Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0000477

Physical Location & Seq

Genomic Coordinates Reverse (-)

32732453 .. 32739862

Gene Structure 7410 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1230400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2418 bp

ATGGAAGCTGTAGAATCATTTGAAATTTCAAAAGCTTCAAAAGTGGAGGTTGATGGAGACAGTGATAACACCAACAAAGGCAGTATGTGGGTTTTGGATCAGAAGCTTGACCAACCCATGGATGAGGAGGCTGGCAGGCTTAGAACCATGTACAGGGAAAAGAAATTCTCCTCGCTGTTGCTTATGCGGCTTGCATTCCAAAGTCTTGGAGTGGTTTATGGAGATTTGGGTACTTCTCCCTTGTATGTTTTCTACAATACCTTTCCCCATGGAATTAACGATCCGGAGGATGTGGTTGGAGCTCTTTCGTTGATTATATACTCTCTTACTCTAGTCCCGCTCCTCAAGTATGTATTTGTTGTTTGTCGGGCGAATGACAATGGTCAAGGTGGCACATTTGCTCTTTATTCATTGCTTTGTCGACATGCAAATGTAAAAACTATTCCCAACCAACACCGGACTGATAAGGACCTAACGACATATAGTCGCTCTACTTTCCATGAACAATCATATGCTGCAAAAACCAAGAAGTGGTTGGAGAGGTATGCATCAAGGAAGAATTCCCTTCTTATTCTGGTCCTCGTAGGCACTTGTATGGTGATTGGAGATGGGATTCTTACTCCAGCTATATCTGTTTTATCAGCTGCTGGTGGGATCAAAGTAAGCAGCCCTGGCATGAAAAATGATTATGTCATACTCGTTGCTGTTGTAATATTAGTAGGTCTGTTTAGCATGCAGCACTATGGTACTGATAAAGTTGGTTGGCTGTTTGCTCCCATTGTGCTGCTTTGGTTTCTCTTAATTGGAGGTATTGGCATATTCAACATCTGGAAACATGATAGTACTGTTTTGAGAGCTTTTTCCCCTGTGTATATATATCGGTATTTTAAAAGGAACGGGAGAGAGGGTTGGATTTCCCTTGGGGGTATTATGCTTAGCATAACAGGGACAGAAGCACTCTTTGCCGACCTTGCCCATTTTCCAGTTTCAGCTGTACAGATTGCGTTTACTACAGTCGTGTTTCCTTGCCTTCTTTTAGCCTATTGCGGACAGGCTGCCTTCCTTGTGAAAAATCATTCAGACGAAATCCTGCTTCAAGCATTTTATCGTTCTATTCCAGAAAAAATATACTGGCCAGTCTTCATTGTTGCGACCTTAGCTGCTATAGTTGCGAGTCAAGCGACAATATCAGCAACTTTTTCAATAATCAAGCAGGCTCTTGCACTTGGCTGTTTTCCTAGAGTCAAAGTTGTACATACATCAAAGAAGTTCCTCGGCCAAATATATATTCCAGATATTAATTGGATCCTTATGATTCTTTGCATTGCTGTGACTGCGGGGTTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCAGTTGTAGTAGTCATGCTGGCAACTACATTGCTTATGACTCTAATCATGATATTAGTGTGGCGCTGCCACTGGATTCTTGTCGCGATATTCACTGGCTTGTCACTGCTTGTGGAATGCACTTACTTTTCTGCTGTACTCTTCAAGGTTGATCAAGGTGGATGGGTACCTCTTGTGATTGCAGCTGCTTTTCTTCTCATCATGTATGTTTGGCATTATGGTACGTTGAAACGTTATGAGTTTGAGATGCACAGTAAGGTTTCAATGGCATGGCTTCTTGGGCTTGGCCCCAGTTTAGGACTGGTCCGGGTCCCTGGGATAGGACTTGTGTACACCGAGCTTGCAAGCGGGGTGCCCCACATCTTTTCCCACTTCATCACCAACCTGCCCGCAATCCACCAGGTTGTTGTCCTTGTCTGTGTGAAGTACCTCCCTGTATACACCGTGCCAGAAGAAGAAAGATTCCTCGTCAAGCGGATTGGCCCCAAGAACTTCCACATGTTTCGTTGTGTTGCAAGATACGGCTATAAAGACCTCCACAAGAAGGATGATGACTTTGAGAAGAAGCTCTTTGAAAACCTTTTCTTGTTTGTCCGGCTCGATTCCATGATGGAAGGGTGTTCAGACTCAGACGAGTACAGCATCTATGGCCAGCAAACTGAGAGGTCAAGGGACGGCCTCTTAATTAATAATGGCAACACGATCACATCTACAGTAGACCTCAGTATCTCATCAGTGGACTCAATTGTGCCGGCTAAATCTCCATCGCACGTGAACATTACCGTGAGTTCATCCGGGCAGATGAGCAGCCAGAATGATATTGATGAGCTAGAGTTTTTGAATGAGTGTCGAGACTCCGGGGTGGTGCATATACTTGGGAATACAGTAGTGAGAGCAAGGAGGGATTCCAGGTTTTACAAAAAGATAGCTGTTGATTACATTTATGCATTTCTCAGGAAAATTTGCAGGGAGAATAGTGTGATCTTCAATGTTCCTCATGAGAGTCTACTGAATGTTGGACAGATTTTCTACGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

806

Amino Acids

90.05

Weight (kDa)

8.54

Isoelectric Point (pI)

37.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	64 - 551	3.1e-163	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	565 - 802	3.2e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca	FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica	MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica	Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis	pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis	RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata	RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora	Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa	Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha	Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana	Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1773
Acc65I	GGTACC	1 cut(s)	1546
AccB1I	GGYRCC	2 cut(s)	1546, 1732
AccB7I	CCANNNNNTGG	2 cut(s)	118, 531
AccBSI	CCGCTC	1 cut(s)	340
AccI	GTMKAC	4 cut(s)	421, 1818, 2097, 2386
AccII	CGCG	1 cut(s)	1466
AccIII	TCCGGA	1 cut(s)	283
AciI	CCGC	7 cut(s)	187, 338, 1047, 1337, 1728, 1770, 1855
AclI	AACGTT	1 cut(s)	1612
AclWI	GGATC	5 cut(s)	105, 275, 662, 1300, 1313
AcoI	YGGCCR	3 cut(s)	1133, 1276, 2029
AcsI	RAATTY	4 cut(s)	24, 164, 559, 2340
AcvI	CACGTG	1 cut(s)	2152
AfiI	CCNNNNNNNGG	6 cut(s)	118, 153, 531, 1676, 1700, 1924
AflIII	ACRYGT	2 cut(s)	1878, 2412
AhdI	GACNNNNNGTC	1 cut(s)	483
AjnI	CCWGG	4 cut(s)	670, 1693, 1779, 2288
AjuI	GAANNNNNNNTTGG	2 cut(s)	1272, 1304
Alw21I	GWGCWC	1 cut(s)	304
