Rroxscaffold_6G00424290

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0001367
Physical Location & Seq
Reverse (-)
45438308 .. 45441578
3271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424290.1

Sequence Viewer

Length: 1158 bp
ATGATCTGGGACGACCACAAGTCCCTCCGCATCCACGACATGTCGTTTCGCTCTGAGGTCAGGGGGCTCAAGCTCCGGAGCGATCGACTCGTCGTCGTTTTGAACCAGAAGATCATCGTCCACAGCTTCCCTAATTCGAAGCCTCTGCACGAGATACTGACCACGGAGAACCCTAAGGGGCTGTGCGAGGTGTCGCAGAGTCCGGGTCCGATGGTGCTGGCGTGTCCGGGGCTCCATAAGGGTCAAATTCGGGTCGAGAATTACGCATTGAAGCGGACCAAGTTCATAATGGCCCACGATTCCAGTCTCGCCTGCTTTGGGCTCACTCAGGACGGCCGGTATTTGGCTACGGCGAGCAGCAAAGGGACTCTAGTTAGAGTTTTCAGTACTTCGGATGGTACATTACTCCAAGAGGTGAGGAGAGGAGCAGACAGAGCAGAGATTTACAATCTTGCTTTCTCTTGTACTGGCCAATGGCTAGCTGTGTCTAGTGACAAGGGAACTGTTCATGTTTTCAGTGTAAAGGTTGGTTCAGGATCCATGGAAAAGGATAGATCACGCGGTACATCAGAAGGCGGTGTTCCTAATCCATCAGGCATTTCATCTCTGTCTTTCATCAAAGGTGTGCTGCCAAGGTATTTCAGCTCAGAATGGTCAGTGGCCAAATTTCACTTGCAGGAAGGTCTGCAGCATATTGTTGCCTTTGGCAAGCGGAAGAACACCATCATGATTGTTGGCATGGATGGAAGAGAAATCCCATCATCATTGACTAGTCTGAATTTTTTGTCATATCTCAATGTCTCCTACAACAATCTGGAAGGGCAGCAAATACCGAAAGGCACTCAGTTCCAAGGTTTTGATGTATCTGCATTTGAAGCGAACCCGAAACTTTGCGGTCTTCCACATCCAAATGAGTGCCAAGCAATTAACGGATTTGATCAAGATGTGGACAACGAGCATCAAACTCCATGGCTTTATGTTTATGCTGCGCTTGGGTTCATTGTAGGATTCTGGGGAGTCTGTAGTTCATTGATTTTCAACAAGACATGGAGATATGCATTCTTCCGATTTCTAGAAAATCTACAAGATATGGTACATGTAATGACGGCTGTGCGTTTCACAAAGATGAAGAGAAGGCTTTATGCTAGCTATACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

385

Amino Acids

43.11

Weight (kDa)

