Rorug05G0060100

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000477
Physical Location & Seq
Forward (+)
5244711 .. 5251454
6744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0060100.1

Sequence Viewer

Length: 2199 bp
ATGGCTCCGACGACGATGATCGTCACCAACACTCCGGCCGCCGACCTCGCTCTCACCAACCTCGCCTACTGCTCGCCGCTCGATCTCCAGACCTTCGCCATTCCCGGCACCAAGCTCAACCTAGCTCTAATCGCCGACTCGTTCGTCCTCTCAGTCTGTTCTCACCAGAGCATACAGAAGGGTTACATTGCGCTGAATGCGATTCAACGACGGCATGCTAGGGTTTCTAGCGGTGACTCATTATCTGTGAGCAGGTTTACTCCGCCTGATGATTTCAACCTGACCGTGCTCACTCTGGAATTGGAGTTTGTGAAAAAAGGGACTAAGAGTGAACAGATTGATGCTGTTGCCTTGTCGAGCCAACTTCAAAAGAGGTTTATCAACCAGGTTATGACAGCCGGACAAAGAGTATCATTTGAGTACCTTGGGACTAATTACATTTTTACAGTCAATCAAGCTGTAAGATCCGAAGAGGCTAGCTCTGTTGAAAGAGGGATGATCTCGAGTGAAACATACTTCATTTTTGAGGCACCGAGTGGAAATGGAATAAAGATTGTGAACCAACGTTCTGCTACGACTAGCAATATCTTTAAGCACAAGGAATTCAACCTTCAATCACTTGGCATTGGTGGCCTAGGTGCTGAGTTTGCAGATATATTTCGAAGAGCTTTTGCCTCCCGTGTCTTCCCTGCACATGTTACAAATAAACTAGGGATTAAGCATGTGAAGGGTATGCTGCTTTATGGACCACCTGGTACTGGAAAAACTCTTATGGCGCGTCAAATTGGAAAAATGTTGAATGGGAAAGATCCAAAGATTGTTAATGGTCCAGAAGTCCTGAGCAAGTTCGTTGGTGAAACTGAAAAAAATGTTAGGGATCTATTTGCTGATGCTGAGAATGATCAAAGGCTTCGTGGAGATGAAAGTGAATTGCATGTCATAATCTTTGATGAAATCGATGCTATTTGTAAGTCAAGAGGATCAACTAGAGATGGCACAGGAGTTCATGATAGTATTGTGAACCAGTTACTTACAAAGATTGATGGTGTGGAATCTCTGAATAATGTTTTGCTTATTGGGATGACCAACAGAAAGGATTTGATTGATGAAGCCCTTTTGAGGCCAGGACGTCTGGAAGTTCAAATTGAGATAAGTCTTCCTGATGAAAATGGTCGCATACAGATTCTTCAAATTCATACGAACAAAATGAAGGAAAGCTCTTTTCTTGCTCCTGATGTCAACCTGCAAGAGCTTGCTGCTCGTACAAAGAACTATAGTGGTGCAGAACTGGAAGGTGTTGTTAAAAGTGCAGTATCATTTGCTTTGAATAGACAACTAAGTTTGGAGGATCTTACCAAGCCAGTGGATGAAGAGAGTATTAAGGTTACCATGGATGATTTTCTACGTGCACTCCATGAAATTGTACCTGCTTTTGGTGCCTCCACTGATGACCTTGAAAGATGCAGACTTCATGGCATGGTGGATTGTGGGGATCGACATAAGCATATCTATCAAAGAGCCATGCTACTTGTGGAACAAGTTAAATCAAGTGAGAGAAGTCCTCTTGTTACATGCCTTTTGGAAGGTCCTAGTGGCAGTGGTAAATCTTCACTAGCAGCTACTGTTGGCATCGACAGTGATTTCCCCTATGTTAAGATTATCTCGGCTGAGGCCATGATTGGTCTTCAAGAGAGCACCAAATGTGCGCAAATCGCAAAGGTTTTTGAAGATGCATACAAGTCACCATTAAGCATTATCATCCTGGACAATATTGAAAGGTTACTGGAGTATATTGCTATTGGTCCGAGGTTTTCAAATTTGATATCTCAAACACTGATGCTCCTTCTCAAGCGCACTCCTCCAAAGGGGAAAAAGCTTCTGGTTTTTGGTACAACAAGTGAATTGGGCTTCTTAGACTCGATTGGTTTTTGTGATACCTTCTCCGTCACTTACAATGTTCCCACATTACAGACACCGGCTGCAAAGAAGGTGCTAGAACAGTTAAATGTCTTTGCTGATGAAGACATAGATGCAGCTGCAGAGGCCCTGAATGATATTCCTATCAAGAAGCTCTATATGGTAATTGAGATGGCAGCCCAGGGTGAGCAAGGAGGGTCTGCCCAGGCAATCTTCTCTGGACAAGCGAAAATAGAGATCAATCACTTTTATGCGATCCTTCAGGATACTGTTAGATATTAG