Alw26I	GTCTC	2 cut(s)	51, 2226
AlwI	GGATC	5 cut(s)	105, 275, 662, 1300, 1313
AlwNI	CAGNNNCTG	1 cut(s)	647
Aor13HI	TCCGGA	1 cut(s)	283
AoxI	GGCC	6 cut(s)	1133, 1276, 1666, 1861, 2029, 2056
ApoI	RAATTY	4 cut(s)	24, 164, 559, 2340
AseI	ATTAAT	2 cut(s)	1299, 2067
Asp718I	GGTACC	1 cut(s)	1546
AspLEI	GCGC	1 cut(s)	1446
AspS9I	GGNCC	6 cut(s)	469, 577, 1667, 1684, 1690, 1862
AsuC2I	CCSGG	3 cut(s)	1688, 2176, 2239
AsuHPI	GGTGA	2 cut(s)	610, 1750
AvaII	GGWCC	4 cut(s)	469, 577, 1684, 1690
BaeGI	GKGCMC	1 cut(s)	1737
BaeI	ACNNNNGTAYC	2 cut(s)	2089, 2122
BalI	TGGCCA	2 cut(s)	1135, 2031
BamHI	GGATCC	1 cut(s)	1305
BanI	GGYRCC	2 cut(s)	1546, 1732
BanII	GRGCYC	1 cut(s)	304
BbrPI	CACGTG	1 cut(s)	2152
BbsI	GAAGAC	1 cut(s)	1132
Bbv12I	GWGCWC	1 cut(s)	304
BccI	CCATC	5 cut(s)	47, 602, 1536, 1984, 2152
BceAI	ACGGC	3 cut(s)	1395, 1918, 2071
BciT130I	CCWGG	4 cut(s)	672, 1695, 1781, 2290
BclI	TGATCA	1 cut(s)	1531
BcnI	CCSGG	3 cut(s)	1688, 2176, 2239
BcoDI	GTCTC	2 cut(s)	51, 2226
BfaI	CTAG	3 cut(s)	332, 1239, 2210
BfmI	CTRYAG	4 cut(s)	9, 1011, 1164, 2091
BfoI	RGCGCY	1 cut(s)	1447
BfuAI	ACCTGC	1 cut(s)	1773
BlpI	GCTNAGC	1 cut(s)	935
BmcAI	AGTACT	1 cut(s)	844
Bme1390I	CCNGG	7 cut(s)	672, 1688, 1695, 1781, 2176, 2239, 2290
Bme18I	GGWCC	4 cut(s)	469, 577, 1684, 1690
BmeRI	GACNNNNNGTC	1 cut(s)	483
BmgT120I	GGNCC	6 cut(s)	469, 577, 1667, 1684, 1690, 1862
BmiI	GGNNCC	7 cut(s)	1307, 1548, 1669, 1691, 1692, 1734, 1864
BmrFI	CCNGG	7 cut(s)	672, 1688, 1695, 1781, 2176, 2239, 2290
BmrI	ACTGGG	1 cut(s)	1665
BmsI	GCATC	3 cut(s)	557, 1617, 2031
BmuI	ACTGGG	1 cut(s)	1665
BoxI	GACNNNNGTC	1 cut(s)	381
BpiI	GAAGAC	1 cut(s)	1132
BpmI	CTGGAG	1 cut(s)	606
Bpu10I	CCTNAGC	1 cut(s)	1156
Bpu1102I	GCTNAGC	1 cut(s)	935
BpuEI	CTTGAG	1 cut(s)	329
BpuMI	CCSGG	3 cut(s)	1688, 2176, 2239
BsaAI	YACGTR	2 cut(s)	2152, 2413
BsaJI	CCNNGG	8 cut(s)	117, 268, 670, 919, 1273, 1693, 1694, 2238
BsaWI	WCCGGW	2 cut(s)	283, 456
BsaXI	ACNNNNNCTCC	2 cut(s)	892, 922
Bsc4I	CCNNNNNNNGG	6 cut(s)	118, 153, 531, 1676, 1700, 1924
Bse118I	RCCGGY	1 cut(s)	2131
Bse1I	ACTGG	7 cut(s)	983, 1136, 1136, 1457, 1480, 1671, 1686
Bse3DI	GCAATG	3 cut(s)	410, 1323, 1409
BseAI	TCCGGA	1 cut(s)	283
