Prupe.4G114000_v2.0.a1

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000477
Physical Location & Seq
Forward (+)
6083728 .. 6090267
6540 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G114000.1

Sequence Viewer

Length: 2505 bp
ATGCTTATTGATCATGTGGCTGAGCCTCAGCCTCTTCTCCAGTTGACCCCACTTGGGAATTCCAGTGACAAATTTTTCTATTATAGCTTTATGAAAGCTGCAGAATCATTTGAAATTTCAATGGCTTCAAGAGTGGAGATTGATGAAGATAGTGATACCAACAAAGGCAGTATGTGGGTTTTGGATCAGAAGCTTGACCAACCCATGGATGAGGAGGCTGGCAGGCTTAGAAATATGTACAGGGAAAAGAAGTTCTCTGCACTATTGCTTATGCGGCTTGCATTCCAAAGTCTTGGAGTGGTTTTTGGAGATTTGGGTACTTCTCCCTTGTATGTTTTCTACAATACGTTTCCTCATGGAATTTCAGACCCGGAGGATGTGGTTGGAGCTCTTTCATTGATTATATACTCTCTTACTCTTATCCCGCTCCTCAAGTATGTGTTCGTTGTTTGTAGGGCGAATGACAGTGGTCAAGGTGGAACATTTGCTCTTTATTCATTGCTCTGCCGACATGCAAATGTAAAAACTATTCCTAACCAACACCGGACTGATGAAGAGCTAACAACGTATAGTCGTTCTACTTTCCATGAACAATCATATGCTGCAAAAACCAAGAAGTGGTTGGAGGGGCATGCATCAAGGAAGAATGCCCTTCTTCTTCTGGTCCTCGTTGGCACTTGTATGGTGATTGGTGATGGGATTCTTACTCCAGCTATATCTGTTTTATCAGCTGCTGGCGGGATCAAAGTAAGCAGCCCTGGCATGAAAAATGATTATGTCATACTTGTTGCTGTTGTAATATTAGTAGGCTTGTTTAGCGTACAGCACTATGGTACAGATAAAGTTGGTTGGCTCTTTGCTCCCATTGTGCTTCTTTGGTTTCTCTTAATTGGAGGTATTGGCATATTCAACATCTGGAAACATGACAGTAGTGTTTTGAGAGCTTTTTCGCCCGTCTATATATATCGGTATTTTAAAAGGAATGGGAGAGATGGTTGGACTTCCCTTGGAGGTATCATGCTTAGTATAACAGGGACTGAAGCACTCTTTGCCGACCTTGCCCATTTTCCAGTTTCAGCTGTACAGATTGCTTTCACTACGGTTGTGTTTCCTTGCCTTCTTTTAGCCTATTCTGGTCAAGCTGCGTACCTTATGAAAAATCATGACAACAAAACTGTGCTTCAAGCATTTTATCTTTCCATTCCAGAGAAAATATATTGGCCAGTCTTCATTGTTGCGACTCTAGCTGCAGTAGTTGCAAGTCAAGCAACTATATCGGCGACTTTTTCAATAATTAAGCAGGCTCTTGCACTTGGCTGTTTTCCTAGAGTCAAAGTTGTACACACATCAAAGAAGTTCCTCGGCCAAATATATATTCCAGATATTAATTGGATTCTTATGATTCTTTGCATTGCTGTGACTGCTGGATTCAAAAATCAAAGCCAAATTGGAAATGCGTATGGGACGGCAGTTGTCGTGGTCATGCTAGCAACCACACTGCTCATGACTCTAATCATGATATTAGTGTGGCGCTGTCATTGGATTCTTGTCCTGATATTCACTGGTTTGTCACTGGTTGTGGAATGCACTTACTTTTCCGCTGTCCTCTTCAAGGTTGATCAAGGTGGATGGGTTCCTCTTGTGATTGCAGCTGCCTTTCTTCTCATCATGTATGTTTGGCATTATGGCACATTGAAACGTTATGAGTTTGAGATGCACAGTAAGGTTTCAATGGCATGGCTTCTTGGACTTGGCCCCAGTTTAGGACTGGTTCGGGTCCCTGGGATAGGACTTGTGTACACCGAGCTTGCAAGTGGAGTGCCTCACATCTTTTCCCACTTCATCACCAACCTGCCTGCGATCCATTCTGTTGTCGTCTTTGTTTGTGTGAAGTATCTTCCTGTATACACTGTCCCAGAAGAAGAAAGATTCCTTGTAAAGCGGATTGGCCCCAAGAGTTTCCACATGTTCCGTTGCGTTGCAAGATACGGTTACAAAGACCTCCACAAGAAAGATGATGACTTTGAGAAAAAACTCTTCGACAACCTTTTCATGTTTGTCCGGCTCGAGTCCATGATGGAGGGGTGTTCAGACTCGGATGAGTACAGTATATATGGCCAGCAAACTGAGAGGTCAATGGAAGGCCTAATAAATAATAATGGCAACACAATTGGATCGACTGCAGACCTTACTATCTCATCAGTGGACTCAATTGTGCCTGCTAAATCTCCATTGCATGCAAACAATACTATGAGTTCATCCAGTCAACAGAGCATGCAGAATGAGATTGATGAGCTAGAATTTTTGAATAATTGTAGAGATGCTGGGGTGGTGCATATACTTGGGAACACGGTTGTGAGAGCAAGGAGGGATTCCAGGTTCTACAAAAAGATAGCTGTTGATTACATTTATGCATTTCTTAGAAAAGTTTGCAGGGAGCACAGTGTGATCTTCAATGTTCCTCATGAGAGTCTTTTGAATGTTGGACAGATTTTCTATGTGTAA

Protein Analysis

835

Amino Acids

93.23

Weight (kDa)

8.07

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1860
AccB7I CCANNNNNTGG 2 cut(s) 205, 618
AccBSI CCGCTC 1 cut(s) 427
AccI GTMKAC 1 cut(s) 1905
AciI CCGC 5 cut(s) 274, 425, 738, 1599, 1942
AclI AACGTT 1 cut(s) 1699
AclWI GGATC 4 cut(s) 192, 749, 1855, 2182
AcoI YGGCCR 3 cut(s) 1220, 1363, 2116
AcsI RAATTY 5 cut(s) 58, 71, 114, 360, 2300
AcuI CTGAAG 1 cut(s) 1059
AdeI CACNNNGTG 1 cut(s) 2446
AfaI GTAC 9 cut(s) 239, 319, 822, 835, 1083, 1148, 1341, 1799, 2105
AfiI CCNNNNNNNGG 6 cut(s) 54, 205, 618, 1612, 1763, 1787
AflIII ACRYGT 1 cut(s) 1965
AjnI CCWGG 3 cut(s) 757, 1780, 2375
AjuI GAANNNNNNNTTGG 2 cut(s) 1359, 1391
AleI CACNNNNGTG 1 cut(s) 2354
AloI GAACNNNNNNTCC 4 cut(s) 236, 268, 2363, 2395
Alw21I GWGCWC 2 cut(s) 391, 2442
AlwI GGATC 4 cut(s) 192, 749, 1855, 2182
AlwNI CAGNNNCTG 2 cut(s) 734, 1037
Ama87I CYCGRG 1 cut(s) 2066
AoxI GGCC 6 cut(s) 1220, 1363, 1753, 1948, 2116, 2143
ApeKI GCWGC 8 cut(s) 98, 602, 731, 753, 1142, 1247, 1649, 1652
ApoI RAATTY 5 cut(s) 58, 71, 114, 360, 2300
AseI ATTAAT 1 cut(s) 1386
AspLEI GCGC 1 cut(s) 1533
AspS9I GGNCC 4 cut(s) 664, 1754, 1777, 1949
AsuC2I CCSGG 1 cut(s) 371
AsuHPI GGTGA 3 cut(s) 697, 704, 1837
AsuNHI GCTAGC 1 cut(s) 1486
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 2 cut(s) 664, 1777
BalI TGGCCA 2 cut(s) 1222, 2118
BanII GRGCYC 1 cut(s) 391
BbsI GAAGAC 1 cut(s) 1219
Bbv12I GWGCWC 2 cut(s) 391, 2442
BbvCI CCTCAGC 1 cut(s) 27
BbvI GCAGC 8 cut(s) 85, 589, 718, 765, 1129, 1234, 1639, 1661
BccI CCATC 4 cut(s) 689, 986, 1623, 2071
BceAI ACGGC 1 cut(s) 1482
BcgI CGANNNNNNTGC 2 cut(s) 1257, 1291
BciT130I CCWGG 3 cut(s) 759, 1782, 2377
BclI TGATCA 2 cut(s) 10, 1618
BcnI CCSGG 1 cut(s) 371
BfaI CTAG 4 cut(s) 1244, 1326, 1487, 2297
BfmI CTRYAG 3 cut(s) 99, 1248, 2181
BfoI RGCGCY 1 cut(s) 1534
BfuAI ACCTGC 1 cut(s) 1860
BisI GCNGC 9 cut(s) 99, 275, 603, 732, 754, 1143, 1248, 1650, 1653
