Rh2BG118800

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003928
Physical Location & Seq
Forward (+)
10092505 .. 10095983
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG118800.1

Sequence Viewer

Length: 1467 bp
ATGACCGTCGCCACCGCGGCCTCAACCCAAACCAAAAAGCTCATGAAGCTCGGCCTCTCTCTTTTCCGATGGGGCTTCAACTCCTCCAAATGCAAAACAGCGGCGAAGATGGCTGTGGCGAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATTGCTTCTCTGCTTCAGAAAGGTGAAGACGCCACTGCTCGTATCCGGGTTGAACATGTGATTAGAGAACAGAATGTTTTGGCTGCCAATGAGTTCATTGAGCTGTTCTGTGAGTTAATCGTGTCTAGACTTCAAATCATCGCTAAGCAAAGGGAGTGCCCGGCTGATCTCAAAGAAGGGATTGCTAGCTTAATCTTTGCAACACCAAGGTGCTCTGAGATTCCGGAACTGGTGGCACTAAGGAATATCTTTGAGAAGAAATATGGAAGAGATTTCGTAGCTGCAGCTACTGATTTGCGACCTGGATGTGGTGTGAATCGCATTCTCATTGACAAGCTGTCTGTTAGAACCCCTACGGGTGAAGTAAAGCTGAAAATCATGAAGGAAATAGCGAAGGAATACCAGGTTGATTGGGATACAACAGAATCTGAACAAGAACTTCTCAAACCTCCAGAAGAGACTCTAGATGGACCGCGTACTTTTGTTAGTGCTACCAGCTTGCCTGTGACAACCCAATCTTTTGAGTCTAACAAGTCAGAAACCAGGCTTTCTTCCAGTTCGTTGTGCAGAACAAGTGGTGGTGGACAGAGGGAAACCAGTTCTACCGGCAGAATGAGTGATGGTGGAGAAAGGGAAACTAGTTCCTTTCGCAGAACAAGCGGTGGTGGAGTAAGAGAAACCAGTGTTTCTTCTGGCTCTTTTCACAGAATAAGTGGTGGTGGAGAAAGGGGACATTTGCAATTTGCAGACTCGGCATCAGCTGCCGCAGCAGCTGCAAAATCTGCCAGTGATGCAATGGCGGCTGCACAAGTGGCTGCATTTCTGGCCAACAAAGACTCCAATCGGGCATCTGCTAATGAATTTAAAACCCTTTCGAGCAATTCCACCGGCCAACAAGGACCAGATAGTCCATCTCTTGACTCTTATGACATGGATCATCAATATGAAGCTGAGGGAAAAACACACTGGGCGGAGAGTTTGGACAGGTCATATTCTTCAAACAATGACGTTAACCATGGAGGAGAGGGGAAGAATTTTGTCTATAGAAGATACAGCTACAATGCTGCACCATCAGAAAATTCAGGTATCAAGTTTGATGAATCAGACACCGATGAAGAGATTGAAATGGAAGCACCTCCTAGTGGGAATTATCAAGGGCCCGAGCGTCCTCCACCACCAGTACCTTCGGCAACTGTTAGGCAGGACTCACTTCATCGCGTTCATCCCAAATTGCCTGATTACGATACACTTGCTGCTCGCTTTGATGCCCTTAAGCATCATCACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

488

Amino Acids

53.51

Weight (kDa)

