Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000477

Physical Location & Seq

Genomic Coordinates Reverse (-)

38130103 .. 38136917

Gene Structure 6815 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1249800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2388 bp

ATGGCTTCAAATGCAGAGGTTAATGGAGACAGTGATACCACCAACAAAGGCAGTATGTGGGTTCTGGATCAGAAGCTTGACCAACCCATGGACGAGGAGGCTGGCAGGCTGAGAACTATGTACAGGGAGAAGAAGTTCTCCGCACTATTGCTTATGCGGCTTGCATTCCAAAGTCTTGGAGTGGTTTTTGGAGATTTGGGTACTTCTCCCTTGTATGTTTTCTACAATACCTTTCCCCATGGGATTAATGATCCGGAGGATGTGGTTGGAGCCCTTTCGTTGATTATATACTCTCTTACTCTTGTACCGCTCCTCAAGTATGTATTTGTTGTTTGTCGGGCAAATGACAGTGGTCAAGGTGGAACATTTGCTCTTTATTCACTTCTCTGTCGGCATGCAAATGTTAAAATTATTCCTAACCAACACCGGACTGATGAGGAGCTAACAACATATAGTCGTTCTACTTTCCATGAACAATCATCTGCTGCAAAAACCAAGAAGTGGTTGGAGGGGCATGCATCAAGGAAGAATGCCCTTCTTCTTCTGGTCCTCATAGGCACTTGTATGGTGATTGGAGATGGAATTCTTACTCCAGTTATATCTGTTTTATCAGCTGCTGGTGGGATCAAAGTCAGAAGTCCTGGCATGAACAATGATTATGTCATACTCGTTGCTGTTGTAATATTAGTAGGTCTGTTTAGCGTGCAGCACTATGGCACAGATAAAGTTGGTTGGCTCTTTGCTCCCATTGTGCTGCTTTGGTTTCTATTAATTGGAGGTATTGGCATTTTCAACATCTGGAAACATGACAGTACTGTTTTGAGAGCTTTTTACCCCGTGTATATATATCGGTATTTTAAAAGGAACGGGAGAGATGGTTGGACTTCTCTTGGGGGTATTATGCTTAGTATAACAGGGACAGAAGCACTCTTTGCCGACCTTGCCTATTTTCCAGTTTCAGCTGTACAGATTGCGTTTACTACAGTCGTGTTTCCTTGCCTTCTTTTGGCCTATTGCGGACAGGCTGCCTTCCTTGTGAAAAATCATGAAGACAAAACTGTGCTTCAAGCATTTTATCTTTCTATTCCAGAACAAATATACTGGCCAGTCTTCATTGTTGCGACTCTTGCTGCCGTAGTTGCTAGTCAAGCAACAATATCAGCAACATTTTCAATAATCAAGCAGGCTCTTGCACTTGGCTGTTTTCCTAGAGTCAAAGTTGTACATACATCAAAGAAGTTCCTCGGCCAAATATATATTCCAGATATTAATTGGATCCTTATGATTCTTTGCATTGCGGTGACTGCTGGGTTCAAAAATCAAAGCCAAATTGGAAATGCTTATGGGACGGCAGTTGTAATAGTCATGCTGGCAACCACATTGCTTATGACTCTAATCATGATATTAGTGTGGCGCTGCCATTGGATTCTTGTCGTTACATTCACCAGCTTGTCGCTGATTGTGGAATGCACTTACTTTTCTGCTGTACTCTGCAAGGTTGATCAAGGTGGATGGGTTCCTCTTGTGATTGCAGCTGCTTTTCTTCTCATCATGTATGTTTGGCATTATGGTACGTTGAAACGTTATGAGTTTGAGATGCACAGTAAAGTTTCAATGGCATGGCTTCTTGGGCTTGGCCCCAGTTTAGGACTGGTTCGGGTCCCTGGGATAGGACTTGTGTACACCGAGCTTGCAAGCGGGGTGCCCCACATCTTTTCCCACTTCATCACCAACCTGCCCGCAATCCACCAGGTTGTTGTCTTTGTCTGTGTTAAGTACCTCCCTGTATACACCGTCCCAGAAGAAGAAAGATTCCTCGTCAAGCGGATTGGCCCCAAGAGTTTCCACATGTTTCGTTGTGTTGCAAGATACGGTTATAAAGACCTACACAAGAAAGATGATGATTTTGAGAAGAAGCTCTTTGAAAACCTTTTCCTGTTTGTCCGGCTCGAGTCCATGATGGAAGGGTGTTCAGACTCAGATGAGTACAGCATCAATGGCCAGCAAACTGAGAGATCAAGGGACGGCCTCTTAACTAATAATGGCAACACAACCACTTCTACCGTAGACCTCAGTATCTCATCAGTGGACTCAATTGTTCCGGCTAAATCTCCACCGCACGCGAACAATACCGTGAGTTCGTACGGGCAGATGAGCAGCCAGAATGAGATTGATGAGCTAGAGTTTCTGAATAATTGTCGAGAGTCCGGGGTGGTGCATATACTTGGGAACACGGTAGTGAGAGCAAGGAGGGATTCCAGGTTCTACAAAAAGATAGCCGTTGATTACATTTATGCATTTCTCAGGAAAATTTGCAGGGAGAATAGTGTGATCTTCAACGTTCCTCACGAGAGTCTATTGAATGTTGGACAGATTTTCTATGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015318 GO:0015672 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

796

Amino Acids

88.76

Weight (kDa)

8.44

Isoelectric Point (pI)

36.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	54 - 541	6.1e-160	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	555 - 792	1e-25	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000477)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G31120 AT2G35060 AT2G35060 AT2G35060 AT2G35060 AT4G19960 AT4G19960 AT4G19960 AT4G19960
fragaria_vesca	FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12500 FvH4_3g12501 FvH4_3g12501 FvH4_3g12502 FvH4_3g12502 FvH4_3g12502
malus_domestica	MD05G1249700.v1.1 MD05G1249800.v1.1 MD10G1230100.v1.1 MD10G1230400.v1.1
prunus_persica	Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114000_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114200_v2.0.a1 Prupe.4G114300_v2.0.a1
pyrus_communis	pycom05g22700 pycom05g22730 pycom10g19380 pycom10g19390 pycom10g19400
rosa_chinensis	RchiOBHm_Chr5g0020451 RchiOBHm_Chr5g0020461 RchiOBHm_Chr5g0020471 RchiOBHm_Chr5g0020491
rosa_laevigata	RLG00000032531 RLG00000032532 RLG00000032533 RLG00000032534
rosa_multiflora	Rmu_sc0000033.1_g000001 Rmu_sc0000033.1_g000004 Rmu_sc0009651.1_g000001 Rmu_sc0009745.1_g000016 Rmu_sc0030432.1_g000001
rosa_roxburghii	Rroxscaffold_1G00057790 Rroxscaffold_1G00057800 Rroxscaffold_1G00057810 Rroxscaffold_1G00057830
rosa_rugosa	Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059500 Rorug05G0059600 Rorug05G0059700 Rorug05G0059800 Rorug05G0059900 Rorug05G0060000 Rorug05G0060100
