Rorug05G0059600

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000477
Physical Location & Seq
Reverse (-)
5202488 .. 5205592
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0059600.1

Sequence Viewer

Length: 2859 bp
ATGGAGACCAATTTAAAATCTAGGGTTGGGGCTCTTCCAATTTCCCCAACCACTCCTCTACAAACCCATAAATCCCTACTCACTCCCCTCCTCCTCCTCCTCTTCTTCTTCTTCATCAATTTCACCCCCACAGCTTCAACCCTGAACCAAATCCCCAATCTTGATCAACTATACTCCAGACACTGCAACGACGTCGTTCCCAAATCGGAGACGCACCCCAAGTGGTTCCTCACCACCCTCTCCAGCCAAGACCTCGGATTCCACAGCAGTTACTTCACCGGCGGCGATCGGCTTTTCAATCAGAGCTCCGGCGGCCGCAAAGCTCTCATCTTCCGGCCCAACTACGTAGCTCGAACCCTAGCCGATGGAATTTTCCATGTCAAAGGAAGTATGGATCTTCAATCTCTTCTGGGCTATTCGACTCGCCGGAATCTGAAGTTGGTTAAGCCCAGAATTCCGGTCCGGCGGGGCGTAGTGAGGTTTAGGCTTGATGGGTATTATTCAGAATCGTCAAAGAAGCTGTGTATGATTGGGAAAGTTATGTCCATGTATAATGCAGGTAAGTTTGAGGCTAGTGTTGTTCTTAAGCTTAATTATCCGAAATGGTCTAGTGTTTATGATAGTTTGATTACTGGGAGTTTAGAGAGCGTTGTTATTGCTGAGGGCGATGATGGTAAGAATTATTTTGATCCCGTTTTGATGTTGGGTTTGTCTGTGAAACGTAATTATGAGTATACATTTGTTGGGAAAGATAGGCTGAGTAGTTTTCCGAGTAGTGTTGATAGAGGAGAGAGTATAGCTTTGGAGAATTTGAATGATTCGAGACGTGATTTGTGTAGGTTGCTTGGCAACCGTCTTGAGAGATATGAATTGGTGTATGGGAGTGAGTGTGGGGGTGTGAATTGCAATCCTGTTGGTGGGGATGTTGGATATGTACCTGGCTCGTTGGTTTTTTATGGGACTCGGTGTACAGAAGGGAGGAAAGTGCAGATGTTGTTGGGGTTTTCCAACTCCAGTGTAGATGAATACATAAGATTTCCATTTGTGCCTAGCACGACGTTCATTGCTGAAGGGGAATGGGATGAGAAGGGGAATCGTCTATGCGCGGTTGCGTGTAGGATTTTGAATTTTACAGAATCTTTGACTAGTGCTCGTGTTGGAGACTGCTCAACTAAACTCAGCTTGAGATTTCCAGTAAAGTTGTCTTTACAAAATAGGTCTGCTGTTGTGGGGCAAATTTGGAGCGACAAAAAAGCGAGTGATCCAGGTTACTTCAGTAAAATTGGGTTCCAAGGTTTGTCGGGATGGTTAGTGAAACTCTTAGGATACAAATATGAATATTCTGTGAACGACAACCTGAGAAAAACATGTGCGGAAAAGAGGGCTGGGAGAGGCAAAGGAAAGCAATATCCTGAGGAGTATTCATCAGATCTGAAATTTGACATGAGAGTGAGCAACAGCAAAGGACAAGTTGCATGGGGTTATTCATCGCCACTATTTGTTGATGATGACCGGATTTATGGACGCCGGTTTTGGGATAAACCACAGGAAACAGAAGTACCAAGTCGTCGGAAGAAAAGCCATAGCAGTCCGATGAACATTACCTATAAGTTGAGCTTCACACGTGGTGTTCAATTTTTTCATGATGTCTTCCCATCTAAAGCAGAACTAGCTGCTGAAGGAATATATGATAGAGATTATGGAAACCTATGCATGATAGGATGTAGGCATGTACCATCCAAGGAACAAAACTCGATTAAAAAGGATATGTTAGACTGTTCTGTTAAAATTGATGTACACTTTCCTCCATTGGATACCAAGGATGGCCAGAATGTCAAGGGAACTATTGAAAGCACGAGGAATAAGTCAGACCCTCTTTATTTTGAACGTATTGACTTCTCTTCGACTTCAATTTACCATGACCAAGCTGCTGCATCCATTACGAGAATCGATTTTGAGATTGTTATGGTTTTGATTTCCAATACACTTGCATGTGCTTTTGTCGGATGGCAACTCTATTTTGTGAAGAAAAACCCAGATGTGCTCCCATTCACTTCAATTGTCATGCTCATTGTTCTTACTCTGGGATACGTGATTCCTCTTCTGTTCAACTTTGAAGCAATGCTTGCACCAAACCAAAGCAAGCAGAGTACTTTTCTTGGGATTGGTGGATGGCTCCAGGTTAATGAAGTAATGGTCAGGGTGATAATGTTGGTAACTTTCATTTTGCAGCTGCTTCTTGTCCAACTGACCTGGTCATCGAGACAATGTGATAATAGCCAGAAGAGCTTGATGGATTCTGAGAGGAAGGTTCTTTATGCCACCTTACTATTGTACATAGCTGGTGCACTGATTACTTGCTCTGTCCACCAGTGGATGAGTACTCACCAGACCTCTTACAGGCCATTTCATCGCAAGCTTCGGCGAGTGGCTAACCTATGGCATCTTGTTCACCCGCAACGCTCTCTCTGGGACGACCTCAAGTCTTATGCTGGATTCATCCTGGACGGTTTTCTACTCCCGCAAATATTGTTCAACTTATTCATCAATTCAGGTGAAAAGGCTCTGTCCTGTGCCTTTTACTTTGGAACCACCATGATTCGTCTGCTGCCTCATGCTTATGATCTGTACAGAGCTCGCGCTTACACATGGTTACTTGCTTTCTCATATATTTATGGAAACCCCAAAACCGATTTTTTCTCCACTGCCTGGAACATAATCATACCCTGTTGTTGTCTGCTGCTTGCTGCCATTATTTTCTTGCAGCAAAGGTTTGGCGGCCGTTGCATCCTTCCCAAAATATTTGGGCATTCCACTGTATATGAAAAGGTCCCTGTCATCAGCAATGAGGAATTGTGA

Protein Analysis

952

Amino Acids

107.96

Weight (kDa)

9.19

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2921_N PF25333 58 - 232 1.9e-18 DUF2921 lipocalin-like domain
DUF2921_N PF25333 256 - 428 1.9e-30 DUF2921 lipocalin-like domain
DUF2921_N PF25333 459 - 632 1.8e-33 DUF2921 lipocalin-like domain
DUF2921 PF11145 643 - 935 1.7e-57 Transmembrane E3 ligase/DUF2921 transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000477)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 195
Acc36I ACCTGC 1 cut(s) 548
AccB7I CCANNNNNTGG 1 cut(s) 2709
AccI GTMKAC 1 cut(s) 734
AccII CGCG 2 cut(s) 1106, 2640
AciI CCGC 9 cut(s) 282, 312, 316, 466, 1106, 1373, 2456, 2522, 2778
AclWI GGATC 3 cut(s) 402, 683, 1256
AcoI YGGCCR 3 cut(s) 313, 1825, 2779
AcsI RAATTY 6 cut(s) 369, 453, 808, 1126, 1236, 1436
AcuI CTGAAG 4 cut(s) 455, 1089, 1258, 1698
AcvI CACGTG 1 cut(s) 1625
AcyI GRCGYC 2 cut(s) 192, 1525
AdeI CACNNNGTG 1 cut(s) 1628
AfaI GTAC 9 cut(s) 936, 970, 1560, 1734, 1797, 2152, 2336, 2383, 2630
AfiI CCNNNNNNNGG 4 cut(s) 917, 1534, 2400, 2709
AflII CTTAAG 1 cut(s) 584
AflIII ACRYGT 2 cut(s) 1367, 1622
AhdI GACNNNNNGTC 1 cut(s) 2482
AhlI ACTAGT 1 cut(s) 1145
AjiI CACGTC 1 cut(s) 827
AjnI CCWGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
Alw21I GWGCWC 5 cut(s) 308, 1153, 2046, 2350, 2638
Alw26I GTCTC 4 cut(s) 203, 817, 1155, 2257
Alw44I GTGCAC 1 cut(s) 2346
AlwI GGATC 3 cut(s) 402, 683, 1256
AlwNI CAGNNNCTG 1 cut(s) 183
AoxI GGCC 5 cut(s) 313, 335, 1825, 2402, 2779
ApaLI GTGCAC 1 cut(s) 2346
