RLG00000016744

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0003928
Physical Location & Seq
Forward (+)
9638955 .. 9641781
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016744

Sequence Viewer

Length: 1449 bp
ATGACCGTCGCCACCGCAGCCTCAACCCAAACCAAGAAGCTCATGAAGCTCGGCCTCTCTCTTTTCCGATGGGGCTTCAACTCCTCCAAATGCAAAACAGCGGCGAAGATGGCGGTGGCGAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATTGCTTCTCTGCTTCAGAAAGGTGAAGACGCCACTGCTCGTATCCGGGTTGAACATGTGATTAGAGAACAGAATGTTTTGGCTGCCAATGAGTTCATTGAGCTGTTCTGTGAGTTAATTGTGTCTAGACTTCAAATCATCGCTAAGCAGAGGGAGTGCCCGGCTGATCTCAAAGAAGGGATTGCTAGCTTAATCTTTGCAACACCAAGGTGCTCTGAGATTCCGGAACTGGTGGCACTAAGGAATATCTTTGAGAAGAAATATGGAAGAGATTTCGTAGCTGCTGCTACTGATTTGCGACCTGGATGTGGTGTGAATCGCATTCTCATTGACAAGCTCTCTGTTAGAACCCCTACGGGTGAAGTAAAGCTGAAAATCATGAAGGAAATAGCGAAGGAATACCAGGTTGATTGGGATACAACAGAATCTGAACAAGAACTTCTCAAACCTCCAGAAGAGACACTAGATGGACCGCGTACTTTTGTTAGTGCTACCAGCTTGCCTGTGACAACCCAATCTTTTGAGTCTAACAAGTCAGAAACCAGGCTTTCTTCCAGTTCGTTGCGCAGAACAAGTGGTGATGGACAGAGGGAAACCAGTTCTACAAGCAGAATGAGTGGTGGTGGAGAAAGGGAAACTAGTTCCTTTCGCAGAACAAGCGGTGGTGGAGAAAGAGAAACCAGAACAAGTGGTGGTGGAGAAAGAGGACATTTGCAATTTGCAGACTCGGCATCAGCTGCAGCAGCAGCTGCAAAATCTGCCAGTGATGCAATGGCGGCTGCACAAGTGGCCGCATTTCTGGCTAACAAAGACTCCAATCGGGCATCTGCATCTGCTAATGAATTTAAAACCCTTTCGACCAATTCCACCGGCCAACAAGGACCGGATAGTCCAGCTCTTGACTCTTATGACATGGATCATCAATATGAAGCTGAGGGAAAAACACACTGGGCGGAGAGTTTGGATAGGTCATATTCTTCAAATAAGGACGTTAACCATGGAGGAGAGGGGAAGAATTTTGTCTATAGAAGATACAGCTACAATGCTGCACCCTCAGAAAATTCAGGTATCAAGTTTGATGAATCAGACGCTGATGAAGAGATTGAAATGGAAGCACCTCCTAGTGGGAATTATCAAGGGCCGGAGCGTCCCCCACCACTAGTACCATCGGGAACTGTTAGGCAGGACTCACTTCATCGCGTTCATCCCAAATTGCCTGATTACGATACACTTGCTGCTCGCTTTGATGCCCTTAAGCATCATCACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

483

Amino Acids

52.97

Weight (kDa)

