pycom08g13420

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003928
Physical Location & Seq
Forward (+)
12199340 .. 12200462
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g13420.1

Sequence Viewer

Length: 876 bp
ATGGGGAAAACAACTTTTCTTAGGCACTTGGCTGATTCTCAGGACATCGATATTGTATCTCTCAAGAAAATAAAAGAACACTTCCTATTGAGTTTGATAGTTTCTTTACGATTGATACGTGAGGGCAGATTTCAATCTCTTGACCAGTTTCTGCCTCGTGAATATTGTATATCTCTTGCTGACAATATAGAAAGTCAAGTCAAGTTGAGGGAGGAAGGCTATTTTCAACTCTTTTCTGAACTGCATAAATTTGCGGATTCTGATGAACTTAAGCTTAAGTTCGATGAAGGGCCGACAATGAGCAAGCTTTGGATTGTGAATTTGGGTGGCAGTGAGAGTCTAGCAAAAACTGAAGTTCAAGGGGACAGCCTGAAGGAAGCTCAAAACATCAATAAATCTCTTTCAGCTCTTAGGGATATGATTTCAGCTTTGTTAAGAATTAAGGCTTTGAACTGGTTCGGTCATATGGATGAAGAGAATGCCAAGGCCATATCCCTGTTTGAGAATGTAGTGGGCCTTAGTGATGCTATTGAAGATGCAGTCAAGTGGGTAGAGGATCTGATTAGAAGAATAATTACTGGAAATATATTTGATCTTGGCTTTGCACAGCTTGCAAAAGAGTTCTCAAATGAGGGTGCATTAGACCATGGAAGAATGGTGTACTCTACCTGCTCTATAAATTCACTTGAAAATGGAGCTGTGGTTGCTGAGATTTTGCGAAGGGGGCATTTTCTTATTGCACTACAAGGTGGAATTGATAAGCTTACGTGTGATGCATTGGCTAAAGCTATCAAGCAAATACTCTATTTTCAGTACAAGGACGACAAGCGAAAGGCTGCTTTTAAGAATCTATTTTTCCGAAGGAACTACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

292

Amino Acids

33.11

Weight (kDa)

