Rorug02G0069100

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003928
Physical Location & Seq
Reverse (-)
5382718 .. 5385747
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0069100.1

Sequence Viewer

Length: 657 bp
ATGTTTACCAGGCTTTTTGGAAAACCGAAAACGGAATCCAGTGCCCTAACCACCTTAGACAAGTTAAATGAGACGCTTGAAATGCTTGAGAAAAAGGAGAAAGTACTACAGAAGAAGGCTGCCGCTGAAGTTGAGAGGGCCAAGGAGTTCACCAGAGCAAAGAATAAACGATCTGCTATACAATGTTTGAAGAGGAAGAAGCTGTACGAACAGCAAATAGAGCAGCTTGGAAACTTCCAACTCCGAATCCATGATCAGATGATAATGCTAGAAGGTGCAAAAGCCACTACTGAAACTGTAGATGCCTTGAGAACTGGAGCAGCTGCCATGAAGGCAATGCAGAAAGCAACGAACATAGATGACGTGGACAAGACCATGGATGAGATTAATGAGCAGACCGAGAACATGAAACAGATCCAGGAAGCTTTGTCAACTCCAATTGGTTCAGCTGCTGATTTTGATGAGGATGAATTGGAAGCAGAACTTGAAGAGCTGGAAGGTGCTGAGTTGGAAGAACAACTTCTTCAGCCTGCAACTACAGCGCCTGCAGCTCCAGTGCATGTCCCAGAAGGAAGGCAACCAACACGTCCTGTTCCCCAGAAGCGCACTGCCGAAGAAGATGAACTGGCTGCTTTGCAGGCTGAGATGGCACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

218

Amino Acids

24.4

Weight (kDa)

4.86

Isoelectric Point (pI)