8.41

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 2.1e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 1065
Acc16I TGCGCA 1 cut(s) 743
Acc36I ACCTGC 1 cut(s) 139
AccII CGCG 2 cut(s) 652, 1377
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 8 cut(s) 15, 101, 113, 650, 837, 953, 969, 1130
AclWI GGATC 1 cut(s) 1101
AcoI YGGCCR 2 cut(s) 966, 1048
AcsI RAATTY 3 cut(s) 1019, 1192, 1237
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 655, 1341
AfiI CCNNNNNNNGG 2 cut(s) 485, 1301
AflII CTTAAG 1 cut(s) 1430
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 79, 230, 311, 1158, 1283
AhlI ACTAGT 2 cut(s) 815, 1336
AjnI CCWGG 3 cut(s) 478, 579, 719
AleI CACNNNNGTG 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 1101
AlwNI CAGNNNCTG 3 cut(s) 605, 926, 1268
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 4 cut(s) 52, 966, 1048, 1316
ApoI RAATTY 3 cut(s) 1019, 1192, 1237
AspLEI GCGC 1 cut(s) 744
AspS9I GGNCC 3 cut(s) 647, 1058, 1316
AsuC2I CCSGG 2 cut(s) 224, 338
AsuHPI GGTGA 4 cut(s) 169, 212, 548, 767
AsuNHI GCTAGC 1 cut(s) 362
AvaII GGWCC 2 cut(s) 647, 1058
BaeGI GKGCMC 1 cut(s) 338
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 2 cut(s) 134, 1110
BccI CCATC 5 cut(s) 63, 103, 638, 752, 1351
BcgI CGANNNNNNTGC 2 cut(s) 1391, 1425
BciT130I CCWGG 3 cut(s) 480, 581, 721
BciVI GTATCC 2 cut(s) 230, 586
BcnI CCSGG 2 cut(s) 224, 338
BcoDI GTCTC 1 cut(s) 629
BcuI ACTAGT 2 cut(s) 815, 1336
BfaI CTAG 6 cut(s) 303, 363, 641, 816, 1299, 1337
BfmI CTRYAG 2 cut(s) 915, 1201
BfrI CTTAAG 1 cut(s) 1430
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 2 cut(s) 230, 586
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 5 cut(s) 224, 338, 480, 581, 721
Bme18I GGWCC 2 cut(s) 647, 1058
BmgT120I GGNCC 3 cut(s) 647, 1058, 1316
BmrFI CCNGG 5 cut(s) 224, 338, 480, 581, 721
BmrI ACTGGG 1 cut(s) 1135
BmsI GCATC 6 cut(s) 917, 934, 1010, 1016, 1414, 1444
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 1135
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 2 cut(s) 134, 1110
Bpu1102I GCTNAGC 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 224, 338
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 383, 1174
BsaWI WCCGGW 2 cut(s) 400, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 974, 1004
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1301
Bse118I RCCGGY 1 cut(s) 1046
Bse1I ACTGG 5 cut(s) 411, 732, 774, 939, 1130
Bse3DI GCAATG 2 cut(s) 177, 954
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 3 cut(s) 480, 581, 721
BseDI CCNNGG 2 cut(s) 383, 1174
BseGI GGATG 2 cut(s) 488, 1381
BseLI CCNNNNNNNGG 2 cut(s) 485, 1301
BseMI GCAATG 2 cut(s) 177, 954
BseMII CTCAG 4 cut(s) 125, 384, 1101, 1245
BseNI ACTGG 5 cut(s) 411, 732, 774, 939, 1130
BseRI GAGGAG 2 cut(s) 73, 1194
BseSI GKGCMC 1 cut(s) 338
BsgI GTGCAG 2 cut(s) 942, 1209
Bsh1236I CGCG 2 cut(s) 652, 1377
BshFI GGCC 4 cut(s) 54, 968, 1050, 1318
BsiHKAI GWGCWC 1 cut(s) 392
BsiSI CCGG 6 cut(s) 223, 338, 401, 1047, 1061, 1319
BslFI GGGAC 1 cut(s) 1311
BslI CCNNNNNNNGG 2 cut(s) 485, 1301
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 1311
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 4 cut(s) 54, 968, 1050, 1318
Bsp1286I GDGCHC 2 cut(s) 338, 392
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 343, 1093
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 1174
BspACI CCGC 8 cut(s) 15, 101, 113, 650, 837, 953, 969, 1130
BspANI GGCC 4 cut(s) 54, 968, 1050, 1318
BspCNI CTCAG 4 cut(s) 126, 385, 1102, 1244
