Rh3DG069200

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0001367
Physical Location & Seq
Forward (+)
4710894 .. 4715422
4529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG069200.1

Sequence Viewer

Length: 717 bp
ATGGCCACCGTCTCCTCCCCTCCTTTTCAGTTTCCGGTCCCCAAACTCGACCCCGAACCCGACCCGACCCGACTCCCCACCATCTTCAACCTCTCCTTCAACCAGGACCACGCCTGCTTCGCCGTCGGCACCGACTTCGGCTTCCGCGTCTACAAGTGCGACCCCTTCGCCATCACCATCCGCCGCGACTTCTTCACAGGCGGCGGCTTCGGATTGGTCCGGATACTCAACACCTCCCAAATCTTCGCCATAGTCGGAGGCGGACCCGACCCGGTCTCCCCGGCCAACAGGGTCATGATCTGGGACGACCACAAGTCCCTCCCCATCCACGACATGTCGTTTCGCTCTGAGGTCAGGGGGCTCAAGCTCCGGAGCGATCGACTCGTCGTCGTTTTGAACCAGAAGATCGTCGTCCACAGCTTCCCTAATTCGAAGCCTCTGCACGAGATTTTGACCACGGAGAACCCTAAGGGGCTGTGCGAGGTGTCGCAGAGTCCGGGTCCGATGGTGCTGGCGTGTCCGGGGCTCCATAAAGGTCAAATCCGGGTCGAGAATTACGCTTTGAAGCGGACCAAGTTCATAATGGCCCACGATTCCAGTCTCGCCTGCTTTGGGCTCACTCCGGACGGCCGGTATTTGGCTACTGCGAGCAGCAAAGGGACTCTAGTTAGAGTTTTCAGTACTTCGGATGGTACATTACTCCAAGAGGTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

238

Amino Acids

26.18

Weight (kDa)

