Rh5CG161100

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000477
Physical Location & Seq
Forward (+)
14815826 .. 14820959
5134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG161100.1

Sequence Viewer

Length: 2301 bp
ATGGATGAGGAGGCTGGCAGGCTTAGAACTATGTACAGGGAAAAAAAATTCTCCTCGCTATTGCTTCTACGGTTTGCATTCCAAAGTCTTGGAGTGGTTTTTGGAGATTTAGGCACTTCCCCCTTGTATGTTTTCTACAATACATTTCCTCATGGAATTGCAGATCCTGAGGATGTGGTTGGAGCTCTTTCATTGATTATATACTCTCTTACTCTTATCCCACTCATCAAGTACGTATTTGTTGTCTGTCGGGCGAATGACAGCGGGCAAGGTGGAACATTTGCTCTTTATTCATTGCTCTGTCGGCATGCAAAAGTAAAAACTATTCCCAATCAGCACCGGACCGATGAAGAGCTAACAACATATAGTCGTTCTACTTTTCATGAACAATCGTATGCTGCAAAAACCAAGAAGTGGTTGGAGGGACATGCATCAAGGAAGAATGCACTTCTTCTTCTGGTCCTTGTTGGCACTTGTATGGTTATTGGAGATGGGATTCTTACACCAGCTATATCAGTTCTATCAGCTGCTGGGGGGATCAAAGTAAACAGCCCTAAGATGAATAATGATTATGTCATACTTGTAGCCGTTGTGATATTAGTGGGTTTGTTCAGCATGCAGCATTATGGGACAGATAAAGTTGGTTGGCTCTTTGCTCCCATTGTGCTGCTTTGGTTTCTCTTGATCGGAGGTATTGGCGTATTCAACATCTGGAAATATGACAGTAGTGTTTTGAGAGCTTTTTCACCTGTGTATATATATCGGTATTTTAAAAGGAATGGGAGAGATGGTTGGACTTCCCTTGGAGGTATTATGCTTAGTATAACAGGGACAGAAGCACTCTTTGCCGACCTTGCCCATTTTCCAGTTTCAGCTGTACAGATTGCTTTTACCACAGTCGTGTTTCCTTGCCTTCTTTTAGCCTATTCTGGTCAAGCTGCCTTTCTTGTAGTACATCATGATAACAATGAAACTGTGACTCAAGCATTTTATCGTTCTATTCCAGATAGGATATATTGGCCAGTCTTCGTTGTTGCGACTCTAGCTGCCATAGTTGCAAGTCAAGCAACTATATCTGCAACTTTTTCAATAATTAAGCAGGCTCTTGCACTTGGCTGTTTTCCTAGGGTCAAAGTTGTACATACATCAAAGAAGTTCCTTGGCCAAATATATATTCCAGATATTAATTGGATCCTTATGATTCTTTGCATTGCTGTGACTGCTGGATTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCGGTTGTAGTGGTCATGCTGGCAACCACTTTCCTTATGACTCTAATCATGATATTGGTGTGGCGCTGCCATTGGATTCTTGTTGTGATATTCACTGGTTTGTCACTGGTTGTGGAGTGCACTTACTTTTCTGCTGTACTCTTCAAGGTTGATCAAGGGGGATGGGTTCCTCTTGTGATTGCAGCTGCCTTTCTTCTCATCATGTATGTATGGCACTATGGTTCATTGAAACGTTATGAGTTTGAAATGCACAGCAAGGTTTCAATGGCCTGGCTTCTTGGACTTGGCCCCAGTTTAGGACTGGTTCGGGTCCCTGGGGTAGGACTTGTGTATACTGAGCTTGCAAGCGGGGTGCCCCATATCTTTTCCCACTTCATCACCAACCTGCCTGCTATCCATTCTGTTGTTATCTTTGTTTGTGTGAAGTATCTTCCTGTATATACTGTCCCAGAAGAAGAAAGATTCCTTGTAAAGCGGATTGGCCCCAAGAATTTCCACATGTTCCGGTGTGTTGCAAGGTATGGTTACAAAGACCTCCACAGAAAAGATGATGACTTTGAGAAAAAGCTGTTTGATAATCTTTTCATGTTTGTCCGACTGGAAGCCATGATGGAAGGGTGTTCAGACTCAGATGAGTACAGTGTATATGGCCAGCAAACCGAGAAGTCAAGGGAAATCCTATTAAACAATAATGGAAACACTTCTCCGTCGTTGGGGGACCTTACCATGTCAACAGTGGATGCCATTGTGCCTGTTAGATCTCCGGTGAATAATGCAAGCAATACTGTCTCTTCTCAGATGAGCAACCAGACTGAGATGGATGAGCTAGAATTTATGAATAATTGTAGAGACGCCGGCGTGGTGCATATACTTGGTAATACGGTAGTAAGAGCAAGGAGGGATTCCAGGTTCTACAAGAAGATTGCTGTTGATTATATATATGCATTTCTTAGAAAAGTATGCAGGGAGCACAGTGTGATCTTCAATGTCCCTCATGAGAGTCTCTTGAATGTTGGACAAATTTTCTACGTGTAA

