Rh5AG150600

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000477
Physical Location & Seq
Forward (+)
15742702 .. 15747965
5264 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG150600.1

Sequence Viewer

Length: 2385 bp
ATGGATTTAGGAGGGGAGATTGGTGAAGACAGTGATACCAACAAAGGCAGTATGTGGGTTTTGGATCAGAAGCTTGACCAACCCATGGATGAGGAGGCTGGCAGGCTTAGAACTATGTACAGGGAAAAAAAATTCTCCTCGCTATTGCTTCTTCGGTTTGCATTCCAAAGTCTTGGAGTGGTTTTTGGAGATTTAGGCACTTCCCCCTTGTATGTTTTCTACAATACATTTCCTCATGGAATTGCAGATCCTGAGGATGTGGTTGGAGCTCTTTCATTGATTATATACTCTCTTACTCTTATCCCACTCATCAAGTACGTATTTGTTGTCTGTCGGGCGAATGACAGCGGGCAAGGTGGAACTTTTGCTCTTTATTCATTGCTCTGTCGGCATGCAAAAGTAAAAACTATTCCCAATCAGCATCGGACTGATGAAGAGCTAACAACATATAGTCGTTCTACTTTTCATGAACAATCATATGCTGCAAAAACCAAGAAGTGGTTGGAGGGACATGCATCAAGGAAGAATGCACTTCTTCTTCTGGTCCTTGTTGGCACTTGTATGGTTATTGGAGATGGGATTCTTACACCAGCTATATCAGTTCTATCAGCTGCTGGGGGGATCAAAGTAAACAGCCCTAAGATGGATAATGATTATGTCATACTTGTAGCCGTTGTGATATTAGTGGGTTTGTTCAGCATGCAGCATTATGGGACAGATAAAGTTGGTTGGCTCTTTGCTCCCATTGTGCTGCTTTGGTTTCTCTTGATCGGAGGTATTGGCATATTCAACATCTGGAAATATGACAGTAGTGTTTTGAGAGCTTTTTCACCTGTGTATATATATCGGTATTTTAAAAGGAATGGGAGAGATGGTTGGACTTCCCTTGGAGGTATTATGCTTAGTATAACAGGGACAGAAGCACTCTTTGCCGACCTTGCCCATTTTCCAGTTTCAGCTGTACAGATTGCTTTTACCACAGTTGTGTTTCCTTGCCTTCTTTTAGCCTATTCTGGTCAAGCTGCCTTTCTTGTAGTACATCATGATAACAATGAAACTGTGACTCAAGCATTTTATCGTTCTATTCCAGATAGGATATATTGGCCAGTCTTCGTTGTTGCGACTCTAGCTGCCATAGTTGCAAGTCAAGCAACTATATCTGCAACTTTTTCAATAATTAAGCAGGCTCTTGCACTTGGCTGTTTTCCTAGGGTCAAAGTTGTACATACATCAAAGAAGTTCCTTGGCCAAATATATATTCCAGATATTAATTGGATCCTTATGATTCTTTGCATTGCTGTGACTGCTGGATTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCGGTTGTAGTGGTCATGCTGGCAACCACTTTCCTTATGACTCTAATCATGATATTGGTGTGGCGCTGCCATTGGATTCTTGTTGTGATATTCACTGGTTTGTCACTGGTTGTGGAGTGCACTTACTTTTCTGCTGTACTCTTCAAGGTTGATCAAGGGGGATGGGTTCCTCTTGTGATTGCAGCTGCCTTTCTTCTCATCATGTATGTATGGCACTATGGTTCGTTGAAACGTTATGAGTTTGAAATGCACAGCAAGGTTTCAATGGCCTGGCTTCTTGGACTTGGCCCCAGTTTAGGACTGGTTCGGGTCCCTGGGGTAGGACTTGTGTATACCGAGCTTGCAAGCGGGGTGCCCCATATCTTTTCCCACTTCATTACCAACCTGCCTGCTATCCATTCTGTTGTTATCTTTGTTTGTGTGAAGTATCTTCCTGTATATACTGTCCCAGAAGAAGAAAGATTCCTTGTAAAGCGGATTGGCCCCAAGAATTTCCACATGTTCCGGTGTGTTGCTAGATATGGTTACAAAGACCTCCACAGAAAAGATGATGACTTTGAGAAAAAGCTGTTTGATAATCTTTTCATGTTTGTCCGATTGGAAGCCATGATGGAAGGGTGTTCAGACTCAGATGAGTACAGTGTATATGGCCAGCAAACCGAGAAGTCAAGGGAAATCCTATTAAACAATAATGGAAACACTTCTCCGTCGTTGGGGGACCTTACCATGTCAACAGTGGATGCCATTGTGCCTGTTAGATCTCCGATGAATAATGCAAGCAACACTGTCTCTTCTCAGATGAGCAACCAGACTGAGATGGATGAGTTGGAATTTATGAATAATTGTAGAGACGCCGGCGTGGTGCATATACTTGGTAATACGGTAGTAAGAGCAAGGAGGGATTCCAGGTTCTACAAGAAGATTGCTGTTGATTATATATATGCATTTCTTAGAAAAGTATGCAGGGAGCACAGTGTGATCTTCAATGTCCCTCATGAGAGTCTCTTGAATGTTGGACAAATTTTCTACGTGTAA