8.85

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROPPIN PF21032 31 - 236 2.2e-18 PROPPIN
Beta-prop_THOC3 PF25174 96 - 235 3e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 150
AccII CGCG 2 cut(s) 147, 186
AccIII TCCGGA 3 cut(s) 219, 369, 622
AciI CCGC 7 cut(s) 145, 181, 184, 201, 204, 261, 568
AcoI YGGCCR 3 cut(s) 3, 282, 628
AfaI GTAC 3 cut(s) 682, 694, 711
AfiI CCNNNNNNNGG 2 cut(s) 612, 637
AflIII ACRYGT 1 cut(s) 333
AgsI TTSAA 4 cut(s) 88, 100, 397, 565
AhdI GACNNNNNGTC 2 cut(s) 313, 386
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 2 cut(s) 367, 420
AluI AGCT 2 cut(s) 367, 420
Alw26I GTCTC 3 cut(s) 16, 280, 605
Aor13HI TCCGGA 3 cut(s) 219, 369, 622
AoxI GGCC 4 cut(s) 3, 282, 585, 628
ApeKI GCWGC 1 cut(s) 651
AspS9I GGNCC 7 cut(s) 37, 106, 217, 263, 500, 570, 586
AsuC2I CCSGG 5 cut(s) 272, 281, 498, 522, 545
AsuHPI GGTGA 1 cut(s) 166
AsuII TTCGAA 1 cut(s) 431
AvaII GGWCC 6 cut(s) 37, 106, 217, 263, 500, 570
AxyI CCTNAGG 1 cut(s) 468
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 3 cut(s) 363, 528, 618
BauI CACGAG 1 cut(s) 443
BbvI GCAGC 1 cut(s) 663
BccI CCATC 6 cut(s) 89, 179, 185, 332, 499, 683
BceAI ACGGC 2 cut(s) 107, 643
BcgI CGANNNNNNTGC 4 cut(s) 118, 152, 421, 455
BciT130I CCWGG 1 cut(s) 104
BciVI GTATCC 1 cut(s) 216
BcnI CCSGG 5 cut(s) 272, 281, 498, 522, 545
BcoDI GTCTC 3 cut(s) 16, 280, 605
BfaI CTAG 1 cut(s) 665
BfuI GTATCC 1 cut(s) 216
BisI GCNGC 4 cut(s) 184, 202, 205, 652
BlsI GCNGC 4 cut(s) 185, 203, 206, 653
BmcAI AGTACT 1 cut(s) 682
Bme1390I CCNGG 6 cut(s) 104, 272, 281, 498, 522, 545
Bme18I GGWCC 6 cut(s) 37, 106, 217, 263, 500, 570
BmeRI GACNNNNNGTC 2 cut(s) 313, 386
BmgT120I GGNCC 7 cut(s) 37, 106, 217, 263, 500, 570, 586
BmiI GGNNCC 5 cut(s) 39, 130, 265, 501, 527
BmrFI CCNGG 6 cut(s) 104, 272, 281, 498, 522, 545
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 347
BpuMI CCSGG 5 cut(s) 272, 281, 498, 522, 545
BsaI GGTCTC 1 cut(s) 280
BsaJI CCNNGG 3 cut(s) 279, 456, 521
BsaWI WCCGGW 4 cut(s) 34, 219, 369, 622
BsaXI ACNNNNNCTCC 2 cut(s) 260, 290
Bsc4I CCNNNNNNNGG 2 cut(s) 612, 637
Bse118I RCCGGY 1 cut(s) 630
Bse1I ACTGG 1 cut(s) 597
Bse21I CCTNAGG 1 cut(s) 468
BseAI TCCGGA 3 cut(s) 219, 369, 622
BseBI CCWGG 1 cut(s) 104
BseDI CCNNGG 3 cut(s) 279, 456, 521
BseGI GGATG 3 cut(s) 177, 324, 694
BseLI CCNNNNNNNGG 2 cut(s) 612, 637
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 597
BseRI GAGGAG 1 cut(s) 4
BseX3I CGGCCG 1 cut(s) 628
BseXI GCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 425
Bsh1236I CGCG 2 cut(s) 147, 186
Bsh1285I CGRYCG 2 cut(s) 379, 631
BshFI GGCC 4 cut(s) 5, 284, 587, 630
BshNI GGYRCC 1 cut(s) 128
BsiEI CGRYCG 2 cut(s) 379, 631
BslFI GGGAC 4 cut(s) 23, 301, 317, 673
BslI CCNNNNNNNGG 2 cut(s) 612, 637
BsmAI GTCTC 3 cut(s) 16, 280, 605
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 4 cut(s) 23, 301, 317, 673
BsnI GGCC 4 cut(s) 5, 284, 587, 630
Bso31I GGTCTC 1 cut(s) 280
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 363, 528, 618
Bsp13I TCCGGA 3 cut(s) 219, 369, 622
Bsp143I GATC 3 cut(s) 297, 376, 405
BspACI CCGC 7 cut(s) 145, 181, 184, 201, 204, 261, 568
BspANI GGCC 4 cut(s) 5, 284, 587, 630
BspCNI CTCAG 1 cut(s) 340
BspEI TCCGGA 3 cut(s) 219, 369, 622
BspFNI CGCG 2 cut(s) 147, 186
BspHI TCATGA 1 cut(s) 294
BspLI GGNNCC 5 cut(s) 39, 130, 265, 501, 527
BspT104I TTCGAA 1 cut(s) 431
BspT107I GGYRCC 1 cut(s) 128
BspTNI GGTCTC 1 cut(s) 280
BsrFI RCCGGY 1 cut(s) 630
BsrI ACTGG 1 cut(s) 597
BssAI RCCGGY 1 cut(s) 630
BssECI CCNNGG 3 cut(s) 279, 456, 521
BssMI GATC 3 cut(s) 297, 376, 405
BssSI CACGAG 1 cut(s) 443
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 1 cut(s) 10
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 4 cut(s) 115, 513, 607, 649
BstDEI CTNAG 2 cut(s) 348, 468
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 3 cut(s) 177, 324, 694
BstFNI CGCG 2 cut(s) 147, 186
BstKTI GATC 3 cut(s) 300, 379, 408
BstMAI GTCTC 3 cut(s) 16, 280, 605
BstMBI GATC 3 cut(s) 297, 376, 405
BstMCI CGRYCG 2 cut(s) 379, 631
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 337
BstSCI CCNGG 6 cut(s) 102, 270, 279, 496, 520, 543
BstUI CGCG 2 cut(s) 147, 186
BstV1I GCAGC 1 cut(s) 663
BstZI CGGCCG 1 cut(s) 628
Bsu36I CCTNAGG 1 cut(s) 468
BsuI GTATCC 1 cut(s) 216
BsuRI GGCC 4 cut(s) 5, 284, 587, 630
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 3 cut(s) 177, 324, 694
Cac8I GCNNGC 4 cut(s) 115, 513, 607, 649
CciI TCATGA 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 630
