FvH4_3g12500

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000477
Physical Location & Seq
Forward (+)
7442246 .. 7449349
7104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g12500.t12

Sequence Viewer

Length: 2382 bp
ATGAATTTAGGAGGGGATATTAGTGAAGAAAATGATACCAACAAAGGCAGTATGTGGGTTTTGGATCAGAAGCTTGACCAACCCATGGATGAGGAGGCTGGCAGGCTTAGAACTATGTACAGGGAAAAAACATTCTCCTCGCTATTGCTTCTTCGGTTTGCATTCCAAAGTCTTGGAGTGGTTTTTGGAGATTTAGGCACTTCCCCCTTGTATGTTTTCTACAATACATTTCCTCGTGGAATTTCAGATCCTGAGGATGTGGTTGGAGCTCTTTCATTGATTATATACTCTCTTACTCTTATCCCACTCATCAAGTACATATTTGTTGTTTGTCGGGCAAACGACAGCGGGCAAGGTGGAACATTTGCTCTTTATTCATTGCTCTGTCGGCATGCCAAAATAAAAACTATTCCCAATCAGCACCGGACCGATGAAGAGCTAACAACATATAGTCGTTCTACTTTTCATGAACAATCGTACGCTGCAAAAACAAAGAAGTGGTTGGAAGGGCATGCATCAAGGAAGAATGCACTTCTTGTTCTGGTCCTTGTTGGCACTTGTATGGTTATTGGAGATGGGATTCTCACTCCAGCTATATCAGTTCTATCAGCTGCTGGGGGGATCAAAGTAAACAGTCCTAAGATGAATAATGATTATGTCATACTTGTAGCCGTTGTGATATTAGTAGGTTTGTTCAGCATGCAGCATTATGGGACAGATAAAGTTGGTTGGCTCTTTGCTCCAATTGTGTTGCTTTGGTTTCTCTTGATTGGAGGTATTGGCATATTCAACATCTGGAAATATGACAGTAGTGTTTTGAGAGCTTTTTCACCTGTGTATATATATCGATATTTTAAAAGGAATGGGAAAGAGGGTTGGACTTCCCTTGGAGGTATTATGCTTAGTATAACAGGGACAGAAGCACTCTTTGCCGACCTTGCCCATTTTCCAGTTTCAGCTGTACAGATTGCTTTTACCACAGTTGTGTTTCCTTGCCTTCTTTTAGCCTATTCTGGACAAGCTGCCTTTCTTGTAGTACATCATGATAACGATGAAACTGTGACTCAAGCATTTTATCGTTCTATTCCAGAGAAGATATATTGGCCAGTCTTCGTTGTTGCGACTCTAGCTGCCATAGTTGCAAGTCAAGCAACTATATCTGCAACTTTTTCAATAATTAAGCAGGCTCATGCTCTTGGCTGTTTTCCCAGGGTTAAAGTTGTACATACATCAAAGAAGTTCCTCGGCCAAATATATATTCCAGATATTAATTGGATCCTTATGATTCTTTGCATTGCTGTGACTGCTGGATTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCGGTTGTGGTGGTCATGCTGGTAACCACTTTGCTTATGACTCTAATCATGATATTGGTGTGGCGCTGCCATTGGATTCTTGTCGTCATATTCACTGGTTTGTCCCTGGTTGTGGAGTGCACTTACTTTTCTGCTGTACTCTTCAAGGTTGATCAAGGGGGTTGGGTTCCTCTTGTGATTGCAGCTGCCTTTCTTATCATCATGTATGTATGGCATTATGGATCTTTGAAACGTTATGAGTTTGAAATGCACAGTAGGGTTTCAATGGCTTGGCTTCTTGGACTTGGCCCCAGTTTAGGATTGGTTCGGGTCCCTGGGGTTGGACTTGTGTATACCGAGCTTGCTAGCGGGGTGCCCCATATCTTTTCCCACTTCATCACCAACCTGCCTGCTATCCATTCTGTTGTTGTCTTTGTTTGTGTGAAGTATCTTCCTGTGTATACTGTCCCAGAAGAAGAAAGATTCCTTGTAAAACGAATTGGCCCCAAGAATTTCCACATGTTCCGGTGTGTTGCAAGATATGGTTACAAAGACCTCCACCGAAAAGATGATGACTTTGAGAAAAAACTGTTTGATAACCTTTTCATGTTTGTTCGACTGGAGGCCATGATGGAAGGGTGTTCAGACTCGGATGAGTACAGTTTATATGGCCAGCAAACTGAGAAGTCGAGAGAAAACCTATTAAACAATAATGGAAACACTTCCCCATCCATCGGGGACTTTACCATGTCGACAGTGGATTCCATTGTGCCTGTTAGATCTCCAGTGAATGGGAACAACACAGTCACTTCTCAGATGAGCAACCAGACTGAGATGGATGAGCTAGAATTTATGAACAATTGTAGAGACGCCGGGGTGGTGCATATACTTGGGAATACAGTAGTGAGAGCAAGGAGGGATTCCAGGTTCTACAAGAAGATAGCTGTTGATTACATATATGCATTTCTTAGAAAAGTATGCAGGGAGCACAGTGTGATCTTCAATGTCCCTCATGAGAGTCTTTTGAATGTTGGACAAATTTTCTACGTGTAA