Protein Analysis

794

Amino Acids

88.87

Weight (kDa)

8.25

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 54 - 541 5.1e-163 K+ potassium transporter integral membrane domain
K_trans_C PF22776 555 - 791 1.5e-25 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1743
AccB1I GGYRCC 1 cut(s) 1702
AccB7I CCANNNNNTGG 2 cut(s) 85, 498
AccI GTMKAC 1 cut(s) 1682
AciI CCGC 4 cut(s) 348, 1352, 1698, 1825
AclI AACGTT 1 cut(s) 1582
AclWI GGATC 5 cut(s) 72, 242, 629, 1270, 1283
AcoI YGGCCR 3 cut(s) 1103, 1246, 1999
AcsI RAATTY 4 cut(s) 131, 1840, 2180, 2370
AcyI GRCGYC 1 cut(s) 2202
AdeI CACNNNGTG 1 cut(s) 2326
AfaI GTAC 7 cut(s) 119, 317, 963, 1038, 1224, 1488, 1988
AfiI CCNNNNNNNGG 6 cut(s) 85, 498, 643, 1646, 1670, 2063
AflIII ACRYGT 2 cut(s) 1848, 2379
AgsI TTSAA 9 cut(s) 790, 1173, 1315, 1495, 1579, 1595, 1614, 2335, 2359
AjnI CCWGG 3 cut(s) 1619, 1663, 2255
AjuI GAANNNNNNNTTGG 2 cut(s) 1242, 1274
AleI CACNNNNGTG 1 cut(s) 983
AloI GAACNNNNNNTCC 2 cut(s) 2243, 2275
Alw21I GWGCWC 3 cut(s) 271, 1472, 2322
Alw26I GTCTC 3 cut(s) 2143, 2193, 2357
Alw44I GTGCAC 1 cut(s) 1468
AlwI GGATC 5 cut(s) 72, 242, 629, 1270, 1283
AlwNI CAGNNNCTG 2 cut(s) 251, 614
AoxI GGCC 6 cut(s) 1103, 1246, 1617, 1636, 1831, 1999
ApaLI GTGCAC 1 cut(s) 1468
ApeKI GCWGC 9 cut(s) 482, 611, 703, 751, 1022, 1130, 1416, 1532, 1535
ApoI RAATTY 4 cut(s) 131, 1840, 2180, 2370
AseI ATTAAT 1 cut(s) 1269
Asp700I GAANNNNTTC 1 cut(s) 2050
AspA2I CCTAGG 1 cut(s) 1208
AspLEI GCGC 1 cut(s) 1416
AspS9I GGNCC 5 cut(s) 544, 1637, 1660, 1832, 2068
AsuHPI GGTGA 2 cut(s) 35, 822
AvaII GGWCC 3 cut(s) 544, 1660, 2068
AvrII CCTAGG 1 cut(s) 1208
AxyI CCTNAGG 1 cut(s) 252
BaeGI GKGCMC 2 cut(s) 1472, 1707
BalI TGGCCA 3 cut(s) 1105, 1248, 2001
BamHI GGATCC 1 cut(s) 1275
BanI GGYRCC 1 cut(s) 1702
BanII GRGCYC 1 cut(s) 271
BbsI GAAGAC 2 cut(s) 33, 1102
Bbv12I GWGCWC 3 cut(s) 271, 1472, 2322
BbvI GCAGC 9 cut(s) 469, 598, 715, 738, 1009, 1117, 1403, 1522, 1544
BccI CCATC 6 cut(s) 569, 637, 866, 1506, 1954, 2161
BceAI ACGGC 2 cut(s) 656, 1365
BciT130I CCWGG 3 cut(s) 1621, 1665, 2257
BclI TGATCA 1 cut(s) 1501
BcoDI GTCTC 3 cut(s) 2143, 2193, 2357
BfaI CTAG 3 cut(s) 1127, 1209, 1866
