Rh2CG120200

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003928
Physical Location & Seq
Forward (+)
10651133 .. 10651971
839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG120200.1

Sequence Viewer

Length: 432 bp
ATGACAGAGGTGAAGCAGATGAGGCATGACATCGCTTCACTCCTTCAGAAAGGTCAAGAAGTCACTGCTCGTATCTTGATTGAACATTTGACAAGAGACCAGAATTCTTTGGCTGCCTATGAACTCATTAGGCTCTTCTGCCAATTGGTCGTGGAAAGACTTTTAATCATCGAAAAGCAAAGGCAATGTCCGCCTGATTTGAAAGAAGGGATTGCTAGTTTGATCTTTGCAGCCCCAAGGTGCTGCGAGATTCCAGAACTAATGACAGTCAGGAATATTTTTGAGAAGAAATATGGAAGAGATTTTGTATCTGCAGCGAGTGATCTAAGACCTGAGTGTGGAGTGAATGGAAAGATGATTGAGAATCTCTCTATCAAAACTCCCAGTGGTGAAGTGAAGTTGAAACTCATGAAGGAAATAGCCAATGAGTAA

Protein Analysis

143

Amino Acids

16.27

Weight (kDa)

7.62

Isoelectric Point (pI)

41.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 3 - 143 3e-49 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 191
AcsI RAATTY 1 cut(s) 103
AcuI CTGAAG 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 338
AgsI TTSAA 3 cut(s) 83, 202, 403
Alw26I GTCTC 1 cut(s) 90
ApeKI GCWGC 4 cut(s) 113, 230, 243, 314
ApoI RAATTY 1 cut(s) 103
ArsI GACNNNNNNTTYG 2 cut(s) 172, 204
AsuHPI GGTGA 2 cut(s) 22, 401
BbvI GCAGC 4 cut(s) 100, 230, 242, 326
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 4 cut(s) 114, 231, 244, 315
BlsI GCNGC 4 cut(s) 115, 232, 245, 316
BmrI ACTGGG 1 cut(s) 378
BmuI ACTGGG 1 cut(s) 378
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 236
Bsc4I CCNNNNNNNGG 1 cut(s) 338
Bse1I ACTGG 1 cut(s) 384
Bse3DI GCAATG 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 236
BseLI CCNNNNNNNGG 1 cut(s) 338
BseMI GCAATG 1 cut(s) 191
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 384
BseXI GCAGC 4 cut(s) 100, 230, 242, 326
BslI CCNNNNNNNGG 1 cut(s) 338
BsmAI GTCTC 1 cut(s) 90
Bso31I GGTCTC 1 cut(s) 90
Bsp143I GATC 2 cut(s) 222, 322
BspACI CCGC 1 cut(s) 191
BspCNI CTCAG 1 cut(s) 325
BspHI TCATGA 1 cut(s) 408
BspMAI CTGCAG 1 cut(s) 316
BspQI GCTCTTC 1 cut(s) 140
BspTNI GGTCTC 1 cut(s) 90
BsrDI GCAATG 1 cut(s) 191
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 2 cut(s) 222, 322
BssT1I CCWWGG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 2 cut(s) 140, 292
BstDEI CTNAG 2 cut(s) 326, 333
BstKTI GATC 2 cut(s) 225, 325
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 2 cut(s) 222, 322
BstMWI GCNNNNNNNGC 2 cut(s) 22, 190
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 100, 230, 242, 326
BtgZI GCGATG 1 cut(s) 16
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 2 cut(s) 63, 391
CciI TCATGA 1 cut(s) 408
CviAII CATG 2 cut(s) 26, 409
CviJI RGCY 4 cut(s) 113, 133, 233, 422
CviKI_1 RGCY 4 cut(s) 113, 133, 233, 422
DdeI CTNAG 2 cut(s) 326, 333
DpnI GATC 2 cut(s) 224, 324
DpnII GATC 2 cut(s) 222, 322
Eam1104I CTCTTC 2 cut(s) 140, 292
EarI CTCTTC 2 cut(s) 140, 292
EciI GGCGGA 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 236
Eco31I GGTCTC 1 cut(s) 90
Eco57I CTGAAG 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 236
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 2 cut(s) 29, 412
FaiI YATR 4 cut(s) 27, 120, 294, 410
FatI CATG 2 cut(s) 25, 408
Fnu4HI GCNGC 4 cut(s) 114, 231, 244, 315
Fsp4HI GCNGC 4 cut(s) 114, 231, 244, 315
FspBI CTAG 1 cut(s) 216
GluI GCNGC 4 cut(s) 114, 231, 244, 315
Hin1II CATG 2 cut(s) 29, 412
HinfI GANTC 2 cut(s) 250, 364
HphI GGTGA 2 cut(s) 22, 401
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 5 cut(s) 56, 76, 254, 271, 409
HpyAV CCTTC 3 cut(s) 53, 200, 406
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4V TGCA 2 cut(s) 230, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 190
HpyF3I CTNAG 2 cut(s) 326, 333
Hsp92II CATG 2 cut(s) 29, 412
Kzo9I GATC 2 cut(s) 222, 322
LguI GCTCTTC 1 cut(s) 140
LpnPI CCDG 6 cut(s) 113, 207, 256, 267, 345, 397
Lsp1109I GCAGC 4 cut(s) 100, 230, 242, 326
MaeI CTAG 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 61
MalI GATC 2 cut(s) 224, 324
MboI GATC 2 cut(s) 222, 322
MboII GAAGA 3 cut(s) 127, 298, 309
MfeI CAATTG 1 cut(s) 143
MluCI AATT 2 cut(s) 103, 143
MnlI CCTC 1 cut(s) 15
MseI TTAA 1 cut(s) 164
MunI CAATTG 1 cut(s) 143
MwoI GCNNNNNNNGC 2 cut(s) 22, 190
NdeII GATC 2 cut(s) 222, 322
NlaIII CATG 2 cut(s) 29, 412
NmuCI GTSAC 1 cut(s) 61
PagI TCATGA 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 140
PfeI GAWTC 2 cut(s) 250, 364
PkrI GCNGC 4 cut(s) 115, 232, 245, 316
PstI CTGCAG 1 cut(s) 316
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 1 cut(s) 164
SatI GCNGC 4 cut(s) 114, 231, 244, 315
Sau3AI GATC 2 cut(s) 222, 322
SetI ASST 4 cut(s) 12, 55, 242, 334
SfcI CTRYAG 1 cut(s) 312
Sse9I AATT 2 cut(s) 103, 143
SsiI CCGC 1 cut(s) 191
SspI AATATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 216
StyI CCWWGG 1 cut(s) 236
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 1 cut(s) 171
TasI AATT 2 cut(s) 103, 143
TfiI GAWTC 2 cut(s) 250, 364
Tru1I TTAA 1 cut(s) 164
Tru9I TTAA 1 cut(s) 164
TscAI CASTG 2 cut(s) 70, 391
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 4 cut(s) 113, 230, 243, 314
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 2 cut(s) 135, 425
TspRI CASTG 2 cut(s) 70, 391
XapI RAATTY 1 cut(s) 103
XspI CTAG 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.