Protein Analysis

794

Amino Acids

88.9

Weight (kDa)

8.38

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 18 - 505 3.4e-163 K+ potassium transporter integral membrane domain
K_trans_C PF22776 519 - 754 2.1e-25 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1743
AccB1I GGYRCC 1 cut(s) 1702
AccB7I CCANNNNNTGG 2 cut(s) 85, 2120
AccI GTMKAC 3 cut(s) 1682, 1790, 2081
AciI CCGC 3 cut(s) 348, 1352, 1698
AclI AACGTT 1 cut(s) 1582
AclWI GGATC 6 cut(s) 72, 242, 629, 1270, 1283, 1579
AcoI YGGCCR 3 cut(s) 1103, 1246, 1999
AcsI RAATTY 5 cut(s) 4, 240, 1840, 2177, 2367
AcyI GRCGYC 1 cut(s) 2199
AdeI CACNNNGTG 1 cut(s) 2323
AfaI GTAC 8 cut(s) 119, 317, 479, 963, 1038, 1224, 1488, 1988
AfiI CCNNNNNNNGG 6 cut(s) 85, 1462, 1646, 1670, 2063, 2120
AflIII ACRYGT 2 cut(s) 1848, 2376
AgsI TTSAA 9 cut(s) 790, 1173, 1315, 1495, 1579, 1595, 1614, 2332, 2356
AjnI CCWGG 4 cut(s) 1208, 1455, 1663, 2252
AjuI GAANNNNNNNTTGG 2 cut(s) 1242, 1274
AleI CACNNNNGTG 1 cut(s) 983
AloI GAACNNNNNNTCC 2 cut(s) 2240, 2272
Alw21I GWGCWC 3 cut(s) 271, 1472, 2319
Alw26I GTCTC 1 cut(s) 2190
Alw44I GTGCAC 1 cut(s) 1468
AlwI GGATC 6 cut(s) 72, 242, 629, 1270, 1283, 1579
AlwNI CAGNNNCTG 2 cut(s) 251, 614
AoxI GGCC 6 cut(s) 1103, 1246, 1636, 1831, 1953, 1999
ApaLI GTGCAC 1 cut(s) 1468
ApeKI GCWGC 8 cut(s) 482, 611, 703, 1022, 1130, 1416, 1532, 1535
ApoI RAATTY 5 cut(s) 4, 240, 1840, 2177, 2367
AseI ATTAAT 1 cut(s) 1269
Asp700I GAANNNNTTC 1 cut(s) 2050
AspLEI GCGC 1 cut(s) 1416
AspS9I GGNCC 5 cut(s) 426, 544, 1637, 1660, 1832
AsuC2I CCSGG 1 cut(s) 2203
AsuHPI GGTGA 2 cut(s) 822, 1720
AsuNHI GCTAGC 1 cut(s) 1694
AvaII GGWCC 3 cut(s) 426, 544, 1660
AxyI CCTNAGG 1 cut(s) 252
BaeGI GKGCMC 2 cut(s) 1472, 1707
BalI TGGCCA 2 cut(s) 1105, 2001
BamHI GGATCC 1 cut(s) 1275
BanI GGYRCC 1 cut(s) 1702
BanII GRGCYC 1 cut(s) 271
BauI CACGAG 1 cut(s) 234
BbsI GAAGAC 1 cut(s) 1102
Bbv12I GWGCWC 3 cut(s) 271, 1472, 2319
BbvI GCAGC 8 cut(s) 469, 598, 715, 1009, 1117, 1403, 1522, 1544
BccI CCATC 5 cut(s) 569, 1954, 2065, 2069, 2158
BceAI ACGGC 2 cut(s) 656, 1365
BciT130I CCWGG 4 cut(s) 1210, 1457, 1665, 2254
BclI TGATCA 1 cut(s) 1501
BcnI CCSGG 1 cut(s) 2203
BcoDI GTCTC 1 cut(s) 2190
BfaI CTAG 3 cut(s) 1127, 1695, 2174
BfoI RGCGCY 1 cut(s) 1417