51.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Snf7 PF03357 20 - 186 2.1e-47 Snf7
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 123
AclWI GGATC 1 cut(s) 409
AcuI CTGAAG 2 cut(s) 147, 509
AfaI GTAC 2 cut(s) 105, 206
AflIII ACRYGT 1 cut(s) 584
AgsI TTSAA 3 cut(s) 80, 190, 488
AjiI CACGTC 2 cut(s) 364, 587
AjnI CCWGG 2 cut(s) 8, 417
AluBI AGCT 7 cut(s) 202, 226, 323, 425, 449, 493, 551
AluI AGCT 7 cut(s) 202, 226, 323, 425, 449, 493, 551
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 1 cut(s) 409
AlwNI CAGNNNCTG 2 cut(s) 452, 545
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 7 cut(s) 119, 223, 320, 323, 449, 548, 629
AseI ATTAAT 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 2 cut(s) 544, 606
AspS9I GGNCC 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 142
BaeGI GKGCMC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 188, 220
BbvI GCAGC 7 cut(s) 106, 235, 310, 332, 436, 560, 616
BccI CCATC 1 cut(s) 640
BciT130I CCWGG 2 cut(s) 10, 419
BclI TGATCA 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 65
BfaI CTAG 1 cut(s) 269
BfmI CTRYAG 4 cut(s) 107, 297, 537, 546
BfoI RGCGCY 1 cut(s) 545
BglI GCCNNNNNGGC 1 cut(s) 332
BisI GCNGC 8 cut(s) 120, 123, 224, 321, 324, 450, 549, 630
BlsI GCNGC 8 cut(s) 121, 124, 225, 322, 325, 451, 550, 631
BmcAI AGTACT 1 cut(s) 105
Bme1390I CCNGG 2 cut(s) 10, 419
BmgBI CACGTC 2 cut(s) 364, 587
BmgT120I GGNCC 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 10, 419
BmsI GCATC 1 cut(s) 292
BpmI CTGGAG 2 cut(s) 336, 537
BpuEI CTTGAG 2 cut(s) 107, 328
BsaJI CCNNGG 2 cut(s) 141, 375
Bse1I ACTGG 4 cut(s) 39, 319, 554, 630
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 2 cut(s) 10, 419
BseDI CCNNGG 2 cut(s) 141, 375
BseGI GGATG 2 cut(s) 385, 472
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 495, 633
BseNI ACTGG 4 cut(s) 39, 319, 554, 630
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 7 cut(s) 106, 235, 310, 332, 436, 560, 616
BshFI GGCC 1 cut(s) 140
BslFI GGGAC 1 cut(s) 548
BsmAI GTCTC 1 cut(s) 65
BsmBI CGTCTC 1 cut(s) 65
BsmFI GGGAC 1 cut(s) 548
BsnI GGCC 1 cut(s) 140
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 3 cut(s) 170, 253, 414
Bsp19I CCATGG 1 cut(s) 375
BspACI CCGC 1 cut(s) 123
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 2 cut(s) 496, 634
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 483
BsrDI GCAATG 1 cut(s) 342
BsrI ACTGG 4 cut(s) 39, 319, 554, 630
BssECI CCNNGG 2 cut(s) 141, 375
BssMI GATC 3 cut(s) 170, 253, 414
BssT1I CCWWGG 2 cut(s) 141, 375
Bst2UI CCWGG 2 cut(s) 10, 419
Bst4CI ACNGT 1 cut(s) 298
Bst6I CTCTTC 2 cut(s) 185, 483
BstC8I GCNNGC 3 cut(s) 531, 546, 639
BstDEI CTNAG 3 cut(s) 55, 504, 642
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 2 cut(s) 385, 472
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 544, 606
BstKTI GATC 3 cut(s) 173, 256, 417
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 3 cut(s) 170, 253, 414
BstMWI GCNNNNNNNGC 7 cut(s) 82, 220, 332, 539, 548, 638, 647
BstNI CCWGG 2 cut(s) 10, 419
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 2 cut(s) 8, 417
BstSFI CTRYAG 4 cut(s) 107, 297, 537, 546
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 7 cut(s) 106, 235, 310, 332, 436, 560, 616
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 1 cut(s) 375
BtrI CACGTC 2 cut(s) 364, 587
BtsCI GGATG 2 cut(s) 385, 472
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 3 cut(s) 46, 561, 606
Cac8I GCNNGC 3 cut(s) 531, 546, 639
CaiI CAGNNNCTG 2 cut(s) 452, 545
CfoI GCGC 2 cut(s) 544, 606
Cfr13I GGNCC 1 cut(s) 138
CseI GACGC 1 cut(s) 82
Csp6I GTAC 2 cut(s) 104, 205
CviAII CATG 5 cut(s) 251, 328, 376, 406, 560
CviQI GTAC 2 cut(s) 104, 205
DdeI CTNAG 3 cut(s) 55, 504, 642
DpnI GATC 3 cut(s) 172, 255, 416
DpnII GATC 3 cut(s) 170, 253, 414
Eam1104I CTCTTC 2 cut(s) 185, 483
EarI CTCTTC 2 cut(s) 185, 483
Eco130I CCWWGG 2 cut(s) 141, 375
Eco57I CTGAAG 2 cut(s) 147, 509
EcoRII CCWGG 2 cut(s) 8, 417
EcoT14I CCWWGG 2 cut(s) 141, 375
ErhI CCWWGG 2 cut(s) 141, 375
Esp3I CGTCTC 1 cut(s) 65