Protein Analysis

732

Amino Acids

80.33

Weight (kDa)

5.76

Isoelectric Point (pI)

37.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CDC48_2 PF02933 112 - 156 5.8e-06 Cell division protein 48 (CDC48), domain 2
AAA PF00004 245 - 385 2.5e-36 ATPase family associated with various cellular activities (AAA)
AAA_lid_3 PF17862 412 - 447 3.4e-10 AAA+ lid domain
AAA PF00004 526 - 639 2.9e-08 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 143
AatII GACGTC 1 cut(s) 1132
Acc16I TGCGCA 1 cut(s) 1707
Acc36I ACCTGC 3 cut(s) 243, 1251, 1435
AccB1I GGYRCC 3 cut(s) 107, 529, 1436
AccBSI CCGCTC 1 cut(s) 79
AccII CGCG 1 cut(s) 778
AciI CCGC 4 cut(s) 39, 77, 231, 263
AclI AACGTT 1 cut(s) 565
AclWI GGATC 7 cut(s) 459, 803, 885, 988, 1356, 1500, 2167
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 3 cut(s) 602, 1191, 1816
AcuI CTGAAG 1 cut(s) 2162
AcyI GRCGYC 1 cut(s) 1129
AdeI CACNNNGTG 1 cut(s) 536
AfaI GTAC 5 cut(s) 422, 757, 1264, 1425, 1891
AfiI CCNNNNNNNGG 4 cut(s) 758, 1119, 1433, 1865
AflIII ACRYGT 1 cut(s) 694
AjnI CCWGG 6 cut(s) 384, 751, 1123, 1761, 2097, 2121
Alw21I GWGCWC 3 cut(s) 291, 1411, 1697
Alw44I GTGCAC 1 cut(s) 1407
AlwI GGATC 7 cut(s) 459, 803, 885, 988, 1356, 1500, 2167
AlwNI CAGNNNCTG 1 cut(s) 1622
Ama87I CYCGRG 1 cut(s) 502
AoxI GGCC 5 cut(s) 36, 631, 1121, 1671, 2043
ApaLI GTGCAC 1 cut(s) 1407
ApeKI GCWGC 7 cut(s) 736, 1256, 1616, 1979, 2033, 2036, 2093
ApoI RAATTY 3 cut(s) 602, 1191, 1816
AspA2I CCTAGG 1 cut(s) 634
AspLEI GCGC 4 cut(s) 193, 778, 1708, 1854
AspS9I GGNCC 5 cut(s) 746, 827, 1586, 1802, 2044
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 7 cut(s) 16, 46, 155, 245, 866, 1734, 2114
AsuII TTCGAA 1 cut(s) 661
AsuNHI GCTAGC 1 cut(s) 476
AvaI CYCGRG 1 cut(s) 502
AvaII GGWCC 4 cut(s) 746, 827, 1586, 1802
AvrII CCTAGG 1 cut(s) 634
BaeGI GKGCMC 1 cut(s) 1411
BanI GGYRCC 3 cut(s) 107, 529, 1436
BbsI GAAGAC 4 cut(s) 676, 1148, 1676, 2028
Bbv12I GWGCWC 3 cut(s) 291, 1411, 1697
BbvCI CCTCAGC 1 cut(s) 1668
BbvI GCAGC 7 cut(s) 723, 1243, 1628, 1966, 2023, 2045, 2105
BccI CCATC 3 cut(s) 986, 1037, 2083
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 6 cut(s) 386, 753, 1125, 1763, 2099, 2123