8.1

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 2.2e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
AasI GACNNNNNNGTC 1 cut(s) 1083
Acc36I ACCTGC 1 cut(s) 139
AccII CGCG 3 cut(s) 17, 652, 1395
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 8 cut(s) 15, 17, 101, 650, 837, 942, 977, 1148
AclWI GGATC 1 cut(s) 1119
AcoI YGGCCR 2 cut(s) 1002, 1066
AcsI RAATTY 3 cut(s) 1037, 1210, 1255
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 207
AfaI GTAC 2 cut(s) 655, 1359
AfiI CCNNNNNNNGG 2 cut(s) 485, 1319
AflII CTTAAG 1 cut(s) 1448
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 79, 230, 311, 1176, 1301
AhdI GACNNNNNGTC 1 cut(s) 514
AhlI ACTAGT 1 cut(s) 815
AjnI CCWGG 3 cut(s) 478, 579, 719
AleI CACNNNNGTG 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 1 cut(s) 629
AlwI GGATC 1 cut(s) 1119
AlwNI CAGNNNCTG 3 cut(s) 467, 605, 950
Ama87I CYCGRG 1 cut(s) 1337
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 5 cut(s) 18, 52, 1002, 1066, 1334
ApaI GGGCCC 1 cut(s) 1338
ApoI RAATTY 3 cut(s) 1037, 1210, 1255
AspS9I GGNCC 4 cut(s) 647, 1076, 1334, 1335
AsuC2I CCSGG 2 cut(s) 224, 338
AsuHPI GGTGA 3 cut(s) 169, 212, 548
AsuNHI GCTAGC 1 cut(s) 362
AvaI CYCGRG 1 cut(s) 1337
AvaII GGWCC 2 cut(s) 647, 1076
BaeGI GKGCMC 2 cut(s) 338, 1338
BalI TGGCCA 1 cut(s) 1004
BanII GRGCYC 1 cut(s) 1338
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 392
BbvCI CCTCAGC 2 cut(s) 134, 1128
BccI CCATC 6 cut(s) 63, 103, 638, 791, 1096, 1255
BcgI CGANNNNNNTGC 2 cut(s) 1409, 1443
BciT130I CCWGG 3 cut(s) 480, 581, 721
BciVI GTATCC 2 cut(s) 230, 586
BcnI CCSGG 2 cut(s) 224, 338
BcoDI GTCTC 1 cut(s) 629
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 5 cut(s) 303, 363, 641, 816, 1317
BfmI CTRYAG 2 cut(s) 459, 1219
BfrI CTTAAG 1 cut(s) 1448
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 2 cut(s) 230, 586
BglI GCCNNNNNGGC 1 cut(s) 17
BlpI GCTNAGC 1 cut(s) 321
Bme1390I CCNGG 5 cut(s) 224, 338, 480, 581, 721
Bme18I GGWCC 2 cut(s) 647, 1076
BmeRI GACNNNNNGTC 1 cut(s) 514
BmeT110I CYCGRG 1 cut(s) 1337
BmgT120I GGNCC 4 cut(s) 647, 1076, 1334, 1335
BmiI GGNNCC 1 cut(s) 1336
BmrFI CCNGG 5 cut(s) 224, 338, 480, 581, 721
BmrI ACTGGG 1 cut(s) 1153
BmsI GCATC 5 cut(s) 941, 958, 1034, 1432, 1462
BmtI GCTAGC 1 cut(s) 366
BmuI ACTGGG 1 cut(s) 1153
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 2 cut(s) 134, 1128
Bpu1102I GCTNAGC 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 224, 338
BsaHI GRCGYC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 15, 383, 1192
BsaWI WCCGGW 1 cut(s) 400
BsaXI ACNNNNNCTCC 2 cut(s) 998, 1028
Bsc4I CCNNNNNNNGG 2 cut(s) 485, 1319
Bse118I RCCGGY 2 cut(s) 782, 1064
Bse1I ACTGG 7 cut(s) 411, 732, 774, 858, 963, 1148, 1355
Bse3DI GCAATG 2 cut(s) 177, 978
BseAI TCCGGA 1 cut(s) 400
BseBI CCWGG 3 cut(s) 480, 581, 721
