Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003928

Physical Location & Seq

Genomic Coordinates Reverse (-)

19728713 .. 19731775

Gene Structure 3063 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1122700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3063 bp

ATGGTATCACCCATATCGATTCCTGGATTCCCTACTGTGCACACCCGCCGATCCGCTGCTACTTGCGACTCTGCCTTGGCGTCCCGTCTTGATGCCGTGGAGGCTTCCTTGGCGGCTCTCATCGCCTCTTTACCTTCCCTCATCGCCACTGAGTACGCCAAGGCCTTCGACAATGTCTTTGATGCCTCTTTTGAAAGATTTCGTTGGGGAATGCTTTCTCGAGGTGCTGCGGTAGGCATCTCTGCCCCTCTTGCCATTGATCATCATGTTTCTCCTGCGATTGAGCCTATCCAGCTACCTCGGAGCCCAATTTGTGCTGAAGGAGCCCAATCGCAGCTTATTCCGTCGGCAATCCAAGCTCCACCGCTGCTCTCATCTTCACCGTTGTTGCGAGCACTGTCAACTTCGGCCGTCAAGCCACACTCGAAGCACCCCGCAGCAGCTATAGTTTCATTTACGAAATCGAAGACGGGGATTCATACTTCGATGATGACCTCTGCTCCGTCACTACTCCATGAGCACCCGTTCTCTGATACTACGAAAACTGGACCTGAGGTAGTTCTTAATTTGAGTATCATTTTAGCAACTTCGGAGGCTACATTCCCTTCTCTTTTGACTGATTACTACGGTGCATACCTGAAAGATAAATTTGGATGGGTTAATTGGTATCTTGTGGGTAAATTTGTGAAGAAAAATTTATATGCATTTGATCTGTTTGATGAAATGTCTAAGAGACAGAATGACAATATGTTCATTCAGCCACTGGTTCACAGTATTGAAGTTCTCCATCTCGACTATCTTGCCATCCAGCTCCATTATTTTGACTTAAATTGCTACCTTGGTTTTGAATATGGAAGTACAATTGCTTTAGGTAGAGTTTTTGTGAGGGGATGGGCAGTGGCTGCCAATGAGTTCATTGAGCTCTTATGTGAATTAGTAGTATCTAGACTTTCTATCATCGCAGAGCAAATGGAGTGTCTGCCTAGTCTCAAAGAAGGAATTGCTAGTTTAATATTTGCCTATCCCAGGTGTTCTGAGATTTCTGAACTGATCGCACTAACAAATGTGTTTGAGAAAAAGCATGGTAAAGATTTTGTATCTGAGGCTGTTGATGTACGTCCCAACTGTGGTGTGAATCGCATGCTGATTGACAAGCTCTCTGTTAGAATCCCTATGGGCGAAGTAAAGTTGAAACTCCTGAAGGAGATAGCCAAGGAATACCACGTTGAGTGGGATACATCGAAATCTGAACAGGAGCTGTTCAAGCCTCCAGAAGAGCTTATACAAGGATGCAATTTTGGTAGTACTACCAGCATGCCTGTGCACAGTGTGCAGCCTCAATCTGTTAAGACTAATAAACCAAACATTAGTTCTTCACGCAGGACAATTGGTGGTGAAAAAGGACATATGCAGTTTGTAGACTCAGCATCAGCTGCTAAAGCAGCTGTAACATCTGCCAATGATGCAATGGCTGCTACACAAGTTGCTGCATATTGGACCAACAGAGACTGCAATCAGGACATTGATATTGTATCTCTCAAAGAAAGAAAAGAAGCCTCCCCTTTGAGTTTGACAGTTTCTTTACGATCAAAGCATGTGGGCAGATTTCAATCTCTTGATCAGTTTCTGTCTTGTGAATATCATATATCTCTTGCTGACAATTTAGAAAGTGAAGTCAAGTCGAGGGATGAAGACTATTCTCAACTCTTTTCTGAAGTGCATAAATTTGATGATGGGATTTCCGAATTCAACAATATGGTGTCTGCAAATCAAGCGATGGTTACACAGTCTGATGTACTTAAGCTTAAGTTTGCTGAAGGGTCCACAATGAGCAAGCTTTGGCTTTTGAATTTGGGTGGCAGTGAGAGACAAGAAAAAACTGAAGTTCAAGGGAAAAGTCTTACGGAATCTCAAAACATCAATAAATCTCTTTCAGCTCTTGGGTATATGATCTCAGCTTTGTCAAGAATTAAGGCTTTGGACCGGTTGGGACATGTGGATGAAGAGAATGCCAAGGCCGTATCCCTGCTTGAGAATGTACTGGATCTTAGTGATTCTATTGACGACGAAGCAATGGTTGCTGGAAATATATTTGGTCTTGTTTATGCACAGCTTGCAGAAGTTTTCTCAAAGTATAGTATGTCCTTTTTAGTTAGTTGTCAAAACCTTGTCCCTCGACCTTGGGTTATCAATGACTTTGACGCAGCAATGGTTGTTTGTGTAATATCTGAGTGCGAGCATTCGGGTGCATTAGACCATAAAAAATGTGTCCATACATACTGCAAAAGAGGCGGCTTTTACACCAACATATCGGTGAAGAATGCCTTGATTGGCATGATAATTGGTCTTGCTATGAAAGGGAAATCTGTTGGAGCACTGGAAATGTTCTCACAAATGGAAGCGTCGAGTGATGTGAAGCCGACATTCCGCGGCGGGTTGTCTGCATGCAGTCATGGTGGGTTTGTGGATAAAGGTTTTCACCATTTCGGAGCCATTACTGAAATTTATAAGCTCAGTCCTCGAATCGAACAATACAGATGCATGGGGGATCTTCTTGGTCGTGCTAATCTGTTAAATGAGGTTGAAATATTCATCAGAGATACACCTATTGAACCTGATGTGATTATATGGAAATCTCTGCTTTTTGCGTATAGGACTTATGGGAATATAGTTTTGGCGGAGTTGGTTGCCAAAAGGAGTGAGGTGTTTGAATCCAAAGGGGCTTTTATCAAATTGCTACAGGACAAAGACCTATCTGTAAGATTGGCAGCTTGTAGGTCTCTTTGTTTGCCCAGTGAAGATACTAGTTTTTCAGAAAGAGAATTCATCAATCTTCTCCCTATCTGTTGGGATTTATGCCTTAAGTTGATTAAGGAAGTTCAGGAGTTTGACTCGAAGAAGGCTTGGGAAGAATCTGCCGATGAGTGCCTTCTTTGGATTCAACTTGTTGGTGCTTTGAGGACAGCAACTAATTGGGAGGGCTTCCACAATTCCATTTTAAGCCTTGAGGACAAGGCTAATTTTCAAGGGGGGAATATTGTTATGAACCCAAAATGTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004838 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006520 GO:0006570 GO:0006572 GO:0006725 GO:0006766 GO:0006775 GO:0006807 GO:0008150 GO:0008152 GO:0008483 GO:0009056 GO:0009058 GO:0009063 GO:0009072 GO:0009074 GO:0009110 GO:0009987 GO:0010189 GO:0016054 GO:0016740 GO:0016769 GO:0018130 GO:0019439 GO:0019752 GO:0042360 GO:0042362 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044281 GO:0044282 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046395 GO:0046483 GO:0070547 GO:0071704 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901605 GO:1901606 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00130 ko00270 ko00350 ko00360 ko00400 ko00401 ko00950 ko00960 ko01100 ko01110 ko01130 ko01230 map00130 map00270 map00350 map00360 map00400 map00401 map00950 map00960 map01100 map01110 map01130 map01230