9.22

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 2 - 178 6.4e-12 THOC3 beta-propeller domain
PROPPIN PF21032 7 - 180 9e-26 PROPPIN
Beta-prop_EML_2 PF23414 82 - 176 8.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 98 - 177 2.4e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 561
AccIII TCCGGA 1 cut(s) 75
AciI CCGC 6 cut(s) 28, 274, 561, 576, 712, 894
AclWI GGATC 2 cut(s) 531, 544
AcoI YGGCCR 3 cut(s) 334, 469, 660
AcsI RAATTY 3 cut(s) 246, 665, 778
AfaI GTAC 5 cut(s) 388, 400, 466, 565, 1095
AfiI CCNNNNNNNGG 2 cut(s) 318, 343
AflIII ACRYGT 2 cut(s) 39, 1096
AgsI TTSAA 4 cut(s) 103, 271, 875, 1039
AhdI GACNNNNNGTC 2 cut(s) 19, 92
AhlI ACTAGT 1 cut(s) 770
AluBI AGCT 5 cut(s) 73, 126, 482, 645, 1149
AluI AGCT 5 cut(s) 73, 126, 482, 645, 1149
Alw26I GTCTC 2 cut(s) 311, 805
AlwI GGATC 2 cut(s) 531, 544
Aor13HI TCCGGA 1 cut(s) 75
AoxI GGCC 4 cut(s) 291, 334, 469, 660
ApeKI GCWGC 5 cut(s) 357, 628, 688, 823, 986
ApoI RAATTY 3 cut(s) 246, 665, 778
AspLEI GCGC 1 cut(s) 991
AspS9I GGNCC 3 cut(s) 206, 276, 292
AsuC2I CCSGG 2 cut(s) 204, 228
AsuHPI GGTGA 1 cut(s) 427
AsuII TTCGAA 1 cut(s) 137
AsuNHI GCTAGC 2 cut(s) 478, 1145
AvaII GGWCC 2 cut(s) 206, 276
AxyI CCTNAGG 1 cut(s) 174
BalI TGGCCA 2 cut(s) 471, 662
BamHI GGATCC 1 cut(s) 536
BanII GRGCYC 3 cut(s) 69, 234, 324
BauI CACGAG 1 cut(s) 149
BbsI GAAGAC 1 cut(s) 890
BbvI GCAGC 5 cut(s) 369, 615, 700, 835, 973
BccI CCATC 6 cut(s) 205, 389, 598, 731, 737, 766
BceAI ACGGC 3 cut(s) 349, 366, 1122
BcgI CGANNNNNNTGC 2 cut(s) 127, 161
BclI TGATCA 1 cut(s) 937
BcnI CCSGG 2 cut(s) 204, 228
BcoDI GTCTC 2 cut(s) 311, 805
BcuI ACTAGT 1 cut(s) 770
BfaI CTAG 6 cut(s) 371, 479, 489, 771, 1073, 1146
BfmI CTRYAG 2 cut(s) 686, 1021
BisI GCNGC 5 cut(s) 358, 629, 689, 824, 987
BlsI GCNGC 5 cut(s) 359, 630, 690, 825, 988
BmcAI AGTACT 1 cut(s) 388
Bme1390I CCNGG 2 cut(s) 204, 228
Bme18I GGWCC 2 cut(s) 206, 276
BmeRI GACNNNNNGTC 2 cut(s) 19, 92
BmgT120I GGNCC 3 cut(s) 206, 276, 292
BmiI GGNNCC 3 cut(s) 207, 233, 538
BmrFI CCNGG 2 cut(s) 204, 228
BmsI GCATC 2 cut(s) 39, 967
BmtI GCTAGC 2 cut(s) 482, 1149
BpiI GAAGAC 1 cut(s) 890
Bpu14I TTCGAA 1 cut(s) 137
BpuEI CTTGAG 1 cut(s) 53
BpuMI CCSGG 2 cut(s) 204, 228
BsaJI CCNNGG 6 cut(s) 162, 227, 540, 632, 850, 968
BsaWI WCCGGW 1 cut(s) 75
Bsc4I CCNNNNNNNGG 2 cut(s) 318, 343
Bse118I RCCGGY 1 cut(s) 336
Bse1I ACTGG 2 cut(s) 303, 472
Bse21I CCTNAGG 1 cut(s) 174
BseAI TCCGGA 1 cut(s) 75
BseDI CCNNGG 6 cut(s) 162, 227, 540, 632, 850, 968
BseGI GGATG 4 cut(s) 30, 400, 748, 904
BseLI CCNNNNNNNGG 2 cut(s) 318, 343
BseMII CTCAG 4 cut(s) 45, 341, 660, 857
BseNI ACTGG 2 cut(s) 303, 472
BseRI GAGGAG 2 cut(s) 433, 438
BseX3I CGGCCG 1 cut(s) 334
