MD01G1118800.v1.1

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003355
Physical Location & Seq
Forward (+)
23305574 .. 23306908
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1118800.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGAGATCTTGATGGCAACGTGTTTGCTGGCTCTTTTCCATGGTTTTTACTGCCTCTATAACTTACTTCTCAGAAGGAGAGACCAGTCATGCTATCTGCTGGCATACGAGTGCCACATGCCTCCAGACGACATGAAGCTCAGCACTGATTCATGCGTGAAAATCGTCTTGCGGAATAGGAATCTGGGACTGGATGAGTTCAGAAATATCATCAAAGGCCGAGAAGAAAATGCAACCCTAGAAGATGAACATGCGGAGGTGGATGGCATCATCTTCGACACGCTGGACAAGCTTTTCACTAGGGCCACCTCAATTTCTCCGTCACAAATTGACATTCTTGTCGTCAATGTGTCGATGTTCTCCCCTGCACCCTCCCTATCGTCGCGTATAGTGAATTGTTACAAGATGAGGGGCAACATCAACAGCTTCAACCTCTCGGGAATGGGTTGCAGCGCGACCCTTATTGCCGTTGACGTTGTACAAAACTTGTTCAAGTCTCACAAGAACGCGAATGCCATCATTGTAAGCACGGAATCCTTAGCTCCTCATTGGTATTGTGGCATAGAAAAATCCATGATGCTCAAAAACTGTCTCTTCCGCTCGGGAGGGTGCGCGATGCTGTTCACGAACAACCAAGACCTAAAGCACCAAGCCAAGCTGAAATTAAACCATTTGGTACGAATCCACATTGGCTCGAACGACGACGCATATAACTGTACAGCTGAGGGAGTAGGGTTGAATCTCAAGACAAGAATAGAGCACTTCTGTATTCAGCCTGGGGGAAGAGCAATCATAGATGGGATTGGGGAGAGCTTAGAGCTAAGTGATTATGATGTTGAGCCGTCAAGAACGGCGCTTCACCGGTTTGGAAACACGTCGGCGGCTGGATTTTGGTACGCTTTGGGAGACATGGAGGCAAAGAAGAGGCTGAAGAAGGGGAATAGGATTCTGATGAGTGGATTTGGAGCTGGTTTCAAGTGCAACAATATTGTGTGGGAAGTGATGAAGGACTTGGATGATGTCAATGTGTGGAAAGACTGCATAGACAGTTACCCTACGGATCAAACCCTAGTCAATCCTTTCATGGAGAAGTATGGCTGGATCAATGATGATTATCTAGGCTTTGTTAAGTTTGATTTCTCCCTATTAGCTGTTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

387

Amino Acids

43.43

Weight (kDa)