Cfr13I GGNCC 7 cut(s) 37, 106, 217, 263, 500, 570, 586
CseI GACGC 1 cut(s) 136
Csp6I GTAC 3 cut(s) 681, 693, 710
CviAII CATG 2 cut(s) 295, 334
CviQI GTAC 3 cut(s) 681, 693, 710
DdeI CTNAG 2 cut(s) 348, 468
DpnI GATC 3 cut(s) 299, 378, 407
DpnII GATC 3 cut(s) 297, 376, 405
DriI GACNNNNNGTC 2 cut(s) 313, 386
EaeI YGGCCR 3 cut(s) 3, 282, 628
EagI CGGCCG 1 cut(s) 628
Eam1105I GACNNNNNGTC 2 cut(s) 313, 386
EciI GGCGGA 2 cut(s) 170, 276
EclXI CGGCCG 1 cut(s) 628
Eco24I GRGCYC 3 cut(s) 363, 528, 618
Eco31I GGTCTC 1 cut(s) 280
Eco47I GGWCC 6 cut(s) 37, 106, 217, 263, 500, 570
Eco52I CGGCCG 1 cut(s) 628
Eco81I CCTNAGG 1 cut(s) 468
EcoRII CCWGG 1 cut(s) 102
EcoT38I GRGCYC 3 cut(s) 363, 528, 618
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 2 cut(s) 298, 337
FaiI YATR 5 cut(s) 251, 296, 335, 531, 581
FalI AAGNNNNNCTT 1 cut(s) 696
FaqI GGGAC 4 cut(s) 23, 301, 317, 673
FatI CATG 2 cut(s) 294, 333
FblI GTMKAC 1 cut(s) 150
Fnu4HI GCNGC 4 cut(s) 184, 202, 205, 652
FokI GGATG 3 cut(s) 164, 311, 701
FriOI GRGCYC 3 cut(s) 363, 528, 618
Fsp4HI GCNGC 4 cut(s) 184, 202, 205, 652
FspBI CTAG 1 cut(s) 665
GluI GCNGC 4 cut(s) 184, 202, 205, 652
HaeIII GGCC 4 cut(s) 5, 284, 587, 630
HgaI GACGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 298, 337
HinfI GANTC 5 cut(s) 72, 381, 493, 593, 661
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 2 cut(s) 151, 415
Hpy188I TCNGA 5 cut(s) 212, 257, 349, 504, 688
Hpy188III TCNNGA 5 cut(s) 220, 295, 370, 550, 623
Hpy8I GTNNAC 2 cut(s) 151, 415
Hpy99I CGWCG 4 cut(s) 128, 389, 392, 413
HpyAV CCTTC 2 cut(s) 106, 175
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4V TGCA 1 cut(s) 442
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpyF3I CTNAG 2 cut(s) 348, 468
Hsp92II CATG 2 cut(s) 298, 337
Kpn2I TCCGGA 3 cut(s) 219, 369, 622
Kzo9I GATC 3 cut(s) 297, 376, 405
LmnI GCTCC 3 cut(s) 372, 372, 531
Lsp1109I GCAGC 1 cut(s) 663
MaeI CTAG 1 cut(s) 665
MalI GATC 3 cut(s) 299, 378, 407
MboI GATC 3 cut(s) 297, 376, 405
MboII GAAGA 4 cut(s) 76, 184, 235, 415
MhlI GDGCHC 3 cut(s) 363, 528, 618
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 427, 553
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 4 cut(s) 66, 375, 502, 655
MmeI TCCRAC 1 cut(s) 235
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 3 cut(s) 219, 369, 622
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 6 cut(s) 104, 272, 281, 498, 522, 545
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 2 cut(s) 147, 186
MwoI GCNNNNNNNGC 1 cut(s) 119
NciI CCSGG 5 cut(s) 272, 281, 498, 522, 545
NdeII GATC 3 cut(s) 297, 376, 405
NlaIII CATG 2 cut(s) 298, 337
NlaIV GGNNCC 5 cut(s) 39, 130, 265, 501, 527
NspI RCATGY 1 cut(s) 337
NspV TTCGAA 1 cut(s) 431
PagI TCATGA 1 cut(s) 294
PciI ACATGT 1 cut(s) 333
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PfeI GAWTC 1 cut(s) 593
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 185, 203, 206, 653
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 4 cut(s) 66, 375, 501, 655
PpsI GAGTC 4 cut(s) 66, 375, 501, 655
PscI ACATGT 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 5 cut(s) 39, 130, 265, 501, 527
PspPI GGNCC 7 cut(s) 37, 106, 217, 263, 500, 570, 586
PsyI GACNNNGTC 1 cut(s) 272
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 3 cut(s) 682, 694, 711
RsaNI GTAC 3 cut(s) 681, 693, 710
SatI GCNGC 4 cut(s) 184, 202, 205, 652
Sau3AI GATC 3 cut(s) 297, 376, 405
Sau96I GGNCC 7 cut(s) 37, 106, 217, 263, 500, 570, 586
ScaI AGTACT 1 cut(s) 682
SchI GAGTC 4 cut(s) 66, 375, 502, 655
ScrFI CCNGG 6 cut(s) 104, 272, 281, 498, 522, 545
SduI GDGCHC 3 cut(s) 363, 528, 618
SetI ASST 8 cut(s) 93, 236, 354, 369, 422, 486, 538, 711
SfuI TTCGAA 1 cut(s) 431
SinI GGWCC 6 cut(s) 37, 106, 217, 263, 500, 570
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 2 cut(s) 427, 553
SsiI CCGC 7 cut(s) 145, 181, 184, 201, 204, 261, 568
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 6 cut(s) 102, 270, 279, 496, 520, 543
TaaI ACNGT 1 cut(s) 10
TaqI TCGA 4 cut(s) 48, 379, 431, 549
TasI AATT 2 cut(s) 427, 553
TatI WGTACW 1 cut(s) 680
TauI GCSGC 3 cut(s) 186, 204, 207
TfiI GAWTC 1 cut(s) 593
TseI GCWGC 1 cut(s) 651
TspDTI ATGAA 1 cut(s) 568
TspGWI ACGGA 1 cut(s) 473
Tth111I GACNNNGTC 1 cut(s) 272
VpaK11BI GGWCC 6 cut(s) 37, 106, 217, 263, 500, 570
XceI RCATGY 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 150
XspI CTAG 1 cut(s) 665
ZrmI AGTACT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.