AT4G35730

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003928
Physical Location & Seq
Forward (+)
16931003 .. 16933499
2497 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35730.1

Sequence Viewer

Length: 1401 bp
ATGACGGCGGCCGAAGTAGCTTCTGCGCAGACCAAGAAGCTCATGAAGTTTAGTCTTTCTCTCTTCCGTCGTGGCTTTAACTCCTCTAAATGCAAAACGGCGGCGAAGATGGCTGTGGCTCGTATAAAGCTTATAAGGAACAAGAGATTAGTTGTGGTGAAGCAAATGAGGCGTGACATCGCTGTTCTTCTTCAATCCGGTCAAGATGCCACTGCTCGAATCAGAGTTGAGCATGTGATAAGGGAACAGAACATTCAGGCAGCAAACGAAATCATTGAGCTTTTCTGTGAACTTATTGTTTCTCGTCTCACTATCATCACTAAGCAAAAGCAATGTCCTGTGGATCTTAAGGAAGGTATTGCTAGTTTGATTTTTGCTGCGCCTAGATGCTCTGAAATTCCTGAGCTTGGAGATCTTAGAGACATCTTTGCGAAGAAATATGGGAAAGATTTTGTATCTGCTGCTACTGATTTGCGTCCTAGCTGCGGTGTTAACCGGATGTTGATTGATAAGCTTTCGGTTAGAAATCCCGGTGGAGAATACAAACTGAAAATCATGAAGGAAATTGCAAAGGAATTCCAGGTTGATTGGGATACTACAGAGACAGAGCAAGAACTTCTCAAACCTCAAGAAGAAAGCATAGACGGGCCACGTAAGTTTGTTAGCGCTTCAAGCTTACCAGTTAATCGTGCAGCTATCAACGAGCCTATTGATCCTACCAAAGCAGTGCCAAGATCGACTAGTAGCATGAGCATCAACACGCATTATCATGACACCGAGTCAGCAGCGGAAGCAGCTACAGAATTAGCCAAACAGGCAGTTGCAGCAGCACAAGTAGCCTCCTTATTAGCTACCAGAAGAGACAGCAGCAACAAGGAGTTCTCTGTATCTTCTGATCATTCAACACACCAGAAGGATTCACAGTACATGGATCATCATCATCATCATCCAGGTTCAAGAAGACAATCACGAGACTCCGAAACAAGTTCCTATTACGCAAAACCTGGTGCAGAAAACAGAGGAATGGGTAGGAGACACAGCTACAACAATCCGGGTATTAATGAATCTGACTACGAGGAAGAGTATACCAATACCGAAGCAGAAGCAAAAGAAACAATGAGGAGGAGACACAGCTACAACCCTCGATCAGTTCCTCCTCCTGCAACTTCTGAGATCAAGTTTGATGAGTCAGACTACTACGAGGAAGAGACAGAGCCAGATGAACCATCACAGGGTCGTGTTTCTTCTCTCCCGCCAAACCGAGCACCGCCTCAGGCTCCTCAGTCCGGTGAGTCCAGACAGGACTCGAGCGGTCACCAGGTTCACCCAAAACTGCCTGACTATGATATTTTGGCTGCACGTTTTGAAGCAATCAGACACAGTAAAGGGCCATTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

466

Amino Acids

52.2

Weight (kDa)

8.13

Isoelectric Point (pI)

