RLG00000025363

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0001367
Physical Location & Seq
Reverse (-)
45294106 .. 45296380
2275 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025363

Sequence Viewer

Length: 1128 bp
ATGGCCACCGTCTCCTCTCCTCCTTTTCAGTTTCCGGTCCCCAAACTCCACCCCGACCCGACCCGACTCCCCACCATCTTCAACCTCTCCTTCAACCAGGACCAAGCCTGCTTCGCCGTCGGCACCGACTTCGGCTTCCGCGTCTACAAGTGCGACCCCTTCGCCATCACCATCCGCCGCGACTTCTTCACAGGCGGCGGCTTCGGATTGGTCCGGATACTTAACACCTCCCAAATCTTCGCCATAGTCGGAGGCGGACCGGACCCGGTCTCCCCGGCCAACAGGGTCATGATCTGGGACGACCTCAAGTCCCTCCGCATCCACGACATGTCGTTTCGGTCTGAGGTCAGGGGGCTCAAGCTCCGGAGCGATCGACTCGTCGTCGTTTTGAACCAGAAGATCATCGTCCACAGCTTCCCTAATTCGAAGCCTCTGCACGAGATACTGACCACGGAGAACCCTAAGGGGCTGTGCGAGGTGTCGCAGAGTTCGGGTCCGATGGTGCTGGCGTGTCCGGGGCTCCATAAGGGTCAAATTCGGGTCGAGAATTACGCATTGAAGCGGACCAAGTTCATAATGGCCCACGATTCCAGTCTCGCCTGCTTTGGGCTCACTCAGGACGGCCGGTATTTGGCTACGGCGAGCAGCAAAGGGACTCTAGTTAGAGTTTTCAGTACTTCAGATGGTACATTACTCCAAGAGGTGAGGAGAGGAGCAGACAGAGCAGAGATTTACAATCTTGCTTTCTCTTGTACTGGCCAATGGCTAGCTGTGTCTAGTGACAAGGGAACTGTGCATGTTTTCAGTGTAAAGGTCGATTCAGGATCCATGGACAAGGATAGATCATGCGCTACATCAGAAGGCGGTGTTCCTAATCCATCAGGCATTTCATCTCTGTCTTTCATCAAAGGTGTGCTGCCAAGGTATTTCAGCTCAGAATGGTCGGTGGCCAAATTTCACTTGCAGGAAGGTCTGCAGCATATTGTTGCCTTTGGCAAGCGGAAGAACACCATCATGATTGTTGGCATGGATGGAAGCTTCTATCGATGCCAATTTGACCCTGTGAATGGAGGAGAAATGACGCAGCTTGAATATCATAACTTCGTGAAGCCAGAAGAAACTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

376

Amino Acids

41.42

Weight (kDa)

8.96

Isoelectric Point (pI)