BfuAI ACCTGC 1 cut(s) 1743
BglII AGATCT 1 cut(s) 2108
BisI GCNGC 8 cut(s) 483, 612, 704, 1023, 1131, 1417, 1533, 1536
BlsI GCNGC 8 cut(s) 484, 613, 705, 1024, 1132, 1418, 1534, 1537
Bme1390I CCNGG 5 cut(s) 1210, 1457, 1665, 2203, 2254
Bme18I GGWCC 3 cut(s) 426, 544, 1660
BmgT120I GGNCC 5 cut(s) 426, 544, 1637, 1660, 1832
BmiI GGNNCC 7 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834
BmrFI CCNGG 5 cut(s) 1210, 1457, 1665, 2203, 2254
BmrI ACTGGG 1 cut(s) 1635
BmsI GCATC 1 cut(s) 524
BmtI GCTAGC 1 cut(s) 1698
BmuI ACTGGG 1 cut(s) 1635
BpiI GAAGAC 1 cut(s) 1102
BpmI CTGGAG 3 cut(s) 573, 1970, 2097
BpuEI CTTGAG 1 cut(s) 1050
BpuMI CCSGG 1 cut(s) 2203
Bsa29I ATCGAT 1 cut(s) 847
BsaAI YACGTR 1 cut(s) 2377
BsaHI GRCGYC 1 cut(s) 2199
BsaJI CCNNGG 9 cut(s) 84, 886, 1208, 1209, 1243, 1455, 1663, 1664, 2202
BsaWI WCCGGW 2 cut(s) 423, 1854
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 1462, 1646, 1670, 2063, 2120
Bse1I ACTGG 6 cut(s) 950, 1106, 1450, 1641, 1953, 2114
Bse21I CCTNAGG 1 cut(s) 252
Bse3DI GCAATG 2 cut(s) 377, 1293
BseBI CCWGG 4 cut(s) 1210, 1457, 1665, 2254
BseCI ATCGAT 1 cut(s) 847
BseDI CCNNGG 9 cut(s) 84, 886, 1208, 1209, 1243, 1455, 1663, 1664, 2202
BseGI GGATG 5 cut(s) 94, 262, 1987, 2057, 2173
BseLI CCNNNNNNNGG 6 cut(s) 85, 1462, 1646, 1670, 2063, 2120
BseMI GCAATG 2 cut(s) 377, 1293
BseMII CTCAG 4 cut(s) 243, 2001, 2151, 2156
BseNI ACTGG 6 cut(s) 950, 1106, 1450, 1641, 1953, 2114
BseRI GAGGAG 2 cut(s) 107, 127
BseSI GKGCMC 2 cut(s) 1472, 1707
BseXI GCAGC 8 cut(s) 469, 598, 715, 1009, 1117, 1403, 1522, 1544
BseYI CCCAGC 1 cut(s) 614
BshFI GGCC 6 cut(s) 1105, 1248, 1638, 1833, 1955, 2001
BshNI GGYRCC 1 cut(s) 1702
BshVI ATCGAT 1 cut(s) 847
BsiHKAI GWGCWC 3 cut(s) 271, 1472, 2319
BsiSI CCGG 3 cut(s) 424, 1855, 2202
BsiWI CGTACG 1 cut(s) 477
BslFI GGGAC 8 cut(s) 727, 928, 1360, 1438, 1646, 1781, 2081, 2321
BslI CCNNNNNNNGG 6 cut(s) 85, 1462, 1646, 1670, 2063, 2120
BsmAI GTCTC 1 cut(s) 2190
BsmBI CGTCTC 1 cut(s) 2190
BsmFI GGGAC 8 cut(s) 727, 928, 1360, 1438, 1646, 1781, 2081, 2321
BsmI GAATGC 2 cut(s) 161, 532
BsnI GGCC 6 cut(s) 1105, 1248, 1638, 1833, 1955, 2001
Bsp1286I GDGCHC 4 cut(s) 271, 1472, 1707, 2319
Bsp1407I TGTACA 3 cut(s) 117, 961, 1222
Bsp143I GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 3 cut(s) 348, 1352, 1698
BspANI GGCC 6 cut(s) 1105, 1248, 1638, 1833, 1955, 2001
BspCNI CTCAG 4 cut(s) 244, 2002, 2152, 2155
BspDI ATCGAT 1 cut(s) 847
BspHI TCATGA 4 cut(s) 466, 1042, 1398, 2341
