RLG00000003534

N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001436
Physical Location & Seq
Reverse (-)
49760425 .. 49770446
10022 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003534

Sequence Viewer

Length: 1155 bp
ATGGGTGATAACCATTTTGTTGTTGTTGTTGTGGGTATTGATATTGAGGAGGGTAGTAAGATTGGTGGGTATTGGTATTGTAATGGTAGTGTGGTTGTTGTGGGGGTTGGAAGTGTTGTTGTTGGTGGTGGTGTTGGTGTTGGGCTTGGAGGAGCTGCTGTTGGTGAAGCTGTTGGAGGTGGTGATGGTCCATTTTTGTACGGGAAGAAAGATTGGGGCTTCGGGTTTTCTGGGCCGGAGGGGTTTGGGCGTTTGAACGGAAAAAGAGGGAGAGCGAAGAGGAAGAGAGGACCGGAGCTTTGTTGCTTGCGATTTGCGAATTTGGATTTTGATCCCTTTGGTGCAACAAGACCGAGGACTGCGTTGGCCGGAGATGCAGTTTTGATCGTGTCGTCCTCTCAAATCTATAACCCAGCTTCTCTGTTCAAAATTCTCCATGGTTCATACTCACCATCTAAGGGCGCTTTCTTGAATGATTCCAATAATGGTGGCAGTGCCAAGAAGAGAAATGTGTTGCTGGTTGTTGCTCACCCAGATGACGAGTCCATGTTCTTCACTCCAACAATAAACTACCTCACTTCGAGAGGGCATAATATTCACATCTTATGCTTGTCAATTGGTGATGCAGATGGTAAAGGAAATACTAGAAAAGAAGAGCTCTACAAGGCTTCTGCAACCCTCAAGGTTCCACATCAACAAGTGAAGATTCTGGACCATCCAGATCTTCAGGATGGTTTTGGCAAGGTGTGGAATCACAATTTATTGGCAAAGATTATTGAAGAGGAAGTCGTAAGTCAGGGCATTGACTTGATAATCACTTTTGATAACTATGGAGTTTCGGGACACTGTAACCATTGTGACGTACATTATGGAGTAAGGAAGCTTTTACTTGCATCGTCACAGAGGAATGTTGAAGCCTGGGAACTTGTCAGTACTAACATATTCCGCAAGTATAGTGGACCAGTGGATATATGGTTCTCCAATTTATATTTTATGCAATGCTCAGATGAAATGTTGCATTGCTTGGTAAACGAGCAGCCCCGAAAGAGTTTCCTCGCGATGGCACAACATGCAAGCCAGTGGGTTTGGTTTCGCAAGCTTTTTGTGGCATTTTCGAGCTGCACCTATGTGAATACGCTTAGAAAGATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PIG-L

Protein Analysis

385

Amino Acids

41.99

Weight (kDa)

