FvH4_1g10740

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003928
Physical Location & Seq
Forward (+)
5868724 .. 5871823
3100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10740.t1

Sequence Viewer

Length: 1452 bp
ATGACTGTCGCCACCGCAGCCTCAGCCCAAACCAAGAAGATCATGAAGCTCGGCCTCTCTCTTTTTCGGCGGGGCTTCAACTCCTCCAAATGCAAAACGGCGGCGAAGATGGCGGTGGCGAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATTGCTTCTCTGCTTCAGAAAGGTGAAGACGCCACTGCTCGTATCCGGGTTGAACATGTGATTAGAGAACAGAATGTTTTGTCTGCCAATGAGTTCATTGAGCTGTTCTGTGAGTTGATCGTGTCTAGACTTCAAATCATTGCAAAGCAAAGGGAGTGTCCGGCTGACCTCAAAGAAGGAATTGCTAGCTTAATCTTTGCAACACCAAGGTGCTCTGAGATTCCAGAACTGGTGGCACTAAGGAATGTCTTTGAGAAGAAATATGGAAGAGATTTTGTAGCTGCTGCTACTGATTTGCGACCTGACTGTGGTGTGAATCGCATTCTCATTGACAAGCTCTCTGTTAGAACCCCTACGGGTGAAGTGAAGTTGAAAATAATGAAGGAAATAGCAAAGGAATACCAGGTTGATTGGGATACAGCAGAATGTGAACAAGAACTACTCAAACCTCCAGAAGAGACTATAGATGGACCTCGCAATTTTGTTAGTGCTACCAGCTTGCCTCTGACAACTCAATCTTTTGAGTCTAATAAGTCAGAAACCAGGCTTTCTTCCAGTTCGTTGCGCAGAACAAGTGCTGGTGGAGAGAGGGAAACCAGTTCTATGCGTAGAATGAGTGGAGGGGGAGAAAGGGAAACTAGTTCCTTTCACAGAACAAGTGGTGGTGGAGATAGAGAAACCAGCTCTTTTCAAAGAACAAGTAGTGGTGGAGAAAGGGGATATTTGCAATTTGCAGACTCGGCATCAGCTGCTGCAGCAGCTGCAAAATCTGCCAGCGATGCAATGGCTGCTGCACAAGTGGCTGCATTTTTGGCCAACAAAGACTCCAATCGGGCATCTGCTAATGAGTTTAAAACCCTTTCGGGCAATTCCACTGGCCAACAAGAACAAGATAGTCCAGCTGTTGACTCTTATGATATGGATCATCAATATGAAGCTGAGAGAAAAACACACTGGACTGAGAGTTTGGACAGGCCAAATTCTTCAAACAATAATGTTAGCCATGGAGGAGAGGCAAATAATTTTCTCTATAGAAGATACAGCTACAACGCACCATCAGAAAATTCAGGTATCAAGTTTGATGAATCAGACACTGATGAAGAGATTGAAATGGAGGCACCTCCAAGTGGCAATTATCACGCTCCCAAGCGTACTCCACCATCAGTACCTTCGGCAACTGTTAGGCAGGAGTCACTCCATCGTGTTCATCCCAAATTGCCCGACTACGATACACTTGCTGCTCGCTTTGATGCCCTTAAGCATCATCGCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

484

Amino Acids

53.47

Weight (kDa)