Protein Analysis

766

Amino Acids

85.77

Weight (kDa)

8.8

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 26 - 513 6.2e-163 K+ potassium transporter integral membrane domain
K_trans_C PF22776 527 - 763 1.4e-25 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1659
AccB1I GGYRCC 1 cut(s) 1618
AccB7I CCANNNNNTGG 1 cut(s) 414
AccI GTMKAC 1 cut(s) 1598
AciI CCGC 4 cut(s) 264, 1268, 1614, 1741
AclI AACGTT 1 cut(s) 1498
AclWI GGATC 4 cut(s) 158, 545, 1186, 1199
AcoI YGGCCR 3 cut(s) 1019, 1162, 1915
AcsI RAATTY 4 cut(s) 47, 1756, 2096, 2286
AcyI GRCGYC 1 cut(s) 2118
AdeI CACNNNGTG 1 cut(s) 2242
AfaI GTAC 7 cut(s) 35, 233, 879, 954, 1140, 1404, 1904
AfiI CCNNNNNNNGG 4 cut(s) 414, 1562, 1586, 1979
AflIII ACRYGT 2 cut(s) 1764, 2295
AgsI TTSAA 9 cut(s) 706, 1089, 1231, 1411, 1495, 1511, 1530, 2251, 2275
AjnI CCWGG 3 cut(s) 1535, 1579, 2171
AjuI GAANNNNNNNTTGG 2 cut(s) 1158, 1190
AleI CACNNNNGTG 1 cut(s) 899
AloI GAACNNNNNNTCC 2 cut(s) 2159, 2191
Alw21I GWGCWC 3 cut(s) 187, 1388, 2238
Alw26I GTCTC 3 cut(s) 2059, 2109, 2273
Alw44I GTGCAC 1 cut(s) 1384
AlwI GGATC 4 cut(s) 158, 545, 1186, 1199
AlwNI CAGNNNCTG 2 cut(s) 167, 530
AoxI GGCC 6 cut(s) 1019, 1162, 1533, 1552, 1747, 1915
ApaLI GTGCAC 1 cut(s) 1384
ApeKI GCWGC 9 cut(s) 398, 527, 619, 667, 938, 1046, 1332, 1448, 1451
ApoI RAATTY 4 cut(s) 47, 1756, 2096, 2286
AseI ATTAAT 1 cut(s) 1185
Asp700I GAANNNNTTC 1 cut(s) 1966
AspA2I CCTAGG 1 cut(s) 1124
AspLEI GCGC 1 cut(s) 1332
AspS9I GGNCC 6 cut(s) 342, 460, 1553, 1576, 1748, 1984
AsuHPI GGTGA 3 cut(s) 738, 1636, 2044
AvaII GGWCC 4 cut(s) 342, 460, 1576, 1984
AvrII CCTAGG 1 cut(s) 1124
AxyI CCTNAGG 1 cut(s) 168
BaeGI GKGCMC 2 cut(s) 1388, 1623
BalI TGGCCA 3 cut(s) 1021, 1164, 1917
BamHI GGATCC 1 cut(s) 1191
BanI GGYRCC 1 cut(s) 1618
BanII GRGCYC 1 cut(s) 187
BbsI GAAGAC 1 cut(s) 1018
Bbv12I GWGCWC 3 cut(s) 187, 1388, 2238
BbvI GCAGC 9 cut(s) 385, 514, 631, 654, 925, 1033, 1319, 1438, 1460
BccI CCATC 5 cut(s) 485, 782, 1422, 1870, 2077
BceAI ACGGC 2 cut(s) 572, 1281
BciT130I CCWGG 3 cut(s) 1537, 1581, 2173
BclI TGATCA 1 cut(s) 1417
BcoDI GTCTC 3 cut(s) 2059, 2109, 2273
BfaI CTAG 3 cut(s) 1043, 1125, 2093
BfoI RGCGCY 1 cut(s) 1333
BfuAI ACCTGC 1 cut(s) 1659
