MD02G1121300.v1.1

IST1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003928
Physical Location & Seq
Forward (+)
9894503 .. 9901698
7196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1121300.v1.1.491

Sequence Viewer

Length: 1485 bp
ATGACGGTCGCCACCGCAGCCACCACCCACACCAAGAAGCTGGTGAAGCTCGGCCGCTCTCTCTTCCGATGGGGATTCAACTCCTCCAAATGCAAAATGCAAGCGAAGATGGCGGTGGCTAGAATAAAGCTGCTGAGGAACAAGAGGCAGGTGGTGGTGAAGCAGATGAGGCGTGACATCGCCTTACTCCTACAATCTGGTCAAGACGCCACTGCTCGAATCCGGGTTGAACATGTGATCAGAGAACAGAACATTTTGGCTGCCAATGAATTCATTGAGCTCTTCTGTGAATTAGTAGTATCTAGACTTTCTATCATCGCAAAGCAAAGGGAGTGTCCGCCTGATCTCAAAGAAGGGATTGCTAGTTTAATCTTTGCCTCCCCCAGGTGCTCTGAGATTCCTGAACTAATTGCCCTAAGGAATATTTTTGAGAAAAAGTATGGTAAAGATTTTGTATATGCGGCTGTTGATGTACGTCCCAGCTGTGGTGTGAATCGCATGCTGATTGACAAGCTCTCTGTTAGAACCCCTACGGGTGAAGTAAAGTTGAAACTCCTGAAGGAGATAGCCAAGGAATACCAGGTTGAATGGGATACATCGGAATCTGAACAGGAACTTCTCAAGCCCCCAGAAGAGCTTATACAAGGACACCGCAATTTTGTTAGTGCTACCAGCATGCCTGTGCACAGTGTGCAGCATCAATCTGTTGAGACTAATAAGCCAGACACTAGTTCTTCACGCAGAACAAGTGGTGGTGGAGAACGTAGAACAAGTGGTGGTGGAGAACGCAGAACATCAAGTGCTGGTGGTGATCGCAGATCAAGTAGTGGTGGTGAAAGGGGACAAATGCACTTTGTAGACTCCGCATCAGCTGCTGAAGCAGCCGCAAAATCTGCCAGTGATGCAATGGCTGCTGCACAAGTTGCTGCATATTTGGCCAACAAAGACTGCAATCAAGCAGCTCAATCATCTTCGAGCATCAATAATGGATTCAGAACCCCTTCAGGAAATTCCACTGGCCACTTTATGCCAGATTCTCCACCAGTCGCCTCTCAGAATATGGTCAATAATGGATTTGGAACCCCTTCTGGCAATTCCACTGGCTTCTTTACGCCAGATTCTTCATCAGTTGGCTCTCAGAATATCGATCACCAATCTAAAGCTCGTGGAGTCGATAGGTCTCACTCTTCAAGAGATGAAACAATGGATAGCCATGGACATGAGAAGAAGTTCATCTACAGGAGGTACAGCTACAATAATGCCCCATCAGAACAGTCGGATACTAAGTTTGATGACTCGGACTGCGATGAAGAAGTTGAAATGGAGTCACCTCCCAACAGCTTTCATCAAGCATCTGAGCGTACTCCACCACCAGTACCTTCAGCTGCAAGGCAAGACTCGACTCCTCGAGTTCATCCCAAGTTACCAGATTATGACGCACTTGCTGCACGCTTTGATGCCCTCAAGAATCGGAAGTCATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

495

Amino Acids

54.25

Weight (kDa)

9.09

Isoelectric Point (pI)