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 2 cut(s) 652, 1377
BspHI TCATGA 2 cut(s) 42, 555
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 1101
BspTI CTTAAG 1 cut(s) 1430
BsrDI GCAATG 2 cut(s) 177, 954
BsrFI RCCGGY 1 cut(s) 1046
BsrI ACTGG 5 cut(s) 411, 732, 774, 939, 1130
BssAI RCCGGY 1 cut(s) 1046
BssECI CCNNGG 2 cut(s) 383, 1174
BssMI GATC 2 cut(s) 343, 1093
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 383, 1174
Bst2UI CCWGG 3 cut(s) 480, 581, 721
Bst4CI ACNGT 2 cut(s) 7, 1354
Bst6I CTCTTC 3 cut(s) 439, 627, 1269
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 1430
BstAPI GCANNNNNTGC 3 cut(s) 914, 926, 935
BstC8I GCNNGC 3 cut(s) 364, 677, 1417
BstDEI CTNAG 6 cut(s) 134, 321, 393, 416, 1110, 1231
BstDSI CCRYGG 1 cut(s) 1174
BstF5I GGATG 2 cut(s) 488, 1381
BstFNI CGCG 2 cut(s) 652, 1377
BstHHI GCGC 1 cut(s) 744
BstKTI GATC 2 cut(s) 346, 1096
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 2 cut(s) 343, 1093
BstNI CCWGG 3 cut(s) 480, 581, 721
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 5 cut(s) 222, 336, 478, 579, 719
BstSFI CTRYAG 2 cut(s) 915, 1201
BstSLI GKGCMC 1 cut(s) 338
BstUI CGCG 2 cut(s) 652, 1377
BstV2I GAAGAC 1 cut(s) 210
BsuI GTATCC 2 cut(s) 230, 586
BsuRI GGCC 4 cut(s) 54, 968, 1050, 1318
BtgI CCRYGG 1 cut(s) 1174
BtgZI GCGATG 2 cut(s) 301, 1358
BtsCI GGATG 2 cut(s) 488, 1381
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 3 cut(s) 210, 946, 1123
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 3 cut(s) 364, 677, 1417
CaiI CAGNNNCTG 3 cut(s) 605, 926, 1268
CciI TCATGA 2 cut(s) 42, 555
CfoI GCGC 1 cut(s) 744
Cfr10I RCCGGY 1 cut(s) 1046
Cfr13I GGNCC 3 cut(s) 647, 1058, 1316
CseI GACGC 3 cut(s) 215, 1274, 1313
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 2 cut(s) 654, 1340
CviAII CATG 5 cut(s) 43, 233, 556, 1090, 1175
CviQI GTAC 2 cut(s) 654, 1340
DdeI CTNAG 6 cut(s) 134, 321, 393, 416, 1110, 1231
DpnI GATC 2 cut(s) 345, 1095
DpnII GATC 2 cut(s) 343, 1093
DraI TTTAAA 1 cut(s) 1024
DrdI GACNNNNNNGTC 1 cut(s) 1065
DseDI GACNNNNNNGTC 1 cut(s) 1065
EaeI YGGCCR 2 cut(s) 966, 1048
Eam1104I CTCTTC 3 cut(s) 439, 627, 1269
EarI CTCTTC 3 cut(s) 439, 627, 1269
EciI GGCGGA 1 cut(s) 1145
Eco130I CCWWGG 2 cut(s) 383, 1174
Eco47I GGWCC 2 cut(s) 647, 1058
Eco57I CTGAAG 1 cut(s) 176
EcoRII CCWGG 3 cut(s) 478, 579, 719
EcoT14I CCWWGG 2 cut(s) 383, 1174
ErhI CCWWGG 2 cut(s) 383, 1174
FaeI CATG 5 cut(s) 46, 236, 559, 1093, 1178
FaqI GGGAC 1 cut(s) 1311
FatI CATG 5 cut(s) 42, 232, 555, 1089, 1174
FokI GGATG 2 cut(s) 495, 1368
FspBI CTAG 6 cut(s) 303, 363, 641, 816, 1299, 1337
FspI TGCGCA 1 cut(s) 743
GlaI GCGC 1 cut(s) 743
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 4 cut(s) 54, 968, 1050, 1318
HapII CCGG 6 cut(s) 223, 338, 401, 1047, 1061, 1319
HgaI GACGC 3 cut(s) 215, 1274, 1313
HhaI GCGC 1 cut(s) 744
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 5 cut(s) 46, 236, 559, 1093, 1178
Hin6I GCGC 1 cut(s) 742
HinP1I GCGC 1 cut(s) 742
HincII GTYRAC 1 cut(s) 1171
HindII GTYRAC 1 cut(s) 1171
HinfI GANTC 9 cut(s) 397, 493, 602, 701, 902, 989, 1079, 1259, 1364
HpaI GTTAAC 1 cut(s) 1171
HpaII CCGG 6 cut(s) 223, 338, 401, 1047, 1061, 1319
HphI GGTGA 4 cut(s) 169, 212, 548, 767
Hpy166II GTNNAC 1 cut(s) 1171
Hpy188I TCNGA 7 cut(s) 68, 195, 394, 607, 715, 1234, 1264
Hpy188III TCNNGA 7 cut(s) 43, 303, 401, 556, 629, 1076, 1347
Hpy8I GTNNAC 1 cut(s) 1171
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 3 cut(s) 347, 553, 565
HpyCH4III ACNGT 2 cut(s) 7, 1354
HpyCH4IV ACGT 1 cut(s) 1167