BglII AGATCT 1 cut(s) 2024
BisI GCNGC 9 cut(s) 399, 528, 620, 668, 939, 1047, 1333, 1449, 1452
BlnI CCTAGG 1 cut(s) 1124
BlsI GCNGC 9 cut(s) 400, 529, 621, 669, 940, 1048, 1334, 1450, 1453
Bme1390I CCNGG 3 cut(s) 1537, 1581, 2173
Bme18I GGWCC 4 cut(s) 342, 460, 1576, 1984
BmgT120I GGNCC 6 cut(s) 342, 460, 1553, 1576, 1748, 1984
BmiI GGNNCC 8 cut(s) 1193, 1434, 1555, 1577, 1578, 1620, 1750, 1985
BmrFI CCNGG 3 cut(s) 1537, 1581, 2173
BmrI ACTGGG 1 cut(s) 1551
BmsI GCATC 2 cut(s) 440, 1996
BmuI ACTGGG 1 cut(s) 1551
BpiI GAAGAC 1 cut(s) 1018
BpuEI CTTGAG 1 cut(s) 966
BsaAI YACGTR 2 cut(s) 235, 2296
BsaHI GRCGYC 1 cut(s) 2118
BsaJI CCNNGG 5 cut(s) 802, 1124, 1159, 1579, 1580
BsaWI WCCGGW 3 cut(s) 339, 1770, 2029
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 4 cut(s) 414, 1562, 1586, 1979
Bse118I RCCGGY 1 cut(s) 2120
Bse1I ACTGG 7 cut(s) 866, 1022, 1366, 1377, 1557, 1572, 1869
Bse21I CCTNAGG 1 cut(s) 168
Bse3DI GCAATG 2 cut(s) 293, 1209
BseBI CCWGG 3 cut(s) 1537, 1581, 2173
BseDI CCNNGG 5 cut(s) 802, 1124, 1159, 1579, 1580
BseGI GGATG 5 cut(s) 10, 178, 1433, 2011, 2092
BseLI CCNNNNNNNGG 4 cut(s) 414, 1562, 1586, 1979
BseMI GCAATG 2 cut(s) 293, 1209
BseMII CTCAG 5 cut(s) 159, 1593, 1908, 2070, 2075
BseNI ACTGG 7 cut(s) 866, 1022, 1366, 1377, 1557, 1572, 1869
BseRI GAGGAG 2 cut(s) 23, 43
BseSI GKGCMC 2 cut(s) 1388, 1623
BseXI GCAGC 9 cut(s) 385, 514, 631, 654, 925, 1033, 1319, 1438, 1460
BseYI CCCAGC 1 cut(s) 530
BshFI GGCC 6 cut(s) 1021, 1164, 1535, 1554, 1749, 1917
BshNI GGYRCC 1 cut(s) 1618
BsiHKAI GWGCWC 3 cut(s) 187, 1388, 2238
BsiSI CCGG 4 cut(s) 340, 1771, 2030, 2121
BslFI GGGAC 8 cut(s) 438, 643, 844, 1276, 1562, 1697, 1997, 2240
BslI CCNNNNNNNGG 4 cut(s) 414, 1562, 1586, 1979
BsmAI GTCTC 3 cut(s) 2059, 2109, 2273
BsmBI CGTCTC 1 cut(s) 2109
BsmFI GGGAC 8 cut(s) 438, 643, 844, 1276, 1562, 1697, 1997, 2240
BsmI GAATGC 2 cut(s) 77, 448
BsnI GGCC 6 cut(s) 1021, 1164, 1535, 1554, 1749, 1917
Bsp1286I GDGCHC 4 cut(s) 187, 1388, 1623, 2238
Bsp1407I TGTACA 3 cut(s) 33, 877, 1138
Bsp143I GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
BspACI CCGC 4 cut(s) 264, 1268, 1614, 1741
BspANI GGCC 6 cut(s) 1021, 1164, 1535, 1554, 1749, 1917
BspCNI CTCAG 5 cut(s) 160, 1594, 1907, 2071, 2074
BspHI TCATGA 4 cut(s) 382, 958, 1314, 2260
BspLI GGNNCC 8 cut(s) 1193, 1434, 1555, 1577, 1578, 1620, 1750, 1985
BspMI ACCTGC 1 cut(s) 1659