Pfam Domains

Protein Families

Aminotran_1_2

Protein Analysis

1021

Amino Acids

112.77

Weight (kDa)

5.78

Isoelectric Point (pI)

42.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ist1	PF03398	300 - 410	3.1e-30	Regulator of Vps4 activity in the MVB pathway
Kinesin	PF00225	607 - 678	2.4e-11	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003928)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35730
fragaria_vesca	FvH4_1g10740 FvH4_5g10670
malus_domestica	MD02G1121300.v1.1 MD04G1038700.v1.1 MD14G1122700.v1.1 MD15G1235100.v1.1 MD15G1309100.v1.1
prunus_persica	Prupe.7G178000_v2.0.a1
pyrus_communis	pycom02g09440 pycom03g12370 pycom03g12380 pycom04g03150 pycom06g01650 pycom08g13410 pycom08g13420 pycom10g11000 pycom15g20930
rosa_chinensis	RchiOBHm_Chr2g0097911
rosa_laevigata	RLG00000016744
rosa_multiflora	Rmu_co8027370.1_g000001 Rmu_co8137744.1_g000001 Rmu_co8232697.1_g000001 Rmu_sc0009572.1_g000007 Rmu_sc0018984.1_g000001 Rmu_sc0034225.1_g000001
rosa_roxburghii	Rroxscaffold_2G00144460
rosa_rugosa	Rorug02G0069100
rosa_samantha	Rh2AG116000 Rh2BG118800 Rh2CG120200 Rh2CG120400 Rh2DG120100
rosa_wichuraiana	Rw2G009070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2508
AccI	GTMKAC	1 cut(s)	1419
AccII	CGCG	1 cut(s)	2430
AclWI	GGATC	3 cut(s)	45, 2052, 2556
AcoI	YGGCCR	1 cut(s)	408
AcsI	RAATTY	8 cut(s)	647, 680, 694, 1724, 1745, 1849, 2502, 2822
AcuI	CTGAAG	5 cut(s)	339, 1220, 1734, 1836, 1902
AcyI	GRCGYC	1 cut(s)	80
AdeI	CACNNNGTG	1 cut(s)	1330
AfaI	GTAC	6 cut(s)	155, 859, 1116, 1306, 1797, 2040
AfiI	CCNNNNNNNGG	2 cut(s)	1026, 1127
AflII	CTTAAG	3 cut(s)	1799, 1805, 2861
AflIII	ACRYGT	1 cut(s)	1993
AgeI	ACCGGT	1 cut(s)	1983
AhlI	ACTAGT	1 cut(s)	2804
AjnI	CCWGG	2 cut(s)	22, 1025
AjuI	GAANNNNNNNTTGG	4 cut(s)	1354, 1386, 2657, 2689
Alw21I	GWGCWC	6 cut(s)	42, 397, 522, 924, 1326, 2377
Alw26I	GTCTC	5 cut(s)	727, 992, 1500, 1861, 2784
Alw44I	GTGCAC	2 cut(s)	38, 1322
AlwI	GGATC	3 cut(s)	45, 2052, 2556
AlwNI	CAGNNNCTG	5 cut(s)	763, 902, 1258, 1509, 1627
Ama87I	CYCGRG	1 cut(s)	219
AoxI	GGCC	3 cut(s)	162, 408, 2016
ApaLI	GTGCAC	2 cut(s)	38, 1322
ApoI	RAATTY	8 cut(s)	647, 680, 694, 1724, 1745, 1849, 2502, 2822
ArsI	GACNNNNNNTTYG	4 cut(s)	161, 193, 961, 993
AsiGI	ACCGGT	1 cut(s)	1983
Asp700I	GAANNNNTTC	2 cut(s)	198, 214
AspS9I	GGNCC	4 cut(s)	548, 1497, 1821, 1981