61.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 3.9e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
Acc16I TGCGCA 1 cut(s) 27
AccBSI CCGCTC 1 cut(s) 1311
AccI GTMKAC 1 cut(s) 1085
AciI CCGC 7 cut(s) 8, 101, 486, 788, 1253, 1268, 1311
AclWI GGATC 3 cut(s) 351, 707, 939
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 2 cut(s) 396, 575
AfaI GTAC 1 cut(s) 926
AfeI AGCGCT 1 cut(s) 667
AfiI CCNNNNNNNGG 4 cut(s) 407, 485, 1232, 1286
AflII CTTAAG 1 cut(s) 347
AgsI TTSAA 5 cut(s) 194, 672, 903, 957, 1367
AhdI GACNNNNNGTC 1 cut(s) 778
AhlI ACTAGT 1 cut(s) 740
AjnI CCWGG 4 cut(s) 579, 949, 1003, 1317
Alw21I GWGCWC 1 cut(s) 1267
Alw26I GTCTC 8 cut(s) 311, 414, 596, 855, 966, 1027, 1120, 1202
AlwI GGATC 3 cut(s) 351, 707, 939
Ama87I CYCGRG 1 cut(s) 1306
Aor51HI AGCGCT 1 cut(s) 667
AoxI GGCC 3 cut(s) 9, 647, 1388
ApoI RAATTY 2 cut(s) 396, 575
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AseI ATTAAT 1 cut(s) 1059
AspLEI GCGC 3 cut(s) 28, 382, 668
AspS9I GGNCC 2 cut(s) 647, 1388
AsuC2I CCSGG 2 cut(s) 531, 1053
AsuHPI GGTGA 4 cut(s) 169, 1301, 1307, 1316
AvaI CYCGRG 1 cut(s) 1306
AxyI CCTNAGG 1 cut(s) 1272
BauI CACGAG 1 cut(s) 969
BbsI GAAGAC 1 cut(s) 967
Bbv12I GWGCWC 1 cut(s) 1267
BccI CCATC 2 cut(s) 103, 1234
BceAI ACGGC 2 cut(s) 21, 114
BciT130I CCWGG 4 cut(s) 581, 951, 1005, 1319
BciVI GTATCC 1 cut(s) 586
BclI TGATCA 1 cut(s) 895
BcnI CCSGG 2 cut(s) 531, 1053
BcoDI GTCTC 8 cut(s) 311, 414, 596, 855, 966, 1027, 1120, 1202
BcuI ACTAGT 1 cut(s) 740
BfaI CTAG 4 cut(s) 363, 384, 480, 741
BfmI CTRYAG 2 cut(s) 597, 798
BfoI RGCGCY 1 cut(s) 669
BfrI CTTAAG 1 cut(s) 347
BfuI GTATCC 1 cut(s) 586
BglI GCCNNNNNGGC 1 cut(s) 815
BglII AGATCT 1 cut(s) 412
Bme1390I CCNGG 6 cut(s) 531, 581, 951, 1005, 1053, 1319
BmeRI GACNNNNNGTC 1 cut(s) 778
BmeT110I CYCGRG 1 cut(s) 1306
BmgT120I GGNCC 2 cut(s) 647, 1388
BmiI GGNNCC 1 cut(s) 1278
BmrFI CCNGG 6 cut(s) 531, 581, 951, 1005, 1053, 1319
BmsI GCATC 3 cut(s) 196, 377, 762
BpiI GAAGAC 1 cut(s) 967
Bpu10I CCTNAGC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 612
BpuMI CCSGG 2 cut(s) 531, 1053
BsaAI YACGTR 1 cut(s) 653
BsaBI GATNNNNATC 1 cut(s) 936
BsaWI WCCGGW 3 cut(s) 197, 495, 1286
Bsc4I CCNNNNNNNGG 4 cut(s) 407, 485, 1232, 1286
Bse1I ACTGG 1 cut(s) 680
Bse21I CCTNAGG 1 cut(s) 1272
Bse3DI GCAATG 1 cut(s) 338
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 4 cut(s) 581, 951, 1005, 1319
BseGI GGATG 2 cut(s) 504, 946
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 4 cut(s) 407, 485, 1232, 1286
BseMI GCAATG 1 cut(s) 338
BseMII CTCAG 4 cut(s) 393, 1161, 1286, 1295
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 5 cut(s) 73, 1135, 1138, 1146, 1269
BseX3I CGGCCG 1 cut(s) 9