rosa_samantha	Rh5AG150600 Rh5AG150700 Rh5AG150800 Rh5AG150900 Rh5BG149500 Rh5BG149800 Rh5BG149900 Rh5BG150000 Rh5CG161100 Rh5CG161200 Rh5CG161400 Rh5DG149100 Rh5DG149200 Rh5DG149300 Rh5DG149400
rosa_wichuraiana	Rw5G013300 Rw5G013310 Rw5G013320 Rw5G013330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1878
Acc36I	ACCTGC	1 cut(s)	1743
AccB1I	GGYRCC	1 cut(s)	1702
AccB7I	CCANNNNNTGG	2 cut(s)	88, 501
AccBSI	CCGCTC	1 cut(s)	310
AccI	GTMKAC	2 cut(s)	1788, 2067
AccII	CGCG	1 cut(s)	2123
AccIII	TCCGGA	1 cut(s)	253
AciI	CCGC	9 cut(s)	141, 157, 308, 1017, 1298, 1698, 1740, 1825, 2117
AclI	AACGTT	2 cut(s)	1582, 2340
AclWI	GGATC	5 cut(s)	75, 245, 632, 1270, 1283
AcoI	YGGCCR	3 cut(s)	1103, 1246, 1999
AcsI	RAATTY	2 cut(s)	582, 2310
AfiI	CCNNNNNNNGG	5 cut(s)	88, 501, 1006, 1646, 1670
AflIII	ACRYGT	1 cut(s)	1848
AjnI	CCWGG	4 cut(s)	640, 1663, 1749, 2258
AjuI	GAANNNNNNNTTGG	2 cut(s)	1242, 1274
AleI	CACNNNNGTG	1 cut(s)	2237
AloI	GAACNNNNNNTCC	4 cut(s)	119, 151, 2246, 2278
Alw26I	GTCTC	1 cut(s)	21
AlwI	GGATC	5 cut(s)	75, 245, 632, 1270, 1283
AlwNI	CAGNNNCTG	1 cut(s)	617
Ama87I	CYCGRG	1 cut(s)	1949
Aor13HI	TCCGGA	1 cut(s)	253
AoxI	GGCC	7 cut(s)	1008, 1103, 1246, 1636, 1831, 1999, 2026
ApoI	RAATTY	2 cut(s)	582, 2310
AseI	ATTAAT	3 cut(s)	246, 770, 1269
Asp700I	GAANNNNTTC	1 cut(s)	134
AspLEI	GCGC	1 cut(s)	1416
AspS9I	GGNCC	4 cut(s)	547, 1637, 1660, 1832
AsuC2I	CCSGG	1 cut(s)	2209
AsuHPI	GGTGA	4 cut(s)	580, 1312, 1435, 1720
AvaI	CYCGRG	1 cut(s)	1949
AvaII	GGWCC	2 cut(s)	547, 1660
BaeGI	GKGCMC	1 cut(s)	1707
BaeI	ACNNNNGTAYC	4 cut(s)	288, 321, 2059, 2092
BalI	TGGCCA	2 cut(s)	1105, 2001
BamHI	GGATCC	1 cut(s)	1275
BanI	GGYRCC	1 cut(s)	1702
BanII	GRGCYC	1 cut(s)	274
BauI	CACGAG	1 cut(s)	2348
BbsI	GAAGAC	2 cut(s)	1056, 1102
BccI	CCATC	4 cut(s)	572, 869, 1506, 1954
BceAI	ACGGC	4 cut(s)	1118, 1365, 2041, 2264
BciT130I	CCWGG	4 cut(s)	642, 1665, 1751, 2260
BclI	TGATCA	1 cut(s)	1501
BcnI	CCSGG	1 cut(s)	2209
BcoDI	GTCTC	1 cut(s)	21
BfaI	CTAG	3 cut(s)	1143, 1209, 2180
BfmI	CTRYAG	1 cut(s)	981
BfoI	RGCGCY	1 cut(s)	1417