46.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PROPPIN PF21032 29 - 364 2.6e-28 PROPPIN
Beta-prop_THOC3 PF25174 94 - 271 7e-12 THOC3 beta-propeller domain
Beta-prop_EML_2 PF23414 177 - 273 4.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 194 - 272 3.3e-07 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 144
AccII CGCG 2 cut(s) 141, 180
AccIII TCCGGA 2 cut(s) 213, 363
AclWI GGATC 2 cut(s) 819, 832
AcoI YGGCCR 5 cut(s) 3, 276, 622, 757, 948
AcsI RAATTY 2 cut(s) 534, 953
AcuI CTGAAG 1 cut(s) 663
AfaI GTAC 3 cut(s) 676, 688, 754
AfiI CCNNNNNNNGG 3 cut(s) 606, 631, 1067
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 5 cut(s) 82, 94, 391, 559, 1091
AhdI GACNNNNNGTC 2 cut(s) 307, 380
AjnI CCWGG 1 cut(s) 96
AluBI AGCT 6 cut(s) 361, 414, 770, 933, 1038, 1087
AluI AGCT 6 cut(s) 361, 414, 770, 933, 1038, 1087
Alw26I GTCTC 3 cut(s) 16, 274, 599
AlwI GGATC 2 cut(s) 819, 832
Aor13HI TCCGGA 2 cut(s) 213, 363
AoxI GGCC 6 cut(s) 3, 276, 579, 622, 757, 948
ApeKI GCWGC 4 cut(s) 645, 916, 976, 1084
ApoI RAATTY 2 cut(s) 534, 953
AspLEI GCGC 1 cut(s) 851
AspS9I GGNCC 8 cut(s) 37, 100, 211, 257, 262, 494, 564, 580
AsuC2I CCSGG 3 cut(s) 266, 275, 516
AsuHPI GGTGA 2 cut(s) 160, 715
AsuII TTCGAA 1 cut(s) 425
AsuNHI GCTAGC 1 cut(s) 766
AvaII GGWCC 7 cut(s) 37, 100, 211, 257, 262, 494, 564
AxyI CCTNAGG 1 cut(s) 462
BalI TGGCCA 3 cut(s) 5, 759, 950
BamHI GGATCC 1 cut(s) 824
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 3 cut(s) 357, 522, 612
BauI CACGAG 1 cut(s) 437
BbvI GCAGC 4 cut(s) 657, 903, 988, 1096
BccI CCATC 8 cut(s) 83, 173, 179, 493, 677, 886, 1019, 1025
BceAI ACGGC 3 cut(s) 101, 637, 654
BcgI CGANNNNNNTGC 4 cut(s) 112, 146, 415, 449
BciT130I CCWGG 1 cut(s) 98
BciVI GTATCC 1 cut(s) 210
BcnI CCSGG 3 cut(s) 266, 275, 516
BcoDI GTCTC 3 cut(s) 16, 274, 599
BfaI CTAG 3 cut(s) 659, 767, 777
BfmI CTRYAG 1 cut(s) 974
BfuI GTATCC 1 cut(s) 210
BisI GCNGC 7 cut(s) 178, 196, 199, 646, 917, 977, 1085
BlsI GCNGC 7 cut(s) 179, 197, 200, 647, 918, 978, 1086
BmcAI AGTACT 1 cut(s) 676
Bme1390I CCNGG 4 cut(s) 98, 266, 275, 516
Bme18I GGWCC 7 cut(s) 37, 100, 211, 257, 262, 494, 564
BmeRI GACNNNNNGTC 2 cut(s) 307, 380
BmgT120I GGNCC 8 cut(s) 37, 100, 211, 257, 262, 494, 564, 580
BmiI GGNNCC 6 cut(s) 39, 124, 264, 495, 521, 826
BmrFI CCNGG 4 cut(s) 98, 266, 275, 516
BmsI GCATC 2 cut(s) 327, 1037
BmtI GCTAGC 1 cut(s) 770
Bpu14I TTCGAA 1 cut(s) 425
BpuEI CTTGAG 2 cut(s) 290, 341
BpuMI CCSGG 3 cut(s) 266, 275, 516
Bsa29I ATCGAT 1 cut(s) 1045
BsaI GGTCTC 1 cut(s) 274
BsaJI CCNNGG 5 cut(s) 273, 450, 515, 828, 920
BsaWI WCCGGW 4 cut(s) 34, 213, 259, 363
BsaXI ACNNNNNCTCC 2 cut(s) 254, 284
Bsc4I CCNNNNNNNGG 3 cut(s) 606, 631, 1067
Bse118I RCCGGY 1 cut(s) 624
Bse1I ACTGG 2 cut(s) 591, 760
Bse21I CCTNAGG 1 cut(s) 462
BseAI TCCGGA 2 cut(s) 213, 363
BseBI CCWGG 1 cut(s) 98
BseCI ATCGAT 1 cut(s) 1045
BseDI CCNNGG 5 cut(s) 273, 450, 515, 828, 920
BseGI GGATG 3 cut(s) 171, 318, 1036
BseLI CCNNNNNNNGG 3 cut(s) 606, 631, 1067