BciVI GTATCC 1 cut(s) 2176
BclI TGATCA 1 cut(s) 901
BcnI CCSGG 1 cut(s) 105
BfmI CTRYAG 2 cut(s) 1273, 2037
BfuAI ACCTGC 3 cut(s) 243, 1251, 1435
BfuI GTATCC 1 cut(s) 2176
BisI GCNGC 9 cut(s) 39, 77, 737, 1257, 1617, 1980, 2034, 2037, 2094
BlnI CCTAGG 1 cut(s) 634
BlsI GCNGC 9 cut(s) 40, 78, 738, 1258, 1618, 1981, 2035, 2038, 2095
Bme1390I CCNGG 7 cut(s) 105, 386, 753, 1125, 1763, 2099, 2123
Bme18I GGWCC 4 cut(s) 746, 827, 1586, 1802
BmeT110I CYCGRG 1 cut(s) 502
BmgT120I GGNCC 5 cut(s) 746, 827, 1586, 1802, 2044
BmiI GGNNCC 4 cut(s) 6, 109, 531, 1438
BmrFI CCNGG 7 cut(s) 105, 386, 753, 1125, 1763, 2099, 2123
BmsI GCATC 8 cut(s) 331, 880, 949, 1451, 1638, 1720, 1827, 2020
BmtI GCTAGC 1 cut(s) 480
BpiI GAAGAC 4 cut(s) 676, 1148, 1676, 2028
BplI GAGNNNNNCTC 4 cut(s) 464, 496, 1546, 1578
BpmI CTGGAG 2 cut(s) 71, 1805
Bpu10I CCTNAGC 2 cut(s) 839, 1668
Bpu14I TTCGAA 1 cut(s) 661
BpuEI CTTGAG 1 cut(s) 1832
BpuMI CCSGG 1 cut(s) 105
Bsa29I ATCGAT 1 cut(s) 957
BsaAI YACGTR 1 cut(s) 1406
BsaHI GRCGYC 1 cut(s) 1129
BsaJI CCNNGG 7 cut(s) 424, 634, 1389, 1805, 2097, 2098, 2121
BsaXI ACNNNNNCTCC 6 cut(s) 16, 46, 1395, 1425, 1824, 1854
Bsc4I CCNNNNNNNGG 4 cut(s) 758, 1119, 1433, 1865
Bse118I RCCGGY 1 cut(s) 1975
Bse1I ACTGG 5 cut(s) 763, 1024, 1293, 1361, 1788
Bse3DI GCAATG 1 cut(s) 186
BseBI CCWGG 6 cut(s) 386, 753, 1125, 1763, 2099, 2123
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 7 cut(s) 424, 634, 1389, 1805, 2097, 2098, 2121
BseGI GGATG 5 cut(s) 501, 1086, 1372, 1399, 1758
BseLI CCNNNNNNNGG 4 cut(s) 758, 1119, 1433, 1865
BseMI GCAATG 1 cut(s) 186
BseMII CTCAG 5 cut(s) 165, 633, 830, 885, 1659
BseNI ACTGG 5 cut(s) 763, 1024, 1293, 1361, 1788
BseRI GAGGAG 1 cut(s) 1848
BseSI GKGCMC 1 cut(s) 1411
BseX3I CGGCCG 1 cut(s) 36
BseXI GCAGC 7 cut(s) 723, 1243, 1628, 1966, 2023, 2045, 2105
BsgI GTGCAG 3 cut(s) 675, 1302, 1329
Bsh1236I CGCG 1 cut(s) 778
Bsh1285I CGRYCG 1 cut(s) 39
BshFI GGCC 5 cut(s) 38, 633, 1123, 1673, 2045
BshNI GGYRCC 3 cut(s) 107, 529, 1436
BshVI ATCGAT 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 39
BsiHKAI GWGCWC 3 cut(s) 291, 1411, 1697
BsiHKCI CYCGRG 1 cut(s) 502
BsiSI CCGG 4 cut(s) 35, 105, 399, 1976
BslFI GGGAC 2 cut(s) 334, 442
BslI CCNNNNNNNGG 4 cut(s) 758, 1119, 1433, 1865
BsmFI GGGAC 2 cut(s) 334, 442
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 5 cut(s) 38, 633, 1123, 1673, 2045