BsgI GTGCAG 3 cut(s) 711, 1029, 1341
Bsh1285I CGRYCG 1 cut(s) 12
BshFI GGCC 3 cut(s) 11, 649, 1390
BsiEI CGRYCG 1 cut(s) 12
BsiHKAI GWGCWC 1 cut(s) 1267
BsiHKCI CYCGRG 1 cut(s) 1306
BsiSI CCGG 5 cut(s) 198, 496, 531, 1052, 1287
BslI CCNNNNNNNGG 4 cut(s) 407, 485, 1232, 1286
BsmAI GTCTC 8 cut(s) 311, 414, 596, 855, 966, 1027, 1120, 1202
BsmBI CGTCTC 1 cut(s) 311
BsnI GGCC 3 cut(s) 11, 649, 1390
BsoBI CYCGRG 1 cut(s) 1306
Bsp1286I GDGCHC 1 cut(s) 1267
Bsp143I GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
BspACI CCGC 7 cut(s) 8, 101, 486, 788, 1253, 1268, 1311
BspANI GGCC 3 cut(s) 11, 649, 1390
BspCNI CTCAG 4 cut(s) 394, 1162, 1285, 1294
BspHI TCATGA 3 cut(s) 42, 555, 769
BspLI GGNNCC 1 cut(s) 1278
BspPI GGATC 3 cut(s) 351, 707, 939
BspTI CTTAAG 1 cut(s) 347
BsrBI CCGCTC 1 cut(s) 1311
BsrDI GCAATG 1 cut(s) 338
BsrI ACTGG 1 cut(s) 680
BssMI GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
BssNAI GTATAC 1 cut(s) 1086
BssSI CACGAG 1 cut(s) 969
Bst1107I GTATAC 1 cut(s) 1086
Bst2BI CACGAG 1 cut(s) 969
Bst2UI CCWGG 4 cut(s) 581, 951, 1005, 1319
Bst4CI ACNGT 2 cut(s) 924, 1382
Bst6I CTCTTC 4 cut(s) 68, 853, 1074, 1200
BstAFI CTTAAG 1 cut(s) 347
BstBAI YACGTR 1 cut(s) 653
BstDEI CTNAG 6 cut(s) 321, 402, 416, 1170, 1272, 1281
BstEII GGTNACC 1 cut(s) 1313
BstF5I GGATG 2 cut(s) 504, 946
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 3 cut(s) 28, 382, 668
BstKTI GATC 9 cut(s) 346, 415, 715, 737, 898, 934, 1148, 1176, 1398
BstMAI GTCTC 8 cut(s) 311, 414, 596, 855, 966, 1027, 1120, 1202
BstMBI GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
BstMCI CGRYCG 1 cut(s) 12
BstMWI GCNNNNNNNGC 9 cut(s) 17, 110, 169, 672, 791, 794, 815, 824, 836
BstNI CCWGG 4 cut(s) 581, 951, 1005, 1319
BstNSI RCATGY 1 cut(s) 236
BstPI GGTNACC 1 cut(s) 1313
BstSCI CCNGG 6 cut(s) 529, 579, 949, 1003, 1051, 1317
BstSFI CTRYAG 2 cut(s) 597, 798
BstV2I GAAGAC 1 cut(s) 967
BstX2I RGATCY 2 cut(s) 343, 412
BstYI RGATCY 2 cut(s) 343, 412
BstZ17I GTATAC 1 cut(s) 1086
BstZI CGGCCG 1 cut(s) 9
Bsu36I CCTNAGG 1 cut(s) 1272
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 3 cut(s) 11, 649, 1390
BtgZI GCGATG 1 cut(s) 163
BtsCI GGATG 2 cut(s) 504, 946
BtsI GCAGTG 2 cut(s) 210, 732
BtsIMutI CAGTG 2 cut(s) 210, 732
CciI TCATGA 3 cut(s) 42, 555, 769
CfoI GCGC 3 cut(s) 28, 382, 668
Cfr13I GGNCC 2 cut(s) 647, 1388
CseI GACGC 1 cut(s) 464
CsiI ACCWGGT 2 cut(s) 1003, 1317
Csp6I GTAC 1 cut(s) 925
CviAII CATG 6 cut(s) 43, 233, 556, 748, 770, 928
CviQI GTAC 1 cut(s) 925
DdeI CTNAG 6 cut(s) 321, 402, 416, 1170, 1272, 1281
DpnI GATC 9 cut(s) 345, 414, 714, 736, 897, 933, 1147, 1175, 1397
DpnII GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