BspLI GGNNCC 7 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834
BspMI ACCTGC 1 cut(s) 1743
BspOI GCTAGC 1 cut(s) 1698
BspPI GGATC 6 cut(s) 72, 242, 629, 1270, 1283, 1579
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 1702
BsrDI GCAATG 2 cut(s) 377, 1293
BsrGI TGTACA 3 cut(s) 117, 961, 1222
BsrI ACTGG 6 cut(s) 950, 1106, 1450, 1641, 1953, 2114
BssECI CCNNGG 9 cut(s) 84, 886, 1208, 1209, 1243, 1455, 1663, 1664, 2202
BssMI GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
BssNAI GTATAC 2 cut(s) 1683, 1791
BssNI GRCGYC 1 cut(s) 2199
BssSI CACGAG 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 84, 886
Bst1107I GTATAC 2 cut(s) 1683, 1791
Bst2BI CACGAG 1 cut(s) 234
Bst2UI CCWGG 4 cut(s) 1210, 1457, 1665, 2254
Bst6I CTCTTC 2 cut(s) 429, 1496
BstACI GRCGYC 1 cut(s) 2199
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 3 cut(s) 117, 961, 1222
BstBAI YACGTR 1 cut(s) 2377
BstDEI CTNAG 8 cut(s) 107, 252, 639, 902, 2010, 2142, 2160, 2297
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 1 cut(s) 1372
BstF5I GGATG 5 cut(s) 94, 262, 1987, 2057, 2173
BstH2I RGCGCY 1 cut(s) 1417
BstHHI GCGC 1 cut(s) 1416
BstKTI GATC 8 cut(s) 67, 250, 624, 1278, 1504, 1574, 2111, 2328
BstMAI GTCTC 1 cut(s) 2190
BstMBI GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
BstMWI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
BstNI CCWGG 4 cut(s) 1210, 1457, 1665, 2254
BstNSI RCATGY 4 cut(s) 395, 515, 703, 1852
BstPI GGTNACC 1 cut(s) 1372
BstSCI CCNGG 5 cut(s) 1208, 1455, 1663, 2201, 2252
BstSLI GKGCMC 2 cut(s) 1472, 1707
BstV1I GCAGC 8 cut(s) 469, 598, 715, 1009, 1117, 1403, 1522, 1544
BstV2I GAAGAC 1 cut(s) 1102
BstX2I RGATCY 4 cut(s) 247, 1275, 1571, 2108
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 4 cut(s) 247, 1275, 1571, 2108
BstZ17I GTATAC 2 cut(s) 1683, 1791
Bsu15I ATCGAT 1 cut(s) 847
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 6 cut(s) 1105, 1248, 1638, 1833, 1955, 2001
BsuTUI ATCGAT 1 cut(s) 847
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 5 cut(s) 94, 262, 1987, 2057, 2173
BtsIMutI CAGTG 4 cut(s) 1443, 2091, 2121, 2326
BveI ACCTGC 1 cut(s) 1743
CaiI CAGNNNCTG 2 cut(s) 251, 614
CciI TCATGA 4 cut(s) 466, 1042, 1398, 2341
CfoI GCGC 1 cut(s) 1416
Cfr13I GGNCC 5 cut(s) 426, 544, 1637, 1660, 1832
ClaI ATCGAT 1 cut(s) 847
CpoI CGGWCCG 1 cut(s) 426
CseI GACGC 1 cut(s) 2207
Csp6I GTAC 8 cut(s) 118, 316, 478, 962, 1037, 1223, 1487, 1987
