Rroxscaffold_4G00291360

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003355
Physical Location & Seq
Forward (+)
11809783 .. 11811048
1266 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_4G00291360.1

Sequence Viewer

Length: 1095 bp
ATGAAGCTCCAAACCGACACATGTGCGAAAATCGTCATGCAAAACAAGAATCTGGGGCTGGAAGATTTCAGGTTCCTCTTGAAAACCATTGTCAATTCCGGCATTGGTGAGGAAACATCCTGCCCAAGAAACATCCTCCAAAGCCGAGAAGCAACCCTAGCAGATTCACTCTCTGAGATGGATGAAACCATTTTCGATACACTTGACACGCTTTTCGCCAGTGAACCGTTACAAGATGAGGAGGATATCAAGACATTCAACCTCTCCGGAATGGGCTGCAGCGCAAGCCTAGTGGCCATTGATGTTGTCCAGGGCTTGTTCAAGTCATACAAAGACTCATACGCCCTTGTTGGAGGCTGTTCGATGCTGTTCACCAATAGAAGATACCTTAAGCACCAAGCTATCTTGAAATTGAAGACTTTGGTAAGAACACATATCGGCTCGAATGATGATGCTTACAATTCTTGCATACAGCTAGAAGATGATAGTGGTTACCTAGGTTTTCGGCTTACCAAATACCTCACACAAGCAGCAGCTCTAGCTCTCACAATAAACTTAAAAGTTCTAGTGCCAAAAGTACTTCCACTAAGGGAAATACTCCGGTACCTGGTGGAGTCTAGGCTGCTGAAGAAAAGTGGTACCAAGAGCCAAAACCTAGAAGCAGTTGGAGTTGGTTTGAATATGAAGGCCGGAATAGAGCACTTTTGTATCCACCCGGGTGGAAGAGCAATCATAGATGGGATTGGCAAGAGCTTAGCATTAAGCGAGTATGATCTCGAGCCGTCTAGAATGGCGCTTTTCCGATTTGGTAACACATCAGCTGCTGGGTTTTGGTATGCTTTAGGATACATGGAAGCCAAAAAGAGGCTCAAGAAGGGTGATAGGATTCTTATGAGTGGATTTGGAGCAGGTTTTAAGTGCAACAATATTGTTTGGGAAGTACTGAGGGACTTGGATGATGCCAATGTTTGGAAAAGCTGCATAGATAGTTACCCTCCACATATTATAGCCAACCCTTTCATGGAAAAGTTTAGTTGGCTTAATGATGAATGCCTCAACTTTGTTAGGATTGATTTCAGCAAATTGCTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

364

Amino Acids

40.66

Weight (kDa)