58.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 32 - 196 5.1e-61 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 57
AccI GTMKAC 1 cut(s) 858
AciI CCGC 8 cut(s) 15, 55, 113, 338, 461, 652, 864, 885
AcoI YGGCCR 3 cut(s) 52, 936, 1018
AcsI RAATTY 2 cut(s) 269, 1009
AcuI CTGAAG 4 cut(s) 578, 897, 987, 1365
AcyI GRCGYC 1 cut(s) 207
AdeI CACNNNGTG 1 cut(s) 691
AfaI GTAC 4 cut(s) 474, 1247, 1363, 1377
AfiI CCNNNNNNNGG 2 cut(s) 384, 485
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 6 cut(s) 79, 230, 550, 587, 1191, 1319
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 383, 579
Alw21I GWGCWC 3 cut(s) 282, 392, 687
Alw26I GTCTC 2 cut(s) 704, 1185
Alw44I GTGCAC 1 cut(s) 683
AlwNI CAGNNNCTG 1 cut(s) 875
Ama87I CYCGRG 1 cut(s) 1407
AoxI GGCC 3 cut(s) 52, 936, 1018
ApaLI GTGCAC 1 cut(s) 683
ApoI RAATTY 2 cut(s) 269, 1009
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
Asp700I GAANNNNTTC 3 cut(s) 1000, 1084, 1229
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 7 cut(s) 55, 169, 548, 821, 845, 1142, 1320
AvaI CYCGRG 1 cut(s) 1407
AxyI CCTNAGG 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 687
BalI TGGCCA 2 cut(s) 938, 1020
BanII GRGCYC 1 cut(s) 282
BarI GAAGNNNNNNTAC 4 cut(s) 624, 656, 1220, 1252
BauI CACGAG 1 cut(s) 1164
Bbv12I GWGCWC 3 cut(s) 282, 392, 687
BbvCI CCTCAGC 1 cut(s) 134
BccI CCATC 3 cut(s) 63, 103, 1273
BciT130I CCWGG 2 cut(s) 385, 581
BciVI GTATCC 2 cut(s) 586, 1273
BclI TGATCA 1 cut(s) 237
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 704, 1185
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 4 cut(s) 120, 303, 363, 729
BfmI CTRYAG 1 cut(s) 1237
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 2 cut(s) 586, 1273
Bme1390I CCNGG 3 cut(s) 224, 385, 581
BmeT110I CYCGRG 1 cut(s) 1407
BmiI GGNNCC 1 cut(s) 1081
BmrFI CCNGG 3 cut(s) 224, 385, 581
BmsI GCATC 6 cut(s) 706, 875, 892, 987, 1361, 1447
Bpu10I CCTNAGC 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 605, 1448
BpuMI CCSGG 1 cut(s) 224
Bsa29I ATCGAT 1 cut(s) 1146
BsaHI GRCGYC 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 1185
BsaJI CCNNGG 3 cut(s) 383, 570, 1213
Bsc4I CCNNNNNNNGG 2 cut(s) 384, 485
Bse1I ACTGG 5 cut(s) 897, 1021, 1043, 1105, 1373
Bse21I CCTNAGG 1 cut(s) 416
Bse3DI GCAATG 1 cut(s) 912
BseBI CCWGG 2 cut(s) 385, 581
BseCI ATCGAT 1 cut(s) 1146
BseDI CCNNGG 3 cut(s) 383, 570, 1213
BseGI GGATG 2 cut(s) 1414, 1478
BseLI CCNNNNNNNGG 2 cut(s) 384, 485
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 5 cut(s) 125, 384, 1067, 1151, 1347
BseNI ACTGG 5 cut(s) 897, 1021, 1043, 1105, 1373
BseRI GAGGAG 2 cut(s) 73, 1395
BseSI GKGCMC 1 cut(s) 687
BseX3I CGGCCG 1 cut(s) 52
BseYI CCCAGC 1 cut(s) 479
BsgI GTGCAG 3 cut(s) 713, 900, 1431