CspI CGGWCCG 1 cut(s) 426
CviQI GTAC 8 cut(s) 118, 316, 478, 962, 1037, 1223, 1487, 1987
DdeI CTNAG 8 cut(s) 107, 252, 639, 902, 2010, 2142, 2160, 2297
DpnI GATC 8 cut(s) 66, 249, 623, 1277, 1503, 1573, 2110, 2327
DpnII GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
DraI TTTAAA 1 cut(s) 856
DraIII CACNNNGTG 1 cut(s) 2323
EaeI YGGCCR 3 cut(s) 1103, 1246, 1999
Eam1104I CTCTTC 2 cut(s) 429, 1496
EarI CTCTTC 2 cut(s) 429, 1496
Ecl136II GAGCTC 1 cut(s) 269
Eco130I CCWWGG 2 cut(s) 84, 886
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 3 cut(s) 426, 544, 1660
Eco53kI GAGCTC 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 252
Eco91I GGTNACC 1 cut(s) 1372
EcoICRI GAGCTC 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 1660
EcoO65I GGTNACC 1 cut(s) 1372
EcoRII CCWGG 4 cut(s) 1208, 1455, 1663, 2252
EcoT14I CCWWGG 2 cut(s) 84, 886
EcoT22I ATGCAT 2 cut(s) 517, 2293
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 2 cut(s) 84, 886
Esp3I CGTCTC 1 cut(s) 2190
FaqI GGGAC 8 cut(s) 727, 928, 1360, 1438, 1646, 1781, 2081, 2321
FauI CCCGC 2 cut(s) 341, 1691
FbaI TGATCA 1 cut(s) 1501
FblI GTMKAC 3 cut(s) 1682, 1790, 2081
Fnu4HI GCNGC 8 cut(s) 483, 612, 704, 1023, 1131, 1417, 1533, 1536
FokI GGATG 5 cut(s) 101, 269, 1994, 2044, 2180
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 8 cut(s) 483, 612, 704, 1023, 1131, 1417, 1533, 1536
FspBI CTAG 3 cut(s) 1127, 1695, 2174
GlaI GCGC 1 cut(s) 1415
GluI GCNGC 8 cut(s) 483, 612, 704, 1023, 1131, 1417, 1533, 1536
GsaI CCCAGC 1 cut(s) 618
GsuI CTGGAG 3 cut(s) 573, 1970, 2097
HaeII RGCGCY 1 cut(s) 1417
HaeIII GGCC 6 cut(s) 1105, 1248, 1638, 1833, 1955, 2001
HapII CCGG 3 cut(s) 424, 1855, 2202
HgaI GACGC 1 cut(s) 2207
HhaI GCGC 1 cut(s) 1416
Hin1I GRCGYC 1 cut(s) 2199
Hin6I GCGC 1 cut(s) 1414
HinP1I GCGC 1 cut(s) 1414
HincII GTYRAC 1 cut(s) 2082
HindII GTYRAC 1 cut(s) 2082
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 3 cut(s) 424, 1855, 2202
HphI GGTGA 2 cut(s) 822, 1720
Hpy166II GTNNAC 5 cut(s) 631, 1470, 1683, 1791, 2082
Hpy188I TCNGA 5 cut(s) 69, 247, 1975, 1981, 2145
Hpy8I GTNNAC 5 cut(s) 631, 1470, 1683, 1791, 2082
HpyAV CCTTC 3 cut(s) 500, 1007, 1958
HpyCH4IV ACGT 2 cut(s) 1582, 2376
HpyF10VI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
HpyF3I CTNAG 8 cut(s) 107, 252, 639, 902, 2010, 2142, 2160, 2297
HpySE526I ACGT 2 cut(s) 1582, 2376
Hsp92I GRCGYC 1 cut(s) 2199
HspAI GCGC 1 cut(s) 1414
KflI GGGWCCC 1 cut(s) 1660
