Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000477

Physical Location & Seq

Genomic Coordinates Forward (+)

10813183 .. 10817070

Gene Structure 3888 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G19960.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2472 bp

ATGGCGGAAAGAGTCGAAGCATCATCTGTACCAGAAGGAGAAAATACAATAGAAGAAAGAGAAGTAGGAGCTATGTGGGAATTAGAGCAGAAACTTGATCAACCCATGGATGAAGAAGCTAATAAGCTCAACAACATGTATAGAGAAAAGGGATTATCAATGTTGATGCTTCTAAGGCTCTCTTTTCAAAGTCTAGGGATAGTTTATGGAGATTTAGGGACGTCTCCATTGTATGTGTTTTACAATACATTCCCTGACGGAATTGATGATAGTGAAGATGTAATCGGAGCTCTTTCTTTGATCATTTACTCTCTTTTGCTTATTCCTCTCATCAAGTATGTGTTCATTGTCTGCAAAGCTAATGATAATGGTCAAGGTGGGACTTTAGCTATATATTCGTTGCTTTGTAGACATGCTAAAGTGAAACTAATCCCGAATCAGCACCGTAGTGATGAGGATCTCACGACTTATAGTCGAACTGTATCCGCTGAAGGGTCTTTTGCTGCTAAAACCAAGAAATGGTTGGAGGGTAAAGAATGGAGGAAGAGAGCTCTTCTTGTCGTTGTTCTTCTCGGTACTTGTATGATGATCGGTGATGGTATCTTAACTCCAGCTATCTCCGTTCTTTCAGCAACTGGTGGGATCAAAGTAAACAATCCAAAAATGAGCGGCGATATCGTCGTGCTTGTGGCAATTGTCATTTTGATAGGATTGTTCAGTATGCAACACTATGGTACAGACAAAGTCGGATGGCTCTTTGCACCTATCGTGCTTATTTGGTTCCTCTTCATTGGAGCCACTGGGATGTACAACATCTGCAAGTATGACACAAGCGTTTTAAAAGCGTTTTCGCCTACATATATATACTTGTACTTCAAAAGACGAGGTCGAGATGGTTGGATATCGCTCGGTGGCATTCTTCTCAGCATAACAGGCACGGAGGCACTATACGCAGACATTGCCTATTTCCCCTTACTAGCGATACAGCTCGCCTTTACGTTTTTTGTGTTCCCTTGTCTTCTTCTAGCATACTGTGGACAAGCTGCTTATCTTGTGATTCATAAGGAACATTATCAAGACGCGTTCTATGCATCTATCCCGGATAGTGTCTATTGGCCAATGTTTATAGTCGCGACAGGAGCTGCTATTGTTGGGAGCCAAGCTACGATCTCCGGGACTTATTCGATCGTCAAGCAGGCCGTGGCTCATGGATGTTTTCCTCGAGTAAAAATTGTTCATACTTCCAAGAAGTTCCTCGGTCAGATCTATTGTCCTGATATTAACTGGATACTCATGCTCGGCTGCATCGCGGTTACTGCTAGTTTCAAAAAGCAGAGCCAAATAGGAAATGCATACGGTAAGATGACAACAACTTCAAAATACAAGAAAAATTATTTTAGTCAATGGACTGCGGTTGTTCTCGTGATGCTTGTGACCACATTACTAATGGTGCTGATAATGCTACTCGTGTGGCATTGCCACTGGATCCTTGTCCTTATATTCACCTTTCTCTCCTTCTTTGTGGAGTTATCGTACTTCTCAGCCGTGATCTTTAAAATCGATGAAGGAGGATGGGTGCCGCTCATCATAGCAGCGATATCTCTTTTAGTAATGTCAGTCTGGCATTACGCAACAGTCAAGAAATATGAGTTTGAAATGCACAGTAAAGTGTCCATGAGTTGGATACTCGGCCTCGGTCCCAGCCTCGGGCTTGTCCGTGTCCCGGGGATCGGGTTAGTCTACACGGAATTAGCAAGTGGTGTTCCTCACATTTTCTCTCATTTCATCACCAACCTCCCCGCGATTCATTCGGTTGTAGTCTTTGTCTGCGTCAAGTACCTTCCGGTTTACACCGTCCCTGAAGAAGAGAGGTTTCTTGTGAAGAGAATCGGACCAAAAACATTCCGGATGTTCCGATGTGTAGCCAGATATGGTTACAAAGATCTACACAAGAAAGACGATGATTTCGAGAACAAACTGCTGACTAAACTCTCCTCATTCATTCGAATCGAAACTATGATGGAGCCAACTTCAAACTCAAGCACCTACAGCAGCACGTATTCCGTCAACCATACACAAGATTCCACCGTAGACTTGATCCACAACAACAACAACCACAACCACAACAACAACATGGATATGTTCTCATCGATGGTGGACTACACGGTATCAACTTTAGACACGATAGTTTCCGCTGAATCACTGCATAACACGGTGAGTTTCAGTCAGGACAACACGGTAGAGGAGGAGGAGACGGATGAGTTGGAGTTTCTAAAGACTTGTAAAGAGTCAGGTGTTGTTCATATAATGGGGAACACCGTGGTGAAAGCAAGAACTGGATCATGGCTTCCAAAAAAGATTGCGATTGATTATGTTTATGCATTTCTTGCTAAGATTTGTAGGGCCAATAGTGTTATCTTACATGTTCCTCATGAAACCCTTTTAAACGTTGGACAAGTCTTTTATGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

823

Amino Acids

92.37

Weight (kDa)

6.9

Isoelectric Point (pI)

34.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	61 - 207	7.8e-36	K+ potassium transporter integral membrane domain
K_trans	PF02705	233 - 572	6.5e-115	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	585 - 830	2.3e-21	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca	FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica	MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica	Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis	pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis	RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata	RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora	Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa	Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha	Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana	Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	224
AccB1I	GGYRCC	1 cut(s)	1576
AccB7I	CCANNNNNTGG	2 cut(s)	519, 1680
AccBSI	CCGCTC	2 cut(s)	669, 1582
AccI	GTMKAC	3 cut(s)	409, 1740, 2091
AccII	CGCG	4 cut(s)	1082, 1133, 1310, 1802
AccIII	TCCGGA	1 cut(s)	1905
AciI	CCGC	8 cut(s)	5, 486, 669, 1310, 1412, 1580, 1800, 2193
AclI	AACGTT	1 cut(s)	2448
AclWI	GGATC	7 cut(s)	465, 650, 1480, 1493, 1736, 2092, 2347
AcoI	YGGCCR	1 cut(s)	1115
AcuI	CTGAAG	2 cut(s)	510, 1881
AcyI	GRCGYC	1 cut(s)	221
AfaI	GTAC	7 cut(s)	30, 577, 736, 809, 872, 1535, 1838
AfiI	CCNNNNNNNGG	7 cut(s)	492, 519, 1680, 1706, 1707, 1723, 1730
