Rh7CG216500

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001436
Physical Location & Seq
Forward (+)
19690414 .. 19700783
10370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG216500.1

Sequence Viewer

Length: 654 bp
ATGCCCATTGAATTTCGAAAAATAGAGCACTCTGAACATGATGTTACCGACGACTGCGTTGGCCGGAGGAGGAGAAGCTCTGCTTCTAGGAGGCGTCAGGTGCATTTGAGATGGCGTGGTGATGCAGATGGTAAAGGAAATACTAGAAAAGAAGAGCTCTACCAGGCTTCTGCAACCCTCAAGGTTGCGCATCAACATGTGAAAATTCTGGACCATCCAGATCTTCAGGATGGTTTTGGTAAGGTGTGGAATCACAATTTATTGGCAAAGATTATTGAAGAGGAAGTCATAAGTCAGGGCATTGACTTGATAATTACTTTTGATAATTATGGAGTTTCGGGACACTGTAACCATTGTGACGTGCATTACGGAGTAAGGAAGCTTTTACTTGCATTGTCACAAAGGAATGTTGAAGCCTGGGAACTTGTCAGTACTAACATATTCCGCAAGTATAGTGGACCAGTGGATATATGGTTCTCCAATTTATATTCTATGCAATGCTCAAATGAAATGTTGCATTGCTTGGTAAATGAACAACCCAGAAAGAGCTTCCTGGCAATGGCACAACATGCAAGCCAATGGGTTTGGTTTCGCAAGCTTTTTGTGGCATTTTCCAGTTGTACCTATGTGAACACGCTTAGAAAGATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-L

Protein Analysis

217

Amino Acids

25.28

Weight (kDa)

