MD07G1187000.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0003355
Physical Location & Seq
Forward (+)
26791011 .. 26792426
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1187000.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGGGATCTTGATGGCAACGTGTTTGCTGGCTCTTTTCTATGGTTTCTCCTGCCTCTGCAAGTTACTTCTCAGAAGGAGAGACCAGGCATGCTATCTGCTGGCCTACGAGTGCCACATGCCACCAGACGACATGAAGCTCAGCGCTGATTCATGCGTGAAAATCGTCTCGCGGAACAGGAATCTGGGACTGGAAGAATTCAGGTTTCTCTTGAAAACTATTACCCATTCGGGCATTGGCCAGGAAACTTACTGCGCAAGAAACATCATCCAAGGCCGAGAAGAAACTGCAACCCGAGAAGATGAACTTGCGGAGATGGATGGCATCATCTTTGACGCACTGGACAAGCTTTTCGCACGGGCTACCTCAATTTCTCGGTCGCAAATCGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCGTCCCTATCTTCACGTATAGTGAATCGTTACAAGATGAGGGACGACATCAAGAGCTTCAACCTCTCGGGAATGGGCTGCAGTGCGAGCCTCATTGCCATTGACGTTGTACAAAACTTGTTCAAGTCACACAAGAATGCGAATGCCATCGTTGTAAGCACAGAATCGTTGGCTCCTCATTGGTATTGCGGCGTAGAAAAATCCATGATGCTCACAAACTGTCTCTTCCGCTCGGGAGGGTGCGCGATGCTGCTCACGAACAAGCGAAGCCTAAAGCACCAAGCCAAACTGAAATTAAATCATTTGGTACGAATGCACACCGGCTCAAACGACGAAGCATATAACTGTTGCATACAGACAGAAGATGAGAGTGGAAATCGAGGTTTCCGCCTTACCAAATACCTAGTAAAAGCAGCAACTAAGGGTTTTACAAAGAACCTCCAAGTTCTAGCACCAAAAATACTTCCACTAAGAGAAATACTTAGGTATCTGGTGGCCTCTAGGCTTAACAATATTAGAACTAGTATTACAAGCCAAAAGCCAAAAGCAGATGGGAGACTAGGGTTGAATCTCAAGACAGGAATAGAGCACTTCTGTATTCACCCTGGTGGAAGAGCAATCATAGATGGGATTGGGAAGAGCTTAGGGCTAAGTGATTTTGACGTTGAGCCGTCAAGAATGGCTCTTCACCGGTTCGGAAACACATCGGGGGCCGGATTTTGGTACGCTTTGGGATACATGGAGGCAAAGAAGAGGCTTAAGAAGGGGAACAGGATTCTGATGAGTGGATTTGGAGCTGGTTTTAAGTGCAACAATATTGTGTGGGAAGTGATGAAGGACTTGGATGATGTCAATGTGTGGAAAGACTGCATAGATAGTTACCCTAGGGATCTAAACAAAGTCAATCCGTTCATGGAGAAGTATGGCTGGATACATGATGATTATATAGGCTTTGTTAGGTTTGACATGTCCCTATTAGTTATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

472

Amino Acids

53.02

Weight (kDa)

9.1

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 308 1.7e-115 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 336 - 418 5.2e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 339 - 410 3.5e-08 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 395
Acc16I TGCGCA 1 cut(s) 256
AccBSI CCGCTC 1 cut(s) 657
AccII CGCG 2 cut(s) 172, 671
AciI CCGC 5 cut(s) 172, 311, 615, 655, 814
AclWI GGATC 2 cut(s) 14, 1323
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 1 cut(s) 197
AfaI GTAC 3 cut(s) 537, 735, 1151
AfeI AGCGCT 1 cut(s) 145
AfiI CCNNNNNNNGG 1 cut(s) 1146
AflII CTTAAG 1 cut(s) 1184
AflIII ACRYGT 2 cut(s) 20, 1392
AgeI ACCGGT 1 cut(s) 1116
AgsI TTSAA 4 cut(s) 214, 487, 550, 994
AhlI ACTAGT 1 cut(s) 947
AjnI CCWGG 3 cut(s) 84, 240, 1030
AleI CACNNNNGTG 1 cut(s) 1032
AluBI AGCT 5 cut(s) 139, 349, 483, 1068, 1223