BsoBI CYCGRG 1 cut(s) 502
Bsp119I TTCGAA 1 cut(s) 661
Bsp1286I GDGCHC 3 cut(s) 291, 1411, 1697
Bsp19I CCATGG 1 cut(s) 1389
BspACI CCGC 4 cut(s) 39, 77, 231, 263
BspANI GGCC 5 cut(s) 38, 633, 1123, 1673, 2045
BspCNI CTCAG 5 cut(s) 164, 634, 831, 886, 1660
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 778
BspHI TCATGA 1 cut(s) 1006
BspLI GGNNCC 4 cut(s) 6, 109, 531, 1438
BspMAI CTGCAG 1 cut(s) 2041
BspMI ACCTGC 3 cut(s) 243, 1251, 1435
BspOI GCTAGC 1 cut(s) 480
BspPI GGATC 7 cut(s) 459, 803, 885, 988, 1356, 1500, 2167
BspQI GCTCTTC 1 cut(s) 658
BspT104I TTCGAA 1 cut(s) 661
BspT107I GGYRCC 3 cut(s) 107, 529, 1436
BsrBI CCGCTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 186
BsrFI RCCGGY 1 cut(s) 1975
BsrI ACTGG 5 cut(s) 763, 1024, 1293, 1361, 1788
BssAI RCCGGY 1 cut(s) 1975
BssECI CCNNGG 7 cut(s) 424, 634, 1389, 1805, 2097, 2098, 2121
BssNI GRCGYC 1 cut(s) 1129
BssT1I CCWWGG 3 cut(s) 424, 634, 1389
Bst2UI CCWGG 6 cut(s) 386, 753, 1125, 1763, 2099, 2123
Bst4CI ACNGT 6 cut(s) 286, 448, 1624, 1637, 2001, 2188
Bst6I CTCTTC 3 cut(s) 465, 658, 1365
BstACI GRCGYC 1 cut(s) 1129
BstBAI YACGTR 1 cut(s) 1406
BstBI TTCGAA 1 cut(s) 661
BstC8I GCNNGC 4 cut(s) 74, 216, 478, 1254
BstDEI CTNAG 8 cut(s) 151, 324, 642, 839, 894, 1337, 1668, 1912
BstDSI CCRYGG 1 cut(s) 1389
BstEII GGTNACC 1 cut(s) 1384
BstF5I GGATG 5 cut(s) 501, 1086, 1372, 1399, 1758
BstFNI CGCG 1 cut(s) 778
BstHHI GCGC 4 cut(s) 193, 778, 1708, 1854
BstMCI CGRYCG 1 cut(s) 39
BstMWI GCNNNNNNNGC 8 cut(s) 47, 131, 197, 630, 647, 1436, 1712, 2042
BstNI CCWGG 6 cut(s) 386, 753, 1125, 1763, 2099, 2123
BstNSI RCATGY 5 cut(s) 218, 698, 725, 938, 1575
BstPI GGTNACC 1 cut(s) 1384
BstSCI CCNGG 7 cut(s) 103, 384, 751, 1123, 1761, 2097, 2121
BstSFI CTRYAG 2 cut(s) 1273, 2037
BstSLI GKGCMC 1 cut(s) 1411
BstUI CGCG 1 cut(s) 778
BstV1I GCAGC 7 cut(s) 723, 1243, 1628, 1966, 2023, 2045, 2105
BstV2I GAAGAC 4 cut(s) 676, 1148, 1676, 2028
BstX2I RGATCY 4 cut(s) 464, 808, 877, 1348
BstXI CCANNNNNNTGG 1 cut(s) 1363
BstYI RGATCY 4 cut(s) 464, 808, 877, 1348
BstZI CGGCCG 1 cut(s) 36
Bsu15I ATCGAT 1 cut(s) 957
BsuI GTATCC 1 cut(s) 2176
BsuRI GGCC 5 cut(s) 38, 633, 1123, 1673, 2045
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 1389
BtsCI GGATG 5 cut(s) 501, 1086, 1372, 1399, 1758