BseBI	CCWGG	4 cut(s)	672, 1695, 1781, 2290
BseDI	CCNNGG	8 cut(s)	117, 268, 670, 919, 1273, 1693, 1694, 2238
BseGI	GGATG	5 cut(s)	127, 295, 1547, 1933, 2171
BseLI	CCNNNNNNNGG	6 cut(s)	118, 153, 531, 1676, 1700, 1924
BseMI	GCAATG	3 cut(s)	410, 1323, 1409
BseMII	CTCAG	4 cut(s)	2022, 2031, 2116, 2347
BseNI	ACTGG	7 cut(s)	983, 1136, 1136, 1457, 1480, 1671, 1686
BseRI	GAGGAG	3 cut(s)	140, 160, 332
BseSI	GKGCMC	1 cut(s)	1737
Bsh1236I	CGCG	1 cut(s)	1466
BshFI	GGCC	6 cut(s)	1135, 1278, 1668, 1863, 2031, 2058
BshNI	GGYRCC	2 cut(s)	1546, 1732
BsiHKAI	GWGCWC	1 cut(s)	304
BsiSI	CCGG	7 cut(s)	284, 457, 1687, 1975, 2132, 2175, 2238
BslFI	GGGAC	5 cut(s)	320, 961, 1390, 1676, 2066
BslI	CCNNNNNNNGG	6 cut(s)	118, 153, 531, 1676, 1700, 1924
BsmAI	GTCTC	2 cut(s)	51, 2226
BsmFI	GGGAC	5 cut(s)	320, 961, 1390, 1676, 2066
BsmI	GAATGC	2 cut(s)	194, 1502
BsnI	GGCC	6 cut(s)	1135, 1278, 1668, 1863, 2031, 2058
Bsp1286I	GDGCHC	2 cut(s)	304, 1737
Bsp13I	TCCGGA	1 cut(s)	283
Bsp1407I	TGTACA	4 cut(s)	150, 994, 1252, 1710
Bsp143I	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
Bsp1720I	GCTNAGC	1 cut(s)	935
Bsp19I	CCATGG	2 cut(s)	117, 268
Bsp68I	TCGCGA	1 cut(s)	1466
BspACI	CCGC	7 cut(s)	187, 338, 1047, 1337, 1728, 1770, 1855
BspANI	GGCC	6 cut(s)	1135, 1278, 1668, 1863, 2031, 2058
BspCNI	CTCAG	4 cut(s)	2021, 2032, 2115, 2346
BspEI	TCCGGA	1 cut(s)	283
BspFNI	CGCG	1 cut(s)	1466
BspHI	TCATGA	2 cut(s)	1428, 2377
BspLI	GGNNCC	7 cut(s)	1307, 1548, 1669, 1691, 1692, 1734, 1864
BspMI	ACCTGC	1 cut(s)	1773
BspPI	GGATC	5 cut(s)	105, 275, 662, 1300, 1313
BspT107I	GGYRCC	2 cut(s)	1546, 1732
BsrBI	CCGCTC	1 cut(s)	340
BsrDI	GCAATG	3 cut(s)	410, 1323, 1409
BsrFI	RCCGGY	1 cut(s)	2131
BsrGI	TGTACA	4 cut(s)	150, 994, 1252, 1710
BsrI	ACTGG	7 cut(s)	983, 1136, 1136, 1457, 1480, 1671, 1686
BssAI	RCCGGY	1 cut(s)	2131
BssECI	CCNNGG	8 cut(s)	117, 268, 670, 919, 1273, 1693, 1694, 2238
BssMI	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
BssNAI	GTATAC	1 cut(s)	1819
BssT1I	CCWWGG	3 cut(s)	117, 268, 919
Bst1107I	GTATAC	1 cut(s)	1819
Bst2UI	CCWGG	4 cut(s)	672, 1695, 1781, 2290
Bst4CI	ACNGT	8 cut(s)	62, 847, 1015, 1634, 1825, 2095, 2164, 2266
Bst6I	CTCTTC	1 cut(s)	1526
