AT5G04530

3-ketoacyl-coa synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003355
Physical Location & Seq
Reverse (-)
1291630 .. 1293723
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04530.1

Sequence Viewer

Length: 1395 bp
ATGGAGCTCTTCTCACTCTCTTCTCTTCTCCTACTCTCCACTCTCTTCGTTTTCTACATCTTCAAGTTTGTTTTCAAAAGAAGAAACCAGAGAAACTGCTACATGCTTCACTACGAGTGCTACAAGGGCATGGAAGAGAGAAAACTCGACACCGAGACTTGTGCTAAGGTCGTTCAACGAAACAAGAACTTGGGTCTTGAAGAGTACAGGTTTCTCCTTCGTACTATGGCTAGTTCCGGGATAGGAGAGGAAACGTACGGCCCAAGAAATGTGCTTGAAGGTAGAGAAGACTCTCCTACTCTCCTTGACGCTCACTCCGAAATGGACGAGATCATGTTCGACACTCTCGACAAGCTTTTCCACAAGACAAAAGGCTCAATCTCTCCTTCAGACATTGATATTCTCGTTGTCAACGTCTCACTCTTCGCTCCATCTCCTTCCCTAACCTCACGAGTCATCAACAGATACAAGATGAGAGAAGACATCAAATCCTACAATCTCTCGGGACTAGGGTGTAGCGCGAGCGTTATATCGATAGATATCGTGCAAAGGATGTTCGAAACTCGTGAAAACGCTCTCGCACTCGTGGTTAGCACCGAGACAATGGGTCCTCACTGGTACTGCGGTAAAGATAGGTCAATGATGCTCTCAAACTGTCTCTTCCGTGCCGGAGGAAGCTCTGTTCTGTTAACCAACGCAGCTAGGTTCAAGAACCAGGCTTTGATGAAGCTCGTGACAGTGGTCCGGGCTCATGTTGGCTCAGACGACGAGGCCTACTCGTGCTGCATACAAATGGAGGACAGAGATGGTCATCCAGGCTTTCTCTTAACCAAATACCTAAAAAAAGCCGCAGCTCGAGCCCTAACCAAGAACCTCCAAGTTCTCCTCCCTAGAGTCTTGCCTGTCAAGGAACTGATCCGCTATGCAATAGTTCGCGCGCTTAAACGAAGAACCTCCGCGAAAAGAGAGCCCGCGAGCTCAGGGATAGGTCTAAACCTAAAAACAGGGTTGCAACATTTCTGTATTCACCCGGGAGGAAGAGCGATTATCGAAGGAGTTGGGAAAAGCTTAGGGCTCACGGAATTCGACATCGAGCCAGCGAGAATGGCGCTTCATAGGTTCGGGAACACATCTTCTGGTGGTTTATGGTATGTTCTTGGCTACATGGAAGCTAAGAACAGACTAAAGAAAGGTGAGAAGATACTAATGATGAGTATGGGAGCTGGATTCGAGTCTAATAATTGTGTTTGGGAGGTTCTAAAGGATCTTGATGATAAGAATGTTTGGGAAGATTCAGTTGATCGATATCCGGAATTGTCGAGAATCCCTAATCCATTCGTTGAGAAGTATGATTGGATCAACGACGATACCATGAGCTTCGTTCGTGTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

464

Amino Acids

52.61

Weight (kDa)