9.04

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-L PF02585 40 - 127 1.1e-07 GlcNAc-PI de-N-acetylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 189
AciI CCGC 1 cut(s) 445
AcoI YGGCCR 1 cut(s) 61
AcsI RAATTY 2 cut(s) 11, 204
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 94
AfaI GTAC 2 cut(s) 433, 622
AfiI CCNNNNNNNGG 1 cut(s) 559
AflIII ACRYGT 1 cut(s) 196
AgsI TTSAA 3 cut(s) 11, 278, 413
AjiI CACGTC 1 cut(s) 361
AjnI CCWGG 3 cut(s) 162, 416, 552
AloI GAACNNNNNNTCC 2 cut(s) 458, 490
AluBI AGCT 5 cut(s) 78, 157, 382, 549, 598
AluI AGCT 5 cut(s) 78, 157, 382, 549, 598
Alw21I GWGCWC 2 cut(s) 30, 159
AoxI GGCC 1 cut(s) 61
ApoI RAATTY 2 cut(s) 11, 204
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 211, 458
AsuHPI GGTGA 1 cut(s) 131
AsuII TTCGAA 1 cut(s) 16
AvaII GGWCC 2 cut(s) 211, 458
BanII GRGCYC 1 cut(s) 159
Bbv12I GWGCWC 2 cut(s) 30, 159
BccI CCATC 4 cut(s) 105, 122, 222, 224
BciT130I CCWGG 3 cut(s) 164, 418, 554
BfaI CTAG 2 cut(s) 87, 144
BglII AGATCT 1 cut(s) 220
BmcAI AGTACT 1 cut(s) 433
Bme1390I CCNGG 3 cut(s) 164, 418, 554
Bme18I GGWCC 2 cut(s) 211, 458
BmgBI CACGTC 1 cut(s) 361
BmgT120I GGNCC 2 cut(s) 211, 458
BmrFI CCNGG 3 cut(s) 164, 418, 554
BmsI GCATC 2 cut(s) 112, 199
Bpu14I TTCGAA 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 94
BsaJI CCNNGG 1 cut(s) 417
Bsc4I CCNNNNNNNGG 1 cut(s) 559
Bse1I ACTGG 2 cut(s) 461, 615
Bse3DI GCAATG 3 cut(s) 503, 517, 564
BseBI CCWGG 3 cut(s) 164, 418, 554
BseDI CCNNGG 1 cut(s) 417
BseGI GGATG 2 cut(s) 214, 235
BseLI CCNNNNNNNGG 1 cut(s) 559
BseMI GCAATG 3 cut(s) 503, 517, 564
BseNI ACTGG 2 cut(s) 461, 615
BseRI GAGGAG 2 cut(s) 82, 85
BshFI GGCC 1 cut(s) 63
BsiHKAI GWGCWC 2 cut(s) 30, 159
BsiSI CCGG 1 cut(s) 64
BslFI GGGAC 1 cut(s) 354
BslI CCNNNNNNNGG 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 354
BsnI GGCC 1 cut(s) 63
Bsp119I TTCGAA 1 cut(s) 16
Bsp1286I GDGCHC 2 cut(s) 30, 159
Bsp143I GATC 1 cut(s) 220
BspACI CCGC 1 cut(s) 445
BspANI GGCC 1 cut(s) 63
BspQI GCTCTTC 1 cut(s) 147
BspT104I TTCGAA 1 cut(s) 16
BsrDI GCAATG 3 cut(s) 503, 517, 564
BsrI ACTGG 2 cut(s) 461, 615
BssECI CCNNGG 1 cut(s) 417
BssMI GATC 1 cut(s) 220
BssNI GRCGYC 1 cut(s) 94
Bst2UI CCWGG 3 cut(s) 164, 418, 554
Bst4CI ACNGT 1 cut(s) 347
Bst6I CTCTTC 2 cut(s) 147, 273
BstACI GRCGYC 1 cut(s) 94
BstAPI GCANNNNNTGC 1 cut(s) 569
BstBI TTCGAA 1 cut(s) 16
BstC8I GCNNGC 2 cut(s) 574, 596
BstDEI CTNAG 1 cut(s) 638
BstF5I GGATG 2 cut(s) 214, 235
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 1 cut(s) 223
BstMBI GATC 1 cut(s) 220
BstMWI GCNNNNNNNGC 2 cut(s) 100, 569
BstNI CCWGG 3 cut(s) 164, 418, 554
BstNSI RCATGY 2 cut(s) 200, 572
BstSCI CCNGG 3 cut(s) 162, 416, 552
BstX2I RGATCY 1 cut(s) 220
BstYI RGATCY 1 cut(s) 220
BsuRI GGCC 1 cut(s) 63
BtrI CACGTC 1 cut(s) 361
BtsCI GGATG 2 cut(s) 214, 235
BtsIMutI CAGTG 2 cut(s) 343, 468
Cac8I GCNNGC 2 cut(s) 574, 596
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 211, 458
CseI GACGC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 432, 621
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 3 cut(s) 38, 197, 569
CviJI RGCY 9 cut(s) 63, 78, 157, 167, 382, 416, 549, 576, 598
CviKI_1 RGCY 9 cut(s) 63, 78, 157, 167, 382, 416, 549, 576, 598
CviQI GTAC 2 cut(s) 432, 621
DdeI CTNAG 1 cut(s) 638
DpnI GATC 1 cut(s) 222
DpnII GATC 1 cut(s) 220
EaeI YGGCCR 1 cut(s) 61
Eam1104I CTCTTC 2 cut(s) 147, 273
EarI CTCTTC 2 cut(s) 147, 273