ApeKI GCWGC 9 cut(s) 1673, 1928, 1931, 2230, 2233, 2608, 2740, 2747, 2764
ApoI RAATTY 6 cut(s) 369, 453, 808, 1126, 1236, 1436
ArsI GACNNNNNNTTYG 2 cut(s) 1515, 1547
AspLEI GCGC 2 cut(s) 1106, 2642
AspS9I GGNCC 3 cut(s) 336, 460, 2830
AsuHPI GGTGA 7 cut(s) 115, 223, 268, 2215, 2378, 2444, 2567
AvaII GGWCC 2 cut(s) 460, 2830
AxyI CCTNAGG 1 cut(s) 1413
BaeGI GKGCMC 1 cut(s) 2350
BaeI ACNNNNGTAYC 2 cut(s) 1542, 1575
BalI TGGCCA 1 cut(s) 1827
BanII GRGCYC 3 cut(s) 34, 308, 2638
BauI CACGAG 2 cut(s) 1152, 1855
BbrPI CACGTG 1 cut(s) 1625
BbsI GAAGAC 1 cut(s) 1642
Bbv12I GWGCWC 5 cut(s) 308, 1153, 2046, 2350, 2638
BbvCI CCTCAGC 1 cut(s) 660
BbvI GCAGC 9 cut(s) 1660, 1915, 1918, 2220, 2242, 2595, 2727, 2734, 2776
BceAI ACGGC 1 cut(s) 2766
BcgI CGANNNNNNTGC 2 cut(s) 175, 209
BciT130I CCWGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
BciVI GTATCC 3 cut(s) 1319, 1807, 2081
BclI TGATCA 1 cut(s) 163
BcoDI GTCTC 4 cut(s) 203, 817, 1155, 2257
BcuI ACTAGT 1 cut(s) 1145
BfaI CTAG 7 cut(s) 21, 359, 573, 609, 1050, 1146, 1670
BfrI CTTAAG 1 cut(s) 584
BfuAI ACCTGC 1 cut(s) 548
BfuI GTATCC 3 cut(s) 1319, 1807, 2081
BglII AGATCT 1 cut(s) 1429
BmcAI AGTACT 2 cut(s) 2152, 2383
Bme1390I CCNGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
Bme18I GGWCC 2 cut(s) 460, 2830
BmeRI GACNNNNNGTC 1 cut(s) 2482
BmgBI CACGTC 1 cut(s) 827
BmgT120I GGNCC 3 cut(s) 336, 460, 2830
BmiI GGNNCC 5 cut(s) 227, 1289, 2177, 2590, 2832
BmrFI CCNGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
BmrI ACTGGG 1 cut(s) 642
BmsI GCATC 3 cut(s) 1943, 2452, 2796
BmuI ACTGGG 1 cut(s) 642
BpiI GAAGAC 1 cut(s) 1642
BpmI CTGGAG 4 cut(s) 160, 226, 997, 2162
Bpu10I CCTNAGC 1 cut(s) 660
BpuEI CTTGAG 3 cut(s) 878, 1204, 2465
Bsa29I ATCGAT 1 cut(s) 1950
BsaAI YACGTR 3 cut(s) 346, 1625, 2092
BsaHI GRCGYC 2 cut(s) 192, 1525
BsaJI CCNNGG 4 cut(s) 253, 1291, 1740, 1818
BsaWI WCCGGW 2 cut(s) 457, 1512
BsaXI ACNNNNNCTCC 2 cut(s) 874, 904
Bsc4I CCNNNNNNNGG 4 cut(s) 917, 1534, 2400, 2709
Bse118I RCCGGY 2 cut(s) 278, 1527
Bse1I ACTGG 4 cut(s) 637, 1014, 1193, 2371
Bse21I CCTNAGG 1 cut(s) 1413
Bse3DI GCAATG 3 cut(s) 1062, 2127, 2851
BseBI CCWGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
BseCI ATCGAT 1 cut(s) 1950
BseDI CCNNGG 4 cut(s) 253, 1291, 1740, 1818
BseLI CCNNNNNNNGG 4 cut(s) 917, 1534, 2400, 2709