6.41

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 98 - 146 1.5e-11 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 677
AciI CCGC 1 cut(s) 254
AclWI GGATC 1 cut(s) 564
AcsI RAATTY 3 cut(s) 248, 319, 679
AcuI CTGAAG 2 cut(s) 372, 392
AfaI GTAC 2 cut(s) 662, 815
AflII CTTAAG 2 cut(s) 269, 275
AflIII ACRYGT 1 cut(s) 767
AgsI TTSAA 6 cut(s) 134, 227, 359, 451, 533, 689
AluBI AGCT 9 cut(s) 274, 307, 380, 407, 428, 610, 698, 763, 788
AluI AGCT 9 cut(s) 274, 307, 380, 407, 428, 610, 698, 763, 788
AlwI GGATC 1 cut(s) 564
AlwNI CAGNNNCTG 1 cut(s) 151
AoxI GGCC 3 cut(s) 290, 486, 514
ApeKI GCWGC 1 cut(s) 836
ApoI RAATTY 3 cut(s) 248, 319, 679
Asp700I GAANNNNTTC 1 cut(s) 455
AspS9I GGNCC 2 cut(s) 290, 514
BarI GAAGNNNNNNTAC 2 cut(s) 559, 591
BauI CACGAG 1 cut(s) 156
BbvI GCAGC 1 cut(s) 823
BfaI CTAG 1 cut(s) 341
BfrI CTTAAG 2 cut(s) 269, 275
BfuAI ACCTGC 1 cut(s) 677
BisI GCNGC 1 cut(s) 837
BlsI GCNGC 1 cut(s) 838
BmgT120I GGNCC 2 cut(s) 290, 514
BmsI GCATC 3 cut(s) 514, 526, 763
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 48
BsaAI YACGTR 2 cut(s) 119, 768
BsaBI GATNNNNATC 1 cut(s) 133
BsaJI CCNNGG 2 cut(s) 483, 646
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bse1I ACTGG 3 cut(s) 145, 458, 583
Bse8I GATNNNNATC 1 cut(s) 133
BseCI ATCGAT 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 483, 646
BseGI GGATG 1 cut(s) 475
BseJI GATNNNNATC 1 cut(s) 133
BseMII CTCAG 2 cut(s) 53, 699
BseNI ACTGG 3 cut(s) 145, 458, 583
BseXI GCAGC 1 cut(s) 823
BshFI GGCC 3 cut(s) 292, 488, 516
BshVI ATCGAT 1 cut(s) 48
BslFI GGGAC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 484
BsnI GGCC 3 cut(s) 292, 488, 516
Bsp143I GATC 2 cut(s) 556, 592
Bsp19I CCATGG 1 cut(s) 646
BspACI CCGC 1 cut(s) 254
BspANI GGCC 3 cut(s) 292, 488, 516
BspCNI CTCAG 2 cut(s) 52, 700
BspDI ATCGAT 1 cut(s) 48
BspMI ACCTGC 1 cut(s) 677
BspPI GGATC 1 cut(s) 564
BspTI CTTAAG 2 cut(s) 269, 275
BsrI ACTGG 3 cut(s) 145, 458, 583
BssECI CCNNGG 2 cut(s) 483, 646
BssMI GATC 2 cut(s) 556, 592
BssSI CACGAG 1 cut(s) 156
BssT1I CCWWGG 2 cut(s) 483, 646
Bst2BI CACGAG 1 cut(s) 156
Bst6I CTCTTC 1 cut(s) 468
BstAFI CTTAAG 2 cut(s) 269, 275
BstAPI GCANNNNNTGC 1 cut(s) 611
BstBAI YACGTR 2 cut(s) 119, 768
BstC8I GCNNGC 2 cut(s) 305, 612
BstDEI CTNAG 5 cut(s) 20, 39, 410, 518, 708
BstDSI CCRYGG 1 cut(s) 646
BstF5I GGATG 1 cut(s) 475
BstKTI GATC 2 cut(s) 559, 595
BstMBI GATC 2 cut(s) 556, 592
BstMWI GCNNNNNNNGC 3 cut(s) 611, 704, 724
BstV1I GCAGC 1 cut(s) 823
BstX2I RGATCY 1 cut(s) 556
BstYI RGATCY 1 cut(s) 556
Bsu15I ATCGAT 1 cut(s) 48
BsuRI GGCC 3 cut(s) 292, 488, 516
BsuTUI ATCGAT 1 cut(s) 48
BtgI CCRYGG 1 cut(s) 646
BtsCI GGATG 1 cut(s) 475
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 337
BveI ACCTGC 1 cut(s) 677
Cac8I GCNNGC 2 cut(s) 305, 612
CaiI CAGNNNCTG 1 cut(s) 151
Cfr13I GGNCC 2 cut(s) 290, 514
ClaI ATCGAT 1 cut(s) 48
Csp6I GTAC 2 cut(s) 661, 814
CviAII CATG 1 cut(s) 647
CviQI GTAC 2 cut(s) 661, 814
DdeI CTNAG 5 cut(s) 20, 39, 410, 518, 708
DpnI GATC 2 cut(s) 558, 594
DpnII GATC 2 cut(s) 556, 592
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco130I CCWWGG 2 cut(s) 483, 646
Eco57I CTGAAG 2 cut(s) 372, 392