6.95

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 2 - 78 2.7e-13 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 81 - 117 3.3e-10 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 117 - 208 1.4e-24 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 231 - 313 2.6e-12 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 234 - 310 2.6e-09 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 899
Acc65I GGTACC 2 cut(s) 603, 638
AccB1I GGYRCC 2 cut(s) 603, 638
AccB7I CCANNNNNTGG 1 cut(s) 969
AccIII TCCGGA 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 294
AcuI CTGAAG 1 cut(s) 647
AfaI GTAC 4 cut(s) 579, 605, 640, 942
AfiI CCNNNNNNNGG 4 cut(s) 607, 864, 969, 1021
AflII CTTAAG 1 cut(s) 389
AflIII ACRYGT 1 cut(s) 20
AgsI TTSAA 6 cut(s) 82, 259, 322, 409, 415, 679
AjnI CCWGG 2 cut(s) 309, 606
AleI CACNNNNGTG 1 cut(s) 717
AluBI AGCT 8 cut(s) 7, 401, 475, 536, 542, 753, 821, 978
AluI AGCT 8 cut(s) 7, 401, 475, 536, 542, 753, 821, 978
Alw21I GWGCWC 1 cut(s) 702
AlwNI CAGNNNCTG 1 cut(s) 824
Ama87I CYCGRG 2 cut(s) 715, 776
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 2 cut(s) 294, 687
ApeKI GCWGC 7 cut(s) 276, 279, 530, 533, 622, 821, 978
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
Asp718I GGTACC 2 cut(s) 603, 638
AspA2I CCTAGG 1 cut(s) 496
AspLEI GCGC 2 cut(s) 284, 796
AsuC2I CCSGG 2 cut(s) 716, 717
AsuHPI GGTGA 3 cut(s) 119, 364, 890
AvaI CYCGRG 2 cut(s) 715, 776
AvrII CCTAGG 1 cut(s) 496
BaeI ACNNNNGTAYC 4 cut(s) 587, 620, 691, 724
BalI TGGCCA 1 cut(s) 296
BanI GGYRCC 2 cut(s) 603, 638
BbsI GAAGAC 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 7 cut(s) 263, 291, 542, 545, 609, 808, 965
BccI CCATC 2 cut(s) 172, 731
BceAI ACGGC 1 cut(s) 766
BcgI CGANNNNNNTGC 3 cut(s) 5, 39, 1069
BciT130I CCWGG 2 cut(s) 311, 608
BciVI GTATCC 2 cut(s) 719, 839
BcnI CCSGG 2 cut(s) 716, 717
BfaI CTAG 9 cut(s) 158, 290, 476, 497, 539, 566, 618, 656, 786
BfmI CTRYAG 1 cut(s) 277
BfoI RGCGCY 1 cut(s) 797
BfrI CTTAAG 1 cut(s) 389
BfuAI ACCTGC 1 cut(s) 899
BfuI GTATCC 2 cut(s) 719, 839
BisI GCNGC 7 cut(s) 277, 280, 531, 534, 623, 822, 979
BlnI CCTAGG 1 cut(s) 496
BlpI GCTNAGC 1 cut(s) 754
BlsI GCNGC 7 cut(s) 278, 281, 532, 535, 624, 823, 980
BmcAI AGTACT 2 cut(s) 579, 942
Bme1390I CCNGG 4 cut(s) 311, 608, 716, 717
BmeT110I CYCGRG 2 cut(s) 715, 776
BmiI GGNNCC 3 cut(s) 74, 605, 640
BmrFI CCNGG 4 cut(s) 311, 608, 716, 717
BmsI GCATC 3 cut(s) 354, 442, 949
BpiI GAAGAC 1 cut(s) 422
Bpu1102I GCTNAGC 1 cut(s) 754
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 2 cut(s) 716, 717
BsaJI CCNNGG 3 cut(s) 310, 496, 715
BsaWI WCCGGW 2 cut(s) 266, 600
Bsc4I CCNNNNNNNGG 4 cut(s) 607, 864, 969, 1021
Bse1I ACTGG 1 cut(s) 219
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 2 cut(s) 311, 608
BseDI CCNNGG 3 cut(s) 310, 496, 715
BseGI GGATG 4 cut(s) 116, 132, 187, 961
BseLI CCNNNNNNNGG 4 cut(s) 607, 864, 969, 1021
BseMII CTCAG 2 cut(s) 165, 935
BseNI ACTGG 1 cut(s) 219
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 7 cut(s) 263, 291, 542, 545, 609, 808, 965
BseYI CCCAGC 1 cut(s) 824
BshFI GGCC 2 cut(s) 296, 689
BshNI GGYRCC 2 cut(s) 603, 638
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 2 cut(s) 715, 776
BsiSI CCGG 5 cut(s) 99, 267, 601, 690, 716
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 4 cut(s) 607, 864, 969, 1021
BsmFI GGGAC 1 cut(s) 962
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 2 cut(s) 296, 689
BsoBI CYCGRG 2 cut(s) 715, 776
