RchiOBHm_Chr5g0020471

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000477
Physical Location & Seq
Forward (+)
14689679 .. 14695515
5837 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30056

Sequence Viewer

Length: 2361 bp
ATGGCTACTAATGAAGAAACTGGTGGCAAAGGTGGAGTGTGGGTTTTGGATCAGCAGTTTGATCAGCCTATGGATGAGGAGGCCAAGAGGCTTAGAAATATGTACCAAGAAAAGAAAATTTCAACAGCAATTCTTCTGCAGCTTGCATTTCAAAGCCTTGGTGTGGTCTATGGAGATTTAGGCACATCTCCTTTGTATGTCTTCTACAATACATTTCCCAGGGGAATTGACGATCCCGAGGATTTAATTGGAGCTTTGTCTGTCATTATATATTCCCTCACTCTTATTCCCCTTGTGAAGTATGTTTTTATCGTCTGTAGAGCAAATGACAATGGTCAAGGTGGAACATTTGCTCTTTATTCGTTACTCTGTCGACATGCCAAACTTAAAGCTATTCCTAACCAGGACAGAACAGATGAGGAGCTAACAACTTATAGCTCTTCTACATTTGGTGAGCAATCATTTGCTGCAAAAACCAAGAGATGGTTGGAGGGACATGCATTGATGAAGAGCACACTTCTTATCCTTGTCCTGATTGGATCTTGCATGGTGATTGGAGATGGGATTCTAACTCCAGCCATATCAGTTCTTTCAGCAGTTGGTGGGATCAATGTCCGTCACTCTCGGATTAGTAATGAGGTAGTTGTGCTTGTTGCTGTCGTCATACTGGTTCTTTTGTTTACCATGCAACACCACGGTACAGATAAAGTTGGTTGGCTTTTTGCACCAATTGTGCTCCTCTGGTTTCTCTTGATTGGAGGGATTGGCATGTTCAACATTTGGAAATTCGACAGGAACGTCTTAAAGGCTTTCTCGCCTTTGTATGTATATCGATTTTTTAAGAAGGGAGGAAAAGATGGGTGGACATCTCTGGGAGGTATAATGCTCAGCATAACAGGAACAGAAGCACTTTTTGCTGACTTGTCCCATTTCCCAGTCCCATCAATACAGATTGCTTTCACATGTGTTGTATTTCCTTCCCTTCTGTTAGCCTACTCTGGTCAAGCTGCCTACCTCATGAAACATTCTGATCATGTGTACGGTGCTTTTTACCATTCTATTCCAGACAGTATTTATTGGCCTGTGTTTGTTGTTGCTACTGCTGCTGCTGTTGTAGCGAGTCAGGCCACCATAACTGCAACTTTTGCATTAGTTAAGCAAGCCCTTGCACTTGGCTGTTTCCCAAGAGTCAAAGTCGTACATATGTCAAGGAAGTACCGTCACCAGATATATATTCCAGAAGTTAATTGGATTGTTATGGTTCTCTGCATTGCCGTGACAGCTGGGTTTAAAAATCAAAACCAAATTGGGAACGCCTCTGGGACGGCAGTTTGTATTGTTATGTTGGTGACCACATTGCTTATGATTTTAGTCATGATATTAGTGTGGCACTGCCATTGGGTTCTTGTCCTAATCTTCACAGGCTTATCATTTCTAATGGAGGGCACTTACTTTTCTGCTGTACTCCTCAAGGTCAATCAAGGAGGCTGGGTTCCTCTTGTGATATCAGCAGCTTTCTTTATTATTATGTATGTATGGCATTATGGTACAGTAAAGCGCTATGAGATTGAGGTTCACAGTAAGGTTTCAATGGCATGGATTCTTGGACTTGGTCCCAGTTTAGGACTGGTTCGGGTACCTGGTGTAGGCCTTGTGTACACTGAGCTAGCAAGTGGAGTTCCCCGCATCTTCTCTCACTTTATCACCAACCTACCCGCCATTCATTCTGTTGTTGTCTTTGTCTGTGTGAAGTACCTTCCTGTCTGTACAGTCCCAGAAGAAGAGCGGTTCCTTGTAAAGCGGATTGGACCCAAGAATTTTCACATGTTCCGTTGTGTTGCCAGATATGGGTATAAAGATCTCCATAGAAAAGATGATGATTTTGAGAAAAAGCTCTTTGACAGCCTTTTCATGTTTCTCCGGCTTGAGTCCCTGATGGAGGCATCATCAGACTCTGATGTATCTAGTTTACTTGACCAGCAGACTAAGCAATCAGGAGATGGCCTATTCAGTAATAACAGCGATCCTTACTCCATTGTAGACCAGTCAATCGCATCAGGGGACTCAATCGTGCCAGCTGATTCTTCATTGGGCGCAAACAATGCTGTGATATCCACCCATCAGGCAGGCAGCCCGGTGGAGATTGATGAGATAGAGTTCTTGAACAACTGTAAAGATGCAGGGGTGGTGCACATATTGGGAAATACTGTAGTAAGAGCCAGGAAGAACTCAAAATTTTGGAAGAAGATAGCTATTGATTATCTCTATGCATTTCTTAGGAAGATATGTAGGGAAAATAGTGTAATGTTTAACATTCCTCATGAGAGTCTCTTGAATGTTGGGCAGATATTCTATGTATAA

Protein Analysis

786

Amino Acids

87.29

Weight (kDa)

8.26

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 48 - 534 1.2e-159 K+ potassium transporter integral membrane domain
K_trans_C PF22776 547 - 783 2.2e-24 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 797
Acc65I GGTACC 1 cut(s) 1636
AccB1I GGYRCC 1 cut(s) 1636
AccB7I CCANNNNNTGG 1 cut(s) 483
AccBSI CCGCTC 1 cut(s) 1786
AccI GTMKAC 2 cut(s) 373, 2040
AciI CCGC 4 cut(s) 1684, 1716, 1786, 1801
AclWI GGATC 5 cut(s) 57, 227, 547, 614, 2018
AcsI RAATTY 4 cut(s) 117, 785, 1816, 2234
AfeI AGCGCT 1 cut(s) 1559
AfiI CCNNNNNNNGG 6 cut(s) 163, 483, 1622, 1646, 1848, 1939
AflIII ACRYGT 2 cut(s) 962, 1824
AgsI TTSAA 6 cut(s) 123, 152, 775, 1590, 2164, 2335
AjnI CCWGG 4 cut(s) 218, 402, 1639, 2219
AloI GAACNNNNNNTCC 2 cut(s) 1476, 1508
Alw21I GWGCWC 3 cut(s) 515, 738, 2193
Alw26I GTCTC 1 cut(s) 2333
Alw44I GTGCAC 1 cut(s) 2189
AlwI GGATC 5 cut(s) 57, 227, 547, 614, 2018
AlwNI CAGNNNCTG 1 cut(s) 1955
Ama87I CYCGRG 1 cut(s) 236
Aor51HI AGCGCT 1 cut(s) 1559
AoxI GGCC 5 cut(s) 81, 1079, 1125, 1648, 2002
ApaLI GTGCAC 1 cut(s) 2189
ApeKI GCWGC 7 cut(s) 139, 467, 1007, 1103, 1106, 1511, 2130
ApoI RAATTY 4 cut(s) 117, 785, 1816, 2234
Asp718I GGTACC 1 cut(s) 1636
AspLEI GCGC 2 cut(s) 1560, 2096
AspS9I GGNCC 2 cut(s) 1613, 1808
AsuC2I CCSGG 1 cut(s) 2135
AsuHPI GGTGA 5 cut(s) 464, 562, 1214, 1360, 1696
AsuNHI GCTAGC 1 cut(s) 1666
AvaI CYCGRG 1 cut(s) 236
AvaII GGWCC 2 cut(s) 1613, 1808
BaeGI GKGCMC 2 cut(s) 1448, 2193
BanI GGYRCC 1 cut(s) 1636
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 3 cut(s) 515, 738, 2193
BbvI GCAGC 7 cut(s) 151, 454, 994, 1090, 1093, 1523, 2142
BccI CCATC 7 cut(s) 477, 554, 851, 949, 1930, 1994, 2127
BceAI ACGGC 2 cut(s) 1259, 1341
BciT130I CCWGG 4 cut(s) 220, 404, 1641, 2221
BclI TGATCA 2 cut(s) 61, 1030