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 9 cut(s) 100, 276, 604, 733, 755, 1144, 1249, 1651, 1654
Bme1390I CCNGG 4 cut(s) 371, 759, 1782, 2377
Bme18I GGWCC 2 cut(s) 664, 1777
BmeT110I CYCGRG 1 cut(s) 2066
BmgT120I GGNCC 4 cut(s) 664, 1754, 1777, 1949
BmiI GGNNCC 5 cut(s) 1635, 1756, 1778, 1779, 1951
BmrFI CCNGG 4 cut(s) 371, 759, 1782, 2377
BmrI ACTGGG 1 cut(s) 1752
BmsI GCATC 3 cut(s) 644, 1704, 2311
BmtI GCTAGC 1 cut(s) 1490
BmuI ACTGGG 1 cut(s) 1752
BoxI GACNNNNGTC 1 cut(s) 468
BpiI GAAGAC 1 cut(s) 1219
BpmI CTGGAG 2 cut(s) 23, 693
Bpu10I CCTNAGC 1 cut(s) 27
Bpu1102I GCTNAGC 1 cut(s) 21
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 371
BsaJI CCNNGG 6 cut(s) 204, 757, 1006, 1360, 1780, 1781
BsaWI WCCGGW 1 cut(s) 543
BsaXI ACNNNNNCTCC 2 cut(s) 979, 1009
Bsc4I CCNNNNNNNGG 6 cut(s) 54, 205, 618, 1612, 1763, 1787
Bse1I ACTGG 9 cut(s) 40, 63, 1070, 1223, 1567, 1578, 1758, 1773, 2262
Bse3DI GCAATG 3 cut(s) 497, 1410, 2231
BseBI CCWGG 3 cut(s) 759, 1782, 2377
BseDI CCNNGG 6 cut(s) 204, 757, 1006, 1360, 1780, 1781
BseGI GGATG 5 cut(s) 214, 382, 1634, 2104, 2258
BseLI CCNNNNNNNGG 6 cut(s) 54, 205, 618, 1612, 1763, 1787
BseMI GCAATG 3 cut(s) 497, 1410, 2231
BseMII CTCAG 3 cut(s) 12, 41, 2118
BseNI ACTGG 9 cut(s) 40, 63, 1070, 1223, 1567, 1578, 1758, 1773, 2262
BseRI GAGGAG 2 cut(s) 227, 419
BseXI GCAGC 8 cut(s) 85, 589, 718, 765, 1129, 1234, 1639, 1661
BseYI CCCAGC 1 cut(s) 2324
BsgI GTGCAG 1 cut(s) 243
BshFI GGCC 6 cut(s) 1222, 1365, 1755, 1950, 2118, 2145
BsiHKAI GWGCWC 2 cut(s) 391, 2442
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 3 cut(s) 371, 544, 2062
BslFI GGGAC 4 cut(s) 1048, 1477, 1763, 1898
BslI CCNNNNNNNGG 6 cut(s) 54, 205, 618, 1612, 1763, 1787
BsmFI GGGAC 4 cut(s) 1048, 1477, 1763, 1898
BsmI GAATGC 3 cut(s) 281, 652, 1589
BsnI GGCC 6 cut(s) 1222, 1365, 1755, 1950, 2118, 2145
BsoBI CYCGRG 1 cut(s) 2066
Bsp1286I GDGCHC 2 cut(s) 391, 2442
Bsp1407I TGTACA 4 cut(s) 237, 1081, 1339, 1797
Bsp143I GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
Bsp1720I GCTNAGC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 204
BspACI CCGC 5 cut(s) 274, 425, 738, 1599, 1942
BspANI GGCC 6 cut(s) 1222, 1365, 1755, 1950, 2118, 2145
BspCNI CTCAG 3 cut(s) 13, 40, 2119
BspHI TCATGA 4 cut(s) 1162, 1503, 1515, 2464
BspLI GGNNCC 5 cut(s) 1635, 1756, 1778, 1779, 1951