8.62

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIG-L PF02585 172 - 294 1.4e-21 GlcNAc-PI de-N-acetylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1058
AciI CCGC 1 cut(s) 946
AclWI GGATC 1 cut(s) 326
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 2 cut(s) 319, 429
AcuI CTGAAG 1 cut(s) 710
AfaI GTAC 3 cut(s) 200, 864, 934
AfiI CCNNNNNNNGG 2 cut(s) 458, 1060
AgsI TTSAA 5 cut(s) 256, 427, 472, 779, 914
AjnI CCWGG 1 cut(s) 917
AleI CACNNNNGTG 1 cut(s) 1127
AloI GAACNNNNNNTCC 2 cut(s) 959, 991
AluBI AGCT 8 cut(s) 155, 170, 298, 416, 658, 883, 1099, 1119
AluI AGCT 8 cut(s) 155, 170, 298, 416, 658, 883, 1099, 1119
Alw21I GWGCWC 1 cut(s) 660
AlwI GGATC 1 cut(s) 326
AoxI GGCC 2 cut(s) 233, 366
ApeKI GCWGC 3 cut(s) 155, 1036, 1119
ApoI RAATTY 2 cut(s) 319, 429
ArsI GACNNNNNNTTYG 2 cut(s) 282, 314
AspLEI GCGC 1 cut(s) 464
AspS9I GGNCC 5 cut(s) 188, 233, 290, 712, 959
AsuHPI GGTGA 6 cut(s) 17, 176, 194, 441, 521, 632
AvaII GGWCC 4 cut(s) 188, 290, 712, 959
BanII GRGCYC 1 cut(s) 660
Bbv12I GWGCWC 1 cut(s) 660
BbvI GCAGC 3 cut(s) 142, 1048, 1106
BccI CCATC 6 cut(s) 179, 460, 623, 723, 725, 1054
BciT130I CCWGG 1 cut(s) 919
BfaI CTAG 1 cut(s) 645
BfoI RGCGCY 1 cut(s) 465
BglII AGATCT 1 cut(s) 721
BisI GCNGC 3 cut(s) 156, 1037, 1120
BlsI GCNGC 3 cut(s) 157, 1038, 1121
BmcAI AGTACT 1 cut(s) 934
Bme1390I CCNGG 1 cut(s) 919
Bme18I GGWCC 4 cut(s) 188, 290, 712, 959
BmgT120I GGNCC 5 cut(s) 188, 233, 290, 712, 959
BmiI GGNNCC 1 cut(s) 687
BmrFI CCNGG 1 cut(s) 919
BmsI GCATC 3 cut(s) 364, 613, 902
BpuEI CTTGAG 1 cut(s) 665
BsaBI GATNNNNATC 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 353, 436, 918
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 4 cut(s) 144, 174, 363, 393
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 1060
Bse1I ACTGG 2 cut(s) 962, 1078
Bse3DI GCAATG 2 cut(s) 1004, 1018
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 1 cut(s) 919
BseDI CCNNGG 3 cut(s) 353, 436, 918
BseGI GGATG 2 cut(s) 715, 736
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 2 cut(s) 458, 1060
BseMI GCAATG 2 cut(s) 1004, 1018
BseMII CTCAG 1 cut(s) 1017
BseNI ACTGG 2 cut(s) 962, 1078
BseRI GAGGAG 2 cut(s) 62, 165
BseXI GCAGC 3 cut(s) 142, 1048, 1106
BseYI CCCAGC 1 cut(s) 412
BsgI GTGCAG 1 cut(s) 1105
Bsh1236I CGCG 1 cut(s) 1058
BshFI GGCC 2 cut(s) 235, 368
BsiHKAI GWGCWC 1 cut(s) 660
BsiSI CCGG 3 cut(s) 236, 293, 369
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 2 cut(s) 458, 1060
BsmFI GGGAC 1 cut(s) 855
BsnI GGCC 2 cut(s) 235, 368
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 3 cut(s) 331, 384, 721
Bsp19I CCATGG 1 cut(s) 436
Bsp68I TCGCGA 1 cut(s) 1058
BspACI CCGC 1 cut(s) 946
BspANI GGCC 2 cut(s) 235, 368
BspCNI CTCAG 1 cut(s) 1016
BspFNI CGCG 1 cut(s) 1058
BspLI GGNNCC 1 cut(s) 687
BspPI GGATC 1 cut(s) 326
BspQI GCTCTTC 1 cut(s) 648
BsrDI GCAATG 2 cut(s) 1004, 1018
BsrI ACTGG 2 cut(s) 962, 1078