Bsh1285I CGRYCG 2 cut(s) 9, 55
BshFI GGCC 3 cut(s) 54, 938, 1020
BshVI ATCGAT 1 cut(s) 1146
BsiEI CGRYCG 2 cut(s) 9, 55
BsiHKAI GWGCWC 3 cut(s) 282, 392, 687
BsiHKCI CYCGRG 1 cut(s) 1407
BsiSI CCGG 1 cut(s) 223
BslFI GGGAC 2 cut(s) 462, 855
BslI CCNNNNNNNGG 2 cut(s) 384, 485
BsmAI GTCTC 2 cut(s) 704, 1185
BsmFI GGGAC 2 cut(s) 462, 855
BsnI GGCC 3 cut(s) 54, 938, 1020
Bso31I GGTCTC 1 cut(s) 1185
BsoBI CYCGRG 1 cut(s) 1407
Bsp1286I GDGCHC 3 cut(s) 282, 392, 687
Bsp143I GATC 5 cut(s) 237, 343, 811, 818, 1147
Bsp19I CCATGG 1 cut(s) 1213
BspACI CCGC 8 cut(s) 15, 55, 113, 338, 461, 652, 864, 885
BspANI GGCC 3 cut(s) 54, 938, 1020
BspCNI CTCAG 5 cut(s) 126, 385, 1066, 1150, 1348
BspDI ATCGAT 1 cut(s) 1146
BspLI GGNNCC 1 cut(s) 1081
BspMI ACCTGC 1 cut(s) 139
BspQI GCTCTTC 2 cut(s) 287, 627
BspTNI GGTCTC 1 cut(s) 1185
BsrBI CCGCTC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 5 cut(s) 897, 1021, 1043, 1105, 1373
BssECI CCNNGG 3 cut(s) 383, 570, 1213
BssMI GATC 5 cut(s) 237, 343, 811, 818, 1147
BssNI GRCGYC 1 cut(s) 207
BssSI CACGAG 1 cut(s) 1164
BssT1I CCWWGG 2 cut(s) 570, 1213
Bst2BI CACGAG 1 cut(s) 1164
Bst2UI CCWGG 2 cut(s) 385, 581
Bst4CI ACNGT 3 cut(s) 7, 689, 1275
Bst6I CTCTTC 4 cut(s) 68, 287, 627, 1192
BstACI GRCGYC 1 cut(s) 207
BstAPI GCANNNNNTGC 6 cut(s) 691, 872, 893, 911, 923, 1445
BstC8I GCNNGC 4 cut(s) 102, 500, 677, 1450
BstDEI CTNAG 7 cut(s) 134, 393, 416, 1053, 1137, 1284, 1356
BstDSI CCRYGG 1 cut(s) 1213
BstENI CCTNNNNNAGG 1 cut(s) 382
BstF5I GGATG 2 cut(s) 1414, 1478
BstKTI GATC 5 cut(s) 240, 346, 814, 821, 1150
BstMAI GTCTC 2 cut(s) 704, 1185
BstMBI GATC 5 cut(s) 237, 343, 811, 818, 1147
BstMCI CGRYCG 2 cut(s) 9, 55
BstNI CCWGG 2 cut(s) 385, 581
BstNSI RCATGY 3 cut(s) 236, 502, 679
BstSCI CCNGG 3 cut(s) 222, 383, 579
BstSFI CTRYAG 1 cut(s) 1237
BstSLI GKGCMC 1 cut(s) 687
BstXI CCANNNNNNTGG 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 52
Bsu15I ATCGAT 1 cut(s) 1146
Bsu36I CCTNAGG 1 cut(s) 416
BsuI GTATCC 2 cut(s) 586, 1273
BsuRI GGCC 3 cut(s) 54, 938, 1020
BsuTUI ATCGAT 1 cut(s) 1146
BtgI CCRYGG 1 cut(s) 1213
BtgZI GCGATG 3 cut(s) 163, 301, 1320
BtsCI GGATG 2 cut(s) 1414, 1478
BtsI GCAGTG 1 cut(s) 210
BtsIMutI CAGTG 5 cut(s) 210, 694, 904, 1014, 1098
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 4 cut(s) 102, 500, 677, 1450
CaiI CAGNNNCTG 1 cut(s) 875
ClaI ATCGAT 1 cut(s) 1146
CseI GACGC 2 cut(s) 215, 1445
CsiI ACCWGGT 1 cut(s) 579
Csp6I GTAC 4 cut(s) 473, 1246, 1362, 1376
CviAII CATG 5 cut(s) 233, 499, 676, 1214, 1220
CviQI GTAC 4 cut(s) 473, 1246, 1362, 1376
DdeI CTNAG 7 cut(s) 134, 393, 416, 1053, 1137, 1284, 1356
DpnI GATC 5 cut(s) 239, 345, 813, 820, 1149
DpnII GATC 5 cut(s) 237, 343, 811, 818, 1147
DraIII CACNNNGTG 1 cut(s) 691