Bsp1286I GDGCHC 1 cut(s) 702
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 1 cut(s) 772
Bsp1720I GCTNAGC 1 cut(s) 754
BspANI GGCC 2 cut(s) 296, 689
BspCNI CTCAG 2 cut(s) 166, 936
BspEI TCCGGA 1 cut(s) 266
BspLI GGNNCC 3 cut(s) 74, 605, 640
BspMAI CTGCAG 1 cut(s) 281
BspMI ACCTGC 1 cut(s) 899
BspQI GCTCTTC 1 cut(s) 718
BspT107I GGYRCC 2 cut(s) 603, 638
BspTI CTTAAG 1 cut(s) 389
BsrI ACTGG 1 cut(s) 219
BssECI CCNNGG 3 cut(s) 310, 496, 715
BssMI GATC 1 cut(s) 772
BssT1I CCWWGG 1 cut(s) 496
Bst2UI CCWGG 2 cut(s) 311, 608
Bst4CI ACNGT 1 cut(s) 228
Bst6I CTCTTC 1 cut(s) 718
BstAFI CTTAAG 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 286, 1089
BstDEI CTNAG 4 cut(s) 174, 587, 754, 944
BstEII GGTNACC 1 cut(s) 491
BstF5I GGATG 4 cut(s) 116, 132, 187, 961
BstH2I RGCGCY 1 cut(s) 797
BstHHI GCGC 2 cut(s) 284, 796
BstKTI GATC 1 cut(s) 775
BstMBI GATC 1 cut(s) 772
BstMWI GCNNNNNNNGC 3 cut(s) 158, 285, 539
BstNI CCWGG 2 cut(s) 311, 608
BstNSI RCATGY 1 cut(s) 24
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 4 cut(s) 309, 606, 714, 715
BstSFI CTRYAG 1 cut(s) 277
BstV1I GCAGC 7 cut(s) 263, 291, 542, 545, 609, 808, 965
BstV2I GAAGAC 1 cut(s) 422
BstXI CCANNNNNNTGG 1 cut(s) 719
BsuI GTATCC 2 cut(s) 719, 839
BsuRI GGCC 2 cut(s) 296, 689
BtsCI GGATG 4 cut(s) 116, 132, 187, 961
BtsIMutI CAGTG 1 cut(s) 226
BveI ACCTGC 1 cut(s) 899
Cac8I GCNNGC 2 cut(s) 286, 1089
CaiI CAGNNNCTG 1 cut(s) 824
CfoI GCGC 2 cut(s) 284, 796
Cfr9I CCCGGG 1 cut(s) 715
CsiI ACCWGGT 1 cut(s) 606
Csp6I GTAC 4 cut(s) 578, 604, 639, 941
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviAII CATG 4 cut(s) 21, 37, 850, 1021
CviQI GTAC 4 cut(s) 578, 604, 639, 941
DdeI CTNAG 4 cut(s) 174, 587, 754, 944
DpnI GATC 1 cut(s) 774
DpnII GATC 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 718
EarI CTCTTC 1 cut(s) 718
Eco130I CCWWGG 1 cut(s) 496
Eco32I GATATC 1 cut(s) 247
Eco57I CTGAAG 1 cut(s) 647
Eco88I CYCGRG 2 cut(s) 715, 776
Eco91I GGTNACC 1 cut(s) 491
EcoO65I GGTNACC 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 309, 606
EcoRV GATATC 1 cut(s) 247
EcoT14I CCWWGG 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 4 cut(s) 24, 40, 853, 1024
FaqI GGGAC 1 cut(s) 962
FatI CATG 4 cut(s) 20, 36, 849, 1020
Fnu4HI GCNGC 7 cut(s) 277, 280, 531, 534, 623, 822, 979
FokI GGATG 4 cut(s) 103, 119, 194, 968
Fsp4HI GCNGC 7 cut(s) 277, 280, 531, 534, 623, 822, 979
FspBI CTAG 9 cut(s) 158, 290, 476, 497, 539, 566, 618, 656, 786
GlaI GCGC 2 cut(s) 283, 795
GluI GCNGC 7 cut(s) 277, 280, 531, 534, 623, 822, 979
GsaI CCCAGC 1 cut(s) 828
HaeII RGCGCY 1 cut(s) 797
HaeIII GGCC 2 cut(s) 296, 689
HapII CCGG 5 cut(s) 99, 267, 601, 690, 716
HhaI GCGC 2 cut(s) 284, 796
Hin1II CATG 4 cut(s) 24, 40, 853, 1024
Hin6I GCGC 2 cut(s) 282, 794
HinP1I GCGC 2 cut(s) 282, 794
HinfI GANTC 5 cut(s) 49, 164, 335, 614, 886
HpaII CCGG 5 cut(s) 99, 267, 601, 690, 716
HphI GGTGA 3 cut(s) 119, 364, 890
Hpy166II GTNNAC 2 cut(s) 224, 372
Hpy188I TCNGA 2 cut(s) 175, 803
Hpy188III TCNNGA 7 cut(s) 79, 250, 267, 406, 776, 786, 871
Hpy8I GTNNAC 2 cut(s) 224, 372
HpyAV CCTTC 2 cut(s) 679, 868
HpyCH4III ACNGT 1 cut(s) 228
HpyCH4V TGCA 5 cut(s) 40, 279, 468, 921, 981
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 285, 539
HpyF3I CTNAG 4 cut(s) 174, 587, 754, 944
Hsp92II CATG 4 cut(s) 24, 40, 853, 1024
HspAI GCGC 2 cut(s) 282, 794
Kpn2I TCCGGA 1 cut(s) 266
KpnI GGTACC 2 cut(s) 607, 642
Kzo9I GATC 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 718