BspPI GGATC 4 cut(s) 158, 545, 1186, 1199
BspQI GCTCTTC 1 cut(s) 345
BspT107I GGYRCC 1 cut(s) 1618
BsrDI GCAATG 2 cut(s) 293, 1209
BsrFI RCCGGY 1 cut(s) 2120
BsrGI TGTACA 3 cut(s) 33, 877, 1138
BsrI ACTGG 7 cut(s) 866, 1022, 1366, 1377, 1557, 1572, 1869
BssAI RCCGGY 1 cut(s) 2120
BssECI CCNNGG 5 cut(s) 802, 1124, 1159, 1579, 1580
BssMI GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
BssNAI GTATAC 1 cut(s) 1599
BssNI GRCGYC 1 cut(s) 2118
BssT1I CCWWGG 3 cut(s) 802, 1124, 1159
Bst1107I GTATAC 1 cut(s) 1599
Bst2UI CCWGG 3 cut(s) 1537, 1581, 2173
Bst6I CTCTTC 3 cut(s) 345, 1412, 2062
BstACI GRCGYC 1 cut(s) 2118
BstAPI GCANNNNNTGC 1 cut(s) 845
BstAUI TGTACA 3 cut(s) 33, 877, 1138
BstBAI YACGTR 2 cut(s) 235, 2296
BstDEI CTNAG 9 cut(s) 23, 168, 555, 818, 1602, 1894, 2061, 2079, 2216
BstENI CCTNNNNNAGG 1 cut(s) 1584
BstF5I GGATG 5 cut(s) 10, 178, 1433, 2011, 2092
BstH2I RGCGCY 1 cut(s) 1333
BstHHI GCGC 1 cut(s) 1332
BstKTI GATC 7 cut(s) 166, 540, 687, 1194, 1420, 2027, 2247
BstMAI GTCTC 3 cut(s) 2059, 2109, 2273
BstMBI GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
BstMWI GCNNNNNNNGC 7 cut(s) 304, 845, 854, 1043, 1055, 1064, 1220
BstNI CCWGG 3 cut(s) 1537, 1581, 2173
BstNSI RCATGY 4 cut(s) 311, 431, 619, 1768
BstSCI CCNGG 3 cut(s) 1535, 1579, 2171
BstSLI GKGCMC 2 cut(s) 1388, 1623
BstSNI TACGTA 1 cut(s) 235
BstV1I GCAGC 9 cut(s) 385, 514, 631, 654, 925, 1033, 1319, 1438, 1460
BstV2I GAAGAC 1 cut(s) 1018
BstX2I RGATCY 3 cut(s) 163, 1191, 2024
BstXI CCANNNNNNTGG 1 cut(s) 89
BstYI RGATCY 3 cut(s) 163, 1191, 2024
BstZ17I GTATAC 1 cut(s) 1599
Bsu36I CCTNAGG 1 cut(s) 168
BsuRI GGCC 6 cut(s) 1021, 1164, 1535, 1554, 1749, 1917
BtsCI GGATG 5 cut(s) 10, 178, 1433, 2011, 2092
BtsIMutI CAGTG 5 cut(s) 1359, 1370, 1912, 2007, 2245
BveI ACCTGC 1 cut(s) 1659
CaiI CAGNNNCTG 2 cut(s) 167, 530
CciI TCATGA 4 cut(s) 382, 958, 1314, 2260
CfoI GCGC 1 cut(s) 1332
Cfr10I RCCGGY 1 cut(s) 2120
Cfr13I GGNCC 6 cut(s) 342, 460, 1553, 1576, 1748, 1984
CpoI CGGWCCG 1 cut(s) 342
CseI GACGC 1 cut(s) 2126
Csp6I GTAC 7 cut(s) 34, 232, 878, 953, 1139, 1403, 1903
CspI CGGWCCG 1 cut(s) 342
CviQI GTAC 7 cut(s) 34, 232, 878, 953, 1139, 1403, 1903
DdeI CTNAG 9 cut(s) 23, 168, 555, 818, 1602, 1894, 2061, 2079, 2216
DpnI GATC 7 cut(s) 165, 539, 686, 1193, 1419, 2026, 2246
DpnII GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
DraI TTTAAA 1 cut(s) 772