8.75

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 27 - 316 2e-122 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 337 - 418 1.7e-09 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 340 - 399 9.2e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 5 cut(s) 521, 936, 938, 959, 974
AccIII TCCGGA 1 cut(s) 1309
AciI CCGC 5 cut(s) 624, 849, 919, 957, 972
AclWI GGATC 3 cut(s) 910, 1272, 1364
AcsI RAATTY 1 cut(s) 1082
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 4 cut(s) 206, 223, 257, 620
AgsI TTSAA 6 cut(s) 64, 76, 176, 200, 278, 709
AhdI GACNNNNNGTC 1 cut(s) 606
AjnI CCWGG 2 cut(s) 714, 814
AloI GAACNNNNNNTCC 2 cut(s) 666, 698
Alw21I GWGCWC 2 cut(s) 9, 980
Alw26I GTCTC 4 cut(s) 149, 421, 593, 662
AlwI GGATC 3 cut(s) 910, 1272, 1364
Ama87I CYCGRG 3 cut(s) 502, 855, 1030
Aor13HI TCCGGA 1 cut(s) 1309
AoxI GGCC 2 cut(s) 259, 771
ApeKI GCWGC 3 cut(s) 698, 783, 851
ApoI RAATTY 1 cut(s) 1082
AspLEI GCGC 4 cut(s) 521, 938, 940, 1111
AspS9I GGNCC 3 cut(s) 260, 608, 742
AsuC2I CCSGG 4 cut(s) 238, 746, 1031, 1032
AsuHPI GGTGA 2 cut(s) 1019, 1205
AsuII TTCGAA 1 cut(s) 558
AvaI CYCGRG 3 cut(s) 502, 855, 1030
AvaII GGWCC 2 cut(s) 608, 742
BaeI ACNNNNGTAYC 2 cut(s) 610, 643
BanII GRGCYC 6 cut(s) 9, 751, 862, 972, 980, 1077
BauI CACGAG 5 cut(s) 450, 564, 584, 731, 778
BbsI GAAGAC 2 cut(s) 294, 486
Bbv12I GWGCWC 2 cut(s) 9, 980
BbvI GCAGC 3 cut(s) 710, 770, 863
BccI CCATC 2 cut(s) 439, 800
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 143, 177
BciT130I CCWGG 2 cut(s) 716, 816
BcnI CCSGG 4 cut(s) 238, 746, 1031, 1032
BcoDI GTCTC 4 cut(s) 149, 421, 593, 662
BfaI CTAG 4 cut(s) 231, 509, 702, 891
BfoI RGCGCY 1 cut(s) 1112
BisI GCNGC 4 cut(s) 699, 784, 849, 852
BlsI GCNGC 4 cut(s) 700, 785, 850, 853
Bme1390I CCNGG 6 cut(s) 238, 716, 746, 816, 1031, 1032
Bme18I GGWCC 2 cut(s) 608, 742
BmeRI GACNNNNNGTC 1 cut(s) 606
BmeT110I CYCGRG 3 cut(s) 502, 855, 1030
BmgT120I GGNCC 3 cut(s) 260, 608, 742
BmiI GGNNCC 1 cut(s) 609
BmrFI CCNGG 6 cut(s) 238, 716, 746, 816, 1031, 1032
BmsI GCATC 1 cut(s) 633
BoxI GACNNNNGTC 1 cut(s) 740
BpiI GAAGAC 2 cut(s) 294, 486
BplI GAGNNNNNCTC 3 cut(s) 28, 761, 793
Bpu10I CCTNAGC 3 cut(s) 165, 979, 1069
Bpu14I TTCGAA 1 cut(s) 558
BpuMI CCSGG 4 cut(s) 238, 746, 1031, 1032
Bsa29I ATCGAT 2 cut(s) 533, 1303
BsaBI GATNNNNATC 3 cut(s) 539, 810, 1305
BsaJI CCNNGG 1 cut(s) 1030
BsaWI WCCGGW 1 cut(s) 1309
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bse1I ACTGG 1 cut(s) 620
Bse8I GATNNNNATC 3 cut(s) 539, 810, 1305
BseAI TCCGGA 1 cut(s) 1309
BseBI CCWGG 2 cut(s) 716, 816
BseCI ATCGAT 2 cut(s) 533, 1303
BseDI CCNNGG 1 cut(s) 1030
BseGI GGATG 2 cut(s) 558, 811
BseJI GATNNNNATC 3 cut(s) 539, 810, 1305
BseMII CTCAG 2 cut(s) 774, 993
BseNI ACTGG 1 cut(s) 620
BsePI GCGCGC 1 cut(s) 936
BseRI GAGGAG 1 cut(s) 875
BseXI GCAGC 3 cut(s) 710, 770, 863