LmnI GCTCC 2 cut(s) 12, 905
Lsp1109I GCAGC 7 cut(s) 263, 291, 542, 545, 609, 808, 965
LweI GCATC 3 cut(s) 354, 442, 949
MabI ACCWGGT 1 cut(s) 606
MaeI CTAG 9 cut(s) 158, 290, 476, 497, 539, 566, 618, 656, 786
MaeIII GTNAC 4 cut(s) 228, 491, 809, 989
MalI GATC 1 cut(s) 774
MboI GATC 1 cut(s) 772
MboII GAAGA 6 cut(s) 74, 393, 427, 491, 640, 735
MhlI GDGCHC 1 cut(s) 702
MlsI TGGCCA 1 cut(s) 296
MluCI AATT 4 cut(s) 94, 410, 460, 1082
MluNI TGGCCA 1 cut(s) 296
MlyI GAGTC 2 cut(s) 329, 623
MmeI TCCRAC 2 cut(s) 331, 646
Mox20I TGGCCA 1 cut(s) 296
MroI TCCGGA 1 cut(s) 266
MscI TGGCCA 1 cut(s) 296
MseI TTAA 6 cut(s) 390, 557, 761, 915, 1041, 1093
MslI CAYNNNNRTG 1 cut(s) 717
Msp20I TGGCCA 1 cut(s) 296
MspA1I CMGCKG 1 cut(s) 821
MspCI CTTAAG 1 cut(s) 389
MspI CCGG 5 cut(s) 99, 267, 601, 690, 716
MspR9I CCNGG 4 cut(s) 311, 608, 716, 717
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 2 cut(s) 311, 608
MwoI GCNNNNNNNGC 3 cut(s) 158, 285, 539
NciI CCSGG 2 cut(s) 716, 717
NdeII GATC 1 cut(s) 772
NlaIII CATG 4 cut(s) 24, 40, 853, 1024
NlaIV GGNNCC 3 cut(s) 74, 605, 640
NmeAIII GCCGAG 1 cut(s) 170
NspI RCATGY 1 cut(s) 24
OliI CACNNNNGTG 1 cut(s) 717
PaeR7I CTCGAG 1 cut(s) 776
PciI ACATGT 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 718
PctI GAATGC 1 cut(s) 1055
PfeI GAWTC 3 cut(s) 49, 164, 886
PflMI CCANNNNNTGG 1 cut(s) 969
PkrI GCNGC 7 cut(s) 278, 281, 532, 535, 624, 823, 980
PleI GAGTC 2 cut(s) 329, 622
PpsI GAGTC 2 cut(s) 329, 622
PscI ACATGT 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 309, 606
PspEI GGTNACC 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 824
PspGI CCWGG 2 cut(s) 309, 606
PspN4I GGNNCC 3 cut(s) 74, 605, 640
PstI CTGCAG 1 cut(s) 281
PstNI CAGNNNCTG 1 cut(s) 824
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 4 cut(s) 579, 605, 640, 942
RsaNI GTAC 4 cut(s) 578, 604, 639, 941
RseI CAYNNNNRTG 1 cut(s) 717
SapI GCTCTTC 1 cut(s) 718
SaqAI TTAA 6 cut(s) 390, 557, 761, 915, 1041, 1093
SatI GCNGC 7 cut(s) 277, 280, 531, 534, 623, 822, 979
Sau3AI GATC 1 cut(s) 772
ScaI AGTACT 2 cut(s) 579, 942
SchI GAGTC 2 cut(s) 329, 623
ScrFI CCNGG 4 cut(s) 311, 608, 716, 717
SduI GDGCHC 1 cut(s) 702
SexAI ACCWGGT 1 cut(s) 606
SfaNI GCATC 3 cut(s) 354, 442, 949
SfcI CTRYAG 1 cut(s) 277
Sfr274I CTCGAG 1 cut(s) 776
SlaI CTCGAG 1 cut(s) 776
SmaI CCCGGG 1 cut(s) 717
SmiMI CAYNNNNRTG 1 cut(s) 717
SmlI CTYRAG 3 cut(s) 389, 776, 869
SmoI CTYRAG 3 cut(s) 389, 776, 869
Sse9I AATT 4 cut(s) 94, 410, 460, 1082
SspI AATATT 1 cut(s) 928
SspMI CTAG 9 cut(s) 158, 290, 476, 497, 539, 566, 618, 656, 786
StyD4I CCNGG 4 cut(s) 309, 606, 714, 715
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 228
TaqI TCGA 4 cut(s) 195, 362, 443, 777
TasI AATT 4 cut(s) 94, 410, 460, 1082
TatI WGTACW 2 cut(s) 577, 940
TfiI GAWTC 3 cut(s) 49, 164, 886
Tru1I TTAA 6 cut(s) 390, 557, 761, 915, 1041, 1093
Tru9I TTAA 6 cut(s) 390, 557, 761, 915, 1041, 1093
TscAI CASTG 1 cut(s) 226
TseI GCWGC 7 cut(s) 276, 279, 530, 533, 622, 821, 978
TspDTI ATGAA 5 cut(s) 17, 198, 698, 1009, 1062
TspMI CCCGGG 1 cut(s) 715
TspRI CASTG 1 cut(s) 226
Van91I CCANNNNNTGG 1 cut(s) 969
Vha464I CTTAAG 1 cut(s) 389
XbaI TCTAGA 1 cut(s) 785
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 1 cut(s) 1018
XhoI CTCGAG 1 cut(s) 776
XmaI CCCGGG 1 cut(s) 715
XmaJI CCTAGG 1 cut(s) 496
XspI CTAG 9 cut(s) 158, 290, 476, 497, 539, 566, 618, 656, 786
ZrmI AGTACT 2 cut(s) 579, 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.