MD15G1235100.v1.1

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003928
Physical Location & Seq
Forward (+)
19322117 .. 19325391
3275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1235100.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGACTGTCGCCACCGTAGCCACCACCCACACCAGGAAGCTGGTGAAGCTCAGCCGCTCTCTCTTCCGATGGGGATTCAACTCCTCCAAATGCAAAATGCAAGCGAAGATGGCGGTGGCTAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATCGCCTTGCTCCTACAATCTGGTCAAGATGCCACTGCTCGAATCCGGGTTGAACATGTGATCAGAGAACAGAACGTTTTGGCTGCCAATGAGTTCATTGAGCTCTTCTGTGAATTAGTGGTCTCTAGGCTTTCTATCATCGCAAAGCAAAGGGAGTGTCCGCCTGATCTCAAAGAAGGGATTGCAAGTTTAATCTTTGCCTCCCCCAGGTGTTCTGAGATTCCTGAACTGATCGCACTAAGGAATATTTTTGAGAAAAAGTATGGTAAAGATTTTGTATCTGCGGCTGTTGATGTACGTCCCAGCTGTGGTGTGAATCGCATGCTGATTGACAAGCTCTCTGTTAGAACCCCTACGGGCGAAGTAAAGTTGAAACTCCTGAAGGAGATAGCCAAGGAATACCAGGTTGAATGGGATACATCAGAATCTGAACAGGAGCTTCTCAAGCCTCCAGAAGAGCTTATACAAGGACCACGCAATTTTGTTAGTGCTACCAGCATGCCTGTGCAGAGTGTGCCGCCTCAATCTGTTGAGACTAATAAGCCAGACACTAGTTCTTCACGCAGGACAAGTGGTGGTGTAGAACGAAGAACAAGTGGTGGTGGAGAACAAAGAACAAGTGGTGGTGGAGAACGCAGAACAAGTAGTGGTGGTGAAAGAGGACATATGCAATTTGTAGACTCAGCATCAGCTGCTGAAGCAGCTGCAAAATCCGCCAGTGAGGCAATGGCTGCTGCACAAGTTGCTGCATATTTGGCCAACAAAGACTCCAATCAAGCAGCTCAATCATCTTCGAGCGTCAATACTGGATTCGGAACCCCTTCAGGAAATTCCACTGGCCGCTTTATGCCAGATTCTCCATTATTCGGGTCTCAGAATATGGTCAATAATGGATTTGGAACCCCTTCTGGCAATTCCACTGGCTTCTTTACGCCAGATTCTCCATCAGTTGGCTCTCAGAATAGCGATCACCAATCTAAAGCTCCTGGAGTTGATAGGTCTCACTCTTCAAGAGATGAGGAGGCGATGCATAGCCATGGAAATGAGAAGCAATTCATCTACAGGAGGTACAGCTACAATAATGCCCCATCAGTACATTCAGATATTAAGTTTGATGAATCGGACTGTGATGAAGAAGTTGAAATGGAGTCACCTCCCCGCGGCTTTCATCAAGCACCTGAGCGTCCTCCACCACAAGTACCTTCAGCTGCAAGGCAGGACTCAGTTCCTCGAGTTCATCCAAAGTTACCAGATTATGACGCACTTGCTGCTCGCTTTGACGCCCTCAAGTATCGGAAGTCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

495

Amino Acids

54.13

Weight (kDa)

9.07

Isoelectric Point (pI)

56.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 1.9e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 1127
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 855
AccII CGCG 1 cut(s) 1338
AciI CCGC 9 cut(s) 55, 113, 338, 461, 695, 891, 1018, 1336, 1338
AclI AACGTT 1 cut(s) 252
AcoI YGGCCR 2 cut(s) 933, 1015
AcsI RAATTY 1 cut(s) 1006
AcuI CTGAAG 4 cut(s) 578, 894, 984, 1365
AcyI GRCGYC 1 cut(s) 1458
AfaI GTAC 4 cut(s) 474, 1247, 1272, 1377
AfiI CCNNNNNNNGG 6 cut(s) 33, 384, 485, 1043, 1127, 1337
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 6 cut(s) 79, 230, 550, 587, 1188, 1319
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 4 cut(s) 32, 383, 579, 1162
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 4 cut(s) 304, 704, 1053, 1182