AsuHPI	GGTGA	4 cut(s)	372, 1406, 2326, 2471
AvaI	CYCGRG	1 cut(s)	219
AvaII	GGWCC	4 cut(s)	548, 1497, 1821, 1981
AxyI	CCTNAGG	1 cut(s)	552
BaeGI	GKGCMC	2 cut(s)	42, 1326
BanII	GRGCYC	3 cut(s)	308, 328, 924
BarI	GAAGNNNNNNTAC	2 cut(s)	1266, 1298
BbsI	GAAGAC	2 cut(s)	473, 1698
Bbv12I	GWGCWC	6 cut(s)	42, 397, 522, 924, 1326, 2377
BccI	CCATC	6 cut(s)	648, 795, 812, 885, 1727, 1771
BceAI	ACGGC	3 cut(s)	80, 395, 2003
BcgI	CGANNNNNNTGC	2 cut(s)	782, 816
BciT130I	CCWGG	2 cut(s)	24, 1027
BciVI	GTATCC	2 cut(s)	1228, 2032
BclI	TGATCA	2 cut(s)	259, 1618
BcoDI	GTCTC	5 cut(s)	727, 992, 1500, 1861, 2784
BcuI	ACTAGT	1 cut(s)	2804
BfaI	CTAG	4 cut(s)	945, 984, 1005, 2805
BfmI	CTRYAG	2 cut(s)	444, 2738
BfrI	CTTAAG	3 cut(s)	1799, 1805, 2861
BfuI	GTATCC	2 cut(s)	1228, 2032
BglI	GCCNNNNNGGC	1 cut(s)	101
BmcAI	AGTACT	1 cut(s)	1306
Bme1390I	CCNGG	2 cut(s)	24, 1027
Bme18I	GGWCC	4 cut(s)	548, 1497, 1821, 1981
BmeT110I	CYCGRG	1 cut(s)	219
BmgT120I	GGNCC	4 cut(s)	548, 1497, 1821, 1981
BmiI	GGNNCC	4 cut(s)	305, 325, 1822, 2491
BmrFI	CCNGG	2 cut(s)	24, 1027
BmrI	ACTGGG	1 cut(s)	2787
BmsI	GCATC	7 cut(s)	82, 172, 246, 1280, 1436, 1453, 2528
BmuI	ACTGGG	1 cut(s)	2787
BpiI	GAAGAC	2 cut(s)	473, 1698
BplI	GAGNNNNNCTC	2 cut(s)	2876, 2908
BpmI	CTGGAG	1 cut(s)	1254
BpuEI	CTTGAG	2 cut(s)	2051, 3026
Bsa29I	ATCGAT	1 cut(s)	17
BsaBI	GATNNNNATC	1 cut(s)	1609
BsaHI	GRCGYC	1 cut(s)	80
BsaI	GGTCTC	1 cut(s)	2784
BsaWI	WCCGGW	1 cut(s)	1983
BsaXI	ACNNNNNCTCC	2 cut(s)	484, 514
Bsc4I	CCNNNNNNNGG	2 cut(s)	1026, 1127
Bse118I	RCCGGY	1 cut(s)	1983
Bse1I	ACTGG	5 cut(s)	550, 768, 2046, 2382, 2793
Bse21I	CCTNAGG	1 cut(s)	552
Bse3DI	GCAATG	3 cut(s)	1473, 2079, 2214
Bse8I	GATNNNNATC	1 cut(s)	1609
BseBI	CCWGG	2 cut(s)	24, 1027
BseCI	ATCGAT	1 cut(s)	17
BseGI	GGATG	6 cut(s)	659, 804, 896, 1295, 1693, 2005
BseJI	GATNNNNATC	1 cut(s)	1609
BseLI	CCNNNNNNNGG	2 cut(s)	1026, 1127
BseMI	GCAATG	3 cut(s)	1473, 2079, 2214
BseMII	CTCAG	8 cut(s)	141, 543, 1026, 1092, 1437, 1968, 2220, 2527
BseNI	ACTGG	5 cut(s)	550, 768, 2046, 2382, 2793
BseSI	GKGCMC	2 cut(s)	42, 1326
BseX3I	CGGCCG	1 cut(s)	408
BsgI	GTGCAG	1 cut(s)	1352
Bsh1236I	CGCG	1 cut(s)	2430
Bsh1285I	CGRYCG	1 cut(s)	411
BshFI	GGCC	3 cut(s)	164, 410, 2018
BshTI	ACCGGT	1 cut(s)	1983
BshVI	ATCGAT	1 cut(s)	17
BsiEI	CGRYCG	1 cut(s)	411
BsiHKAI	GWGCWC	6 cut(s)	42, 397, 522, 924, 1326, 2377