BfuAI	ACCTGC	1 cut(s)	1743
BmcAI	AGTACT	1 cut(s)	814
Bme1390I	CCNGG	5 cut(s)	642, 1665, 1751, 2209, 2260
Bme18I	GGWCC	2 cut(s)	547, 1660
BmeT110I	CYCGRG	1 cut(s)	1949
BmgT120I	GGNCC	4 cut(s)	547, 1637, 1660, 1832
BmiI	GGNNCC	8 cut(s)	271, 1277, 1518, 1639, 1661, 1662, 1704, 1834
BmrFI	CCNGG	5 cut(s)	642, 1665, 1751, 2209, 2260
BmrI	ACTGGG	1 cut(s)	1635
BmsI	GCATC	3 cut(s)	527, 1587, 2001
BmuI	ACTGGG	1 cut(s)	1635
BoxI	GACNNNNGTC	1 cut(s)	351
BpiI	GAAGAC	2 cut(s)	1056, 1102
BpmI	CTGGAG	1 cut(s)	576
BpuEI	CTTGAG	1 cut(s)	299
BpuMI	CCSGG	1 cut(s)	2209
BsaJI	CCNNGG	6 cut(s)	87, 238, 1243, 1663, 1664, 2208
BsaWI	WCCGGW	2 cut(s)	253, 426
BsaXI	ACNNNNNCTCC	4 cut(s)	119, 149, 862, 892
Bsc4I	CCNNNNNNNGG	5 cut(s)	88, 501, 1006, 1646, 1670
Bse1I	ACTGG	6 cut(s)	593, 953, 1106, 1106, 1641, 1656
Bse3DI	GCAATG	2 cut(s)	1293, 1379
BseAI	TCCGGA	1 cut(s)	253
BseBI	CCWGG	4 cut(s)	642, 1665, 1751, 2260
BseDI	CCNNGG	6 cut(s)	87, 238, 1243, 1663, 1664, 2208
BseGI	GGATG	2 cut(s)	265, 1517
BseLI	CCNNNNNNNGG	5 cut(s)	88, 501, 1006, 1646, 1670
BseMI	GCAATG	2 cut(s)	1293, 1379
BseMII	CTCAG	5 cut(s)	101, 1992, 2001, 2086, 2317
BseNI	ACTGG	6 cut(s)	593, 953, 1106, 1106, 1641, 1656
BseRI	GAGGAG	3 cut(s)	110, 302, 452
BseSI	GKGCMC	1 cut(s)	1707
BseYI	CCCAGC	1 cut(s)	1307
BsgI	GTGCAG	1 cut(s)	725
Bsh1236I	CGCG	1 cut(s)	2123
BshFI	GGCC	7 cut(s)	1010, 1105, 1248, 1638, 1833, 2001, 2028
BshNI	GGYRCC	1 cut(s)	1702
BsiHKCI	CYCGRG	1 cut(s)	1949
BsiSI	CCGG	5 cut(s)	254, 427, 1945, 2102, 2208
BsiWI	CGTACG	1 cut(s)	2142
BslFI	GGGAC	5 cut(s)	931, 1360, 1646, 1781, 2036
BslI	CCNNNNNNNGG	5 cut(s)	88, 501, 1006, 1646, 1670
BsmAI	GTCTC	1 cut(s)	21
BsmFI	GGGAC	5 cut(s)	931, 1360, 1646, 1781, 2036
BsmI	GAATGC	3 cut(s)	164, 535, 1472
BsnI	GGCC	7 cut(s)	1010, 1105, 1248, 1638, 1833, 2001, 2028
BsoBI	CYCGRG	1 cut(s)	1949
Bsp1286I	GDGCHC	2 cut(s)	274, 1707
Bsp13I	TCCGGA	1 cut(s)	253
Bsp1407I	TGTACA	4 cut(s)	120, 964, 1222, 1680
Bsp143I	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
Bsp19I	CCATGG	2 cut(s)	87, 238
BspACI	CCGC	9 cut(s)	141, 157, 308, 1017, 1298, 1698, 1740, 1825, 2117
BspANI	GGCC	7 cut(s)	1010, 1105, 1248, 1638, 1833, 2001, 2028