EaeI YGGCCR 3 cut(s) 52, 936, 1018
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 4 cut(s) 68, 287, 627, 1192
EarI CTCTTC 4 cut(s) 68, 287, 627, 1192
EciI GGCGGA 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 280
EclXI CGGCCG 1 cut(s) 52
Eco130I CCWWGG 2 cut(s) 570, 1213
Eco24I GRGCYC 1 cut(s) 282
Eco31I GGTCTC 1 cut(s) 1185
Eco52I CGGCCG 1 cut(s) 52
Eco53kI GAGCTC 1 cut(s) 280
Eco57I CTGAAG 4 cut(s) 578, 897, 987, 1365
Eco81I CCTNAGG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 1407
EcoICRI GAGCTC 1 cut(s) 280
EcoNI CCTNNNNNAGG 1 cut(s) 382
EcoRI GAATTC 1 cut(s) 269
EcoRII CCWGG 2 cut(s) 383, 579
EcoT14I CCWWGG 2 cut(s) 570, 1213
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 2 cut(s) 570, 1213
FaeI CATG 5 cut(s) 236, 502, 679, 1217, 1223
FaqI GGGAC 2 cut(s) 462, 855
FatI CATG 5 cut(s) 232, 498, 675, 1213, 1219
FbaI TGATCA 1 cut(s) 237
FblI GTMKAC 1 cut(s) 858
FokI GGATG 2 cut(s) 1401, 1465
FriOI GRGCYC 1 cut(s) 282
FspBI CTAG 4 cut(s) 120, 303, 363, 729
GsaI CCCAGC 1 cut(s) 483
HaeIII GGCC 3 cut(s) 54, 938, 1020
HapII CCGG 1 cut(s) 223
HgaI GACGC 2 cut(s) 215, 1445
Hin1I GRCGYC 1 cut(s) 207
Hin1II CATG 5 cut(s) 236, 502, 679, 1217, 1223
HpaII CCGG 1 cut(s) 223
HphI GGTGA 7 cut(s) 55, 169, 548, 821, 845, 1142, 1320
Hpy166II GTNNAC 2 cut(s) 685, 859
Hpy188III TCNNGA 7 cut(s) 203, 303, 401, 556, 1005, 1191, 1465
Hpy8I GTNNAC 2 cut(s) 685, 859
HpyAV CCTTC 5 cut(s) 347, 553, 1011, 1095, 1389
HpyCH4III ACNGT 3 cut(s) 7, 689, 1275
HpyCH4IV ACGT 2 cut(s) 475, 763
HpyF3I CTNAG 7 cut(s) 134, 393, 416, 1053, 1137, 1284, 1356
HpySE526I ACGT 2 cut(s) 475, 763
Hsp92I GRCGYC 1 cut(s) 207
Hsp92II CATG 5 cut(s) 236, 502, 679, 1217, 1223
Ksp22I TGATCA 1 cut(s) 237
Kzo9I GATC 5 cut(s) 237, 343, 811, 818, 1147
LguI GCTCTTC 2 cut(s) 287, 627
LweI GCATC 6 cut(s) 706, 875, 892, 987, 1361, 1447
MabI ACCWGGT 1 cut(s) 579
MaeI CTAG 4 cut(s) 120, 303, 363, 729
MaeII ACGT 2 cut(s) 475, 763
MaeIII GTNAC 3 cut(s) 173, 1326, 1422
MalI GATC 5 cut(s) 239, 345, 813, 820, 1149
MbiI CCGCTC 1 cut(s) 57
MboI GATC 5 cut(s) 237, 343, 811, 818, 1147
MhlI GDGCHC 3 cut(s) 282, 392, 687
MlsI TGGCCA 2 cut(s) 938, 1020
MluCI AATT 6 cut(s) 269, 290, 408, 655, 1009, 1093
MluNI TGGCCA 2 cut(s) 938, 1020
MlyI GAGTC 6 cut(s) 854, 1179, 1289, 1334, 1391, 1396
MmeI TCCRAC 1 cut(s) 1257
Mox20I TGGCCA 2 cut(s) 938, 1020
MroXI GAANNNNTTC 3 cut(s) 1000, 1084, 1229
MscI TGGCCA 2 cut(s) 938, 1020
MseI TTAA 1 cut(s) 368
MslI CAYNNNNRTG 2 cut(s) 680, 1218
Msp20I TGGCCA 2 cut(s) 938, 1020
MspA1I CMGCKG 3 cut(s) 483, 872, 1385
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 3 cut(s) 224, 385, 581
MvaI CCWGG 2 cut(s) 385, 581
NciI CCSGG 1 cut(s) 224
NcoI CCATGG 1 cut(s) 1213