BseXI GCAGC 5 cut(s) 369, 615, 700, 835, 973
BsgI GTGCAG 1 cut(s) 131
Bsh1236I CGCG 1 cut(s) 561
Bsh1285I CGRYCG 2 cut(s) 85, 337
BshFI GGCC 4 cut(s) 293, 336, 471, 662
BsiEI CGRYCG 2 cut(s) 85, 337
BsiSI CCGG 4 cut(s) 76, 203, 227, 337
BslFI GGGAC 3 cut(s) 7, 23, 379
BslI CCNNNNNNNGG 2 cut(s) 318, 343
BsmAI GTCTC 2 cut(s) 311, 805
BsmFI GGGAC 3 cut(s) 7, 23, 379
BsmI GAATGC 1 cut(s) 1058
BsnI GGCC 4 cut(s) 293, 336, 471, 662
Bsp119I TTCGAA 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 69, 234, 324
Bsp13I TCCGGA 1 cut(s) 75
Bsp143I GATC 6 cut(s) 3, 82, 111, 536, 554, 937
Bsp19I CCATGG 2 cut(s) 540, 968
BspACI CCGC 6 cut(s) 28, 274, 561, 576, 712, 894
BspANI GGCC 4 cut(s) 293, 336, 471, 662
BspCNI CTCAG 4 cut(s) 46, 340, 659, 856
BspEI TCCGGA 1 cut(s) 75
BspFNI CGCG 1 cut(s) 561
BspHI TCATGA 1 cut(s) 726
BspLI GGNNCC 3 cut(s) 207, 233, 538
BspMAI CTGCAG 1 cut(s) 690
BspOI GCTAGC 2 cut(s) 482, 1149
BspPI GGATC 2 cut(s) 531, 544
BspT104I TTCGAA 1 cut(s) 137
BsrFI RCCGGY 1 cut(s) 336
BsrI ACTGG 2 cut(s) 303, 472
BssAI RCCGGY 1 cut(s) 336
BssECI CCNNGG 6 cut(s) 162, 227, 540, 632, 850, 968
BssMI GATC 6 cut(s) 3, 82, 111, 536, 554, 937
BssSI CACGAG 1 cut(s) 149
BssT1I CCWWGG 4 cut(s) 540, 632, 850, 968
Bst2BI CACGAG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 2 cut(s) 742, 1124
BstBI TTCGAA 1 cut(s) 137
BstC8I GCNNGC 6 cut(s) 219, 313, 355, 480, 710, 1147
BstDEI CTNAG 5 cut(s) 54, 174, 327, 646, 843
BstDSI CCRYGG 3 cut(s) 162, 540, 968
BstF5I GGATG 4 cut(s) 30, 400, 748, 904
BstFNI CGCG 1 cut(s) 561
BstHHI GCGC 1 cut(s) 991
BstKTI GATC 6 cut(s) 6, 85, 114, 539, 557, 940
BstMAI GTCTC 2 cut(s) 311, 805
BstMBI GATC 6 cut(s) 3, 82, 111, 536, 554, 937
BstMCI CGRYCG 2 cut(s) 85, 337
BstMWI GCNNNNNNNGC 2 cut(s) 434, 875
BstNSI RCATGY 2 cut(s) 43, 1100
BstSCI CCNGG 2 cut(s) 202, 226
BstSFI CTRYAG 2 cut(s) 686, 1021
BstUI CGCG 1 cut(s) 561
BstV1I GCAGC 5 cut(s) 369, 615, 700, 835, 973
BstV2I GAAGAC 1 cut(s) 890
BstX2I RGATCY 1 cut(s) 536
BstYI RGATCY 1 cut(s) 536
BstZI CGGCCG 1 cut(s) 334
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 4 cut(s) 293, 336, 471, 662
BtgI CCRYGG 3 cut(s) 162, 540, 968
BtsCI GGATG 4 cut(s) 30, 400, 748, 904
BtsIMutI CAGTG 2 cut(s) 523, 663
Cac8I GCNNGC 6 cut(s) 219, 313, 355, 480, 710, 1147
CciI TCATGA 1 cut(s) 726
CfoI GCGC 1 cut(s) 991
Cfr10I RCCGGY 1 cut(s) 336
Cfr13I GGNCC 3 cut(s) 206, 276, 292
Csp6I GTAC 5 cut(s) 387, 399, 465, 564, 1094
CviAII CATG 8 cut(s) 40, 509, 541, 727, 739, 969, 1047, 1097
CviQI GTAC 5 cut(s) 387, 399, 465, 564, 1094
DdeI CTNAG 5 cut(s) 54, 174, 327, 646, 843
DpnI GATC 6 cut(s) 5, 84, 113, 538, 556, 939
DpnII GATC 6 cut(s) 3, 82, 111, 536, 554, 937