BseMII CTCAG 3 cut(s) 333, 629, 948
BseNI ACTGG 2 cut(s) 591, 760
BseRI GAGGAG 5 cut(s) 4, 9, 721, 726, 1086
BseX3I CGGCCG 1 cut(s) 622
BseXI GCAGC 4 cut(s) 657, 903, 988, 1096
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 2 cut(s) 141, 180
Bsh1285I CGRYCG 2 cut(s) 373, 625
BshFI GGCC 6 cut(s) 5, 278, 581, 624, 759, 950
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 1045
BsiEI CGRYCG 2 cut(s) 373, 625
BsiSI CCGG 8 cut(s) 35, 214, 260, 266, 275, 364, 515, 625
BslFI GGGAC 4 cut(s) 23, 295, 311, 667
BslI CCNNNNNNNGG 3 cut(s) 606, 631, 1067
BsmAI GTCTC 3 cut(s) 16, 274, 599
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 4 cut(s) 23, 295, 311, 667
BsnI GGCC 6 cut(s) 5, 278, 581, 624, 759, 950
Bso31I GGTCTC 1 cut(s) 274
Bsp119I TTCGAA 1 cut(s) 425
Bsp1286I GDGCHC 3 cut(s) 357, 522, 612
Bsp13I TCCGGA 2 cut(s) 213, 363
Bsp143I GATC 5 cut(s) 291, 370, 399, 824, 842
Bsp19I CCATGG 1 cut(s) 828
BspANI GGCC 6 cut(s) 5, 278, 581, 624, 759, 950
BspCNI CTCAG 3 cut(s) 334, 628, 947
BspDI ATCGAT 1 cut(s) 1045
BspEI TCCGGA 2 cut(s) 213, 363
BspFNI CGCG 2 cut(s) 141, 180
BspHI TCATGA 2 cut(s) 288, 1014
BspLI GGNNCC 6 cut(s) 39, 124, 264, 495, 521, 826
BspMAI CTGCAG 1 cut(s) 978
BspOI GCTAGC 1 cut(s) 770
BspPI GGATC 2 cut(s) 819, 832
BspT104I TTCGAA 1 cut(s) 425
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 274
BsrFI RCCGGY 1 cut(s) 624
BsrI ACTGG 2 cut(s) 591, 760
BssAI RCCGGY 1 cut(s) 624
BssECI CCNNGG 5 cut(s) 273, 450, 515, 828, 920
BssMI GATC 5 cut(s) 291, 370, 399, 824, 842
BssSI CACGAG 1 cut(s) 437
BssT1I CCWWGG 2 cut(s) 828, 920
Bst2BI CACGAG 1 cut(s) 437
Bst2UI CCWGG 1 cut(s) 98
Bst4CI ACNGT 2 cut(s) 10, 793
BstBI TTCGAA 1 cut(s) 425
BstC8I GCNNGC 6 cut(s) 109, 507, 601, 643, 768, 998
BstDEI CTNAG 4 cut(s) 342, 462, 615, 934
BstDSI CCRYGG 2 cut(s) 450, 828
BstF5I GGATG 3 cut(s) 171, 318, 1036
BstFNI CGCG 2 cut(s) 141, 180
BstHHI GCGC 1 cut(s) 851
BstKTI GATC 5 cut(s) 294, 373, 402, 827, 845
BstMAI GTCTC 3 cut(s) 16, 274, 599
BstMBI GATC 5 cut(s) 291, 370, 399, 824, 842
BstMCI CGRYCG 2 cut(s) 373, 625
BstMWI GCNNNNNNNGC 2 cut(s) 113, 722
BstNI CCWGG 1 cut(s) 98
BstNSI RCATGY 2 cut(s) 331, 800
BstSCI CCNGG 4 cut(s) 96, 264, 273, 514
BstSFI CTRYAG 1 cut(s) 974
BstUI CGCG 2 cut(s) 141, 180
BstV1I GCAGC 4 cut(s) 657, 903, 988, 1096
BstX2I RGATCY 1 cut(s) 824
BstYI RGATCY 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 622
Bsu15I ATCGAT 1 cut(s) 1045
Bsu36I CCTNAGG 1 cut(s) 462
BsuI GTATCC 1 cut(s) 210
BsuRI GGCC 6 cut(s) 5, 278, 581, 624, 759, 950
BsuTUI ATCGAT 1 cut(s) 1045
BtgI CCRYGG 2 cut(s) 450, 828
BtsCI GGATG 3 cut(s) 171, 318, 1036
BtsIMutI CAGTG 1 cut(s) 811
Cac8I GCNNGC 6 cut(s) 109, 507, 601, 643, 768, 998
CciI TCATGA 2 cut(s) 288, 1014
CfoI GCGC 1 cut(s) 851
Cfr10I RCCGGY 1 cut(s) 624
Cfr13I GGNCC 8 cut(s) 37, 100, 211, 257, 262, 494, 564, 580
ClaI ATCGAT 1 cut(s) 1045
CpoI CGGWCCG 1 cut(s) 257
CseI GACGC 2 cut(s) 130, 1090
Csp6I GTAC 3 cut(s) 675, 687, 753