BstAPI	GCANNNNNTGC	1 cut(s)	962
BstAUI	TGTACA	4 cut(s)	150, 994, 1252, 1710
BstBAI	YACGTR	2 cut(s)	2152, 2413
BstDEI	CTNAG	7 cut(s)	140, 935, 1156, 2008, 2040, 2102, 2333
BstDSI	CCRYGG	2 cut(s)	117, 268
BstENI	CCTNNNNNAGG	1 cut(s)	1698
BstF5I	GGATG	5 cut(s)	127, 295, 1547, 1933, 2171
BstFNI	CGCG	1 cut(s)	1466
BstH2I	RGCGCY	1 cut(s)	1447
BstHHI	GCGC	1 cut(s)	1446
BstKTI	GATC	7 cut(s)	100, 283, 657, 1308, 1534, 2085, 2364
BstMAI	GTCTC	2 cut(s)	51, 2226
BstMBI	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
BstNI	CCWGG	4 cut(s)	672, 1695, 1781, 2290
BstNSI	RCATGY	3 cut(s)	428, 736, 1882
BstPAI	GACNNNNGTC	1 cut(s)	381
BstSCI	CCNGG	7 cut(s)	670, 1686, 1693, 1779, 2174, 2237, 2288
BstSFI	CTRYAG	4 cut(s)	9, 1011, 1164, 2091
BstSLI	GKGCMC	1 cut(s)	1737
BstUI	CGCG	1 cut(s)	1466
BstV2I	GAAGAC	1 cut(s)	1132
BstX2I	RGATCY	1 cut(s)	1305
BstXI	CCANNNNNNTGG	1 cut(s)	206
BstYI	RGATCY	1 cut(s)	1305
BstZ17I	GTATAC	1 cut(s)	1819
BsuRI	GGCC	6 cut(s)	1135, 1278, 1668, 1863, 2031, 2058
BtgI	CCRYGG	2 cut(s)	117, 268
BtgZI	GCGATG	1 cut(s)	2130
BtsCI	GGATG	5 cut(s)	127, 295, 1547, 1933, 2171
BtsI	GCAGTG	1 cut(s)	1484
BtsIMutI	CAGTG	5 cut(s)	67, 1450, 1473, 1484, 2121
BtuMI	TCGCGA	1 cut(s)	1466
BveI	ACCTGC	1 cut(s)	1773
CaiI	CAGNNNCTG	1 cut(s)	647
CciI	TCATGA	2 cut(s)	1428, 2377
CfoI	GCGC	1 cut(s)	1446
Cfr10I	RCCGGY	1 cut(s)	2131
Cfr13I	GGNCC	6 cut(s)	469, 577, 1667, 1684, 1690, 1862
CsiI	ACCWGGT	1 cut(s)	1779
DdeI	CTNAG	7 cut(s)	140, 935, 1156, 2008, 2040, 2102, 2333
DpnI	GATC	7 cut(s)	99, 282, 656, 1307, 1533, 2084, 2363
DpnII	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
DraI	TTTAAA	1 cut(s)	889
DriI	GACNNNNNGTC	1 cut(s)	483
EaeI	YGGCCR	3 cut(s)	1133, 1276, 2029
Eam1104I	CTCTTC	1 cut(s)	1526
Eam1105I	GACNNNNNGTC	1 cut(s)	483
EarI	CTCTTC	1 cut(s)	1526
Ecl136II	GAGCTC	1 cut(s)	302
Eco130I	CCWWGG	3 cut(s)	117, 268, 919
Eco24I	GRGCYC	1 cut(s)	304
Eco47I	GGWCC	4 cut(s)	469, 577, 1684, 1690
Eco53kI	GAGCTC	1 cut(s)	302
Eco72I	CACGTG	1 cut(s)	2152
EcoICRI	GAGCTC	1 cut(s)	302
EcoNI	CCTNNNNNAGG	1 cut(s)	1698
EcoO109I	RGGNCCY	2 cut(s)	469, 1690
EcoRI	GAATTC	1 cut(s)	559
EcoRII	CCWGG	4 cut(s)	670, 1693, 1779, 2288