DriI GACNNNNNGTC 2 cut(s) 19, 92
EaeI YGGCCR 3 cut(s) 334, 469, 660
EagI CGGCCG 1 cut(s) 334
Eam1104I CTCTTC 2 cut(s) 742, 1124
Eam1105I GACNNNNNGTC 2 cut(s) 19, 92
EarI CTCTTC 2 cut(s) 742, 1124
EclXI CGGCCG 1 cut(s) 334
Eco130I CCWWGG 4 cut(s) 540, 632, 850, 968
Eco24I GRGCYC 3 cut(s) 69, 234, 324
Eco47I GGWCC 2 cut(s) 206, 276
Eco52I CGGCCG 1 cut(s) 334
Eco81I CCTNAGG 1 cut(s) 174
EcoT14I CCWWGG 4 cut(s) 540, 632, 850, 968
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 3 cut(s) 69, 234, 324
ErhI CCWWGG 4 cut(s) 540, 632, 850, 968
FaeI CATG 8 cut(s) 43, 512, 544, 730, 742, 972, 1050, 1100
FaqI GGGAC 3 cut(s) 7, 23, 379
FatI CATG 8 cut(s) 39, 508, 540, 726, 738, 968, 1046, 1096
FbaI TGATCA 1 cut(s) 937
Fnu4HI GCNGC 5 cut(s) 358, 629, 689, 824, 987
FokI GGATG 4 cut(s) 17, 407, 755, 891
FriOI GRGCYC 3 cut(s) 69, 234, 324
Fsp4HI GCNGC 5 cut(s) 358, 629, 689, 824, 987
FspBI CTAG 6 cut(s) 371, 479, 489, 771, 1073, 1146
GlaI GCGC 1 cut(s) 990
GluI GCNGC 5 cut(s) 358, 629, 689, 824, 987
HaeIII GGCC 4 cut(s) 293, 336, 471, 662
HapII CCGG 4 cut(s) 76, 203, 227, 337
HhaI GCGC 1 cut(s) 991
Hin1II CATG 8 cut(s) 43, 512, 544, 730, 742, 972, 1050, 1100
Hin6I GCGC 1 cut(s) 989
HinP1I GCGC 1 cut(s) 989
HinfI GANTC 6 cut(s) 87, 199, 299, 367, 1008, 1017
HpaII CCGG 4 cut(s) 76, 203, 227, 337
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 2 cut(s) 121, 949
Hpy188I TCNGA 7 cut(s) 55, 210, 394, 571, 649, 777, 1067
Hpy188III TCNNGA 8 cut(s) 76, 256, 329, 534, 727, 815, 941, 1073
Hpy8I GTNNAC 2 cut(s) 121, 949
Hpy99I CGWCG 2 cut(s) 95, 98
HpyAV CCTTC 4 cut(s) 566, 674, 812, 1128
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4V TGCA 5 cut(s) 148, 676, 688, 869, 1058
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 875
HpyF3I CTNAG 5 cut(s) 54, 174, 327, 646, 843
Hsp92II CATG 8 cut(s) 43, 512, 544, 730, 742, 972, 1050, 1100
HspAI GCGC 1 cut(s) 989
Kpn2I TCCGGA 1 cut(s) 75
Ksp22I TGATCA 1 cut(s) 937
Kzo9I GATC 6 cut(s) 3, 82, 111, 536, 554, 937
LmnI GCTCC 4 cut(s) 78, 78, 237, 425
Lsp1109I GCAGC 5 cut(s) 369, 615, 700, 835, 973
LweI GCATC 2 cut(s) 39, 967
MaeI CTAG 6 cut(s) 371, 479, 489, 771, 1073, 1146
MaeIII GTNAC 1 cut(s) 491
MalI GATC 6 cut(s) 5, 84, 113, 538, 556, 939
MboI GATC 6 cut(s) 3, 82, 111, 536, 554, 937
MboII GAAGA 6 cut(s) 121, 727, 759, 890, 1054, 1141
MflI RGATCY 1 cut(s) 536
MhlI GDGCHC 3 cut(s) 69, 234, 324
MlsI TGGCCA 2 cut(s) 471, 662
MluCI AATT 6 cut(s) 133, 246, 259, 665, 778, 924
MluNI TGGCCA 2 cut(s) 471, 662
MlyI GAGTC 4 cut(s) 81, 208, 361, 1026
MnlI CCTC 7 cut(s) 35, 49, 153, 181, 406, 411, 416
Mox20I TGGCCA 2 cut(s) 471, 662
Mph1103I ATGCAT 1 cut(s) 1060
MroI TCCGGA 1 cut(s) 75