BssECI CCNNGG 3 cut(s) 353, 436, 918
BssMI GATC 3 cut(s) 331, 384, 721
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 919
Bst4CI ACNGT 1 cut(s) 848
Bst6I CTCTTC 5 cut(s) 272, 278, 497, 648, 774
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 3 cut(s) 308, 1075, 1097
BstDEI CTNAG 3 cut(s) 456, 1003, 1139
BstDSI CCRYGG 1 cut(s) 436
BstF5I GGATG 2 cut(s) 715, 736
BstFNI CGCG 1 cut(s) 1058
BstH2I RGCGCY 1 cut(s) 465
BstHHI GCGC 1 cut(s) 464
BstKTI GATC 3 cut(s) 334, 387, 724
BstMBI GATC 3 cut(s) 331, 384, 721
BstMWI GCNNNNNNNGC 2 cut(s) 374, 1070
BstNI CCWGG 1 cut(s) 919
BstNSI RCATGY 1 cut(s) 1073
BstSCI CCNGG 1 cut(s) 917
BstUI CGCG 1 cut(s) 1058
BstV1I GCAGC 3 cut(s) 142, 1048, 1106
BstX2I RGATCY 1 cut(s) 721
BstYI RGATCY 1 cut(s) 721
BsuRI GGCC 2 cut(s) 235, 368
BtgI CCRYGG 1 cut(s) 436
BtgZI GCGATG 1 cut(s) 1073
BtsCI GGATG 2 cut(s) 715, 736
BtsI GCAGTG 1 cut(s) 499
BtsIMutI CAGTG 4 cut(s) 499, 844, 969, 1085
BtuMI TCGCGA 1 cut(s) 1058
Cac8I GCNNGC 3 cut(s) 308, 1075, 1097
CfoI GCGC 1 cut(s) 464
Cfr13I GGNCC 5 cut(s) 188, 233, 290, 712, 959
Csp6I GTAC 3 cut(s) 199, 863, 933
CspCI CAANNNNNGTGG 4 cut(s) 469, 504, 937, 972
CviAII CATG 3 cut(s) 437, 547, 1070
CviQI GTAC 3 cut(s) 199, 863, 933
DdeI CTNAG 3 cut(s) 456, 1003, 1139
DpnI GATC 3 cut(s) 333, 386, 723
DpnII GATC 3 cut(s) 331, 384, 721
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 5 cut(s) 272, 278, 497, 648, 774
EarI CTCTTC 5 cut(s) 272, 278, 497, 648, 774
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 660
Eco47I GGWCC 4 cut(s) 188, 290, 712, 959
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 710
EcoICRI GAGCTC 1 cut(s) 658
EcoRII CCWGG 1 cut(s) 917
EcoT14I CCWWGG 1 cut(s) 436
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 3 cut(s) 440, 550, 1073
FaqI GGGAC 1 cut(s) 855
FatI CATG 3 cut(s) 436, 546, 1069
Fnu4HI GCNGC 3 cut(s) 156, 1037, 1120
FokI GGATG 2 cut(s) 702, 743
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 3 cut(s) 156, 1037, 1120
FspBI CTAG 1 cut(s) 645
GlaI GCGC 1 cut(s) 463
GluI GCNGC 3 cut(s) 156, 1037, 1120
GsaI CCCAGC 1 cut(s) 416
HaeII RGCGCY 1 cut(s) 465
HaeIII GGCC 2 cut(s) 235, 368
HapII CCGG 3 cut(s) 236, 293, 369
HhaI GCGC 1 cut(s) 464
Hin1II CATG 3 cut(s) 440, 550, 1073
Hin6I GCGC 1 cut(s) 462
HinP1I GCGC 1 cut(s) 462
HindIII AAGCTT 2 cut(s) 881, 1097
HinfI GANTC 4 cut(s) 476, 542, 706, 751
HpaII CCGG 3 cut(s) 236, 293, 369
HphI GGTGA 6 cut(s) 17, 176, 194, 441, 521, 632
Hpy166II GTNNAC 2 cut(s) 959, 1030
Hpy188I TCNGA 1 cut(s) 1006
Hpy188III TCNNGA 7 cut(s) 469, 582, 710, 719, 728, 840, 1057
Hpy8I GTNNAC 2 cut(s) 959, 1030
HpyCH4III ACNGT 1 cut(s) 848
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 9 cut(s) 344, 377, 626, 674, 893, 997, 1018, 1073, 1122
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 1070
HpyF3I CTNAG 3 cut(s) 456, 1003, 1139