EcoT14I	CCWWGG	3 cut(s)	117, 268, 919
EcoT22I	ATGCAT	2 cut(s)	550, 2329
EcoT38I	GRGCYC	1 cut(s)	304
ErhI	CCWWGG	3 cut(s)	117, 268, 919
FalI	AAGNNNNNCTT	2 cut(s)	1934, 1966
FaqI	GGGAC	5 cut(s)	320, 961, 1390, 1676, 2066
FauI	CCCGC	4 cut(s)	345, 1330, 1721, 1777
FauNDI	CATATG	1 cut(s)	511
FbaI	TGATCA	1 cut(s)	1531
FblI	GTMKAC	4 cut(s)	421, 1818, 2097, 2386
FokI	GGATG	5 cut(s)	134, 302, 1554, 1940, 2158
FriOI	GRGCYC	1 cut(s)	304
FspBI	CTAG	3 cut(s)	332, 1239, 2210
GlaI	GCGC	1 cut(s)	1445
GsuI	CTGGAG	1 cut(s)	606
HaeII	RGCGCY	1 cut(s)	1447
HaeIII	GGCC	6 cut(s)	1135, 1278, 1668, 1863, 2031, 2058
HapII	CCGG	7 cut(s)	284, 457, 1687, 1975, 2132, 2175, 2238
HhaI	GCGC	1 cut(s)	1446
Hin6I	GCGC	1 cut(s)	1444
HinP1I	GCGC	1 cut(s)	1444
HincII	GTYRAC	1 cut(s)	422
HindII	GTYRAC	1 cut(s)	422
HindIII	AAGCTT	2 cut(s)	33, 104
HpaII	CCGG	7 cut(s)	284, 457, 1687, 1975, 2132, 2175, 2238
HphI	GGTGA	2 cut(s)	610, 1750
Hpy166II	GTNNAC	9 cut(s)	422, 1008, 1711, 1713, 1819, 2098, 2119, 2155, 2387
Hpy188I	TCNGA	4 cut(s)	102, 1081, 2005, 2011
Hpy188III	TCNNGA	9 cut(s)	284, 829, 1118, 1292, 1429, 1465, 2231, 2335, 2378
Hpy8I	GTNNAC	9 cut(s)	422, 1008, 1711, 1713, 1819, 2098, 2119, 2155, 2387
HpyAV	CCTTC	5 cut(s)	575, 1040, 1069, 1918, 1988
HpyCH4III	ACNGT	8 cut(s)	62, 847, 1015, 1634, 1825, 2095, 2164, 2266
HpyCH4IV	ACGT	4 cut(s)	1604, 1612, 2151, 2412
HpyF3I	CTNAG	7 cut(s)	140, 935, 1156, 2008, 2040, 2102, 2333
HpySE526I	ACGT	4 cut(s)	1604, 1612, 2151, 2412
HspAI	GCGC	1 cut(s)	1444
KflI	GGGWCCC	1 cut(s)	1690
Kpn2I	TCCGGA	1 cut(s)	283
KpnI	GGTACC	1 cut(s)	1550
KroI	GCCGGC	1 cut(s)	2131
KroNI	GCCGGC	1 cut(s)	2133
Ksp22I	TGATCA	1 cut(s)	1531
Kzo9I	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
LmnI	GCTCC	3 cut(s)	299, 345, 778
LweI	GCATC	3 cut(s)	557, 1617, 2031
MabI	ACCWGGT	1 cut(s)	1779
MaeI	CTAG	3 cut(s)	332, 1239, 2210
MaeII	ACGT	4 cut(s)	1604, 1612, 2151, 2412
MaeIII	GTNAC	2 cut(s)	1330, 1482
MalI	GATC	7 cut(s)	99, 282, 656, 1307, 1533, 2084, 2363
MbiI	CCGCTC	1 cut(s)	340
MboI	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
MboII	GAAGA	8 cut(s)	568, 1132, 1513, 1565, 1844, 1847, 1954, 2356
MfeI	CAATTG	1 cut(s)	2124
MflI	RGATCY	1 cut(s)	1305