BtsI GCAGTG 1 cut(s) 1603
BtsIMutI CAGTG 5 cut(s) 1368, 1443, 1603, 1642, 1832
BveI ACCTGC 3 cut(s) 243, 1251, 1435
Cac8I GCNNGC 4 cut(s) 74, 216, 478, 1254
CaiI CAGNNNCTG 1 cut(s) 1622
CciI TCATGA 1 cut(s) 1006
CfoI GCGC 4 cut(s) 193, 778, 1708, 1854
Cfr10I RCCGGY 1 cut(s) 1975
Cfr13I GGNCC 5 cut(s) 746, 827, 1586, 1802, 2044
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 767
CsiI ACCWGGT 2 cut(s) 384, 751
Csp6I GTAC 5 cut(s) 421, 756, 1263, 1424, 1890
CviQI GTAC 5 cut(s) 421, 756, 1263, 1424, 1890
DdeI CTNAG 8 cut(s) 151, 324, 642, 839, 894, 1337, 1668, 1912
DraIII CACNNNGTG 1 cut(s) 536
DrdI GACNNNNNNGTC 1 cut(s) 143
DseDI GACNNNNNNGTC 1 cut(s) 143
EaeI YGGCCR 1 cut(s) 36
EagI CGGCCG 1 cut(s) 36
Eam1104I CTCTTC 3 cut(s) 465, 658, 1365
EarI CTCTTC 3 cut(s) 465, 658, 1365
EciI GGCGGA 1 cut(s) 252
EclXI CGGCCG 1 cut(s) 36
Eco130I CCWWGG 3 cut(s) 424, 634, 1389
Eco32I GATATC 1 cut(s) 1824
Eco47I GGWCC 4 cut(s) 746, 827, 1586, 1802
Eco52I CGGCCG 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 2162
Eco88I CYCGRG 1 cut(s) 502
Eco91I GGTNACC 1 cut(s) 1384
EcoO109I RGGNCCY 2 cut(s) 1586, 2044
EcoO65I GGTNACC 1 cut(s) 1384
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 6 cut(s) 384, 751, 1123, 1761, 2097, 2121
EcoRV GATATC 1 cut(s) 1824
EcoT14I CCWWGG 3 cut(s) 424, 634, 1389
EcoT22I ATGCAT 1 cut(s) 1735
ErhI CCWWGG 3 cut(s) 424, 634, 1389
FaqI GGGAC 2 cut(s) 334, 442
FbaI TGATCA 1 cut(s) 901
Fnu4HI GCNGC 9 cut(s) 39, 77, 737, 1257, 1617, 1980, 2034, 2037, 2094
FokI GGATG 5 cut(s) 508, 1093, 1379, 1406, 1745
Fsp4HI GCNGC 9 cut(s) 39, 77, 737, 1257, 1617, 1980, 2034, 2037, 2094
FspI TGCGCA 1 cut(s) 1707
GlaI GCGC 4 cut(s) 192, 777, 1707, 1853
GluI GCNGC 9 cut(s) 39, 77, 737, 1257, 1617, 1980, 2034, 2037, 2094
GsuI CTGGAG 2 cut(s) 71, 1805
HaeIII GGCC 5 cut(s) 38, 633, 1123, 1673, 2045
HapII CCGG 4 cut(s) 35, 105, 399, 1976
HgaI GACGC 1 cut(s) 767
HhaI GCGC 4 cut(s) 193, 778, 1708, 1854
Hin1I GRCGYC 1 cut(s) 1129
Hin6I GCGC 4 cut(s) 191, 776, 1706, 1852
HinP1I GCGC 4 cut(s) 191, 776, 1706, 1852
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HindIII AAGCTT 1 cut(s) 1874
HinfI GANTC 6 cut(s) 137, 202, 236, 1052, 1183, 1916
HpaII CCGG 4 cut(s) 35, 105, 399, 1976
HphI GGTGA 7 cut(s) 16, 46, 155, 245, 866, 1734, 2114
Hpy166II GTNNAC 6 cut(s) 258, 332, 559, 1021, 1240, 1409