BseMI GCAATG 3 cut(s) 1062, 2127, 2851
BseMII CTCAG 6 cut(s) 651, 749, 1192, 1349, 1404, 2292
BseNI ACTGG 4 cut(s) 637, 1014, 1193, 2371
BseRI GAGGAG 7 cut(s) 45, 80, 83, 86, 89, 801, 1430
BseSI GKGCMC 1 cut(s) 2350
BseX3I CGGCCG 2 cut(s) 313, 2779
BseXI GCAGC 9 cut(s) 1660, 1915, 1918, 2220, 2242, 2595, 2727, 2734, 2776
BseYI CCCAGC 1 cut(s) 1385
BsgI GTGCAG 1 cut(s) 1007
Bsh1236I CGCG 2 cut(s) 1106, 2640
Bsh1285I CGRYCG 3 cut(s) 289, 316, 2782
BshFI GGCC 5 cut(s) 315, 337, 1827, 2404, 2781
BshVI ATCGAT 1 cut(s) 1950
BsiEI CGRYCG 3 cut(s) 289, 316, 2782
BsiHKAI GWGCWC 5 cut(s) 308, 1153, 2046, 2350, 2638
BsiSI CCGG 8 cut(s) 279, 309, 334, 427, 458, 463, 1513, 1528
BslFI GGGAC 3 cut(s) 973, 2486, 2816
BslI CCNNNNNNNGG 4 cut(s) 917, 1534, 2400, 2709
BsmAI GTCTC 4 cut(s) 203, 817, 1155, 2257
BsmBI CGTCTC 2 cut(s) 203, 817
BsmFI GGGAC 3 cut(s) 973, 2486, 2816
BsmI GAATGC 1 cut(s) 2809
BsnI GGCC 5 cut(s) 315, 337, 1827, 2404, 2781
Bsp1286I GDGCHC 6 cut(s) 34, 308, 1153, 2046, 2350, 2638
Bsp1407I TGTACA 4 cut(s) 968, 1795, 2334, 2628
Bsp143I GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
BspACI CCGC 9 cut(s) 282, 312, 316, 466, 1106, 1373, 2456, 2522, 2778
BspANI GGCC 5 cut(s) 315, 337, 1827, 2404, 2781
BspCNI CTCAG 6 cut(s) 652, 750, 1191, 1350, 1405, 2293
BspDI ATCGAT 1 cut(s) 1950
BspFNI CGCG 2 cut(s) 1106, 2640
BspHI TCATGA 1 cut(s) 1642
BspLI GGNNCC 5 cut(s) 227, 1289, 2177, 2590, 2832
BspMI ACCTGC 1 cut(s) 548
BspPI GGATC 3 cut(s) 402, 683, 1256
BspQI GCTCTTC 2 cut(s) 39, 2279
BspTI CTTAAG 1 cut(s) 584
BsrDI GCAATG 3 cut(s) 1062, 2127, 2851
BsrFI RCCGGY 2 cut(s) 278, 1527
BsrGI TGTACA 4 cut(s) 968, 1795, 2334, 2628
BsrI ACTGG 4 cut(s) 637, 1014, 1193, 2371
BssAI RCCGGY 2 cut(s) 278, 1527
BssECI CCNNGG 4 cut(s) 253, 1291, 1740, 1818
BssMI GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
BssNAI GTATAC 1 cut(s) 735
BssNI GRCGYC 2 cut(s) 192, 1525
BssSI CACGAG 2 cut(s) 1152, 1855
BssT1I CCWWGG 3 cut(s) 1291, 1740, 1818
Bst1107I GTATAC 1 cut(s) 735
Bst2BI CACGAG 2 cut(s) 1152, 1855
Bst2UI CCWGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
Bst4CI ACNGT 4 cut(s) 854, 1778, 2510, 2818
Bst6I CTCTTC 6 cut(s) 39, 107, 411, 1906, 2106, 2279
BstACI GRCGYC 2 cut(s) 192, 1525
BstAFI CTTAAG 1 cut(s) 584
BstAPI GCANNNNNTGC 1 cut(s) 2126
BstAUI TGTACA 4 cut(s) 968, 1795, 2334, 2628