BfoI RGCGCY 1 cut(s) 1417
BfuAI ACCTGC 1 cut(s) 1743
BglII AGATCT 1 cut(s) 2108
BisI GCNGC 9 cut(s) 483, 612, 704, 752, 1023, 1131, 1417, 1533, 1536
BlnI CCTAGG 1 cut(s) 1208
BlsI GCNGC 9 cut(s) 484, 613, 705, 753, 1024, 1132, 1418, 1534, 1537
Bme1390I CCNGG 3 cut(s) 1621, 1665, 2257
Bme18I GGWCC 3 cut(s) 544, 1660, 2068
BmgT120I GGNCC 5 cut(s) 544, 1637, 1660, 1832, 2068
BmiI GGNNCC 8 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834, 2069
BmrFI CCNGG 3 cut(s) 1621, 1665, 2257
BmrI ACTGGG 1 cut(s) 1635
BmsI GCATC 3 cut(s) 430, 524, 2080
BmuI ACTGGG 1 cut(s) 1635
BpiI GAAGAC 2 cut(s) 33, 1102
BpuEI CTTGAG 1 cut(s) 1050
BsaAI YACGTR 2 cut(s) 319, 2380
BsaHI GRCGYC 1 cut(s) 2202
BsaJI CCNNGG 6 cut(s) 84, 886, 1208, 1243, 1663, 1664
BsaWI WCCGGW 1 cut(s) 1854
BsaXI ACNNNNNCTCC 2 cut(s) 859, 889
Bsc4I CCNNNNNNNGG 6 cut(s) 85, 498, 643, 1646, 1670, 2063
Bse118I RCCGGY 1 cut(s) 2204
Bse1I ACTGG 6 cut(s) 950, 1106, 1450, 1461, 1641, 1656
Bse21I CCTNAGG 1 cut(s) 252
Bse3DI GCAATG 2 cut(s) 377, 1293
BseBI CCWGG 3 cut(s) 1621, 1665, 2257
BseDI CCNNGG 6 cut(s) 84, 886, 1208, 1243, 1663, 1664
BseGI GGATG 5 cut(s) 94, 262, 1517, 2095, 2176
BseLI CCNNNNNNNGG 6 cut(s) 85, 498, 643, 1646, 1670, 2063
BseMI GCAATG 2 cut(s) 377, 1293
BseMII CTCAG 4 cut(s) 243, 1992, 2154, 2159
BseNI ACTGG 6 cut(s) 950, 1106, 1450, 1461, 1641, 1656
BseRI GAGGAG 2 cut(s) 107, 127
BseSI GKGCMC 2 cut(s) 1472, 1707
BseXI GCAGC 9 cut(s) 469, 598, 715, 738, 1009, 1117, 1403, 1522, 1544
BseYI CCCAGC 1 cut(s) 614
BshFI GGCC 6 cut(s) 1105, 1248, 1619, 1638, 1833, 2001
BshNI GGYRCC 1 cut(s) 1702
BsiHKAI GWGCWC 3 cut(s) 271, 1472, 2322
BsiSI CCGG 2 cut(s) 1855, 2205
BslFI GGGAC 8 cut(s) 522, 727, 928, 1360, 1646, 1781, 2081, 2324
BslI CCNNNNNNNGG 6 cut(s) 85, 498, 643, 1646, 1670, 2063
BsmAI GTCTC 3 cut(s) 2143, 2193, 2357
BsmBI CGTCTC 1 cut(s) 2193
BsmFI GGGAC 8 cut(s) 522, 727, 928, 1360, 1646, 1781, 2081, 2324
BsmI GAATGC 2 cut(s) 161, 532
BsnI GGCC 6 cut(s) 1105, 1248, 1619, 1638, 1833, 2001
Bsp1286I GDGCHC 4 cut(s) 271, 1472, 1707, 2322
Bsp1407I TGTACA 3 cut(s) 117, 961, 1222
Bsp143I GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 4 cut(s) 348, 1352, 1698, 1825
BspANI GGCC 6 cut(s) 1105, 1248, 1619, 1638, 1833, 2001
BspCNI CTCAG 4 cut(s) 244, 1991, 2155, 2158
BspHI TCATGA 4 cut(s) 466, 1042, 1398, 2344
BspLI GGNNCC 8 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834, 2069