FaeI CATG 5 cut(s) 254, 331, 379, 409, 563
FaiI YATR 8 cut(s) 179, 252, 329, 356, 377, 407, 561, 655
FalI AAGNNNNNCTT 4 cut(s) 468, 500, 504, 536
FaqI GGGAC 1 cut(s) 548
FatI CATG 5 cut(s) 250, 327, 375, 405, 559
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 8 cut(s) 120, 123, 224, 321, 324, 450, 549, 630
FokI GGATG 2 cut(s) 392, 479
Fsp4HI GCNGC 8 cut(s) 120, 123, 224, 321, 324, 450, 549, 630
FspBI CTAG 1 cut(s) 269
GlaI GCGC 2 cut(s) 543, 605
GluI GCNGC 8 cut(s) 120, 123, 224, 321, 324, 450, 549, 630
GsuI CTGGAG 2 cut(s) 336, 537
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 1 cut(s) 140
HgaI GACGC 1 cut(s) 82
HhaI GCGC 2 cut(s) 544, 606
Hin1II CATG 5 cut(s) 254, 331, 379, 409, 563
Hin6I GCGC 2 cut(s) 542, 604
HinP1I GCGC 2 cut(s) 542, 604
HincII GTYRAC 1 cut(s) 432
HindII GTYRAC 1 cut(s) 432
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 2 cut(s) 35, 246
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 4 cut(s) 6, 150, 367, 432
Hpy188I TCNGA 2 cut(s) 245, 258
Hpy8I GTNNAC 4 cut(s) 6, 150, 367, 432
HpyAV CCTTC 6 cut(s) 109, 266, 325, 491, 563, 567
HpyCH4III ACNGT 1 cut(s) 298
HpyCH4IV ACGT 2 cut(s) 363, 586
HpyCH4V TGCA 6 cut(s) 278, 340, 533, 548, 559, 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 82, 220, 332, 539, 548, 638, 647
HpyF3I CTNAG 3 cut(s) 55, 504, 642
HpySE526I ACGT 2 cut(s) 363, 586
Hsp92II CATG 5 cut(s) 254, 331, 379, 409, 563
HspAI GCGC 2 cut(s) 542, 604
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 170, 253, 414
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 2 cut(s) 317, 556
Lsp1109I GCAGC 7 cut(s) 106, 235, 310, 332, 436, 560, 616
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 269
MaeII ACGT 2 cut(s) 363, 586
MalI GATC 3 cut(s) 172, 255, 416
MboI GATC 3 cut(s) 170, 253, 414
MboII GAAGA 8 cut(s) 124, 202, 208, 500, 515, 524, 626, 629
MfeI CAATTG 1 cut(s) 438
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 2 cut(s) 438, 470
MmeI TCCRAC 2 cut(s) 262, 489
MnlI CCTC 3 cut(s) 129, 186, 457
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 2 cut(s) 65, 387
MspA1I CMGCKG 3 cut(s) 125, 323, 449
MspR9I CCNGG 2 cut(s) 10, 419
MunI CAATTG 1 cut(s) 438
MvaI CCWGG 2 cut(s) 10, 419
MwoI GCNNNNNNNGC 7 cut(s) 82, 220, 332, 539, 548, 638, 647
NcoI CCATGG 1 cut(s) 375
NdeII GATC 3 cut(s) 170, 253, 414
NlaIII CATG 5 cut(s) 254, 331, 379, 409, 563
NspI RCATGY 1 cut(s) 563
PciSI GCTCTTC 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 35, 246
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 8 cut(s) 121, 124, 225, 322, 325, 451, 550, 631
PshBI ATTAAT 1 cut(s) 387
Psp6I CCWGG 2 cut(s) 8, 417
PspGI CCWGG 2 cut(s) 8, 417
PspPI GGNCC 1 cut(s) 138
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 2 cut(s) 452, 545
PsuI RGATCY 1 cut(s) 414
PvuII CAGCTG 2 cut(s) 323, 449
RsaI GTAC 2 cut(s) 105, 206
RsaNI GTAC 2 cut(s) 104, 205
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 2 cut(s) 65, 387
SatI GCNGC 8 cut(s) 120, 123, 224, 321, 324, 450, 549, 630
Sau3AI GATC 3 cut(s) 170, 253, 414
Sau96I GGNCC 1 cut(s) 138
ScaI AGTACT 1 cut(s) 105
ScrFI CCNGG 2 cut(s) 10, 419
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 1 cut(s) 292
SfcI CTRYAG 4 cut(s) 107, 297, 537, 546
SmlI CTYRAG 2 cut(s) 86, 307
SmoI CTYRAG 2 cut(s) 86, 307
Sse9I AATT 2 cut(s) 438, 470
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 8, 417
StyI CCWWGG 2 cut(s) 141, 375
TaaI ACNGT 1 cut(s) 298
TaiI ACGT 2 cut(s) 366, 589
TaqII GACCGA 1 cut(s) 413
TasI AATT 2 cut(s) 438, 470
TatI WGTACW 1 cut(s) 103
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 2 cut(s) 35, 246
Tru1I TTAA 2 cut(s) 65, 387
Tru9I TTAA 2 cut(s) 65, 387
TscAI CASTG 3 cut(s) 46, 561, 613
TseI GCWGC 7 cut(s) 119, 223, 320, 323, 449, 548, 629
TspDTI ATGAA 4 cut(s) 344, 422, 483, 636
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 3 cut(s) 46, 561, 613
VspI ATTAAT 1 cut(s) 387
XceI RCATGY 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 1 cut(s) 269
ZrmI AGTACT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.