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 3 cut(s) 440, 550, 1073
HspAI GCGC 1 cut(s) 462
Kzo9I GATC 3 cut(s) 331, 384, 721
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 2 cut(s) 152, 295
Lsp1109I GCAGC 3 cut(s) 142, 1048, 1106
LweI GCATC 3 cut(s) 364, 613, 902
MaeI CTAG 1 cut(s) 645
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 3 cut(s) 848, 857, 897
MalI GATC 3 cut(s) 333, 386, 723
MboI GATC 3 cut(s) 331, 384, 721
MboII GAAGA 9 cut(s) 217, 289, 295, 514, 544, 665, 715, 716, 791
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 721
MhlI GDGCHC 1 cut(s) 660
MluCI AATT 5 cut(s) 319, 429, 615, 757, 982
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 3 cut(s) 88, 154, 584
MslI CAYNNNNRTG 2 cut(s) 534, 1127
MspI CCGG 3 cut(s) 236, 293, 369
MspR9I CCNGG 1 cut(s) 919
MunI CAATTG 1 cut(s) 615
MvaI CCWGG 1 cut(s) 919
MvnI CGCG 1 cut(s) 1058
MwoI GCNNNNNNNGC 2 cut(s) 374, 1070
NcoI CCATGG 1 cut(s) 436
NdeII GATC 3 cut(s) 331, 384, 721
NlaIII CATG 3 cut(s) 440, 550, 1073
NlaIV GGNNCC 1 cut(s) 687
NmuCI GTSAC 2 cut(s) 857, 897
NruI TCGCGA 1 cut(s) 1058
NspI RCATGY 1 cut(s) 1073
OliI CACNNNNGTG 1 cut(s) 1127
PciSI GCTCTTC 1 cut(s) 648
PfeI GAWTC 3 cut(s) 476, 706, 751
PkrI GCNGC 3 cut(s) 157, 1038, 1121
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 917
PspFI CCCAGC 1 cut(s) 412
PspGI CCWGG 1 cut(s) 917
PspN4I GGNNCC 1 cut(s) 687
PspPI GGNCC 5 cut(s) 188, 233, 290, 712, 959
PsuI RGATCY 1 cut(s) 721
RruI TCGCGA 1 cut(s) 1058
RsaI GTAC 3 cut(s) 200, 864, 934
RsaNI GTAC 3 cut(s) 199, 863, 933
RseI CAYNNNNRTG 2 cut(s) 534, 1127
SacI GAGCTC 1 cut(s) 660
SapI GCTCTTC 1 cut(s) 648
SatI GCNGC 3 cut(s) 156, 1037, 1120
Sau3AI GATC 3 cut(s) 331, 384, 721
Sau96I GGNCC 5 cut(s) 188, 233, 290, 712, 959
ScaI AGTACT 1 cut(s) 934
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 919
SduI GDGCHC 1 cut(s) 660
SfaNI GCATC 3 cut(s) 364, 613, 902
SinI GGWCC 4 cut(s) 188, 290, 712, 959
SmiMI CAYNNNNRTG 2 cut(s) 534, 1127
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 5 cut(s) 319, 429, 615, 757, 982
SsiI CCGC 1 cut(s) 946
SspI AATATT 1 cut(s) 595
SspMI CTAG 1 cut(s) 645
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 917
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 1 cut(s) 848
TaiI ACGT 1 cut(s) 864
TaqI TCGA 2 cut(s) 581, 1115
TaqII GACCGA 1 cut(s) 367
TasI AATT 5 cut(s) 319, 429, 615, 757, 982
TatI WGTACW 1 cut(s) 932
TfiI GAWTC 3 cut(s) 476, 706, 751
TscAI CASTG 4 cut(s) 499, 851, 969, 1085
TseFI GTSAC 2 cut(s) 857, 897
TseI GCWGC 3 cut(s) 155, 1036, 1119
Tsp45I GTSAC 2 cut(s) 857, 897
TspDTI ATGAA 2 cut(s) 432, 1023
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 4 cut(s) 499, 851, 969, 1085
VpaK11BI GGWCC 4 cut(s) 188, 290, 712, 959
XapI RAATTY 2 cut(s) 319, 429
XceI RCATGY 1 cut(s) 1073
XcmI CCANNNNNNNNNTGG 1 cut(s) 969
XspI CTAG 1 cut(s) 645
ZrmI AGTACT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.