Bsh1236I CGCG 5 cut(s) 521, 936, 938, 959, 974
BshFI GGCC 2 cut(s) 261, 773
BshVI ATCGAT 2 cut(s) 533, 1303
BsiHKAI GWGCWC 2 cut(s) 9, 980
BsiHKCI CYCGRG 3 cut(s) 502, 855, 1030
BsiSI CCGG 5 cut(s) 237, 669, 745, 1031, 1310
BsiWI CGTACG 1 cut(s) 255
BslFI GGGAC 1 cut(s) 519
BsmAI GTCTC 4 cut(s) 149, 421, 593, 662
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 2 cut(s) 261, 773
BsoBI CYCGRG 3 cut(s) 502, 855, 1030
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 6 cut(s) 9, 751, 862, 972, 980, 1077
Bsp13I TCCGGA 1 cut(s) 1309
Bsp143I GATC 5 cut(s) 330, 915, 1264, 1300, 1356
BspACI CCGC 5 cut(s) 624, 849, 919, 957, 972
BspANI GGCC 2 cut(s) 261, 773
BspCNI CTCAG 2 cut(s) 773, 992
BspDI ATCGAT 2 cut(s) 533, 1303
BspEI TCCGGA 1 cut(s) 1309
BspFNI CGCG 5 cut(s) 521, 936, 938, 959, 974
BspLI GGNNCC 1 cut(s) 609
BspPI GGATC 3 cut(s) 910, 1272, 1364
BspQI GCTCTTC 2 cut(s) 14, 1033
BspT104I TTCGAA 1 cut(s) 558
BsrI ACTGG 1 cut(s) 620
BssECI CCNNGG 1 cut(s) 1030
BssHII GCGCGC 1 cut(s) 936
BssMI GATC 5 cut(s) 330, 915, 1264, 1300, 1356
BssSI CACGAG 5 cut(s) 450, 564, 584, 731, 778
Bst2BI CACGAG 5 cut(s) 450, 564, 584, 731, 778
Bst2UI CCWGG 2 cut(s) 716, 816
Bst4CI ACNGT 2 cut(s) 656, 739
Bst6I CTCTTC 9 cut(s) 14, 25, 30, 50, 129, 195, 428, 665, 1033
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 5 cut(s) 523, 938, 972, 976, 1098
BstDEI CTNAG 5 cut(s) 165, 760, 979, 1069, 1173
BstF5I GGATG 2 cut(s) 558, 811
BstFNI CGCG 5 cut(s) 521, 936, 938, 959, 974
BstH2I RGCGCY 1 cut(s) 1112
BstHHI GCGC 4 cut(s) 521, 938, 940, 1111
BstKTI GATC 5 cut(s) 333, 918, 1267, 1303, 1359
BstMAI GTCTC 4 cut(s) 149, 421, 593, 662
BstMBI GATC 5 cut(s) 330, 915, 1264, 1300, 1356
BstMWI GCNNNNNNNGC 3 cut(s) 126, 857, 1106
BstNI CCWGG 2 cut(s) 716, 816
BstNSI RCATGY 1 cut(s) 106
BstPAI GACNNNNGTC 1 cut(s) 740
BstSCI CCNGG 6 cut(s) 236, 714, 744, 814, 1029, 1030
BstUI CGCG 5 cut(s) 521, 936, 938, 959, 974
BstV1I GCAGC 3 cut(s) 710, 770, 863
BstV2I GAAGAC 2 cut(s) 294, 486
BstX2I RGATCY 1 cut(s) 1264
BstYI RGATCY 1 cut(s) 1264
Bsu15I ATCGAT 2 cut(s) 533, 1303
BsuRI GGCC 2 cut(s) 261, 773
BsuTUI ATCGAT 2 cut(s) 533, 1303
BtsCI GGATG 2 cut(s) 558, 811
BtsIMutI CAGTG 2 cut(s) 613, 744
Cac8I GCNNGC 5 cut(s) 523, 938, 972, 976, 1098
CfoI GCGC 4 cut(s) 521, 938, 940, 1111
Cfr13I GGNCC 3 cut(s) 260, 608, 742
Cfr9I CCCGGG 1 cut(s) 1030
ClaI ATCGAT 2 cut(s) 533, 1303
CseI GACGC 1 cut(s) 317
Csp6I GTAC 4 cut(s) 205, 222, 256, 619
CviAII CATG 6 cut(s) 103, 130, 334, 752, 1165, 1372
CviQI GTAC 4 cut(s) 205, 222, 256, 619
DdeI CTNAG 5 cut(s) 165, 760, 979, 1069, 1173
DpnI GATC 5 cut(s) 332, 917, 1266, 1302, 1358
DpnII GATC 5 cut(s) 330, 915, 1264, 1300, 1356
DriI GACNNNNNGTC 1 cut(s) 606
Eam1104I CTCTTC 9 cut(s) 14, 25, 30, 50, 129, 195, 428, 665, 1033
Eam1105I GACNNNNNGTC 1 cut(s) 606
EarI CTCTTC 9 cut(s) 14, 25, 30, 50, 129, 195, 428, 665, 1033