BstBAI YACGTR 3 cut(s) 346, 1625, 2092
BstC8I GCNNGC 5 cut(s) 2127, 2144, 2417, 2638, 2745
BstDEI CTNAG 7 cut(s) 660, 758, 1178, 1321, 1358, 1413, 2301
BstENI CCTNNNNNAGG 1 cut(s) 2398
BstFNI CGCG 2 cut(s) 1106, 2640
BstHHI GCGC 2 cut(s) 1106, 2642
BstKTI GATC 7 cut(s) 166, 289, 397, 691, 1264, 1432, 2626
BstMAI GTCTC 4 cut(s) 203, 817, 1155, 2257
BstMBI GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
BstMCI CGRYCG 3 cut(s) 289, 316, 2782
BstMWI GCNNNNNNNGC 5 cut(s) 312, 1670, 2126, 2286, 2784
BstNI CCWGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
BstNSI RCATGY 3 cut(s) 1371, 1733, 1995
BstSCI CCNGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
BstSLI GKGCMC 1 cut(s) 2350
BstSNI TACGTA 1 cut(s) 346
BstUI CGCG 2 cut(s) 1106, 2640
BstV1I GCAGC 9 cut(s) 1660, 1915, 1918, 2220, 2242, 2595, 2727, 2734, 2776
BstV2I GAAGAC 1 cut(s) 1642
BstX2I RGATCY 2 cut(s) 394, 1429
BstYI RGATCY 2 cut(s) 394, 1429
BstZ17I GTATAC 1 cut(s) 735
BstZI CGGCCG 2 cut(s) 313, 2779
Bsu15I ATCGAT 1 cut(s) 1950
Bsu36I CCTNAGG 1 cut(s) 1413
BsuI GTATCC 3 cut(s) 1319, 1807, 2081
BsuRI GGCC 5 cut(s) 315, 337, 1827, 2404, 2781
BsuTUI ATCGAT 1 cut(s) 1950
BtgZI GCGATG 3 cut(s) 681, 1473, 2396
BtrI CACGTC 1 cut(s) 827
BtsI GCAGTG 2 cut(s) 181, 2703
BtsIMutI CAGTG 6 cut(s) 181, 1021, 2348, 2378, 2703, 2814
BveI ACCTGC 1 cut(s) 548
Cac8I GCNNGC 5 cut(s) 2127, 2144, 2417, 2638, 2745
CaiI CAGNNNCTG 1 cut(s) 183
CciI TCATGA 1 cut(s) 1642
CciNI GCGGCCGC 1 cut(s) 313
CfoI GCGC 2 cut(s) 1106, 2642
Cfr10I RCCGGY 2 cut(s) 278, 1527
Cfr13I GGNCC 3 cut(s) 336, 460, 2830
ClaI ATCGAT 1 cut(s) 1950
CpoI CGGWCCG 1 cut(s) 460
CseI GACGC 2 cut(s) 220, 1533
CsiI ACCWGGT 1 cut(s) 2252
Csp6I GTAC 9 cut(s) 935, 969, 1559, 1733, 1796, 2151, 2335, 2382, 2629
CspI CGGWCCG 1 cut(s) 460
CviQI GTAC 9 cut(s) 935, 969, 1559, 1733, 1796, 2151, 2335, 2382, 2629
DdeI CTNAG 7 cut(s) 660, 758, 1178, 1321, 1358, 1413, 2301
DpnI GATC 7 cut(s) 165, 288, 396, 690, 1263, 1431, 2625
DpnII GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
DraI TTTAAA 1 cut(s) 15
DraIII CACNNNGTG 1 cut(s) 1628
DriI GACNNNNNGTC 1 cut(s) 2482
EaeI YGGCCR 3 cut(s) 313, 1825, 2779
EagI CGGCCG 2 cut(s) 313, 2779
Eam1104I CTCTTC 6 cut(s) 39, 107, 411, 1906, 2106, 2279
Eam1105I GACNNNNNGTC 1 cut(s) 2482
EarI CTCTTC 6 cut(s) 39, 107, 411, 1906, 2106, 2279