8.71

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 2e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc16I TGCGCA 1 cut(s) 743
Acc36I ACCTGC 1 cut(s) 139
AccB1I GGYRCC 1 cut(s) 1294
AciI CCGC 4 cut(s) 15, 70, 101, 113
AclWI GGATC 1 cut(s) 1107
AcoI YGGCCR 2 cut(s) 990, 1054
AcsI RAATTY 2 cut(s) 1156, 1240
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 1330, 1344
AfiI CCNNNNNNNGG 2 cut(s) 485, 1304
AflII CTTAAG 1 cut(s) 1433
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 7 cut(s) 79, 230, 311, 550, 869, 1164, 1286
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 2 cut(s) 579, 719
AleI CACNNNNGTG 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 1107
AlwNI CAGNNNCTG 3 cut(s) 929, 938, 1271
AoxI GGCC 4 cut(s) 52, 990, 1054, 1151
ApoI RAATTY 2 cut(s) 1156, 1240
ArsI GACNNNNNNTTYG 4 cut(s) 319, 351, 1126, 1158
AspLEI GCGC 1 cut(s) 744
AspS9I GGNCC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 169, 212, 548
AsuNHI GCTAGC 1 cut(s) 362
AvaII GGWCC 1 cut(s) 647
BalI TGGCCA 2 cut(s) 992, 1056
BanI GGYRCC 1 cut(s) 1294
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 2 cut(s) 22, 134
BccI CCATC 5 cut(s) 103, 638, 1240, 1345, 1383
BceAI ACGGC 1 cut(s) 114
BcgI CGANNNNNNTGC 2 cut(s) 1394, 1428
BciT130I CCWGG 2 cut(s) 581, 721
BciVI GTATCC 2 cut(s) 230, 586
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 1 cut(s) 629
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 3 cut(s) 303, 363, 816
BfmI CTRYAG 3 cut(s) 639, 930, 1207
BfrI CTTAAG 1 cut(s) 1433
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 2 cut(s) 230, 586
Bme1390I CCNGG 3 cut(s) 224, 581, 721
Bme18I GGWCC 1 cut(s) 647
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 1 cut(s) 1296
BmrFI CCNGG 3 cut(s) 224, 581, 721
BmsI GCATC 5 cut(s) 929, 946, 1022, 1417, 1447
BmtI GCTAGC 1 cut(s) 366
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 2 cut(s) 22, 134
BpuMI CCSGG 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 1098
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 383, 1180
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1304
Bse1I ACTGG 5 cut(s) 411, 732, 774, 1057, 1136
Bse3DI GCAATG 3 cut(s) 177, 315, 966
Bse8I GATNNNNATC 1 cut(s) 1098
BseBI CCWGG 2 cut(s) 581, 721
BseDI CCNNGG 2 cut(s) 383, 1180
BseGI GGATG 1 cut(s) 1384
BseJI GATNNNNATC 1 cut(s) 1098
BseLI CCNNNNNNNGG 2 cut(s) 485, 1304
BseMI GCAATG 3 cut(s) 177, 315, 966
BseMII CTCAG 5 cut(s) 36, 125, 384, 1107, 1128
BseNI ACTGG 5 cut(s) 411, 732, 774, 1057, 1136
BseRI GAGGAG 2 cut(s) 73, 1200
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 4 cut(s) 54, 992, 1056, 1153
BshNI GGYRCC 1 cut(s) 1294
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 2 cut(s) 223, 338
BslI CCNNNNNNNGG 2 cut(s) 485, 1304
BsmAI GTCTC 1 cut(s) 629
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 4 cut(s) 54, 992, 1056, 1153
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 3 cut(s) 39, 294, 1099
Bsp19I CCATGG 1 cut(s) 1180
BspACI CCGC 4 cut(s) 15, 70, 101, 113
BspANI GGCC 4 cut(s) 54, 992, 1056, 1153
BspCNI CTCAG 5 cut(s) 35, 126, 385, 1108, 1129
BspHI TCATGA 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 1296