BspMAI CTGCAG 3 cut(s) 103, 1252, 2185
BspMI ACCTGC 1 cut(s) 1860
BspOI GCTAGC 1 cut(s) 1490
BspPI GGATC 4 cut(s) 192, 749, 1855, 2182
BspQI GCTCTTC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 427
BsrDI GCAATG 3 cut(s) 497, 1410, 2231
BsrGI TGTACA 4 cut(s) 237, 1081, 1339, 1797
BsrI ACTGG 9 cut(s) 40, 63, 1070, 1223, 1567, 1578, 1758, 1773, 2262
BssECI CCNNGG 6 cut(s) 204, 757, 1006, 1360, 1780, 1781
BssMI GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
BssNAI GTATAC 1 cut(s) 1906
BssT1I CCWWGG 2 cut(s) 204, 1006
Bst1107I GTATAC 1 cut(s) 1906
Bst2UI CCWGG 3 cut(s) 759, 1782, 2377
Bst6I CTCTTC 4 cut(s) 39, 549, 1613, 2042
BstAPI GCANNNNNTGC 2 cut(s) 1049, 1256
BstAUI TGTACA 4 cut(s) 237, 1081, 1339, 1797
BstDEI CTNAG 6 cut(s) 21, 27, 227, 1022, 2127, 2420
BstDSI CCRYGG 1 cut(s) 204
BstENI CCTNNNNNAGG 2 cut(s) 1610, 1785
BstF5I GGATG 5 cut(s) 214, 382, 1634, 2104, 2258
BstH2I RGCGCY 1 cut(s) 1534
BstHHI GCGC 1 cut(s) 1533
BstKTI GATC 7 cut(s) 13, 187, 744, 1621, 1863, 2177, 2451
BstMBI GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
BstMWI GCNNNNNNNGC 9 cut(s) 274, 759, 816, 1049, 1058, 1244, 1256, 1265, 1421
BstNI CCWGG 3 cut(s) 759, 1782, 2377
BstNSI RCATGY 5 cut(s) 515, 635, 1969, 2240, 2278
BstPAI GACNNNNGTC 1 cut(s) 468
BstSCI CCNGG 4 cut(s) 369, 757, 1780, 2375
BstSFI CTRYAG 3 cut(s) 99, 1248, 2181
BstV1I GCAGC 8 cut(s) 85, 589, 718, 765, 1129, 1234, 1639, 1661
BstV2I GAAGAC 1 cut(s) 1219
BstXI CCANNNNNNTGG 1 cut(s) 293
BstZ17I GTATAC 1 cut(s) 1906
BsuRI GGCC 6 cut(s) 1222, 1365, 1755, 1950, 2118, 2145
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 5 cut(s) 214, 382, 1634, 2104, 2258
BtsI GCAGTG 1 cut(s) 1496
BtsIMutI CAGTG 8 cut(s) 70, 472, 1496, 1560, 1571, 1908, 2208, 2449
BveI ACCTGC 1 cut(s) 1860
CaiI CAGNNNCTG 2 cut(s) 734, 1037
CciI TCATGA 4 cut(s) 1162, 1503, 1515, 2464
CfoI GCGC 1 cut(s) 1533
Cfr13I GGNCC 4 cut(s) 664, 1754, 1777, 1949
Csp6I GTAC 9 cut(s) 238, 318, 821, 834, 1082, 1147, 1340, 1798, 2104
CviQI GTAC 9 cut(s) 238, 318, 821, 834, 1082, 1147, 1340, 1798, 2104
DdeI CTNAG 6 cut(s) 21, 27, 227, 1022, 2127, 2420
DpnI GATC 7 cut(s) 12, 186, 743, 1620, 1862, 2176, 2450
DpnII GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
DraI TTTAAA 1 cut(s) 976
DraIII CACNNNGTG 1 cut(s) 2446
EaeI YGGCCR 3 cut(s) 1220, 1363, 2116