AluI AGCT 5 cut(s) 139, 349, 483, 1068, 1223
Alw21I GWGCWC 1 cut(s) 1017
Alw26I GTCTC 4 cut(s) 75, 172, 653, 976
AlwI GGATC 2 cut(s) 14, 1323
Ama87I CYCGRG 3 cut(s) 294, 493, 658
Aor51HI AGCGCT 1 cut(s) 145
AoxI GGCC 5 cut(s) 102, 238, 274, 921, 1137
ApeKI GCWGC 3 cut(s) 504, 676, 839
ApoI RAATTY 1 cut(s) 197
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 1116
AspA2I CCTAGG 1 cut(s) 1310
AspLEI GCGC 3 cut(s) 146, 257, 671
AspS9I GGNCC 1 cut(s) 1137
AsuHPI GGTGA 2 cut(s) 1019, 1106
AvaI CYCGRG 3 cut(s) 294, 493, 658
AvrII CCTAGG 1 cut(s) 1310
BaeI ACNNNNGTAYC 2 cut(s) 896, 929
BalI TGGCCA 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 1017
BbvI GCAGC 3 cut(s) 491, 663, 851
BccI CCATC 6 cut(s) 7, 310, 314, 581, 971, 1046
BceAI ACGGC 1 cut(s) 1081
BciT130I CCWGG 3 cut(s) 86, 242, 1032
BciVI GTATCC 2 cut(s) 1154, 1350
BcoDI GTCTC 4 cut(s) 75, 172, 653, 976
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 6 cut(s) 830, 875, 927, 948, 986, 1311
BfmI CTRYAG 1 cut(s) 505
BfoI RGCGCY 1 cut(s) 147
BfrI CTTAAG 1 cut(s) 1184
BfuI GTATCC 2 cut(s) 1154, 1350
BisI GCNGC 4 cut(s) 505, 616, 677, 840
BlnI CCTAGG 1 cut(s) 1310
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 4 cut(s) 506, 617, 678, 841
Bme1390I CCNGG 3 cut(s) 86, 242, 1032
BmeT110I CYCGRG 3 cut(s) 294, 493, 658
BmgT120I GGNCC 1 cut(s) 1137
BmiI GGNNCC 2 cut(s) 600, 1138
BmrFI CCNGG 3 cut(s) 86, 242, 1032
BmsI GCATC 3 cut(s) 333, 624, 663
Bpu10I CCTNAGC 1 cut(s) 1069
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 983
BsaAI YACGTR 1 cut(s) 443
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 3 cut(s) 271, 1030, 1310
BsaWI WCCGGW 1 cut(s) 1116
Bsc4I CCNNNNNNNGG 1 cut(s) 1146
Bse118I RCCGGY 2 cut(s) 746, 1116
Bse1I ACTGG 2 cut(s) 195, 345
Bse3DI GCAATG 1 cut(s) 519
BseBI CCWGG 3 cut(s) 86, 242, 1032
BseDI CCNNGG 3 cut(s) 271, 1030, 1310
BseGI GGATG 3 cut(s) 267, 325, 1276
BseLI CCNNNNNNNGG 1 cut(s) 1146
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 2 cut(s) 85, 154
BseNI ACTGG 2 cut(s) 195, 345
BseRI GAGGAG 1 cut(s) 591
BseXI GCAGC 3 cut(s) 491, 663, 851
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 172, 671
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 5 cut(s) 104, 240, 276, 923, 1139
BshTI ACCGGT 1 cut(s) 1116
BsiEI CGRYCG 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 1017
BsiHKCI CYCGRG 3 cut(s) 294, 493, 658
BsiSI CCGG 3 cut(s) 747, 1117, 1140
BslFI GGGAC 4 cut(s) 201, 415, 482, 1381
BslI CCNNNNNNNGG 1 cut(s) 1146
BsmAI GTCTC 4 cut(s) 75, 172, 653, 976
BsmBI CGTCTC 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 201, 415, 482, 1381
