MD15G1309100.v1.1

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003928
Physical Location & Seq
Reverse (-)
31007403 .. 31007705
303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1309100.v1.1.491

Sequence Viewer

Length: 303 bp
ATGCCAATTGGTGACATGTGCTACTGTAATGGCATTCAGGATAAGGGTGGAGGCTCTAAATTCAACAAAATGCAATTGAAGAGGGCAGTGGCTCGAATAAAGCTACTGAGGAACAAGGGGCAAGTGGTAGTGAAGCAAATGAGGCGTGACATTGCCTTGCTCCTACAATTTGGTAAAAACGCTACCACTAGAATCCGAGTTGAATATGTGATCAGAGAACATAACACTTTGGCTGCCAATGAGTTCATTGAGCTCTTCTATGAATTAGTAGTATCTAGACTTTTTATCATCGCAAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

101

Amino Acids

11.53

Weight (kDa)

10.15

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 23 - 99 2e-20 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 59
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 3 cut(s) 64, 79, 203
AluBI AGCT 2 cut(s) 103, 253
AluI AGCT 2 cut(s) 103, 253
Alw21I GWGCWC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 233
ApoI RAATTY 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 23
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 220
BclI TGATCA 1 cut(s) 210
BfaI CTAG 2 cut(s) 189, 276
BisI GCNGC 1 cut(s) 234
BlsI GCNGC 1 cut(s) 235
Bse3DI GCAATG 1 cut(s) 150
BseMI GCAATG 1 cut(s) 150
BseMII CTCAG 1 cut(s) 98
BseXI GCAGC 1 cut(s) 220
BsiHKAI GWGCWC 1 cut(s) 255
BsmI GAATGC 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 1 cut(s) 210
BspCNI CTCAG 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 260
BsrDI GCAATG 1 cut(s) 150
BssMI GATC 1 cut(s) 210
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 2 cut(s) 74, 260
BstDEI CTNAG 1 cut(s) 107
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 220
BtgZI GCGATG 1 cut(s) 274
BtsI GCAGTG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 93
CviAII CATG 1 cut(s) 16
CviJI RGCY 5 cut(s) 54, 92, 103, 233, 253
CviKI_1 RGCY 5 cut(s) 54, 92, 103, 233, 253
DdeI CTNAG 1 cut(s) 107
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
Eam1104I CTCTTC 2 cut(s) 74, 260
EarI CTCTTC 2 cut(s) 74, 260
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco53kI GAGCTC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 253
EcoT38I GRGCYC 1 cut(s) 255
FaeI CATG 1 cut(s) 19
FaiI YATR 4 cut(s) 17, 207, 222, 261
FatI CATG 1 cut(s) 15
FbaI TGATCA 1 cut(s) 210
Fnu4HI GCNGC 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 234
FspBI CTAG 2 cut(s) 189, 276
GluI GCNGC 1 cut(s) 234
Hin1II CATG 1 cut(s) 19
HinfI GANTC 1 cut(s) 192
HphI GGTGA 1 cut(s) 23
Hpy188I TCNGA 2 cut(s) 197, 215
Hpy188III TCNNGA 2 cut(s) 38, 276
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4V TGCA 1 cut(s) 73
HpyF10VI GCNNNNNNNGC 1 cut(s) 142
HpyF3I CTNAG 1 cut(s) 107
Hsp92II CATG 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 210
Kzo9I GATC 1 cut(s) 210
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 1 cut(s) 165
LpnPI CCDG 1 cut(s) 23
Lsp1109I GCAGC 1 cut(s) 220
MaeI CTAG 2 cut(s) 189, 276
MaeIII GTNAC 2 cut(s) 11, 146
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 2 cut(s) 91, 247
MfeI CAATTG 2 cut(s) 6, 74
MhlI GDGCHC 1 cut(s) 255
MluCI AATT 5 cut(s) 6, 59, 74, 167, 263
MnlI CCTC 4 cut(s) 44, 75, 102, 135
MunI CAATTG 2 cut(s) 6, 74
Mva1269I GAATGC 1 cut(s) 33
MwoI GCNNNNNNNGC 1 cut(s) 142
NdeII GATC 1 cut(s) 210
NlaIII CATG 1 cut(s) 19
NmuCI GTSAC 2 cut(s) 11, 146
NspI RCATGY 1 cut(s) 19
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 1 cut(s) 33
PfeI GAWTC 1 cut(s) 192
PkrI GCNGC 1 cut(s) 235
PscI ACATGT 1 cut(s) 15
Psp124BI GAGCTC 1 cut(s) 255
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 260
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 1 cut(s) 210
SduI GDGCHC 1 cut(s) 255
SetI ASST 2 cut(s) 105, 255
Sse9I AATT 5 cut(s) 6, 59, 74, 167, 263
SspMI CTAG 2 cut(s) 189, 276
SstI GAGCTC 1 cut(s) 255
TaaI ACNGT 1 cut(s) 26
TaqI TCGA 1 cut(s) 94
TasI AATT 5 cut(s) 6, 59, 74, 167, 263
TfiI GAWTC 1 cut(s) 192
TscAI CASTG 1 cut(s) 93
TseFI GTSAC 2 cut(s) 11, 146
TseI GCWGC 1 cut(s) 233
Tsp45I GTSAC 2 cut(s) 11, 146
TspDTI ATGAA 2 cut(s) 235, 276
TspRI CASTG 1 cut(s) 93
XapI RAATTY 1 cut(s) 59
XbaI TCTAGA 1 cut(s) 275
XceI RCATGY 1 cut(s) 19
XspI CTAG 2 cut(s) 189, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.