AlwNI CAGNNNCTG 2 cut(s) 605, 872
Ama87I CYCGRG 1 cut(s) 1407
AoxI GGCC 2 cut(s) 933, 1015
ApoI RAATTY 1 cut(s) 1006
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
Asp700I GAANNNNTTC 3 cut(s) 997, 1081, 1229
AspS9I GGNCC 1 cut(s) 647
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 5 cut(s) 55, 169, 842, 1139, 1320
AvaI CYCGRG 1 cut(s) 1407
AvaII GGWCC 1 cut(s) 647
BalI TGGCCA 1 cut(s) 935
BanII GRGCYC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 624, 656
Bbv12I GWGCWC 1 cut(s) 282
BbvCI CCTCAGC 1 cut(s) 134
BccI CCATC 4 cut(s) 63, 103, 1129, 1273
BciT130I CCWGG 4 cut(s) 34, 385, 581, 1164
BciVI GTATCC 1 cut(s) 586
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 4 cut(s) 304, 704, 1053, 1182
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 4 cut(s) 120, 303, 729, 1483
BfmI CTRYAG 1 cut(s) 1237
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 586
BglI GCCNNNNNGGC 1 cut(s) 899
BlpI GCTNAGC 1 cut(s) 50
Bme1390I CCNGG 5 cut(s) 34, 224, 385, 581, 1164
Bme18I GGWCC 1 cut(s) 647
BmeT110I CYCGRG 1 cut(s) 1407
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 2 cut(s) 994, 1078
BmrFI CCNGG 5 cut(s) 34, 224, 385, 581, 1164
BmsI GCATC 3 cut(s) 196, 872, 1194
BpmI CTGGAG 2 cut(s) 612, 1185
Bpu10I CCTNAGC 2 cut(s) 134, 1356
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 605, 1448
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 1458
BsaI GGTCTC 3 cut(s) 304, 1053, 1182
BsaJI CCNNGG 4 cut(s) 383, 570, 1213, 1336
BsaXI ACNNNNNCTCC 2 cut(s) 929, 959
Bsc4I CCNNNNNNNGG 6 cut(s) 33, 384, 485, 1043, 1127, 1337
Bse1I ACTGG 4 cut(s) 894, 988, 1018, 1102
Bse3DI GCAATG 1 cut(s) 909
BseBI CCWGG 4 cut(s) 34, 385, 581, 1164
BseDI CCNNGG 4 cut(s) 383, 570, 1213, 1336
BseGI GGATG 2 cut(s) 1414, 1478
BseLI CCNNNNNNNGG 6 cut(s) 33, 384, 485, 1043, 1127, 1337
BseMI GCAATG 1 cut(s) 909
BseMII CTCAG 8 cut(s) 64, 125, 384, 873, 1064, 1148, 1347, 1413
BseNI ACTGG 4 cut(s) 894, 988, 1018, 1102
BseRI GAGGAG 2 cut(s) 73, 1211
BseYI CCCAGC 1 cut(s) 479
BsgI GTGCAG 2 cut(s) 704, 897
Bsh1236I CGCG 1 cut(s) 1338
BshFI GGCC 2 cut(s) 935, 1017
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 1407
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 1 cut(s) 462
BslI CCNNNNNNNGG 6 cut(s) 33, 384, 485, 1043, 1127, 1337
BsmAI GTCTC 4 cut(s) 304, 704, 1053, 1182
BsmFI GGGAC 1 cut(s) 462
BsnI GGCC 2 cut(s) 935, 1017
Bso31I GGTCTC 3 cut(s) 304, 1053, 1182
BsoBI CYCGRG 1 cut(s) 1407
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 4 cut(s) 237, 343, 408, 1144
Bsp1720I GCTNAGC 1 cut(s) 50
Bsp19I CCATGG 1 cut(s) 1213
BspACI CCGC 9 cut(s) 55, 113, 338, 461, 695, 891, 1018, 1336, 1338