BsiHKCI	CYCGRG	1 cut(s)	219
BsiSI	CCGG	1 cut(s)	1984
BslFI	GGGAC	4 cut(s)	67, 1104, 2004, 2156
BslI	CCNNNNNNNGG	2 cut(s)	1026, 1127
BsmAI	GTCTC	5 cut(s)	727, 992, 1500, 1861, 2784
BsmFI	GGGAC	4 cut(s)	67, 1104, 2004, 2156
BsmI	GAATGC	4 cut(s)	216, 2014, 2239, 2326
BsnI	GGCC	3 cut(s)	164, 410, 2018
Bso31I	GGTCTC	1 cut(s)	2784
BsoBI	CYCGRG	1 cut(s)	219
Bsp1286I	GDGCHC	8 cut(s)	42, 308, 328, 397, 522, 924, 1326, 2377
Bsp143I	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
BspANI	GGCC	3 cut(s)	164, 410, 2018
BspCNI	CTCAG	8 cut(s)	142, 544, 1027, 1093, 1436, 1967, 2221, 2526
BspDI	ATCGAT	1 cut(s)	17
BspFNI	CGCG	1 cut(s)	2430
BspLI	GGNNCC	4 cut(s)	305, 325, 1822, 2491
BspPI	GGATC	3 cut(s)	45, 2052, 2556
BspQI	GCTCTTC	1 cut(s)	1269
BspTI	CTTAAG	3 cut(s)	1799, 1805, 2861
BspTNI	GGTCTC	1 cut(s)	2784
BsrDI	GCAATG	3 cut(s)	1473, 2079, 2214
BsrFI	RCCGGY	1 cut(s)	1983
BsrI	ACTGG	5 cut(s)	550, 768, 2046, 2382, 2793
BssAI	RCCGGY	1 cut(s)	1983
BssMI	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
BssNI	GRCGYC	1 cut(s)	80
BssT1I	CCWWGG	7 cut(s)	75, 108, 159, 838, 1212, 2013, 2180
Bst2UI	CCWGG	2 cut(s)	24, 1027
Bst4CI	ACNGT	9 cut(s)	37, 384, 399, 629, 773, 1127, 1328, 1576, 1788
Bst6I	CTCTTC	2 cut(s)	1269, 1998
BstACI	GRCGYC	1 cut(s)	80
BstAFI	CTTAAG	3 cut(s)	1799, 1805, 2861
BstAPI	GCANNNNNTGC	6 cut(s)	902, 1330, 1433, 1472, 2078, 2114
BstC8I	GCNNGC	7 cut(s)	393, 1142, 1316, 1835, 2115, 2237, 2446
BstDSI	CCRYGG	2 cut(s)	96, 2428
BstENI	CCTNNNNNAGG	1 cut(s)	1024
BstF5I	GGATG	6 cut(s)	659, 804, 896, 1295, 1693, 2005
BstFNI	CGCG	1 cut(s)	2430
BstKTI	GATC	9 cut(s)	53, 262, 712, 1053, 1589, 1621, 1953, 2047, 2551
BstMAI	GTCTC	5 cut(s)	727, 992, 1500, 1861, 2784
BstMBI	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
BstMCI	CGRYCG	1 cut(s)	411
BstNI	CCWGG	2 cut(s)	24, 1027
BstNSI	RCATGY	5 cut(s)	1144, 1318, 1598, 1997, 2448
BstSCI	CCNGG	2 cut(s)	22, 1025
BstSFI	CTRYAG	2 cut(s)	444, 2738
BstSLI	GKGCMC	2 cut(s)	42, 1326
BstUI	CGCG	1 cut(s)	2430
BstV2I	GAAGAC	2 cut(s)	473, 1698
BstX2I	RGATCY	2 cut(s)	2044, 2548
BstYI	RGATCY	2 cut(s)	2044, 2548
BstZI	CGGCCG	1 cut(s)	408
Bsu15I	ATCGAT	1 cut(s)	17
Bsu36I	CCTNAGG	1 cut(s)	552
BsuI	GTATCC	2 cut(s)	1228, 2032
BsuRI	GGCC	3 cut(s)	164, 410, 2018
BsuTUI	ATCGAT	1 cut(s)	17
BtgI	CCRYGG	2 cut(s)	96, 2428
BtgZI	GCGATG	4 cut(s)	106, 127, 943, 1790