BsmI GAATGC 3 cut(s) 568, 574, 744
BsnI GGCC 5 cut(s) 104, 240, 276, 923, 1139
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 3 cut(s) 294, 493, 658
Bsp1286I GDGCHC 1 cut(s) 1017
Bsp1407I TGTACA 1 cut(s) 535
Bsp143I GATC 2 cut(s) 6, 1315
Bsp1720I GCTNAGC 1 cut(s) 140
BspACI CCGC 5 cut(s) 172, 311, 615, 655, 814
BspANI GGCC 5 cut(s) 104, 240, 276, 923, 1139
BspCNI CTCAG 2 cut(s) 84, 153
BspFNI CGCG 2 cut(s) 172, 671
BspLI GGNNCC 2 cut(s) 600, 1138
BspMAI CTGCAG 1 cut(s) 509
BspPI GGATC 2 cut(s) 14, 1323
BspQI GCTCTTC 3 cut(s) 1033, 1058, 1116
BspTI CTTAAG 1 cut(s) 1184
BspTNI GGTCTC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 2 cut(s) 746, 1116
BsrGI TGTACA 1 cut(s) 535
BsrI ACTGG 2 cut(s) 195, 345
BssAI RCCGGY 2 cut(s) 746, 1116
BssECI CCNNGG 3 cut(s) 271, 1030, 1310
BssMI GATC 2 cut(s) 6, 1315
BssT1I CCWWGG 2 cut(s) 271, 1310
Bst2UI CCWGG 3 cut(s) 86, 242, 1032
Bst4CI ACNGT 3 cut(s) 429, 647, 773
Bst6I CTCTTC 5 cut(s) 656, 1033, 1058, 1116, 1172
BstAFI CTTAAG 1 cut(s) 1184
BstAUI TGTACA 1 cut(s) 535
BstBAI YACGTR 1 cut(s) 443
BstC8I GCNNGC 4 cut(s) 30, 91, 102, 514
BstDEI CTNAG 7 cut(s) 71, 140, 846, 896, 908, 1069, 1076
BstF5I GGATG 3 cut(s) 267, 325, 1276
BstFNI CGCG 2 cut(s) 172, 671
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 3 cut(s) 146, 257, 671
BstKTI GATC 2 cut(s) 9, 1318
BstMAI GTCTC 4 cut(s) 75, 172, 653, 976
BstMBI GATC 2 cut(s) 6, 1315
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 1 cut(s) 513
BstNI CCWGG 3 cut(s) 86, 242, 1032
BstNSI RCATGY 3 cut(s) 93, 121, 1396
BstSCI CCNGG 3 cut(s) 84, 240, 1030
BstSFI CTRYAG 1 cut(s) 505
BstUI CGCG 2 cut(s) 172, 671
BstV1I GCAGC 3 cut(s) 491, 663, 851
BstX2I RGATCY 2 cut(s) 6, 1315
BstYI RGATCY 2 cut(s) 6, 1315
BsuI GTATCC 2 cut(s) 1154, 1350
BsuRI GGCC 5 cut(s) 104, 240, 276, 923, 1139
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 3 cut(s) 267, 325, 1276
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 338, 514
Cac8I GCNNGC 4 cut(s) 30, 91, 102, 514
CfoI GCGC 3 cut(s) 146, 257, 671
Cfr10I RCCGGY 2 cut(s) 746, 1116
Cfr13I GGNCC 1 cut(s) 1137
CseI GACGC 1 cut(s) 344
Csp6I GTAC 3 cut(s) 536, 734, 1150
CspAI ACCGGT 1 cut(s) 1116
CviAII CATG 9 cut(s) 90, 118, 133, 153, 631, 1165, 1339, 1361, 1393
CviQI GTAC 3 cut(s) 536, 734, 1150
DdeI CTNAG 7 cut(s) 71, 140, 846, 896, 908, 1069, 1076
DpnI GATC 2 cut(s) 8, 1317
DpnII GATC 2 cut(s) 6, 1315
DrdI GACNNNNNNGTC 1 cut(s) 395
DseDI GACNNNNNNGTC 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 238
Eam1104I CTCTTC 5 cut(s) 656, 1033, 1058, 1116, 1172