BcnI CCSGG 1 cut(s) 2135
BcoDI GTCTC 1 cut(s) 2333
BfaI CTAG 2 cut(s) 1667, 1965
BfmI CTRYAG 3 cut(s) 137, 316, 2208
BfoI RGCGCY 1 cut(s) 1561
BglII AGATCT 1 cut(s) 1858
BisI GCNGC 7 cut(s) 140, 468, 1008, 1104, 1107, 1512, 2131
BlpI GCTNAGC 1 cut(s) 887
BlsI GCNGC 7 cut(s) 141, 469, 1009, 1105, 1108, 1513, 2132
Bme1390I CCNGG 5 cut(s) 220, 404, 1641, 2135, 2221
Bme18I GGWCC 2 cut(s) 1613, 1808
BmeT110I CYCGRG 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 1613, 1808
BmiI GGNNCC 5 cut(s) 1494, 1615, 1638, 1790, 1810
BmrFI CCNGG 5 cut(s) 220, 404, 1641, 2135, 2221
BmrI ACTGGG 2 cut(s) 929, 1611
BmsI GCATC 4 cut(s) 1695, 1952, 2063, 2167
BmtI GCTAGC 1 cut(s) 1670
BmuI ACTGGG 2 cut(s) 929, 1611
BoxI GACNNNNGTC 1 cut(s) 333
BpiI GAAGAC 1 cut(s) 193
BpmI CTGGAG 1 cut(s) 558
Bpu1102I GCTNAGC 1 cut(s) 887
BpuEI CTTGAG 2 cut(s) 1454, 1946
BpuMI CCSGG 1 cut(s) 2135
Bsa29I ATCGAT 1 cut(s) 832
BsaJI CCNNGG 5 cut(s) 157, 218, 219, 237, 694
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 1476, 1506
Bsc4I CCNNNNNNNGG 6 cut(s) 163, 483, 1622, 1646, 1848, 1939
Bse1I ACTGG 6 cut(s) 25, 672, 935, 1617, 1632, 2044
Bse3DI GCAATG 2 cut(s) 1269, 1355
BseBI CCWGG 4 cut(s) 220, 404, 1641, 2221
BseCI ATCGAT 1 cut(s) 832
BseDI CCNNGG 5 cut(s) 157, 218, 219, 237, 694
BseGI GGATG 1 cut(s) 79
BseLI CCNNNNNNNGG 6 cut(s) 163, 483, 1622, 1646, 1848, 1939
BseMI GCAATG 2 cut(s) 1269, 1355
BseMII CTCAG 2 cut(s) 901, 1653
BseNI ACTGG 6 cut(s) 25, 672, 935, 1617, 1632, 2044
BseRI GAGGAG 4 cut(s) 92, 434, 728, 1457
BseSI GKGCMC 2 cut(s) 1448, 2193
BseXI GCAGC 7 cut(s) 151, 454, 994, 1090, 1093, 1523, 2142
BseYI CCCAGC 2 cut(s) 1283, 1488
BshFI GGCC 5 cut(s) 83, 1081, 1127, 1650, 2004
BshNI GGYRCC 1 cut(s) 1636
BshVI ATCGAT 1 cut(s) 832
BsiHKAI GWGCWC 3 cut(s) 515, 738, 2193
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 2 cut(s) 1921, 2135
BslFI GGGAC 8 cut(s) 507, 910, 923, 1336, 1599, 1757, 1915, 2075
BslI CCNNNNNNNGG 6 cut(s) 163, 483, 1622, 1646, 1848, 1939
BsmAI GTCTC 1 cut(s) 2333
BsmFI GGGAC 8 cut(s) 507, 910, 923, 1336, 1599, 1757, 1915, 2075
BsnI GGCC 5 cut(s) 83, 1081, 1127, 1650, 2004
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 4 cut(s) 515, 738, 1448, 2193