BseDI CCNNGG 3 cut(s) 15, 383, 1192
BseGI GGATG 2 cut(s) 488, 1399
BseLI CCNNNNNNNGG 2 cut(s) 485, 1319
BseMI GCAATG 2 cut(s) 177, 978
BseMII CTCAG 3 cut(s) 125, 384, 1119
BseNI ACTGG 7 cut(s) 411, 732, 774, 858, 963, 1148, 1355
BseRI GAGGAG 2 cut(s) 73, 1212
BseSI GKGCMC 2 cut(s) 338, 1338
BsgI GTGCAG 3 cut(s) 763, 966, 1227
Bsh1236I CGCG 3 cut(s) 17, 652, 1395
BshFI GGCC 5 cut(s) 20, 54, 1004, 1068, 1336
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 1 cut(s) 1337
BsiSI CCGG 5 cut(s) 223, 338, 401, 783, 1065
BslFI GGGAC 1 cut(s) 921
BslI CCNNNNNNNGG 2 cut(s) 485, 1319
BsmAI GTCTC 1 cut(s) 629
BsmFI GGGAC 1 cut(s) 921
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 5 cut(s) 20, 54, 1004, 1068, 1336
BsoBI CYCGRG 1 cut(s) 1337
Bsp120I GGGCCC 1 cut(s) 1334
Bsp1286I GDGCHC 3 cut(s) 338, 392, 1338
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 2 cut(s) 343, 1111
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 1 cut(s) 1192
BspACI CCGC 8 cut(s) 15, 17, 101, 650, 837, 942, 977, 1148
BspANI GGCC 5 cut(s) 20, 54, 1004, 1068, 1336
BspCNI CTCAG 3 cut(s) 126, 385, 1120
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 3 cut(s) 17, 652, 1395
BspHI TCATGA 2 cut(s) 42, 555
BspLI GGNNCC 1 cut(s) 1336
BspMAI CTGCAG 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 366
BspPI GGATC 1 cut(s) 1119
BspTI CTTAAG 1 cut(s) 1448
BsrDI GCAATG 2 cut(s) 177, 978
BsrFI RCCGGY 2 cut(s) 782, 1064
BsrI ACTGG 7 cut(s) 411, 732, 774, 858, 963, 1148, 1355
BssAI RCCGGY 2 cut(s) 782, 1064
BssECI CCNNGG 3 cut(s) 15, 383, 1192
BssMI GATC 2 cut(s) 343, 1111
BssNI GRCGYC 1 cut(s) 207
BssT1I CCWWGG 2 cut(s) 383, 1192
Bst2UI CCWGG 3 cut(s) 480, 581, 721
Bst4CI ACNGT 2 cut(s) 7, 1372
Bst6I CTCTTC 3 cut(s) 439, 627, 1287
BstACI GRCGYC 1 cut(s) 207
BstAFI CTTAAG 1 cut(s) 1448
BstAPI GCANNNNNTGC 3 cut(s) 938, 950, 959
BstC8I GCNNGC 3 cut(s) 364, 677, 1435
BstDEI CTNAG 5 cut(s) 134, 321, 393, 416, 1128
BstDSI CCRYGG 2 cut(s) 15, 1192
BstF5I GGATG 2 cut(s) 488, 1399
BstFNI CGCG 3 cut(s) 17, 652, 1395
BstKTI GATC 2 cut(s) 346, 1114
BstMAI GTCTC 1 cut(s) 629
BstMBI GATC 2 cut(s) 343, 1111
BstNI CCWGG 3 cut(s) 480, 581, 721
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 5 cut(s) 222, 336, 478, 579, 719
BstSFI CTRYAG 2 cut(s) 459, 1219
BstSLI GKGCMC 2 cut(s) 338, 1338
BstUI CGCG 3 cut(s) 17, 652, 1395
BstV2I GAAGAC 1 cut(s) 210
BsuI GTATCC 2 cut(s) 230, 586
BsuRI GGCC 5 cut(s) 20, 54, 1004, 1068, 1336
BtgI CCRYGG 2 cut(s) 15, 1192
BtgZI GCGATG 2 cut(s) 301, 1376
BtsCI GGATG 2 cut(s) 488, 1399
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 4 cut(s) 210, 865, 970, 1141
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 3 cut(s) 364, 677, 1435
CaiI CAGNNNCTG 3 cut(s) 467, 605, 950
CciI TCATGA 2 cut(s) 42, 555
Cfr10I RCCGGY 2 cut(s) 782, 1064
Cfr13I GGNCC 4 cut(s) 647, 1076, 1334, 1335
