Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0000477

Physical Location & Seq

Genomic Coordinates Reverse (-)

24935943 .. 24937387

Gene Structure 1445 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g22730.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1107 bp

ATGATTCTTTGCATTGCTGTGACTGCTGGGTTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCAGTTGTAATAGTCATGCTGGCAACCACATTGCTTATGACTCTAATCATGATATTAGTGTGGCGCTGCCATTGGATTCTTGTCGTTACATTCACCAGCTTGTCACTGATTGTGGAATGCACTTATTTTTCCGCTGTACTCTGCAAGGTTGATCAAGGTGGATGGGTTCCTCTTGTGATTGCAGCTGCTTTTCTTCTCATCATGTATGTTTGGCATTATGGTACGTTGAAACGTTATGAGTTTGAGATGCACAGTAAAGTTTCAATGGCATGGCTTCTTGGGCTTGGCCCCAGTTTAGGACTGGTTCGGGTCCCTGGGATAGGACTTGTGTACACCGAGCTTGCAAGTGGGGTGCCCCACATCTTTTCCCACTTCATCACCAACCTGCCTGCAATCCACCAGGTTGTTGTCTTTGTCTGTGTTAAGTACCTCCCTGTATACACCGTCCCAGAAGAAGAAAGATTCCTTGTCAAGCGGATTGGCCCCAAGAGTTTCCACATGTTTCGTTGTGTTGCAAGATACGGTTATAAAGACCTACACAAGAAAGATGACGACTTTGAGAAGAAGCTCTTTGAAAACCTTTTCCTGTTTGTCCGGCTCGAGTCCATGATGGAAGGGTGTTCAGACTCAGATGAGTACAGCATCAATGGCCAGCAAACTGAGAGATCAAGGGACGGCCTCTTAACCAATAATGGCAACACAACCACTTCTACCGTAGACCTCAGTATCTTATCAGTGGACTCAATTGTTCCGGCTAAATCTCCACCGCACGCGAACAACACCGTGAGTTCGTACGGGCAGATGAGCAGCCAGAATGAGATTGATGAGCTAGAGTTTCTGAATAATTGTCGAGAGTCCGGGGTGGTGCATATACTTGGGAACACGGTAGTGAGAGCAAGGAGGGATTCCAGGTTCTACAAAAAGATAGCCGTTGATTACATTTATGCATTTCTCAGGAAAATTTGCAGGGAGAATAGTGTGATCTTCAACGTTCCTCATGAGAGTCTATTGAATGTTGGACAGATTTTCTATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

369

Amino Acids

41.6

Weight (kDa)

7.6

Isoelectric Point (pI)