Bsp1407I TGTACA 2 cut(s) 1656, 1766
Bsp143I GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
Bsp1720I GCTNAGC 1 cut(s) 887
BspACI CCGC 4 cut(s) 1684, 1716, 1786, 1801
BspANI GGCC 5 cut(s) 83, 1081, 1127, 1650, 2004
BspCNI CTCAG 2 cut(s) 900, 1654
BspDI ATCGAT 1 cut(s) 832
BspHI TCATGA 3 cut(s) 1017, 1374, 2320
BspLI GGNNCC 5 cut(s) 1494, 1615, 1638, 1790, 1810
BspMAI CTGCAG 1 cut(s) 141
BspOI GCTAGC 1 cut(s) 1670
BspPI GGATC 5 cut(s) 57, 227, 547, 614, 2018
BspQI GCTCTTC 3 cut(s) 445, 503, 1776
BspT107I GGYRCC 1 cut(s) 1636
BsrBI CCGCTC 1 cut(s) 1786
BsrDI GCAATG 2 cut(s) 1269, 1355
BsrGI TGTACA 2 cut(s) 1656, 1766
BsrI ACTGG 6 cut(s) 25, 672, 935, 1617, 1632, 2044
BssECI CCNNGG 5 cut(s) 157, 218, 219, 237, 694
BssMI GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
BssT1I CCWWGG 1 cut(s) 157
Bst2UI CCWGG 4 cut(s) 220, 404, 1641, 2221
Bst4CI ACNGT 9 cut(s) 698, 1043, 1070, 1220, 1552, 1580, 1771, 2171, 2209
Bst6I CTCTTC 3 cut(s) 445, 503, 1776
BstAPI GCANNNNNTGC 3 cut(s) 914, 1145, 2102
BstAUI TGTACA 2 cut(s) 1656, 1766
BstC8I GCNNGC 5 cut(s) 144, 1161, 1668, 2076, 2128
BstDEI CTNAG 5 cut(s) 92, 887, 1662, 1986, 2276
BstDSI CCRYGG 1 cut(s) 694
BstEII GGTNACC 1 cut(s) 1348
BstENI CCTNNNNNAGG 2 cut(s) 1644, 1937
BstF5I GGATG 1 cut(s) 79
BstH2I RGCGCY 1 cut(s) 1561
BstHHI GCGC 2 cut(s) 1560, 2096
BstKTI GATC 8 cut(s) 52, 64, 235, 542, 609, 1033, 1861, 2026
BstMAI GTCTC 1 cut(s) 2333
BstMBI GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
BstMWI GCNNNNNNNGC 8 cut(s) 914, 1103, 1115, 1124, 1145, 1280, 1987, 2102
BstNI CCWGG 4 cut(s) 220, 404, 1641, 2221
BstNSI RCATGY 5 cut(s) 380, 500, 772, 966, 1828
BstPAI GACNNNNGTC 1 cut(s) 333
BstPI GGTNACC 1 cut(s) 1348
BstSCI CCNGG 5 cut(s) 218, 402, 1639, 2133, 2219
BstSFI CTRYAG 3 cut(s) 137, 316, 2208
BstSLI GKGCMC 2 cut(s) 1448, 2193
BstV1I GCAGC 7 cut(s) 151, 454, 994, 1090, 1093, 1523, 2142
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 539, 1858
BstYI RGATCY 2 cut(s) 539, 1858
Bsu15I ATCGAT 1 cut(s) 832
BsuRI GGCC 5 cut(s) 83, 1081, 1127, 1650, 2004
BsuTUI ATCGAT 1 cut(s) 832
BtgI CCRYGG 1 cut(s) 694
BtsCI GGATG 1 cut(s) 79
BtsI GCAGTG 1 cut(s) 1390
BtsIMutI CAGTG 2 cut(s) 1390, 1659
Cac8I GCNNGC 5 cut(s) 144, 1161, 1668, 2076, 2128