BtsCI	GGATG	6 cut(s)	659, 804, 896, 1295, 1693, 2005
BtsI	GCAGTG	2 cut(s)	903, 1867
BtsIMutI	CAGTG	8 cut(s)	147, 395, 761, 903, 1333, 1867, 2375, 2800
Cac8I	GCNNGC	7 cut(s)	393, 1142, 1316, 1835, 2115, 2237, 2446
CaiI	CAGNNNCTG	5 cut(s)	763, 902, 1258, 1509, 1627
Cfr10I	RCCGGY	1 cut(s)	1983
Cfr13I	GGNCC	4 cut(s)	548, 1497, 1821, 1981
Cfr42I	CCGCGG	1 cut(s)	2431
ClaI	ATCGAT	1 cut(s)	17
CseI	GACGC	3 cut(s)	69, 2210, 2391
Csp6I	GTAC	6 cut(s)	154, 858, 1115, 1305, 1796, 2039
CspAI	ACCGGT	1 cut(s)	1983
CspCI	CAANNNNNGTGG	2 cut(s)	1578, 1613
CviQI	GTAC	6 cut(s)	154, 858, 1115, 1305, 1796, 2039
DpnI	GATC	9 cut(s)	52, 261, 711, 1052, 1588, 1620, 1952, 2046, 2550
DpnII	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
DraIII	CACNNNGTG	1 cut(s)	1330
EaeI	YGGCCR	1 cut(s)	408
EagI	CGGCCG	1 cut(s)	408
Eam1104I	CTCTTC	2 cut(s)	1269, 1998
EarI	CTCTTC	2 cut(s)	1269, 1998
EciI	GGCGGA	1 cut(s)	2693
Ecl136II	GAGCTC	1 cut(s)	922
EclXI	CGGCCG	1 cut(s)	408
Eco130I	CCWWGG	7 cut(s)	75, 108, 159, 838, 1212, 2013, 2180
Eco147I	AGGCCT	1 cut(s)	164
Eco24I	GRGCYC	3 cut(s)	308, 328, 924
Eco31I	GGTCTC	1 cut(s)	2784
Eco47I	GGWCC	4 cut(s)	548, 1497, 1821, 1981
Eco52I	CGGCCG	1 cut(s)	408
Eco53kI	GAGCTC	1 cut(s)	922
Eco57I	CTGAAG	5 cut(s)	339, 1220, 1734, 1836, 1902
Eco81I	CCTNAGG	1 cut(s)	552
Eco88I	CYCGRG	1 cut(s)	219
EcoICRI	GAGCTC	1 cut(s)	922
EcoNI	CCTNNNNNAGG	1 cut(s)	1024
EcoRI	GAATTC	2 cut(s)	1745, 2822
EcoRII	CCWGG	2 cut(s)	22, 1025
EcoT14I	CCWWGG	7 cut(s)	75, 108, 159, 838, 1212, 2013, 2180
EcoT22I	ATGCAT	2 cut(s)	706, 2543
EcoT38I	GRGCYC	3 cut(s)	308, 328, 924
ErhI	CCWWGG	7 cut(s)	75, 108, 159, 838, 1212, 2013, 2180
FalI	AAGNNNNNCTT	2 cut(s)	2309, 2341
FaqI	GGGAC	4 cut(s)	67, 1104, 2004, 2156
FauI	CCCGC	3 cut(s)	53, 442, 2426
FauNDI	CATATG	1 cut(s)	1407
FbaI	TGATCA	2 cut(s)	259, 1618
FblI	GTMKAC	1 cut(s)	1419
FokI	GGATG	6 cut(s)	666, 791, 903, 1302, 1700, 2012
FriOI	GRGCYC	3 cut(s)	308, 328, 924
FspBI	CTAG	4 cut(s)	945, 984, 1005, 2805
GsuI	CTGGAG	1 cut(s)	1254
HaeIII	GGCC	3 cut(s)	164, 410, 2018
HapII	CCGG	1 cut(s)	1984
HgaI	GACGC	3 cut(s)	69, 2210, 2391
Hin1I	GRCGYC	1 cut(s)	80
HincII	GTYRAC	1 cut(s)	402
HindII	GTYRAC	1 cut(s)	402
HindIII	AAGCTT	2 cut(s)	1802, 1835
HpaII	CCGG	1 cut(s)	1984
HphI	GGTGA	4 cut(s)	372, 1406, 2326, 2471
Hpy166II	GTNNAC	6 cut(s)	40, 402, 769, 1324, 1420, 1824