Ksp22I TGATCA 1 cut(s) 1501
Kzo9I GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 266, 745, 2314
Lsp1109I GCAGC 8 cut(s) 469, 598, 715, 1009, 1117, 1403, 1522, 1544
LweI GCATC 1 cut(s) 524
MaeI CTAG 3 cut(s) 1127, 1695, 2174
MaeII ACGT 2 cut(s) 1582, 2376
MaeIII GTNAC 5 cut(s) 1060, 1300, 1372, 1874, 2134
MalI GATC 8 cut(s) 66, 249, 623, 1277, 1503, 1573, 2110, 2327
MboI GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
MfeI CAATTG 2 cut(s) 744, 2188
MflI RGATCY 4 cut(s) 247, 1275, 1571, 2108
MhlI GDGCHC 4 cut(s) 271, 1472, 1707, 2319
MlsI TGGCCA 2 cut(s) 1105, 2001
MluNI TGGCCA 2 cut(s) 1105, 2001
MlyI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2356
MmeI TCCRAC 5 cut(s) 244, 483, 857, 1651, 2341
Mox20I TGGCCA 2 cut(s) 1105, 2001
Mph1103I ATGCAT 2 cut(s) 517, 2293
MroXI GAANNNNTTC 1 cut(s) 2050
MscI TGGCCA 2 cut(s) 1105, 2001
MseI TTAA 5 cut(s) 855, 1179, 1215, 1269, 2033
MslI CAYNNNNRTG 3 cut(s) 560, 983, 1298
Msp20I TGGCCA 2 cut(s) 1105, 2001
MspA1I CMGCKG 4 cut(s) 348, 611, 959, 1535
MspI CCGG 3 cut(s) 424, 1855, 2202
MspR9I CCNGG 5 cut(s) 1210, 1457, 1665, 2203, 2254
MunI CAATTG 2 cut(s) 744, 2188
Mva1269I GAATGC 2 cut(s) 161, 532
MvaI CCWGG 4 cut(s) 1210, 1457, 1665, 2254
MwoI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
NciI CCSGG 1 cut(s) 2203
NcoI CCATGG 1 cut(s) 84
NdeII GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
NheI GCTAGC 1 cut(s) 1694
NlaIV GGNNCC 7 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834
NmeAIII GCCGAG 1 cut(s) 1224
NmuCI GTSAC 3 cut(s) 1060, 1300, 2134
NsiI ATGCAT 2 cut(s) 517, 2293
NspI RCATGY 4 cut(s) 395, 515, 703, 1852
OliI CACNNNNGTG 1 cut(s) 983
PaeI GCATGC 3 cut(s) 395, 515, 703
PagI TCATGA 4 cut(s) 466, 1042, 1398, 2341
PasI CCCWGGG 2 cut(s) 1209, 1664
PciI ACATGT 1 cut(s) 1848
PciSI GCTCTTC 1 cut(s) 429
PctI GAATGC 2 cut(s) 161, 532
PdmI GAANNNNTTC 1 cut(s) 2050
PfeI GAWTC 7 cut(s) 580, 1285, 1311, 1426, 1812, 2090, 2249
Pfl23II CGTACG 1 cut(s) 477
PflMI CCANNNNNTGG 2 cut(s) 85, 2120
PkrI GCNGC 8 cut(s) 484, 613, 705, 1024, 1132, 1418, 1534, 1537
PleI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2355
PpsI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2355
Ppu21I YACGTR 1 cut(s) 2377
PpuMI RGGWCCY 1 cut(s) 1660
PscI ACATGT 1 cut(s) 1848
PshBI ATTAAT 1 cut(s) 1269
Psp124BI GAGCTC 1 cut(s) 271
Psp1406I AACGTT 1 cut(s) 1582
Psp5II RGGWCCY 1 cut(s) 1660
Psp6I CCWGG 4 cut(s) 1208, 1455, 1663, 2252
PspEI GGTNACC 1 cut(s) 1372
