Rroxscaffold_2G00144460

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003928
Physical Location & Seq
Reverse (-)
82357633 .. 82360555
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144460.1

Sequence Viewer

Length: 1383 bp
ATGACCGTCGCCACCGCAGCCTCTACCCAAACCAAGAAGCTCATGAAGCTCGGCCTCTCTCTTTTCCGATGGGGCTTCAACTCCTCCAAATGCAAAACAGCGGCGAAGATGGCGGTGGCGAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATTGCTTCTCTGCTTCAGAAAGGTGAAGACGCCACTGCTCGTATCCGGGTTGAACATGTGATTAGAGAACAGAATGTTTTGGCTGCCAATGAGTTCATTGAGCTGTTCTGTGAGTTAATCGTGTCTAGACTTCAAATCATCGCTAAGCAAAGGGAGTGCCCGGCTGATCTCAAAGAAGGGATTGCTAGCTTAATCTTTGCAACACCAAGGTGCTCTGAGATTCCGGAACTGGTGGCACTAAGGAATATCTTTGAGAAGAAATATGGAAGAGATTTCGTAGCTGCTGCTACTGATTTGCGACCTGGATGTGGTGTGAATCGCATTCTCATTGACAAGCTCTCTGTTAGAACCCCTACGGGTGAAGTAAAGCTGAAAATCATGAAGGAAATAGCGAAGGAATACCAGGATGGACCGCGTAATTTTGTTAGTGCTACCAGCTTGCCTGTGACAACCCAATCTTTCGAATCTAACAAGTCAGAAACCAGGCTTTCTTCCAGTTCATTGCGCAGAACAAGTGGTGGTGGACAGAGGGAAACCAGTTCTACATGCAGAATGAGTGGTGGTGGAGAAAGGGAAACTAGTTCCTTTCGCAGAACAAGTGGTGGTGGAGAAAGAGAAACCATAACAAGTGGTGGTGGAGAAAGGGGACATTTGCAATTTGCAGACTCGGCATCAGCTGCCGCAGCAGCTGCAAAATCTGCCAGTGATGCAATGGCGGCTGCACAAGTGGCTGCATTTCTGGCCAACAAAGACTCCAATCGGGCATCTGCTAATGAATTTAAAACCCTTTCAGGCAATTCCACCAGCCAACAAGGACCAGATAGTCCAGCTCTTGACTCTTATGACATGGATCATCAATATGAAGCCGAGGGAAAAACACACTGGGCGGAGAGTTTGGACAGGTCATATTCTTCAAACAACGACGGTAACCATGGAGGAGAGGGGAAGAATTTTGTCTATAGAAGATACAGCTACAATGCTGCACCATCAGAAAATTCAGGTATCAAGTTTGATGAATCAGACACCGATGAAGAGATTGAAATGGAAGCACCTCCTAGTGGGAATTATCAAGGGCCCGAGCGTCCTCCACCACCAGTACCTTCGGCATCTGTTAGGCAGGACTCTCTTCATCGCGTTCATCCCAAATTGCCTGATTACGATACACTTGCTGCTCGCTTTGATGCCCTTAAGCATCATCACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

460

Amino Acids

50.34

Weight (kDa)