Eam1104I CTCTTC 4 cut(s) 39, 549, 1613, 2042
EarI CTCTTC 4 cut(s) 39, 549, 1613, 2042
Ecl136II GAGCTC 1 cut(s) 389
Eco130I CCWWGG 2 cut(s) 204, 1006
Eco147I AGGCCT 1 cut(s) 2145
Eco24I GRGCYC 1 cut(s) 391
Eco47I GGWCC 2 cut(s) 664, 1777
Eco53kI GAGCTC 1 cut(s) 389
Eco57I CTGAAG 1 cut(s) 1059
Eco88I CYCGRG 1 cut(s) 2066
EcoICRI GAGCTC 1 cut(s) 389
EcoNI CCTNNNNNAGG 2 cut(s) 1610, 1785
EcoO109I RGGNCCY 1 cut(s) 1777
EcoRI GAATTC 1 cut(s) 58
EcoRII CCWGG 3 cut(s) 757, 1780, 2375
EcoT14I CCWWGG 2 cut(s) 204, 1006
EcoT22I ATGCAT 2 cut(s) 637, 2416
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 2 cut(s) 204, 1006
FaqI GGGAC 4 cut(s) 1048, 1477, 1763, 1898
FauI CCCGC 2 cut(s) 432, 731
FauNDI CATATG 1 cut(s) 598
FbaI TGATCA 2 cut(s) 10, 1618
FblI GTMKAC 1 cut(s) 1905
Fnu4HI GCNGC 9 cut(s) 99, 275, 603, 732, 754, 1143, 1248, 1650, 1653
FokI GGATG 5 cut(s) 221, 389, 1641, 2111, 2245
FriOI GRGCYC 1 cut(s) 391
Fsp4HI GCNGC 9 cut(s) 99, 275, 603, 732, 754, 1143, 1248, 1650, 1653
FspBI CTAG 4 cut(s) 1244, 1326, 1487, 2297
GlaI GCGC 1 cut(s) 1532
GluI GCNGC 9 cut(s) 99, 275, 603, 732, 754, 1143, 1248, 1650, 1653
GsaI CCCAGC 1 cut(s) 2328
GsuI CTGGAG 2 cut(s) 23, 693
HaeII RGCGCY 1 cut(s) 1534
HaeIII GGCC 6 cut(s) 1222, 1365, 1755, 1950, 2118, 2145
HapII CCGG 3 cut(s) 371, 544, 2062
HhaI GCGC 1 cut(s) 1533
Hin6I GCGC 1 cut(s) 1531
HinP1I GCGC 1 cut(s) 1531
HincII GTYRAC 2 cut(s) 45, 2267
HindII GTYRAC 2 cut(s) 45, 2267
HindIII AAGCTT 1 cut(s) 191
HpaII CCGG 3 cut(s) 371, 544, 2062
HphI GGTGA 3 cut(s) 697, 704, 1837
Hpy166II GTNNAC 7 cut(s) 45, 1342, 1798, 1800, 1906, 2206, 2267
Hpy188I TCNGA 4 cut(s) 189, 367, 2092, 2098
Hpy188III TCNNGA 9 cut(s) 129, 916, 1163, 1205, 1379, 1504, 1516, 1552, 2465
Hpy8I GTNNAC 7 cut(s) 45, 1342, 1798, 1800, 1906, 2206, 2267
HpyAV CCTTC 3 cut(s) 662, 1127, 2135
HpyCH4IV ACGT 3 cut(s) 347, 566, 1699
HpyF10VI GCNNNNNNNGC 9 cut(s) 274, 759, 816, 1049, 1058, 1244, 1256, 1265, 1421
HpyF3I CTNAG 6 cut(s) 21, 27, 227, 1022, 2127, 2420
HpySE526I ACGT 3 cut(s) 347, 566, 1699
HspAI GCGC 1 cut(s) 1531
KflI GGGWCCC 1 cut(s) 1777
Ksp22I TGATCA 2 cut(s) 10, 1618
Kzo9I GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 4 cut(s) 386, 432, 865, 2437
Lsp1109I GCAGC 8 cut(s) 85, 589, 718, 765, 1129, 1234, 1639, 1661
LweI GCATC 3 cut(s) 644, 1704, 2311
MaeI CTAG 4 cut(s) 1244, 1326, 1487, 2297