BspANI GGCC 2 cut(s) 935, 1017
BspCNI CTCAG 8 cut(s) 63, 126, 385, 872, 1063, 1147, 1348, 1412
BspFNI CGCG 1 cut(s) 1338
BspLI GGNNCC 2 cut(s) 994, 1078
BspMI ACCTGC 1 cut(s) 139
BspQI GCTCTTC 2 cut(s) 287, 627
BspTNI GGTCTC 3 cut(s) 304, 1053, 1182
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 909
BsrI ACTGG 4 cut(s) 894, 988, 1018, 1102
BssECI CCNNGG 4 cut(s) 383, 570, 1213, 1336
BssMI GATC 4 cut(s) 237, 343, 408, 1144
BssNI GRCGYC 1 cut(s) 1458
BssT1I CCWWGG 2 cut(s) 570, 1213
Bst2UI CCWGG 4 cut(s) 34, 385, 581, 1164
Bst4CI ACNGT 3 cut(s) 7, 16, 1304
Bst6I CTCTTC 4 cut(s) 68, 287, 627, 1189
BstACI GRCGYC 1 cut(s) 1458
BstAPI GCANNNNNTGC 5 cut(s) 691, 869, 908, 920, 1445
BstC8I GCNNGC 4 cut(s) 102, 500, 677, 1450
BstDEI CTNAG 9 cut(s) 50, 134, 393, 416, 859, 1050, 1134, 1356, 1399
BstDSI CCRYGG 2 cut(s) 1213, 1336
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 2 cut(s) 1414, 1478
BstFNI CGCG 1 cut(s) 1338
BstKTI GATC 4 cut(s) 240, 346, 411, 1147
BstMAI GTCTC 4 cut(s) 304, 704, 1053, 1182
BstMBI GATC 4 cut(s) 237, 343, 408, 1144
BstNI CCWGG 4 cut(s) 34, 385, 581, 1164
BstNSI RCATGY 3 cut(s) 236, 502, 679
BstSCI CCNGG 5 cut(s) 32, 222, 383, 579, 1162
BstSFI CTRYAG 1 cut(s) 1237
BstUI CGCG 1 cut(s) 1338
BstXI CCANNNNNNTGG 1 cut(s) 40
BsuI GTATCC 1 cut(s) 586
BsuRI GGCC 2 cut(s) 935, 1017
BtgI CCRYGG 2 cut(s) 1213, 1336
BtgZI GCGATG 3 cut(s) 163, 301, 1217
BtsCI GGATG 2 cut(s) 1414, 1478
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 4 cut(s) 210, 901, 1011, 1095
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 4 cut(s) 102, 500, 677, 1450
CaiI CAGNNNCTG 2 cut(s) 605, 872
Cfr13I GGNCC 1 cut(s) 647
Cfr42I CCGCGG 1 cut(s) 1339
CseI GACGC 4 cut(s) 964, 1349, 1445, 1466
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 4 cut(s) 473, 1246, 1271, 1376
CviAII CATG 4 cut(s) 233, 499, 676, 1214
CviQI GTAC 4 cut(s) 473, 1246, 1271, 1376
DdeI CTNAG 9 cut(s) 50, 134, 393, 416, 859, 1050, 1134, 1356, 1399
DpnI GATC 4 cut(s) 239, 345, 410, 1146
DpnII GATC 4 cut(s) 237, 343, 408, 1144
EaeI YGGCCR 2 cut(s) 933, 1015
Eam1104I CTCTTC 4 cut(s) 68, 287, 627, 1189
EarI CTCTTC 4 cut(s) 68, 287, 627, 1189
EciI GGCGGA 2 cut(s) 327, 880
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 2 cut(s) 570, 1213
Eco24I GRGCYC 1 cut(s) 282
Eco31I GGTCTC 3 cut(s) 304, 1053, 1182
Eco47I GGWCC 1 cut(s) 647
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 4 cut(s) 578, 894, 984, 1365
Eco88I CYCGRG 1 cut(s) 1407
EcoICRI GAGCTC 1 cut(s) 280
EcoNI CCTNNNNNAGG 1 cut(s) 382
EcoRII CCWGG 4 cut(s) 32, 383, 579, 1162
EcoT14I CCWWGG 2 cut(s) 570, 1213
EcoT22I ATGCAT 1 cut(s) 1209