39.93

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca	FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica	MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica	Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis	pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis	RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata	RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora	Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa	Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha	Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana	Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	597
Acc36I	ACCTGC	1 cut(s)	462
AccB1I	GGYRCC	1 cut(s)	421
AccI	GTMKAC	2 cut(s)	507, 786
AccII	CGCG	1 cut(s)	842
AciI	CCGC	3 cut(s)	201, 544, 836
AclI	AACGTT	2 cut(s)	301, 1059
AcoI	YGGCCR	1 cut(s)	718
AcsI	RAATTY	1 cut(s)	1029
AfaI	GTAC	6 cut(s)	207, 292, 401, 497, 707, 863
AfiI	CCNNNNNNNGG	2 cut(s)	365, 389
AflIII	ACRYGT	1 cut(s)	567
AgsI	TTSAA	6 cut(s)	34, 298, 333, 644, 1057, 1081
AjnI	CCWGG	3 cut(s)	382, 468, 977
AleI	CACNNNNGTG	1 cut(s)	956
AloI	GAACNNNNNNTCC	2 cut(s)	965, 997
AluBI	AGCT	5 cut(s)	168, 254, 409, 637, 898
AluI	AGCT	5 cut(s)	168, 254, 409, 637, 898
Ama87I	CYCGRG	1 cut(s)	668
AoxI	GGCC	4 cut(s)	355, 550, 718, 745
ApeKI	GCWGC	4 cut(s)	135, 251, 254, 876
ApoI	RAATTY	1 cut(s)	1029
AspLEI	GCGC	1 cut(s)	135
AspS9I	GGNCC	3 cut(s)	356, 379, 551
AsuC2I	CCSGG	1 cut(s)	928
AsuHPI	GGTGA	2 cut(s)	154, 439
AvaI	CYCGRG	1 cut(s)	668
AvaII	GGWCC	1 cut(s)	379
BaeGI	GKGCMC	1 cut(s)	426
BaeI	ACNNNNGTAYC	2 cut(s)	778, 811
BalI	TGGCCA	1 cut(s)	720
BanI	GGYRCC	1 cut(s)	421
BbvI	GCAGC	4 cut(s)	122, 241, 263, 888
BccI	CCATC	2 cut(s)	225, 673
BceAI	ACGGC	3 cut(s)	84, 760, 983
BciT130I	CCWGG	3 cut(s)	384, 470, 979
BclI	TGATCA	1 cut(s)	220
BcnI	CCSGG	1 cut(s)	928
BfaI	CTAG	1 cut(s)	899
BfoI	RGCGCY	1 cut(s)	136
BfuAI	ACCTGC	1 cut(s)	462
BisI	GCNGC	4 cut(s)	136, 252, 255, 877
BlsI	GCNGC	4 cut(s)	137, 253, 256, 878
Bme1390I	CCNGG	4 cut(s)	384, 470, 928, 979
Bme18I	GGWCC	1 cut(s)	379
BmeT110I	CYCGRG	1 cut(s)	668
BmgT120I	GGNCC	3 cut(s)	356, 379, 551
BmiI	GGNNCC	6 cut(s)	237, 358, 380, 381, 423, 553
BmrFI	CCNGG	4 cut(s)	384, 470, 928, 979
BmrI	ACTGGG	1 cut(s)	354
BmsI	GCATC	2 cut(s)	306, 720
BmuI	ACTGGG	1 cut(s)	354
BpuMI	CCSGG	1 cut(s)	928
BsaJI	CCNNGG	3 cut(s)	382, 383, 927
Bsc4I	CCNNNNNNNGG	2 cut(s)	365, 389
Bse1I	ACTGG	2 cut(s)	360, 375
Bse3DI	GCAATG	2 cut(s)	12, 98
BseBI	CCWGG	3 cut(s)	384, 470, 979
BseDI	CCNNGG	3 cut(s)	382, 383, 927