EcoT14I CCWWGG 2 cut(s) 483, 646
EcoT22I ATGCAT 1 cut(s) 778
ErhI CCWWGG 2 cut(s) 483, 646
FaeI CATG 1 cut(s) 650
FaqI GGGAC 1 cut(s) 377
FatI CATG 1 cut(s) 646
FauNDI CATATG 1 cut(s) 465
Fnu4HI GCNGC 1 cut(s) 837
FokI GGATG 1 cut(s) 482
Fsp4HI GCNGC 1 cut(s) 837
FspBI CTAG 1 cut(s) 341
GluI GCNGC 1 cut(s) 837
HaeIII GGCC 3 cut(s) 292, 488, 516
Hin1II CATG 1 cut(s) 650
HindIII AAGCTT 3 cut(s) 272, 305, 761
HinfI GANTC 4 cut(s) 35, 257, 337, 847
Hpy166II GTNNAC 1 cut(s) 661
Hpy188I TCNGA 4 cut(s) 238, 262, 561, 860
Hpy188III TCNNGA 4 cut(s) 41, 64, 140, 158
Hpy8I GTNNAC 1 cut(s) 661
HpyAV CCTTC 5 cut(s) 209, 281, 367, 714, 855
HpyCH4IV ACGT 2 cut(s) 118, 767
HpyCH4V TGCA 7 cut(s) 244, 539, 605, 614, 638, 740, 776
HpyF10VI GCNNNNNNNGC 3 cut(s) 611, 704, 724
HpyF3I CTNAG 5 cut(s) 20, 39, 410, 518, 708
HpySE526I ACGT 2 cut(s) 118, 767
Hsp92II CATG 1 cut(s) 650
Kzo9I GATC 2 cut(s) 556, 592
LmnI GCTCC 1 cut(s) 695
LpnPI CCDG 7 cut(s) 26, 158, 383, 439, 509, 564, 682
Lsp1109I GCAGC 1 cut(s) 823
LweI GCATC 3 cut(s) 514, 526, 763
MaeI CTAG 1 cut(s) 341
MaeII ACGT 2 cut(s) 118, 767
MalI GATC 2 cut(s) 558, 594
MboI GATC 2 cut(s) 556, 592
MboII GAAGA 4 cut(s) 485, 545, 579, 663
MflI RGATCY 1 cut(s) 556
MluCI AATT 6 cut(s) 248, 319, 438, 573, 679, 753
MlyI GAGTC 1 cut(s) 346
MnlI CCTC 6 cut(s) 115, 165, 201, 205, 547, 625
Mph1103I ATGCAT 1 cut(s) 778
MroXI GAANNNNTTC 1 cut(s) 455
MseI TTAA 5 cut(s) 270, 276, 434, 441, 843
MslI CAYNNNNRTG 1 cut(s) 468
MspCI CTTAAG 2 cut(s) 269, 275
Mva1269I GAATGC 1 cut(s) 484
MwoI GCNNNNNNNGC 3 cut(s) 611, 704, 724
NcoI CCATGG 1 cut(s) 646
NdeI CATATG 1 cut(s) 465
NdeII GATC 2 cut(s) 556, 592
NlaIII CATG 1 cut(s) 650
NsiI ATGCAT 1 cut(s) 778
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PctI GAATGC 1 cut(s) 484
PdmI GAANNNNTTC 1 cut(s) 455
PfeI GAWTC 3 cut(s) 35, 257, 847
PkrI GCNGC 1 cut(s) 838
PleI GAGTC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 345
Ppu21I YACGTR 2 cut(s) 119, 768
PspPI GGNCC 2 cut(s) 290, 514
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 556
RsaI GTAC 2 cut(s) 662, 815
RsaNI GTAC 2 cut(s) 661, 814
RseI CAYNNNNRTG 1 cut(s) 468
SaqAI TTAA 5 cut(s) 270, 276, 434, 441, 843
SatI GCNGC 1 cut(s) 837
Sau3AI GATC 2 cut(s) 556, 592
Sau96I GGNCC 2 cut(s) 290, 514
SchI GAGTC 1 cut(s) 346
SfaNI GCATC 3 cut(s) 514, 526, 763
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 3 cut(s) 62, 269, 275
SmoI CTYRAG 3 cut(s) 62, 269, 275
Sse9I AATT 6 cut(s) 248, 319, 438, 573, 679, 753
SsiI CCGC 1 cut(s) 254
SspI AATATT 1 cut(s) 164
SspMI CTAG 1 cut(s) 341
StyI CCWWGG 2 cut(s) 483, 646
TaiI ACGT 2 cut(s) 121, 770
TaqI TCGA 2 cut(s) 48, 282
TaqII GACCGA 1 cut(s) 449
TasI AATT 6 cut(s) 248, 319, 438, 573, 679, 753
TatI WGTACW 2 cut(s) 660, 813
TfiI GAWTC 3 cut(s) 35, 257, 847
Tru1I TTAA 5 cut(s) 270, 276, 434, 441, 843
Tru9I TTAA 5 cut(s) 270, 276, 434, 441, 843
TscAI CASTG 1 cut(s) 337
TseI GCWGC 1 cut(s) 836
TspDTI ATGAA 3 cut(s) 279, 300, 486
TspRI CASTG 1 cut(s) 337
Vha464I CTTAAG 2 cut(s) 269, 275
XapI RAATTY 3 cut(s) 248, 319, 679
XmnI GAANNNNTTC 1 cut(s) 455
XspI CTAG 1 cut(s) 341
Zsp2I ATGCAT 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.