EarI CTCTTC 5 cut(s) 656, 1033, 1058, 1116, 1172
EciI GGCGGA 1 cut(s) 803
Eco130I CCWWGG 2 cut(s) 271, 1310
Eco31I GGTCTC 1 cut(s) 75
Eco47III AGCGCT 1 cut(s) 145
Eco88I CYCGRG 3 cut(s) 294, 493, 658
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 3 cut(s) 84, 240, 1030
EcoT14I CCWWGG 2 cut(s) 271, 1310
ErhI CCWWGG 2 cut(s) 271, 1310
Esp3I CGTCTC 1 cut(s) 172
FaeI CATG 9 cut(s) 93, 121, 136, 156, 634, 1168, 1342, 1364, 1396
FalI AAGNNNNNCTT 2 cut(s) 291, 323
FaqI GGGAC 4 cut(s) 201, 415, 482, 1381
FatI CATG 9 cut(s) 89, 117, 132, 152, 630, 1164, 1338, 1360, 1392
Fnu4HI GCNGC 4 cut(s) 505, 616, 677, 840
FokI GGATG 3 cut(s) 254, 332, 1283
Fsp4HI GCNGC 4 cut(s) 505, 616, 677, 840
FspBI CTAG 6 cut(s) 830, 875, 927, 948, 986, 1311
FspI TGCGCA 1 cut(s) 256
GlaI GCGC 3 cut(s) 145, 256, 670
GluI GCNGC 4 cut(s) 505, 616, 677, 840
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 5 cut(s) 104, 240, 276, 923, 1139
HapII CCGG 3 cut(s) 747, 1117, 1140
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 146, 257, 671
Hin1II CATG 9 cut(s) 93, 121, 136, 156, 634, 1168, 1342, 1364, 1396
Hin6I GCGC 3 cut(s) 144, 255, 669
HinP1I GCGC 3 cut(s) 144, 255, 669
HindIII AAGCTT 1 cut(s) 347
HinfI GANTC 6 cut(s) 149, 181, 451, 590, 994, 1201
HpaII CCGG 3 cut(s) 747, 1117, 1140
HphI GGTGA 2 cut(s) 1019, 1106
Hpy188I TCNGA 3 cut(s) 74, 1124, 1206
Hpy188III TCNNGA 8 cut(s) 10, 211, 478, 495, 660, 682, 1000, 1101
Hpy99I CGWCG 1 cut(s) 761
HpyAV CCTTC 3 cut(s) 69, 1183, 1255
HpyCH4III ACNGT 3 cut(s) 429, 647, 773
HpyCH4IV ACGT 4 cut(s) 20, 442, 531, 1089
HpyCH4V TGCA 8 cut(s) 60, 290, 425, 507, 742, 777, 1236, 1296
HpyF10VI GCNNNNNNNGC 1 cut(s) 513
HpyF3I CTNAG 7 cut(s) 71, 140, 846, 896, 908, 1069, 1076
HpySE526I ACGT 4 cut(s) 20, 442, 531, 1089
Hsp92II CATG 9 cut(s) 93, 121, 136, 156, 634, 1168, 1342, 1364, 1396
HspAI GCGC 3 cut(s) 144, 255, 669
Kzo9I GATC 2 cut(s) 6, 1315
LguI GCTCTTC 3 cut(s) 1033, 1058, 1116
LmnI GCTCC 2 cut(s) 604, 1220
Lsp1109I GCAGC 3 cut(s) 491, 663, 851
LweI GCATC 3 cut(s) 333, 624, 663
MaeI CTAG 6 cut(s) 830, 875, 927, 948, 986, 1311
MaeII ACGT 4 cut(s) 20, 442, 531, 1089
MaeIII GTNAC 4 cut(s) 63, 455, 552, 1304
MalI GATC 2 cut(s) 8, 1317
MbiI CCGCTC 1 cut(s) 657
MboI GATC 2 cut(s) 6, 1315
MflI RGATCY 2 cut(s) 6, 1315
MhlI GDGCHC 1 cut(s) 1017
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 3 cut(s) 197, 369, 719
MluNI TGGCCA 1 cut(s) 240
Mox20I TGGCCA 1 cut(s) 240
MscI TGGCCA 1 cut(s) 240
MseI TTAA 4 cut(s) 722, 933, 1185, 1230
MslI CAYNNNNRTG 2 cut(s) 561, 1032
Msp20I TGGCCA 1 cut(s) 240
MspCI CTTAAG 1 cut(s) 1184
MspI CCGG 3 cut(s) 747, 1117, 1140