Ecl136II GAGCTC 2 cut(s) 306, 2636
EclXI CGGCCG 2 cut(s) 313, 2779
Eco105I TACGTA 1 cut(s) 346
Eco130I CCWWGG 3 cut(s) 1291, 1740, 1818
Eco24I GRGCYC 3 cut(s) 34, 308, 2638
Eco47I GGWCC 2 cut(s) 460, 2830
Eco52I CGGCCG 2 cut(s) 313, 2779
Eco53kI GAGCTC 2 cut(s) 306, 2636
Eco57I CTGAAG 4 cut(s) 455, 1089, 1258, 1698
Eco72I CACGTG 1 cut(s) 1625
Eco81I CCTNAGG 1 cut(s) 1413
EcoICRI GAGCTC 2 cut(s) 306, 2636
EcoNI CCTNNNNNAGG 1 cut(s) 2398
EcoO109I RGGNCCY 1 cut(s) 2830
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
EcoT14I CCWWGG 3 cut(s) 1291, 1740, 1818
EcoT22I ATGCAT 1 cut(s) 1715
EcoT38I GRGCYC 3 cut(s) 34, 308, 2638
ErhI CCWWGG 3 cut(s) 1291, 1740, 1818
Esp3I CGTCTC 2 cut(s) 203, 817
FalI AAGNNNNNCTT 4 cut(s) 1601, 1633, 2109, 2141
FaqI GGGAC 3 cut(s) 973, 2486, 2816
FauI CCCGC 3 cut(s) 459, 2463, 2529
FbaI TGATCA 1 cut(s) 163
FblI GTMKAC 1 cut(s) 734
FriOI GRGCYC 3 cut(s) 34, 308, 2638
FspBI CTAG 7 cut(s) 21, 359, 573, 609, 1050, 1146, 1670
GlaI GCGC 2 cut(s) 1105, 2641
GsaI CCCAGC 1 cut(s) 1389
GsuI CTGGAG 4 cut(s) 160, 226, 997, 2162
HaeIII GGCC 5 cut(s) 315, 337, 1827, 2404, 2781
HapII CCGG 8 cut(s) 279, 309, 334, 427, 458, 463, 1513, 1528
HgaI GACGC 2 cut(s) 220, 1533
HhaI GCGC 2 cut(s) 1106, 2642
Hin1I GRCGYC 2 cut(s) 192, 1525
Hin6I GCGC 2 cut(s) 1104, 2640
HinP1I GCGC 2 cut(s) 1104, 2640
HindIII AAGCTT 2 cut(s) 587, 2417
HpaII CCGG 8 cut(s) 279, 309, 334, 427, 458, 463, 1513, 1528
HphI GGTGA 7 cut(s) 115, 223, 268, 2215, 2378, 2444, 2567
Hpy166II GTNNAC 7 cut(s) 735, 969, 1348, 1798, 2348, 2368, 2452
Hpy188III TCNNGA 8 cut(s) 161, 177, 822, 857, 1302, 1412, 1643, 2262
Hpy8I GTNNAC 7 cut(s) 735, 969, 1348, 1798, 2348, 2368, 2452
Hpy99I CGWCG 4 cut(s) 194, 197, 1060, 1572
HpyAV CCTTC 6 cut(s) 968, 1064, 1081, 1673, 2302, 2801
HpyCH4III ACNGT 4 cut(s) 854, 1778, 2510, 2818
HpyCH4IV ACGT 8 cut(s) 192, 345, 721, 826, 1058, 1624, 1888, 2091
HpyF10VI GCNNNNNNNGC 5 cut(s) 312, 1670, 2126, 2286, 2784
HpyF3I CTNAG 7 cut(s) 660, 758, 1178, 1321, 1358, 1413, 2301
HpySE526I ACGT 8 cut(s) 192, 345, 721, 826, 1058, 1624, 1888, 2091
Hsp92I GRCGYC 2 cut(s) 192, 1525
HspAI GCGC 2 cut(s) 1104, 2640
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
LguI GCTCTTC 2 cut(s) 39, 2279
LmnI GCTCC 4 cut(s) 311, 1242, 2049, 2181