HpyF3I CTNAG 6 cut(s) 134, 321, 393, 416, 1110, 1231
HpySE526I ACGT 1 cut(s) 1167
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 5 cut(s) 46, 236, 559, 1093, 1178
HspAI GCGC 1 cut(s) 742
Kpn2I TCCGGA 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 1171
Kzo9I GATC 2 cut(s) 343, 1093
LmnI GCTCC 1 cut(s) 1321
LweI GCATC 6 cut(s) 917, 934, 1010, 1016, 1414, 1444
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 6 cut(s) 303, 363, 641, 816, 1299, 1337
MaeII ACGT 1 cut(s) 1167
MaeIII GTNAC 2 cut(s) 173, 682
MalI GATC 2 cut(s) 345, 1095
MboI GATC 2 cut(s) 343, 1093
MhlI GDGCHC 2 cut(s) 338, 392
MluCI AATT 8 cut(s) 294, 893, 1019, 1039, 1192, 1237, 1306, 1388
MlyI GAGTC 5 cut(s) 710, 896, 983, 1073, 1358
MroI TCCGGA 1 cut(s) 400
MseI TTAA 5 cut(s) 293, 368, 1023, 1170, 1431
MslI CAYNNNNRTG 2 cut(s) 385, 1101
MspA1I CMGCKG 3 cut(s) 101, 914, 926
MspCI CTTAAG 1 cut(s) 1430
MspI CCGG 6 cut(s) 223, 338, 401, 1047, 1061, 1319
MspR9I CCNGG 5 cut(s) 224, 338, 480, 581, 721
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 3 cut(s) 480, 581, 721
MvnI CGCG 2 cut(s) 652, 1377
NciI CCSGG 2 cut(s) 224, 338
NcoI CCATGG 1 cut(s) 1174
NdeII GATC 2 cut(s) 343, 1093
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 5 cut(s) 46, 236, 559, 1093, 1178
NmeAIII GCCGAG 2 cut(s) 30, 884
NmuCI GTSAC 2 cut(s) 173, 682
NsbI TGCGCA 1 cut(s) 743
NspI RCATGY 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 385
PagI TCATGA 2 cut(s) 42, 555
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 397, 493, 602, 1259
PleI GAGTC 5 cut(s) 709, 896, 983, 1073, 1358
PpsI GAGTC 5 cut(s) 709, 896, 983, 1073, 1358
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 478, 579, 719
PspGI CCWGG 3 cut(s) 478, 579, 719
PspPI GGNCC 3 cut(s) 647, 1058, 1316
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 3 cut(s) 605, 926, 1268
PvuII CAGCTG 2 cut(s) 914, 926
RsaI GTAC 2 cut(s) 655, 1341
RsaNI GTAC 2 cut(s) 654, 1340
RseI CAYNNNNRTG 2 cut(s) 385, 1101
SaqAI TTAA 5 cut(s) 293, 368, 1023, 1170, 1431
Sau3AI GATC 2 cut(s) 343, 1093
Sau96I GGNCC 3 cut(s) 647, 1058, 1316
SchI GAGTC 5 cut(s) 710, 896, 983, 1073, 1358
ScrFI CCNGG 5 cut(s) 224, 338, 480, 581, 721
SduI GDGCHC 2 cut(s) 338, 392
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 6 cut(s) 917, 934, 1010, 1016, 1414, 1444
SfcI CTRYAG 2 cut(s) 915, 1201
SinI GGWCC 2 cut(s) 647, 1058
SmiMI CAYNNNNRTG 2 cut(s) 385, 1101
SmlI CTYRAG 1 cut(s) 1430
SmoI CTYRAG 1 cut(s) 1430
SpeI ACTAGT 2 cut(s) 815, 1336
Sse9I AATT 8 cut(s) 294, 893, 1019, 1039, 1192, 1237, 1306, 1388
SsiI CCGC 8 cut(s) 15, 101, 113, 650, 837, 953, 969, 1130
SspMI CTAG 6 cut(s) 303, 363, 641, 816, 1299, 1337
StyD4I CCNGG 5 cut(s) 222, 336, 478, 579, 719
StyI CCWWGG 2 cut(s) 383, 1174
TaaI ACNGT 2 cut(s) 7, 1354
TaiI ACGT 1 cut(s) 1170
TaqI TCGA 1 cut(s) 1034
TasI AATT 8 cut(s) 294, 893, 1019, 1039, 1192, 1237, 1306, 1388
TauI GCSGC 3 cut(s) 104, 956, 971
TfiI GAWTC 4 cut(s) 397, 493, 602, 1259
Tru1I TTAA 5 cut(s) 293, 368, 1023, 1170, 1431
Tru9I TTAA 5 cut(s) 293, 368, 1023, 1170, 1431
TscAI CASTG 3 cut(s) 217, 946, 1130
TseFI GTSAC 2 cut(s) 173, 682
Tsp45I GTSAC 2 cut(s) 173, 682
TspDTI ATGAA 9 cut(s) 59, 262, 572, 1032, 1119, 1272, 1287, 1361, 1370
TspRI CASTG 3 cut(s) 217, 946, 1130
Vha464I CTTAAG 1 cut(s) 1430
VpaK11BI GGWCC 2 cut(s) 647, 1058
XapI RAATTY 3 cut(s) 1019, 1192, 1237
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XspI CTAG 6 cut(s) 303, 363, 641, 816, 1299, 1337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.