AT3G62770

Autophagy-related protein 18a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001367
Physical Location & Seq
Reverse (-)
23218610 .. 23221243
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62770.1

Sequence Viewer

Length: 1278 bp
ATGGCCACCGTATCTTCTTCCTCCTGGCCAAACCCCAACCCTAATCCCGATTCCACGTCTGCCTCAGATTCCGATTCTACTTTTCCCTCTCACCGCGATCGCGTAGACGAACCCGACTCTCTCGATTCCTTCTCCTCCATGAGTCTTAACTCCGACGAACCTAATCAGACTTCTAATCAATCGCCTCTTTCTCCCCCTACGCCCAATTTACCGGTGATGCCTCCTCCGTCCGTGCTTCATCTTTCCTTTAACCAAGATCATGCTTGCTTCGCTGTCGGCACTGACCGTGGCTTCCGGATCCTTAATTGCGATCCCTTTCGCGAGATTTTCCGGCGTGATTTCGATCGTGGCGGTGGTGTTGCAGTCGTGGAGATGCTTTTCAGATGCAATATATTAGCCCTAGTTGGTGGCGGACCTGATCCTCAATATCCTCCTAATAAGGTTATGATTTGGGATGATCACCAGGGCCGATGTATCGGAGAACTCTCTTTCAGGTCCGATGTTAGATCCGTCCGGCTTAGGAGGGATCGGATTATTGTCGTTCTTGAGCAGAAGATTTTTGTCTACAATTTCTCTGACCTCAAGCTGATGCATCAGATTGAAACCATTGCCAACCCTAAGGGTTTGTGTGCTGTTTCTCAGGGTGTTGGTTCTATGGTTTTGGTATGTCCAGGTTTGCAGAAAGGTCAAGTTCGGATCGAGCACTACGCTTCTAAACGGACCAAATTCGTCATGGCTCATGATTCCAGAATAGCTTGCTTCGCTCTCACGCAGGATGGCCATTTGTTGGCCACTGCTAGCTCTAAGGGTACTCTGGTTCGGATCTTCAATACTGTTGATGGTACCTTGCGTCAAGAGGTAAGGAGGGGTGCGGATAGAGCAGAGATCTACAGTTTGGCCTTCTCTTCAAATGCTCAGTGGTTAGCTGTCTCAAGTGACAAAGGAACGGTCCATGTCTTTGGTCTCAAAGTCAACTCCGGATCTCAAGTGAAAGACTCATCCCGAATTGCACCTGATGCTACTCCCTCATCCCCATCGTCGTCTCTGTCTTTATTCAAAGGAGTGTTACCGAGGTATTTCAGCTCGGAGTGGTCGGTGGCTCAGTTCAGGTTGGTTGAAGGAACTCAGTACATAGCCGCCTTTGGCCATCAAAAGAACACCGTTGTTATTCTTGGCATGGATGGGAGCTTCTACAGATGCCAGTTTGATCCGGTGAACGGCGGTGAAATGTCTCAGCTTGAGTACCACAACTGTCTGAAACCGCCTTCAGTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000045 GO:0000407 GO:0000422 GO:0001101 GO:0003674 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006497 GO:0006807 GO:0006914 GO:0006950 GO:0006952 GO:0006970 GO:0006979 GO:0006996 GO:0007005 GO:0007033 GO:0007275 GO:0007568 GO:0008104 GO:0008150 GO:0008152 GO:0008289 GO:0009056 GO:0009058 GO:0009059 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009620 GO:0009628 GO:0009651 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0009991 GO:0010035 GO:0010150 GO:0010506 GO:0010508 GO:0016020 GO:0016043 GO:0016236 GO:0019222 GO:0019538 GO:0019898 GO:0022411 GO:0022607 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031667 GO:0032266 GO:0032501 GO:0032502 GO:0033036 GO:0034045 GO:0034497 GO:0034613 GO:0034645 GO:0035091 GO:0036211 GO:0042157 GO:0042158 GO:0042221 GO:0042594 GO:0043167 GO:0043168 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044267 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0044804 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051179 GO:0051641 GO:0051704 GO:0051707 GO:0061726 GO:0061919 GO:0065007 GO:0070727 GO:0070925 GO:0071704 GO:0071840 GO:0080025 GO:0090693 GO:0098542 GO:0098805 GO:0099402 GO:1901564 GO:1901566 GO:1901576 GO:1901700 GO:1901981 GO:1902936 GO:1903008 GO:1905037
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