CaiI CAGNNNCTG 1 cut(s) 1955
CciI TCATGA 3 cut(s) 1017, 1374, 2320
CfoI GCGC 2 cut(s) 1560, 2096
Cfr13I GGNCC 2 cut(s) 1613, 1808
ClaI ATCGAT 1 cut(s) 832
CsiI ACCWGGT 1 cut(s) 1639
DdeI CTNAG 5 cut(s) 92, 887, 1662, 1986, 2276
DpnI GATC 8 cut(s) 51, 63, 234, 541, 608, 1032, 1860, 2025
DpnII GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
DraI TTTAAA 1 cut(s) 1291
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
Eam1104I CTCTTC 3 cut(s) 445, 503, 1776
EarI CTCTTC 3 cut(s) 445, 503, 1776
Eco130I CCWWGG 1 cut(s) 157
Eco147I AGGCCT 1 cut(s) 1650
Eco32I GATATC 2 cut(s) 1506, 2112
Eco47I GGWCC 2 cut(s) 1613, 1808
Eco47III AGCGCT 1 cut(s) 1559
Eco88I CYCGRG 1 cut(s) 236
Eco91I GGTNACC 1 cut(s) 1348
EcoNI CCTNNNNNAGG 2 cut(s) 1644, 1937
EcoO65I GGTNACC 1 cut(s) 1348
EcoRII CCWGG 4 cut(s) 218, 402, 1639, 2219
EcoRV GATATC 2 cut(s) 1506, 2112
EcoT14I CCWWGG 1 cut(s) 157
EcoT22I ATGCAT 2 cut(s) 502, 2272
ErhI CCWWGG 1 cut(s) 157
FaqI GGGAC 8 cut(s) 507, 910, 923, 1336, 1599, 1757, 1915, 2075
FauI CCCGC 2 cut(s) 1691, 1723
FauNDI CATATG 1 cut(s) 1203
FbaI TGATCA 2 cut(s) 61, 1030
FblI GTMKAC 2 cut(s) 373, 2040
Fnu4HI GCNGC 7 cut(s) 140, 468, 1008, 1104, 1107, 1512, 2131
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 7 cut(s) 140, 468, 1008, 1104, 1107, 1512, 2131
FspBI CTAG 2 cut(s) 1667, 1965
GlaI GCGC 2 cut(s) 1559, 2095
GluI GCNGC 7 cut(s) 140, 468, 1008, 1104, 1107, 1512, 2131
GsaI CCCAGC 2 cut(s) 1287, 1492
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 1 cut(s) 1561
HaeIII GGCC 5 cut(s) 83, 1081, 1127, 1650, 2004
HapII CCGG 2 cut(s) 1921, 2135
HhaI GCGC 2 cut(s) 1560, 2096
Hin6I GCGC 2 cut(s) 1558, 2094
HinP1I GCGC 2 cut(s) 1558, 2094
HincII GTYRAC 1 cut(s) 374
HindII GTYRAC 1 cut(s) 374
HinfI GANTC 9 cut(s) 565, 1120, 1188, 1600, 1928, 1952, 2063, 2081, 2326
HpaII CCGG 2 cut(s) 1921, 2135
HphI GGTGA 5 cut(s) 464, 562, 1214, 1360, 1696
Hpy188I TCNGA 4 cut(s) 627, 1030, 1951, 1957
HpyAV CCTTC 4 cut(s) 838, 987, 992, 1766
HpyCH4III ACNGT 9 cut(s) 698, 1043, 1070, 1220, 1552, 1580, 1771, 2171, 2209
HpyCH4IV ACGT 1 cut(s) 798
HpyF10VI GCNNNNNNNGC 8 cut(s) 914, 1103, 1115, 1124, 1145, 1280, 1987, 2102
HpyF3I CTNAG 5 cut(s) 92, 887, 1662, 1986, 2276