Hpy8I	GTNNAC	6 cut(s)	40, 402, 769, 1324, 1420, 1824
Hpy99I	CGWCG	3 cut(s)	349, 2069, 2407
HpyAV	CCTTC	9 cut(s)	144, 175, 314, 615, 989, 1195, 1811, 2893, 2939
HpyCH4III	ACNGT	9 cut(s)	37, 384, 399, 629, 773, 1127, 1328, 1576, 1788
HpyCH4IV	ACGT	2 cut(s)	1117, 1224
HpySE526I	ACGT	2 cut(s)	1117, 1224
Hsp92I	GRCGYC	1 cut(s)	80
Ksp22I	TGATCA	2 cut(s)	259, 1618
KspI	CCGCGG	1 cut(s)	2431
Kzo9I	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
LguI	GCTCTTC	1 cut(s)	1269
LmnI	GCTCC	8 cut(s)	303, 323, 364, 505, 816, 1255, 2372, 2489
LweI	GCATC	7 cut(s)	82, 172, 246, 1280, 1436, 1453, 2528
MaeI	CTAG	4 cut(s)	945, 984, 1005, 2805
MaeII	ACGT	2 cut(s)	1117, 1224
MaeIII	GTNAC	3 cut(s)	504, 1447, 1780
MalI	GATC	9 cut(s)	52, 261, 711, 1052, 1588, 1620, 1952, 2046, 2550
MboI	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
MfeI	CAATTG	2 cut(s)	861, 1386
MflI	RGATCY	2 cut(s)	2044, 2548
MhlI	GDGCHC	8 cut(s)	42, 308, 328, 397, 522, 924, 1326, 2377
MlyI	GAGTC	3 cut(s)	62, 1415, 2885
MmeI	TCCRAC	1 cut(s)	2350
Mph1103I	ATGCAT	2 cut(s)	706, 2543
MroXI	GAANNNNTTC	2 cut(s)	198, 214
MslI	CAYNNNNRTG	4 cut(s)	1319, 1998, 2244, 2312
MspA1I	CMGCKG	5 cut(s)	56, 367, 1433, 1445, 2430
MspCI	CTTAAG	3 cut(s)	1799, 1805, 2861
MspI	CCGG	1 cut(s)	1984
MspR9I	CCNGG	2 cut(s)	24, 1027
MunI	CAATTG	2 cut(s)	861, 1386
Mva1269I	GAATGC	4 cut(s)	216, 2014, 2239, 2326
MvaI	CCWGG	2 cut(s)	24, 1027
MvnI	CGCG	1 cut(s)	2430
NdeI	CATATG	1 cut(s)	1407
NdeII	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
NlaIV	GGNNCC	4 cut(s)	305, 325, 1822, 2491
NmuCI	GTSAC	1 cut(s)	504
NsiI	ATGCAT	2 cut(s)	706, 2543
NspI	RCATGY	5 cut(s)	1144, 1318, 1598, 1997, 2448
PaeI	GCATGC	3 cut(s)	1144, 1318, 2448
PaeR7I	CTCGAG	1 cut(s)	219
PceI	AGGCCT	1 cut(s)	164
PciI	ACATGT	1 cut(s)	1993
PciSI	GCTCTTC	1 cut(s)	1269
PctI	GAATGC	4 cut(s)	216, 2014, 2239, 2326
PdmI	GAANNNNTTC	2 cut(s)	198, 214
PflFI	GACNNNGTC	1 cut(s)	173
PfoI	TCCNGGA	1 cut(s)	22
PinAI	ACCGGT	1 cut(s)	1983
PleI	GAGTC	3 cut(s)	62, 1415, 2885
PpsI	GAGTC	3 cut(s)	62, 1415, 2885
PscI	ACATGT	1 cut(s)	1993
PsiI	TTATAA	1 cut(s)	2508
Psp124BI	GAGCTC	1 cut(s)	924
Psp6I	CCWGG	2 cut(s)	22, 1025
PspGI	CCWGG	2 cut(s)	22, 1025
PspN4I	GGNNCC	4 cut(s)	305, 325, 1822, 2491
PspPI	GGNCC	4 cut(s)	548, 1497, 1821, 1981
PstNI	CAGNNNCTG	5 cut(s)	763, 902, 1258, 1509, 1627