BspMI ACCTGC 1 cut(s) 1743
BspPI GGATC 5 cut(s) 72, 242, 629, 1270, 1283
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 1702
BsrDI GCAATG 2 cut(s) 377, 1293
BsrFI RCCGGY 1 cut(s) 2204
BsrGI TGTACA 3 cut(s) 117, 961, 1222
BsrI ACTGG 6 cut(s) 950, 1106, 1450, 1461, 1641, 1656
BssAI RCCGGY 1 cut(s) 2204
BssECI CCNNGG 6 cut(s) 84, 886, 1208, 1243, 1663, 1664
BssMI GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
BssNAI GTATAC 1 cut(s) 1683
BssNI GRCGYC 1 cut(s) 2202
BssT1I CCWWGG 4 cut(s) 84, 886, 1208, 1243
Bst1107I GTATAC 1 cut(s) 1683
Bst2UI CCWGG 3 cut(s) 1621, 1665, 2257
Bst6I CTCTTC 3 cut(s) 429, 1496, 2146
BstACI GRCGYC 1 cut(s) 2202
BstAPI GCANNNNNTGC 1 cut(s) 929
BstAUI TGTACA 3 cut(s) 117, 961, 1222
BstBAI YACGTR 2 cut(s) 319, 2380
BstDEI CTNAG 8 cut(s) 107, 252, 639, 902, 1978, 2145, 2163, 2300
BstDSI CCRYGG 1 cut(s) 84
BstENI CCTNNNNNAGG 1 cut(s) 1668
BstF5I GGATG 5 cut(s) 94, 262, 1517, 2095, 2176
BstH2I RGCGCY 1 cut(s) 1417
BstHHI GCGC 1 cut(s) 1416
BstKTI GATC 8 cut(s) 67, 250, 624, 771, 1278, 1504, 2111, 2331
BstMAI GTCTC 3 cut(s) 2143, 2193, 2357
BstMBI GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
BstMWI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
BstNI CCWGG 3 cut(s) 1621, 1665, 2257
BstNSI RCATGY 4 cut(s) 395, 515, 703, 1852
BstSCI CCNGG 3 cut(s) 1619, 1663, 2255
BstSLI GKGCMC 2 cut(s) 1472, 1707
BstSNI TACGTA 1 cut(s) 319
BstV1I GCAGC 9 cut(s) 469, 598, 715, 738, 1009, 1117, 1403, 1522, 1544
BstV2I GAAGAC 2 cut(s) 33, 1102
BstX2I RGATCY 3 cut(s) 247, 1275, 2108
BstXI CCANNNNNNTGG 1 cut(s) 173
BstYI RGATCY 3 cut(s) 247, 1275, 2108
BstZ17I GTATAC 1 cut(s) 1683
Bsu36I CCTNAGG 1 cut(s) 252
BsuRI GGCC 6 cut(s) 1105, 1248, 1619, 1638, 1833, 2001
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 5 cut(s) 94, 262, 1517, 2095, 2176
BtsIMutI CAGTG 7 cut(s) 37, 1443, 1454, 1996, 2091, 2133, 2329
BveI ACCTGC 1 cut(s) 1743
CaiI CAGNNNCTG 2 cut(s) 251, 614
CciI TCATGA 4 cut(s) 466, 1042, 1398, 2344
CfoI GCGC 1 cut(s) 1416
Cfr10I RCCGGY 1 cut(s) 2204
Cfr13I GGNCC 5 cut(s) 544, 1637, 1660, 1832, 2068
CseI GACGC 1 cut(s) 2210
Csp6I GTAC 7 cut(s) 118, 316, 962, 1037, 1223, 1487, 1987
CviQI GTAC 7 cut(s) 118, 316, 962, 1037, 1223, 1487, 1987
DdeI CTNAG 8 cut(s) 107, 252, 639, 902, 1978, 2145, 2163, 2300
DpnI GATC 8 cut(s) 66, 249, 623, 770, 1277, 1503, 2110, 2330
DpnII GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