CspI CGGWCCG 1 cut(s) 257
CviAII CATG 7 cut(s) 289, 328, 797, 829, 846, 1015, 1027
CviQI GTAC 3 cut(s) 675, 687, 753
DdeI CTNAG 4 cut(s) 342, 462, 615, 934
DpnI GATC 5 cut(s) 293, 372, 401, 826, 844
DpnII GATC 5 cut(s) 291, 370, 399, 824, 842
DriI GACNNNNNGTC 2 cut(s) 307, 380
EaeI YGGCCR 5 cut(s) 3, 276, 622, 757, 948
EagI CGGCCG 1 cut(s) 622
Eam1105I GACNNNNNGTC 2 cut(s) 307, 380
EciI GGCGGA 2 cut(s) 164, 270
EclXI CGGCCG 1 cut(s) 622
Eco130I CCWWGG 2 cut(s) 828, 920
Eco24I GRGCYC 3 cut(s) 357, 522, 612
Eco31I GGTCTC 1 cut(s) 274
Eco47I GGWCC 7 cut(s) 37, 100, 211, 257, 262, 494, 564
Eco52I CGGCCG 1 cut(s) 622
Eco57I CTGAAG 1 cut(s) 663
Eco81I CCTNAGG 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 828, 920
EcoT38I GRGCYC 3 cut(s) 357, 522, 612
ErhI CCWWGG 2 cut(s) 828, 920
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 7 cut(s) 292, 331, 800, 832, 849, 1018, 1030
FaqI GGGAC 4 cut(s) 23, 295, 311, 667
FatI CATG 7 cut(s) 288, 327, 796, 828, 845, 1014, 1026
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 7 cut(s) 178, 196, 199, 646, 917, 977, 1085
FokI GGATG 3 cut(s) 158, 305, 1043
FriOI GRGCYC 3 cut(s) 357, 522, 612
Fsp4HI GCNGC 7 cut(s) 178, 196, 199, 646, 917, 977, 1085
FspBI CTAG 3 cut(s) 659, 767, 777
GlaI GCGC 1 cut(s) 850
GluI GCNGC 7 cut(s) 178, 196, 199, 646, 917, 977, 1085
HaeIII GGCC 6 cut(s) 5, 278, 581, 624, 759, 950
HapII CCGG 8 cut(s) 35, 214, 260, 266, 275, 364, 515, 625
HgaI GACGC 2 cut(s) 130, 1090
HhaI GCGC 1 cut(s) 851
Hin1II CATG 7 cut(s) 292, 331, 800, 832, 849, 1018, 1030
Hin6I GCGC 1 cut(s) 849
HinP1I GCGC 1 cut(s) 849
HindIII AAGCTT 1 cut(s) 1036
HinfI GANTC 5 cut(s) 66, 375, 587, 655, 818
HpaII CCGG 8 cut(s) 35, 214, 260, 266, 275, 364, 515, 625
HphI GGTGA 2 cut(s) 160, 715
Hpy166II GTNNAC 2 cut(s) 145, 409
Hpy188I TCNGA 7 cut(s) 206, 251, 343, 498, 682, 859, 937
Hpy188III TCNNGA 8 cut(s) 214, 289, 364, 544, 617, 822, 1015, 1105
Hpy8I GTNNAC 2 cut(s) 145, 409
Hpy99I CGWCG 3 cut(s) 122, 383, 386
HpyAV CCTTC 4 cut(s) 100, 169, 854, 962
HpyCH4III ACNGT 2 cut(s) 10, 793
HpyCH4V TGCA 4 cut(s) 436, 796, 964, 976
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 722
HpyF3I CTNAG 4 cut(s) 342, 462, 615, 934
Hsp92II CATG 7 cut(s) 292, 331, 800, 832, 849, 1018, 1030
HspAI GCGC 1 cut(s) 849
Kpn2I TCCGGA 2 cut(s) 213, 363
Kzo9I GATC 5 cut(s) 291, 370, 399, 824, 842
LmnI GCTCC 4 cut(s) 366, 366, 525, 713
Lsp1109I GCAGC 4 cut(s) 657, 903, 988, 1096
LweI GCATC 2 cut(s) 327, 1037
MaeI CTAG 3 cut(s) 659, 767, 777
MaeIII GTNAC 1 cut(s) 779
MalI GATC 5 cut(s) 293, 372, 401, 826, 844
MboI GATC 5 cut(s) 291, 370, 399, 824, 842
MboII GAAGA 6 cut(s) 70, 178, 229, 409, 1015, 1127
MflI RGATCY 1 cut(s) 824
MhlI GDGCHC 3 cut(s) 357, 522, 612
MlsI TGGCCA 3 cut(s) 5, 759, 950
MluCI AATT 5 cut(s) 421, 534, 547, 953, 1052
MluNI TGGCCA 3 cut(s) 5, 759, 950
MlyI GAGTC 3 cut(s) 60, 369, 649
MmeI TCCRAC 1 cut(s) 229
Mox20I TGGCCA 3 cut(s) 5, 759, 950
MroI TCCGGA 2 cut(s) 213, 363