BseGI	GGATG	1 cut(s)	236
BseLI	CCNNNNNNNGG	2 cut(s)	365, 389
BseMI	GCAATG	2 cut(s)	12, 98
BseMII	CTCAG	4 cut(s)	711, 720, 805, 1036
BseNI	ACTGG	2 cut(s)	360, 375
BseSI	GKGCMC	1 cut(s)	426
BseXI	GCAGC	4 cut(s)	122, 241, 263, 888
BseYI	CCCAGC	1 cut(s)	26
Bsh1236I	CGCG	1 cut(s)	842
BshFI	GGCC	4 cut(s)	357, 552, 720, 747
BshNI	GGYRCC	1 cut(s)	421
BsiHKCI	CYCGRG	1 cut(s)	668
BsiSI	CCGG	3 cut(s)	664, 821, 927
BsiWI	CGTACG	1 cut(s)	861
BslFI	GGGAC	4 cut(s)	79, 365, 500, 755
BslI	CCNNNNNNNGG	2 cut(s)	365, 389
BsmFI	GGGAC	4 cut(s)	79, 365, 500, 755
BsmI	GAATGC	1 cut(s)	191
BsnI	GGCC	4 cut(s)	357, 552, 720, 747
BsoBI	CYCGRG	1 cut(s)	668
Bsp1286I	GDGCHC	1 cut(s)	426
Bsp1407I	TGTACA	1 cut(s)	399
Bsp143I	GATC	3 cut(s)	220, 734, 1050
BspACI	CCGC	3 cut(s)	201, 544, 836
BspANI	GGCC	4 cut(s)	357, 552, 720, 747
BspCNI	CTCAG	4 cut(s)	710, 721, 804, 1035
BspFNI	CGCG	1 cut(s)	842
BspHI	TCATGA	2 cut(s)	117, 1066
BspLI	GGNNCC	6 cut(s)	237, 358, 380, 381, 423, 553
BspMI	ACCTGC	1 cut(s)	462
BspT107I	GGYRCC	1 cut(s)	421
BsrDI	GCAATG	2 cut(s)	12, 98
BsrGI	TGTACA	1 cut(s)	399
BsrI	ACTGG	2 cut(s)	360, 375
BssECI	CCNNGG	3 cut(s)	382, 383, 927
BssMI	GATC	3 cut(s)	220, 734, 1050
BssNAI	GTATAC	1 cut(s)	508
Bst1107I	GTATAC	1 cut(s)	508
Bst2UI	CCWGG	3 cut(s)	384, 470, 979
Bst4CI	ACNGT	6 cut(s)	323, 514, 593, 784, 853, 955
BstAUI	TGTACA	1 cut(s)	399
BstC8I	GCNNGC	5 cut(s)	90, 411, 459, 722, 840
BstDEI	CTNAG	4 cut(s)	697, 729, 791, 1022
BstENI	CCTNNNNNAGG	1 cut(s)	387
BstF5I	GGATG	1 cut(s)	236
BstFNI	CGCG	1 cut(s)	842
BstH2I	RGCGCY	1 cut(s)	136
BstHHI	GCGC	1 cut(s)	135
BstKTI	GATC	3 cut(s)	223, 737, 1053
BstMBI	GATC	3 cut(s)	220, 734, 1050
BstMWI	GCNNNNNNNGC	3 cut(s)	23, 349, 717
BstNI	CCWGG	3 cut(s)	384, 470, 979
BstNSI	RCATGY	1 cut(s)	571
BstSCI	CCNGG	4 cut(s)	382, 468, 926, 977
BstSLI	GKGCMC	1 cut(s)	426
BstUI	CGCG	1 cut(s)	842
BstV1I	GCAGC	4 cut(s)	122, 241, 263, 888
BstZ17I	GTATAC	1 cut(s)	508
BsuRI	GGCC	4 cut(s)	357, 552, 720, 747
BtsCI	GGATG	1 cut(s)	236
BtsIMutI	CAGTG	2 cut(s)	173, 810
BveI	ACCTGC	1 cut(s)	462
Cac8I	GCNNGC	5 cut(s)	90, 411, 459, 722, 840
CciI	TCATGA	2 cut(s)	117, 1066
CfoI	GCGC	1 cut(s)	135
Cfr13I	GGNCC	3 cut(s)	356, 379, 551
CsiI	ACCWGGT	1 cut(s)	468
Csp6I	GTAC	6 cut(s)	206, 291, 400, 496, 706, 862
CviAII	CATG	7 cut(s)	85, 118, 271, 339, 568, 676, 1067
CviQI	GTAC	6 cut(s)	206, 291, 400, 496, 706, 862
DdeI	CTNAG	4 cut(s)	697, 729, 791, 1022
DpnI	GATC	3 cut(s)	222, 736, 1052
DpnII	GATC	3 cut(s)	220, 734, 1050
EaeI	YGGCCR	1 cut(s)	718
Eco47I	GGWCC	1 cut(s)	379
Eco88I	CYCGRG	1 cut(s)	668