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 570, 1213
FaeI CATG 4 cut(s) 236, 502, 679, 1217
FaqI GGGAC 1 cut(s) 462
FatI CATG 4 cut(s) 232, 498, 675, 1213
FauI CCCGC 1 cut(s) 1343
FauNDI CATATG 1 cut(s) 843
FbaI TGATCA 1 cut(s) 237
FblI GTMKAC 1 cut(s) 855
FokI GGATG 2 cut(s) 1401, 1465
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 4 cut(s) 120, 303, 729, 1483
GsaI CCCAGC 1 cut(s) 483
GsuI CTGGAG 2 cut(s) 612, 1185
HaeIII GGCC 2 cut(s) 935, 1017
HapII CCGG 1 cut(s) 223
HgaI GACGC 4 cut(s) 964, 1349, 1445, 1466
Hin1I GRCGYC 1 cut(s) 1458
Hin1II CATG 4 cut(s) 236, 502, 679, 1217
HpaII CCGG 1 cut(s) 223
HphI GGTGA 5 cut(s) 55, 169, 842, 1139, 1320
Hpy166II GTNNAC 1 cut(s) 856
Hpy188III TCNNGA 6 cut(s) 203, 401, 556, 629, 1002, 1188
Hpy8I GTNNAC 1 cut(s) 856
HpyAV CCTTC 5 cut(s) 347, 553, 1008, 1092, 1389
HpyCH4III ACNGT 3 cut(s) 7, 16, 1304
HpyCH4IV ACGT 2 cut(s) 252, 475
HpyF3I CTNAG 9 cut(s) 50, 134, 393, 416, 859, 1050, 1134, 1356, 1399
HpySE526I ACGT 2 cut(s) 252, 475
Hsp92I GRCGYC 1 cut(s) 1458
Hsp92II CATG 4 cut(s) 236, 502, 679, 1217
Ksp22I TGATCA 1 cut(s) 237
KspI CCGCGG 1 cut(s) 1339
Kzo9I GATC 4 cut(s) 237, 343, 408, 1144
LguI GCTCTTC 2 cut(s) 287, 627
LmnI GCTCC 3 cut(s) 192, 613, 1165
LweI GCATC 3 cut(s) 196, 872, 1194
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 4 cut(s) 120, 303, 729, 1483
MaeII ACGT 2 cut(s) 252, 475
MaeIII GTNAC 3 cut(s) 173, 1326, 1422
MalI GATC 4 cut(s) 239, 345, 410, 1146
MbiI CCGCTC 1 cut(s) 57
MboI GATC 4 cut(s) 237, 343, 408, 1144
MboII GAAGA 9 cut(s) 55, 118, 274, 644, 726, 777, 960, 1176, 1322
MhlI GDGCHC 1 cut(s) 282
MlsI TGGCCA 1 cut(s) 935
MluCI AATT 6 cut(s) 290, 655, 848, 1006, 1090, 1229
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 4 cut(s) 851, 938, 1334, 1391
Mox20I TGGCCA 1 cut(s) 935
Mph1103I ATGCAT 1 cut(s) 1209
MroXI GAANNNNTTC 3 cut(s) 997, 1081, 1229
MscI TGGCCA 1 cut(s) 935
MseI TTAA 2 cut(s) 368, 1284
MslI CAYNNNNRTG 3 cut(s) 680, 1212, 1218
Msp20I TGGCCA 1 cut(s) 935
MspA1I CMGCKG 5 cut(s) 483, 869, 881, 1338, 1385
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 5 cut(s) 34, 224, 385, 581, 1164
MvaI CCWGG 4 cut(s) 34, 385, 581, 1164
MvnI CGCG 1 cut(s) 1338
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 1213
NdeI CATATG 1 cut(s) 843
NdeII GATC 4 cut(s) 237, 343, 408, 1144
NlaIII CATG 4 cut(s) 236, 502, 679, 1217
NlaIV GGNNCC 2 cut(s) 994, 1078
NmuCI GTSAC 2 cut(s) 173, 1326
NsiI ATGCAT 1 cut(s) 1209
NspI RCATGY 3 cut(s) 236, 502, 679
PaeI GCATGC 2 cut(s) 502, 679
PaeR7I CTCGAG 1 cut(s) 1407
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PciSI GCTCTTC 2 cut(s) 287, 627
PdmI GAANNNNTTC 3 cut(s) 997, 1081, 1229