BspCNI	CTCAG	5 cut(s)	102, 1991, 2002, 2085, 2316
BspEI	TCCGGA	1 cut(s)	253
BspFNI	CGCG	1 cut(s)	2123
BspHI	TCATGA	2 cut(s)	1045, 1398
BspLI	GGNNCC	8 cut(s)	271, 1277, 1518, 1639, 1661, 1662, 1704, 1834
BspMI	ACCTGC	1 cut(s)	1743
BspPI	GGATC	5 cut(s)	75, 245, 632, 1270, 1283
BspT107I	GGYRCC	1 cut(s)	1702
BsrBI	CCGCTC	1 cut(s)	310
BsrDI	GCAATG	2 cut(s)	1293, 1379
BsrGI	TGTACA	4 cut(s)	120, 964, 1222, 1680
BsrI	ACTGG	6 cut(s)	593, 953, 1106, 1106, 1641, 1656
BssECI	CCNNGG	6 cut(s)	87, 238, 1243, 1663, 1664, 2208
BssMI	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
BssNAI	GTATAC	1 cut(s)	1789
BssSI	CACGAG	1 cut(s)	2348
BssT1I	CCWWGG	2 cut(s)	87, 238
Bst1107I	GTATAC	1 cut(s)	1789
Bst2BI	CACGAG	1 cut(s)	2348
Bst2UI	CCWGG	4 cut(s)	642, 1665, 1751, 2260
BstAPI	GCANNNNNTGC	1 cut(s)	932
BstAUI	TGTACA	4 cut(s)	120, 964, 1222, 1680
BstDEI	CTNAG	6 cut(s)	110, 905, 1978, 2010, 2072, 2303
BstDSI	CCRYGG	2 cut(s)	87, 238
BstENI	CCTNNNNNAGG	1 cut(s)	1668
BstF5I	GGATG	2 cut(s)	265, 1517
BstFNI	CGCG	1 cut(s)	2123
BstH2I	RGCGCY	1 cut(s)	1417
BstHHI	GCGC	1 cut(s)	1416
BstKTI	GATC	7 cut(s)	70, 253, 627, 1278, 1504, 2018, 2334
BstMAI	GTCTC	1 cut(s)	21
BstMBI	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
BstNI	CCWGG	4 cut(s)	642, 1665, 1751, 2260
BstNSI	RCATGY	3 cut(s)	398, 518, 1852
BstPAI	GACNNNNGTC	1 cut(s)	351
BstSCI	CCNGG	5 cut(s)	640, 1663, 1749, 2207, 2258
BstSFI	CTRYAG	1 cut(s)	981
BstSLI	GKGCMC	1 cut(s)	1707
BstUI	CGCG	1 cut(s)	2123
BstV2I	GAAGAC	2 cut(s)	1056, 1102
BstX2I	RGATCY	1 cut(s)	1275
BstXI	CCANNNNNNTGG	1 cut(s)	176
BstYI	RGATCY	1 cut(s)	1275
BstZ17I	GTATAC	1 cut(s)	1789
BsuRI	GGCC	7 cut(s)	1010, 1105, 1248, 1638, 1833, 2001, 2028
BtgI	CCRYGG	2 cut(s)	87, 238
BtsCI	GGATG	2 cut(s)	265, 1517
BtsIMutI	CAGTG	3 cut(s)	37, 355, 2091
BveI	ACCTGC	1 cut(s)	1743
CaiI	CAGNNNCTG	1 cut(s)	617
CciI	TCATGA	2 cut(s)	1045, 1398
CfoI	GCGC	1 cut(s)	1416
Cfr13I	GGNCC	4 cut(s)	547, 1637, 1660, 1832
CsiI	ACCWGGT	1 cut(s)	1749
DdeI	CTNAG	6 cut(s)	110, 905, 1978, 2010, 2072, 2303
DpnI	GATC	7 cut(s)	69, 252, 626, 1277, 1503, 2017, 2333