NdeII GATC 5 cut(s) 237, 343, 811, 818, 1147
NlaIII CATG 5 cut(s) 236, 502, 679, 1217, 1223
NlaIV GGNNCC 1 cut(s) 1081
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 173, 1326
NspI RCATGY 3 cut(s) 236, 502, 679
PaeI GCATGC 2 cut(s) 502, 679
PaeR7I CTCGAG 1 cut(s) 1407
PaqCI CACCTGC 1 cut(s) 139
PciI ACATGT 1 cut(s) 232
PciSI GCTCTTC 2 cut(s) 287, 627
PdmI GAANNNNTTC 3 cut(s) 1000, 1084, 1229
PfeI GAWTC 9 cut(s) 75, 219, 397, 493, 602, 990, 1034, 1118, 1468
PleI GAGTC 6 cut(s) 854, 1178, 1289, 1333, 1391, 1396
PpsI GAGTC 6 cut(s) 854, 1178, 1289, 1333, 1391, 1396
PscI ACATGT 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 282
Psp6I CCWGG 2 cut(s) 383, 579
PspFI CCCAGC 1 cut(s) 479
PspGI CCWGG 2 cut(s) 383, 579
PspN4I GGNNCC 1 cut(s) 1081
PspXI VCTCGAGB 1 cut(s) 1407
PstNI CAGNNNCTG 1 cut(s) 875
PvuII CAGCTG 3 cut(s) 483, 872, 1385
RsaI GTAC 4 cut(s) 474, 1247, 1363, 1377
RsaNI GTAC 4 cut(s) 473, 1246, 1362, 1376
RseI CAYNNNNRTG 2 cut(s) 680, 1218
SacI GAGCTC 1 cut(s) 282
SapI GCTCTTC 2 cut(s) 287, 627
SaqAI TTAA 1 cut(s) 368
Sau3AI GATC 5 cut(s) 237, 343, 811, 818, 1147
SchI GAGTC 6 cut(s) 854, 1179, 1289, 1334, 1391, 1396
ScrFI CCNGG 3 cut(s) 224, 385, 581
SduI GDGCHC 3 cut(s) 282, 392, 687
SexAI ACCWGGT 1 cut(s) 579
SfaNI GCATC 6 cut(s) 706, 875, 892, 987, 1361, 1447
SfcI CTRYAG 1 cut(s) 1237
Sfr274I CTCGAG 1 cut(s) 1407
SlaI CTCGAG 1 cut(s) 1407
SmiMI CAYNNNNRTG 2 cut(s) 680, 1218
SmlI CTYRAG 3 cut(s) 620, 1407, 1463
SmoI CTYRAG 3 cut(s) 620, 1407, 1463
SpeI ACTAGT 1 cut(s) 728
SphI GCATGC 2 cut(s) 502, 679
Sse9I AATT 6 cut(s) 269, 290, 408, 655, 1009, 1093
SsiI CCGC 8 cut(s) 15, 55, 113, 338, 461, 652, 864, 885
SspI AATATT 1 cut(s) 424
SspMI CTAG 4 cut(s) 120, 303, 363, 729
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 3 cut(s) 222, 383, 579
StyI CCWWGG 2 cut(s) 570, 1213
TaaI ACNGT 3 cut(s) 7, 689, 1275
TaiI ACGT 2 cut(s) 478, 766
TaqI TCGA 6 cut(s) 217, 974, 1146, 1173, 1400, 1408
TasI AATT 6 cut(s) 269, 290, 408, 655, 1009, 1093
TauI GCSGC 3 cut(s) 57, 464, 887
TfiI GAWTC 9 cut(s) 75, 219, 397, 493, 602, 990, 1034, 1118, 1468
Tru1I TTAA 1 cut(s) 368
Tru9I TTAA 1 cut(s) 368
TscAI CASTG 5 cut(s) 217, 694, 904, 1021, 1105
TseFI GTSAC 2 cut(s) 173, 1326
Tsp45I GTSAC 2 cut(s) 173, 1326
TspDTI ATGAA 8 cut(s) 262, 282, 1113, 1212, 1222, 1323, 1334, 1403
TspRI CASTG 5 cut(s) 217, 694, 904, 1021, 1105
VneI GTGCAC 1 cut(s) 683
XagI CCTNNNNNAGG 1 cut(s) 382
XapI RAATTY 2 cut(s) 269, 1009
XbaI TCTAGA 1 cut(s) 302
XceI RCATGY 3 cut(s) 236, 502, 679
XcmI CCANNNNNNNNNTGG 1 cut(s) 904
XhoI CTCGAG 1 cut(s) 1407
XmiI GTMKAC 1 cut(s) 858
XmnI GAANNNNTTC 3 cut(s) 1000, 1084, 1229
XspI CTAG 4 cut(s) 120, 303, 363, 729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.