AflIII	ACRYGT	3 cut(s)	135, 1080, 2422
AgsI	TTSAA	6 cut(s)	188, 877, 1327, 1377, 1655, 2034
AhdI	GACNNNNNGTC	1 cut(s)	471
AleI	CACNNNNGTG	2 cut(s)	447, 2321
Alw21I	GWGCWC	2 cut(s)	292, 553
Alw26I	GTCTC	2 cut(s)	228, 2246
AlwI	GGATC	7 cut(s)	465, 650, 1480, 1493, 1736, 2092, 2347
AlwNI	CAGNNNCTG	2 cut(s)	635, 1142
Ama87I	CYCGRG	3 cut(s)	1221, 1706, 1723
Aor13HI	TCCGGA	1 cut(s)	1905
AoxI	GGCC	4 cut(s)	1115, 1197, 1690, 2403
ApeKI	GCWGC	6 cut(s)	503, 1043, 1142, 1302, 1592, 2052
ArsI	GACNNNNNNTTYG	2 cut(s)	1949, 1981
AspS9I	GGNCC	3 cut(s)	1697, 1892, 2403
AsuC2I	CCSGG	4 cut(s)	1100, 1174, 1724, 1725
AsuHPI	GGTGA	5 cut(s)	605, 1495, 1780, 2227, 2335
AsuII	TTCGAA	1 cut(s)	2005
AvaI	CYCGRG	3 cut(s)	1221, 1706, 1723
AvaII	GGWCC	2 cut(s)	1697, 1892
BalI	TGGCCA	1 cut(s)	1117
BamHI	GGATCC	1 cut(s)	1485
BanI	GGYRCC	1 cut(s)	1576
BanII	GRGCYC	2 cut(s)	292, 553
BauI	CACGAG	2 cut(s)	1421, 1466
BbsI	GAAGAC	1 cut(s)	1010
Bbv12I	GWGCWC	2 cut(s)	292, 553
BbvI	GCAGC	6 cut(s)	490, 1030, 1129, 1289, 1604, 2064
BccI	CCATC	6 cut(s)	590, 744, 887, 1566, 2014, 2146
BceAI	ACGGC	2 cut(s)	1184, 1529
BciVI	GTATCC	3 cut(s)	493, 1281, 1677
BclI	TGATCA	2 cut(s)	97, 300
BcnI	CCSGG	4 cut(s)	1100, 1174, 1724, 1725
BcoDI	GTCTC	2 cut(s)	228, 2246
BfaI	CTAG	4 cut(s)	194, 977, 1025, 1320
BfmI	CTRYAG	1 cut(s)	2047
BfuI	GTATCC	3 cut(s)	493, 1281, 1677
BglII	AGATCT	2 cut(s)	1263, 1942
BisI	GCNGC	8 cut(s)	504, 670, 1044, 1143, 1303, 1580, 1593, 2053
BlsI	GCNGC	8 cut(s)	505, 671, 1045, 1144, 1304, 1581, 1594, 2054
Bme1390I	CCNGG	4 cut(s)	1100, 1174, 1724, 1725
Bme18I	GGWCC	2 cut(s)	1697, 1892
BmeRI	GACNNNNNGTC	1 cut(s)	471
BmeT110I	CYCGRG	3 cut(s)	1221, 1706, 1723
BmgT120I	GGNCC	3 cut(s)	1697, 1892, 2403
BmiI	GGNNCC	7 cut(s)	782, 796, 1157, 1487, 1578, 1699, 2025
BmrFI	CCNGG	4 cut(s)	1100, 1174, 1724, 1725
BmrI	ACTGGG	1 cut(s)	810
BmsI	GCATC	5 cut(s)	29, 156, 1100, 1314, 1416
BmuI	ACTGGG	1 cut(s)	810
BpiI	GAAGAC	1 cut(s)	1010
BpmI	CTGGAG	1 cut(s)	594
Bpu14I	TTCGAA	1 cut(s)	2005
BpuEI	CTTGAG	1 cut(s)	2023
BpuMI	CCSGG	4 cut(s)	1100, 1174, 1724, 1725
Bsa29I	ATCGAT	2 cut(s)	1560, 2150
BsaAI	YACGTR	1 cut(s)	2058
BsaBI	GATNNNNATC	1 cut(s)	456
BsaHI	GRCGYC	1 cut(s)	221