MaeII ACGT 3 cut(s) 347, 566, 1699
MaeIII GTNAC 4 cut(s) 65, 1417, 1569, 1991
MalI GATC 7 cut(s) 12, 186, 743, 1620, 1862, 2176, 2450
MbiI CCGCTC 1 cut(s) 427
MboI GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
MfeI CAATTG 2 cut(s) 2169, 2211
MhlI GDGCHC 2 cut(s) 391, 2442
MlsI TGGCCA 2 cut(s) 1222, 2118
MluNI TGGCCA 2 cut(s) 1222, 2118
MlyI GAGTC 7 cut(s) 1234, 1338, 1501, 2078, 2087, 2201, 2479
MmeI TCCRAC 4 cut(s) 364, 603, 977, 2464
Mox20I TGGCCA 2 cut(s) 1222, 2118
Mph1103I ATGCAT 2 cut(s) 637, 2416
MscI TGGCCA 2 cut(s) 1222, 2118
MseI TTAA 4 cut(s) 887, 975, 1296, 1386
MslI CAYNNNNRTG 4 cut(s) 516, 680, 1415, 2354
Msp20I TGGCCA 2 cut(s) 1222, 2118
MspA1I CMGCKG 4 cut(s) 731, 1079, 1601, 1652
MspI CCGG 3 cut(s) 371, 544, 2062
MspR9I CCNGG 4 cut(s) 371, 759, 1782, 2377
MunI CAATTG 2 cut(s) 2169, 2211
Mva1269I GAATGC 3 cut(s) 281, 652, 1589
MvaI CCWGG 3 cut(s) 759, 1782, 2377
MwoI GCNNNNNNNGC 9 cut(s) 274, 759, 816, 1049, 1058, 1244, 1256, 1265, 1421
NciI CCSGG 1 cut(s) 371
NcoI CCATGG 1 cut(s) 204
NdeI CATATG 1 cut(s) 598
NdeII GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
NheI GCTAGC 1 cut(s) 1486
NlaIV GGNNCC 5 cut(s) 1635, 1756, 1778, 1779, 1951
NmeAIII GCCGAG 1 cut(s) 1341
NmuCI GTSAC 3 cut(s) 65, 1417, 1569
NsiI ATGCAT 2 cut(s) 637, 2416
NspI RCATGY 5 cut(s) 515, 635, 1969, 2240, 2278
OliI CACNNNNGTG 1 cut(s) 2354
PaeI GCATGC 3 cut(s) 635, 2240, 2278
PaeR7I CTCGAG 1 cut(s) 2066
PagI TCATGA 4 cut(s) 1162, 1503, 1515, 2464
PasI CCCWGGG 1 cut(s) 1781
PceI AGGCCT 1 cut(s) 2145
PciI ACATGT 1 cut(s) 1965
PciSI GCTCTTC 1 cut(s) 549
PctI GAATGC 3 cut(s) 281, 652, 1589
PfeI GAWTC 8 cut(s) 104, 700, 1393, 1402, 1428, 1543, 1929, 2372
PflMI CCANNNNNTGG 2 cut(s) 205, 618
PkrI GCNGC 9 cut(s) 100, 276, 604, 733, 755, 1144, 1249, 1651, 1654
PleI GAGTC 7 cut(s) 1234, 1337, 1501, 2077, 2087, 2201, 2478
PpsI GAGTC 7 cut(s) 1234, 1337, 1501, 2077, 2087, 2201, 2478
PpuMI RGGWCCY 1 cut(s) 1777
PscI ACATGT 1 cut(s) 1965
PshAI GACNNNNGTC 1 cut(s) 468
PshBI ATTAAT 1 cut(s) 1386
Psp124BI GAGCTC 1 cut(s) 391
Psp1406I AACGTT 1 cut(s) 1699
Psp5II RGGWCCY 1 cut(s) 1777
Psp6I CCWGG 3 cut(s) 757, 1780, 2375
PspFI CCCAGC 1 cut(s) 2324
PspGI CCWGG 3 cut(s) 757, 1780, 2375
PspN4I GGNNCC 5 cut(s) 1635, 1756, 1778, 1779, 1951
PspPI GGNCC 4 cut(s) 664, 1754, 1777, 1949
PspPPI RGGWCCY 1 cut(s) 1777
