pycom08g13410

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003928
Physical Location & Seq
Forward (+)
12197753 .. 12198289
537 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13410.1

Sequence Viewer

Length: 537 bp
ATGCAATTTAAGATTGCAGTGGCTCAAATAAAGCTGCTGAGGAACAAGAGGCAGGCGGTGGTGAAGCAAATGAGGCGTGACTTTACCTTGCTCCTACAATCTGGTCAAGGTGCCACTGCTCGAATCTGGGTTGAACATCTGTTTAGAGAACAGAAAAATTTAGCCGTCAATGAGTTCATTGAGCTCTTTTGTGAATTAGTAGTATCTAGACTTTCTATCATCACAAAGCAAATGGAGTGTCCACTTAATTTCAAAGAGGGAATTGCTAGTCTAATCTTTGCCTCTTCTAGGTGTTATGAGATTTCTGAACTGATCACAGTAAGGAATATTTTTGAGGAAAAGTATGGTAAAGATTTTGTATTTGTAGCTGTCGATGTACGTCCCAACTGCGATGTGAATTGCATGATGTGGTGTGAAAGAGGACATATGCAGTTTGTAGACTCAACATCAGCTACTGAAGTAGCTGCAAAATCTGCCAGTGATGCAATGGCTGCTACACAAGTTGCTACATATTTGGCTAACACAGACTGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

179

Amino Acids

20.25

Weight (kDa)

7.55

Isoelectric Point (pI)