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 1107
BspT107I GGYRCC 1 cut(s) 1294
BspTI CTTAAG 1 cut(s) 1433
BsrDI GCAATG 3 cut(s) 177, 315, 966
BsrI ACTGG 5 cut(s) 411, 732, 774, 1057, 1136
BssECI CCNNGG 2 cut(s) 383, 1180
BssMI GATC 3 cut(s) 39, 294, 1099
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 383, 1180
Bst2UI CCWGG 2 cut(s) 581, 721
Bst4CI ACNGT 3 cut(s) 7, 485, 1357
Bst6I CTCTTC 3 cut(s) 439, 627, 1272
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 1433
BstAPI GCANNNNNTGC 4 cut(s) 926, 938, 947, 965
BstC8I GCNNGC 4 cut(s) 364, 677, 952, 1420
BstDEI CTNAG 6 cut(s) 22, 134, 393, 416, 1116, 1137
BstDSI CCRYGG 1 cut(s) 1180
BstF5I GGATG 1 cut(s) 1384
BstHHI GCGC 1 cut(s) 744
BstKTI GATC 3 cut(s) 42, 297, 1102
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 3 cut(s) 39, 294, 1099
BstNI CCWGG 2 cut(s) 581, 721
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 3 cut(s) 222, 579, 719
BstSFI CTRYAG 3 cut(s) 639, 930, 1207
BstV2I GAAGAC 1 cut(s) 210
BsuI GTATCC 2 cut(s) 230, 586
BsuRI GGCC 4 cut(s) 54, 992, 1056, 1153
BtgI CCRYGG 1 cut(s) 1180
BtgZI GCGATG 2 cut(s) 969, 1427
BtsCI GGATG 1 cut(s) 1384
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 4 cut(s) 210, 1050, 1129, 1269
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 4 cut(s) 364, 677, 952, 1420
CaiI CAGNNNCTG 3 cut(s) 929, 938, 1271
CciI TCATGA 1 cut(s) 42
CfoI GCGC 1 cut(s) 744
Cfr13I GGNCC 1 cut(s) 647
CseI GACGC 1 cut(s) 215
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 2 cut(s) 1329, 1343
CviAII CATG 3 cut(s) 43, 233, 1181
CviQI GTAC 2 cut(s) 1329, 1343
DdeI CTNAG 6 cut(s) 22, 134, 393, 416, 1116, 1137
DpnI GATC 3 cut(s) 41, 296, 1101
DpnII GATC 3 cut(s) 39, 294, 1099
DraI TTTAAA 1 cut(s) 1030
EaeI YGGCCR 2 cut(s) 990, 1054
Eam1104I CTCTTC 3 cut(s) 439, 627, 1272
EarI CTCTTC 3 cut(s) 439, 627, 1272
Eco130I CCWWGG 2 cut(s) 383, 1180
Eco47I GGWCC 1 cut(s) 647
Eco57I CTGAAG 1 cut(s) 176
EcoRII CCWGG 2 cut(s) 579, 719
EcoT14I CCWWGG 2 cut(s) 383, 1180
ErhI CCWWGG 2 cut(s) 383, 1180
FaeI CATG 3 cut(s) 46, 236, 1184
FatI CATG 3 cut(s) 42, 232, 1180
FauI CCCGC 1 cut(s) 63
FokI GGATG 1 cut(s) 1371
FspBI CTAG 3 cut(s) 303, 363, 816
FspI TGCGCA 1 cut(s) 743
GlaI GCGC 1 cut(s) 743
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 4 cut(s) 54, 992, 1056, 1153
HapII CCGG 2 cut(s) 223, 338
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 744
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 3 cut(s) 46, 236, 1184
Hin6I GCGC 1 cut(s) 742
HinP1I GCGC 1 cut(s) 742
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HinfI GANTC 8 cut(s) 397, 493, 701, 914, 1001, 1085, 1262, 1367
HpaII CCGG 2 cut(s) 223, 338
HphI GGTGA 3 cut(s) 169, 212, 548
Hpy166II GTNNAC 2 cut(s) 608, 1084
Hpy188I TCNGA 6 cut(s) 195, 394, 684, 715, 1237, 1267
Hpy188III TCNNGA 4 cut(s) 43, 303, 401, 629
Hpy8I GTNNAC 2 cut(s) 608, 1084
HpyAV CCTTC 3 cut(s) 347, 553, 1356
HpyCH4III ACNGT 3 cut(s) 7, 485, 1357
HpyF3I CTNAG 6 cut(s) 22, 134, 393, 416, 1116, 1137
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 3 cut(s) 46, 236, 1184
HspAI GCGC 1 cut(s) 742
Kzo9I GATC 3 cut(s) 39, 294, 1099
LmnI GCTCC 1 cut(s) 1324