DraI TTTAAA 1 cut(s) 856
DraIII CACNNNGTG 1 cut(s) 2326
EaeI YGGCCR 3 cut(s) 1103, 1246, 1999
Eam1104I CTCTTC 3 cut(s) 429, 1496, 2146
EarI CTCTTC 3 cut(s) 429, 1496, 2146
Ecl136II GAGCTC 1 cut(s) 269
Eco105I TACGTA 1 cut(s) 319
Eco130I CCWWGG 4 cut(s) 84, 886, 1208, 1243
Eco24I GRGCYC 1 cut(s) 271
Eco47I GGWCC 3 cut(s) 544, 1660, 2068
Eco53kI GAGCTC 1 cut(s) 269
Eco81I CCTNAGG 1 cut(s) 252
EcoICRI GAGCTC 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 1668
EcoO109I RGGNCCY 2 cut(s) 1660, 2068
EcoRII CCWGG 3 cut(s) 1619, 1663, 2255
EcoT14I CCWWGG 4 cut(s) 84, 886, 1208, 1243
EcoT22I ATGCAT 2 cut(s) 517, 2296
EcoT38I GRGCYC 1 cut(s) 271
ErhI CCWWGG 4 cut(s) 84, 886, 1208, 1243
Esp3I CGTCTC 1 cut(s) 2193
FaqI GGGAC 8 cut(s) 522, 727, 928, 1360, 1646, 1781, 2081, 2324
FauI CCCGC 2 cut(s) 341, 1691
FauNDI CATATG 1 cut(s) 478
FbaI TGATCA 1 cut(s) 1501
FblI GTMKAC 1 cut(s) 1682
Fnu4HI GCNGC 9 cut(s) 483, 612, 704, 752, 1023, 1131, 1417, 1533, 1536
FokI GGATG 5 cut(s) 101, 269, 1524, 2102, 2183
FriOI GRGCYC 1 cut(s) 271
Fsp4HI GCNGC 9 cut(s) 483, 612, 704, 752, 1023, 1131, 1417, 1533, 1536
FspBI CTAG 3 cut(s) 1127, 1209, 1866
GlaI GCGC 1 cut(s) 1415
GluI GCNGC 9 cut(s) 483, 612, 704, 752, 1023, 1131, 1417, 1533, 1536
GsaI CCCAGC 1 cut(s) 618
HaeII RGCGCY 1 cut(s) 1417
HaeIII GGCC 6 cut(s) 1105, 1248, 1619, 1638, 1833, 2001
HapII CCGG 2 cut(s) 1855, 2205
HgaI GACGC 1 cut(s) 2210
HhaI GCGC 1 cut(s) 1416
Hin1I GRCGYC 1 cut(s) 2202
Hin6I GCGC 1 cut(s) 1414
HinP1I GCGC 1 cut(s) 1414
HincII GTYRAC 1 cut(s) 2082
HindII GTYRAC 1 cut(s) 2082
HindIII AAGCTT 1 cut(s) 71
HpaII CCGG 2 cut(s) 1855, 2205
HphI GGTGA 2 cut(s) 35, 822
Hpy166II GTNNAC 4 cut(s) 631, 1470, 1683, 2082
Hpy188I TCNGA 8 cut(s) 69, 426, 773, 1946, 1975, 1981, 2115, 2148
Hpy8I GTNNAC 4 cut(s) 631, 1470, 1683, 2082
Hpy99I CGWCG 1 cut(s) 2062
HpyAV CCTTC 2 cut(s) 1007, 1958
HpyCH4IV ACGT 3 cut(s) 318, 1582, 2379
HpyF10VI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
HpyF3I CTNAG 8 cut(s) 107, 252, 639, 902, 1978, 2145, 2163, 2300
HpySE526I ACGT 3 cut(s) 318, 1582, 2379
Hsp92I GRCGYC 1 cut(s) 2202
HspAI GCGC 1 cut(s) 1414
KflI GGGWCCC 1 cut(s) 1660
KroI GCCGGC 1 cut(s) 2204
KroNI GCCGGC 1 cut(s) 2206
Ksp22I TGATCA 1 cut(s) 1501
Kzo9I GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 3 cut(s) 266, 745, 2317
Lsp1109I GCAGC 9 cut(s) 469, 598, 715, 738, 1009, 1117, 1403, 1522, 1544
LweI GCATC 3 cut(s) 430, 524, 2080