6.08

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 25 - 68 4.9e-06 FAE1/Type III polyketide synthase-like protein
FAE1_CUT1_RppA PF08392 70 - 241 1.2e-71 FAE1/Type III polyketide synthase-like protein
ACP_syn_III_C PF08541 251 - 333 1e-11 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C terminal
Chal_sti_synt_C PF02797 255 - 325 2.1e-07 Chalcone and stilbene synthases, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccBSI CCGCTC 1 cut(s) 600
AccII CGCG 4 cut(s) 385, 455, 509, 614
AciI CCGC 4 cut(s) 172, 254, 598, 881
AclWI GGATC 2 cut(s) 1068, 1109
AcuI CTGAAG 1 cut(s) 950
AfaI GTAC 4 cut(s) 480, 678, 718, 896
AflIII ACRYGT 2 cut(s) 20, 873
AgeI ACCGGT 1 cut(s) 861
AgsI TTSAA 4 cut(s) 430, 493, 739, 976
AjiI CACGTC 1 cut(s) 876
AjnI CCWGG 1 cut(s) 775
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 4 cut(s) 75, 501, 596, 900
AlwI GGATC 2 cut(s) 1068, 1109
Ama87I CYCGRG 2 cut(s) 436, 601
AoxI GGCC 2 cut(s) 217, 303
ApeKI GCWGC 1 cut(s) 450
AsiGI ACCGGT 1 cut(s) 861
AspLEI GCGC 3 cut(s) 455, 614, 856
AspS9I GGNCC 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 851
AvaI CYCGRG 2 cut(s) 436, 601
Bbv12I GWGCWC 1 cut(s) 762
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 1 cut(s) 462
BccI CCATC 4 cut(s) 7, 257, 524, 791
BceAI ACGGC 3 cut(s) 452, 826, 867
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 1 cut(s) 777
BcoDI GTCTC 4 cut(s) 75, 501, 596, 900
BfaI CTAG 4 cut(s) 239, 300, 1070, 1118
BfoI RGCGCY 1 cut(s) 857
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 451, 882
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 2 cut(s) 452, 883
Bme1390I CCNGG 1 cut(s) 777
BmeT110I CYCGRG 2 cut(s) 436, 601
BmgBI CACGTC 1 cut(s) 876
BmgT120I GGNCC 1 cut(s) 303
BmrFI CCNGG 1 cut(s) 777
BmsI GCATC 3 cut(s) 276, 567, 606
BpmI CTGGAG 1 cut(s) 108
Bpu10I CCTNAGC 2 cut(s) 538, 723
Bpu1102I GCTNAGC 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 1113
BsaI GGTCTC 1 cut(s) 75
BsaJI CCNNGG 2 cut(s) 40, 776
BsaWI WCCGGW 1 cut(s) 861
Bse118I RCCGGY 1 cut(s) 861
Bse1I ACTGG 2 cut(s) 85, 195
Bse8I GATNNNNATC 1 cut(s) 1113
BseBI CCWGG 1 cut(s) 777
BseDI CCNNGG 2 cut(s) 40, 776
BseGI GGATG 3 cut(s) 199, 268, 1021
BseJI GATNNNNATC 1 cut(s) 1113
BseMII CTCAG 3 cut(s) 85, 154, 714
BseNI ACTGG 2 cut(s) 85, 195
BseRI GAGGAG 1 cut(s) 534
BseXI GCAGC 1 cut(s) 462
BsgI GTGCAG 1 cut(s) 351
Bsh1236I CGCG 4 cut(s) 385, 455, 509, 614
BshFI GGCC 2 cut(s) 219, 305
BshTI ACCGGT 1 cut(s) 861
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 2 cut(s) 436, 601
BsiSI CCGG 1 cut(s) 862
BslFI GGGAC 1 cut(s) 201
BsmAI GTCTC 4 cut(s) 75, 501, 596, 900
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 2 cut(s) 219, 305
Bso31I GGTCTC 1 cut(s) 75
BsoBI CYCGRG 2 cut(s) 436, 601
Bsp1286I GDGCHC 1 cut(s) 762
Bsp1407I TGTACA 2 cut(s) 478, 716
Bsp143I GATC 3 cut(s) 6, 1060, 1101
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 4 cut(s) 172, 254, 598, 881
BspANI GGCC 2 cut(s) 219, 305
BspCNI CTCAG 3 cut(s) 84, 153, 715