BsaJI	CCNNGG	8 cut(s)	105, 1200, 1255, 1693, 1705, 1723, 1724, 2319
BsaWI	WCCGGW	2 cut(s)	1843, 1905
BsaXI	ACNNNNNCTCC	4 cut(s)	1517, 1547, 1560, 1590
Bsc4I	CCNNNNNNNGG	7 cut(s)	492, 519, 1680, 1706, 1707, 1723, 1730
Bse1I	ACTGG	5 cut(s)	640, 805, 1289, 1487, 2341
Bse3DI	GCAATG	2 cut(s)	957, 1474
Bse8I	GATNNNNATC	1 cut(s)	456
BseAI	TCCGGA	1 cut(s)	1905
BseCI	ATCGAT	2 cut(s)	1560, 2150
BseDI	CCNNGG	8 cut(s)	105, 1200, 1255, 1693, 1705, 1723, 1724, 2319
BseGI	GGATG	7 cut(s)	115, 755, 810, 1217, 1577, 1914, 2263
BseJI	GATNNNNATC	1 cut(s)	456
BseLI	CCNNNNNNNGG	7 cut(s)	492, 519, 1680, 1706, 1707, 1723, 1730
BseMI	GCAATG	2 cut(s)	957, 1474
BseMII	CTCAG	2 cut(s)	937, 1554
BseNI	ACTGG	5 cut(s)	640, 805, 1289, 1487, 2341
BseRI	GAGGAG	4 cut(s)	1984, 2258, 2261, 2264
BseXI	GCAGC	6 cut(s)	490, 1030, 1129, 1289, 1604, 2064
BseYI	CCCAGC	1 cut(s)	1700
Bsh1236I	CGCG	4 cut(s)	1082, 1133, 1310, 1802
Bsh1285I	CGRYCG	1 cut(s)	1188
BshFI	GGCC	4 cut(s)	1117, 1199, 1692, 2405
BshNI	GGYRCC	1 cut(s)	1576
BshVI	ATCGAT	2 cut(s)	1560, 2150
BsiEI	CGRYCG	1 cut(s)	1188
BsiHKAI	GWGCWC	2 cut(s)	292, 553
BsiHKCI	CYCGRG	3 cut(s)	1221, 1706, 1723
BsiSI	CCGG	5 cut(s)	1100, 1173, 1724, 1844, 1906
BslFI	GGGAC	6 cut(s)	232, 394, 1189, 1683, 1706, 1841
BslI	CCNNNNNNNGG	7 cut(s)	492, 519, 1680, 1706, 1707, 1723, 1730
BsmAI	GTCTC	2 cut(s)	228, 2246
BsmBI	CGTCTC	2 cut(s)	228, 2246
BsmFI	GGGAC	6 cut(s)	232, 394, 1189, 1683, 1706, 1841
BsmI	GAATGC	1 cut(s)	915
BsnI	GGCC	4 cut(s)	1117, 1199, 1692, 2405
BsoBI	CYCGRG	3 cut(s)	1221, 1706, 1723
Bsp119I	TTCGAA	1 cut(s)	2005
Bsp1286I	GDGCHC	2 cut(s)	292, 553
Bsp13I	TCCGGA	1 cut(s)	1905
Bsp1407I	TGTACA	1 cut(s)	807
Bsp19I	CCATGG	1 cut(s)	105
Bsp68I	TCGCGA	1 cut(s)	1133
BspACI	CCGC	8 cut(s)	5, 486, 669, 1310, 1412, 1580, 1800, 2193
BspANI	GGCC	4 cut(s)	1117, 1199, 1692, 2405
BspCNI	CTCAG	2 cut(s)	936, 1553
BspDI	ATCGAT	2 cut(s)	1560, 2150
BspEI	TCCGGA	1 cut(s)	1905
BspFNI	CGCG	4 cut(s)	1082, 1133, 1310, 1802
BspHI	TCATGA	1 cut(s)	2431
BspLI	GGNNCC	7 cut(s)	782, 796, 1157, 1487, 1578, 1699, 2025
BspPI	GGATC	7 cut(s)	465, 650, 1480, 1493, 1736, 2092, 2347
BspQI	GCTCTTC	1 cut(s)	558
BspT104I	TTCGAA	1 cut(s)	2005