MaeI CTAG 3 cut(s) 1127, 1209, 1866
MaeII ACGT 3 cut(s) 318, 1582, 2379
MaeIII GTNAC 4 cut(s) 1060, 1300, 1452, 1874
MalI GATC 8 cut(s) 66, 249, 623, 770, 1277, 1503, 2110, 2330
MboI GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
MflI RGATCY 3 cut(s) 247, 1275, 2108
MhlI GDGCHC 4 cut(s) 271, 1472, 1707, 2322
MlsI TGGCCA 3 cut(s) 1105, 1248, 2001
MluCI AATT 9 cut(s) 131, 240, 1176, 1270, 1329, 1840, 2180, 2191, 2370
MluNI TGGCCA 3 cut(s) 1105, 1248, 2001
MlyI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2359
MmeI TCCRAC 5 cut(s) 244, 483, 857, 2157, 2344
Mox20I TGGCCA 3 cut(s) 1105, 1248, 2001
Mph1103I ATGCAT 2 cut(s) 517, 2296
MreI CGCCGGCG 1 cut(s) 2204
MroNI GCCGGC 1 cut(s) 2204
MroXI GAANNNNTTC 1 cut(s) 2050
MscI TGGCCA 3 cut(s) 1105, 1248, 2001
MseI TTAA 4 cut(s) 855, 1179, 1269, 2033
MslI CAYNNNNRTG 3 cut(s) 560, 983, 1298
Msp20I TGGCCA 3 cut(s) 1105, 1248, 2001
MspA1I CMGCKG 4 cut(s) 348, 611, 959, 1535
MspI CCGG 2 cut(s) 1855, 2205
MspR9I CCNGG 3 cut(s) 1621, 1665, 2257
Mva1269I GAATGC 2 cut(s) 161, 532
MvaI CCWGG 3 cut(s) 1621, 1665, 2257
MwoI GCNNNNNNNGC 7 cut(s) 388, 929, 938, 1127, 1139, 1148, 1304
NaeI GCCGGC 1 cut(s) 2206
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 478
NdeII GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
NgoMIV GCCGGC 1 cut(s) 2204
NlaIV GGNNCC 8 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834, 2069
NmuCI GTSAC 3 cut(s) 1060, 1300, 1452
NsiI ATGCAT 2 cut(s) 517, 2296
NspI RCATGY 4 cut(s) 395, 515, 703, 1852
OliI CACNNNNGTG 1 cut(s) 983
PaeI GCATGC 2 cut(s) 395, 703
PagI TCATGA 4 cut(s) 466, 1042, 1398, 2344
PasI CCCWGGG 1 cut(s) 1664
PciI ACATGT 1 cut(s) 1848
PciSI GCTCTTC 1 cut(s) 429
PctI GAATGC 2 cut(s) 161, 532
PdiI GCCGGC 1 cut(s) 2206
PdmI GAANNNNTTC 1 cut(s) 2050
PfeI GAWTC 6 cut(s) 580, 1285, 1311, 1426, 1812, 2252
PflMI CCANNNNNTGG 2 cut(s) 85, 498
PkrI GCNGC 9 cut(s) 484, 613, 705, 753, 1024, 1132, 1418, 1534, 1537
PleI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2358
PpsI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2358
Ppu21I YACGTR 2 cut(s) 319, 2380
PpuMI RGGWCCY 2 cut(s) 1660, 2068
PscI ACATGT 1 cut(s) 1848
PshBI ATTAAT 1 cut(s) 1269
Psp124BI GAGCTC 1 cut(s) 271
Psp1406I AACGTT 1 cut(s) 1582
Psp5II RGGWCCY 2 cut(s) 1660, 2068
Psp6I CCWGG 3 cut(s) 1619, 1663, 2255
PspFI CCCAGC 1 cut(s) 614
PspGI CCWGG 3 cut(s) 1619, 1663, 2255