Ecl136II GAGCTC 1 cut(s) 157
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 2 cut(s) 211, 458
Eco53kI GAGCTC 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 157
EcoRII CCWGG 3 cut(s) 162, 416, 552
EcoT38I GRGCYC 1 cut(s) 159
FaeI CATG 3 cut(s) 41, 200, 572
FalI AAGNNNNNCTT 2 cut(s) 67, 99
FaqI GGGAC 1 cut(s) 354
FatI CATG 3 cut(s) 37, 196, 568
FokI GGATG 2 cut(s) 201, 242
FriOI GRGCYC 1 cut(s) 159
FspBI CTAG 2 cut(s) 87, 144
FspI TGCGCA 1 cut(s) 189
GlaI GCGC 1 cut(s) 189
HaeIII GGCC 1 cut(s) 63
HapII CCGG 1 cut(s) 64
HgaI GACGC 1 cut(s) 83
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 94
Hin1II CATG 3 cut(s) 41, 200, 572
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HindIII AAGCTT 2 cut(s) 380, 596
HinfI GANTC 1 cut(s) 250
HpaII CCGG 1 cut(s) 64
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 2 cut(s) 458, 631
Hpy188I TCNGA 1 cut(s) 34
Hpy188III TCNNGA 4 cut(s) 209, 218, 227, 339
Hpy8I GTNNAC 2 cut(s) 458, 631
Hpy99I CGWCG 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 8 cut(s) 103, 125, 173, 364, 392, 496, 517, 572
HpyF10VI GCNNNNNNNGC 2 cut(s) 100, 569
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 1 cut(s) 94
Hsp92II CATG 3 cut(s) 41, 200, 572
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 1 cut(s) 220
LguI GCTCTTC 1 cut(s) 147
LweI GCATC 2 cut(s) 112, 199
MaeI CTAG 2 cut(s) 87, 144
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 4 cut(s) 43, 347, 356, 396
MalI GATC 1 cut(s) 222
MboI GATC 1 cut(s) 220
MboII GAAGA 3 cut(s) 164, 215, 290
MflI RGATCY 1 cut(s) 220
MhlI GDGCHC 2 cut(s) 30, 159
MluCI AATT 6 cut(s) 11, 204, 256, 312, 325, 481
MnlI CCTC 5 cut(s) 60, 63, 84, 188, 274
MslI CAYNNNNRTG 1 cut(s) 195
MspI CCGG 1 cut(s) 64
MspR9I CCNGG 3 cut(s) 164, 418, 554
MvaI CCWGG 3 cut(s) 164, 418, 554
MwoI GCNNNNNNNGC 2 cut(s) 100, 569
NdeII GATC 1 cut(s) 220
NlaIII CATG 3 cut(s) 41, 200, 572
NmuCI GTSAC 2 cut(s) 356, 396
NsbI TGCGCA 1 cut(s) 189
NspI RCATGY 2 cut(s) 200, 572
NspV TTCGAA 1 cut(s) 16
PciI ACATGT 1 cut(s) 196
PciSI GCTCTTC 1 cut(s) 147
PfeI GAWTC 1 cut(s) 250
PscI ACATGT 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 159
Psp6I CCWGG 3 cut(s) 162, 416, 552
PspGI CCWGG 3 cut(s) 162, 416, 552
PspPI GGNCC 2 cut(s) 211, 458
PsuI RGATCY 1 cut(s) 220
RsaI GTAC 2 cut(s) 433, 622
RsaNI GTAC 2 cut(s) 432, 621
RseI CAYNNNNRTG 1 cut(s) 195
SacI GAGCTC 1 cut(s) 159
SapI GCTCTTC 1 cut(s) 147
Sau3AI GATC 1 cut(s) 220
Sau96I GGNCC 2 cut(s) 211, 458
ScaI AGTACT 1 cut(s) 433
ScrFI CCNGG 3 cut(s) 164, 418, 554
SduI GDGCHC 2 cut(s) 30, 159
SfaNI GCATC 2 cut(s) 112, 199
SfuI TTCGAA 1 cut(s) 16
SinI GGWCC 2 cut(s) 211, 458
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 6 cut(s) 11, 204, 256, 312, 325, 481
SsiI CCGC 1 cut(s) 445
SspMI CTAG 2 cut(s) 87, 144
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 3 cut(s) 162, 416, 552
TaaI ACNGT 1 cut(s) 347
TaiI ACGT 1 cut(s) 363
TaqI TCGA 1 cut(s) 16
TasI AATT 6 cut(s) 11, 204, 256, 312, 325, 481
TatI WGTACW 1 cut(s) 431
TfiI GAWTC 1 cut(s) 250
TscAI CASTG 2 cut(s) 350, 468
TseFI GTSAC 2 cut(s) 356, 396
Tsp45I GTSAC 2 cut(s) 356, 396
TspDTI ATGAA 2 cut(s) 522, 546
TspGWI ACGGA 1 cut(s) 384
TspRI CASTG 2 cut(s) 350, 468
VpaK11BI GGWCC 2 cut(s) 211, 458
XapI RAATTY 2 cut(s) 11, 204
XceI RCATGY 2 cut(s) 200, 572
XcmI CCANNNNNNNNNTGG 1 cut(s) 468
XspI CTAG 2 cut(s) 87, 144
ZrmI AGTACT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.