DriI GACNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 1 cut(s) 9
EagI CGGCCG 1 cut(s) 9
Eam1104I CTCTTC 4 cut(s) 68, 853, 1074, 1200
Eam1105I GACNNNNNGTC 1 cut(s) 778
EarI CTCTTC 4 cut(s) 68, 853, 1074, 1200
EclXI CGGCCG 1 cut(s) 9
Eco47III AGCGCT 1 cut(s) 667
Eco52I CGGCCG 1 cut(s) 9
Eco81I CCTNAGG 1 cut(s) 1272
Eco88I CYCGRG 1 cut(s) 1306
Eco91I GGTNACC 1 cut(s) 1313
EcoO65I GGTNACC 1 cut(s) 1313
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 4 cut(s) 579, 949, 1003, 1317
Esp3I CGTCTC 1 cut(s) 311
FaeI CATG 6 cut(s) 46, 236, 559, 751, 773, 931
FatI CATG 6 cut(s) 42, 232, 555, 747, 769, 927
FauI CCCGC 1 cut(s) 1260
FbaI TGATCA 1 cut(s) 895
FblI GTMKAC 1 cut(s) 1085
FokI GGATG 2 cut(s) 511, 933
FspBI CTAG 4 cut(s) 363, 384, 480, 741
FspI TGCGCA 1 cut(s) 27
GlaI GCGC 3 cut(s) 27, 381, 667
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 3 cut(s) 11, 649, 1390
HapII CCGG 5 cut(s) 198, 496, 531, 1052, 1287
HgaI GACGC 1 cut(s) 464
HhaI GCGC 3 cut(s) 28, 382, 668
Hin1II CATG 6 cut(s) 46, 236, 559, 751, 773, 931
Hin6I GCGC 3 cut(s) 26, 380, 666
HinP1I GCGC 3 cut(s) 26, 380, 666
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HindIII AAGCTT 3 cut(s) 128, 512, 673
HinfI GANTC 8 cut(s) 219, 779, 917, 974, 1064, 1187, 1292, 1304
HpaI GTTAAC 1 cut(s) 493
HpaII CCGG 5 cut(s) 198, 496, 531, 1052, 1287
HphI GGTGA 4 cut(s) 169, 1301, 1307, 1316
Hpy166II GTNNAC 4 cut(s) 290, 493, 1086, 1324
Hpy188I TCNGA 9 cut(s) 224, 394, 895, 979, 1069, 1171, 1192, 1376, 1400
Hpy188III TCNNGA 9 cut(s) 43, 203, 401, 556, 629, 770, 957, 969, 1296
Hpy8I GTNNAC 4 cut(s) 290, 493, 1086, 1324
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 3 cut(s) 347, 553, 907
HpyCH4III ACNGT 2 cut(s) 924, 1382
HpyCH4IV ACGT 2 cut(s) 652, 1360
HpyCH4V TGCA 7 cut(s) 93, 569, 692, 824, 1010, 1163, 1358
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 110, 169, 672, 791, 794, 815, 824, 836
HpyF3I CTNAG 6 cut(s) 321, 402, 416, 1170, 1272, 1281
HpySE526I ACGT 2 cut(s) 652, 1360
Hsp92II CATG 6 cut(s) 46, 236, 559, 751, 773, 931
HspAI GCGC 3 cut(s) 26, 380, 666
Ksp22I TGATCA 1 cut(s) 895
KspAI GTTAAC 1 cut(s) 493
Kzo9I GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
LmnI GCTCC 1 cut(s) 1282
LweI GCATC 3 cut(s) 196, 377, 762
MabI ACCWGGT 2 cut(s) 1003, 1317
MaeI CTAG 4 cut(s) 363, 384, 480, 741
MaeII ACGT 2 cut(s) 652, 1360
MaeIII GTNAC 2 cut(s) 173, 1313
MalI GATC 9 cut(s) 345, 414, 714, 736, 897, 933, 1147, 1175, 1397
MbiI CCGCTC 1 cut(s) 1311
MboI GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
MflI RGATCY 2 cut(s) 343, 412
MhlI GDGCHC 1 cut(s) 1267
MluCI AATT 4 cut(s) 396, 564, 575, 803
MlyI GAGTC 5 cut(s) 788, 968, 1196, 1298, 1301
MseI TTAA 5 cut(s) 78, 348, 492, 684, 1059