HpySE526I ACGT 1 cut(s) 798
HspAI GCGC 2 cut(s) 1558, 2094
KpnI GGTACC 1 cut(s) 1640
Ksp22I TGATCA 2 cut(s) 61, 1030
Kzo9I GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
LguI GCTCTTC 3 cut(s) 445, 503, 1776
LmnI GCTCC 3 cut(s) 251, 421, 741
Lsp1109I GCAGC 7 cut(s) 151, 454, 994, 1090, 1093, 1523, 2142
LweI GCATC 4 cut(s) 1695, 1952, 2063, 2167
MabI ACCWGGT 1 cut(s) 1639
MaeI CTAG 2 cut(s) 1667, 1965
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 5 cut(s) 363, 617, 1220, 1276, 1348
MalI GATC 8 cut(s) 51, 63, 234, 541, 608, 1032, 1860, 2025
MbiI CCGCTC 1 cut(s) 1786
MboI GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 2 cut(s) 539, 1858
MhlI GDGCHC 4 cut(s) 515, 738, 1448, 2193
MlyI GAGTC 6 cut(s) 1129, 1197, 1937, 1946, 2057, 2335
MmeI TCCRAC 1 cut(s) 468
Mph1103I ATGCAT 2 cut(s) 502, 2272
MseI TTAA 8 cut(s) 245, 387, 803, 840, 1155, 1245, 1290, 2310
MslI CAYNNNNRTG 1 cut(s) 1274
MspA1I CMGCKG 2 cut(s) 1283, 2078
MspI CCGG 2 cut(s) 1921, 2135
MspR9I CCNGG 5 cut(s) 220, 404, 1641, 2135, 2221
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 4 cut(s) 220, 404, 1641, 2221
MwoI GCNNNNNNNGC 8 cut(s) 914, 1103, 1115, 1124, 1145, 1280, 1987, 2102
NciI CCSGG 1 cut(s) 2135
NdeI CATATG 1 cut(s) 1203
NdeII GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
NheI GCTAGC 1 cut(s) 1666
NlaIV GGNNCC 5 cut(s) 1494, 1615, 1638, 1790, 1810
NmuCI GTSAC 4 cut(s) 617, 1220, 1276, 1348
NsiI ATGCAT 2 cut(s) 502, 2272
NspI RCATGY 5 cut(s) 380, 500, 772, 966, 1828
PagI TCATGA 3 cut(s) 1017, 1374, 2320
PasI CCCWGGG 1 cut(s) 219
PceI AGGCCT 1 cut(s) 1650
PciI ACATGT 2 cut(s) 962, 1824
PciSI GCTCTTC 3 cut(s) 445, 503, 1776
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PfeI GAWTC 3 cut(s) 565, 1600, 2081
PflFI GACNNNGTC 1 cut(s) 1611
PflMI CCANNNNNTGG 1 cut(s) 483
PkrI GCNGC 7 cut(s) 141, 469, 1009, 1105, 1108, 1513, 2132
PleI GAGTC 6 cut(s) 1128, 1196, 1936, 1946, 2057, 2334
PpsI GAGTC 6 cut(s) 1128, 1196, 1936, 1946, 2057, 2334
PscI ACATGT 2 cut(s) 962, 1824
PshAI GACNNNNGTC 1 cut(s) 333
Psp6I CCWGG 4 cut(s) 218, 402, 1639, 2219
PspEI GGTNACC 1 cut(s) 1348
PspFI CCCAGC 2 cut(s) 1283, 1488
PspGI CCWGG 4 cut(s) 218, 402, 1639, 2219