PsuI	RGATCY	2 cut(s)	2044, 2548
PsyI	GACNNNGTC	1 cut(s)	173
PvuII	CAGCTG	2 cut(s)	1433, 1445
RsaI	GTAC	6 cut(s)	155, 859, 1116, 1306, 1797, 2040
RsaNI	GTAC	6 cut(s)	154, 858, 1115, 1305, 1796, 2039
RseI	CAYNNNNRTG	4 cut(s)	1319, 1998, 2244, 2312
SacI	GAGCTC	1 cut(s)	924
SacII	CCGCGG	1 cut(s)	2431
SapI	GCTCTTC	1 cut(s)	1269
Sau3AI	GATC	9 cut(s)	50, 259, 709, 1050, 1586, 1618, 1950, 2044, 2548
Sau96I	GGNCC	4 cut(s)	548, 1497, 1821, 1981
ScaI	AGTACT	1 cut(s)	1306
SchI	GAGTC	3 cut(s)	62, 1415, 2885
ScrFI	CCNGG	2 cut(s)	24, 1027
SduI	GDGCHC	8 cut(s)	42, 308, 328, 397, 522, 924, 1326, 2377
SfaNI	GCATC	7 cut(s)	82, 172, 246, 1280, 1436, 1453, 2528
SfcI	CTRYAG	2 cut(s)	444, 2738
Sfr274I	CTCGAG	1 cut(s)	219
Sfr303I	CCGCGG	1 cut(s)	2431
SgrBI	CCGCGG	1 cut(s)	2431
SinI	GGWCC	4 cut(s)	548, 1497, 1821, 1981
SlaI	CTCGAG	1 cut(s)	219
SmiMI	CAYNNNNRTG	4 cut(s)	1319, 1998, 2244, 2312
SmlI	CTYRAG	6 cut(s)	219, 1799, 1805, 2030, 2861, 3005
SmoI	CTYRAG	6 cut(s)	219, 1799, 1805, 2030, 2861, 3005
SpeI	ACTAGT	1 cut(s)	2804
SphI	GCATGC	3 cut(s)	1144, 1318, 2448
SseBI	AGGCCT	1 cut(s)	164
SspI	AATATT	3 cut(s)	1014, 2589, 3037
SspMI	CTAG	4 cut(s)	945, 984, 1005, 2805
SstI	GAGCTC	1 cut(s)	924
StuI	AGGCCT	1 cut(s)	164
StyD4I	CCNGG	2 cut(s)	22, 1025
StyI	CCWWGG	7 cut(s)	75, 108, 159, 838, 1212, 2013, 2180
TaaI	ACNGT	9 cut(s)	37, 384, 399, 629, 773, 1127, 1328, 1576, 1788
TaiI	ACGT	2 cut(s)	1120, 1227
TatI	WGTACW	4 cut(s)	857, 1304, 1795, 2038
TauI	GCSGC	3 cut(s)	116, 2295, 2433
TscAI	CASTG	8 cut(s)	154, 402, 768, 903, 1333, 1867, 2382, 2800
TseFI	GTSAC	1 cut(s)	504
Tsp45I	GTSAC	1 cut(s)	504
TspGWI	ACGGA	3 cut(s)	333, 492, 1919
TspRI	CASTG	8 cut(s)	154, 402, 768, 903, 1333, 1867, 2382, 2800
Tth111I	GACNNNGTC	1 cut(s)	173
Vha464I	CTTAAG	3 cut(s)	1799, 1805, 2861
VneI	GTGCAC	2 cut(s)	38, 1322
VpaK11BI	GGWCC	4 cut(s)	548, 1497, 1821, 1981
XagI	CCTNNNNNAGG	1 cut(s)	1024
XapI	RAATTY	8 cut(s)	647, 680, 694, 1724, 1745, 1849, 2502, 2822
XbaI	TCTAGA	1 cut(s)	944
XceI	RCATGY	5 cut(s)	1144, 1318, 1598, 1997, 2448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1465
XhoI	CTCGAG	1 cut(s)	219
XmiI	GTMKAC	1 cut(s)	1419
XmnI	GAANNNNTTC	2 cut(s)	198, 214
XspI	CTAG	4 cut(s)	945, 984, 1005, 2805
ZrmI	AGTACT	1 cut(s)	1306
Zsp2I	ATGCAT	2 cut(s)	706, 2543

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.