LweI GCATC 5 cut(s) 929, 946, 1022, 1417, 1447
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 3 cut(s) 303, 363, 816
MaeIII GTNAC 2 cut(s) 173, 1368
MalI GATC 3 cut(s) 41, 296, 1101
MboI GATC 3 cut(s) 39, 294, 1099
MhlI GDGCHC 1 cut(s) 392
MlsI TGGCCA 2 cut(s) 992, 1056
MluCI AATT 9 cut(s) 357, 655, 905, 1045, 1156, 1198, 1240, 1309, 1391
MluNI TGGCCA 2 cut(s) 992, 1056
MlyI GAGTC 5 cut(s) 710, 908, 995, 1079, 1376
Mox20I TGGCCA 2 cut(s) 992, 1056
MscI TGGCCA 2 cut(s) 992, 1056
MseI TTAA 3 cut(s) 368, 1029, 1434
MslI CAYNNNNRTG 2 cut(s) 385, 1107
Msp20I TGGCCA 2 cut(s) 992, 1056
MspA1I CMGCKG 3 cut(s) 926, 938, 1079
MspCI CTTAAG 1 cut(s) 1433
MspI CCGG 2 cut(s) 223, 338
MspR9I CCNGG 3 cut(s) 224, 581, 721
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 2 cut(s) 581, 721
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 1180
NdeII GATC 3 cut(s) 39, 294, 1099
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 3 cut(s) 46, 236, 1184
NlaIV GGNNCC 1 cut(s) 1296
NmeAIII GCCGAG 2 cut(s) 30, 896
NmuCI GTSAC 2 cut(s) 173, 1368
NsbI TGCGCA 1 cut(s) 743
NspI RCATGY 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 385
PagI TCATGA 1 cut(s) 42
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 3 cut(s) 397, 493, 1262
PleI GAGTC 5 cut(s) 709, 908, 995, 1079, 1375
PpsI GAGTC 5 cut(s) 709, 908, 995, 1079, 1375
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 2 cut(s) 579, 719
PspGI CCWGG 2 cut(s) 579, 719
PspN4I GGNNCC 1 cut(s) 1296
PspPI GGNCC 1 cut(s) 647
PsrI GAACNNNNNNTAC 2 cut(s) 600, 632
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 3 cut(s) 929, 938, 1271
PvuII CAGCTG 3 cut(s) 926, 938, 1079
RsaI GTAC 2 cut(s) 1330, 1344
RsaNI GTAC 2 cut(s) 1329, 1343
RseI CAYNNNNRTG 2 cut(s) 385, 1107
SaqAI TTAA 3 cut(s) 368, 1029, 1434
Sau3AI GATC 3 cut(s) 39, 294, 1099
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 5 cut(s) 710, 908, 995, 1079, 1376
ScrFI CCNGG 3 cut(s) 224, 581, 721
SduI GDGCHC 1 cut(s) 392
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 5 cut(s) 929, 946, 1022, 1417, 1447
SfcI CTRYAG 3 cut(s) 639, 930, 1207
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 2 cut(s) 385, 1107
SmlI CTYRAG 1 cut(s) 1433
SmoI CTYRAG 1 cut(s) 1433
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 9 cut(s) 357, 655, 905, 1045, 1156, 1198, 1240, 1309, 1391
SsiI CCGC 4 cut(s) 15, 70, 101, 113
SspMI CTAG 3 cut(s) 303, 363, 816
StyD4I CCNGG 3 cut(s) 222, 579, 719
StyI CCWWGG 2 cut(s) 383, 1180
TaaI ACNGT 3 cut(s) 7, 485, 1357
TasI AATT 9 cut(s) 357, 655, 905, 1045, 1156, 1198, 1240, 1309, 1391
TauI GCSGC 1 cut(s) 104
TfiI GAWTC 3 cut(s) 397, 493, 1262
Tru1I TTAA 3 cut(s) 368, 1029, 1434
Tru9I TTAA 3 cut(s) 368, 1029, 1434
TscAI CASTG 4 cut(s) 217, 1057, 1136, 1276
TseFI GTSAC 2 cut(s) 173, 1368
Tsp45I GTSAC 2 cut(s) 173, 1368
TspDTI ATGAA 7 cut(s) 59, 262, 572, 1125, 1275, 1290, 1373
TspRI CASTG 4 cut(s) 217, 1057, 1136, 1276
Vha464I CTTAAG 1 cut(s) 1433
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 2 cut(s) 1156, 1240
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 958
XspI CTAG 3 cut(s) 303, 363, 816
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.