BspT107I	GGYRCC	1 cut(s)	1576
BsrBI	CCGCTC	2 cut(s)	669, 1582
BsrDI	GCAATG	2 cut(s)	957, 1474
BsrGI	TGTACA	1 cut(s)	807
BsrI	ACTGG	5 cut(s)	640, 805, 1289, 1487, 2341
BssECI	CCNNGG	8 cut(s)	105, 1200, 1255, 1693, 1705, 1723, 1724, 2319
BssNI	GRCGYC	1 cut(s)	221
BssSI	CACGAG	2 cut(s)	1421, 1466
BssT1I	CCWWGG	1 cut(s)	105
Bst2BI	CACGAG	2 cut(s)	1421, 1466
Bst6I	CTCTTC	5 cut(s)	539, 558, 791, 1860, 1877
BstACI	GRCGYC	1 cut(s)	221
BstAPI	GCANNNNNTGC	2 cut(s)	959, 2387
BstAUI	TGTACA	1 cut(s)	807
BstBAI	YACGTR	1 cut(s)	2058
BstBI	TTCGAA	1 cut(s)	2005
BstC8I	GCNNGC	2 cut(s)	990, 1197
BstDEI	CTNAG	4 cut(s)	173, 923, 1540, 2391
BstDSI	CCRYGG	3 cut(s)	105, 1200, 2319
BstF5I	GGATG	7 cut(s)	115, 755, 810, 1217, 1577, 1914, 2263
BstFNI	CGCG	4 cut(s)	1082, 1133, 1310, 1802
BstMAI	GTCTC	2 cut(s)	228, 2246
BstMCI	CGRYCG	1 cut(s)	1188
BstNSI	RCATGY	3 cut(s)	139, 416, 2426
BstSCI	CCNGG	4 cut(s)	1098, 1172, 1722, 1723
BstSFI	CTRYAG	1 cut(s)	2047
BstUI	CGCG	4 cut(s)	1082, 1133, 1310, 1802
BstV1I	GCAGC	6 cut(s)	490, 1030, 1129, 1289, 1604, 2064
BstV2I	GAAGAC	1 cut(s)	1010
BstX2I	RGATCY	4 cut(s)	457, 1263, 1485, 1942
BstYI	RGATCY	4 cut(s)	457, 1263, 1485, 1942
Bsu15I	ATCGAT	2 cut(s)	1560, 2150
BsuI	GTATCC	3 cut(s)	493, 1281, 1677
BsuRI	GGCC	4 cut(s)	1117, 1199, 1692, 2405
BsuTUI	ATCGAT	2 cut(s)	1560, 2150
BtgI	CCRYGG	3 cut(s)	105, 1200, 2319
BtgZI	GCGATG	1 cut(s)	1291
BtsCI	GGATG	7 cut(s)	115, 755, 810, 1217, 1577, 1914, 2263
BtsI	GCAGTG	1 cut(s)	2201
BtsIMutI	CAGTG	3 cut(s)	798, 1480, 2201
BtuMI	TCGCGA	1 cut(s)	1133
Cac8I	GCNNGC	2 cut(s)	990, 1197
CaiI	CAGNNNCTG	2 cut(s)	635, 1142
CciI	TCATGA	1 cut(s)	2431
Cfr13I	GGNCC	3 cut(s)	1697, 1892, 2403
Cfr9I	CCCGGG	1 cut(s)	1723
ClaI	ATCGAT	2 cut(s)	1560, 2150
CseI	GACGC	2 cut(s)	1088, 1819
Csp6I	GTAC	7 cut(s)	29, 576, 735, 808, 871, 1534, 1837
CviQI	GTAC	7 cut(s)	29, 576, 735, 808, 871, 1534, 1837
DdeI	CTNAG	4 cut(s)	173, 923, 1540, 2391
DraI	TTTAAA	3 cut(s)	840, 1555, 2445
DriI	GACNNNNNGTC	1 cut(s)	471
EaeI	YGGCCR	1 cut(s)	1115
Eam1104I	CTCTTC	5 cut(s)	539, 558, 791, 1860, 1877
Eam1105I	GACNNNNNGTC	1 cut(s)	471
EarI	CTCTTC	5 cut(s)	539, 558, 791, 1860, 1877