PspN4I GGNNCC 5 cut(s) 1494, 1615, 1638, 1790, 1810
PspPI GGNCC 2 cut(s) 1613, 1808
PstI CTGCAG 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 1955
PsuI RGATCY 2 cut(s) 539, 1858
PsyI GACNNNGTC 1 cut(s) 1611
PvuII CAGCTG 2 cut(s) 1283, 2078
RseI CAYNNNNRTG 1 cut(s) 1274
SalI GTCGAC 1 cut(s) 372
SapI GCTCTTC 3 cut(s) 445, 503, 1776
SaqAI TTAA 8 cut(s) 245, 387, 803, 840, 1155, 1245, 1290, 2310
SatI GCNGC 7 cut(s) 140, 468, 1008, 1104, 1107, 1512, 2131
Sau3AI GATC 8 cut(s) 49, 61, 232, 539, 606, 1030, 1858, 2023
Sau96I GGNCC 2 cut(s) 1613, 1808
SchI GAGTC 6 cut(s) 1129, 1197, 1937, 1946, 2057, 2335
ScrFI CCNGG 5 cut(s) 220, 404, 1641, 2135, 2221
SduI GDGCHC 4 cut(s) 515, 738, 1448, 2193
SexAI ACCWGGT 1 cut(s) 1639
SfaNI GCATC 4 cut(s) 1695, 1952, 2063, 2167
SfcI CTRYAG 3 cut(s) 137, 316, 2208
SinI GGWCC 2 cut(s) 1613, 1808
SmiMI CAYNNNNRTG 1 cut(s) 1274
SmlI CTYRAG 2 cut(s) 1469, 1925
SmoI CTYRAG 2 cut(s) 1469, 1925
SseBI AGGCCT 1 cut(s) 1650
SsiI CCGC 4 cut(s) 1684, 1716, 1786, 1801
SspMI CTAG 2 cut(s) 1667, 1965
StuI AGGCCT 1 cut(s) 1650
StyD4I CCNGG 5 cut(s) 218, 402, 1639, 2133, 2219
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 9 cut(s) 698, 1043, 1070, 1220, 1552, 1580, 1771, 2171, 2209
TaiI ACGT 1 cut(s) 801
TaqI TCGA 3 cut(s) 373, 789, 832
TatI WGTACW 3 cut(s) 1462, 1656, 1766
TfiI GAWTC 3 cut(s) 565, 1600, 2081
Tru1I TTAA 8 cut(s) 245, 387, 803, 840, 1155, 1245, 1290, 2310
Tru9I TTAA 8 cut(s) 245, 387, 803, 840, 1155, 1245, 1290, 2310
TscAI CASTG 2 cut(s) 1397, 1666
TseFI GTSAC 4 cut(s) 617, 1220, 1276, 1348
TseI GCWGC 7 cut(s) 139, 467, 1007, 1103, 1106, 1511, 2130
Tsp45I GTSAC 4 cut(s) 617, 1220, 1276, 1348
TspDTI ATGAA 6 cut(s) 27, 521, 1034, 1712, 1900, 2076
TspGWI ACGGA 2 cut(s) 605, 1820
TspRI CASTG 2 cut(s) 1397, 1666
Tth111I GACNNNGTC 1 cut(s) 1611
Van91I CCANNNNNTGG 1 cut(s) 483
VneI GTGCAC 1 cut(s) 2189
VpaK11BI GGWCC 2 cut(s) 1613, 1808
XagI CCTNNNNNAGG 2 cut(s) 1644, 1937
XapI RAATTY 4 cut(s) 117, 785, 1816, 2234
XceI RCATGY 5 cut(s) 380, 500, 772, 966, 1828
XcmI CCANNNNNNNNNTGG 3 cut(s) 484, 1245, 1624
XmiI GTMKAC 2 cut(s) 373, 2040
XspI CTAG 2 cut(s) 1667, 1965
Zsp2I ATGCAT 2 cut(s) 502, 2272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.