DpnII	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
DraI	TTTAAA	1 cut(s)	859
EaeI	YGGCCR	3 cut(s)	1103, 1246, 1999
Eco130I	CCWWGG	2 cut(s)	87, 238
Eco24I	GRGCYC	1 cut(s)	274
Eco47I	GGWCC	2 cut(s)	547, 1660
Eco88I	CYCGRG	1 cut(s)	1949
EcoNI	CCTNNNNNAGG	1 cut(s)	1668
EcoO109I	RGGNCCY	1 cut(s)	1660
EcoRI	GAATTC	1 cut(s)	582
EcoRII	CCWGG	4 cut(s)	640, 1663, 1749, 2258
EcoT14I	CCWWGG	2 cut(s)	87, 238
EcoT22I	ATGCAT	2 cut(s)	520, 2299
EcoT38I	GRGCYC	1 cut(s)	274
ErhI	CCWWGG	2 cut(s)	87, 238
FalI	AAGNNNNNCTT	2 cut(s)	1904, 1936
FaqI	GGGAC	5 cut(s)	931, 1360, 1646, 1781, 2036
FauI	CCCGC	2 cut(s)	1691, 1747
FbaI	TGATCA	1 cut(s)	1501
FblI	GTMKAC	2 cut(s)	1788, 2067
FokI	GGATG	2 cut(s)	272, 1524
FriOI	GRGCYC	1 cut(s)	274
FspBI	CTAG	3 cut(s)	1143, 1209, 2180
GlaI	GCGC	1 cut(s)	1415
GsaI	CCCAGC	1 cut(s)	1311
GsuI	CTGGAG	1 cut(s)	576
HaeII	RGCGCY	1 cut(s)	1417
HaeIII	GGCC	7 cut(s)	1010, 1105, 1248, 1638, 1833, 2001, 2028
HapII	CCGG	5 cut(s)	254, 427, 1945, 2102, 2208
HhaI	GCGC	1 cut(s)	1416
Hin6I	GCGC	1 cut(s)	1414
HinP1I	GCGC	1 cut(s)	1414
HindIII	AAGCTT	1 cut(s)	74
HpaII	CCGG	5 cut(s)	254, 427, 1945, 2102, 2208
HphI	GGTGA	4 cut(s)	580, 1312, 1435, 1720
Hpy166II	GTNNAC	6 cut(s)	978, 1681, 1683, 1789, 2068, 2089
Hpy188I	TCNGA	5 cut(s)	72, 635, 1975, 1981, 2190
Hpy8I	GTNNAC	6 cut(s)	978, 1681, 1683, 1789, 2068, 2089
HpyAV	CCTTC	4 cut(s)	545, 1010, 1039, 1958
HpyCH4IV	ACGT	3 cut(s)	1574, 1582, 2340
HpyF3I	CTNAG	6 cut(s)	110, 905, 1978, 2010, 2072, 2303
HpySE526I	ACGT	3 cut(s)	1574, 1582, 2340
HspAI	GCGC	1 cut(s)	1414
KflI	GGGWCCC	1 cut(s)	1660
Kpn2I	TCCGGA	1 cut(s)	253
Ksp22I	TGATCA	1 cut(s)	1501
Kzo9I	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
LmnI	GCTCC	4 cut(s)	269, 315, 439, 748
LweI	GCATC	3 cut(s)	527, 1587, 2001
MabI	ACCWGGT	1 cut(s)	1749
MaeI	CTAG	3 cut(s)	1143, 1209, 2180
MaeII	ACGT	3 cut(s)	1574, 1582, 2340
MaeIII	GTNAC	2 cut(s)	1300, 1435
MalI	GATC	7 cut(s)	69, 252, 626, 1277, 1503, 2017, 2333
MbiI	CCGCTC	1 cut(s)	310
MboI	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
MfeI	CAATTG	1 cut(s)	2094
MflI	RGATCY	1 cut(s)	1275
MhlI	GDGCHC	2 cut(s)	274, 1707
MlsI	TGGCCA	2 cut(s)	1105, 2001