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	2 cut(s)	290, 551
Eco130I	CCWWGG	1 cut(s)	105
Eco24I	GRGCYC	2 cut(s)	292, 553
Eco32I	GATATC	3 cut(s)	676, 903, 1599
Eco47I	GGWCC	2 cut(s)	1697, 1892
Eco53kI	GAGCTC	2 cut(s)	290, 551
Eco57I	CTGAAG	2 cut(s)	510, 1881
Eco88I	CYCGRG	3 cut(s)	1221, 1706, 1723
EcoICRI	GAGCTC	2 cut(s)	290, 551
EcoRV	GATATC	3 cut(s)	676, 903, 1599
EcoT14I	CCWWGG	1 cut(s)	105
EcoT22I	ATGCAT	3 cut(s)	1093, 1354, 2383
EcoT38I	GRGCYC	2 cut(s)	292, 553
ErhI	CCWWGG	1 cut(s)	105
Esp3I	CGTCTC	2 cut(s)	228, 2246
FalI	AAGNNNNNCTT	2 cut(s)	166, 198
FaqI	GGGAC	6 cut(s)	232, 394, 1189, 1683, 1706, 1841
FauI	CCCGC	1 cut(s)	1807
FbaI	TGATCA	2 cut(s)	97, 300
FblI	GTMKAC	3 cut(s)	409, 1740, 2091
Fnu4HI	GCNGC	8 cut(s)	504, 670, 1044, 1143, 1303, 1580, 1593, 2053
FokI	GGATG	7 cut(s)	122, 762, 817, 1224, 1584, 1921, 2270
FriOI	GRGCYC	2 cut(s)	292, 553
Fsp4HI	GCNGC	8 cut(s)	504, 670, 1044, 1143, 1303, 1580, 1593, 2053
FspBI	CTAG	4 cut(s)	194, 977, 1025, 1320
GluI	GCNGC	8 cut(s)	504, 670, 1044, 1143, 1303, 1580, 1593, 2053
GsaI	CCCAGC	1 cut(s)	1704
GsuI	CTGGAG	1 cut(s)	594
HaeIII	GGCC	4 cut(s)	1117, 1199, 1692, 2405
HapII	CCGG	5 cut(s)	1100, 1173, 1724, 1844, 1906
HgaI	GACGC	2 cut(s)	1088, 1819
Hin1I	GRCGYC	1 cut(s)	221
HincII	GTYRAC	1 cut(s)	2068
HindII	GTYRAC	1 cut(s)	2068
HinfI	GANTC	9 cut(s)	12, 436, 1057, 1804, 1887, 2007, 2081, 2198, 2288
HpaII	CCGG	5 cut(s)	1100, 1173, 1724, 1844, 1906
HphI	GGTGA	5 cut(s)	605, 1495, 1780, 2227, 2335
Hpy166II	GTNNAC	8 cut(s)	410, 652, 1037, 1741, 1849, 2068, 2092, 2158
Hpy188I	TCNGA	5 cut(s)	287, 749, 1263, 1892, 1916
Hpy8I	GTNNAC	8 cut(s)	410, 652, 1037, 1741, 1849, 2068, 2092, 2158
Hpy99I	CGWCG	1 cut(s)	683
HpyAV	CCTTC	5 cut(s)	29, 485, 1525, 1559, 1850
HpyCH4IV	ACGT	4 cut(s)	221, 998, 2057, 2448
HpyF3I	CTNAG	4 cut(s)	173, 923, 1540, 2391
HpySE526I	ACGT	4 cut(s)	221, 998, 2057, 2448
Hsp92I	GRCGYC	1 cut(s)	221
Kpn2I	TCCGGA	1 cut(s)	1905
Ksp22I	TGATCA	2 cut(s)	97, 300
LguI	GCTCTTC	1 cut(s)	558
LmnI	GCTCC	6 cut(s)	68, 287, 794, 1139, 1155, 2023
Lsp1109I	GCAGC	6 cut(s)	490, 1030, 1129, 1289, 1604, 2064
LweI	GCATC	5 cut(s)	29, 156, 1100, 1314, 1416
MaeI	CTAG	4 cut(s)	194, 977, 1025, 1320