Cfr42I CCGCGG 1 cut(s) 18
CseI GACGC 2 cut(s) 215, 1331
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 2 cut(s) 654, 1358
CviAII CATG 5 cut(s) 43, 233, 556, 1108, 1193
CviQI GTAC 2 cut(s) 654, 1358
DdeI CTNAG 5 cut(s) 134, 321, 393, 416, 1128
DpnI GATC 2 cut(s) 345, 1113
DpnII GATC 2 cut(s) 343, 1111
DraI TTTAAA 1 cut(s) 1042
DrdI GACNNNNNNGTC 1 cut(s) 1083
DriI GACNNNNNGTC 1 cut(s) 514
DseDI GACNNNNNNGTC 1 cut(s) 1083
EaeI YGGCCR 2 cut(s) 1002, 1066
Eam1104I CTCTTC 3 cut(s) 439, 627, 1287
Eam1105I GACNNNNNGTC 1 cut(s) 514
EarI CTCTTC 3 cut(s) 439, 627, 1287
EciI GGCGGA 1 cut(s) 1163
Eco130I CCWWGG 2 cut(s) 383, 1192
Eco24I GRGCYC 1 cut(s) 1338
Eco47I GGWCC 2 cut(s) 647, 1076
Eco57I CTGAAG 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 1337
EcoO109I RGGNCCY 1 cut(s) 1334
EcoRII CCWGG 3 cut(s) 478, 579, 719
EcoT14I CCWWGG 2 cut(s) 383, 1192
EcoT38I GRGCYC 1 cut(s) 1338
ErhI CCWWGG 2 cut(s) 383, 1192
FaeI CATG 5 cut(s) 46, 236, 559, 1111, 1196
FaqI GGGAC 1 cut(s) 921
FatI CATG 5 cut(s) 42, 232, 555, 1107, 1192
FokI GGATG 2 cut(s) 495, 1386
FriOI GRGCYC 1 cut(s) 1338
FspBI CTAG 5 cut(s) 303, 363, 641, 816, 1317
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 5 cut(s) 20, 54, 1004, 1068, 1336
HapII CCGG 5 cut(s) 223, 338, 401, 783, 1065
HgaI GACGC 2 cut(s) 215, 1331
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 5 cut(s) 46, 236, 559, 1111, 1196
HincII GTYRAC 1 cut(s) 1189
HindII GTYRAC 1 cut(s) 1189
HpaI GTTAAC 1 cut(s) 1189
HpaII CCGG 5 cut(s) 223, 338, 401, 783, 1065
HphI GGTGA 3 cut(s) 169, 212, 548
Hpy166II GTNNAC 2 cut(s) 761, 1189
Hpy188I TCNGA 7 cut(s) 68, 195, 394, 607, 715, 1252, 1282
Hpy188III TCNNGA 7 cut(s) 43, 303, 401, 556, 629, 641, 1094
Hpy8I GTNNAC 2 cut(s) 761, 1189
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 4 cut(s) 347, 553, 565, 1371
HpyCH4III ACNGT 2 cut(s) 7, 1372
HpyCH4IV ACGT 1 cut(s) 1185
HpyF3I CTNAG 5 cut(s) 134, 321, 393, 416, 1128
HpySE526I ACGT 1 cut(s) 1185
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 5 cut(s) 46, 236, 559, 1111, 1196
Kpn2I TCCGGA 1 cut(s) 400
KspAI GTTAAC 1 cut(s) 1189
KspI CCGCGG 1 cut(s) 18
Kzo9I GATC 2 cut(s) 343, 1111
LweI GCATC 5 cut(s) 941, 958, 1034, 1432, 1462
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 5 cut(s) 303, 363, 641, 816, 1317
MaeII ACGT 1 cut(s) 1185
MaeIII GTNAC 2 cut(s) 173, 682
MalI GATC 2 cut(s) 345, 1113
MboI GATC 2 cut(s) 343, 1111
MhlI GDGCHC 3 cut(s) 338, 392, 1338
MlsI TGGCCA 1 cut(s) 1004
MluCI AATT 7 cut(s) 917, 1037, 1057, 1210, 1255, 1324, 1406
MluNI TGGCCA 1 cut(s) 1004
MlyI GAGTC 6 cut(s) 631, 710, 920, 1007, 1091, 1376
Mox20I TGGCCA 1 cut(s) 1004
MroI TCCGGA 1 cut(s) 400
MscI TGGCCA 1 cut(s) 1004
MseI TTAA 5 cut(s) 293, 368, 1041, 1188, 1449
MslI CAYNNNNRTG 2 cut(s) 385, 1119
Msp20I TGGCCA 1 cut(s) 1004
MspA1I CMGCKG 4 cut(s) 17, 101, 938, 950