EcoNI	CCTNNNNNAGG	1 cut(s)	387
EcoO109I	RGGNCCY	1 cut(s)	379
EcoRII	CCWGG	3 cut(s)	382, 468, 977
EcoT22I	ATGCAT	1 cut(s)	1018
FaeI	CATG	7 cut(s)	88, 121, 274, 342, 571, 679, 1070
FalI	AAGNNNNNCTT	2 cut(s)	623, 655
FaqI	GGGAC	4 cut(s)	79, 365, 500, 755
FatI	CATG	7 cut(s)	84, 117, 270, 338, 567, 675, 1066
FbaI	TGATCA	1 cut(s)	220
FblI	GTMKAC	2 cut(s)	507, 786
Fnu4HI	GCNGC	4 cut(s)	136, 252, 255, 877
FokI	GGATG	1 cut(s)	243
Fsp4HI	GCNGC	4 cut(s)	136, 252, 255, 877
FspBI	CTAG	1 cut(s)	899
GlaI	GCGC	1 cut(s)	134
GluI	GCNGC	4 cut(s)	136, 252, 255, 877
GsaI	CCCAGC	1 cut(s)	30
HaeII	RGCGCY	1 cut(s)	136
HaeIII	GGCC	4 cut(s)	357, 552, 720, 747
HapII	CCGG	3 cut(s)	664, 821, 927
HhaI	GCGC	1 cut(s)	135
Hin1II	CATG	7 cut(s)	88, 121, 274, 342, 571, 679, 1070
Hin6I	GCGC	1 cut(s)	133
HinP1I	GCGC	1 cut(s)	133
HpaII	CCGG	3 cut(s)	664, 821, 927
HphI	GGTGA	2 cut(s)	154, 439
Hpy166II	GTNNAC	5 cut(s)	400, 402, 508, 787, 808
Hpy188I	TCNGA	3 cut(s)	694, 700, 909
Hpy188III	TCNNGA	4 cut(s)	118, 920, 1024, 1067
Hpy8I	GTNNAC	5 cut(s)	400, 402, 508, 787, 808
HpyAV	CCTTC	1 cut(s)	677
HpyCH4III	ACNGT	6 cut(s)	323, 514, 593, 784, 853, 955
HpyCH4IV	ACGT	3 cut(s)	293, 301, 1059
HpyF10VI	GCNNNNNNNGC	3 cut(s)	23, 349, 717
HpyF3I	CTNAG	4 cut(s)	697, 729, 791, 1022
HpySE526I	ACGT	3 cut(s)	293, 301, 1059
Hsp92II	CATG	7 cut(s)	88, 121, 274, 342, 571, 679, 1070
HspAI	GCGC	1 cut(s)	133
KflI	GGGWCCC	1 cut(s)	379
Ksp22I	TGATCA	1 cut(s)	220
Kzo9I	GATC	3 cut(s)	220, 734, 1050
Lsp1109I	GCAGC	4 cut(s)	122, 241, 263, 888
LweI	GCATC	2 cut(s)	306, 720
MabI	ACCWGGT	1 cut(s)	468
MaeI	CTAG	1 cut(s)	899
MaeII	ACGT	3 cut(s)	293, 301, 1059
MaeIII	GTNAC	3 cut(s)	19, 154, 171
MalI	GATC	3 cut(s)	222, 736, 1052
MboI	GATC	3 cut(s)	220, 734, 1050
MboII	GAAGA	5 cut(s)	254, 533, 536, 643, 1045
MfeI	CAATTG	1 cut(s)	813
MhlI	GDGCHC	1 cut(s)	426
MlsI	TGGCCA	1 cut(s)	720
MluCI	AATT	4 cut(s)	48, 813, 913, 1029
MluNI	TGGCCA	1 cut(s)	720
MlyI	GAGTC	6 cut(s)	103, 680, 689, 803, 932, 1081
MmeI	TCCRAC	1 cut(s)	1066
MnlI	CCTC	6 cut(s)	249, 509, 758, 800, 963, 1074
Mox20I	TGGCCA	1 cut(s)	720
Mph1103I	ATGCAT	1 cut(s)	1018
MscI	TGGCCA	1 cut(s)	720
MseI	TTAA	2 cut(s)	492, 752
MslI	CAYNNNNRTG	2 cut(s)	17, 956
Msp20I	TGGCCA	1 cut(s)	720
MspA1I	CMGCKG	2 cut(s)	203, 254
MspI	CCGG	3 cut(s)	664, 821, 927
MspR9I	CCNGG	4 cut(s)	384, 470, 928, 979
MunI	CAATTG	1 cut(s)	813
Mva1269I	GAATGC	1 cut(s)	191
MvaI	CCWGG	3 cut(s)	384, 470, 979
MvnI	CGCG	1 cut(s)	842
MwoI	GCNNNNNNNGC	3 cut(s)	23, 349, 717
NciI	CCSGG	1 cut(s)	928
NdeII	GATC	3 cut(s)	220, 734, 1050