425

Amino Acids

46.8

Weight (kDa)

7.15

Isoelectric Point (pI)

51.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROPPIN PF21032 81 - 336 2e-35 PROPPIN
Beta-prop_THOC3 PF25174 81 - 330 3e-13 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 175 - 325 7.7e-08 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 183 - 325 1.8e-07 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 229 - 323 4.3e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 237 - 323 7.9e-08 Prp19 WD40 domain
WD40_WDHD1_1st PF24817 238 - 333 3.5e-06 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 246 - 323 2.3e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 842
AccB1I GGYRCC 1 cut(s) 842
AccB7I CCANNNNNTGG 1 cut(s) 787
AccI GTMKAC 2 cut(s) 105, 564
AccII CGCG 3 cut(s) 96, 102, 321
AccIII TCCGGA 2 cut(s) 294, 977
AciI CCGC 7 cut(s) 94, 351, 411, 872, 1137, 1221, 1262
AcoI YGGCCR 5 cut(s) 3, 26, 778, 789, 1144
AcsI RAATTY 1 cut(s) 725
AcuI CTGAAG 1 cut(s) 1251
AfaI GTAC 4 cut(s) 811, 844, 1130, 1244
AfiI CCNNNNNNNGG 2 cut(s) 787, 1217
AgeI ACCGGT 1 cut(s) 211
AgsI TTSAA 5 cut(s) 602, 829, 909, 1057, 1118
AjiI CACGTC 1 cut(s) 57
AjnI CCWGG 3 cut(s) 23, 462, 670
AluBI AGCT 7 cut(s) 586, 755, 801, 926, 1083, 1188, 1237
AluI AGCT 7 cut(s) 586, 755, 801, 926, 1083, 1188, 1237
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 4 cut(s) 934, 968, 1047, 1236
Aor13HI TCCGGA 2 cut(s) 294, 977
AoxI GGCC 7 cut(s) 3, 26, 466, 778, 789, 897, 1144
ApoI RAATTY 1 cut(s) 725
ArsI GACNNNNNNTTYG 2 cut(s) 933, 965
AsiGI ACCGGT 1 cut(s) 211
AsiSI GCGATCGC 1 cut(s) 100
Asp718I GGTACC 1 cut(s) 842
AspS9I GGNCC 5 cut(s) 413, 466, 495, 720, 949
AsuHPI GGTGA 5 cut(s) 83, 226, 452, 1225, 1235
AsuNHI GCTAGC 1 cut(s) 797
AvaII GGWCC 4 cut(s) 413, 495, 720, 949
AxyI CCTNAGG 1 cut(s) 618
BaeI ACNNNNGTAYC 2 cut(s) 801, 834
BalI TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
BamHI GGATCC 1 cut(s) 297
BanI GGYRCC 1 cut(s) 842
Bbv12I GWGCWC 1 cut(s) 705
BccI CCATC 5 cut(s) 770, 833, 1042, 1155, 1175
BceAI ACGGC 1 cut(s) 1234
BciT130I CCWGG 3 cut(s) 25, 464, 672
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 4 cut(s) 934, 968, 1047, 1236
BfaI CTAG 2 cut(s) 401, 798
BfmI CTRYAG 2 cut(s) 889, 1192
BglII AGATCT 1 cut(s) 885
BisI GCNGC 1 cut(s) 1137
BlsI GCNGC 1 cut(s) 1138
Bme1390I CCNGG 3 cut(s) 25, 464, 672
Bme18I GGWCC 4 cut(s) 413, 495, 720, 949
BmgBI CACGTC 1 cut(s) 57
BmgT120I GGNCC 5 cut(s) 413, 466, 495, 720, 949
BmiI GGNNCC 2 cut(s) 299, 844
BmrFI CCNGG 3 cut(s) 25, 464, 672
BmsI GCATC 7 cut(s) 207, 363, 374, 579, 601, 1006, 1187
BmtI GCTAGC 1 cut(s) 801
Bpu10I CCTNAGC 1 cut(s) 518
BpuEI CTTGAG 5 cut(s) 566, 566, 916, 969, 1259