MluCI	AATT	8 cut(s)	408, 582, 771, 1270, 1329, 2094, 2194, 2310
MluNI	TGGCCA	2 cut(s)	1105, 2001
MlyI	GAGTC	8 cut(s)	1117, 1221, 1384, 1961, 1970, 2084, 2213, 2362
MmeI	TCCRAC	4 cut(s)	247, 486, 860, 2347
Mox20I	TGGCCA	2 cut(s)	1105, 2001
Mph1103I	ATGCAT	2 cut(s)	520, 2299
MroI	TCCGGA	1 cut(s)	253
MroXI	GAANNNNTTC	1 cut(s)	134
MscI	TGGCCA	2 cut(s)	1105, 2001
MseI	TTAA	8 cut(s)	21, 246, 405, 770, 858, 1269, 1773, 2033
MslI	CAYNNNNRTG	4 cut(s)	399, 563, 1298, 2237
Msp20I	TGGCCA	2 cut(s)	1105, 2001
MspA1I	CMGCKG	3 cut(s)	614, 962, 1535
MspI	CCGG	5 cut(s)	254, 427, 1945, 2102, 2208
MspR9I	CCNGG	5 cut(s)	642, 1665, 1751, 2209, 2260
MunI	CAATTG	1 cut(s)	2094
Mva1269I	GAATGC	3 cut(s)	164, 535, 1472
MvaI	CCWGG	4 cut(s)	642, 1665, 1751, 2260
MvnI	CGCG	1 cut(s)	2123
NciI	CCSGG	1 cut(s)	2209
NcoI	CCATGG	2 cut(s)	87, 238
NdeII	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
NlaIV	GGNNCC	8 cut(s)	271, 1277, 1518, 1639, 1661, 1662, 1704, 1834
NmeAIII	GCCGAG	1 cut(s)	1224
NmuCI	GTSAC	1 cut(s)	1300
NsiI	ATGCAT	2 cut(s)	520, 2299
NspI	RCATGY	3 cut(s)	398, 518, 1852
OliI	CACNNNNGTG	1 cut(s)	2237
PaeI	GCATGC	2 cut(s)	398, 518
PaeR7I	CTCGAG	1 cut(s)	1949
PagI	TCATGA	2 cut(s)	1045, 1398
PasI	CCCWGGG	1 cut(s)	1664
PciI	ACATGT	1 cut(s)	1848
PcsI	WCGNNNNNNNCGW	1 cut(s)	2346
PctI	GAATGC	3 cut(s)	164, 535, 1472
PdmI	GAANNNNTTC	1 cut(s)	134
PfeI	GAWTC	4 cut(s)	1285, 1426, 1812, 2255
Pfl23II	CGTACG	1 cut(s)	2142
PflMI	CCANNNNNTGG	2 cut(s)	88, 501
PleI	GAGTC	8 cut(s)	1117, 1220, 1384, 1960, 1970, 2084, 2212, 2361
PpsI	GAGTC	8 cut(s)	1117, 1220, 1384, 1960, 1970, 2084, 2212, 2361
PpuMI	RGGWCCY	1 cut(s)	1660
PscI	ACATGT	1 cut(s)	1848
PshAI	GACNNNNGTC	1 cut(s)	351
PshBI	ATTAAT	3 cut(s)	246, 770, 1269
PsiI	TTATAA	1 cut(s)	1878
Psp1406I	AACGTT	2 cut(s)	1582, 2340
Psp5II	RGGWCCY	1 cut(s)	1660
Psp6I	CCWGG	4 cut(s)	640, 1663, 1749, 2258
PspFI	CCCAGC	1 cut(s)	1307
PspGI	CCWGG	4 cut(s)	640, 1663, 1749, 2258
PspLI	CGTACG	1 cut(s)	2142
PspN4I	GGNNCC	8 cut(s)	271, 1277, 1518, 1639, 1661, 1662, 1704, 1834
PspPI	GGNCC	4 cut(s)	547, 1637, 1660, 1832
PspPPI	RGGWCCY	1 cut(s)	1660
PspXI	VCTCGAGB	1 cut(s)	1949