Lsp1109I GCAGC 9 cut(s) 1660, 1915, 1918, 2220, 2242, 2595, 2727, 2734, 2776
LweI GCATC 3 cut(s) 1943, 2452, 2796
MabI ACCWGGT 1 cut(s) 2252
MaeI CTAG 7 cut(s) 21, 359, 573, 609, 1050, 1146, 1670
MaeII ACGT 8 cut(s) 192, 345, 721, 826, 1058, 1624, 1888, 2091
MaeIII GTNAC 4 cut(s) 269, 1268, 2215, 2652
MalI GATC 7 cut(s) 165, 288, 396, 690, 1263, 1431, 2625
MboI GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
MfeI CAATTG 1 cut(s) 2058
MflI RGATCY 2 cut(s) 394, 1429
MhlI GDGCHC 6 cut(s) 34, 308, 1153, 2046, 2350, 2638
MlsI TGGCCA 1 cut(s) 1827
MluNI TGGCCA 1 cut(s) 1827
MlyI GAGTC 2 cut(s) 415, 955
MmeI TCCRAC 6 cut(s) 907, 1032, 1138, 1550, 1984, 2269
Mox20I TGGCCA 1 cut(s) 1827
Mph1103I ATGCAT 1 cut(s) 1715
MscI TGGCCA 1 cut(s) 1827
MseI TTAA 7 cut(s) 14, 444, 585, 591, 1758, 1785, 2184
MslI CAYNNNNRTG 3 cut(s) 1448, 1990, 2619
Msp20I TGGCCA 1 cut(s) 1827
MspA1I CMGCKG 1 cut(s) 2233
MspCI CTTAAG 1 cut(s) 584
MspI CCGG 8 cut(s) 279, 309, 334, 427, 458, 463, 1513, 1528
MspR9I CCNGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
MunI CAATTG 1 cut(s) 2058
Mva1269I GAATGC 1 cut(s) 2809
MvaI CCWGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
MvnI CGCG 2 cut(s) 1106, 2640
MwoI GCNNNNNNNGC 5 cut(s) 312, 1670, 2126, 2286, 2784
NdeII GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
NlaIV GGNNCC 5 cut(s) 227, 1289, 2177, 2590, 2832
NotI GCGGCCGC 1 cut(s) 313
NsiI ATGCAT 1 cut(s) 1715
NspI RCATGY 3 cut(s) 1371, 1733, 1995
PagI TCATGA 1 cut(s) 1642
PciI ACATGT 1 cut(s) 1367
PciSI GCTCTTC 2 cut(s) 39, 2279
PctI GAATGC 1 cut(s) 2809
PflFI GACNNNGTC 1 cut(s) 2254
PflMI CCANNNNNTGG 1 cut(s) 2709
PfoI TCCNGGA 1 cut(s) 2502
Ple19I CGATCG 1 cut(s) 289
PleI GAGTC 2 cut(s) 415, 955
PmaCI CACGTG 1 cut(s) 1625
PmlI CACGTG 1 cut(s) 1625
PpsI GAGTC 2 cut(s) 415, 955
Ppu21I YACGTR 3 cut(s) 346, 1625, 2092
PpuMI RGGWCCY 1 cut(s) 2830
PscI ACATGT 1 cut(s) 1367
Psp124BI GAGCTC 2 cut(s) 308, 2638
Psp5II RGGWCCY 1 cut(s) 2830
Psp6I CCWGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
PspCI CACGTG 1 cut(s) 1625
PspFI CCCAGC 1 cut(s) 1385
PspGI CCWGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
PspN4I GGNNCC 5 cut(s) 227, 1289, 2177, 2590, 2832
PspPI GGNCC 3 cut(s) 336, 460, 2830
PspPPI RGGWCCY 1 cut(s) 2830
PstNI CAGNNNCTG 1 cut(s) 183
PsuI RGATCY 2 cut(s) 394, 1429
PsyI GACNNNGTC 1 cut(s) 2254