BsaBI GATNNNNATC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 968
BsaJI CCNNGG 3 cut(s) 286, 463, 1070
BsaWI WCCGGW 4 cut(s) 211, 294, 977, 1210
Bsc4I CCNNNNNNNGG 2 cut(s) 787, 1217
Bse118I RCCGGY 1 cut(s) 211
Bse1I ACTGG 1 cut(s) 1201
Bse21I CCTNAGG 1 cut(s) 618
Bse3DI GCAATG 1 cut(s) 606
Bse8I GATNNNNATC 1 cut(s) 342
BseAI TCCGGA 2 cut(s) 294, 977
BseBI CCWGG 3 cut(s) 25, 464, 672
BseDI CCNNGG 3 cut(s) 286, 463, 1070
BseGI GGATG 5 cut(s) 460, 781, 998, 1028, 1186
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 2 cut(s) 787, 1217
BseMI GCAATG 1 cut(s) 606
BseMII CTCAG 6 cut(s) 78, 653, 929, 1115, 1139, 1247
BseNI ACTGG 1 cut(s) 1201
BseRI GAGGAG 2 cut(s) 124, 213
Bsh1236I CGCG 3 cut(s) 96, 102, 321
Bsh1285I CGRYCG 2 cut(s) 100, 346
BshFI GGCC 7 cut(s) 5, 28, 468, 780, 791, 899, 1146
BshNI GGYRCC 1 cut(s) 842
BshTI ACCGGT 1 cut(s) 211
BsiEI CGRYCG 2 cut(s) 100, 346
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 6 cut(s) 212, 295, 331, 514, 978, 1211
BslI CCNNNNNNNGG 2 cut(s) 787, 1217
BsmAI GTCTC 4 cut(s) 934, 968, 1047, 1236
BsmBI CGTCTC 1 cut(s) 1047
BsnI GGCC 7 cut(s) 5, 28, 468, 780, 791, 899, 1146
Bso31I GGTCTC 1 cut(s) 968
Bsp1286I GDGCHC 1 cut(s) 705
Bsp13I TCCGGA 2 cut(s) 294, 977
Bsp68I TCGCGA 1 cut(s) 321
BspACI CCGC 7 cut(s) 94, 351, 411, 872, 1137, 1221, 1262
BspANI GGCC 7 cut(s) 5, 28, 468, 780, 791, 899, 1146
BspCNI CTCAG 6 cut(s) 77, 652, 928, 1114, 1138, 1246
BspEI TCCGGA 2 cut(s) 294, 977
BspFNI CGCG 3 cut(s) 96, 102, 321
BspHI TCATGA 1 cut(s) 739
BspLI GGNNCC 2 cut(s) 299, 844
BspOI GCTAGC 1 cut(s) 801
BspT107I GGYRCC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 968
BsrDI GCAATG 1 cut(s) 606
BsrFI RCCGGY 1 cut(s) 211
BsrI ACTGG 1 cut(s) 1201
BssAI RCCGGY 1 cut(s) 211
BssECI CCNNGG 3 cut(s) 286, 463, 1070
Bst2UI CCWGG 3 cut(s) 25, 464, 672
Bst4CI ACNGT 7 cut(s) 10, 287, 835, 893, 949, 1162, 1253
Bst6I CTCTTC 1 cut(s) 910
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstC8I GCNNGC 3 cut(s) 265, 757, 799
BstDEI CTNAG 9 cut(s) 64, 518, 618, 639, 804, 915, 1101, 1125, 1233
BstDSI CCRYGG 1 cut(s) 286
BstF5I GGATG 5 cut(s) 460, 781, 998, 1028, 1186
BstFNI CGCG 3 cut(s) 96, 102, 321
BstMAI GTCTC 4 cut(s) 934, 968, 1047, 1236
BstMCI CGRYCG 2 cut(s) 100, 346
BstMWI GCNNNNNNNGC 4 cut(s) 269, 761, 878, 1016
BstNI CCWGG 3 cut(s) 25, 464, 672
BstSCI CCNGG 3 cut(s) 23, 462, 670
BstSFI CTRYAG 2 cut(s) 889, 1192
BstUI CGCG 3 cut(s) 96, 102, 321
BstX2I RGATCY 5 cut(s) 297, 506, 822, 885, 980
BstXI CCANNNNNNTGG 1 cut(s) 959
BstYI RGATCY 5 cut(s) 297, 506, 822, 885, 980
Bsu36I CCTNAGG 1 cut(s) 618
BsuRI GGCC 7 cut(s) 5, 28, 468, 780, 791, 899, 1146
BtgI CCRYGG 1 cut(s) 286
