
MwoI GCNNNNNNNGC 3 cut(s) 474, 544, 553
NdeII GATC 1 cut(s) 232
NlaIII CATG 3 cut(s) 70, 517, 530
NlaIV GGNNCC 2 cut(s) 207, 611
NmuCI GTSAC 1 cut(s) 417
OliI CACNNNNGTG 1 cut(s) 647
PciSI GCTCTTC 1 cut(s) 168
PctI GAATGC 1 cut(s) 589
PflMI CCANNNNNTGG 1 cut(s) 600
PkrI GCNGC 1 cut(s) 558
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
Ppu21I YACGTR 1 cut(s) 645
Psp124BI GAGCTC 1 cut(s) 180
Psp6I CCWGG 3 cut(s) 183, 437, 573
PspGI CCWGG 3 cut(s) 183, 437, 573
PspN4I GGNNCC 2 cut(s) 207, 611
PspPI GGNCC 2 cut(s) 361, 479
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
RseI CAYNNNNRTG 3 cut(s) 54, 378, 647
SacI GAGCTC 1 cut(s) 180
SapI GCTCTTC 1 cut(s) 168
SaqAI TTAA 2 cut(s) 506, 660
SatI GCNGC 1 cut(s) 557
Sau3AI GATC 1 cut(s) 232
Sau96I GGNCC 2 cut(s) 361, 479
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 185, 439, 575
SduI GDGCHC 1 cut(s) 180
SetI ASST 5 cut(s) 180, 207, 572, 621, 647
SfaNI GCATC 2 cut(s) 133, 220
SinI GGWCC 1 cut(s) 479
SmiMI CAYNNNNRTG 3 cut(s) 54, 378, 647
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
Sse9I AATT 6 cut(s) 135, 159, 195, 306, 333, 429
SsiI CCGC 3 cut(s) 209, 477, 613
SspI AATATT 1 cut(s) 292
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 183, 437, 573
TaaI ACNGT 2 cut(s) 100, 377
TaiI ACGT 1 cut(s) 647
TaqI TCGA 2 cut(s) 297, 486
TasI AATT 6 cut(s) 135, 159, 195, 306, 333, 429
TatI WGTACW 1 cut(s) 452
Tru1I TTAA 2 cut(s) 506, 660
Tru9I TTAA 2 cut(s) 506, 660
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 417
TseI GCWGC 1 cut(s) 556
Tsp45I GTSAC 1 cut(s) 417
TspDTI ATGAA 1 cut(s) 543
TspGWI ACGGA 1 cut(s) 366
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 600
VpaK11BI GGWCC 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.