MaeII	ACGT	4 cut(s)	221, 998, 2057, 2448
MaeIII	GTNAC	3 cut(s)	1312, 1432, 1934
MbiI	CCGCTC	2 cut(s)	669, 1582
MfeI	CAATTG	1 cut(s)	693
MflI	RGATCY	4 cut(s)	457, 1263, 1485, 1942
MhlI	GDGCHC	2 cut(s)	292, 553
MlsI	TGGCCA	1 cut(s)	1117
MluCI	AATT	6 cut(s)	80, 261, 693, 1230, 1390, 1748
MluI	ACGCGT	1 cut(s)	1080
MluNI	TGGCCA	1 cut(s)	1117
MlyI	GAGTC	2 cut(s)	21, 2297
MmeI	TCCRAC	6 cut(s)	504, 727, 878, 1661, 2244, 2431
Mox20I	TGGCCA	1 cut(s)	1117
Mph1103I	ATGCAT	3 cut(s)	1093, 1354, 2383
MroI	TCCGGA	1 cut(s)	1905
MscI	TGGCCA	1 cut(s)	1117
MseI	TTAA	5 cut(s)	605, 839, 1281, 1554, 2444
MslI	CAYNNNNRTG	4 cut(s)	447, 803, 2138, 2321
Msp20I	TGGCCA	1 cut(s)	1117
MspA1I	CMGCKG	2 cut(s)	488, 2195
MspI	CCGG	5 cut(s)	1100, 1173, 1724, 1844, 1906
MspR9I	CCNGG	4 cut(s)	1100, 1174, 1724, 1725
MunI	CAATTG	1 cut(s)	693
Mva1269I	GAATGC	1 cut(s)	915
MvnI	CGCG	4 cut(s)	1082, 1133, 1310, 1802
NciI	CCSGG	4 cut(s)	1100, 1174, 1724, 1725
NcoI	CCATGG	1 cut(s)	105
NlaIV	GGNNCC	7 cut(s)	782, 796, 1157, 1487, 1578, 1699, 2025
NmeAIII	GCCGAG	2 cut(s)	1278, 1668
NmuCI	GTSAC	1 cut(s)	1432
NruI	TCGCGA	1 cut(s)	1133
NsiI	ATGCAT	3 cut(s)	1093, 1354, 2383
NspI	RCATGY	3 cut(s)	139, 416, 2426
NspV	TTCGAA	1 cut(s)	2005
OliI	CACNNNNGTG	2 cut(s)	447, 2321
PaeR7I	CTCGAG	1 cut(s)	1221
PagI	TCATGA	1 cut(s)	2431
PciI	ACATGT	2 cut(s)	135, 2422
PciSI	GCTCTTC	1 cut(s)	558
PcsI	WCGNNNNNNNCGW	1 cut(s)	1965
PctI	GAATGC	1 cut(s)	915
PfeI	GAWTC	7 cut(s)	436, 1057, 1804, 1887, 2007, 2081, 2198
PflFI	GACNNNGTC	2 cut(s)	743, 885
PflMI	CCANNNNNTGG	2 cut(s)	519, 1680
PfoI	TCCNGGA	2 cut(s)	1098, 1172
PkrI	GCNGC	8 cut(s)	505, 671, 1045, 1144, 1304, 1581, 1594, 2054
Ple19I	CGATCG	1 cut(s)	1188
PleI	GAGTC	2 cut(s)	20, 2296
PpsI	GAGTC	2 cut(s)	20, 2296
Ppu21I	YACGTR	1 cut(s)	2058
PscI	ACATGT	2 cut(s)	135, 2422
Psp124BI	GAGCTC	2 cut(s)	292, 553
Psp1406I	AACGTT	1 cut(s)	2448
PspFI	CCCAGC	1 cut(s)	1700
PspN4I	GGNNCC	7 cut(s)	782, 796, 1157, 1487, 1578, 1699, 2025
PspPI	GGNCC	3 cut(s)	1697, 1892, 2403
PspXI	VCTCGAGB	1 cut(s)	1221
PsrI	GAACNNNNNNTAC	2 cut(s)	1218, 1250
PstNI	CAGNNNCTG	2 cut(s)	635, 1142
PsuI	RGATCY	4 cut(s)	457, 1263, 1485, 1942
PsyI	GACNNNGTC	2 cut(s)	743, 885