MhlI	GDGCHC	2 cut(s)	304, 1737
MlsI	TGGCCA	2 cut(s)	1135, 2031
MluNI	TGGCCA	2 cut(s)	1135, 2031
MlyI	GAGTC	7 cut(s)	1183, 1251, 1414, 2000, 2114, 2228, 2392
MmeI	TCCRAC	4 cut(s)	277, 516, 890, 2377
Mox20I	TGGCCA	2 cut(s)	1135, 2031
Mph1103I	ATGCAT	2 cut(s)	550, 2329
MroI	TCCGGA	1 cut(s)	283
MroNI	GCCGGC	1 cut(s)	2131
MscI	TGGCCA	2 cut(s)	1135, 2031
MseI	TTAA	6 cut(s)	276, 800, 888, 1299, 2063, 2067
MslI	CAYNNNNRTG	4 cut(s)	429, 593, 1328, 2162
Msp20I	TGGCCA	2 cut(s)	1135, 2031
MspA1I	CMGCKG	3 cut(s)	644, 992, 1565
MspI	CCGG	7 cut(s)	284, 457, 1687, 1975, 2132, 2175, 2238
MspR9I	CCNGG	7 cut(s)	672, 1688, 1695, 1781, 2176, 2239, 2290
MunI	CAATTG	1 cut(s)	2124
Mva1269I	GAATGC	2 cut(s)	194, 1502
MvaI	CCWGG	4 cut(s)	672, 1695, 1781, 2290
MvnI	CGCG	1 cut(s)	1466
NaeI	GCCGGC	1 cut(s)	2133
NciI	CCSGG	3 cut(s)	1688, 2176, 2239
NcoI	CCATGG	2 cut(s)	117, 268
NdeI	CATATG	1 cut(s)	511
NdeII	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
NgoMIV	GCCGGC	1 cut(s)	2131
NlaIV	GGNNCC	7 cut(s)	1307, 1548, 1669, 1691, 1692, 1734, 1864
NmeAIII	GCCGAG	1 cut(s)	1254
NmuCI	GTSAC	2 cut(s)	1330, 1482
NruI	TCGCGA	1 cut(s)	1466
NsiI	ATGCAT	2 cut(s)	550, 2329
NspI	RCATGY	3 cut(s)	428, 736, 1882
PacI	TTAATTAA	1 cut(s)	2067
PaeI	GCATGC	1 cut(s)	736
PagI	TCATGA	2 cut(s)	1428, 2377
PasI	CCCWGGG	1 cut(s)	1694
PciI	ACATGT	1 cut(s)	1878
PctI	GAATGC	2 cut(s)	194, 1502
PdiI	GCCGGC	1 cut(s)	2133
PfeI	GAWTC	7 cut(s)	14, 613, 1315, 1456, 1842, 1982, 2285
PflMI	CCANNNNNTGG	2 cut(s)	118, 531
PleI	GAGTC	7 cut(s)	1182, 1250, 1414, 2000, 2114, 2228, 2391
PmaCI	CACGTG	1 cut(s)	2152
PmlI	CACGTG	1 cut(s)	2152
PpsI	GAGTC	7 cut(s)	1182, 1250, 1414, 2000, 2114, 2228, 2391
Ppu21I	YACGTR	2 cut(s)	2152, 2413
PpuMI	RGGWCCY	2 cut(s)	469, 1690
PscI	ACATGT	1 cut(s)	1878
PshAI	GACNNNNGTC	1 cut(s)	381
PshBI	ATTAAT	2 cut(s)	1299, 2067
Psp124BI	GAGCTC	1 cut(s)	304
Psp1406I	AACGTT	1 cut(s)	1612
Psp5II	RGGWCCY	2 cut(s)	469, 1690
Psp6I	CCWGG	4 cut(s)	670, 1693, 1779, 2288
PspCI	CACGTG	1 cut(s)	2152
PspGI	CCWGG	4 cut(s)	670, 1693, 1779, 2288
PspN4I	GGNNCC	7 cut(s)	1307, 1548, 1669, 1691, 1692, 1734, 1864
PspPI	GGNCC	6 cut(s)	469, 577, 1667, 1684, 1690, 1862