BspFNI CGCG 4 cut(s) 385, 455, 509, 614
BspPI GGATC 2 cut(s) 1068, 1109
BspQI GCTCTTC 1 cut(s) 778
BspTNI GGTCTC 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 600
BsrFI RCCGGY 1 cut(s) 861
BsrGI TGTACA 2 cut(s) 478, 716
BsrI ACTGG 2 cut(s) 85, 195
BssAI RCCGGY 1 cut(s) 861
BssECI CCNNGG 2 cut(s) 40, 776
BssMI GATC 3 cut(s) 6, 1060, 1101
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 777
Bst4CI ACNGT 3 cut(s) 590, 716, 1049
Bst6I CTCTTC 3 cut(s) 599, 778, 917
BstAUI TGTACA 2 cut(s) 478, 716
BstC8I GCNNGC 2 cut(s) 30, 102
BstDEI CTNAG 6 cut(s) 71, 140, 538, 723, 814, 821
BstDSI CCRYGG 1 cut(s) 40
BstF5I GGATG 3 cut(s) 199, 268, 1021
BstFNI CGCG 4 cut(s) 385, 455, 509, 614
BstH2I RGCGCY 1 cut(s) 857
BstHHI GCGC 3 cut(s) 455, 614, 856
BstKTI GATC 3 cut(s) 9, 1063, 1104
BstMAI GTCTC 4 cut(s) 75, 501, 596, 900
BstMBI GATC 3 cut(s) 6, 1060, 1101
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 1 cut(s) 777
BstNSI RCATGY 2 cut(s) 121, 254
BstSCI CCNGG 1 cut(s) 775
BstUI CGCG 4 cut(s) 385, 455, 509, 614
BstV1I GCAGC 1 cut(s) 462
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 2 cut(s) 219, 305
BtgI CCRYGG 1 cut(s) 40
BtgZI GCGATG 1 cut(s) 629
BtrI CACGTC 1 cut(s) 876
BtsCI GGATG 3 cut(s) 199, 268, 1021
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 30, 102
CfoI GCGC 3 cut(s) 455, 614, 856
Cfr10I RCCGGY 1 cut(s) 861
Cfr13I GGNCC 1 cut(s) 303
CseI GACGC 1 cut(s) 713
Csp6I GTAC 4 cut(s) 479, 677, 717, 895
CspAI ACCGGT 1 cut(s) 861
CviAII CATG 9 cut(s) 41, 90, 118, 133, 153, 251, 574, 910, 1084
CviQI GTAC 4 cut(s) 479, 677, 717, 895
DdeI CTNAG 6 cut(s) 71, 140, 538, 723, 814, 821
DpnI GATC 3 cut(s) 8, 1062, 1103
DpnII GATC 3 cut(s) 6, 1060, 1101
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 3 cut(s) 599, 778, 917
EarI CTCTTC 3 cut(s) 599, 778, 917
Eco130I CCWWGG 1 cut(s) 40
Eco31I GGTCTC 1 cut(s) 75
Eco57I CTGAAG 1 cut(s) 950
Eco88I CYCGRG 2 cut(s) 436, 601
EcoRII CCWGG 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 9 cut(s) 44, 93, 121, 136, 156, 254, 577, 913, 1087
FaqI GGGAC 1 cut(s) 201
FatI CATG 9 cut(s) 40, 89, 117, 132, 152, 250, 573, 909, 1083
Fnu4HI GCNGC 2 cut(s) 451, 882
FokI GGATG 3 cut(s) 206, 275, 1028
Fsp4HI GCNGC 2 cut(s) 451, 882
FspBI CTAG 4 cut(s) 239, 300, 1070, 1118
GlaI GCGC 3 cut(s) 454, 613, 855
GluI GCNGC 2 cut(s) 451, 882
GsuI CTGGAG 1 cut(s) 108
HaeII RGCGCY 1 cut(s) 857
HaeIII GGCC 2 cut(s) 219, 305
HapII CCGG 1 cut(s) 862
HgaI GACGC 1 cut(s) 713
HhaI GCGC 3 cut(s) 455, 614, 856
Hin1II CATG 9 cut(s) 44, 93, 121, 136, 156, 254, 577, 913, 1087
Hin6I GCGC 3 cut(s) 453, 612, 854
HinP1I GCGC 3 cut(s) 453, 612, 854
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 1 cut(s) 290
HinfI GANTC 6 cut(s) 149, 181, 533, 681, 739, 946
HpaII CCGG 1 cut(s) 862
HphI GGTGA 1 cut(s) 851
Hpy166II GTNNAC 2 cut(s) 472, 624
Hpy188I TCNGA 3 cut(s) 74, 203, 951
Hpy188III TCNNGA 7 cut(s) 10, 125, 438, 603, 625, 745, 846
Hpy8I GTNNAC 2 cut(s) 472, 624
Hpy99I CGWCG 4 cut(s) 385, 704, 707, 880
HpyAV CCTTC 3 cut(s) 69, 928, 1000