DraIII CACNNNGTG 1 cut(s) 2242
EaeI YGGCCR 3 cut(s) 1019, 1162, 1915
Eam1104I CTCTTC 3 cut(s) 345, 1412, 2062
EarI CTCTTC 3 cut(s) 345, 1412, 2062
Ecl136II GAGCTC 1 cut(s) 185
Eco105I TACGTA 1 cut(s) 235
Eco130I CCWWGG 3 cut(s) 802, 1124, 1159
Eco24I GRGCYC 1 cut(s) 187
Eco47I GGWCC 4 cut(s) 342, 460, 1576, 1984
Eco53kI GAGCTC 1 cut(s) 185
Eco81I CCTNAGG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 185
EcoNI CCTNNNNNAGG 1 cut(s) 1584
EcoO109I RGGNCCY 2 cut(s) 1576, 1984
EcoRII CCWGG 3 cut(s) 1535, 1579, 2171
EcoT14I CCWWGG 3 cut(s) 802, 1124, 1159
EcoT22I ATGCAT 2 cut(s) 433, 2212
EcoT38I GRGCYC 1 cut(s) 187
ErhI CCWWGG 3 cut(s) 802, 1124, 1159
Esp3I CGTCTC 1 cut(s) 2109
FaqI GGGAC 8 cut(s) 438, 643, 844, 1276, 1562, 1697, 1997, 2240
FauI CCCGC 2 cut(s) 257, 1607
FbaI TGATCA 1 cut(s) 1417
FblI GTMKAC 1 cut(s) 1598
Fnu4HI GCNGC 9 cut(s) 399, 528, 620, 668, 939, 1047, 1333, 1449, 1452
FokI GGATG 5 cut(s) 17, 185, 1440, 2018, 2099
FriOI GRGCYC 1 cut(s) 187
Fsp4HI GCNGC 9 cut(s) 399, 528, 620, 668, 939, 1047, 1333, 1449, 1452
FspBI CTAG 3 cut(s) 1043, 1125, 2093
GlaI GCGC 1 cut(s) 1331
GluI GCNGC 9 cut(s) 399, 528, 620, 668, 939, 1047, 1333, 1449, 1452
GsaI CCCAGC 1 cut(s) 534
HaeII RGCGCY 1 cut(s) 1333
HaeIII GGCC 6 cut(s) 1021, 1164, 1535, 1554, 1749, 1917
HapII CCGG 4 cut(s) 340, 1771, 2030, 2121
HgaI GACGC 1 cut(s) 2126
HhaI GCGC 1 cut(s) 1332
Hin1I GRCGYC 1 cut(s) 2118
Hin6I GCGC 1 cut(s) 1330
HinP1I GCGC 1 cut(s) 1330
HincII GTYRAC 1 cut(s) 1998
HindII GTYRAC 1 cut(s) 1998
HpaII CCGG 4 cut(s) 340, 1771, 2030, 2121
HphI GGTGA 3 cut(s) 738, 1636, 2044
Hpy166II GTNNAC 4 cut(s) 547, 1386, 1599, 1998
Hpy188I TCNGA 5 cut(s) 689, 1862, 1891, 1897, 2064
Hpy8I GTNNAC 4 cut(s) 547, 1386, 1599, 1998
Hpy99I CGWCG 1 cut(s) 1978
HpyAV CCTTC 2 cut(s) 923, 1874
HpyCH4IV ACGT 3 cut(s) 234, 1498, 2295
HpyF10VI GCNNNNNNNGC 7 cut(s) 304, 845, 854, 1043, 1055, 1064, 1220
HpyF3I CTNAG 9 cut(s) 23, 168, 555, 818, 1602, 1894, 2061, 2079, 2216
HpySE526I ACGT 3 cut(s) 234, 1498, 2295
Hsp92I GRCGYC 1 cut(s) 2118
HspAI GCGC 1 cut(s) 1330
KflI GGGWCCC 1 cut(s) 1576
KroI GCCGGC 1 cut(s) 2120
KroNI GCCGGC 1 cut(s) 2122
Ksp22I TGATCA 1 cut(s) 1417
Kzo9I GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 3 cut(s) 182, 661, 2233
Lsp1109I GCAGC 9 cut(s) 385, 514, 631, 654, 925, 1033, 1319, 1438, 1460
LweI GCATC 2 cut(s) 440, 1996