MscI TGGCCA 3 cut(s) 5, 759, 950
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 1 cut(s) 1013
Msp20I TGGCCA 3 cut(s) 5, 759, 950
MspI CCGG 8 cut(s) 35, 214, 260, 266, 275, 364, 515, 625
MspR9I CCNGG 4 cut(s) 98, 266, 275, 516
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 2 cut(s) 141, 180
MwoI GCNNNNNNNGC 2 cut(s) 113, 722
NciI CCSGG 3 cut(s) 266, 275, 516
NcoI CCATGG 1 cut(s) 828
NdeII GATC 5 cut(s) 291, 370, 399, 824, 842
NheI GCTAGC 1 cut(s) 766
NlaIII CATG 7 cut(s) 292, 331, 800, 832, 849, 1018, 1030
NlaIV GGNNCC 6 cut(s) 39, 124, 264, 495, 521, 826
NmuCI GTSAC 1 cut(s) 779
NspI RCATGY 2 cut(s) 331, 800
NspV TTCGAA 1 cut(s) 425
PagI TCATGA 2 cut(s) 288, 1014
PciI ACATGT 1 cut(s) 327
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PfeI GAWTC 2 cut(s) 587, 818
PflFI GACNNNGTC 1 cut(s) 266
PkrI GCNGC 7 cut(s) 179, 197, 200, 647, 918, 978, 1086
Ple19I CGATCG 1 cut(s) 373
PleI GAGTC 3 cut(s) 60, 369, 649
PpsI GAGTC 3 cut(s) 60, 369, 649
PscI ACATGT 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 96
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 6 cut(s) 39, 124, 264, 495, 521, 826
PspPI GGNCC 8 cut(s) 37, 100, 211, 257, 262, 494, 564, 580
PstI CTGCAG 1 cut(s) 978
PsuI RGATCY 1 cut(s) 824
PsyI GACNNNGTC 1 cut(s) 266
PvuI CGATCG 1 cut(s) 373
RsaI GTAC 3 cut(s) 676, 688, 754
RsaNI GTAC 3 cut(s) 675, 687, 753
RseI CAYNNNNRTG 1 cut(s) 1013
Rsr2I CGGWCCG 1 cut(s) 257
RsrII CGGWCCG 1 cut(s) 257
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 7 cut(s) 178, 196, 199, 646, 917, 977, 1085
Sau3AI GATC 5 cut(s) 291, 370, 399, 824, 842
Sau96I GGNCC 8 cut(s) 37, 100, 211, 257, 262, 494, 564, 580
ScaI AGTACT 1 cut(s) 676
SchI GAGTC 3 cut(s) 60, 369, 649
ScrFI CCNGG 4 cut(s) 98, 266, 275, 516
SduI GDGCHC 3 cut(s) 357, 522, 612
SfaNI GCATC 2 cut(s) 327, 1037
SfcI CTRYAG 1 cut(s) 974
SfuI TTCGAA 1 cut(s) 425
SinI GGWCC 7 cut(s) 37, 100, 211, 257, 262, 494, 564
SmiMI CAYNNNNRTG 1 cut(s) 1013
SmlI CTYRAG 2 cut(s) 305, 356
SmoI CTYRAG 2 cut(s) 305, 356
Sse9I AATT 5 cut(s) 421, 534, 547, 953, 1052
SspMI CTAG 3 cut(s) 659, 767, 777
StyD4I CCNGG 4 cut(s) 96, 264, 273, 514
StyI CCWWGG 2 cut(s) 828, 920
TaaI ACNGT 2 cut(s) 10, 793
TaqI TCGA 5 cut(s) 373, 425, 543, 816, 1045
TaqII GACCGA 1 cut(s) 327
TasI AATT 5 cut(s) 421, 534, 547, 953, 1052
TatI WGTACW 2 cut(s) 674, 752
TauI GCSGC 3 cut(s) 180, 198, 201
TfiI GAWTC 2 cut(s) 587, 818
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 811
TseFI GTSAC 1 cut(s) 779
TseI GCWGC 4 cut(s) 645, 916, 976, 1084
Tsp45I GTSAC 1 cut(s) 779
TspDTI ATGAA 3 cut(s) 562, 879, 892
TspGWI ACGGA 1 cut(s) 467
TspRI CASTG 1 cut(s) 811
Tth111I GACNNNGTC 1 cut(s) 266
VpaK11BI GGWCC 7 cut(s) 37, 100, 211, 257, 262, 494, 564
XapI RAATTY 2 cut(s) 534, 953
XceI RCATGY 2 cut(s) 331, 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmiI GTMKAC 1 cut(s) 144
XspI CTAG 3 cut(s) 659, 767, 777
ZrmI AGTACT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.