PfeI GAWTC 9 cut(s) 75, 219, 397, 493, 602, 987, 1031, 1115, 1295
PflMI CCANNNNNTGG 1 cut(s) 1127
PfoI TCCNGGA 1 cut(s) 1162
PleI GAGTC 4 cut(s) 851, 938, 1333, 1391
PpsI GAGTC 4 cut(s) 851, 938, 1333, 1391
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 252
Psp6I CCWGG 4 cut(s) 32, 383, 579, 1162
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 4 cut(s) 32, 383, 579, 1162
PspN4I GGNNCC 2 cut(s) 994, 1078
PspPI GGNCC 1 cut(s) 647
PspXI VCTCGAGB 1 cut(s) 1407
PstNI CAGNNNCTG 2 cut(s) 605, 872
PvuII CAGCTG 4 cut(s) 483, 869, 881, 1385
RsaI GTAC 4 cut(s) 474, 1247, 1272, 1377
RsaNI GTAC 4 cut(s) 473, 1246, 1271, 1376
RseI CAYNNNNRTG 3 cut(s) 680, 1212, 1218
SacI GAGCTC 1 cut(s) 282
SacII CCGCGG 1 cut(s) 1339
SapI GCTCTTC 2 cut(s) 287, 627
SaqAI TTAA 2 cut(s) 368, 1284
Sau3AI GATC 4 cut(s) 237, 343, 408, 1144
Sau96I GGNCC 1 cut(s) 647
SchI GAGTC 4 cut(s) 851, 938, 1334, 1391
ScrFI CCNGG 5 cut(s) 34, 224, 385, 581, 1164
SduI GDGCHC 1 cut(s) 282
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 3 cut(s) 196, 872, 1194
SfcI CTRYAG 1 cut(s) 1237
Sfr274I CTCGAG 1 cut(s) 1407
Sfr303I CCGCGG 1 cut(s) 1339
SgrBI CCGCGG 1 cut(s) 1339
SinI GGWCC 1 cut(s) 647
SlaI CTCGAG 1 cut(s) 1407
SmiMI CAYNNNNRTG 3 cut(s) 680, 1212, 1218
SmlI CTYRAG 3 cut(s) 620, 1407, 1463
SmoI CTYRAG 3 cut(s) 620, 1407, 1463
SpeI ACTAGT 1 cut(s) 728
SphI GCATGC 2 cut(s) 502, 679
Sse9I AATT 6 cut(s) 290, 655, 848, 1006, 1090, 1229
SsiI CCGC 9 cut(s) 55, 113, 338, 461, 695, 891, 1018, 1336, 1338
SspI AATATT 1 cut(s) 424
SspMI CTAG 4 cut(s) 120, 303, 729, 1483
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 5 cut(s) 32, 222, 383, 579, 1162
StyI CCWWGG 2 cut(s) 570, 1213
TaaI ACNGT 3 cut(s) 7, 16, 1304
TaiI ACGT 2 cut(s) 255, 478
TaqI TCGA 3 cut(s) 217, 971, 1408
TasI AATT 6 cut(s) 290, 655, 848, 1006, 1090, 1229
TatI WGTACW 1 cut(s) 1270
TauI GCSGC 5 cut(s) 57, 464, 697, 1020, 1341
TfiI GAWTC 9 cut(s) 75, 219, 397, 493, 602, 987, 1031, 1115, 1295
Tru1I TTAA 2 cut(s) 368, 1284
Tru9I TTAA 2 cut(s) 368, 1284
TscAI CASTG 4 cut(s) 217, 901, 1018, 1102
TseFI GTSAC 2 cut(s) 173, 1326
Tsp45I GTSAC 2 cut(s) 173, 1326
TspDTI ATGAA 6 cut(s) 262, 1222, 1308, 1323, 1334, 1403
TspRI CASTG 4 cut(s) 217, 901, 1018, 1102
Van91I CCANNNNNTGG 1 cut(s) 1127
VpaK11BI GGWCC 1 cut(s) 647
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 1 cut(s) 1006
XceI RCATGY 3 cut(s) 236, 502, 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 901
XhoI CTCGAG 1 cut(s) 1407
XmiI GTMKAC 1 cut(s) 855
XmnI GAANNNNTTC 3 cut(s) 997, 1081, 1229
XspI CTAG 4 cut(s) 120, 303, 729, 1483
Zsp2I ATGCAT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.