BtrI CACGTC 1 cut(s) 57
BtsCI GGATG 5 cut(s) 460, 781, 998, 1028, 1186
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 3 cut(s) 279, 792, 923
BtuMI TCGCGA 1 cut(s) 321
Cac8I GCNNGC 3 cut(s) 265, 757, 799
CciI TCATGA 1 cut(s) 739
Cfr10I RCCGGY 1 cut(s) 211
Cfr13I GGNCC 5 cut(s) 413, 466, 495, 720, 949
CseI GACGC 1 cut(s) 839
Csp6I GTAC 4 cut(s) 810, 843, 1129, 1243
CspAI ACCGGT 1 cut(s) 211
CviAII CATG 6 cut(s) 139, 260, 733, 740, 953, 1177
CviQI GTAC 4 cut(s) 810, 843, 1129, 1243
DdeI CTNAG 9 cut(s) 64, 518, 618, 639, 804, 915, 1101, 1125, 1233
EaeI YGGCCR 5 cut(s) 3, 26, 778, 789, 1144
Eam1104I CTCTTC 1 cut(s) 910
EarI CTCTTC 1 cut(s) 910
EciI GGCGGA 1 cut(s) 426
Eco31I GGTCTC 1 cut(s) 968
Eco47I GGWCC 4 cut(s) 413, 495, 720, 949
Eco57I CTGAAG 1 cut(s) 1251
Eco81I CCTNAGG 1 cut(s) 618
EcoRII CCWGG 3 cut(s) 23, 462, 670
EcoT22I ATGCAT 1 cut(s) 594
Esp3I CGTCTC 1 cut(s) 1047
FaeI CATG 6 cut(s) 142, 263, 736, 743, 956, 1180
FatI CATG 6 cut(s) 138, 259, 732, 739, 952, 1176
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 2 cut(s) 105, 564
Fnu4HI GCNGC 1 cut(s) 1137
FokI GGATG 5 cut(s) 467, 788, 985, 1015, 1193
Fsp4HI GCNGC 1 cut(s) 1137
FspBI CTAG 2 cut(s) 401, 798
GluI GCNGC 1 cut(s) 1137
HaeIII GGCC 7 cut(s) 5, 28, 468, 780, 791, 899, 1146
HapII CCGG 6 cut(s) 212, 295, 331, 514, 978, 1211
HgaI GACGC 1 cut(s) 839
Hin1II CATG 6 cut(s) 142, 263, 736, 743, 956, 1180
HincII GTYRAC 1 cut(s) 973
HindII GTYRAC 1 cut(s) 973
HinfI GANTC 8 cut(s) 50, 68, 74, 116, 125, 142, 743, 995
HpaII CCGG 6 cut(s) 212, 295, 331, 514, 978, 1211
HphI GGTGA 5 cut(s) 83, 226, 452, 1225, 1235
Hpy166II GTNNAC 4 cut(s) 106, 565, 973, 1216
Hpy8I GTNNAC 4 cut(s) 106, 565, 973, 1216
Hpy99I CGWCG 2 cut(s) 158, 1042
HpyAV CCTTC 4 cut(s) 139, 910, 1112, 1275
HpyCH4III ACNGT 7 cut(s) 10, 287, 835, 893, 949, 1162, 1253
HpyCH4IV ACGT 1 cut(s) 56
HpyCH4V TGCA 5 cut(s) 362, 387, 592, 679, 1010
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 761, 878, 1016
HpyF3I CTNAG 9 cut(s) 64, 518, 618, 639, 804, 915, 1101, 1125, 1233
HpySE526I ACGT 1 cut(s) 56
Hsp92II CATG 6 cut(s) 142, 263, 736, 743, 956, 1180
Kpn2I TCCGGA 2 cut(s) 294, 977
KpnI GGTACC 1 cut(s) 846
Ksp22I TGATCA 1 cut(s) 457
LmnI GCTCC 1 cut(s) 1185
LweI GCATC 7 cut(s) 207, 363, 374, 579, 601, 1006, 1187
MaeI CTAG 2 cut(s) 401, 798
MaeII ACGT 1 cut(s) 56
MaeIII GTNAC 2 cut(s) 935, 1065
MboII GAAGA 5 cut(s) 6, 9, 565, 817, 897
MflI RGATCY 5 cut(s) 297, 506, 822, 885, 980
MhlI GDGCHC 1 cut(s) 705
MlsI TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
MluCI AATT 5 cut(s) 205, 304, 568, 725, 1005
MluNI TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
MlyI GAGTC 3 cut(s) 110, 151, 989