PvuI	CGATCG	1 cut(s)	1188
RruI	TCGCGA	1 cut(s)	1133
RsaI	GTAC	7 cut(s)	30, 577, 736, 809, 872, 1535, 1838
RsaNI	GTAC	7 cut(s)	29, 576, 735, 808, 871, 1534, 1837
RseI	CAYNNNNRTG	4 cut(s)	447, 803, 2138, 2321
SacI	GAGCTC	2 cut(s)	292, 553
SapI	GCTCTTC	1 cut(s)	558
SaqAI	TTAA	5 cut(s)	605, 839, 1281, 1554, 2444
SatI	GCNGC	8 cut(s)	504, 670, 1044, 1143, 1303, 1580, 1593, 2053
Sau96I	GGNCC	3 cut(s)	1697, 1892, 2403
SchI	GAGTC	2 cut(s)	21, 2297
ScrFI	CCNGG	4 cut(s)	1100, 1174, 1724, 1725
SduI	GDGCHC	2 cut(s)	292, 553
SfaNI	GCATC	5 cut(s)	29, 156, 1100, 1314, 1416
SfcI	CTRYAG	1 cut(s)	2047
Sfr274I	CTCGAG	1 cut(s)	1221
SfuI	TTCGAA	1 cut(s)	2005
SinI	GGWCC	2 cut(s)	1697, 1892
SlaI	CTCGAG	1 cut(s)	1221
SmaI	CCCGGG	1 cut(s)	1725
SmiMI	CAYNNNNRTG	4 cut(s)	447, 803, 2138, 2321
SmlI	CTYRAG	2 cut(s)	1221, 2038
SmoI	CTYRAG	2 cut(s)	1221, 2038
Sse9I	AATT	6 cut(s)	80, 261, 693, 1230, 1390, 1748
SsiI	CCGC	8 cut(s)	5, 486, 669, 1310, 1412, 1580, 1800, 2193
SspMI	CTAG	4 cut(s)	194, 977, 1025, 1320
SstI	GAGCTC	2 cut(s)	292, 553
StyD4I	CCNGG	4 cut(s)	1098, 1172, 1722, 1723
StyI	CCWWGG	1 cut(s)	105
TaiI	ACGT	4 cut(s)	224, 1001, 2060, 2451
TaqII	GACCGA	2 cut(s)	1247, 1685
TasI	AATT	6 cut(s)	80, 261, 693, 1230, 1390, 1748
TatI	WGTACW	2 cut(s)	807, 870
TauI	GCSGC	2 cut(s)	672, 1582
TfiI	GAWTC	7 cut(s)	436, 1057, 1804, 1887, 2007, 2081, 2198
Tru1I	TTAA	5 cut(s)	605, 839, 1281, 1554, 2444
Tru9I	TTAA	5 cut(s)	605, 839, 1281, 1554, 2444
TscAI	CASTG	3 cut(s)	805, 1487, 2208
TseFI	GTSAC	1 cut(s)	1432
TseI	GCWGC	6 cut(s)	503, 1043, 1142, 1302, 1592, 2052
Tsp45I	GTSAC	1 cut(s)	1432
TspGWI	ACGGA	7 cut(s)	273, 610, 953, 1706, 1760, 2053, 2270
TspMI	CCCGGG	1 cut(s)	1723
TspRI	CASTG	3 cut(s)	805, 1487, 2208
Tth111I	GACNNNGTC	2 cut(s)	743, 885
Van91I	CCANNNNNTGG	2 cut(s)	519, 1680
VpaK11BI	GGWCC	2 cut(s)	1697, 1892
XceI	RCATGY	3 cut(s)	139, 416, 2426
XcmI	CCANNNNNNNNNTGG	2 cut(s)	520, 1444
XhoI	CTCGAG	1 cut(s)	1221
XmaI	CCCGGG	1 cut(s)	1723
XmiI	GTMKAC	3 cut(s)	409, 1740, 2091
XspI	CTAG	4 cut(s)	194, 977, 1025, 1320
ZraI	GACGTC	1 cut(s)	222
Zsp2I	ATGCAT	3 cut(s)	1093, 1354, 2383

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.