PspN4I GGNNCC 8 cut(s) 1277, 1518, 1639, 1661, 1662, 1704, 1834, 2069
PspPI GGNCC 5 cut(s) 544, 1637, 1660, 1832, 2068
PspPPI RGGWCCY 2 cut(s) 1660, 2068
PstNI CAGNNNCTG 2 cut(s) 251, 614
PsuI RGATCY 3 cut(s) 247, 1275, 2108
PvuII CAGCTG 3 cut(s) 611, 959, 1535
RsaI GTAC 7 cut(s) 119, 317, 963, 1038, 1224, 1488, 1988
RsaNI GTAC 7 cut(s) 118, 316, 962, 1037, 1223, 1487, 1987
RseI CAYNNNNRTG 3 cut(s) 560, 983, 1298
SacI GAGCTC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 855, 1179, 1269, 2033
SatI GCNGC 9 cut(s) 483, 612, 704, 752, 1023, 1131, 1417, 1533, 1536
Sau3AI GATC 8 cut(s) 64, 247, 621, 768, 1275, 1501, 2108, 2328
Sau96I GGNCC 5 cut(s) 544, 1637, 1660, 1832, 2068
SchI GAGTC 5 cut(s) 1057, 1117, 1384, 1970, 2359
ScrFI CCNGG 3 cut(s) 1621, 1665, 2257
SduI GDGCHC 4 cut(s) 271, 1472, 1707, 2322
SfaNI GCATC 3 cut(s) 430, 524, 2080
SgrAI CRCCGGYG 1 cut(s) 2204
SinI GGWCC 3 cut(s) 544, 1660, 2068
SmiMI CAYNNNNRTG 3 cut(s) 560, 983, 1298
SmlI CTYRAG 1 cut(s) 1065
SmoI CTYRAG 1 cut(s) 1065
SnaBI TACGTA 1 cut(s) 319
SphI GCATGC 2 cut(s) 395, 703
Sse9I AATT 9 cut(s) 131, 240, 1176, 1270, 1329, 1840, 2180, 2191, 2370
SsiI CCGC 4 cut(s) 348, 1352, 1698, 1825
SspMI CTAG 3 cut(s) 1127, 1209, 1866
SstI GAGCTC 1 cut(s) 271
StyD4I CCNGG 3 cut(s) 1619, 1663, 2255
StyI CCWWGG 4 cut(s) 84, 886, 1208, 1243
TaiI ACGT 3 cut(s) 321, 1585, 2382
TasI AATT 9 cut(s) 131, 240, 1176, 1270, 1329, 1840, 2180, 2191, 2370
TatI WGTACW 6 cut(s) 117, 961, 1036, 1222, 1486, 1986
TfiI GAWTC 6 cut(s) 580, 1285, 1311, 1426, 1812, 2252
Tru1I TTAA 4 cut(s) 855, 1179, 1269, 2033
Tru9I TTAA 4 cut(s) 855, 1179, 1269, 2033
TscAI CASTG 7 cut(s) 37, 1450, 1461, 1996, 2091, 2140, 2329
TseFI GTSAC 3 cut(s) 1060, 1300, 1452
TseI GCWGC 9 cut(s) 482, 611, 703, 751, 1022, 1130, 1416, 1532, 1535
Tsp45I GTSAC 3 cut(s) 1060, 1300, 1452
TspGWI ACGGA 1 cut(s) 2046
TspRI CASTG 7 cut(s) 37, 1450, 1461, 1996, 2091, 2140, 2329
Van91I CCANNNNNTGG 2 cut(s) 85, 498
VneI GTGCAC 1 cut(s) 1468
VpaK11BI GGWCC 3 cut(s) 544, 1660, 2068
VspI ATTAAT 1 cut(s) 1269
XagI CCTNNNNNAGG 1 cut(s) 1668
XapI RAATTY 4 cut(s) 131, 1840, 2180, 2370
XceI RCATGY 4 cut(s) 395, 515, 703, 1852
XcmI CCANNNNNNNNNTGG 4 cut(s) 499, 1269, 1648, 2083
XmaJI CCTAGG 1 cut(s) 1208
XmiI GTMKAC 1 cut(s) 1682
XmnI GAANNNNTTC 1 cut(s) 2050
XspI CTAG 3 cut(s) 1127, 1209, 1866
Zsp2I ATGCAT 2 cut(s) 517, 2296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.