NlaIII	CATG	7 cut(s)	88, 121, 274, 342, 571, 679, 1070
NlaIV	GGNNCC	6 cut(s)	237, 358, 380, 381, 423, 553
NmuCI	GTSAC	2 cut(s)	19, 171
NsiI	ATGCAT	1 cut(s)	1018
NspI	RCATGY	1 cut(s)	571
OliI	CACNNNNGTG	1 cut(s)	956
PaeR7I	CTCGAG	1 cut(s)	668
PagI	TCATGA	2 cut(s)	117, 1066
PasI	CCCWGGG	1 cut(s)	383
PciI	ACATGT	1 cut(s)	567
PctI	GAATGC	1 cut(s)	191
PfeI	GAWTC	4 cut(s)	4, 145, 531, 974
Pfl23II	CGTACG	1 cut(s)	861
PkrI	GCNGC	4 cut(s)	137, 253, 256, 878
PleI	GAGTC	6 cut(s)	103, 679, 689, 803, 931, 1080
PpsI	GAGTC	6 cut(s)	103, 679, 689, 803, 931, 1080
PpuMI	RGGWCCY	1 cut(s)	379
PscI	ACATGT	1 cut(s)	567
PsiI	TTATAA	1 cut(s)	597
Psp1406I	AACGTT	2 cut(s)	301, 1059
Psp5II	RGGWCCY	1 cut(s)	379
Psp6I	CCWGG	3 cut(s)	382, 468, 977
PspFI	CCCAGC	1 cut(s)	26
PspGI	CCWGG	3 cut(s)	382, 468, 977
PspLI	CGTACG	1 cut(s)	861
PspN4I	GGNNCC	6 cut(s)	237, 358, 380, 381, 423, 553
PspPI	GGNCC	3 cut(s)	356, 379, 551
PspPPI	RGGWCCY	1 cut(s)	379
PspXI	VCTCGAGB	1 cut(s)	668
PvuII	CAGCTG	1 cut(s)	254
RsaI	GTAC	6 cut(s)	207, 292, 401, 497, 707, 863
RsaNI	GTAC	6 cut(s)	206, 291, 400, 496, 706, 862
RseI	CAYNNNNRTG	2 cut(s)	17, 956
SaqAI	TTAA	2 cut(s)	492, 752
SatI	GCNGC	4 cut(s)	136, 252, 255, 877
Sau3AI	GATC	3 cut(s)	220, 734, 1050
Sau96I	GGNCC	3 cut(s)	356, 379, 551
SchI	GAGTC	6 cut(s)	103, 680, 689, 803, 932, 1081
ScrFI	CCNGG	4 cut(s)	384, 470, 928, 979
SduI	GDGCHC	1 cut(s)	426
SexAI	ACCWGGT	1 cut(s)	468
SfaNI	GCATC	2 cut(s)	306, 720
Sfr274I	CTCGAG	1 cut(s)	668
SinI	GGWCC	1 cut(s)	379
SlaI	CTCGAG	1 cut(s)	668
SmiMI	CAYNNNNRTG	2 cut(s)	17, 956
SmlI	CTYRAG	1 cut(s)	668
SmoI	CTYRAG	1 cut(s)	668
Sse9I	AATT	4 cut(s)	48, 813, 913, 1029
SsiI	CCGC	3 cut(s)	201, 544, 836
SspMI	CTAG	1 cut(s)	899
StyD4I	CCNGG	4 cut(s)	382, 468, 926, 977
TaaI	ACNGT	6 cut(s)	323, 514, 593, 784, 853, 955
TaiI	ACGT	3 cut(s)	296, 304, 1062
TaqI	TCGA	2 cut(s)	669, 919
TasI	AATT	4 cut(s)	48, 813, 913, 1029
TatI	WGTACW	3 cut(s)	205, 399, 705
TfiI	GAWTC	4 cut(s)	4, 145, 531, 974
Tru1I	TTAA	2 cut(s)	492, 752
Tru9I	TTAA	2 cut(s)	492, 752
TscAI	CASTG	2 cut(s)	180, 810
TseFI	GTSAC	2 cut(s)	19, 171
TseI	GCWGC	4 cut(s)	135, 251, 254, 876
Tsp45I	GTSAC	2 cut(s)	19, 171
TspDTI	ATGAA	1 cut(s)	433
TspRI	CASTG	2 cut(s)	180, 810
VpaK11BI	GGWCC	1 cut(s)	379
XagI	CCTNNNNNAGG	1 cut(s)	387
XapI	RAATTY	1 cut(s)	1029
XceI	RCATGY	1 cut(s)	571
XcmI	CCANNNNNNNNNTGG	1 cut(s)	367
XhoI	CTCGAG	1 cut(s)	668
XmiI	GTMKAC	2 cut(s)	507, 786
XspI	CTAG	1 cut(s)	899
Zsp2I	ATGCAT	1 cut(s)	1018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.