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 4 cut(s) 1208, 1455, 1663, 2252
PspLI CGTACG 1 cut(s) 477
PspN4I GGNNCC 7 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834
PspPI GGNCC 5 cut(s) 426, 544, 1637, 1660, 1832
PspPPI RGGWCCY 1 cut(s) 1660
PstNI CAGNNNCTG 2 cut(s) 251, 614
PsuI RGATCY 4 cut(s) 247, 1275, 1571, 2108
PvuII CAGCTG 3 cut(s) 611, 959, 1535
RsaI GTAC 8 cut(s) 119, 317, 479, 963, 1038, 1224, 1488, 1988
RsaNI GTAC 8 cut(s) 118, 316, 478, 962, 1037, 1223, 1487, 1987
RseI CAYNNNNRTG 3 cut(s) 560, 983, 1298
Rsr2I CGGWCCG 1 cut(s) 426
RsrII CGGWCCG 1 cut(s) 426
SacI GAGCTC 1 cut(s) 271
SalI GTCGAC 1 cut(s) 2080
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 5 cut(s) 855, 1179, 1215, 1269, 2033
SatI GCNGC 8 cut(s) 483, 612, 704, 1023, 1131, 1417, 1533, 1536
Sau3AI GATC 8 cut(s) 64, 247, 621, 1275, 1501, 1571, 2108, 2325
Sau96I GGNCC 5 cut(s) 426, 544, 1637, 1660, 1832
SchI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2356
ScrFI CCNGG 5 cut(s) 1210, 1457, 1665, 2203, 2254
SduI GDGCHC 4 cut(s) 271, 1472, 1707, 2319
SfaNI GCATC 1 cut(s) 524
SinI GGWCC 3 cut(s) 426, 544, 1660
SmiMI CAYNNNNRTG 3 cut(s) 560, 983, 1298
SmlI CTYRAG 1 cut(s) 1065
SmoI CTYRAG 1 cut(s) 1065
SphI GCATGC 3 cut(s) 395, 515, 703
SsiI CCGC 3 cut(s) 348, 1352, 1698
SspMI CTAG 3 cut(s) 1127, 1695, 2174
SstI GAGCTC 1 cut(s) 271
StyD4I CCNGG 5 cut(s) 1208, 1455, 1663, 2201, 2252
StyI CCWWGG 2 cut(s) 84, 886
TaiI ACGT 2 cut(s) 1585, 2379
TaqI TCGA 4 cut(s) 847, 1945, 2018, 2081
TaqII GACCGA 1 cut(s) 443
TatI WGTACW 7 cut(s) 117, 315, 961, 1036, 1222, 1486, 1986
TfiI GAWTC 7 cut(s) 580, 1285, 1311, 1426, 1812, 2090, 2249
Tru1I TTAA 5 cut(s) 855, 1179, 1215, 1269, 2033
Tru9I TTAA 5 cut(s) 855, 1179, 1215, 1269, 2033
TscAI CASTG 4 cut(s) 1450, 2091, 2121, 2326
TseFI GTSAC 3 cut(s) 1060, 1300, 2134
TseI GCWGC 8 cut(s) 482, 611, 703, 1022, 1130, 1416, 1532, 1535
Tsp45I GTSAC 3 cut(s) 1060, 1300, 2134
TspRI CASTG 4 cut(s) 1450, 2091, 2121, 2326
Van91I CCANNNNNTGG 2 cut(s) 85, 2120
VneI GTGCAC 1 cut(s) 1468
VpaK11BI GGWCC 3 cut(s) 426, 544, 1660
VspI ATTAAT 1 cut(s) 1269
XapI RAATTY 5 cut(s) 4, 240, 1840, 2177, 2367
XceI RCATGY 4 cut(s) 395, 515, 703, 1852
XcmI CCANNNNNNNNNTGG 3 cut(s) 1269, 1648, 2083
XmiI GTMKAC 3 cut(s) 1682, 1790, 2081
XmnI GAANNNNTTC 1 cut(s) 2050
XspI CTAG 3 cut(s) 1127, 1695, 2174
Zsp2I ATGCAT 2 cut(s) 517, 2293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.