PspXI VCTCGAGB 1 cut(s) 2066
PstI CTGCAG 3 cut(s) 103, 1252, 2185
PstNI CAGNNNCTG 2 cut(s) 734, 1037
PvuII CAGCTG 3 cut(s) 731, 1079, 1652
RsaI GTAC 9 cut(s) 239, 319, 822, 835, 1083, 1148, 1341, 1799, 2105
RsaNI GTAC 9 cut(s) 238, 318, 821, 834, 1082, 1147, 1340, 1798, 2104
RseI CAYNNNNRTG 4 cut(s) 516, 680, 1415, 2354
SacI GAGCTC 1 cut(s) 391
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 4 cut(s) 887, 975, 1296, 1386
SatI GCNGC 9 cut(s) 99, 275, 603, 732, 754, 1143, 1248, 1650, 1653
Sau3AI GATC 7 cut(s) 10, 184, 741, 1618, 1860, 2174, 2448
Sau96I GGNCC 4 cut(s) 664, 1754, 1777, 1949
SchI GAGTC 7 cut(s) 1234, 1338, 1501, 2078, 2087, 2201, 2479
ScrFI CCNGG 4 cut(s) 371, 759, 1782, 2377
SduI GDGCHC 2 cut(s) 391, 2442
SfaNI GCATC 3 cut(s) 644, 1704, 2311
SfcI CTRYAG 3 cut(s) 99, 1248, 2181
Sfr274I CTCGAG 1 cut(s) 2066
SinI GGWCC 2 cut(s) 664, 1777
SlaI CTCGAG 1 cut(s) 2066
SmiMI CAYNNNNRTG 4 cut(s) 516, 680, 1415, 2354
SmlI CTYRAG 2 cut(s) 431, 2066
SmoI CTYRAG 2 cut(s) 431, 2066
SphI GCATGC 3 cut(s) 635, 2240, 2278
SseBI AGGCCT 1 cut(s) 2145
SsiI CCGC 5 cut(s) 274, 425, 738, 1599, 1942
SspI AATATT 1 cut(s) 801
SspMI CTAG 4 cut(s) 1244, 1326, 1487, 2297
SstI GAGCTC 1 cut(s) 391
StuI AGGCCT 1 cut(s) 2145
StyD4I CCNGG 4 cut(s) 369, 757, 1780, 2375
StyI CCWWGG 2 cut(s) 204, 1006
TaiI ACGT 3 cut(s) 350, 569, 1702
TaqI TCGA 3 cut(s) 2040, 2067, 2177
TatI WGTACW 5 cut(s) 237, 1081, 1339, 1797, 2103
TauI GCSGC 1 cut(s) 277
TfiI GAWTC 8 cut(s) 104, 700, 1393, 1402, 1428, 1543, 1929, 2372
Tru1I TTAA 4 cut(s) 887, 975, 1296, 1386
Tru9I TTAA 4 cut(s) 887, 975, 1296, 1386
TscAI CASTG 8 cut(s) 70, 472, 1503, 1567, 1578, 1915, 2208, 2449
TseFI GTSAC 3 cut(s) 65, 1417, 1569
TseI GCWGC 8 cut(s) 98, 602, 731, 753, 1142, 1247, 1649, 1652
Tsp45I GTSAC 3 cut(s) 65, 1417, 1569
TspGWI ACGGA 1 cut(s) 1961
TspRI CASTG 8 cut(s) 70, 472, 1503, 1567, 1578, 1915, 2208, 2449
Van91I CCANNNNNTGG 2 cut(s) 205, 618
VpaK11BI GGWCC 2 cut(s) 664, 1777
VspI ATTAAT 1 cut(s) 1386
XagI CCTNNNNNAGG 2 cut(s) 1610, 1785
XapI RAATTY 5 cut(s) 58, 71, 114, 360, 2300
XceI RCATGY 5 cut(s) 515, 635, 1969, 2240, 2278
XcmI CCANNNNNNNNNTGG 3 cut(s) 619, 1386, 1765
XhoI CTCGAG 1 cut(s) 2066
XmiI GTMKAC 1 cut(s) 1905
XspI CTAG 4 cut(s) 1244, 1326, 1487, 2297
Zsp2I ATGCAT 2 cut(s) 637, 2416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.