MaeI CTAG 3 cut(s) 1043, 1125, 2093
MaeII ACGT 3 cut(s) 234, 1498, 2295
MaeIII GTNAC 4 cut(s) 976, 1216, 1368, 1790
MalI GATC 7 cut(s) 165, 539, 686, 1193, 1419, 2026, 2246
MboI GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
MflI RGATCY 3 cut(s) 163, 1191, 2024
MhlI GDGCHC 4 cut(s) 187, 1388, 1623, 2238
MlsI TGGCCA 3 cut(s) 1021, 1164, 1917
MluCI AATT 9 cut(s) 47, 156, 1092, 1186, 1245, 1756, 2096, 2107, 2286
MluNI TGGCCA 3 cut(s) 1021, 1164, 1917
MlyI GAGTC 5 cut(s) 973, 1033, 1300, 1886, 2275
MmeI TCCRAC 5 cut(s) 160, 399, 773, 1885, 2260
Mox20I TGGCCA 3 cut(s) 1021, 1164, 1917
Mph1103I ATGCAT 2 cut(s) 433, 2212
MreI CGCCGGCG 1 cut(s) 2120
MroNI GCCGGC 1 cut(s) 2120
MroXI GAANNNNTTC 1 cut(s) 1966
MscI TGGCCA 3 cut(s) 1021, 1164, 1917
MseI TTAA 4 cut(s) 771, 1095, 1185, 1949
MslI CAYNNNNRTG 3 cut(s) 476, 899, 1214
Msp20I TGGCCA 3 cut(s) 1021, 1164, 1917
MspA1I CMGCKG 4 cut(s) 264, 527, 875, 1451
MspI CCGG 4 cut(s) 340, 1771, 2030, 2121
MspR9I CCNGG 3 cut(s) 1537, 1581, 2173
Mva1269I GAATGC 2 cut(s) 77, 448
MvaI CCWGG 3 cut(s) 1537, 1581, 2173
MwoI GCNNNNNNNGC 7 cut(s) 304, 845, 854, 1043, 1055, 1064, 1220
NaeI GCCGGC 1 cut(s) 2122
NdeII GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
NgoMIV GCCGGC 1 cut(s) 2120
NlaIV GGNNCC 8 cut(s) 1193, 1434, 1555, 1577, 1578, 1620, 1750, 1985
NmuCI GTSAC 3 cut(s) 976, 1216, 1368
NsiI ATGCAT 2 cut(s) 433, 2212
NspI RCATGY 4 cut(s) 311, 431, 619, 1768
OliI CACNNNNGTG 1 cut(s) 899
PaeI GCATGC 2 cut(s) 311, 619
PagI TCATGA 4 cut(s) 382, 958, 1314, 2260
PasI CCCWGGG 1 cut(s) 1580
PciI ACATGT 1 cut(s) 1764
PciSI GCTCTTC 1 cut(s) 345
PctI GAATGC 2 cut(s) 77, 448
PdiI GCCGGC 1 cut(s) 2122
PdmI GAANNNNTTC 1 cut(s) 1966
PfeI GAWTC 6 cut(s) 496, 1201, 1227, 1342, 1728, 2168
PflMI CCANNNNNTGG 1 cut(s) 414
PkrI GCNGC 9 cut(s) 400, 529, 621, 669, 940, 1048, 1334, 1450, 1453
PleI GAGTC 5 cut(s) 973, 1033, 1300, 1886, 2274
PpsI GAGTC 5 cut(s) 973, 1033, 1300, 1886, 2274
Ppu21I YACGTR 2 cut(s) 235, 2296
PpuMI RGGWCCY 2 cut(s) 1576, 1984
PscI ACATGT 1 cut(s) 1764
PshBI ATTAAT 1 cut(s) 1185
Psp124BI GAGCTC 1 cut(s) 187
Psp1406I AACGTT 1 cut(s) 1498
Psp5II RGGWCCY 2 cut(s) 1576, 1984
Psp6I CCWGG 3 cut(s) 1535, 1579, 2171
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 3 cut(s) 1535, 1579, 2171
PspN4I GGNNCC 8 cut(s) 1193, 1434, 1555, 1577, 1578, 1620, 1750, 1985
PspPI GGNCC 6 cut(s) 342, 460, 1553, 1576, 1748, 1984