MspR9I CCNGG 3 cut(s) 86, 242, 1032
Mva1269I GAATGC 3 cut(s) 568, 574, 744
MvaI CCWGG 3 cut(s) 86, 242, 1032
MvnI CGCG 2 cut(s) 172, 671
MwoI GCNNNNNNNGC 1 cut(s) 513
NdeII GATC 2 cut(s) 6, 1315
NlaIII CATG 9 cut(s) 93, 121, 136, 156, 634, 1168, 1342, 1364, 1396
NlaIV GGNNCC 2 cut(s) 600, 1138
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 552
NsbI TGCGCA 1 cut(s) 256
NspI RCATGY 3 cut(s) 93, 121, 1396
OliI CACNNNNGTG 1 cut(s) 1032
PaeI GCATGC 1 cut(s) 93
PciI ACATGT 1 cut(s) 1392
PciSI GCTCTTC 3 cut(s) 1033, 1058, 1116
PctI GAATGC 3 cut(s) 568, 574, 744
PfeI GAWTC 6 cut(s) 149, 181, 451, 590, 994, 1201
PinAI ACCGGT 1 cut(s) 1116
PkrI GCNGC 4 cut(s) 506, 617, 678, 841
Ppu21I YACGTR 1 cut(s) 443
PscI ACATGT 1 cut(s) 1392
Psp6I CCWGG 3 cut(s) 84, 240, 1030
PspGI CCWGG 3 cut(s) 84, 240, 1030
PspN4I GGNNCC 2 cut(s) 600, 1138
PspPI GGNCC 1 cut(s) 1137
PsrI GAACNNNNNNTAC 2 cut(s) 937, 969
PstI CTGCAG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 6, 1315
RsaI GTAC 3 cut(s) 537, 735, 1151
RsaNI GTAC 3 cut(s) 536, 734, 1150
RseI CAYNNNNRTG 2 cut(s) 561, 1032
SapI GCTCTTC 3 cut(s) 1033, 1058, 1116
SaqAI TTAA 4 cut(s) 722, 933, 1185, 1230
SatI GCNGC 4 cut(s) 505, 616, 677, 840
Sau3AI GATC 2 cut(s) 6, 1315
Sau96I GGNCC 1 cut(s) 1137
ScrFI CCNGG 3 cut(s) 86, 242, 1032
SduI GDGCHC 1 cut(s) 1017
SfaNI GCATC 3 cut(s) 333, 624, 663
SfcI CTRYAG 1 cut(s) 505
SmiMI CAYNNNNRTG 2 cut(s) 561, 1032
SmlI CTYRAG 2 cut(s) 998, 1184
SmoI CTYRAG 2 cut(s) 998, 1184
SpeI ACTAGT 1 cut(s) 947
SphI GCATGC 1 cut(s) 93
Sse9I AATT 3 cut(s) 197, 369, 719
SsiI CCGC 5 cut(s) 172, 311, 615, 655, 814
SspI AATATT 2 cut(s) 940, 1243
SspMI CTAG 6 cut(s) 830, 875, 927, 948, 986, 1311
StyD4I CCNGG 3 cut(s) 84, 240, 1030
StyI CCWWGG 2 cut(s) 271, 1310
TaaI ACNGT 3 cut(s) 429, 647, 773
TaiI ACGT 4 cut(s) 23, 445, 534, 1092
TaqI TCGA 3 cut(s) 387, 410, 805
TaqII GACCGA 1 cut(s) 366
TasI AATT 3 cut(s) 197, 369, 719
TatI WGTACW 1 cut(s) 535
TauI GCSGC 1 cut(s) 618
TfiI GAWTC 6 cut(s) 149, 181, 451, 590, 994, 1201
Tru1I TTAA 4 cut(s) 722, 933, 1185, 1230
Tru9I TTAA 4 cut(s) 722, 933, 1185, 1230
TscAI CASTG 2 cut(s) 345, 514
TseFI GTSAC 1 cut(s) 552
TseI GCWGC 3 cut(s) 504, 676, 839
Tsp45I GTSAC 1 cut(s) 552
TspDTI ATGAA 5 cut(s) 141, 149, 318, 1274, 1327
TspGWI ACGGA 1 cut(s) 1323
TspRI CASTG 2 cut(s) 345, 514
Vha464I CTTAAG 1 cut(s) 1184
XapI RAATTY 1 cut(s) 197
XceI RCATGY 3 cut(s) 93, 121, 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XmaJI CCTAGG 1 cut(s) 1310
XspI CTAG 6 cut(s) 830, 875, 927, 948, 986, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.