Ecl136II GAGCTC 2 cut(s) 7, 978
Eco147I AGGCCT 1 cut(s) 773
Eco24I GRGCYC 6 cut(s) 9, 751, 862, 972, 980, 1077
Eco32I GATATC 2 cut(s) 541, 1307
Eco47I GGWCC 2 cut(s) 608, 742
Eco53kI GAGCTC 2 cut(s) 7, 978
Eco57I CTGAAG 1 cut(s) 372
Eco88I CYCGRG 3 cut(s) 502, 855, 1030
EcoICRI GAGCTC 2 cut(s) 7, 978
EcoO109I RGGNCCY 1 cut(s) 608
EcoRI GAATTC 1 cut(s) 1082
EcoRII CCWGG 2 cut(s) 714, 814
EcoRV GATATC 2 cut(s) 541, 1307
EcoT38I GRGCYC 6 cut(s) 9, 751, 862, 972, 980, 1077
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 6 cut(s) 106, 133, 337, 755, 1168, 1375
FaqI GGGAC 1 cut(s) 519
FatI CATG 6 cut(s) 102, 129, 333, 751, 1164, 1371
FauI CCCGC 1 cut(s) 979
Fnu4HI GCNGC 4 cut(s) 699, 784, 849, 852
FokI GGATG 2 cut(s) 565, 798
FriOI GRGCYC 6 cut(s) 9, 751, 862, 972, 980, 1077
Fsp4HI GCNGC 4 cut(s) 699, 784, 849, 852
FspBI CTAG 4 cut(s) 231, 509, 702, 891
GlaI GCGC 4 cut(s) 520, 937, 939, 1110
GluI GCNGC 4 cut(s) 699, 784, 849, 852
HaeII RGCGCY 1 cut(s) 1112
HaeIII GGCC 2 cut(s) 261, 773
HapII CCGG 5 cut(s) 237, 669, 745, 1031, 1310
HgaI GACGC 1 cut(s) 317
HhaI GCGC 4 cut(s) 521, 938, 940, 1111
Hin1II CATG 6 cut(s) 106, 133, 337, 755, 1168, 1375
Hin6I GCGC 4 cut(s) 519, 936, 938, 1109
HinP1I GCGC 4 cut(s) 519, 936, 938, 1109
HincII GTYRAC 2 cut(s) 412, 690
HindII GTYRAC 2 cut(s) 412, 690
HindIII AAGCTT 2 cut(s) 353, 1066
HinfI GANTC 7 cut(s) 290, 453, 894, 1227, 1232, 1292, 1323
HpaI GTTAAC 1 cut(s) 690
HpaII CCGG 5 cut(s) 237, 669, 745, 1031, 1310
HphI GGTGA 2 cut(s) 1019, 1205
Hpy166II GTNNAC 2 cut(s) 412, 690
Hpy188I TCNGA 3 cut(s) 319, 391, 763
Hpy8I GTNNAC 2 cut(s) 412, 690
Hpy99I CGWCG 2 cut(s) 770, 1367
HpyAV CCTTC 5 cut(s) 227, 272, 396, 447, 1046
HpyCH4III ACNGT 2 cut(s) 656, 739
HpyCH4IV ACGT 2 cut(s) 254, 414
HpyCH4V TGCA 4 cut(s) 547, 786, 926, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 857, 1106
HpyF3I CTNAG 5 cut(s) 165, 760, 979, 1069, 1173
HpySE526I ACGT 2 cut(s) 254, 414
Hsp92II CATG 6 cut(s) 106, 133, 337, 755, 1168, 1375
HspAI GCGC 4 cut(s) 519, 936, 938, 1109
Kpn2I TCCGGA 1 cut(s) 1309
KspAI GTTAAC 1 cut(s) 690
Kzo9I GATC 5 cut(s) 330, 915, 1264, 1300, 1356
LguI GCTCTTC 2 cut(s) 14, 1033
LmnI GCTCC 3 cut(s) 4, 433, 1220
Lsp1109I GCAGC 3 cut(s) 710, 770, 863
LweI GCATC 1 cut(s) 633
MaeI CTAG 4 cut(s) 231, 509, 702, 891
MaeII ACGT 2 cut(s) 254, 414
MaeIII GTNAC 1 cut(s) 733
MalI GATC 5 cut(s) 332, 917, 1266, 1302, 1358
MboI GATC 5 cut(s) 330, 915, 1264, 1300, 1356
MflI RGATCY 1 cut(s) 1264
MhlI GDGCHC 6 cut(s) 9, 751, 862, 972, 980, 1077
MluCI AATT 3 cut(s) 1082, 1240, 1313
MlyI GAGTC 4 cut(s) 284, 462, 903, 1241
MroI TCCGGA 1 cut(s) 1309
MseI TTAA 3 cut(s) 689, 827, 942
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 5 cut(s) 237, 669, 745, 1031, 1310
MspR9I CCNGG 6 cut(s) 238, 716, 746, 816, 1031, 1032