36.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 2 - 136 2.6e-40 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 438
AciI CCGC 1 cut(s) 56
AcsI RAATTY 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 288
AgsI TTSAA 2 cut(s) 134, 253
AluBI AGCT 5 cut(s) 34, 184, 368, 452, 464
AluI AGCT 5 cut(s) 34, 184, 368, 452, 464
Alw21I GWGCWC 1 cut(s) 186
AlwNI CAGNNNCTG 1 cut(s) 455
ApeKI GCWGC 3 cut(s) 34, 464, 491
ApoI RAATTY 1 cut(s) 157
ArsI GACNNNNNNTTYG 2 cut(s) 223, 255
AsuHPI GGTGA 1 cut(s) 73
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvCI CCTCAGC 1 cut(s) 38
BbvI GCAGC 3 cut(s) 21, 451, 478
BceAI ACGGC 1 cut(s) 149
BclI TGATCA 1 cut(s) 312
BfaI CTAG 3 cut(s) 207, 267, 288
BisI GCNGC 3 cut(s) 35, 465, 492
BlsI GCNGC 3 cut(s) 36, 466, 493
BmiI GGNNCC 1 cut(s) 112
BmsI GCATC 1 cut(s) 472
Bpu10I CCTNAGC 1 cut(s) 38
Bsc4I CCNNNNNNNGG 1 cut(s) 288
Bse1I ACTGG 1 cut(s) 477
Bse3DI GCAATG 1 cut(s) 492
BseLI CCNNNNNNNGG 1 cut(s) 288
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 1 cut(s) 29
BseNI ACTGG 1 cut(s) 477
BseXI GCAGC 3 cut(s) 21, 451, 478
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 186
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 366
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 312
BspACI CCGC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 30
BspLI GGNNCC 1 cut(s) 112
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 1 cut(s) 477
BssMI GATC 1 cut(s) 312
Bst4CI ACNGT 1 cut(s) 319
Bst6I CTCTTC 1 cut(s) 289
BstAPI GCANNNNNTGC 2 cut(s) 473, 491
BstC8I GCNNGC 1 cut(s) 54
BstDEI CTNAG 1 cut(s) 38
BstENI CCTNNNNNAGG 1 cut(s) 286
BstKTI GATC 1 cut(s) 315
BstMBI GATC 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 73, 473, 482, 491
BstV1I GCAGC 3 cut(s) 21, 451, 478
BtgZI GCGATG 1 cut(s) 405
BtsI GCAGTG 2 cut(s) 24, 114
BtsIMutI CAGTG 3 cut(s) 24, 114, 484
Cac8I GCNNGC 1 cut(s) 54
CaiI CAGNNNCTG 1 cut(s) 455
Csp6I GTAC 1 cut(s) 377
CviAII CATG 1 cut(s) 403
CviJI RGCY 9 cut(s) 23, 34, 164, 184, 368, 452, 464, 491, 518
CviKI_1 RGCY 9 cut(s) 23, 34, 164, 184, 368, 452, 464, 491, 518
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 1 cut(s) 38
DpnI GATC 1 cut(s) 314
DpnII GATC 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Ecl136II GAGCTC 1 cut(s) 184
Eco24I GRGCYC 1 cut(s) 186
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 477
EcoICRI GAGCTC 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 286
EcoT38I GRGCYC 1 cut(s) 186
FaeI CATG 1 cut(s) 406
FaiI YATR 6 cut(s) 297, 345, 404, 426, 428, 511
FaqI GGGAC 1 cut(s) 366
FatI CATG 1 cut(s) 402
FauNDI CATATG 1 cut(s) 426
FbaI TGATCA 1 cut(s) 312
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 3 cut(s) 35, 465, 492
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 35, 465, 492
FspBI CTAG 3 cut(s) 207, 267, 288
GluI GCNGC 3 cut(s) 35, 465, 492
Hin1II CATG 1 cut(s) 406
HinfI GANTC 2 cut(s) 123, 440
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 242, 439
Hpy188I TCNGA 1 cut(s) 307
Hpy188III TCNNGA 1 cut(s) 207
Hpy8I GTNNAC 2 cut(s) 242, 439
HpyCH4III ACNGT 1 cut(s) 319
HpyCH4IV ACGT 1 cut(s) 379
HpyCH4V TGCA 7 cut(s) 4, 17, 402, 430, 467, 485, 531
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 473, 482, 491
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 379
Hsp92II CATG 1 cut(s) 406
Ksp22I TGATCA 1 cut(s) 312
Kzo9I GATC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 4 cut(s) 38, 87, 112, 490
Lsp1109I GCAGC 3 cut(s) 21, 451, 478
LweI GCATC 1 cut(s) 472
MaeI CTAG 3 cut(s) 207, 267, 288
MaeII ACGT 1 cut(s) 379
MaeIII GTNAC 1 cut(s) 77
MalI GATC 1 cut(s) 314
MboI GATC 1 cut(s) 312
MboII GAAGA 1 cut(s) 276
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 7 cut(s) 5, 157, 194, 247, 261, 397, 532
MlyI GAGTC 1 cut(s) 434
MnlI CCTC 7 cut(s) 33, 42, 66, 250, 292, 328, 413
MseI TTAA 2 cut(s) 9, 246
MwoI GCNNNNNNNGC 4 cut(s) 73, 473, 482, 491
NdeI CATATG 1 cut(s) 426
NdeII GATC 1 cut(s) 312
NlaIII CATG 1 cut(s) 406
NlaIV GGNNCC 1 cut(s) 112
NmuCI GTSAC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 123
PkrI GCNGC 3 cut(s) 36, 466, 493
PleI GAGTC 1 cut(s) 434
PpsI GAGTC 1 cut(s) 434
Psp124BI GAGCTC 1 cut(s) 186
PspN4I GGNNCC 1 cut(s) 112
PstNI CAGNNNCTG 1 cut(s) 455
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 2 cut(s) 9, 246
SatI GCNGC 3 cut(s) 35, 465, 492
Sau3AI GATC 1 cut(s) 312
SchI GAGTC 1 cut(s) 434
SduI GDGCHC 1 cut(s) 186
SetI ASST 9 cut(s) 36, 89, 112, 186, 293, 370, 382, 454, 466
SfaNI GCATC 1 cut(s) 472
Sse9I AATT 7 cut(s) 5, 157, 194, 247, 261, 397, 532
SsiI CCGC 1 cut(s) 56
SspI AATATT 1 cut(s) 328
SspMI CTAG 3 cut(s) 207, 267, 288
SstI GAGCTC 1 cut(s) 186
TaaI ACNGT 1 cut(s) 319
TaiI ACGT 1 cut(s) 382
TaqI TCGA 2 cut(s) 121, 372
TasI AATT 7 cut(s) 5, 157, 194, 247, 261, 397, 532
TfiI GAWTC 1 cut(s) 123
Tru1I TTAA 2 cut(s) 9, 246
Tru9I TTAA 2 cut(s) 9, 246
TscAI CASTG 3 cut(s) 24, 121, 484
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 3 cut(s) 34, 464, 491
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 1 cut(s) 166
TspRI CASTG 3 cut(s) 24, 121, 484
XagI CCTNNNNNAGG 1 cut(s) 286
XapI RAATTY 1 cut(s) 157
XbaI TCTAGA 1 cut(s) 206
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 3 cut(s) 207, 267, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.