MscI TGGCCA 2 cut(s) 471, 662
MseI TTAA 1 cut(s) 927
MslI CAYNNNNRTG 3 cut(s) 725, 909, 1124
Msp20I TGGCCA 2 cut(s) 471, 662
MspI CCGG 4 cut(s) 76, 203, 227, 337
MspR9I CCNGG 2 cut(s) 204, 228
Mva1269I GAATGC 1 cut(s) 1058
MvnI CGCG 1 cut(s) 561
MwoI GCNNNNNNNGC 2 cut(s) 434, 875
NciI CCSGG 2 cut(s) 204, 228
NcoI CCATGG 2 cut(s) 540, 968
NdeII GATC 6 cut(s) 3, 82, 111, 536, 554, 937
NheI GCTAGC 2 cut(s) 478, 1145
NlaIII CATG 8 cut(s) 43, 512, 544, 730, 742, 972, 1050, 1100
NlaIV GGNNCC 3 cut(s) 207, 233, 538
NmuCI GTSAC 1 cut(s) 491
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 2 cut(s) 43, 1100
NspV TTCGAA 1 cut(s) 137
PagI TCATGA 1 cut(s) 726
PciI ACATGT 2 cut(s) 39, 1096
PctI GAATGC 1 cut(s) 1058
PfeI GAWTC 2 cut(s) 299, 1008
PkrI GCNGC 5 cut(s) 359, 630, 690, 825, 988
Ple19I CGATCG 1 cut(s) 85
PleI GAGTC 4 cut(s) 81, 207, 361, 1025
PpsI GAGTC 4 cut(s) 81, 207, 361, 1025
PscI ACATGT 2 cut(s) 39, 1096
PspN4I GGNNCC 3 cut(s) 207, 233, 538
PspPI GGNCC 3 cut(s) 206, 276, 292
PstI CTGCAG 1 cut(s) 690
PsuI RGATCY 1 cut(s) 536
PvuI CGATCG 1 cut(s) 85
RsaI GTAC 5 cut(s) 388, 400, 466, 565, 1095
RsaNI GTAC 5 cut(s) 387, 399, 465, 564, 1094
RseI CAYNNNNRTG 3 cut(s) 725, 909, 1124
SaqAI TTAA 1 cut(s) 927
SatI GCNGC 5 cut(s) 358, 629, 689, 824, 987
Sau3AI GATC 6 cut(s) 3, 82, 111, 536, 554, 937
Sau96I GGNCC 3 cut(s) 206, 276, 292
ScaI AGTACT 1 cut(s) 388
SchI GAGTC 4 cut(s) 81, 208, 361, 1026
ScrFI CCNGG 2 cut(s) 204, 228
SduI GDGCHC 3 cut(s) 69, 234, 324
SfaNI GCATC 2 cut(s) 39, 967
SfcI CTRYAG 2 cut(s) 686, 1021
SfuI TTCGAA 1 cut(s) 137
SinI GGWCC 2 cut(s) 206, 276
SmiMI CAYNNNNRTG 3 cut(s) 725, 909, 1124
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 770
Sse9I AATT 6 cut(s) 133, 246, 259, 665, 778, 924
SsiI CCGC 6 cut(s) 28, 274, 561, 576, 712, 894
SspMI CTAG 6 cut(s) 371, 479, 489, 771, 1073, 1146
StyD4I CCNGG 2 cut(s) 202, 226
StyI CCWWGG 4 cut(s) 540, 632, 850, 968
TaaI ACNGT 1 cut(s) 505
TaqI TCGA 3 cut(s) 85, 137, 255
TasI AATT 6 cut(s) 133, 246, 259, 665, 778, 924
TatI WGTACW 2 cut(s) 386, 464
TfiI GAWTC 2 cut(s) 299, 1008
Tru1I TTAA 1 cut(s) 927
Tru9I TTAA 1 cut(s) 927
TscAI CASTG 2 cut(s) 523, 663
TseFI GTSAC 1 cut(s) 491
TseI GCWGC 5 cut(s) 357, 628, 688, 823, 986
Tsp45I GTSAC 1 cut(s) 491
TspDTI ATGAA 7 cut(s) 274, 497, 591, 604, 988, 1017, 1142
TspGWI ACGGA 2 cut(s) 179, 945
TspRI CASTG 2 cut(s) 523, 663
VpaK11BI GGWCC 2 cut(s) 206, 276
XapI RAATTY 3 cut(s) 246, 665, 778
XbaI TCTAGA 1 cut(s) 1072
XceI RCATGY 2 cut(s) 43, 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XspI CTAG 6 cut(s) 371, 479, 489, 771, 1073, 1146
ZrmI AGTACT 1 cut(s) 388
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.