HpyCH4III ACNGT 3 cut(s) 590, 716, 1049
HpyCH4IV ACGT 3 cut(s) 20, 474, 875
HpyCH4V TGCA 5 cut(s) 233, 368, 450, 981, 1041
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 6 cut(s) 71, 140, 538, 723, 814, 821
HpySE526I ACGT 3 cut(s) 20, 474, 875
Hsp92II CATG 9 cut(s) 44, 93, 121, 136, 156, 254, 577, 913, 1087
HspAI GCGC 3 cut(s) 453, 612, 854
Kzo9I GATC 3 cut(s) 6, 1060, 1101
LguI GCTCTTC 1 cut(s) 778
LmnI GCTCC 2 cut(s) 547, 965
Lsp1109I GCAGC 1 cut(s) 462
LweI GCATC 3 cut(s) 276, 567, 606
MaeI CTAG 4 cut(s) 239, 300, 1070, 1118
MaeII ACGT 3 cut(s) 20, 474, 875
MaeIII GTNAC 3 cut(s) 321, 398, 1049
MalI GATC 3 cut(s) 8, 1062, 1103
MbiI CCGCTC 1 cut(s) 600
MboI GATC 3 cut(s) 6, 1060, 1101
MboII GAAGA 7 cut(s) 236, 254, 265, 586, 795, 934, 943
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 762
MluCI AATT 5 cut(s) 312, 327, 394, 662, 1156
MseI TTAA 3 cut(s) 665, 1128, 1155
MslI CAYNNNNRTG 1 cut(s) 109
MspA1I CMGCKG 1 cut(s) 722
MspI CCGG 1 cut(s) 862
MspR9I CCNGG 1 cut(s) 777
Mva1269I GAATGC 1 cut(s) 517
MvaI CCWGG 1 cut(s) 777
MvnI CGCG 4 cut(s) 385, 455, 509, 614
MwoI GCNNNNNNNGC 1 cut(s) 289
NcoI CCATGG 1 cut(s) 40
NdeII GATC 3 cut(s) 6, 1060, 1101
NlaIII CATG 9 cut(s) 44, 93, 121, 136, 156, 254, 577, 913, 1087
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 1 cut(s) 321
NspI RCATGY 2 cut(s) 121, 254
PciSI GCTCTTC 1 cut(s) 778
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 6 cut(s) 149, 181, 533, 681, 739, 946
PinAI ACCGGT 1 cut(s) 861
PkrI GCNGC 2 cut(s) 452, 883
Psp6I CCWGG 1 cut(s) 775
PspGI CCWGG 1 cut(s) 775
PspPI GGNCC 1 cut(s) 303
PsuI RGATCY 1 cut(s) 6
PvuII CAGCTG 1 cut(s) 722
RsaI GTAC 4 cut(s) 480, 678, 718, 896
RsaNI GTAC 4 cut(s) 479, 677, 717, 895
RseI CAYNNNNRTG 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 778
SaqAI TTAA 3 cut(s) 665, 1128, 1155
SatI GCNGC 2 cut(s) 451, 882
Sau3AI GATC 3 cut(s) 6, 1060, 1101
Sau96I GGNCC 1 cut(s) 303
ScrFI CCNGG 1 cut(s) 777
SduI GDGCHC 1 cut(s) 762
SfaNI GCATC 3 cut(s) 276, 567, 606
SmiMI CAYNNNNRTG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 743
SmoI CTYRAG 1 cut(s) 743
Sse9I AATT 5 cut(s) 312, 327, 394, 662, 1156
SsiI CCGC 4 cut(s) 172, 254, 598, 881
SspI AATATT 1 cut(s) 988
SspMI CTAG 4 cut(s) 239, 300, 1070, 1118
StyD4I CCNGG 1 cut(s) 775
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 3 cut(s) 590, 716, 1049
TaiI ACGT 3 cut(s) 23, 477, 878
TaqI TCGA 3 cut(s) 276, 353, 695
TasI AATT 5 cut(s) 312, 327, 394, 662, 1156
TatI WGTACW 2 cut(s) 478, 716
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 6 cut(s) 149, 181, 533, 681, 739, 946
Tru1I TTAA 3 cut(s) 665, 1128, 1155
Tru9I TTAA 3 cut(s) 665, 1128, 1155
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 1 cut(s) 450
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 5 cut(s) 141, 149, 261, 1019, 1072
TspGWI ACGGA 3 cut(s) 309, 545, 1073
TspRI CASTG 1 cut(s) 151
XceI RCATGY 2 cut(s) 121, 254
XspI CTAG 4 cut(s) 239, 300, 1070, 1118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.