9.04

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 194 1.5e-60 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 999
Acc16I TGCGCA 1 cut(s) 683
Acc36I ACCTGC 1 cut(s) 139
AccII CGCG 2 cut(s) 592, 1311
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 7 cut(s) 15, 101, 113, 590, 858, 893, 1064
AclWI GGATC 1 cut(s) 1035
AcoI YGGCCR 1 cut(s) 918
AcsI RAATTY 3 cut(s) 953, 1126, 1171
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 1 cut(s) 1275
AfiI CCNNNNNNNGG 2 cut(s) 485, 1235
AflII CTTAAG 1 cut(s) 1364
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 79, 230, 311, 1092, 1217
AhlI ACTAGT 1 cut(s) 755
AjnI CCWGG 3 cut(s) 478, 579, 659
AleI CACNNNNGTG 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 392
AlwI GGATC 1 cut(s) 1035
AlwNI CAGNNNCTG 1 cut(s) 866
Ama87I CYCGRG 1 cut(s) 1253
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 52, 918, 1250
ApaI GGGCCC 1 cut(s) 1254
ApoI RAATTY 3 cut(s) 953, 1126, 1171
AspLEI GCGC 1 cut(s) 684
AspS9I GGNCC 4 cut(s) 587, 992, 1250, 1251
AsuC2I CCSGG 2 cut(s) 224, 338
AsuHPI GGTGA 3 cut(s) 169, 212, 548
AsuII TTCGAA 1 cut(s) 639
AsuNHI GCTAGC 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 1253
AvaII GGWCC 2 cut(s) 587, 992
BaeGI GKGCMC 2 cut(s) 338, 1254
BalI TGGCCA 1 cut(s) 920
BanII GRGCYC 1 cut(s) 1254
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 1 cut(s) 134
BccI CCATC 4 cut(s) 63, 103, 578, 1171
BcgI CGANNNNNNTGC 2 cut(s) 1325, 1359
BciT130I CCWGG 3 cut(s) 480, 581, 661
BciVI GTATCC 1 cut(s) 230
BcnI CCSGG 2 cut(s) 224, 338
BcuI ACTAGT 1 cut(s) 755
BfaI CTAG 4 cut(s) 303, 363, 756, 1233
BfmI CTRYAG 1 cut(s) 1135
BfrI CTTAAG 1 cut(s) 1364
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 230
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 5 cut(s) 224, 338, 480, 581, 661
Bme18I GGWCC 2 cut(s) 587, 992
BmeT110I CYCGRG 1 cut(s) 1253
BmgT120I GGNCC 4 cut(s) 587, 992, 1250, 1251
BmiI GGNNCC 1 cut(s) 1252
BmrFI CCNGG 5 cut(s) 224, 338, 480, 581, 661
BmrI ACTGGG 1 cut(s) 1069
BmsI GCATC 6 cut(s) 857, 874, 950, 1292, 1348, 1378
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 1069
BpiI GAAGAC 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 134
Bpu1102I GCTNAGC 1 cut(s) 321
Bpu14I TTCGAA 1 cut(s) 639
BpuMI CCSGG 2 cut(s) 224, 338
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 383, 1044, 1108
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 914, 944
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1235
Bse1I ACTGG 6 cut(s) 411, 672, 714, 879, 1064, 1271
Bse3DI GCAATG 3 cut(s) 177, 677, 894
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 3 cut(s) 480, 581, 661
BseDI CCNNGG 3 cut(s) 383, 1044, 1108
BseGI GGATG 3 cut(s) 488, 589, 1315
BseLI CCNNNNNNNGG 2 cut(s) 485, 1235
BseMI GCAATG 3 cut(s) 177, 677, 894
BseMII CTCAG 2 cut(s) 125, 384
BseNI ACTGG 6 cut(s) 411, 672, 714, 879, 1064, 1271
BseRI GAGGAG 2 cut(s) 73, 1128
BseSI GKGCMC 2 cut(s) 338, 1254
BsgI GTGCAG 2 cut(s) 882, 1143
Bsh1236I CGCG 2 cut(s) 592, 1311
BshFI GGCC 3 cut(s) 54, 920, 1252
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 1253
BsiSI CCGG 3 cut(s) 223, 338, 401
BslFI GGGAC 1 cut(s) 837
BslI CCNNNNNNNGG 2 cut(s) 485, 1235
BsmFI GGGAC 1 cut(s) 837
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 3 cut(s) 54, 920, 1252
BsoBI CYCGRG 1 cut(s) 1253
Bsp119I TTCGAA 1 cut(s) 639
Bsp120I GGGCCC 1 cut(s) 1250
Bsp1286I GDGCHC 3 cut(s) 338, 392, 1254
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 343, 1027