PstNI	CAGNNNCTG	1 cut(s)	617
PsuI	RGATCY	1 cut(s)	1275
PvuII	CAGCTG	3 cut(s)	614, 962, 1535
RseI	CAYNNNNRTG	4 cut(s)	399, 563, 1298, 2237
SaqAI	TTAA	8 cut(s)	21, 246, 405, 770, 858, 1269, 1773, 2033
Sau3AI	GATC	7 cut(s)	67, 250, 624, 1275, 1501, 2015, 2331
Sau96I	GGNCC	4 cut(s)	547, 1637, 1660, 1832
ScaI	AGTACT	1 cut(s)	814
SchI	GAGTC	8 cut(s)	1117, 1221, 1384, 1961, 1970, 2084, 2213, 2362
ScrFI	CCNGG	5 cut(s)	642, 1665, 1751, 2209, 2260
SduI	GDGCHC	2 cut(s)	274, 1707
SexAI	ACCWGGT	1 cut(s)	1749
SfaNI	GCATC	3 cut(s)	527, 1587, 2001
SfcI	CTRYAG	1 cut(s)	981
Sfr274I	CTCGAG	1 cut(s)	1949
SinI	GGWCC	2 cut(s)	547, 1660
SlaI	CTCGAG	1 cut(s)	1949
SmiMI	CAYNNNNRTG	4 cut(s)	399, 563, 1298, 2237
SmlI	CTYRAG	2 cut(s)	314, 1949
SmoI	CTYRAG	2 cut(s)	314, 1949
SphI	GCATGC	2 cut(s)	398, 518
Sse9I	AATT	8 cut(s)	408, 582, 771, 1270, 1329, 2094, 2194, 2310
SsiI	CCGC	9 cut(s)	141, 157, 308, 1017, 1298, 1698, 1740, 1825, 2117
SspI	AATATT	1 cut(s)	684
SspMI	CTAG	3 cut(s)	1143, 1209, 2180
StyD4I	CCNGG	5 cut(s)	640, 1663, 1749, 2207, 2258
StyI	CCWWGG	2 cut(s)	87, 238
TaiI	ACGT	3 cut(s)	1577, 1585, 2343
TaqI	TCGA	2 cut(s)	1950, 2200
TasI	AATT	8 cut(s)	408, 582, 771, 1270, 1329, 2094, 2194, 2310
TatI	WGTACW	7 cut(s)	120, 812, 964, 1222, 1486, 1680, 1986
TauI	GCSGC	1 cut(s)	160
TfiI	GAWTC	4 cut(s)	1285, 1426, 1812, 2255
Tru1I	TTAA	8 cut(s)	21, 246, 405, 770, 858, 1269, 1773, 2033
Tru9I	TTAA	8 cut(s)	21, 246, 405, 770, 858, 1269, 1773, 2033
TscAI	CASTG	3 cut(s)	37, 355, 2091
TseFI	GTSAC	1 cut(s)	1300
Tsp45I	GTSAC	1 cut(s)	1300
TspDTI	ATGAA	5 cut(s)	486, 662, 1062, 1102, 1714
TspRI	CASTG	3 cut(s)	37, 355, 2091
Van91I	CCANNNNNTGG	2 cut(s)	88, 501
VpaK11BI	GGWCC	2 cut(s)	547, 1660
VspI	ATTAAT	3 cut(s)	246, 770, 1269
XagI	CCTNNNNNAGG	1 cut(s)	1668
XapI	RAATTY	2 cut(s)	582, 2310
XceI	RCATGY	3 cut(s)	398, 518, 1852
XcmI	CCANNNNNNNNNTGG	3 cut(s)	502, 1269, 1648
XhoI	CTCGAG	1 cut(s)	1949
XmiI	GTMKAC	2 cut(s)	1788, 2067
XmnI	GAANNNNTTC	1 cut(s)	134
XspI	CTAG	3 cut(s)	1143, 1209, 2180
ZrmI	AGTACT	1 cut(s)	814
Zsp2I	ATGCAT	2 cut(s)	520, 2299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.