MspCI CTTAAG 1 cut(s) 1448
MspI CCGG 5 cut(s) 223, 338, 401, 783, 1065
MspR9I CCNGG 5 cut(s) 224, 338, 480, 581, 721
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 3 cut(s) 480, 581, 721
MvnI CGCG 3 cut(s) 17, 652, 1395
NciI CCSGG 2 cut(s) 224, 338
NcoI CCATGG 1 cut(s) 1192
NdeII GATC 2 cut(s) 343, 1111
NheI GCTAGC 1 cut(s) 362
NlaIII CATG 5 cut(s) 46, 236, 559, 1111, 1196
NlaIV GGNNCC 1 cut(s) 1336
NmeAIII GCCGAG 2 cut(s) 30, 908
NmuCI GTSAC 2 cut(s) 173, 682
NspI RCATGY 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 385
PagI TCATGA 2 cut(s) 42, 555
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 4 cut(s) 397, 493, 602, 1277
PleI GAGTC 6 cut(s) 631, 709, 920, 1007, 1091, 1376
PpsI GAGTC 6 cut(s) 631, 709, 920, 1007, 1091, 1376
PscI ACATGT 1 cut(s) 232
Psp6I CCWGG 3 cut(s) 478, 579, 719
PspGI CCWGG 3 cut(s) 478, 579, 719
PspN4I GGNNCC 1 cut(s) 1336
PspOMI GGGCCC 1 cut(s) 1334
PspPI GGNCC 4 cut(s) 647, 1076, 1334, 1335
PstI CTGCAG 1 cut(s) 463
PstNI CAGNNNCTG 3 cut(s) 467, 605, 950
PvuII CAGCTG 2 cut(s) 938, 950
RsaI GTAC 2 cut(s) 655, 1359
RsaNI GTAC 2 cut(s) 654, 1358
RseI CAYNNNNRTG 2 cut(s) 385, 1119
SacII CCGCGG 1 cut(s) 18
SaqAI TTAA 5 cut(s) 293, 368, 1041, 1188, 1449
Sau3AI GATC 2 cut(s) 343, 1111
Sau96I GGNCC 4 cut(s) 647, 1076, 1334, 1335
SchI GAGTC 6 cut(s) 631, 710, 920, 1007, 1091, 1376
ScrFI CCNGG 5 cut(s) 224, 338, 480, 581, 721
SduI GDGCHC 3 cut(s) 338, 392, 1338
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 5 cut(s) 941, 958, 1034, 1432, 1462
SfcI CTRYAG 2 cut(s) 459, 1219
Sfr303I CCGCGG 1 cut(s) 18
SgrBI CCGCGG 1 cut(s) 18
SinI GGWCC 2 cut(s) 647, 1076
SmiMI CAYNNNNRTG 2 cut(s) 385, 1119
SmlI CTYRAG 1 cut(s) 1448
SmoI CTYRAG 1 cut(s) 1448
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 7 cut(s) 917, 1037, 1057, 1210, 1255, 1324, 1406
SsiI CCGC 8 cut(s) 15, 17, 101, 650, 837, 942, 977, 1148
SspMI CTAG 5 cut(s) 303, 363, 641, 816, 1317
StyD4I CCNGG 5 cut(s) 222, 336, 478, 579, 719
StyI CCWWGG 2 cut(s) 383, 1192
TaaI ACNGT 2 cut(s) 7, 1372
TaiI ACGT 1 cut(s) 1188
TaqI TCGA 1 cut(s) 1052
TasI AATT 7 cut(s) 917, 1037, 1057, 1210, 1255, 1324, 1406
TauI GCSGC 4 cut(s) 20, 104, 944, 980
TfiI GAWTC 4 cut(s) 397, 493, 602, 1277
Tru1I TTAA 5 cut(s) 293, 368, 1041, 1188, 1449
Tru9I TTAA 5 cut(s) 293, 368, 1041, 1188, 1449
TscAI CASTG 4 cut(s) 217, 865, 970, 1148
TseFI GTSAC 2 cut(s) 173, 682
Tsp45I GTSAC 2 cut(s) 173, 682
TspDTI ATGAA 9 cut(s) 59, 262, 572, 1050, 1137, 1290, 1305, 1379, 1388
TspRI CASTG 4 cut(s) 217, 865, 970, 1148
Vha464I CTTAAG 1 cut(s) 1448
VpaK11BI GGWCC 2 cut(s) 647, 1076
XapI RAATTY 3 cut(s) 1037, 1210, 1255
XbaI TCTAGA 2 cut(s) 302, 640
XceI RCATGY 1 cut(s) 236
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XspI CTAG 5 cut(s) 303, 363, 641, 816, 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.