MvaI CCWGG 2 cut(s) 716, 816
MvnI CGCG 5 cut(s) 521, 936, 938, 959, 974
MwoI GCNNNNNNNGC 3 cut(s) 126, 857, 1106
NciI CCSGG 4 cut(s) 238, 746, 1031, 1032
NdeII GATC 5 cut(s) 330, 915, 1264, 1300, 1356
NlaIII CATG 6 cut(s) 106, 133, 337, 755, 1168, 1375
NlaIV GGNNCC 1 cut(s) 609
NmuCI GTSAC 1 cut(s) 733
NspI RCATGY 1 cut(s) 106
NspV TTCGAA 1 cut(s) 558
PaeR7I CTCGAG 1 cut(s) 855
PauI GCGCGC 1 cut(s) 936
PceI AGGCCT 1 cut(s) 773
PciSI GCTCTTC 2 cut(s) 14, 1033
PcsI WCGNNNNNNNCGW 3 cut(s) 315, 345, 411
PfeI GAWTC 3 cut(s) 1227, 1292, 1323
Pfl23II CGTACG 1 cut(s) 255
PfoI TCCNGGA 1 cut(s) 236
PkrI GCNGC 4 cut(s) 700, 785, 850, 853
PleI GAGTC 4 cut(s) 284, 461, 902, 1240
PpsI GAGTC 4 cut(s) 284, 461, 902, 1240
PpuMI RGGWCCY 1 cut(s) 608
PshAI GACNNNNGTC 1 cut(s) 740
Psp124BI GAGCTC 2 cut(s) 9, 980
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 714, 814
PspGI CCWGG 2 cut(s) 714, 814
PspLI CGTACG 1 cut(s) 255
PspN4I GGNNCC 1 cut(s) 609
PspPI GGNCC 3 cut(s) 260, 608, 742
PspPPI RGGWCCY 1 cut(s) 608
PspXI VCTCGAGB 1 cut(s) 855
PsuI RGATCY 1 cut(s) 1264
PteI GCGCGC 1 cut(s) 936
RsaI GTAC 4 cut(s) 206, 223, 257, 620
RsaNI GTAC 4 cut(s) 205, 222, 256, 619
RseI CAYNNNNRTG 1 cut(s) 791
SacI GAGCTC 2 cut(s) 9, 980
SapI GCTCTTC 2 cut(s) 14, 1033
SaqAI TTAA 3 cut(s) 689, 827, 942
SatI GCNGC 4 cut(s) 699, 784, 849, 852
Sau3AI GATC 5 cut(s) 330, 915, 1264, 1300, 1356
Sau96I GGNCC 3 cut(s) 260, 608, 742
SchI GAGTC 4 cut(s) 284, 462, 903, 1241
ScrFI CCNGG 6 cut(s) 238, 716, 746, 816, 1031, 1032
SduI GDGCHC 6 cut(s) 9, 751, 862, 972, 980, 1077
SfaNI GCATC 1 cut(s) 633
Sfr274I CTCGAG 1 cut(s) 855
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 2 cut(s) 608, 742
SlaI CTCGAG 1 cut(s) 855
SmaI CCCGGG 1 cut(s) 1032
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 1 cut(s) 855
SmoI CTYRAG 1 cut(s) 855
Sse9I AATT 3 cut(s) 1082, 1240, 1313
SseBI AGGCCT 1 cut(s) 773
SsiI CCGC 5 cut(s) 624, 849, 919, 957, 972
SspMI CTAG 4 cut(s) 231, 509, 702, 891
SstI GAGCTC 2 cut(s) 9, 980
StuI AGGCCT 1 cut(s) 773
StyD4I CCNGG 6 cut(s) 236, 714, 744, 814, 1029, 1030
TaaI ACNGT 2 cut(s) 656, 739
TaiI ACGT 2 cut(s) 257, 417
TasI AATT 3 cut(s) 1082, 1240, 1313
TatI WGTACW 1 cut(s) 204
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 3 cut(s) 1227, 1292, 1323
Tru1I TTAA 3 cut(s) 689, 827, 942
Tru9I TTAA 3 cut(s) 689, 827, 942
TscAI CASTG 2 cut(s) 620, 744
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 3 cut(s) 698, 783, 851
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 2 cut(s) 740, 1103
TspGWI ACGGA 2 cut(s) 653, 1094
TspMI CCCGGG 1 cut(s) 1030
TspRI CASTG 2 cut(s) 620, 744
VpaK11BI GGWCC 2 cut(s) 608, 742
XapI RAATTY 1 cut(s) 1082
XceI RCATGY 1 cut(s) 106
XhoI CTCGAG 1 cut(s) 855
XmaI CCCGGG 1 cut(s) 1030
XspI CTAG 4 cut(s) 231, 509, 702, 891
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.