PspPPI RGGWCCY 2 cut(s) 1576, 1984
PstNI CAGNNNCTG 2 cut(s) 167, 530
PsuI RGATCY 3 cut(s) 163, 1191, 2024
PvuII CAGCTG 3 cut(s) 527, 875, 1451
RsaI GTAC 7 cut(s) 35, 233, 879, 954, 1140, 1404, 1904
RsaNI GTAC 7 cut(s) 34, 232, 878, 953, 1139, 1403, 1903
RseI CAYNNNNRTG 3 cut(s) 476, 899, 1214
Rsr2I CGGWCCG 1 cut(s) 342
RsrII CGGWCCG 1 cut(s) 342
SacI GAGCTC 1 cut(s) 187
SapI GCTCTTC 1 cut(s) 345
SaqAI TTAA 4 cut(s) 771, 1095, 1185, 1949
SatI GCNGC 9 cut(s) 399, 528, 620, 668, 939, 1047, 1333, 1449, 1452
Sau3AI GATC 7 cut(s) 163, 537, 684, 1191, 1417, 2024, 2244
Sau96I GGNCC 6 cut(s) 342, 460, 1553, 1576, 1748, 1984
SchI GAGTC 5 cut(s) 973, 1033, 1300, 1886, 2275
ScrFI CCNGG 3 cut(s) 1537, 1581, 2173
SduI GDGCHC 4 cut(s) 187, 1388, 1623, 2238
SfaNI GCATC 2 cut(s) 440, 1996
SgrAI CRCCGGYG 1 cut(s) 2120
SinI GGWCC 4 cut(s) 342, 460, 1576, 1984
SmiMI CAYNNNNRTG 3 cut(s) 476, 899, 1214
SmlI CTYRAG 1 cut(s) 981
SmoI CTYRAG 1 cut(s) 981
SnaBI TACGTA 1 cut(s) 235
SphI GCATGC 2 cut(s) 311, 619
Sse9I AATT 9 cut(s) 47, 156, 1092, 1186, 1245, 1756, 2096, 2107, 2286
SsiI CCGC 4 cut(s) 264, 1268, 1614, 1741
SspMI CTAG 3 cut(s) 1043, 1125, 2093
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 1535, 1579, 2171
StyI CCWWGG 3 cut(s) 802, 1124, 1159
TaiI ACGT 3 cut(s) 237, 1501, 2298
TaqII GACCGA 1 cut(s) 359
TasI AATT 9 cut(s) 47, 156, 1092, 1186, 1245, 1756, 2096, 2107, 2286
TatI WGTACW 6 cut(s) 33, 877, 952, 1138, 1402, 1902
TfiI GAWTC 6 cut(s) 496, 1201, 1227, 1342, 1728, 2168
Tru1I TTAA 4 cut(s) 771, 1095, 1185, 1949
Tru9I TTAA 4 cut(s) 771, 1095, 1185, 1949
TscAI CASTG 5 cut(s) 1366, 1377, 1912, 2007, 2245
TseFI GTSAC 3 cut(s) 976, 1216, 1368
TseI GCWGC 9 cut(s) 398, 527, 619, 667, 938, 1046, 1332, 1448, 1451
Tsp45I GTSAC 3 cut(s) 976, 1216, 1368
TspGWI ACGGA 1 cut(s) 1962
TspRI CASTG 5 cut(s) 1366, 1377, 1912, 2007, 2245
Van91I CCANNNNNTGG 1 cut(s) 414
VneI GTGCAC 1 cut(s) 1384
VpaK11BI GGWCC 4 cut(s) 342, 460, 1576, 1984
VspI ATTAAT 1 cut(s) 1185
XagI CCTNNNNNAGG 1 cut(s) 1584
XapI RAATTY 4 cut(s) 47, 1756, 2096, 2286
XceI RCATGY 4 cut(s) 311, 431, 619, 1768
XcmI CCANNNNNNNNNTGG 4 cut(s) 415, 1185, 1564, 1999
XmaJI CCTAGG 1 cut(s) 1124
XmiI GTMKAC 1 cut(s) 1598
XmnI GAANNNNTTC 1 cut(s) 1966
XspI CTAG 3 cut(s) 1043, 1125, 2093
Zsp2I ATGCAT 2 cut(s) 433, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.