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 7 cut(s) 15, 101, 113, 590, 858, 893, 1064
BspANI GGCC 3 cut(s) 54, 920, 1252
BspCNI CTCAG 2 cut(s) 126, 385
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 2 cut(s) 592, 1311
BspHI TCATGA 2 cut(s) 42, 555
BspLI GGNNCC 1 cut(s) 1252
BspMI ACCTGC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 1035
BspT104I TTCGAA 1 cut(s) 639
BspTI CTTAAG 1 cut(s) 1364
BsrDI GCAATG 3 cut(s) 177, 677, 894
BsrI ACTGG 6 cut(s) 411, 672, 714, 879, 1064, 1271
BssECI CCNNGG 3 cut(s) 383, 1044, 1108
BssMI GATC 2 cut(s) 343, 1027
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 383, 1108
Bst2UI CCWGG 3 cut(s) 480, 581, 661
Bst4CI ACNGT 2 cut(s) 7, 1103
Bst6I CTCTTC 3 cut(s) 439, 1203, 1308
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 1364
BstAPI GCANNNNNTGC 3 cut(s) 854, 866, 875
BstBI TTCGAA 1 cut(s) 639
BstC8I GCNNGC 3 cut(s) 364, 617, 1351
BstDEI CTNAG 4 cut(s) 134, 321, 393, 416
BstDSI CCRYGG 1 cut(s) 1108
BstEII GGTNACC 1 cut(s) 1103
BstF5I GGATG 3 cut(s) 488, 589, 1315
BstFNI CGCG 2 cut(s) 592, 1311
BstHHI GCGC 1 cut(s) 684
BstKTI GATC 2 cut(s) 346, 1030
BstMBI GATC 2 cut(s) 343, 1027
BstNI CCWGG 3 cut(s) 480, 581, 661
BstNSI RCATGY 2 cut(s) 236, 726
BstPI GGTNACC 1 cut(s) 1103
BstSCI CCNGG 5 cut(s) 222, 336, 478, 579, 659
BstSFI CTRYAG 1 cut(s) 1135
BstSLI GKGCMC 2 cut(s) 338, 1254
BstUI CGCG 2 cut(s) 592, 1311
BstV2I GAAGAC 1 cut(s) 210
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 3 cut(s) 54, 920, 1252
BtgI CCRYGG 1 cut(s) 1108
BtgZI GCGATG 2 cut(s) 301, 1292
BtsCI GGATG 3 cut(s) 488, 589, 1315
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 3 cut(s) 210, 886, 1057
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 3 cut(s) 364, 617, 1351
CaiI CAGNNNCTG 1 cut(s) 866
CciI TCATGA 2 cut(s) 42, 555
CfoI GCGC 1 cut(s) 684
Cfr13I GGNCC 4 cut(s) 587, 992, 1250, 1251
CseI GACGC 2 cut(s) 215, 1247
Csp6I GTAC 1 cut(s) 1274
CviAII CATG 6 cut(s) 43, 233, 556, 723, 1024, 1109
CviQI GTAC 1 cut(s) 1274
DdeI CTNAG 4 cut(s) 134, 321, 393, 416
DpnI GATC 2 cut(s) 345, 1029
DpnII GATC 2 cut(s) 343, 1027
DraI TTTAAA 1 cut(s) 958
DrdI GACNNNNNNGTC 1 cut(s) 999
DseDI GACNNNNNNGTC 1 cut(s) 999
EaeI YGGCCR 1 cut(s) 918
Eam1104I CTCTTC 3 cut(s) 439, 1203, 1308
EarI CTCTTC 3 cut(s) 439, 1203, 1308
EciI GGCGGA 1 cut(s) 1079
Eco130I CCWWGG 2 cut(s) 383, 1108
Eco24I GRGCYC 1 cut(s) 1254
Eco47I GGWCC 2 cut(s) 587, 992
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 1253
Eco91I GGTNACC 1 cut(s) 1103
EcoO109I RGGNCCY 1 cut(s) 1250
EcoO65I GGTNACC 1 cut(s) 1103
EcoRII CCWGG 3 cut(s) 478, 579, 659
EcoT14I CCWWGG 2 cut(s) 383, 1108
EcoT38I GRGCYC 1 cut(s) 1254
ErhI CCWWGG 2 cut(s) 383, 1108
FaeI CATG 6 cut(s) 46, 236, 559, 726, 1027, 1112
FaqI GGGAC 1 cut(s) 837
FatI CATG 6 cut(s) 42, 232, 555, 722, 1023, 1108
FokI GGATG 3 cut(s) 495, 596, 1302
FriOI GRGCYC 1 cut(s) 1254
FspBI CTAG 4 cut(s) 303, 363, 756, 1233
FspI TGCGCA 1 cut(s) 683
GlaI GCGC 1 cut(s) 683
HaeIII GGCC 3 cut(s) 54, 920, 1252
HapII CCGG 3 cut(s) 223, 338, 401
HgaI GACGC 2 cut(s) 215, 1247
HhaI GCGC 1 cut(s) 684
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 6 cut(s) 46, 236, 559, 726, 1027, 1112
Hin6I GCGC 1 cut(s) 682
HinP1I GCGC 1 cut(s) 682
HinfI GANTC 8 cut(s) 397, 493, 641, 842, 929, 1013, 1193, 1298
HpaII CCGG 3 cut(s) 223, 338, 401
HphI GGTGA 3 cut(s) 169, 212, 548
Hpy166II GTNNAC 1 cut(s) 701
Hpy188I TCNGA 6 cut(s) 68, 195, 394, 655, 1168, 1198
Hpy188III TCNNGA 5 cut(s) 43, 303, 401, 556, 1010
Hpy8I GTNNAC 1 cut(s) 701
Hpy99I CGWCG 2 cut(s) 11, 1103