PspPPI	RGGWCCY	2 cut(s)	469, 1690
PstNI	CAGNNNCTG	1 cut(s)	647
PsuI	RGATCY	1 cut(s)	1305
PvuII	CAGCTG	3 cut(s)	644, 992, 1565
RruI	TCGCGA	1 cut(s)	1466
RseI	CAYNNNNRTG	4 cut(s)	429, 593, 1328, 2162
SacI	GAGCTC	1 cut(s)	304
SalI	GTCGAC	1 cut(s)	420
SaqAI	TTAA	6 cut(s)	276, 800, 888, 1299, 2063, 2067
Sau3AI	GATC	7 cut(s)	97, 280, 654, 1305, 1531, 2082, 2361
Sau96I	GGNCC	6 cut(s)	469, 577, 1667, 1684, 1690, 1862
ScaI	AGTACT	1 cut(s)	844
SchI	GAGTC	7 cut(s)	1183, 1251, 1414, 2000, 2114, 2228, 2392
ScrFI	CCNGG	7 cut(s)	672, 1688, 1695, 1781, 2176, 2239, 2290
SduI	GDGCHC	2 cut(s)	304, 1737
SexAI	ACCWGGT	1 cut(s)	1779
SfaNI	GCATC	3 cut(s)	557, 1617, 2031
SfcI	CTRYAG	4 cut(s)	9, 1011, 1164, 2091
SinI	GGWCC	4 cut(s)	469, 577, 1684, 1690
SmiMI	CAYNNNNRTG	4 cut(s)	429, 593, 1328, 2162
SmlI	CTYRAG	1 cut(s)	344
SmoI	CTYRAG	1 cut(s)	344
SphI	GCATGC	1 cut(s)	736
SsiI	CCGC	7 cut(s)	187, 338, 1047, 1337, 1728, 1770, 1855
SspI	AATATT	1 cut(s)	714
SspMI	CTAG	3 cut(s)	332, 1239, 2210
SstI	GAGCTC	1 cut(s)	304
StyD4I	CCNGG	7 cut(s)	670, 1686, 1693, 1779, 2174, 2237, 2288
StyI	CCWWGG	3 cut(s)	117, 268, 919
TaaI	ACNGT	8 cut(s)	62, 847, 1015, 1634, 1825, 2095, 2164, 2266
TaiI	ACGT	4 cut(s)	1607, 1615, 2154, 2415
TaqI	TCGA	3 cut(s)	421, 1980, 2230
TatI	WGTACW	7 cut(s)	150, 842, 994, 1252, 1516, 1710, 2016
TauI	GCSGC	1 cut(s)	190
TfiI	GAWTC	7 cut(s)	14, 613, 1315, 1456, 1842, 1982, 2285
Tru1I	TTAA	6 cut(s)	276, 800, 888, 1299, 2063, 2067
Tru9I	TTAA	6 cut(s)	276, 800, 888, 1299, 2063, 2067
TscAI	CASTG	5 cut(s)	67, 1457, 1480, 1491, 2121
TseFI	GTSAC	2 cut(s)	1330, 1482
Tsp45I	GTSAC	2 cut(s)	1330, 1482
TspDTI	ATGAA	6 cut(s)	399, 516, 692, 1132, 1744, 2160
TspRI	CASTG	5 cut(s)	67, 1457, 1480, 1491, 2121
Van91I	CCANNNNNTGG	2 cut(s)	118, 531
VpaK11BI	GGWCC	4 cut(s)	469, 577, 1684, 1690
VspI	ATTAAT	2 cut(s)	1299, 2067
XagI	CCTNNNNNAGG	1 cut(s)	1698
XapI	RAATTY	4 cut(s)	24, 164, 559, 2340
XceI	RCATGY	3 cut(s)	428, 736, 1882
XcmI	CCANNNNNNNNNTGG	3 cut(s)	532, 1299, 1678
XmiI	GTMKAC	4 cut(s)	421, 1818, 2097, 2386
XspI	CTAG	3 cut(s)	332, 1239, 2210
ZrmI	AGTACT	1 cut(s)	844
Zsp2I	ATGCAT	2 cut(s)	550, 2329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.