MslI CAYNNNNRTG 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 788
MspCI CTTAAG 1 cut(s) 347
MspI CCGG 5 cut(s) 198, 496, 531, 1052, 1287
MspR9I CCNGG 6 cut(s) 531, 581, 951, 1005, 1053, 1319
MvaI CCWGG 4 cut(s) 581, 951, 1005, 1319
MwoI GCNNNNNNNGC 9 cut(s) 17, 110, 169, 672, 791, 794, 815, 824, 836
NciI CCSGG 2 cut(s) 531, 1053
NdeII GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
NlaIII CATG 6 cut(s) 46, 236, 559, 751, 773, 931
NlaIV GGNNCC 1 cut(s) 1278
NmuCI GTSAC 2 cut(s) 173, 1313
NsbI TGCGCA 1 cut(s) 27
NspI RCATGY 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 1306
PagI TCATGA 3 cut(s) 42, 555, 769
PfeI GAWTC 3 cut(s) 219, 917, 1064
PleI GAGTC 5 cut(s) 787, 968, 1195, 1298, 1300
PpsI GAGTC 5 cut(s) 787, 968, 1195, 1298, 1300
Ppu21I YACGTR 1 cut(s) 653
PshBI ATTAAT 1 cut(s) 1059
PsiI TTATAA 1 cut(s) 134
Psp6I CCWGG 4 cut(s) 579, 949, 1003, 1317
PspEI GGTNACC 1 cut(s) 1313
PspGI CCWGG 4 cut(s) 579, 949, 1003, 1317
PspN4I GGNNCC 1 cut(s) 1278
PspPI GGNCC 2 cut(s) 647, 1388
PspXI VCTCGAGB 1 cut(s) 1306
PsuI RGATCY 2 cut(s) 343, 412
RsaI GTAC 1 cut(s) 926
RsaNI GTAC 1 cut(s) 925
RseI CAYNNNNRTG 1 cut(s) 768
SaqAI TTAA 5 cut(s) 78, 348, 492, 684, 1059
Sau3AI GATC 9 cut(s) 343, 412, 712, 734, 895, 931, 1145, 1173, 1395
Sau96I GGNCC 2 cut(s) 647, 1388
SchI GAGTC 5 cut(s) 788, 968, 1196, 1298, 1301
ScrFI CCNGG 6 cut(s) 531, 581, 951, 1005, 1053, 1319
SduI GDGCHC 1 cut(s) 1267
SexAI ACCWGGT 2 cut(s) 1003, 1317
SfaNI GCATC 3 cut(s) 196, 377, 762
SfcI CTRYAG 2 cut(s) 597, 798
Sfr274I CTCGAG 1 cut(s) 1306
SlaI CTCGAG 1 cut(s) 1306
SmiMI CAYNNNNRTG 1 cut(s) 768
SmlI CTYRAG 3 cut(s) 347, 627, 1306
SmoI CTYRAG 3 cut(s) 347, 627, 1306
SpeI ACTAGT 1 cut(s) 740
Sse9I AATT 4 cut(s) 396, 564, 575, 803
SsiI CCGC 7 cut(s) 8, 101, 486, 788, 1253, 1268, 1311
SspMI CTAG 4 cut(s) 363, 384, 480, 741
StyD4I CCNGG 6 cut(s) 529, 579, 949, 1003, 1051, 1317
TaaI ACNGT 2 cut(s) 924, 1382
TaiI ACGT 2 cut(s) 655, 1363
TaqI TCGA 4 cut(s) 217, 737, 1144, 1307
TasI AATT 4 cut(s) 396, 564, 575, 803
TatI WGTACW 1 cut(s) 924
TauI GCSGC 2 cut(s) 11, 104
TfiI GAWTC 3 cut(s) 219, 917, 1064
Tru1I TTAA 5 cut(s) 78, 348, 492, 684, 1059
Tru9I TTAA 5 cut(s) 78, 348, 492, 684, 1059
TscAI CASTG 2 cut(s) 217, 732
TseFI GTSAC 2 cut(s) 173, 1313
Tsp45I GTSAC 2 cut(s) 173, 1313
TspDTI ATGAA 4 cut(s) 59, 572, 1077, 1236
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 2 cut(s) 217, 732
Vha464I CTTAAG 1 cut(s) 347
VspI ATTAAT 1 cut(s) 1059
XapI RAATTY 2 cut(s) 396, 575
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 1306
XmiI GTMKAC 1 cut(s) 1085
XspI CTAG 4 cut(s) 363, 384, 480, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.