PvuI CGATCG 1 cut(s) 289
PvuII CAGCTG 1 cut(s) 2233
RsaI GTAC 9 cut(s) 936, 970, 1560, 1734, 1797, 2152, 2336, 2383, 2630
RsaNI GTAC 9 cut(s) 935, 969, 1559, 1733, 1796, 2151, 2335, 2382, 2629
RseI CAYNNNNRTG 3 cut(s) 1448, 1990, 2619
Rsr2I CGGWCCG 1 cut(s) 460
RsrII CGGWCCG 1 cut(s) 460
SacI GAGCTC 2 cut(s) 308, 2638
SapI GCTCTTC 2 cut(s) 39, 2279
SaqAI TTAA 7 cut(s) 14, 444, 585, 591, 1758, 1785, 2184
Sau3AI GATC 7 cut(s) 163, 286, 394, 688, 1261, 1429, 2623
Sau96I GGNCC 3 cut(s) 336, 460, 2830
ScaI AGTACT 2 cut(s) 2152, 2383
SchI GAGTC 2 cut(s) 415, 955
ScrFI CCNGG 6 cut(s) 939, 1266, 2180, 2254, 2504, 2710
SduI GDGCHC 6 cut(s) 34, 308, 1153, 2046, 2350, 2638
SexAI ACCWGGT 1 cut(s) 2252
SfaNI GCATC 3 cut(s) 1943, 2452, 2796
SgrAI CRCCGGYG 1 cut(s) 278
SinI GGWCC 2 cut(s) 460, 2830
SmiMI CAYNNNNRTG 3 cut(s) 1448, 1990, 2619
SmlI CTYRAG 4 cut(s) 584, 857, 1183, 2480
SmoI CTYRAG 4 cut(s) 584, 857, 1183, 2480
SnaBI TACGTA 1 cut(s) 346
SpeI ACTAGT 1 cut(s) 1145
SsiI CCGC 9 cut(s) 282, 312, 316, 466, 1106, 1373, 2456, 2522, 2778
SspI AATATT 3 cut(s) 1340, 2529, 2802
SspMI CTAG 7 cut(s) 21, 359, 573, 609, 1050, 1146, 1670
SstI GAGCTC 2 cut(s) 308, 2638
StyD4I CCNGG 6 cut(s) 937, 1264, 2178, 2252, 2502, 2708
StyI CCWWGG 3 cut(s) 1291, 1740, 1818
TaaI ACNGT 4 cut(s) 854, 1778, 2510, 2818
TaiI ACGT 8 cut(s) 195, 348, 724, 829, 1061, 1627, 1891, 2094
TaqI TCGA 7 cut(s) 352, 419, 821, 1754, 1904, 1950, 2261
TatI WGTACW 6 cut(s) 968, 1795, 2150, 2334, 2381, 2628
TauI GCSGC 4 cut(s) 285, 315, 318, 2781
Tru1I TTAA 7 cut(s) 14, 444, 585, 591, 1758, 1785, 2184
Tru9I TTAA 7 cut(s) 14, 444, 585, 591, 1758, 1785, 2184
TscAI CASTG 6 cut(s) 188, 1021, 2355, 2378, 2710, 2821
TseI GCWGC 9 cut(s) 1673, 1928, 1931, 2230, 2233, 2608, 2740, 2747, 2764
TspRI CASTG 6 cut(s) 188, 1021, 2355, 2378, 2710, 2821
Tth111I GACNNNGTC 1 cut(s) 2254
Van91I CCANNNNNTGG 1 cut(s) 2709
Vha464I CTTAAG 1 cut(s) 584
VneI GTGCAC 1 cut(s) 2346
VpaK11BI GGWCC 2 cut(s) 460, 2830
XagI CCTNNNNNAGG 1 cut(s) 2398
XapI RAATTY 6 cut(s) 369, 453, 808, 1126, 1236, 1436
XceI RCATGY 3 cut(s) 1371, 1733, 1995
XmiI GTMKAC 1 cut(s) 734
XspI CTAG 7 cut(s) 21, 359, 573, 609, 1050, 1146, 1670
ZraI GACGTC 1 cut(s) 193
ZrmI AGTACT 2 cut(s) 2152, 2383
Zsp2I ATGCAT 1 cut(s) 1715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.