MmeI TCCRAC 1 cut(s) 177
Mox20I TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
Mph1103I ATGCAT 1 cut(s) 594
MroI TCCGGA 2 cut(s) 294, 977
MscI TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
MseI TTAA 3 cut(s) 147, 249, 303
Msp20I TGGCCA 5 cut(s) 5, 28, 780, 791, 1146
MspI CCGG 6 cut(s) 212, 295, 331, 514, 978, 1211
MspR9I CCNGG 3 cut(s) 25, 464, 672
MvaI CCWGG 3 cut(s) 25, 464, 672
MvnI CGCG 3 cut(s) 96, 102, 321
MwoI GCNNNNNNNGC 4 cut(s) 269, 761, 878, 1016
NheI GCTAGC 1 cut(s) 797
NlaIII CATG 6 cut(s) 142, 263, 736, 743, 956, 1180
NlaIV GGNNCC 2 cut(s) 299, 844
NmuCI GTSAC 1 cut(s) 935
NruI TCGCGA 1 cut(s) 321
NsiI ATGCAT 1 cut(s) 594
PagI TCATGA 1 cut(s) 739
PfeI GAWTC 5 cut(s) 50, 68, 74, 125, 743
PflMI CCANNNNNTGG 1 cut(s) 787
PinAI ACCGGT 1 cut(s) 211
PkrI GCNGC 1 cut(s) 1138
Ple19I CGATCG 2 cut(s) 100, 346
PleI GAGTC 3 cut(s) 110, 150, 989
PpsI GAGTC 3 cut(s) 110, 150, 989
Psp6I CCWGG 3 cut(s) 23, 462, 670
PspGI CCWGG 3 cut(s) 23, 462, 670
PspN4I GGNNCC 2 cut(s) 299, 844
PspPI GGNCC 5 cut(s) 413, 466, 495, 720, 949
PsuI RGATCY 5 cut(s) 297, 506, 822, 885, 980
PvuI CGATCG 2 cut(s) 100, 346
RgaI GCGATCGC 1 cut(s) 100
RruI TCGCGA 1 cut(s) 321
RsaI GTAC 4 cut(s) 811, 844, 1130, 1244
RsaNI GTAC 4 cut(s) 810, 843, 1129, 1243
SaqAI TTAA 3 cut(s) 147, 249, 303
SatI GCNGC 1 cut(s) 1137
Sau96I GGNCC 5 cut(s) 413, 466, 495, 720, 949
SchI GAGTC 3 cut(s) 110, 151, 989
ScrFI CCNGG 3 cut(s) 25, 464, 672
SduI GDGCHC 1 cut(s) 705
SfaAI GCGATCGC 1 cut(s) 100
SfaNI GCATC 7 cut(s) 207, 363, 374, 579, 601, 1006, 1187
SfcI CTRYAG 2 cut(s) 889, 1192
SgfI GCGATCGC 1 cut(s) 100
SinI GGWCC 4 cut(s) 413, 495, 720, 949
SmlI CTYRAG 5 cut(s) 545, 581, 931, 984, 1238
SmoI CTYRAG 5 cut(s) 545, 581, 931, 984, 1238
Sse9I AATT 5 cut(s) 205, 304, 568, 725, 1005
SsiI CCGC 7 cut(s) 94, 351, 411, 872, 1137, 1221, 1262
SspMI CTAG 2 cut(s) 401, 798
StyD4I CCNGG 3 cut(s) 23, 462, 670
TaaI ACNGT 7 cut(s) 10, 287, 835, 893, 949, 1162, 1253
TaiI ACGT 1 cut(s) 59
TaqI TCGA 3 cut(s) 123, 342, 699
TasI AATT 5 cut(s) 205, 304, 568, 725, 1005
TatI WGTACW 1 cut(s) 1128
TauI GCSGC 1 cut(s) 1139
TfiI GAWTC 5 cut(s) 50, 68, 74, 125, 743
Tru1I TTAA 3 cut(s) 147, 249, 303
Tru9I TTAA 3 cut(s) 147, 249, 303
TscAI CASTG 3 cut(s) 286, 799, 923
TseFI GTSAC 1 cut(s) 935
Tsp45I GTSAC 1 cut(s) 935
TspDTI ATGAA 1 cut(s) 227
TspGWI ACGGA 4 cut(s) 216, 220, 499, 733
TspRI CASTG 3 cut(s) 286, 799, 923
Van91I CCANNNNNTGG 1 cut(s) 787
VpaK11BI GGWCC 4 cut(s) 413, 495, 720, 949
XapI RAATTY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XmiI GTMKAC 2 cut(s) 105, 564
XspI CTAG 2 cut(s) 401, 798
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.