HpyAV CCTTC 4 cut(s) 347, 553, 565, 1287
HpyCH4III ACNGT 2 cut(s) 7, 1103
HpyF3I CTNAG 4 cut(s) 134, 321, 393, 416
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 6 cut(s) 46, 236, 559, 726, 1027, 1112
HspAI GCGC 1 cut(s) 682
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 2 cut(s) 343, 1027
LweI GCATC 6 cut(s) 857, 874, 950, 1292, 1348, 1378
MaeI CTAG 4 cut(s) 303, 363, 756, 1233
MaeIII GTNAC 3 cut(s) 173, 622, 1103
MalI GATC 2 cut(s) 345, 1029
MboI GATC 2 cut(s) 343, 1027
MhlI GDGCHC 3 cut(s) 338, 392, 1254
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 8 cut(s) 595, 833, 953, 973, 1126, 1171, 1240, 1322
MluNI TGGCCA 1 cut(s) 920
MlyI GAGTC 4 cut(s) 836, 923, 1007, 1292
Mox20I TGGCCA 1 cut(s) 920
MroI TCCGGA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 920
MseI TTAA 4 cut(s) 293, 368, 957, 1365
MslI CAYNNNNRTG 2 cut(s) 385, 1035
Msp20I TGGCCA 1 cut(s) 920
MspA1I CMGCKG 3 cut(s) 101, 854, 866
MspCI CTTAAG 1 cut(s) 1364
MspI CCGG 3 cut(s) 223, 338, 401
MspR9I CCNGG 5 cut(s) 224, 338, 480, 581, 661
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 3 cut(s) 480, 581, 661
MvnI CGCG 2 cut(s) 592, 1311
NciI CCSGG 2 cut(s) 224, 338
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 2 cut(s) 343, 1027
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 6 cut(s) 46, 236, 559, 726, 1027, 1112
NlaIV GGNNCC 1 cut(s) 1252
NmeAIII GCCGAG 3 cut(s) 30, 824, 1069
NmuCI GTSAC 2 cut(s) 173, 622
NsbI TGCGCA 1 cut(s) 683
NspI RCATGY 2 cut(s) 236, 726
NspV TTCGAA 1 cut(s) 639
OliI CACNNNNGTG 1 cut(s) 385
PagI TCATGA 2 cut(s) 42, 555
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 397, 493, 641, 1193
PleI GAGTC 4 cut(s) 836, 923, 1007, 1292
PpsI GAGTC 4 cut(s) 836, 923, 1007, 1292
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 478, 579, 659
PspEI GGTNACC 1 cut(s) 1103
PspGI CCWGG 3 cut(s) 478, 579, 659
PspN4I GGNNCC 1 cut(s) 1252
PspOMI GGGCCC 1 cut(s) 1250
PspPI GGNCC 4 cut(s) 587, 992, 1250, 1251
PstNI CAGNNNCTG 1 cut(s) 866
PvuII CAGCTG 2 cut(s) 854, 866
RsaI GTAC 1 cut(s) 1275
RsaNI GTAC 1 cut(s) 1274
RseI CAYNNNNRTG 2 cut(s) 385, 1035
SaqAI TTAA 4 cut(s) 293, 368, 957, 1365
Sau3AI GATC 2 cut(s) 343, 1027
Sau96I GGNCC 4 cut(s) 587, 992, 1250, 1251
SchI GAGTC 4 cut(s) 836, 923, 1007, 1292
ScrFI CCNGG 5 cut(s) 224, 338, 480, 581, 661
SduI GDGCHC 3 cut(s) 338, 392, 1254
SfaNI GCATC 6 cut(s) 857, 874, 950, 1292, 1348, 1378
SfcI CTRYAG 1 cut(s) 1135
SfuI TTCGAA 1 cut(s) 639
SinI GGWCC 2 cut(s) 587, 992
SmiMI CAYNNNNRTG 2 cut(s) 385, 1035
SmlI CTYRAG 1 cut(s) 1364
SmoI CTYRAG 1 cut(s) 1364
SpeI ACTAGT 1 cut(s) 755
Sse9I AATT 8 cut(s) 595, 833, 953, 973, 1126, 1171, 1240, 1322
SsiI CCGC 7 cut(s) 15, 101, 113, 590, 858, 893, 1064
SspMI CTAG 4 cut(s) 303, 363, 756, 1233
StyD4I CCNGG 5 cut(s) 222, 336, 478, 579, 659
StyI CCWWGG 2 cut(s) 383, 1108
TaaI ACNGT 2 cut(s) 7, 1103
TaqI TCGA 1 cut(s) 639
TasI AATT 8 cut(s) 595, 833, 953, 973, 1126, 1171, 1240, 1322
TauI GCSGC 3 cut(s) 104, 860, 896
TfiI GAWTC 4 cut(s) 397, 493, 641, 1193
Tru1I TTAA 4 cut(s) 293, 368, 957, 1365
Tru9I TTAA 4 cut(s) 293, 368, 957, 1365
TscAI CASTG 3 cut(s) 217, 886, 1064
TseFI GTSAC 2 cut(s) 173, 622
Tsp45I GTSAC 2 cut(s) 173, 622
TspRI CASTG 3 cut(s) 217, 886, 1064
Vha464I CTTAAG 1 cut(s) 1364
VpaK11BI GGWCC 2 cut(s) 587, 992
XapI RAATTY 3 cut(s) 953, 1126, 1171
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 2 cut(s) 236, 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XspI CTAG 4 cut(s) 303, 363, 756, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.