Hpy188I TCNGA 4 cut(s) 9, 469, 1059, 1806
Hpy8I GTNNAC 6 cut(s) 258, 332, 559, 1021, 1240, 1409
Hpy99I CGWCG 3 cut(s) 13, 16, 213
HpyCH4III ACNGT 6 cut(s) 286, 448, 1624, 1637, 2001, 2188
HpyCH4IV ACGT 3 cut(s) 565, 1129, 1405
HpyF10VI GCNNNNNNNGC 8 cut(s) 47, 131, 197, 630, 647, 1436, 1712, 2042
HpyF3I CTNAG 8 cut(s) 151, 324, 642, 839, 894, 1337, 1668, 1912
HpySE526I ACGT 3 cut(s) 565, 1129, 1405
Hsp92I GRCGYC 1 cut(s) 1129
HspAI GCGC 4 cut(s) 191, 776, 1706, 1852
Ksp22I TGATCA 1 cut(s) 901
LguI GCTCTTC 1 cut(s) 658
LmnI GCTCC 3 cut(s) 10, 1234, 1845
Lsp1109I GCAGC 7 cut(s) 723, 1243, 1628, 1966, 2023, 2045, 2105
LweI GCATC 8 cut(s) 331, 880, 949, 1451, 1638, 1720, 1827, 2020
MabI ACCWGGT 2 cut(s) 384, 751
MaeII ACGT 3 cut(s) 565, 1129, 1405
MbiI CCGCTC 1 cut(s) 79
MflI RGATCY 4 cut(s) 464, 808, 877, 1348
MhlI GDGCHC 3 cut(s) 291, 1411, 1697
MlyI GAGTC 3 cut(s) 131, 230, 1910
MmeI TCCRAC 1 cut(s) 32
Mph1103I ATGCAT 1 cut(s) 1735
MseI TTAA 9 cut(s) 591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
MslI CAYNNNNRTG 1 cut(s) 2166
MspA1I CMGCKG 1 cut(s) 2036
MspI CCGG 4 cut(s) 35, 105, 399, 1976
MspR9I CCNGG 7 cut(s) 105, 386, 753, 1125, 1763, 2099, 2123
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 6 cut(s) 386, 753, 1125, 1763, 2099, 2123
MvnI CGCG 1 cut(s) 778
MwoI GCNNNNNNNGC 8 cut(s) 47, 131, 197, 630, 647, 1436, 1712, 2042
NciI CCSGG 1 cut(s) 105
NcoI CCATGG 1 cut(s) 1389
NheI GCTAGC 1 cut(s) 476
NlaIV GGNNCC 4 cut(s) 6, 109, 531, 1438
NmeAIII GCCGAG 1 cut(s) 1643
NmuCI GTSAC 4 cut(s) 22, 233, 1740, 1945
NsbI TGCGCA 1 cut(s) 1707
NsiI ATGCAT 1 cut(s) 1735
NspI RCATGY 5 cut(s) 218, 698, 725, 938, 1575
NspV TTCGAA 1 cut(s) 661
PaeI GCATGC 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 502
PagI TCATGA 1 cut(s) 1006
PasI CCCWGGG 1 cut(s) 2098
PciI ACATGT 1 cut(s) 694
PciSI GCTCTTC 1 cut(s) 658
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 3 cut(s) 202, 1052, 1183
PfoI TCCNGGA 1 cut(s) 1761
PkrI GCNGC 9 cut(s) 40, 78, 738, 1258, 1618, 1981, 2035, 2038, 2095
PleI GAGTC 3 cut(s) 131, 230, 1910
PpsI GAGTC 3 cut(s) 131, 230, 1910
Ppu21I YACGTR 1 cut(s) 1406
PpuMI RGGWCCY 1 cut(s) 1586
PscI ACATGT 1 cut(s) 694
Psp1406I AACGTT 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 1586
Psp6I CCWGG 6 cut(s) 384, 751, 1123, 1761, 2097, 2121
PspEI GGTNACC 1 cut(s) 1384
PspGI CCWGG 6 cut(s) 384, 751, 1123, 1761, 2097, 2121
PspN4I GGNNCC 4 cut(s) 6, 109, 531, 1438
PspPI GGNCC 5 cut(s) 746, 827, 1586, 1802, 2044
PspPPI RGGWCCY 1 cut(s) 1586
PstI CTGCAG 1 cut(s) 2041
PstNI CAGNNNCTG 1 cut(s) 1622
PsuI RGATCY 4 cut(s) 464, 808, 877, 1348
PvuII CAGCTG 1 cut(s) 2036
RsaI GTAC 5 cut(s) 422, 757, 1264, 1425, 1891
RsaNI GTAC 5 cut(s) 421, 756, 1263, 1424, 1890
RseI CAYNNNNRTG 1 cut(s) 2166
SapI GCTCTTC 1 cut(s) 658
SaqAI TTAA 9 cut(s) 591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
SatI GCNGC 9 cut(s) 39, 77, 737, 1257, 1617, 1980, 2034, 2037, 2094
Sau96I GGNCC 5 cut(s) 746, 827, 1586, 1802, 2044
SchI GAGTC 3 cut(s) 131, 230, 1910
ScrFI CCNGG 7 cut(s) 105, 386, 753, 1125, 1763, 2099, 2123
SduI GDGCHC 3 cut(s) 291, 1411, 1697
SexAI ACCWGGT 2 cut(s) 384, 751
SfaNI GCATC 8 cut(s) 331, 880, 949, 1451, 1638, 1720, 1827, 2020
SfcI CTRYAG 2 cut(s) 1273, 2037
Sfr274I CTCGAG 1 cut(s) 502
SfuI TTCGAA 1 cut(s) 661
SinI GGWCC 4 cut(s) 746, 827, 1586, 1802
SlaI CTCGAG 1 cut(s) 502
SmiMI CAYNNNNRTG 1 cut(s) 2166
SmlI CTYRAG 2 cut(s) 502, 1847
SmoI CTYRAG 2 cut(s) 502, 1847
SphI GCATGC 1 cut(s) 218
SsiI CCGC 4 cut(s) 39, 77, 231, 263
SspI AATATT 1 cut(s) 1771
StyD4I CCNGG 7 cut(s) 103, 384, 751, 1123, 1761, 2097, 2121
StyI CCWWGG 3 cut(s) 424, 634, 1389
TaaI ACNGT 6 cut(s) 286, 448, 1624, 1637, 2001, 2188
TaiI ACGT 3 cut(s) 568, 1132, 1408
TaqI TCGA 8 cut(s) 81, 356, 503, 661, 957, 1495, 1632, 1919
TauI GCSGC 2 cut(s) 41, 79
TfiI GAWTC 3 cut(s) 202, 1052, 1183
Tru1I TTAA 9 cut(s) 591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
Tru9I TTAA 9 cut(s) 591, 717, 822, 1302, 1380, 1542, 1653, 1748, 2003
TscAI CASTG 5 cut(s) 1368, 1450, 1603, 1642, 1839
TseFI GTSAC 4 cut(s) 22, 233, 1740, 1945
TseI GCWGC 7 cut(s) 736, 1256, 1616, 1979, 2033, 2036, 2093
Tsp45I GTSAC 4 cut(s) 22, 233, 1740, 1945
TspGWI ACGGA 1 cut(s) 1933
TspRI CASTG 5 cut(s) 1368, 1450, 1603, 1642, 1839
VneI GTGCAC 1 cut(s) 1407
VpaK11BI GGWCC 4 cut(s) 746, 827, 1586, 1802
XapI RAATTY 3 cut(s) 602, 1191, 1816
XceI RCATGY 5 cut(s) 218, 698, 725, 938, 1575
XcmI CCANNNNNNNNNTGG 1 cut(s) 1528
XhoI CTCGAG 1 cut(s) 502
XmaJI CCTAGG 1 cut(s) 634
ZraI GACGTC 1 cut(s) 1130
Zsp2I ATGCAT 1 cut(s) 1735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.